<?xml version="1.0" encoding="UTF-8"?>
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<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>F6BA69</accession>
    <name>F6BA69_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 6">Isopentenyl phosphate kinase</fullName>
            <shortName evidence="6">IPK</shortName>
            <ecNumber evidence="3 6">2.7.4.26</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Metig_0202</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes the formation of isopentenyl diphosphate (IPP), the building block of all isoprenoids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 6">
            <text>ATP + isopentenyl phosphate = ADP + isopentenyl diphosphate</text>
            <dbReference type="Rhea" id="RHEA:33963"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:65078"/>
            <dbReference type="ChEBI" id="CHEBI:128769"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.26"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the isopentenyl phosphate kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.26" evidence="3 6"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95759.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798368.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0202"/>
    <dbReference type="EnsemblBacteria" id="AEF95759">
        <property type="protein sequence ID" value="AEF95759"/>
        <property type="gene ID" value="Metig_0202"/>
    </dbReference>
    <dbReference type="GeneID" id="10643037"/>
    <dbReference type="KEGG" id="mig:Metig_0202"/>
    <dbReference type="HOGENOM" id="CLU_070213_0_0_2"/>
    <dbReference type="OMA" id="HHNASEH"/>
    <dbReference type="OrthoDB" id="96284at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102043">
        <property type="term" value="F:isopentenyl phosphate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008299">
        <property type="term" value="P:isoprenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04241">
        <property type="entry name" value="AAK_FomA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1160.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036393">
        <property type="entry name" value="AceGlu_kinase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001048">
        <property type="entry name" value="Asp/Glu/Uridylate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024192">
        <property type="entry name" value="Fosfomycin_R_FomA-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43654:SF1">
        <property type="entry name" value="PTHR43654:SF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00696">
        <property type="entry name" value="AA_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016496">
        <property type="entry name" value="Kin_FomA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53633">
        <property type="entry name" value="SSF53633"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="6 10" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="AA_kinase" evidence="9">
        <location>
            <begin position="1"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="6"/>
            <end position="10"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="55"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="56"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="60"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="156"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="177"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="215"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="219"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="8">
        <location>
            <position position="15"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00017267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR016496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR016496-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR016496-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AEF95759.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="257" mass="28944" checksum="D4D36BC1EDBC6B5B" modified="2011-07-27" version="1">MLIILKLGGSILSDKNTPFSVKWDNLERFGEEIRNAMEYYKEKNEKLNLIIVHGGGSFGHPVAKKYLRDGKFCNMQKGFWEIQKAMRRFNNLVIDTLHFYDIPAVSIQPSSFVVFKKDSLIFDLSAIKELLKRDLVPVVHGDIVVDDDDNYKILSGDHILPYLTKKLNVDLSLHASDVDGVLDENGNVIEKIDKSNIDDVLKMLKDSKGIDVTGGMYLKVMEAYKMGVKTIIFNGNKRGNIYNALIGNVMGTVIIGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-09-29" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAA0</accession>
    <name>F6BAA0_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="4">AAA ATPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metig_0233</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the archaeal ATPase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95790.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798399.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF95790">
        <property type="protein sequence ID" value="AEF95790"/>
        <property type="gene ID" value="Metig_0233"/>
    </dbReference>
    <dbReference type="GeneID" id="10643068"/>
    <dbReference type="KEGG" id="mig:Metig_0233"/>
    <dbReference type="HOGENOM" id="CLU_068608_0_0_2"/>
    <dbReference type="OMA" id="EMEYILH"/>
    <dbReference type="OrthoDB" id="50712at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011579">
        <property type="entry name" value="ATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01637">
        <property type="entry name" value="ATPase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA" evidence="3">
        <location>
            <begin position="21"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="268"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEF95790.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="350" mass="41445" checksum="7FC923223130245A" modified="2011-07-27" version="1">MKFFNRDKEIKKILSILGDEPDDIYFLYGPINSGKTALINEIINNRLDKDKYVVFYINLRRYFISKYDDFIEVLFEEYEEDKKPAELIKGLIKDSPLIFGIPIPKNTLETLFSDKSEKNVFKYITNLLLSIKKEDKQPIIIMDELQKIGDLKINGFLIYELFNYFISLTKELHLCHIFCLSSDSLFIEKVYNEAMLNGRAKYLLVDDFDKETALKFMDFLSERILNKKLSNEEKELIYSYVGGKPKDIIYVIKSLKTDKLKEILNYLLNDAKQKLKYFLEDVKEENEELYKKVVDALKIFKESYEVEDITIGKKIREFLVKRNILFLDPIEGILRPQSFLIWNGIKELKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAB2</accession>
    <name>F6BAB2_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Methyltransferase type 11</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_0245</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95802.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF95802">
        <property type="protein sequence ID" value="AEF95802"/>
        <property type="gene ID" value="Metig_0245"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0245"/>
    <dbReference type="HOGENOM" id="CLU_1682704_0_0_2"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013216">
        <property type="entry name" value="Methyltransf_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08241">
        <property type="entry name" value="Methyltransf_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="125"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="domain" description="Methyltransf_11" evidence="2">
        <location>
            <begin position="37"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF95802.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="156" mass="18461" checksum="A97FE7EBB29FBF9A" modified="2011-07-27" version="1">MMFEDERKYIKKDEIYTINIGTGGEISKFIKDKGIKILEIDIDEKRSPDLVMDIQNMHLLNDNSVDVIFCLEVLEHVQNPFKAVEEIKRVLKPGGIFIGSTPFVFPIHDEPDDYFRYTKYGILNLLLYPEIISLFMFMLSPLYYIWYFRSILRMGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAB6</accession>
    <name>F6BAB6_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 6">D,D-heptose 1,7-bisphosphate phosphatase</fullName>
            <ecNumber evidence="6">3.1.3.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Metig_0250</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the HAD-like hydrolase superfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the gmhB family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.-" evidence="6"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95806.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0250"/>
    <dbReference type="EnsemblBacteria" id="AEF95806">
        <property type="protein sequence ID" value="AEF95806"/>
        <property type="gene ID" value="Metig_0250"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0250"/>
    <dbReference type="HOGENOM" id="CLU_085077_1_1_2"/>
    <dbReference type="OMA" id="EHQICLE"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006439">
        <property type="entry name" value="HAD-SF_hydro_IA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006549">
        <property type="entry name" value="HAD-SF_hydro_IIIA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004446">
        <property type="entry name" value="Heptose_bisP_phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006543">
        <property type="entry name" value="Histidinol-phos"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42891">
        <property type="entry name" value="PTHR42891"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004682">
        <property type="entry name" value="GmhB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="SSF56784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01549">
        <property type="entry name" value="HAD-SF-IA-v1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01662">
        <property type="entry name" value="HAD-SF-IIIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01656">
        <property type="entry name" value="Histidinol-ppas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="3 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00031828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR004682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEF95806.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="181" mass="20905" checksum="355F808C8477C4E9" modified="2011-07-27" version="1">MKINNGKIMNNNKAIFLDRDGVINKRLIGDYVKKIEEFELLPKVREALIEFKKMGYLLIVITNQQGIAKGIMTEEDLKVVHDYMLKLLPEIDDIFYCPHLDGTCNCRKPKNGMLLKAKEKWNIDFSRSWMIGDSESDIICGKSVGCKTIRILYDNEKTEADFIAKDLYGCVEVIKEKTYKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAD2</accession>
    <name>F6BAD2_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="6">CoB/CoM heterodisulfide reductase, subunit B</fullName>
            <ecNumber evidence="6">1.8.98.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Metig_0266</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Part of a complex that catalyzes the reversible reduction of CoM-S-S-CoB to the thiol-coenzymes H-S-CoM (coenzyme M) and H-S-CoB (coenzyme B).</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Cofactor metabolism; coenzyme M-coenzyme B heterodisulfide reduction; coenzyme B and coenzyme M from coenzyme M-coenzyme B heterodisulfide: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the HdrB family.</text>
    </comment>
    <dbReference type="EC" id="1.8.98.1" evidence="6"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95822.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0266"/>
    <dbReference type="EnsemblBacteria" id="AEF95822">
        <property type="protein sequence ID" value="AEF95822"/>
        <property type="gene ID" value="Metig_0266"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0266"/>
    <dbReference type="HOGENOM" id="CLU_052147_1_0_2"/>
    <dbReference type="OMA" id="YMPGASC"/>
    <dbReference type="UniPathway" id="UPA00647">
        <property type="reaction ID" value="UER00700"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051912">
        <property type="term" value="F:CoB--CoM heterodisulfide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015948">
        <property type="term" value="P:methanogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017678">
        <property type="entry name" value="CoB/CoM_hetero-S_Rdtase_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004017">
        <property type="entry name" value="Cys_rich_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02754">
        <property type="entry name" value="CCG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03288">
        <property type="entry name" value="CoB_CoM_SS_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0484">Methanogenesis</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CCG" evidence="5">
        <location>
            <begin position="4"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="domain" description="CCG" evidence="5">
        <location>
            <begin position="148"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEF95822.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="295" mass="32434" checksum="07D909AB16AD26E4" modified="2011-07-27" version="1">MMKYAFFLGCIMPNRYAGVEAATRTVMEKLGVELIYMPGASCCPAPGVFGSFDQKTWLTLAARNLCIAEELGVDIITVCNGCYGSLFEAAHLLHENKEALDFVNEKLDKIGKQYKGTVHVRHFAEVIYKDIGVDKIAENVVRPLNINVGVHYGCHFLKPTDVKGLGSAERPTMLDEIVEATGAKSVDYRDKMMCCGAGGGVRARELTLALDMTKEKLENMVKAGADCVVNVCPFCHLQFDRGQIEIKEKFGVEFGFPVLHLSQLLGLAFGMEPKDVALNVHFVPVDPLLKKLGLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAH5</accession>
    <name>F6BAH5_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 4">N5-carboxyaminoimidazole ribonucleotide mutase</fullName>
            <shortName evidence="3 4">N5-CAIR mutase</shortName>
            <ecNumber evidence="3 4">5.4.99.18</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">5-(carboxyamino)imidazole ribonucleotide mutase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">purE</name>
        <name evidence="7" type="ordered locus">Metig_1453</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3 4">Catalyzes the conversion of N5-carboxyaminoimidazole ribonucleotide (N5-CAIR) to 4-carboxy-5-aminoimidazole ribonucleotide (CAIR).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3 4">
            <text>5-carboxyamino-1-(5-phospho-D-ribosyl)imidazole + H(+) = 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate</text>
            <dbReference type="Rhea" id="RHEA:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58730"/>
            <dbReference type="ChEBI" id="CHEBI:77657"/>
            <dbReference type="EC" id="5.4.99.18"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 4">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate from 5-amino-1-(5-phospho-D-ribosyl)imidazole (N5-CAIR route): step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the AIR carboxylase family. Class I subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.4.99.18" evidence="3 4"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96988.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799584.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1453"/>
    <dbReference type="EnsemblBacteria" id="AEF96988">
        <property type="protein sequence ID" value="AEF96988"/>
        <property type="gene ID" value="Metig_1453"/>
    </dbReference>
    <dbReference type="GeneID" id="10644326"/>
    <dbReference type="KEGG" id="mig:Metig_1453"/>
    <dbReference type="HOGENOM" id="CLU_094982_2_0_2"/>
    <dbReference type="OMA" id="SNSIDGW"/>
    <dbReference type="OrthoDB" id="104083at2157"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00943"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034023">
        <property type="term" value="F:5-(carboxyamino)imidazole ribonucleotide mutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01929">
        <property type="entry name" value="PurE_classI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033747">
        <property type="entry name" value="PurE_ClassI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000031">
        <property type="entry name" value="PurE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024694">
        <property type="entry name" value="PurE_prokaryotes"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00731">
        <property type="entry name" value="AIRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001338">
        <property type="entry name" value="AIR_carboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01001">
        <property type="entry name" value="AIRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01162">
        <property type="entry name" value="purE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 4" id="KW-0413">Isomerase</keyword>
    <keyword evidence="1 3 4" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AIRC" evidence="6">
        <location>
            <begin position="1"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3 5">
        <location>
            <position position="8"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3 5">
        <location>
            <position position="11"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3 5">
        <location>
            <position position="38"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR001338"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR001338-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM01001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEF96988.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="157" mass="16988" checksum="9EE378023FEAE96D" modified="2011-07-27" version="1">MICIIMGSESDLKIAEKAVNVLKEFGVEFEVRVASAHRTPELVEEIVKNSNAKVFIAIAGLAAHLPGVIASLTTKPVIAVPVESKLDGMDALLSVVQMPPGIPVAAVGIDRGENAAILALEILALQDEKIAEKLVEYRKKQKEKVMKSDEKVKNMFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAI1</accession>
    <name>F6BAI1_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">SSD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Metig_1459</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96994.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799590.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1459"/>
    <dbReference type="EnsemblBacteria" id="AEF96994">
        <property type="protein sequence ID" value="AEF96994"/>
        <property type="gene ID" value="Metig_1459"/>
    </dbReference>
    <dbReference type="GeneID" id="10644332"/>
    <dbReference type="KEGG" id="mig:Metig_1459"/>
    <dbReference type="HOGENOM" id="CLU_060248_0_0_2"/>
    <dbReference type="OMA" id="EFGGTET"/>
    <dbReference type="OrthoDB" id="33339at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004869">
        <property type="entry name" value="MMPL_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000731">
        <property type="entry name" value="SSD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03176">
        <property type="entry name" value="MMPL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50156">
        <property type="entry name" value="SSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="217"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="242"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="268"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="309"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="342"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="domain" description="SSD" evidence="7">
        <location>
            <begin position="245"/>
            <end position="367"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEF96994.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="386" mass="42092" checksum="7C832D41FFEE26D8" modified="2011-07-27" version="1">MLERILKKVAQFSDEKPFLMLLIILIVTILAGISATNIKSQTAFEKMLPQDNPIIKTLYEVRDNFGGTDIITICIKLKPSDSSDKVVDIRDPRVLKAIKYLEDDLKGIDGITGVSSPVDAIITANGGVVPNDIDTVKKIYNSLPEEQRAKIFNNDFSMVVVNAYTDAGGDQKKLMRVLDDVNERIEEAPFPPGVEVVCTGTPPMRKLMDELMKESQSFTTVVGLIGVLLVLFLYFKKPLSTIMPLIPVLIAVIWTGGAMGLLGIPLDMATAGIGSLLLGMGIDYGIHLMHRYEEERKKKRPVKEAIETAVVQTGTAVMATTATTVVGFLALTLAPLPMMANLGKVCALGIFFCMIAVLTLLPAMIIIEEKYIIPLIKKLKGGNDAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAM0</accession>
    <name>F6BAM0_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Phosphoesterase RecJ domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_0278</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95834.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798443.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0278"/>
    <dbReference type="EnsemblBacteria" id="AEF95834">
        <property type="protein sequence ID" value="AEF95834"/>
        <property type="gene ID" value="Metig_0278"/>
    </dbReference>
    <dbReference type="GeneID" id="10643113"/>
    <dbReference type="KEGG" id="mig:Metig_0278"/>
    <dbReference type="HOGENOM" id="CLU_042622_0_0_2"/>
    <dbReference type="OMA" id="DMGSGQP"/>
    <dbReference type="OrthoDB" id="45290at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001667">
        <property type="entry name" value="DDH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038763">
        <property type="entry name" value="DHH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003156">
        <property type="entry name" value="DHHA1_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01368">
        <property type="entry name" value="DHH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02272">
        <property type="entry name" value="DHHA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64182">
        <property type="entry name" value="SSF64182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DHH" evidence="1">
        <location>
            <begin position="25"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="domain" description="DHHA1" evidence="2">
        <location>
            <begin position="307"/>
            <end position="423"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF95834.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="433" mass="49159" checksum="4C64C19F9FCD9BDC" modified="2011-07-27" version="1">MEKIAKVGEVAKKIKEKIEGYDGLIRVVTHYDPDGLTSGAIMVKTLLRINKKFHLTIVEHLSKEVIDELVREKQYNDALFIFCDMGSGQIEHLIENNFNAIILDHHQPKVKEVEVNGILQLNPHIFGIDGAREVSASGICYMVAREFGFYDLSVLAITGAIGDMQHKPFIGLNKFILNEGREHRYLSIIKDIIYNCYNMPIWEAITYSTNPYIRELSDKEECLKFLKNLKINPSKKELTHEEKQKLTNALIFYRKPEDFIVDRYVIKHKVNDAYYLSELLNACGRKGLTSVGIGVALEDDECIKIAKETHLKYKKELIEELKRVKPKRMKNIEYFKGKKGMIGIVASILVKDKPVLGYHEEGEKVKVSSRGNKDLVDRGLNLGKVMEVAKEFGGEGGGHDVAAGATLPKVRLEEFLKKVDEIVGEQLKQTPKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAM4</accession>
    <name>F6BAM4_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Phosphate ABC transporter, ATPase subunit</fullName>
            <ecNumber evidence="5">3.6.3.27</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Metig_0282</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.6.3.27" evidence="5"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95838.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798447.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0282"/>
    <dbReference type="EnsemblBacteria" id="AEF95838">
        <property type="protein sequence ID" value="AEF95838"/>
        <property type="gene ID" value="Metig_0282"/>
    </dbReference>
    <dbReference type="GeneID" id="10643117"/>
    <dbReference type="KEGG" id="mig:Metig_0282"/>
    <dbReference type="HOGENOM" id="CLU_000604_1_22_2"/>
    <dbReference type="OMA" id="AFMYMGD"/>
    <dbReference type="OrthoDB" id="78752at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015415">
        <property type="term" value="F:ATPase-coupled phosphate ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005315">
        <property type="term" value="F:inorganic phosphate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03260">
        <property type="entry name" value="ABC_PstB_phosphate_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005670">
        <property type="entry name" value="Phosp_transpt1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43423">
        <property type="entry name" value="PTHR43423"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00972">
        <property type="entry name" value="3a0107s01c2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="6"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="38"/>
            <end position="45"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEF95838.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="252" mass="28859" checksum="BEB7747976796709" modified="2011-07-27" version="1">MEKIKMESKNLSLWYGEKQALKNINMPIYENRITAIIGPSGCGKSTFLRCLNRMNDLIPNVRIKGEVYLDGKNIYDKDVDVVALRKRVGMVFQKPNPFPMSIYDNVAYGPRIHGIKDKDKLDEIVEWALKKAALWDEVKDDLKKSALRLSGGQQQRLCIARAIAVKPEVILMDEPCSALDPISTLKIEDLMVELKKDYTIVIVTHNMQQASRVSDYTAFFMMGELVEFDKTEKIFLEPKDKRTEEYISGKFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAM6</accession>
    <name>F6BAM6_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Radical SAM domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Metig_0284</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95840.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798449.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0284"/>
    <dbReference type="EnsemblBacteria" id="AEF95840">
        <property type="protein sequence ID" value="AEF95840"/>
        <property type="gene ID" value="Metig_0284"/>
    </dbReference>
    <dbReference type="GeneID" id="10643119"/>
    <dbReference type="KEGG" id="mig:Metig_0284"/>
    <dbReference type="HOGENOM" id="CLU_023791_0_0_2"/>
    <dbReference type="OMA" id="RCDIHYA"/>
    <dbReference type="OrthoDB" id="13164at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034471">
        <property type="entry name" value="7_8-dihydro-6-hydroxymethylpte"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034474">
        <property type="entry name" value="Methyltransferase_Class_D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43306">
        <property type="entry name" value="PTHR43306"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00385">
        <property type="entry name" value="7_8-dihydro-6-hydroxymethylpte"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="5">
        <location>
            <begin position="80"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEF95840.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="507" mass="57959" checksum="5DCD10C75CE34DBE" modified="2011-07-27" version="1">MKTISLCPICLKQISAEIKEEDGKVIIEKKCPEHGHFKDIYWGDAELYKKFNKYEYIGSIEVTNTNIEKGCPYDCGLCPNHTSTTILANIDVTNRCNLNCPICFANANHTKKIYEPTFEEIKEMMKLLRNENPPCPAIQFAGGEPTVRDDLPELIKLAREMGFIHIQIATNGVKLKNLTYLKTLKEAGLSTIYLQFDGVTEKPYLISRNRNLLPTKLKVIENCRKIGFNSVVLVPTLAKGINDDQVGSLIKFAMENIDVVRSVNFQPVSFTGRIDESKRLKHRITIPDFIKLVEEQTNGEISKEDFYPVPFVVPISLFVDTWTNMTKVKLSCHQHCGAATYVFVEDGKLIPITRFFDVEGFIELLNEAKEDLISSKLGKAKAILHIAKELPKLMDKSKAPKNLKLIELIVEILKGNYDALTNFHYNTLMIGCMHFMDPYNYDVNRVKRCVIHYATPDGRIIPFCAYNTIHRKEVEEKFSIPIEEWKERLKNRDENRGNKSISDQLVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAN3</accession>
    <name>F6BAN3_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="6">FUN14 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Metig_0291</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FUN14 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95847.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798456.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0291"/>
    <dbReference type="EnsemblBacteria" id="AEF95847">
        <property type="protein sequence ID" value="AEF95847"/>
        <property type="gene ID" value="Metig_0291"/>
    </dbReference>
    <dbReference type="GeneID" id="10643126"/>
    <dbReference type="KEGG" id="mig:Metig_0291"/>
    <dbReference type="HOGENOM" id="CLU_095425_1_2_2"/>
    <dbReference type="OMA" id="FWLQQKG"/>
    <dbReference type="OrthoDB" id="115116at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007014">
        <property type="entry name" value="FUN14"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04930">
        <property type="entry name" value="FUN14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="31"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="73"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEF95847.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="100" mass="10338" checksum="05234F532F19D7DF" modified="2011-07-27" version="1">MDIAQFIPDLGSGFVIGFVIGWAAKKAIKVLIALIGLYLLSLLYLANLGVISINENALLALIGSVENSVIAYGSQLTGLIHSISLGSGFAVGFAIGFKKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAN7</accession>
    <name>F6BAN7_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Proteasome subunit beta</fullName>
            <ecNumber evidence="9">3.4.25.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">20S proteasome beta subunit</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Proteasome core protein PsmB</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">psmB</name>
        <name evidence="11" type="ordered locus">Metig_0295</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Component of the proteasome core, a large protease complex with broad specificity involved in protein degradation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>Cleavage of peptide bonds with very broad specificity.</text>
            <dbReference type="EC" id="3.4.25.1"/>
        </reaction>
    </comment>
    <comment type="activity regulation">
        <text evidence="9">The formation of the proteasomal ATPase PAN-20S proteasome complex, via the docking of the C-termini of PAN into the intersubunit pockets in the alpha-rings, triggers opening of the gate for substrate entry. Interconversion between the open-gate and close-gate conformations leads to a dynamic regulation of the 20S proteasome proteolysis activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">The 20S proteasome core is composed of 14 alpha and 14 beta subunits that assemble into four stacked heptameric rings, resulting in a barrel-shaped structure. The two inner rings, each composed of seven catalytic beta subunits, are sandwiched by two outer rings, each composed of seven alpha subunits. The catalytic chamber with the active sites is on the inside of the barrel. Has a gated structure, the ends of the cylinder being occluded by the N-termini of the alpha-subunits. Is capped at one or both ends by the proteasome regulatory ATPase, PAN.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the peptidase T1B family.</text>
    </comment>
    <dbReference type="EC" id="3.4.25.1" evidence="9"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95851.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798460.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0295"/>
    <dbReference type="MEROPS" id="T01.002"/>
    <dbReference type="EnsemblBacteria" id="AEF95851">
        <property type="protein sequence ID" value="AEF95851"/>
        <property type="gene ID" value="Metig_0295"/>
    </dbReference>
    <dbReference type="GeneID" id="10643130"/>
    <dbReference type="KEGG" id="mig:Metig_0295"/>
    <dbReference type="HOGENOM" id="CLU_035750_7_2_2"/>
    <dbReference type="OMA" id="VDKTGPH"/>
    <dbReference type="OrthoDB" id="89767at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019774">
        <property type="term" value="C:proteasome core complex, beta-subunit complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004298">
        <property type="term" value="F:threonine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010498">
        <property type="term" value="P:proteasomal protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03764">
        <property type="entry name" value="proteasome_beta_archeal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02113_A">
        <property type="entry name" value="Proteasome_B_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019983">
        <property type="entry name" value="Pept_T1A_Psome_bsu_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000243">
        <property type="entry name" value="Pept_T1A_subB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016050">
        <property type="entry name" value="Proteasome_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023333">
        <property type="entry name" value="Proteasome_suB-type"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00141">
        <property type="entry name" value="PROTEASOME"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="SSF56235"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03634">
        <property type="entry name" value="arc_protsome_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00854">
        <property type="entry name" value="PROTEASOME_BETA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51476">
        <property type="entry name" value="PROTEASOME_BETA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0068">Autocatalytic cleavage</keyword>
    <keyword evidence="3 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 9 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 9" id="KW-0645">Protease</keyword>
    <keyword evidence="7 9 11" id="KW-0647">Proteasome</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0888">Threonine protease</keyword>
    <keyword evidence="8 9" id="KW-0865">Zymogen</keyword>
    <feature type="propeptide" id="PRO_5005052011" description="Removed in mature form; by autocatalysis" evidence="9">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5023428409" description="Proteasome subunit beta" evidence="9">
        <location>
            <begin position="11"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="9 10">
        <location>
            <position position="11"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR600243-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AEF95851.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="216" mass="23618" checksum="A18401AA10B5A88F" modified="2011-07-27" version="1">MDERAKYMKGTTTVGLICDDAVVLATDKRATMGNLIADKEAKKLYKIDDYIAMTIAGSVGDAQALVRYVSAEAKLYKMRTGKNIPPLSCATLISNILHGNRMFPFLTQLIIGGYDLLYGPRLFSLDPIGGLNEESSFTATGSGSPTAYGILELEYSKDMTLKDGLNLAIKALISAMERDAFSGNGISLAYVNKEGVTILEDEEIEKMVKKIKRKRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAN8</accession>
    <name>F6BAN8_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Proline--tRNA ligase</fullName>
            <ecNumber evidence="7">6.1.1.15</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Prolyl-tRNA synthetase</fullName>
            <shortName evidence="7">ProRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">proS</name>
        <name evidence="9" type="ordered locus">Metig_0296</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro)</text>
            <dbReference type="Rhea" id="RHEA:14305"/>
            <dbReference type="Rhea" id="RHEA-COMP:9700"/>
            <dbReference type="Rhea" id="RHEA-COMP:9702"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:60039"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78532"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.15"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="7">Consists of three domains: the N-terminal catalytic domain, the anticodon-binding domain and the C-terminal extension.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 3 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.15" evidence="7"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95852.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798461.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0296"/>
    <dbReference type="EnsemblBacteria" id="AEF95852">
        <property type="protein sequence ID" value="AEF95852"/>
        <property type="gene ID" value="Metig_0296"/>
    </dbReference>
    <dbReference type="GeneID" id="10643131"/>
    <dbReference type="KEGG" id="mig:Metig_0296"/>
    <dbReference type="HOGENOM" id="CLU_001882_4_2_2"/>
    <dbReference type="OMA" id="EVYWVTH"/>
    <dbReference type="OrthoDB" id="14454at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004827">
        <property type="term" value="F:proline-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006433">
        <property type="term" value="P:prolyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00778">
        <property type="entry name" value="ProRS_core_arch_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.110.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.800">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01571">
        <property type="entry name" value="Pro_tRNA_synth_type3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002314">
        <property type="entry name" value="aa-tRNA-synt_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004154">
        <property type="entry name" value="Anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036621">
        <property type="entry name" value="Anticodon-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002316">
        <property type="entry name" value="Pro-tRNA-ligase_IIa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004499">
        <property type="entry name" value="Pro-tRNA-ligase_IIa_arc-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017449">
        <property type="entry name" value="Pro-tRNA_synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015264">
        <property type="entry name" value="Pro-tRNA_synth_II_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033721">
        <property type="entry name" value="ProRS_core_arch_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43382">
        <property type="entry name" value="PTHR43382"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03129">
        <property type="entry name" value="HGTP_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09181">
        <property type="entry name" value="ProRS-C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00587">
        <property type="entry name" value="tRNA-synt_2b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01046">
        <property type="entry name" value="TRNASYNTHPRO"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="SSF55681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64586">
        <property type="entry name" value="SSF64586"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00408">
        <property type="entry name" value="proS_fam_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7 9" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="5 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 7 9" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AA_TRNA_LIGASE_II" evidence="8">
        <location>
            <begin position="31"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AEF95852.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="458" mass="53269" checksum="8E0EEFC0D97C0883" modified="2011-07-27" version="1">MEFSEWYSEILERAGIYDVRYPIKGCGVYLPYGFKIRRYTFEIIRKLLDETGHDEALFPMLIPEDLLAKEAEHIKGFEDEVYWVTHGGKTPLDVKLALRPTSETPIYYMMKLWVKVHTDLPIKLYQIVNTFRYETKHTRPLIRLREIMTFKEAHTAHATKEEAEEQVKKAVEIYKKFFDTLGIPYLVSKRPDWDKFPGADYTMAFDTIFPDGRTMQIGTVHNLGQNFAKTFEIIFETPDGGKDYAYQTCYGISDRVIASIIAIHGDEKGLILPPIVAPIQVVIVPLIFKGKEEMVMNKAKEIYSLLKDKFRVHLDDRDIRPGRKFNDWELKGVPLRIEIGPKDIENGKITLFRRDTGEKFQIDDNEGLIDEIEKTLNTISENMKNRAWEKFDAFIKIVEFDESKIDEIKNILSEKRGVILIPYSDDIYNEELEDKVEASVLGVTEYKGNKYIAIARTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAQ4</accession>
    <name>F6BAQ4_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_0312</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95868.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798477.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0312"/>
    <dbReference type="EnsemblBacteria" id="AEF95868">
        <property type="protein sequence ID" value="AEF95868"/>
        <property type="gene ID" value="Metig_0312"/>
    </dbReference>
    <dbReference type="GeneID" id="10643147"/>
    <dbReference type="KEGG" id="mig:Metig_0312"/>
    <dbReference type="HOGENOM" id="CLU_074324_1_0_2"/>
    <dbReference type="OMA" id="RHCEMIQ"/>
    <dbReference type="OrthoDB" id="60124at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003748">
        <property type="entry name" value="DUF169"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37954">
        <property type="entry name" value="PTHR37954"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02596">
        <property type="entry name" value="DUF169"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF95868.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="227" mass="24620" checksum="6678C33A398CE3CB" modified="2011-07-27" version="1">MSIKEKGAKLKELLGLEGSAVAVKLAKTKDEIPAGYEELGEEKRHCEMIQIARKEGKKFYAPKEKHSCKGGAYAIGVVQDAPEPLKTGKLYHKLGNFATEEGAVRTVEAIPRVKEEIYASVYAPLEEADFTPDSIVVICTPKQGLRLAQALLYKNGGRFQADFSGIQSLCADAVAAVKVRGVANATFGCNGSRKYAGIADKEVIFAFPADDLDNVIEALEHFKNVWG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAV3</accession>
    <name>F6BAV3_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Signal peptide peptidase SppA, 36K type</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metig_1506</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S49 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97040.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799634.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1506"/>
    <dbReference type="EnsemblBacteria" id="AEF97040">
        <property type="protein sequence ID" value="AEF97040"/>
        <property type="gene ID" value="Metig_1506"/>
    </dbReference>
    <dbReference type="GeneID" id="10644379"/>
    <dbReference type="KEGG" id="mig:Metig_1506"/>
    <dbReference type="HOGENOM" id="CLU_046540_0_0_2"/>
    <dbReference type="OMA" id="YVYDEIR"/>
    <dbReference type="OrthoDB" id="36138at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07023">
        <property type="entry name" value="S49_Sppa_N_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004635">
        <property type="entry name" value="Pept_S49_SppA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002142">
        <property type="entry name" value="Peptidase_S49"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01343">
        <property type="entry name" value="Peptidase_S49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="SSF52096"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00706">
        <property type="entry name" value="SppA_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_S49" evidence="3">
        <location>
            <begin position="106"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEF97040.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="308" mass="34189" checksum="F20128450CE3451F" modified="2011-07-27" version="1">MRKLYIAMGFIFILILALVVGGTLILFSDFDFGRGNIAVINIEGPIVLKSEDSGLFGHRKMGALDYIDLLDRAEKDSNIKAILLKINSPGGEVIASEKLARKVKEVAKKKPVVAYIETIGASGAYMVACPADYIIAEKHSIVGSIGVRMDVLHYYGLMKKLGINVTVIKAGKYKDIASPYRPMTEEEREYLEKMINETYMDFVIWVAENRNLTINETLKIANGKIYMGSDAKKVGLVDEVGTEEDAINITAKLANITTPKVVEYKGEDFGLFNVLYSLLYSFGYGIGEGLGEVLVKMDYKPYKTYIMN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-09-29" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAW5</accession>
    <name>F6BAW5_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1518</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97052.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF97052">
        <property type="protein sequence ID" value="AEF97052"/>
        <property type="gene ID" value="Metig_1518"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_1518"/>
    <dbReference type="HOGENOM" id="CLU_570630_0_0_2"/>
    <dbReference type="OMA" id="SCSAWFE"/>
    <dbReference type="OrthoDB" id="29789at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="458"/>
            <end position="476"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF97052.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="477" mass="53499" checksum="D01D27A4A85A42AD" modified="2011-07-27" version="1">MVSIKKILMSCLILLLISSVYAVSDVKITPSNPKVGDIITITGKANPNEDINCQAWFEISPSIVIQPYFLHKMYNVEIPSTPNAFKVIGENVDRLYINIKMGIWVTKSATANSDGIAVISKSNVPAGTYDMEIGGTIKDPSKPVKLKIIASTTIKADENGNFKYSYKANNIPEGTVVHLNIGGVSRDVLIGGDTPTPPTTVTDNTTTTDKVLDKEPPKIIVLSPSKREFNTDKVDFDIIVEDKSSYSVKIYLNGKEIKYKKEDNHLFGTLYLNPGENELKIEAEDKFNNKNEVILKLIYTYNKISENKEEQSNKNTNEIKEIISEKKTEKNNNQENIVYGTVIKKVGNATLIIEDGTKISKEGEINIEEVPIPNTTISYLITPEDAEFSKPLELRIKIKNKNISVLYYDKSLNSWINVPYTIDEGEVIIKINKGGYYAIKELNLNDDKSSIFDNIRNTFVVFFNIILLIIKMIFGFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>F6BB19</accession>
    <name>F6BB19_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_1572</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97106.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799700.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1572"/>
    <dbReference type="EnsemblBacteria" id="AEF97106">
        <property type="protein sequence ID" value="AEF97106"/>
        <property type="gene ID" value="Metig_1572"/>
    </dbReference>
    <dbReference type="GeneID" id="10644445"/>
    <dbReference type="KEGG" id="mig:Metig_1572"/>
    <dbReference type="HOGENOM" id="CLU_1976537_0_0_2"/>
    <dbReference type="OMA" id="EYHYYKL"/>
    <dbReference type="OrthoDB" id="96519at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF97106.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="128" mass="15372" checksum="4BB8E7DC841E1005" modified="2011-07-27" version="1">MKYIRAKSKEDLIKKIKETEMDDNEVYINQELNRDVVIELLENWEIDKIYLPKSKYQRTSKKIINALKEIGVEVKSIDAKCGRPTNKDELIKKYMDKHPKEIAEITSIPLKTVEYHYYKLRKKNKKRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>F6BB20</accession>
    <name>F6BB20_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Nucleotide binding protein PINc</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Metig_1573</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97107.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799701.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1573"/>
    <dbReference type="EnsemblBacteria" id="AEF97107">
        <property type="protein sequence ID" value="AEF97107"/>
        <property type="gene ID" value="Metig_1573"/>
    </dbReference>
    <dbReference type="GeneID" id="10644446"/>
    <dbReference type="KEGG" id="mig:Metig_1573"/>
    <dbReference type="HOGENOM" id="CLU_109674_0_0_2"/>
    <dbReference type="OMA" id="GCGREFD"/>
    <dbReference type="OrthoDB" id="102178at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004518">
        <property type="term" value="F:nuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09876">
        <property type="entry name" value="PIN_Nob1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039907">
        <property type="entry name" value="NOB1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002716">
        <property type="entry name" value="PIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033411">
        <property type="entry name" value="Ribonuclease_PIN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12814">
        <property type="entry name" value="PTHR12814"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17146">
        <property type="entry name" value="PIN_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00670">
        <property type="entry name" value="PINc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="SSF88723"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0540">Nuclease</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PINc" evidence="4">
        <location>
            <begin position="2"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEF97107.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="175" mass="19968" checksum="C97CA10E049829F1" modified="2011-07-27" version="1">MKPKILDASAFIHDYNPILEEGEHYTTYDIISEVESRAEIVKQSLNLGKLKAMEPSVKNILKVKDMVSKTGDALSEADIGILALALDLDGVLYTDDYGIQNVARKLGVEVRSIITEGVKEEFIWRKVCKGCKKMYPMEHEEDVCEICGGILERKMVKGRLRKKHKAKRKKKKKMW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>F6BB43</accession>
    <name>F6BB43_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Helicase domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Metig_0372</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95928.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798537.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0372"/>
    <dbReference type="EnsemblBacteria" id="AEF95928">
        <property type="protein sequence ID" value="AEF95928"/>
        <property type="gene ID" value="Metig_0372"/>
    </dbReference>
    <dbReference type="GeneID" id="10643209"/>
    <dbReference type="KEGG" id="mig:Metig_0372"/>
    <dbReference type="HOGENOM" id="CLU_002513_3_1_2"/>
    <dbReference type="OMA" id="HRAVGNY"/>
    <dbReference type="OrthoDB" id="3323at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043138">
        <property type="term" value="F:3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004518">
        <property type="term" value="F:nuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12089">
        <property type="entry name" value="Hef_ID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006166">
        <property type="entry name" value="ERCC4_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039686">
        <property type="entry name" value="FANCM/Mph1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041755">
        <property type="entry name" value="Hef_ID"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003583">
        <property type="entry name" value="Hlx-hairpin-Hlx_DNA-bd_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011335">
        <property type="entry name" value="Restrct_endonuc-II-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010994">
        <property type="entry name" value="RuvA_2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14025:SF20">
        <property type="entry name" value="PTHR14025:SF20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02732">
        <property type="entry name" value="ERCC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00891">
        <property type="entry name" value="ERCC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00278">
        <property type="entry name" value="HhH1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47781">
        <property type="entry name" value="SSF47781"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52980">
        <property type="entry name" value="SSF52980"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0347">Helicase</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="4">
        <location>
            <begin position="22"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="5">
        <location>
            <begin position="334"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="484"/>
            <end position="519"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEF95928.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="791" mass="90707" checksum="4F34FFDC27B38E71" modified="2011-07-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-06-02" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>F6BB56</accession>
    <name>F6BB56_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0385</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95941.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798550.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF95941">
        <property type="protein sequence ID" value="AEF95941"/>
        <property type="gene ID" value="Metig_0385"/>
    </dbReference>
    <dbReference type="GeneID" id="10643222"/>
    <dbReference type="KEGG" id="mig:Metig_0385"/>
    <dbReference type="HOGENOM" id="CLU_151706_0_0_2"/>
    <dbReference type="OMA" id="DYENCGL"/>
    <dbReference type="OrthoDB" id="125673at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="14"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="55"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="96"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF95941.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="124" mass="12438" checksum="C17713695C77D492" modified="2011-07-27" version="1">MVTFDKEKMLNPSIIGGIVNGVLGTFCCLGYIVGGAVASHLYVNAGGSLDYENCGLVGAVSGVIGGVIAAILNFIIATSFMSMFGGVKAFVMTGSLAIGLISGAIFGAILGAVGGILYVVIKNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-06-02" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>F6BB58</accession>
    <name>F6BB58_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0387</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95943.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF95943">
        <property type="protein sequence ID" value="AEF95943"/>
        <property type="gene ID" value="Metig_0387"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0387"/>
    <dbReference type="HOGENOM" id="CLU_2010211_0_0_2"/>
    <dbReference type="OMA" id="VWLYALR"/>
    <dbReference type="OrthoDB" id="129715at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="45"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="71"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="101"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF95943.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="136" mass="14532" checksum="160421BF9AC7BF60" modified="2011-07-27" version="1">MGMGGIFVLSILSILILGIIFIALIIAGIFLYFGCKLANIEDVSLGKAIVAVVGGGILSFIVSSIFSAVPVIGGILSFVFGILSYVWVIKVIFNTNWSKAFLAWLMAIVVEVVVMFIIAFIIAFIFGVGILSLMHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>F6BB59</accession>
    <name>F6BB59_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Zinc finger SWIM domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_0388</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95944.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798553.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0388"/>
    <dbReference type="EnsemblBacteria" id="AEF95944">
        <property type="protein sequence ID" value="AEF95944"/>
        <property type="gene ID" value="Metig_0388"/>
    </dbReference>
    <dbReference type="GeneID" id="10643225"/>
    <dbReference type="KEGG" id="mig:Metig_0388"/>
    <dbReference type="HOGENOM" id="CLU_1207638_0_0_2"/>
    <dbReference type="OMA" id="CKHAYAL"/>
    <dbReference type="OrthoDB" id="60950at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007527">
        <property type="entry name" value="Znf_SWIM"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04434">
        <property type="entry name" value="SWIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50966">
        <property type="entry name" value="ZF_SWIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SWIM-type" evidence="2">
        <location>
            <begin position="40"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF95944.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="234" mass="27803" checksum="4481378D9A737FD5" modified="2011-07-27" version="1">MHYDNKIVERGREYYKNGLVKFCVKFEDFLYGEVVGGDVYDVKVDLSNYFGSCSCPYKYNCKHAYALIEAYKNNNYIDGNELFNSLSKKPKEEILEILKSIILKHNLWDDLIGKEDNLVEKGKAILKLIPVERKNIYTFKSFLRNQFIKNARDDELLEMLEEIANFDYFDYEDRDVVEIIESIVVEIFERGNKKTIKKALELSNKYKYALWIIREYFYDYCIEAGLVDDFAEKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>F6BB74</accession>
    <name>F6BB74_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metig_0403</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95959.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798568.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0403"/>
    <dbReference type="EnsemblBacteria" id="AEF95959">
        <property type="protein sequence ID" value="AEF95959"/>
        <property type="gene ID" value="Metig_0403"/>
    </dbReference>
    <dbReference type="GeneID" id="10643240"/>
    <dbReference type="KEGG" id="mig:Metig_0403"/>
    <dbReference type="HOGENOM" id="CLU_037276_1_0_2"/>
    <dbReference type="OMA" id="QIDCACA"/>
    <dbReference type="OrthoDB" id="51913at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031167">
        <property type="entry name" value="G_OBG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013646">
        <property type="entry name" value="MMR_HSR1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004095">
        <property type="entry name" value="TGS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012676">
        <property type="entry name" value="TGS-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08438">
        <property type="entry name" value="MMR_HSR1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02824">
        <property type="entry name" value="TGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81271">
        <property type="entry name" value="SSF81271"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51710">
        <property type="entry name" value="G_OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51880">
        <property type="entry name" value="TGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="OBG-type G" evidence="2">
        <location>
            <begin position="2"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="domain" description="TGS" evidence="3">
        <location>
            <begin position="314"/>
            <end position="390"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEF95959.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="392" mass="43949" checksum="F5ABC00D257744F8" modified="2011-07-27" version="1">MAILGLVGKPNVGKSTMFNAMTEKVVDIANYPFTTIDPNIGTSYVITDCPCKELGVKCNPQNSKCIDGKRYIPVEIIDVAGLVPGAHEGKGMGNKFLDDLRQADAFILVVDASGKTDLEGNPTENHDPVEDVKFLLNEIDMWIYGILTKNWEKLARRAQQEKNIVKVLANQLSGLNITEDDVKVAIRDLDESPIKWTDEDLLSLAKKLRKISKPMIIAANKADHPDAKENIERLKKEFKDYIVIPTSAEIELALRKAEKAGLIKYDGKDFEIVDENKLNEQQKNAFTYIKEFLKTYGGTGVQECINRAYFDLLDMIVVYPVEDENKFCDKKGNILPDAFLVKKGTTAKELAYKIHTEIGEKFIYAVDARKKMRIGADYELKHGDIIKIVSAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>F6BB78</accession>
    <name>F6BB78_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Coenzyme A biosynthesis bifunctional protein CoaBC</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">DNA/pantothenate metabolism flavoprotein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="3">Phosphopantothenoylcysteine synthetase/decarboxylase</fullName>
            <shortName evidence="3">PPCS-PPCDC</shortName>
        </alternativeName>
        <domain>
            <recommendedName>
                <fullName evidence="3">Phosphopantothenoylcysteine decarboxylase</fullName>
                <shortName evidence="3">PPC decarboxylase</shortName>
                <shortName evidence="3">PPC-DC</shortName>
                <ecNumber evidence="3">4.1.1.36</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="3">CoaC</fullName>
            </alternativeName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="3">Phosphopantothenate--cysteine ligase</fullName>
                <ecNumber evidence="3">6.3.2.5</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="3">CoaB</fullName>
            </alternativeName>
            <alternativeName>
                <fullName evidence="3">Phosphopantothenoylcysteine synthetase</fullName>
                <shortName evidence="3">PPC synthetase</shortName>
                <shortName evidence="3">PPC-S</shortName>
            </alternativeName>
        </domain>
    </protein>
    <gene>
        <name evidence="3" type="primary">coaBC</name>
        <name evidence="6" type="ordered locus">Metig_0407</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes two sequential steps in the biosynthesis of coenzyme A. In the first step cysteine is conjugated to 4'-phosphopantothenate to form 4-phosphopantothenoylcysteine. In the second step the latter compound is decarboxylated to form 4'-phosphopantotheine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>(R)-4'-phosphopantothenate + CTP + L-cysteine = CMP + diphosphate + H(+) + N-[(R)-4-phosphopantothenoyl]-L-cysteine</text>
            <dbReference type="Rhea" id="RHEA:19397"/>
            <dbReference type="ChEBI" id="CHEBI:10986"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:59458"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="EC" id="6.3.2.5"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>H(+) + N-[(R)-4-phosphopantothenoyl]-L-cysteine = (R)-4'-phosphopantetheine + CO2</text>
            <dbReference type="Rhea" id="RHEA:16793"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:59458"/>
            <dbReference type="ChEBI" id="CHEBI:61723"/>
            <dbReference type="EC" id="4.1.1.36"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
        <text evidence="3">Binds 1 FMN per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Cofactor biosynthesis; coenzyme A biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the C-terminal section; belongs to the PPC synthetase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the N-terminal section; belongs to the HFCD (homo-oligomeric flavin containing Cys decarboxylase) superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.36" evidence="3"/>
    <dbReference type="EC" id="6.3.2.5" evidence="3"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95963.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0407"/>
    <dbReference type="EnsemblBacteria" id="AEF95963">
        <property type="protein sequence ID" value="AEF95963"/>
        <property type="gene ID" value="Metig_0407"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0407"/>
    <dbReference type="HOGENOM" id="CLU_033319_0_3_2"/>
    <dbReference type="OMA" id="LDMIVAN"/>
    <dbReference type="UniPathway" id="UPA00241"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004632">
        <property type="term" value="F:phosphopantothenate--cysteine ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004633">
        <property type="term" value="F:phosphopantothenoylcysteine decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015937">
        <property type="term" value="P:coenzyme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015941">
        <property type="term" value="P:pantothenate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1950">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02225">
        <property type="entry name" value="CoaBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035929">
        <property type="entry name" value="CoaB-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005252">
        <property type="entry name" value="CoaBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007085">
        <property type="entry name" value="DNA/pantothenate-metab_flavo_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036551">
        <property type="entry name" value="Flavin_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003382">
        <property type="entry name" value="Flavoprotein"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04127">
        <property type="entry name" value="DFP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02441">
        <property type="entry name" value="Flavoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102645">
        <property type="entry name" value="SSF102645"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52507">
        <property type="entry name" value="SSF52507"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00521">
        <property type="entry name" value="coaBC_dfp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3" id="KW-0288">FMN</keyword>
    <keyword evidence="3 6" id="KW-0436">Ligase</keyword>
    <keyword evidence="2 3" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Flavoprotein" evidence="4">
        <location>
            <begin position="27"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="domain" description="DFP" evidence="5">
        <location>
            <begin position="196"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="region of interest" description="Phosphopantothenoylcysteine decarboxylase" evidence="3">
        <location>
            <begin position="1"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="region of interest" description="Phosphopantothenate--cysteine ligase" evidence="3">
        <location>
            <begin position="199"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="286"/>
        </location>
        <ligand>
            <name>CTP</name>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="296"/>
        </location>
        <ligand>
            <name>CTP</name>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="330"/>
        </location>
        <ligand>
            <name>CTP</name>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEF95963.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="411" mass="46127" checksum="F3D78606542E678A" modified="2011-07-27" version="1">MPKNFKVINIHPTKKLKGTKSKLLENKKIVVGVTSSIAAIETPKLMRELIRHGAEVYCIITEETKKIIGKDALTFGCGNEVMEEITGNIEHVALYDECDAMIIYPATANIISKIALNIADNIVNTTAMMFLEKKPLFIVPAMHENMLNAIKEHIEKLKSKENVYFISPKMEEEKAKVASIEDVVGFVIEKIGNKMDKKVLILNGGTAEFIDKVRVISNLSSGKTGVALAEAFCREGFDVEVINALGLTPPYYIKTHKVLTAEEMLNKALEIGKDADIIISCAAISDYKPESTSDKKISSENEELTIKLKRNPKVLEELRKEFKDKIIIGFKAEYGIEEEELIKKAKDRMKKYGLDMIIANDLSKHYFGDDYNEVIAISKDNTIKKIKGKKSEIAKEIVSMVKKLIIPRRCQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-06-02" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>F6BB85</accession>
    <name>F6BB85_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0414</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95970.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798579.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF95970">
        <property type="protein sequence ID" value="AEF95970"/>
        <property type="gene ID" value="Metig_0414"/>
    </dbReference>
    <dbReference type="GeneID" id="10643251"/>
    <dbReference type="KEGG" id="mig:Metig_0414"/>
    <dbReference type="HOGENOM" id="CLU_122697_0_0_2"/>
    <dbReference type="OMA" id="IEKNEMP"/>
    <dbReference type="OrthoDB" id="86386at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF95970.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="181" mass="20747" checksum="191CA3D89423869B" modified="2011-07-27" version="1">MEGYILFIISLISGFLGVILIWKTFENLNKSKIREERAKEEEEIIEGLKELKSYIAPEQKKASKEYDLLEIAFEHDILDITIANEEGLPIASTLADSEELAAKYSGIYQYIKNFMKKDIVKVSIKDKDGYVYIISISKGNIPLYLIINTKIELSQFSEKSLLRKILPLLDKYLGQNFDGNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-06-02" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>F6BB92</accession>
    <name>F6BB92_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_0421</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95977.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798586.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF95977">
        <property type="protein sequence ID" value="AEF95977"/>
        <property type="gene ID" value="Metig_0421"/>
    </dbReference>
    <dbReference type="GeneID" id="10643258"/>
    <dbReference type="KEGG" id="mig:Metig_0421"/>
    <dbReference type="HOGENOM" id="CLU_1363649_0_0_2"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF95977.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="200" mass="22492" checksum="D4E79CD8F07023C9" modified="2011-07-27" version="1">MDNYEKLIKKIAIENIIVFSGIAKEYGLNITTYDTEGNMIYPSYVCNPICSIIKGTDCNKICQKANQVSYELVKKFLNSDERLIAEDFASRNIIIVKEDKLPPIIVTSCGSKFGKYMLPIMIDNEVVGCCGVCGVYLKGEKIDLDYISECSGVEARKFEIILNEVLSPISEKKITNLLAEMIKIINSTKKQKKFKLPPLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBB1</accession>
    <name>F6BBB1_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Water Stress and Hypersensitive response domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_1585</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LEA type 2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97118.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799711.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF97118">
        <property type="protein sequence ID" value="AEF97118"/>
        <property type="gene ID" value="Metig_1585"/>
    </dbReference>
    <dbReference type="GeneID" id="25395060"/>
    <dbReference type="KEGG" id="mig:Metig_1585"/>
    <dbReference type="HOGENOM" id="CLU_443879_0_0_2"/>
    <dbReference type="OrthoDB" id="94168at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009269">
        <property type="term" value="P:response to desiccation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008969">
        <property type="entry name" value="CarboxyPept-like_regulatory"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045043">
        <property type="entry name" value="Lea14-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004864">
        <property type="entry name" value="LEA_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013990">
        <property type="entry name" value="WHy-dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31459">
        <property type="entry name" value="PTHR31459"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03168">
        <property type="entry name" value="LEA_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00769">
        <property type="entry name" value="WHy"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49464">
        <property type="entry name" value="SSF49464"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="WHy" evidence="2">
        <location>
            <begin position="34"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="domain" description="WHy" evidence="2">
        <location>
            <begin position="185"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="domain" description="WHy" evidence="2">
        <location>
            <begin position="341"/>
            <end position="464"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF97118.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="615" mass="69045" checksum="EFB5A4E58E03DCFA" modified="2011-07-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBD3</accession>
    <name>F6BBD3_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="6">ZPR1-like zinc finger protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Metig_1607</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ZPR1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97140.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799733.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1607"/>
    <dbReference type="EnsemblBacteria" id="AEF97140">
        <property type="protein sequence ID" value="AEF97140"/>
        <property type="gene ID" value="Metig_1607"/>
    </dbReference>
    <dbReference type="GeneID" id="10644480"/>
    <dbReference type="KEGG" id="mig:Metig_1607"/>
    <dbReference type="HOGENOM" id="CLU_107446_0_0_2"/>
    <dbReference type="OMA" id="HSAIIGD"/>
    <dbReference type="OrthoDB" id="109705at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.1040">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004457">
        <property type="entry name" value="Znf_ZPR1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004470">
        <property type="entry name" value="Znf_ZPR1-type_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040141">
        <property type="entry name" value="ZPR1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042451">
        <property type="entry name" value="ZPR1_A/B_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042452">
        <property type="entry name" value="ZPR1_Znf1/2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10876">
        <property type="entry name" value="PTHR10876"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03367">
        <property type="entry name" value="zf-ZPR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00709">
        <property type="entry name" value="Zpr1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00340">
        <property type="entry name" value="zpr1_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00310">
        <property type="entry name" value="ZPR1_znf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Zpr1" evidence="5">
        <location>
            <begin position="10"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEF97140.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="195" mass="22223" checksum="30C9348ABAABCB53" modified="2011-07-27" version="1">MSKELAVQKLDCPICGGKNSFTITNHEIEIPYFGRVIETTMLCDRCKYRKSDVFPLEVREPKRYKLVIKNERDLNRRVIKSSTGYVKIPELGVEITPGPVSEGYVSNVEGVLNRVDNVLQTLIRWAENEEQKKKGEELRERIKKIKEGKETATLIIEDPLGHSAIIGDGVVEEKLTDEEIEKLKGNVIIIDTKKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBD4</accession>
    <name>F6BBD4_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Helix-turn-helix domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1608</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97141.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799734.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1608"/>
    <dbReference type="EnsemblBacteria" id="AEF97141">
        <property type="protein sequence ID" value="AEF97141"/>
        <property type="gene ID" value="Metig_1608"/>
    </dbReference>
    <dbReference type="GeneID" id="10644481"/>
    <dbReference type="KEGG" id="mig:Metig_1608"/>
    <dbReference type="HOGENOM" id="CLU_1297489_0_0_2"/>
    <dbReference type="OMA" id="PIYSIKK"/>
    <dbReference type="OrthoDB" id="63955at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01381">
        <property type="entry name" value="HTH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00530">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH cro/C1-type" evidence="1">
        <location>
            <begin position="19"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF97141.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="217" mass="25066" checksum="7F283CF147162644" modified="2011-07-27" version="1">MYKKLNIIERAILLNPKYVKIFREKLNITQSKLAEESGVSQSHLSMLEKGKRKATEMIAAAITLGLLKCSDVWDKEDPIMYLLDILSLTKLEEAIVEFINDINNSGNSPSFKRFVRYIEGYPVIVVDKDAFTEELKKRLDVIEIKNIEFKRGCMTVKGLHSDKREVVIDLDCSDIRRLEKKVSEKINKKTIIQIFPKDETPPIYSVNRDCMIVHCWG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBE1</accession>
    <name>F6BBE1_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1615</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97148.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799741.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1615"/>
    <dbReference type="EnsemblBacteria" id="AEF97148">
        <property type="protein sequence ID" value="AEF97148"/>
        <property type="gene ID" value="Metig_1615"/>
    </dbReference>
    <dbReference type="GeneID" id="10644488"/>
    <dbReference type="KEGG" id="mig:Metig_1615"/>
    <dbReference type="HOGENOM" id="CLU_1369520_0_0_2"/>
    <dbReference type="OMA" id="NMILLTD"/>
    <dbReference type="OrthoDB" id="83842at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019212">
        <property type="entry name" value="DUF2105_membrane"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09878">
        <property type="entry name" value="DUF2105"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="35"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="62"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="125"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="175"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="202"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF97148.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="229" mass="25006" checksum="E11C31203B051688" modified="2011-07-27" version="1">MMENIIYSFYNPSILVAFIVGILSLFALSYQKSDLHILLLTDLIECAMLVVIAAVGTDLAEALILPGLVVGMAELLAVSEILVMKKSLAKNKPKKKLFEEFLLPLHHGVLEHEVEMEILKTSPKFLALVLVVYGAILSGFTGGAIIASGLLYYALSQRALGREFDETIKCLWEGISGLSGIAWALWIFGFIGFFINPDNYLVWLMLAGLGLVIKVGSKLGLIGYIGEVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBI5</accession>
    <name>F6BBI5_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_0438</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95994.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798603.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF95994">
        <property type="protein sequence ID" value="AEF95994"/>
        <property type="gene ID" value="Metig_0438"/>
    </dbReference>
    <dbReference type="GeneID" id="10643275"/>
    <dbReference type="KEGG" id="mig:Metig_0438"/>
    <dbReference type="HOGENOM" id="CLU_1418699_0_0_2"/>
    <dbReference type="OMA" id="TFFYCAN"/>
    <dbReference type="OrthoDB" id="135458at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF95994.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="191" mass="22169" checksum="FA9198D4B89DEA6F" modified="2011-07-27" version="1">MNYEEIREKYNPTRQNIKVKVLFIGESPPKSTFFYCANSKLYSATKEAFEKAYGRKIPNSLQCFMLLGCYLIDLYNEPGRKAKKLCGLEVYEKEKEELIQRLASQIAQFNPHFIIAVHENVCSWSIISAIEALEILKERDKTPNFNLNAVRCLPFPKNCERGKQAYVGALVEIIKEMMQKNILPRELPKMH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBJ2</accession>
    <name>F6BBJ2_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Methyl-coenzyme M reductase subunit alpha</fullName>
            <ecNumber evidence="11">2.8.4.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ordered locus">Metig_0445</name>
    </gene>
    <organism evidence="17">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 11">Component of the methyl-coenzyme M reductase (MCR) I that catalyzes the reductive cleavage of methyl-coenzyme M (CoM-S-CH3 or 2-(methylthio)ethanesulfonate) using coenzyme B (CoB or 7-mercaptoheptanoylthreonine phosphate) as reductant which results in the production of methane and the mixed heterodisulfide of CoB and CoM (CoM-S-S-CoB). This is the final step in methanogenesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>coenzyme B + methyl-coenzyme M = coenzyme M-coenzyme B heterodisulfide + methane</text>
            <dbReference type="Rhea" id="RHEA:12532"/>
            <dbReference type="ChEBI" id="CHEBI:16183"/>
            <dbReference type="ChEBI" id="CHEBI:58286"/>
            <dbReference type="ChEBI" id="CHEBI:58411"/>
            <dbReference type="ChEBI" id="CHEBI:58596"/>
            <dbReference type="EC" id="2.8.4.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="1">
            <dbReference type="Rhea" id="RHEA:12533"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>coenzyme F430</name>
            <dbReference type="ChEBI" id="CHEBI:60540"/>
        </cofactor>
        <text evidence="11">Binds 2 coenzyme F430 non-covalently per MCR complex. Coenzyme F430 is a yellow nickel porphinoid. Methyl-coenzyme-M reductase is activated when the enzyme-bound coenzyme F430 is reduced to the Ni(I) oxidation state.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3 11">One-carbon metabolism; methyl-coenzyme M reduction; methane from methyl-coenzyme M: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Hexamer of two alpha, two beta, and two gamma chains.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">MCR is a hexamer of two alpha, two beta, and two gamma chains, forming a dimer of heterotrimers.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the methyl-coenzyme M reductase alpha subunit family.</text>
    </comment>
    <dbReference type="EC" id="2.8.4.1" evidence="11"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96001.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798610.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0445"/>
    <dbReference type="EnsemblBacteria" id="AEF96001">
        <property type="protein sequence ID" value="AEF96001"/>
        <property type="gene ID" value="Metig_0445"/>
    </dbReference>
    <dbReference type="GeneID" id="10643282"/>
    <dbReference type="KEGG" id="mig:Metig_0445"/>
    <dbReference type="HOGENOM" id="CLU_493170_0_0_2"/>
    <dbReference type="OMA" id="VGHQPEY"/>
    <dbReference type="OrthoDB" id="6589at2157"/>
    <dbReference type="UniPathway" id="UPA00646">
        <property type="reaction ID" value="UER00699"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050524">
        <property type="term" value="F:coenzyme-B sulfoethylthiotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015948">
        <property type="term" value="P:methanogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.840.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.470">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.390.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016212">
        <property type="entry name" value="Me_CoM_Rdtase_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008924">
        <property type="entry name" value="Me_CoM_Rdtase_asu/bsu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009047">
        <property type="entry name" value="Me_CoM_Rdtase_asu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003183">
        <property type="entry name" value="Me_CoM_Rdtase_asu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015811">
        <property type="entry name" value="Me_CoM_Rdtase_asu_N_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015823">
        <property type="entry name" value="Me_CoM_Rdtase_asu_N_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009024">
        <property type="entry name" value="Me_CoM_Rdtase_Fd-like_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02249">
        <property type="entry name" value="MCR_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02745">
        <property type="entry name" value="MCR_alpha_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000262">
        <property type="entry name" value="MCR_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48081">
        <property type="entry name" value="SSF48081"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55088">
        <property type="entry name" value="SSF55088"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03256">
        <property type="entry name" value="met_CoM_red_alp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 11 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 11" id="KW-0484">Methanogenesis</keyword>
    <keyword evidence="6" id="KW-0488">Methylation</keyword>
    <keyword evidence="7 11 12" id="KW-0533">Nickel</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 11 16" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="MCR_alpha_N" evidence="15">
        <location>
            <begin position="5"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="domain" description="MCR_alpha" evidence="14">
        <location>
            <begin position="317"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="149"/>
        </location>
        <ligand>
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="Pros-methylhistidine" evidence="13">
        <location>
            <position position="259"/>
        </location>
    </feature>
    <feature type="modified residue" description="5-methylarginine" evidence="13">
        <location>
            <position position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000951"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR000262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR000262-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR000262-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF02249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF02745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="AEF96001.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="551" mass="61218" checksum="70FC3B771CDEDE40" modified="2011-07-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBL2</accession>
    <name>F6BBL2_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Thioredoxin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Metig_0465</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Does not function as a glutathione-disulfide oxidoreductase in the presence of glutathione and glutathione reductase. Has low thioredoxin activity in vitro.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the glutaredoxin family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96021.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798629.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0465"/>
    <dbReference type="EnsemblBacteria" id="AEF96021">
        <property type="protein sequence ID" value="AEF96021"/>
        <property type="gene ID" value="Metig_0465"/>
    </dbReference>
    <dbReference type="GeneID" id="10643302"/>
    <dbReference type="KEGG" id="mig:Metig_0465"/>
    <dbReference type="HOGENOM" id="CLU_090389_18_1_2"/>
    <dbReference type="OMA" id="KYGVMST"/>
    <dbReference type="OrthoDB" id="114780at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005243">
        <property type="entry name" value="Redox_disulphide_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012336">
        <property type="entry name" value="Thioredoxin-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36450">
        <property type="entry name" value="PTHR36450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13192">
        <property type="entry name" value="Thioredoxin_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037031">
        <property type="entry name" value="Redox_disulphide_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00412">
        <property type="entry name" value="redox_disulf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2 3" id="KW-0249">Electron transport</keyword>
    <keyword evidence="3 5" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Thioredoxin-like_fold" evidence="6">
        <location>
            <begin position="1"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="4">
        <location>
            <position position="10"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="4">
        <location>
            <position position="13"/>
        </location>
    </feature>
    <feature type="disulfide bond" description="Redox-active" evidence="5">
        <location>
            <begin position="10"/>
            <end position="13"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRNR" id="PIRNR037031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR037031-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR037031-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF13192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEF96021.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="80" mass="8920" checksum="DD1E7946BDAE5D42" modified="2011-07-27" version="1">MIIRVFGTGCPKCNQTYENVKKAVEELGIDAEIVKVTDINEISEWIFVAPGVAFDDEIVFEGKVPTVEEIKKELKDYLGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBM1</accession>
    <name>F6BBM1_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">UbiD family decarboxylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_0474</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96030.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798638.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0474"/>
    <dbReference type="EnsemblBacteria" id="AEF96030">
        <property type="protein sequence ID" value="AEF96030"/>
        <property type="gene ID" value="Metig_0474"/>
    </dbReference>
    <dbReference type="GeneID" id="10643311"/>
    <dbReference type="KEGG" id="mig:Metig_0474"/>
    <dbReference type="HOGENOM" id="CLU_023348_5_1_2"/>
    <dbReference type="OMA" id="TEGGCCW"/>
    <dbReference type="OrthoDB" id="35356at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016831">
        <property type="term" value="F:carboxy-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002830">
        <property type="entry name" value="UbiD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30108">
        <property type="entry name" value="PTHR30108"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01977">
        <property type="entry name" value="UbiD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00148">
        <property type="entry name" value="TIGR00148"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF96030.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="422" mass="47398" checksum="B4F13375B5CD7896" modified="2011-07-27" version="1">MLRELINEINPIVVEKASKKFGVSRILKKYDGQPVYIKDVDGYEVIGNLCNREVLAKSLGIKKEDLMMHILKAMDNEKEGKLVVDKSLKNKYVEDDLDKLKEYPIPTYYEKDAGPYITSGVVIVKDEDFGYNASIHRILVKDEHLVIRMVEQRHLHYLYTKNMKEKDEMDVAIVIGVHPAVLLAASTSADITFDELRFASALMGEPLKVMECDNGLLVPEGEFIIEGKITKDMEDEGPFVDITGTYDIVRKQPVIKITSLKRKEKPIFHALLPGGKEHRLLMGMPQEPRIFKGVRNVVPTVKNVVLTEGGCCWLHAVVQIEKKTEGDGKNAILAALSSHPSLKHVVVVDEDIDIYDINDVEFAIATRVQGDEDIIIIRGAKGSSLDPSSNLEYKTTTKIGIDATISFKKDKSHFIRAKIPDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBS9</accession>
    <name>F6BBS9_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_1677</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97209.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799800.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1677"/>
    <dbReference type="EnsemblBacteria" id="AEF97209">
        <property type="protein sequence ID" value="AEF97209"/>
        <property type="gene ID" value="Metig_1677"/>
    </dbReference>
    <dbReference type="GeneID" id="10644550"/>
    <dbReference type="KEGG" id="mig:Metig_1677"/>
    <dbReference type="HOGENOM" id="CLU_128028_0_0_2"/>
    <dbReference type="OMA" id="QVEINSE"/>
    <dbReference type="OrthoDB" id="79095at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF97209.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="174" mass="20225" checksum="00BFFBA78477B5F6" modified="2011-07-27" version="1">MTYLVVVDYEKDSERKRIDYLIERWSNRGSIEKIRKMAIIVDVDNIDDFIKDIMSRLEGNPEEKVKVYEIKEVKKTIPSKKITLNYEVADKKEVVEGFLKYLMAKIGASYECSINDTKKYEAYTKKGSCSISIKLKEKGNKLIINFEIEGYGESVDLIKNKIDNDMKLFIEGLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-06-02" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBZ5</accession>
    <name>F6BBZ5_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_0521</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96076.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96076">
        <property type="protein sequence ID" value="AEF96076"/>
        <property type="gene ID" value="Metig_0521"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0521"/>
    <dbReference type="HOGENOM" id="CLU_194995_0_0_2"/>
    <dbReference type="OMA" id="YPTFSGV"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019205">
        <property type="entry name" value="DUF2080_transposon-encoded"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09853">
        <property type="entry name" value="DUF2080"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF96076.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="79" mass="8840" checksum="1C9439F0C66DB325" modified="2011-07-27" version="1">MVVIIILFVKICSNSDSMPEIKKKRWAKARIIFPAIYGKIKEQGNSARFFPSIPAEYVGKKAVLVILEEDKELDGGGDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBZ7</accession>
    <name>F6BBZ7_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DNA polymerase sliding clamp</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Proliferating cell nuclear antigen homolog</fullName>
            <shortName evidence="3">PCNA</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">pcn</name>
        <name evidence="8" type="ordered locus">Metig_0523</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Sliding clamp subunit that acts as a moving platform for DNA processing. Responsible for tethering the catalytic subunit of DNA polymerase and other proteins to DNA during high-speed replication.</text>
    </comment>
    <comment type="function">
        <text evidence="5">Sliding clamp subunit. Responsible for tethering the catalytic subunit of DNA polymerase to DNA during high-speed replication.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homotrimer. The subunits circularize to form a toroid; DNA passes through its center. Replication factor C (RFC) is required to load the toroid on the DNA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 3 4">Belongs to the PCNA family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96078.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798686.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0523"/>
    <dbReference type="EnsemblBacteria" id="AEF96078">
        <property type="protein sequence ID" value="AEF96078"/>
        <property type="gene ID" value="Metig_0523"/>
    </dbReference>
    <dbReference type="GeneID" id="10643360"/>
    <dbReference type="KEGG" id="mig:Metig_0523"/>
    <dbReference type="HOGENOM" id="CLU_043978_1_1_2"/>
    <dbReference type="OMA" id="EMKLINM"/>
    <dbReference type="OrthoDB" id="70433at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030337">
        <property type="term" value="F:DNA polymerase processivity factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006275">
        <property type="term" value="P:regulation of DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00317">
        <property type="entry name" value="DNApol_clamp_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000730">
        <property type="entry name" value="Pr_cel_nuc_antig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022649">
        <property type="entry name" value="Pr_cel_nuc_antig_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022648">
        <property type="entry name" value="Pr_cel_nuc_antig_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11352:SF0">
        <property type="entry name" value="PTHR11352:SF0"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02747">
        <property type="entry name" value="PCNA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00705">
        <property type="entry name" value="PCNA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00339">
        <property type="entry name" value="PCNACYCLIN"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00590">
        <property type="entry name" value="pcna"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0235">DNA replication</keyword>
    <keyword evidence="2 3 4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PCNA_N" evidence="6">
        <location>
            <begin position="1"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="domain" description="PCNA_C" evidence="7">
        <location>
            <begin position="124"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEF96078.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="247" mass="27406" checksum="AE9960DBD5B2F25C" modified="2011-07-27" version="1">MFKAVISGAKNFKKVIDAASNLLIEICFEVNEERVKAIAMDPDHVALVSIDIPKEAFDEYEADVHSIGVDLEAFKKIMNMAKAKDKLILELDEEKDKLNVVFKGAMTRKFALALYDVSSLDLQVPDIEYPNEILIKAGPFVDALKGANLVSDWATLKVEDGKFIVYAKGDLNESETVFEEDSESIISMNIGEDAKSTFYLEYLRDMTKAASSGDILKIYLGSDMPVKVEYDVAGANLTFLLAPRIES</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-09-29" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>F6BC12</accession>
    <name>F6BC12_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_0538</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96093.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798700.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96093">
        <property type="protein sequence ID" value="AEF96093"/>
        <property type="gene ID" value="Metig_0538"/>
    </dbReference>
    <dbReference type="GeneID" id="10643376"/>
    <dbReference type="KEGG" id="mig:Metig_0538"/>
    <dbReference type="HOGENOM" id="CLU_638758_0_0_2"/>
    <dbReference type="OMA" id="IPNFRYW"/>
    <dbReference type="OrthoDB" id="91618at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF96093.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="408" mass="48826" checksum="C81F5ED440FDA76F" modified="2011-07-27" version="1">MKSYYDTIPNFKYWDVEAWKEYIQKYIVPIYENTKYLLELKDELSLYVGKTLETLRENNKDLMPFFVGGIDEEGNYEESSIARLIYLMFGIYIEPKVFIGEGRLKEAIITSKNEDEWTFLRFLKEMNECSLNIIKKSEIKLSSIKIDDTIQNPSKILEILNELYKGCVSFNASYNYYTFFIVSAMGVHWKYLISTYPKLNEYDGIKEFLGFHPMFVPDAEDEFKKDYTIWRYKNEGIASNLFAMQLYLWYLFDFINYDDYKEIFGYVIEDPKNLREEFRKLVKTTLGCEGEITIKNIKINPKDVYLVAIEKEYIIKWPKKEVVDRFYDAFERRWKLSHYFRREYEIPFEEERSKPKILLSDFLDEVAPFLFLKLAYFSLSMFFLPDPTCADNPLKSPHNKLIYSHICG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-06-02" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>F6BC19</accession>
    <name>F6BC19_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0546</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96100.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798707.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96100">
        <property type="protein sequence ID" value="AEF96100"/>
        <property type="gene ID" value="Metig_0546"/>
    </dbReference>
    <dbReference type="GeneID" id="10643384"/>
    <dbReference type="KEGG" id="mig:Metig_0546"/>
    <dbReference type="HOGENOM" id="CLU_968437_0_0_2"/>
    <dbReference type="OMA" id="EEDFCIG"/>
    <dbReference type="OrthoDB" id="104760at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="249"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96100.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="272" mass="30929" checksum="674DB095B986EC86" modified="2011-07-27" version="1">MNKRFLVLLTLISIFLDISYAEDIYVKLVPEKVVSNGEIIKFNITVENIPKIGEIKDEYNGMEDGGCRGVSICINYSDYLKPYGFNWSDICKKEKLKSYEFKDGVFNAKVVFDKPIEEDFCIGEVMFIPIKEGEVQLNLSGSVSSTLGYKYDGVNEYYDYYTKTFKKYPNTKFLGANITIKSAGNVTNTTMKLKEDVEEYESSSSSTIINRIYITPNIKEPEVIVKEINLTEYQPIVNVVVEKKGSFKFLGFNLLSGILAGILFGFILSKFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>F6BC29</accession>
    <name>F6BC29_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein Metig_0556</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_0556</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96110.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798717.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0556"/>
    <dbReference type="EnsemblBacteria" id="AEF96110">
        <property type="protein sequence ID" value="AEF96110"/>
        <property type="gene ID" value="Metig_0556"/>
    </dbReference>
    <dbReference type="GeneID" id="10643394"/>
    <dbReference type="KEGG" id="mig:Metig_0556"/>
    <dbReference type="HOGENOM" id="CLU_095686_1_1_2"/>
    <dbReference type="OMA" id="RGCIGFP"/>
    <dbReference type="OrthoDB" id="107157at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.700.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00645">
        <property type="entry name" value="AMMECR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023473">
        <property type="entry name" value="AMMECR1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036071">
        <property type="entry name" value="AMMECR1_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002733">
        <property type="entry name" value="AMMECR1_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027485">
        <property type="entry name" value="AMMECR1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027623">
        <property type="entry name" value="AmmeMemoSam_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023472">
        <property type="entry name" value="Uncharacterised_MJ0810"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13016">
        <property type="entry name" value="PTHR13016"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01871">
        <property type="entry name" value="AMMECR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143447">
        <property type="entry name" value="SSF143447"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR04335">
        <property type="entry name" value="AmmeMemoSam_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00296">
        <property type="entry name" value="TIGR00296"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51112">
        <property type="entry name" value="AMMECR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMMECR1" evidence="2">
        <location>
            <begin position="6"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF96110.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="202" mass="22975" checksum="FA0584EFE06F9F9E" modified="2011-07-27" version="1">MKLTLEEGIFAVKFARNVIESHLSGRDLVVNNYPPKFNEKRGAFVTLHTYPGHDLRGCIGIPEPIMPLIEALKEAAISSAISDPRFPPVTLEEMDNIVVEVSILTAPQLIRVSHPREYLEKIKIGRDGLIIEYGVYRGLLLPQVPVEYNWDVEEYLANLCLKAGLPPDMWLEEGVKIYRFEAQIFEEVEPRGNIVEKITYVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>F6BC91</accession>
    <name>F6BC91_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Orotidine 5'-phosphate decarboxylase</fullName>
            <ecNumber evidence="5">4.1.1.23</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">OMP decarboxylase</fullName>
            <shortName evidence="5">OMPDCase</shortName>
            <shortName evidence="5">OMPdecase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">pyrF</name>
        <name evidence="8" type="ordered locus">Metig_1765</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the decarboxylation of orotidine 5'-monophosphate (OMP) to uridine 5'-monophosphate (UMP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5 6">
            <text>H(+) + orotidine 5'-phosphate = CO2 + UMP</text>
            <dbReference type="Rhea" id="RHEA:11596"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57538"/>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
            <dbReference type="EC" id="4.1.1.23"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 5 6">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from orotate: step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the OMP decarboxylase family. Type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.23" evidence="5"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97297.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799886.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1765"/>
    <dbReference type="EnsemblBacteria" id="AEF97297">
        <property type="protein sequence ID" value="AEF97297"/>
        <property type="gene ID" value="Metig_1765"/>
    </dbReference>
    <dbReference type="GeneID" id="10644639"/>
    <dbReference type="KEGG" id="mig:Metig_1765"/>
    <dbReference type="HOGENOM" id="CLU_067069_2_0_2"/>
    <dbReference type="OMA" id="EMSHPGA"/>
    <dbReference type="OrthoDB" id="54384at2157"/>
    <dbReference type="UniPathway" id="UPA00070">
        <property type="reaction ID" value="UER00120"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004590">
        <property type="term" value="F:orotidine-5'-phosphate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006207">
        <property type="term" value="P:'de novo' pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044205">
        <property type="term" value="P:'de novo' UMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01200_A">
        <property type="entry name" value="OMPdecase_type1_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014732">
        <property type="entry name" value="OMPdecase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018089">
        <property type="entry name" value="OMPdecase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001754">
        <property type="entry name" value="OMPdeCOase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32119:SF2">
        <property type="entry name" value="PTHR32119:SF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00215">
        <property type="entry name" value="OMPdecase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00934">
        <property type="entry name" value="OMPdecase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="SSF51366"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01740">
        <property type="entry name" value="pyrF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00156">
        <property type="entry name" value="OMPDECASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5 6" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="4 5 6" id="KW-0456">Lyase</keyword>
    <keyword evidence="3 5 6" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="OMPdecase" evidence="7">
        <location>
            <begin position="3"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="5">
        <location>
            <begin position="59"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="5">
        <location>
            <begin position="167"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="5">
        <location>
            <position position="61"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="9"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="31"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="115"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="192"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="193"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEF97297.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="216" mass="23881" checksum="32AA19020B5E3DEB" modified="2011-07-27" version="1">MVKLMLALDVMDKDKALKIARETSEYVDAIKIGYPLVLATNLKIVKEIKELSNKEVICDFKVADIPSTNEKIAKLTLQYADGIICHGFVGEDSIRAIQNVAKENNKKVIMVTEMSHPGAVMFMQPIANELAKLAKKLGVDGVVAPSTRPERLKEIKEIVGEIPIVSPGIGAQGGDLEKVLEILSDNDYIIVGRSIYNSENPKESAKMYKEKLKNRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>F6BC95</accession>
    <name>F6BC95_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Mannose-1-phosphate guanylyltransferase</fullName>
            <ecNumber evidence="3">2.7.7.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Metig_1769</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>alpha-D-mannose 1-phosphate + GTP + H(+) = diphosphate + GDP-alpha-D-mannose</text>
            <dbReference type="Rhea" id="RHEA:15229"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:57527"/>
            <dbReference type="ChEBI" id="CHEBI:58409"/>
            <dbReference type="EC" id="2.7.7.13"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the mannose-6-phosphate isomerase type 2 family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.13" evidence="3"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97301.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799890.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1769"/>
    <dbReference type="EnsemblBacteria" id="AEF97301">
        <property type="protein sequence ID" value="AEF97301"/>
        <property type="gene ID" value="Metig_1769"/>
    </dbReference>
    <dbReference type="GeneID" id="10644643"/>
    <dbReference type="KEGG" id="mig:Metig_1769"/>
    <dbReference type="HOGENOM" id="CLU_035527_1_0_2"/>
    <dbReference type="OMA" id="LIVCNEK"/>
    <dbReference type="OrthoDB" id="33376at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004475">
        <property type="term" value="F:mannose-1-phosphate guanylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000271">
        <property type="term" value="P:polysaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006375">
        <property type="entry name" value="Man1P_GuaTrfase/Man6P_Isoase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001538">
        <property type="entry name" value="Man6P_isomerase-2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005835">
        <property type="entry name" value="NTP_transferase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01050">
        <property type="entry name" value="MannoseP_isomer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00483">
        <property type="entry name" value="NTP_transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="SSF51182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01479">
        <property type="entry name" value="GMP_PMI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4 8" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="NTP_transferase" evidence="6">
        <location>
            <begin position="2"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="domain" description="MannoseP_isomer" evidence="7">
        <location>
            <begin position="297"/>
            <end position="445"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU004190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEF97301.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="449" mass="52291" checksum="5CA2D143FC1915C1" modified="2011-07-27" version="1">MKSVILAGGSGTRLFPLSREYFPKQFIKLRGFDKSLFQMTLDRALNLTKPKDILIVTNERHKFLVMNEVENYNIPENNILLEPEGKNTLPAIYYAVKDLNDIVAVFPSDHLILEEDELIETIKEGQKLTKNYLITFGVKPNKPHTGYGYIKPGEKLEIGYKADEFKEKPNLDLAIEYIKNGYLWNSGMFLFDSELFKEEVKKYCPEVYEAFKLNDIKDIYELAPNISIDYGVLEKSNKVAVIPLNITWSDLGSFDALYDIFDKDENGNILNEDVKYINSKNNFVYSSSKKLITLLDINDLIVVDTKDSLVICKRGSSEKIKDLVKTLKKENDERILYHKKVFRPWGYYEVLEEGMFYKVKKITVYPKKRLSLQMHYHRSEHWIVVKGMAKVVIEGKEFFVRPGESVFVKSGEKHRLENPGKIPLEVIEIQVGEYLEEDDIVRFDDDWRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCA4</accession>
    <name>F6BCA4_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA primase DnaG</fullName>
            <ecNumber evidence="1">2.7.7.101</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">dnaG</name>
        <name evidence="3" type="ordered locus">Metig_1778</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">RNA polymerase that catalyzes the synthesis of short RNA molecules used as primers for DNA polymerase during DNA replication.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Forms a ternary complex with MCM helicase and DNA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the archaeal DnaG primase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.101" evidence="1"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97310.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799899.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1778"/>
    <dbReference type="EnsemblBacteria" id="AEF97310">
        <property type="protein sequence ID" value="AEF97310"/>
        <property type="gene ID" value="Metig_1778"/>
    </dbReference>
    <dbReference type="GeneID" id="10644652"/>
    <dbReference type="KEGG" id="mig:Metig_1778"/>
    <dbReference type="HOGENOM" id="CLU_034626_0_0_2"/>
    <dbReference type="OMA" id="KYMIVAQ"/>
    <dbReference type="OrthoDB" id="23182at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000178">
        <property type="term" value="C:exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990077">
        <property type="term" value="C:primosome complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003896">
        <property type="term" value="F:DNA primase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008143">
        <property type="term" value="F:poly(A) binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01029">
        <property type="entry name" value="TOPRIM_primases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00007">
        <property type="entry name" value="DNA_primase_DnaG_arc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020607">
        <property type="entry name" value="Primase_DnaG_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034154">
        <property type="entry name" value="TOPRIM_DnaG/twinkle"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006171">
        <property type="entry name" value="TOPRIM_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13662">
        <property type="entry name" value="Toprim_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00493">
        <property type="entry name" value="TOPRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50880">
        <property type="entry name" value="TOPRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0235">DNA replication</keyword>
    <keyword evidence="1" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="1" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="1" id="KW-0639">Primosome</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Toprim" evidence="2">
        <location>
            <begin position="168"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF97310.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="423" mass="46839" checksum="C030D87B15F2C54B" modified="2011-07-27" version="1">MDLGTTKYIIHAELIADGYVEKHDVIGAIFGQTEGLLGDELDLRELQKSGRIGRIDVELENVNGKSIAKITVPSSLDRVETSILAATLEAIDRVGPCVATVRVINIEDIRATKRQYIINRAKEILRSLVNSIDVHEITEAVKESVRMEEVIEYGEDKLPAGPGILNSDSIIVVEGRADVLNLLRCGIKNVIAVEGTSVPKTVVELTKKKITTVFTDGDRGGELILKELLQTCDIDYVARAPNGKEVEELSKKEIIKCLRAKVPAGQVKMQLFNNNTKNGTTNGKVIIADAIKHDENNKNKIVNKDLDDIIVKSTVEVAPDIKNNVEESTELANVNNDVPYLDIIKDIIGTNKVKVIINGNEEIKEFEEFMNEINDIEKIDAVILDMPITQKIVDNLYEKTDLIIGRDIKVTKKPTNLRLLSFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-06-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCC9</accession>
    <name>F6BCC9_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_0586</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96140.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798747.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96140">
        <property type="protein sequence ID" value="AEF96140"/>
        <property type="gene ID" value="Metig_0586"/>
    </dbReference>
    <dbReference type="GeneID" id="10643424"/>
    <dbReference type="KEGG" id="mig:Metig_0586"/>
    <dbReference type="HOGENOM" id="CLU_2476019_0_0_2"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF96140.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="87" mass="10231" checksum="0439A14B2C2C036C" modified="2011-07-27" version="1">MLIKSKHDKFIKLIKTAKSHDKLKNDISYEIITNNGKAIIKIPSKEALIWFIRVCNKEVLTDLLQEEIYIVEKKTPYQTTLDLWIQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCK0</accession>
    <name>F6BCK0_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Flap endonuclease 1</fullName>
            <shortName evidence="11">FEN-1</shortName>
            <ecNumber evidence="11">3.1.-.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Flap structure-specific endonuclease 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">fen</name>
        <name evidence="14" type="ordered locus">Metig_0661</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. Binds the unpaired 3'-DNA end and kinks the DNA to facilitate 5' cleavage specificity. Cleaves one nucleotide into the double-stranded DNA from the junction in flap DNA, leaving a nick for ligation. Also involved in the base excision repair (BER) pathway. Acts as a genome stabilization factor that prevents flaps from equilibrating into structures that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA.</text>
    </comment>
    <comment type="function">
        <text evidence="11">Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. Binds the unpaired 3'-DNA end and kinks the DNA to facilitate 5' cleavage specificity. Cleaves one nucleotide into the double-stranded DNA from the junction in flap DNA, leaving a nick for ligation. Also involved in the base excision repair (BER) pathway. Acts as a genome stabilization factor that prevents flaps from equilibrating into structurs that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="11">Binds 2 magnesium ions per subunit. They probably participate in the reaction catalyzed by the enzyme. May bind an additional third magnesium ion after substrate binding.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Interacts with PCNA. PCNA stimulates the nuclease activity without altering cleavage specificity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the XPG/RAD2 endonuclease family. FEN1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="11"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96211.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798814.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0661"/>
    <dbReference type="EnsemblBacteria" id="AEF96211">
        <property type="protein sequence ID" value="AEF96211"/>
        <property type="gene ID" value="Metig_0661"/>
    </dbReference>
    <dbReference type="GeneID" id="10643501"/>
    <dbReference type="KEGG" id="mig:Metig_0661"/>
    <dbReference type="HOGENOM" id="CLU_032444_0_0_2"/>
    <dbReference type="OMA" id="GSQDYDS"/>
    <dbReference type="OrthoDB" id="41214at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008409">
        <property type="term" value="F:5'-3' exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017108">
        <property type="term" value="F:5'-flap endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043137">
        <property type="term" value="P:DNA replication, removal of RNA primer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00614">
        <property type="entry name" value="Fen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036279">
        <property type="entry name" value="5-3_exonuclease_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023426">
        <property type="entry name" value="Flap_endonuc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019973">
        <property type="entry name" value="Flap_structure-sp_endonuc_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008918">
        <property type="entry name" value="HhH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006086">
        <property type="entry name" value="XPG-I_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006084">
        <property type="entry name" value="XPG/Rad2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006085">
        <property type="entry name" value="XPG_DNA_repair_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11081">
        <property type="entry name" value="PTHR11081"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00867">
        <property type="entry name" value="XPG_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00752">
        <property type="entry name" value="XPG_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00853">
        <property type="entry name" value="XPGRADSUPER"/>
    </dbReference>
    <dbReference type="SMART" id="SM00279">
        <property type="entry name" value="HhH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00484">
        <property type="entry name" value="XPGI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00485">
        <property type="entry name" value="XPGN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47807">
        <property type="entry name" value="SSF47807"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="SSF88723"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03674">
        <property type="entry name" value="fen_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 11" id="KW-0227">DNA damage</keyword>
    <keyword evidence="9 11" id="KW-0234">DNA repair</keyword>
    <keyword evidence="1 11" id="KW-0235">DNA replication</keyword>
    <keyword evidence="4 11 14" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="7 11" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="6 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8 11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 11" id="KW-0540">Nuclease</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="XPGN" evidence="13">
        <location>
            <begin position="1"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="domain" description="XPGI" evidence="12">
        <location>
            <begin position="140"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="region of interest" description="N-domain" evidence="11">
        <location>
            <begin position="1"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="I-domain" evidence="11">
        <location>
            <begin position="116"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="region of interest" description="Interaction with PCNA" evidence="11">
        <location>
            <begin position="316"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="27"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="80"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="152"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="154"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="173"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="175"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="224"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00024702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="AEF96211.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="326" mass="37576" checksum="181E204F40F8A1AA" modified="2011-07-27" version="1">MGVQFNDLIPKKEIPIKYLSGKTVAIDGMNVLYQFLSSIRLRDGSPLRNRKGEITSTYNGIFYKTIYMLENDITPVWVFDGKPPKLKEKTREERRKMREKAKEEFTKAKEMENIDEMQKYAKRMNFLTKDIVENSKKLLDLMGVPYVNAPAEGEGQASYMAKKGDVFCVISQDYDALLYGAPRIVRNLTATKEELELIELENVLNELGISHDDLIDMAILIGTDYNPKGVKGIGPKKALEIVKSKNKELYLKAVENYEEIKNIFKNPKVTDEYSIKLKKPDKEGIIKFLVEENDFSMERVQPHVEKLCKLIEKKTKQVTLDAWFGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCK1</accession>
    <name>F6BCK1_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Probable dihydroorotate dehydrogenase B (NAD(+)), electron transfer subunit</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Dihydroorotate oxidase B, electron transfer subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">pyrK</name>
        <name evidence="8" type="ordered locus">Metig_0662</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Responsible for channeling the electrons from the oxidation of dihydroorotate from the FMN redox center in the PyrD type B subunit to the ultimate electron acceptor NAD(+).</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5 6">
            <name>2Fe-2S cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49601"/>
        </cofactor>
        <text evidence="5 6">Binds 1 [2Fe-2S] cluster per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
        <text evidence="5">Binds 1 FAD per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="5">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; orotate from (S)-dihydroorotate (NAD(+) route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Heterotetramer of 2 PyrK and 2 PyrD type B subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the PyrK family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96212.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0662"/>
    <dbReference type="EnsemblBacteria" id="AEF96212">
        <property type="protein sequence ID" value="AEF96212"/>
        <property type="gene ID" value="Metig_0662"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0662"/>
    <dbReference type="HOGENOM" id="CLU_003827_1_1_2"/>
    <dbReference type="OMA" id="RYMKCGI"/>
    <dbReference type="UniPathway" id="UPA00070">
        <property type="reaction ID" value="UER00945"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044205">
        <property type="term" value="P:'de novo' UMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.240.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01211">
        <property type="entry name" value="DHODB_Fe_S_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012165">
        <property type="entry name" value="Cyt_c3_hydrogenase_gsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037117">
        <property type="entry name" value="Dihydroorotate_DH_ele_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019480">
        <property type="entry name" value="Dihydroorotate_DH_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023455">
        <property type="entry name" value="Dihydroorotate_DHASE_ETsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017927">
        <property type="entry name" value="FAD-bd_FR_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001433">
        <property type="entry name" value="OxRdtase_FAD/NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10418">
        <property type="entry name" value="DHODB_Fe-S_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00175">
        <property type="entry name" value="NAD_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006816">
        <property type="entry name" value="Cyc3_hyd_g"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52343">
        <property type="entry name" value="SSF52343"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="SSF63380"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51384">
        <property type="entry name" value="FAD_FR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="3 5" id="KW-0249">Electron transport</keyword>
    <keyword evidence="5" id="KW-0274">FAD</keyword>
    <keyword evidence="5" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5 6" id="KW-0408">Iron</keyword>
    <keyword evidence="4 5 6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="5 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 5" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="FAD-binding FR-type" evidence="7">
        <location>
            <begin position="10"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5 6">
        <location>
            <position position="216"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5 6">
        <location>
            <position position="221"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5 6">
        <location>
            <position position="224"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5 6">
        <location>
            <position position="234"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR006816-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEF96212.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="266" mass="29422" checksum="4042677F07E5F6EE" modified="2011-07-27" version="1">MHGLGDENMERPVMCKIKEIIEESPTVKTFIIDKDFDFKPGQFAMVWLPGVDEKPFGFSTKTGFSIAKVGNFTAKMHELQEGDLIGVRGPYGTYFEPLGDRILAVAGGIGAAPIVAAVEEFAKQGIEITTIIGARTKEELLFLDRFENCGELEICTDDGSFGFHGFTTQKMSEILKEEKFDLVITCGPEIMMKKVVEIANEYNIPVQVSMERYMKCGIGICGQCCVDDEGLCVCKDGPVFWGDKLKFVTEFGKYKRDASGSIALNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCL5</accession>
    <name>F6BCL5_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uroporphyrinogen-III decarboxylase-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0676</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96226.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798829.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0676"/>
    <dbReference type="EnsemblBacteria" id="AEF96226">
        <property type="protein sequence ID" value="AEF96226"/>
        <property type="gene ID" value="Metig_0676"/>
    </dbReference>
    <dbReference type="GeneID" id="10643516"/>
    <dbReference type="KEGG" id="mig:Metig_0676"/>
    <dbReference type="HOGENOM" id="CLU_1040539_0_0_2"/>
    <dbReference type="OMA" id="FLHICGE"/>
    <dbReference type="OrthoDB" id="62744at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004853">
        <property type="term" value="F:uroporphyrinogen decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006779">
        <property type="term" value="P:porphyrin-containing compound biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038071">
        <property type="entry name" value="UROD/MetE-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000257">
        <property type="entry name" value="Uroporphyrinogen_deCOase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01208">
        <property type="entry name" value="URO-D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51726">
        <property type="entry name" value="SSF51726"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Uroporphyrinogen_deCOase" evidence="1">
        <location>
            <begin position="64"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96226.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="267" mass="30679" checksum="DC2ABC53687F740B" modified="2011-07-27" version="1">MTPKERIDSVLSGGGNVPSTFIASPIFLNRHIKDSKNLANFYLELYKKYRPDLITIIHENTYLEIQALKILRRKIKDTYITVSLNCPISYLSATDDNFMVNLYLKKEEIHSKIYNYVEFLKDNIRTIIENDGIPFLGDPTAPFIGPKMYKEFGIPYLKKIFKFIKSFGIPTFLHICGELSPIIEQLKNLDIDVLSFEDSLNVVGTLDCVLMGNVKPKDMLNGNIDSQIHYILKNRKKQHILSTGCELLPNTPEKNIIKLINSRVDYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-09-29" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCM4</accession>
    <name>F6BCM4_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0685</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96235.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96235">
        <property type="protein sequence ID" value="AEF96235"/>
        <property type="gene ID" value="Metig_0685"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0685"/>
    <dbReference type="HOGENOM" id="CLU_3003135_0_0_2"/>
    <dbReference type="OMA" id="KHTEEHL"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="28"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96235.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="58" mass="7026" checksum="FB9F73D161CA6227" modified="2011-07-27" version="1">MEDEKKIKKLLHLLEHTEEHFEIIINLMKELNLNAEGYEKLYDTLKNENEKLKKELSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCN8</accession>
    <name>F6BCN8_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="7">ABC-type transporter, integral membrane subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Metig_0699</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96249.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798852.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0699"/>
    <dbReference type="EnsemblBacteria" id="AEF96249">
        <property type="protein sequence ID" value="AEF96249"/>
        <property type="gene ID" value="Metig_0699"/>
    </dbReference>
    <dbReference type="GeneID" id="10643539"/>
    <dbReference type="KEGG" id="mig:Metig_0699"/>
    <dbReference type="HOGENOM" id="CLU_031365_1_2_2"/>
    <dbReference type="OMA" id="VFWYKLQ"/>
    <dbReference type="OrthoDB" id="49280at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015658">
        <property type="term" value="F:branched-chain amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06581">
        <property type="entry name" value="TM_PBP1_LivM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001851">
        <property type="entry name" value="ABC_transp_permease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043428">
        <property type="entry name" value="LivM-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30482">
        <property type="entry name" value="PTHR30482"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02653">
        <property type="entry name" value="BPD_transp_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="36"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="62"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="87"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="123"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="178"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="256"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEF96249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="288" mass="31554" checksum="6427EB86B071664D" modified="2011-07-27" version="1">MDITNYILDFIVHAGIFAILAVSLNLEFGYTGLSNFGKVAFFAIGAYTAALSNPFLPFPLNIILGVIIAGIAGFLISIPSLKLRDDYLAIVTIAFGEILRLIFNNEKWTGGASGWTNIQYFDLKTMLIIVLIAFIGVFIFSEKLVNSPFGRLMKAVREDEIATKALGKDTFLIKAKTLGVGSALAGIAGGLYAHYLGYISPDMFMPIITFLIWMMVIIGGKANNYGVLVGVLIVDLIERGTQILGNYDLPIEPANLRMILIGLLLILFVMYRPYGLIPEKPMKIGEIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-06-02" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCP1</accession>
    <name>F6BCP1_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">SEC-C motif domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_0702</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96252.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798855.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96252">
        <property type="protein sequence ID" value="AEF96252"/>
        <property type="gene ID" value="Metig_0702"/>
    </dbReference>
    <dbReference type="GeneID" id="10643542"/>
    <dbReference type="KEGG" id="mig:Metig_0702"/>
    <dbReference type="HOGENOM" id="CLU_1025313_0_0_2"/>
    <dbReference type="OMA" id="YKEENDW"/>
    <dbReference type="OrthoDB" id="137711at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004027">
        <property type="entry name" value="SEC_C_motif"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02810">
        <property type="entry name" value="SEC-C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="SSF48371"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF96252.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="271" mass="32161" checksum="AE5708D1CD3104BB" modified="2011-07-27" version="1">MKNPSPKTTEELLKELAKRKIVVNKELIKEIVKREDAPKEILRLINELNEISDMWFPIHAIYILGLIRTKEALNTLKYIIKNFDIDDFVGDLPDVFYNFGAEYFDDIKEIVLDESYDEYIRLNAFESLFMMEDVDRNKLLEFSKEVFNKLLKGDKVSDNVKCFMAGLMLTLEDKDKGFCSEVFNFIVDVIKEYNERNKHSFFKLHIDDVLYYKEENDWKKPKDLWEFYHPKNLLHLFEVNYGKLSKIDKYGPCICGSGKKFSDCCLKYLKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCR5</accession>
    <name>F6BCR5_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Methanogenesis marker protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0726</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96276.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0726"/>
    <dbReference type="EnsemblBacteria" id="AEF96276">
        <property type="protein sequence ID" value="AEF96276"/>
        <property type="gene ID" value="Metig_0726"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0726"/>
    <dbReference type="HOGENOM" id="CLU_056369_0_0_2"/>
    <dbReference type="OMA" id="CHLHPEI"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017667">
        <property type="entry name" value="Methan_mark_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003776">
        <property type="entry name" value="YcaO-like_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02624">
        <property type="entry name" value="YcaO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03266">
        <property type="entry name" value="methan_mark_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00702">
        <property type="entry name" value="TIGR00702"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51664">
        <property type="entry name" value="YCAO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="YcaO" evidence="1">
        <location>
            <begin position="66"/>
            <end position="390"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96276.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="390" mass="44343" checksum="6BA9E17EF26C6810" modified="2011-07-27" version="1">MDEITYTLASYRVCSPEETWKKIEPILNDIGVTRIARIDGLDRVGIPVYSAIRPTAKEGAISVYSGKGATDIQARVSAAMEAIERYSAEQDEKTNVKTTQNPKNPIDVNELILPPNIKPNVDLWVEGYDIINDEFVEVPANAVFHPYEGRRLFRSNTNGLASGNTRDEAIFHGMLEVIERDAWSIAELSRRTYRGVNIEDAKNPLIHELMEKFRKAKINVILKDLTSEVGIPTIAAISDDDVLRDPALLCMGVGCHIHPEIAVLRALTEVAQSRATQIHGAREDAVRGDIVRKVSYERMKRIHKRWFEYKEEISISDIPNNAKLNLKKDMKFVKKRLVESGFERVIVVDLNKVGVDVVRVIIPKMEVYCIDRDRISPWARERIRNMVDKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCU5</accession>
    <name>F6BCU5_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0760</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96306.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798909.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0760"/>
    <dbReference type="EnsemblBacteria" id="AEF96306">
        <property type="protein sequence ID" value="AEF96306"/>
        <property type="gene ID" value="Metig_0760"/>
    </dbReference>
    <dbReference type="GeneID" id="10643599"/>
    <dbReference type="KEGG" id="mig:Metig_0760"/>
    <dbReference type="HOGENOM" id="CLU_075000_1_0_2"/>
    <dbReference type="OMA" id="ICLMGET"/>
    <dbReference type="OrthoDB" id="92721at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10900">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004426">
        <property type="entry name" value="CHP00062"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019151">
        <property type="entry name" value="Proteasome_assmbl_chaperone_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038389">
        <property type="entry name" value="PSMG2_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09754">
        <property type="entry name" value="PAC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00162">
        <property type="entry name" value="TIGR00162"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="206"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96306.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="255" mass="28593" checksum="A9D4FE3373B02734" modified="2011-07-27" version="1">MVKIIERKIRDVEPLENAILVEGLPGIGHVGRIAAEHIVREFKGEKFLELYCYDFPPQVLVNEDGTVEFMNNEFYIIREPIPMIVVLGNTQALSPIGQYYLAEKLVEIGIKYGAKMTYTLGGFGVGKITDVPKVYAAATSKELAEKLKEYGVEFRKDGGGIVGAAGLMLTFSKLNGIEGVCLMGETPGYLVDPKSARAVLEKFSKIVGFEIDMKELEERAKEMEKFLEKIRKFEEEVMMQQQPKPPSDEDLRYIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCZ7</accession>
    <name>F6BCZ7_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">TM1410 hypothetical-related protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0813</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96358.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798960.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96358">
        <property type="protein sequence ID" value="AEF96358"/>
        <property type="gene ID" value="Metig_0813"/>
    </dbReference>
    <dbReference type="GeneID" id="10643654"/>
    <dbReference type="KEGG" id="mig:Metig_0813"/>
    <dbReference type="HOGENOM" id="CLU_067038_1_0_2"/>
    <dbReference type="OMA" id="WPDERWI"/>
    <dbReference type="OrthoDB" id="62350at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004352">
        <property type="entry name" value="GH114_TIM-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="Glycoside_hydrolase_SF"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03537">
        <property type="entry name" value="Glyco_hydro_114"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="SSF51445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glyco_hydro_114" evidence="1">
        <location>
            <begin position="40"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03537"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96358.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="277" mass="32682" checksum="C0375BFAF14A95AC" modified="2011-07-27" version="1">MRKLLLTFLFLILFCGCLNNTKIADDKPNINNKLNIDNFWVYYGSNNIEKLSRYDLVIIEPYNYKKEDIQKLKSLNPNIKVIAYLSIGEVSKDRPYFKECQKIIVGKNQNWNSYYINISSPIWQKIILNEVKKFKNMGFDGVFLDTVDSAIYTNQKKEVIELIKKIRQENSNILIIQNRGFEVVDKTAPYIDGVLFEDFTTYYDFKNKKAYYWKGSDLNWINAQSEKLKNLKEKYNLVILTLDYVNDIEMAKKCIEHAKKYNFIPMTTKDITLNSIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>F6BD16</accession>
    <name>F6BD16_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">AMP phosphorylase</fullName>
            <shortName evidence="3">AMPpase</shortName>
            <ecNumber evidence="3">2.4.2.57</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Nucleoside monophosphate phosphorylase</fullName>
            <shortName evidence="3">NMP phosphorylase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Metig_0832</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the conversion of AMP and phosphate to adenine and ribose 1,5-bisphosphate (R15P). Exhibits phosphorylase activity toward CMP and UMP in addition to AMP. Functions in an archaeal AMP degradation pathway, together with R15P isomerase and RubisCO.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>AMP + phosphate = adenine + alpha-D-ribose 1,5-bisphosphate</text>
            <dbReference type="Rhea" id="RHEA:36975"/>
            <dbReference type="ChEBI" id="CHEBI:16708"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:68688"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.4.2.57"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>CMP + phosphate = alpha-D-ribose 1,5-bisphosphate + cytosine</text>
            <dbReference type="Rhea" id="RHEA:36987"/>
            <dbReference type="ChEBI" id="CHEBI:16040"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="ChEBI" id="CHEBI:68688"/>
            <dbReference type="EC" id="2.4.2.57"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>phosphate + UMP = alpha-D-ribose 1,5-bisphosphate + uracil</text>
            <dbReference type="Rhea" id="RHEA:36991"/>
            <dbReference type="ChEBI" id="CHEBI:17568"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
            <dbReference type="ChEBI" id="CHEBI:68688"/>
            <dbReference type="EC" id="2.4.2.57"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the thymidine/pyrimidine-nucleoside phosphorylase family. Type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.57" evidence="3"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96377.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798979.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0832"/>
    <dbReference type="EnsemblBacteria" id="AEF96377">
        <property type="protein sequence ID" value="AEF96377"/>
        <property type="gene ID" value="Metig_0832"/>
    </dbReference>
    <dbReference type="GeneID" id="10643673"/>
    <dbReference type="KEGG" id="mig:Metig_0832"/>
    <dbReference type="HOGENOM" id="CLU_025040_6_0_2"/>
    <dbReference type="OMA" id="DVWRRMI"/>
    <dbReference type="OrthoDB" id="43931at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004645">
        <property type="term" value="F:1,4-alpha-oligoglucan phosphorylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016208">
        <property type="term" value="F:AMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016763">
        <property type="term" value="F:pentosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006196">
        <property type="term" value="P:AMP catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046125">
        <property type="term" value="P:pyrimidine deoxyribonucleoside metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006206">
        <property type="term" value="P:pyrimidine nucleobase metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1030.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1170.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02132">
        <property type="entry name" value="AMP_phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017713">
        <property type="entry name" value="AMP_phosphorylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000312">
        <property type="entry name" value="Glycosyl_Trfase_fam3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017459">
        <property type="entry name" value="Glycosyl_Trfase_fam3_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036320">
        <property type="entry name" value="Glycosyl_Trfase_fam3_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035902">
        <property type="entry name" value="Nuc_phospho_transferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036566">
        <property type="entry name" value="PYNP-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013102">
        <property type="entry name" value="PYNP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017872">
        <property type="entry name" value="Pyrmidine_PPase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013466">
        <property type="entry name" value="Thymidine/AMP_Pase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000053">
        <property type="entry name" value="Thymidine/pyrmidine_PPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10515">
        <property type="entry name" value="PTHR10515"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02885">
        <property type="entry name" value="Glycos_trans_3N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00591">
        <property type="entry name" value="Glycos_transf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07831">
        <property type="entry name" value="PYNP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000478">
        <property type="entry name" value="TP_PyNP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00941">
        <property type="entry name" value="PYNP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47648">
        <property type="entry name" value="SSF47648"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52418">
        <property type="entry name" value="SSF52418"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54680">
        <property type="entry name" value="SSF54680"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03327">
        <property type="entry name" value="AMP_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02645">
        <property type="entry name" value="ARCH_P_rylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00647">
        <property type="entry name" value="THYMID_PHOSPHORYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3 5" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PYNP_C" evidence="4">
        <location>
            <begin position="426"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="3">
        <location>
            <position position="258"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="170"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="196"/>
            <end position="201"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="205"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="266"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="290"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEF96377.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="505" mass="54490" checksum="B5F40A77C5046779" modified="2011-07-27" version="1">MLFLKAKLFDLDIGINAVIMNEEDLKGTPYYPQDRVLVETPAGSFVGVLHSTKTLVNKGEIGIFREITKEIPVKDGDEVIIRHAETPLSVAYIRKKMDGQTLKPEEIHTIIDEMVAGKLSNIENTAFVTALYINGMDMKEIEEMTKRMAETGDMIDWEESLVVDVHSIGGVPGNKTALLVVPIVAAAGLTIPKTSSRAITSAAGTADVVEVLTRVDLSLDEIKQVVTTTNGCMVWGGAIDLAPADDITINVERPLSIDPEPLLLSSVMSKKIAAGIKYLVIDIPTGEGAKVKTVDEAASLARKFIELGERVNVHVECAITYGGQPIGRAIGPALEAKEALMALENYKEAPSSLIEKALSLAGMLLEMGGVAPHGRGKSVAEEILASGKALEKMHEIITAQGGEPKSSDEIEVGEYKAEIIAPMDGYVTQISNKLITKVAKEAGAPRDKKAGVYLNVKIGNKVEKGDVLYTIYSSSEERLNAAIKLARRTYPVKVEGMLLKRISRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>F6BD17</accession>
    <name>F6BD17_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Molybdenum cofactor synthesis domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metig_0833</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; molybdopterin biosynthesis.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96378.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798980.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0833"/>
    <dbReference type="EnsemblBacteria" id="AEF96378">
        <property type="protein sequence ID" value="AEF96378"/>
        <property type="gene ID" value="Metig_0833"/>
    </dbReference>
    <dbReference type="GeneID" id="10643674"/>
    <dbReference type="KEGG" id="mig:Metig_0833"/>
    <dbReference type="HOGENOM" id="CLU_010186_7_2_2"/>
    <dbReference type="OMA" id="MTGAMVP"/>
    <dbReference type="OrthoDB" id="51611at2157"/>
    <dbReference type="UniPathway" id="UPA00344"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006777">
        <property type="term" value="P:Mo-molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032324">
        <property type="term" value="P:molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00887">
        <property type="entry name" value="MoeA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.340.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.980.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036425">
        <property type="entry name" value="MoaB/Mog-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001453">
        <property type="entry name" value="MoaB/Mog_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008284">
        <property type="entry name" value="MoCF_biosynth_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038987">
        <property type="entry name" value="MoeA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005111">
        <property type="entry name" value="MoeA_C_domain_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036688">
        <property type="entry name" value="MoeA_C_domain_IV_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005110">
        <property type="entry name" value="MoeA_linker/N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036135">
        <property type="entry name" value="MoeA_linker/N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10192">
        <property type="entry name" value="PTHR10192"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00994">
        <property type="entry name" value="MoCF_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03454">
        <property type="entry name" value="MoeA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03453">
        <property type="entry name" value="MoeA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00852">
        <property type="entry name" value="MoCF_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53218">
        <property type="entry name" value="SSF53218"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63867">
        <property type="entry name" value="SSF63867"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63882">
        <property type="entry name" value="SSF63882"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00177">
        <property type="entry name" value="molyb_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01079">
        <property type="entry name" value="MOCF_BIOSYNTHESIS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0501">Molybdenum cofactor biosynthesis</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MoCF_biosynth" evidence="3">
        <location>
            <begin position="180"/>
            <end position="323"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEF96378.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="395" mass="44243" checksum="2D39401E96C2C195" modified="2011-07-27" version="1">MGKMLKRLISYNKARDIVFNEWDKFLMDKIKIVPLLDALDKVSHEDIESPVDLPMFDRAAMDGYAVRAEDTFGASTTNPLILNLVDSEDIDEGECVKVFTGSKMPKNANAVVMKEYCNEEDDFVEIYKGVHPYENVSRTGEDVKRGDILLKKGEIISPYHIAILSSVGIKEIKVYDVNVGIIATGDELVDLEEVNNMEELKNKSKIINSNSLMLYALVKEAGLNPKIYGKIDDDKEKIKSAVKKAITENDIVITTGGTSVGDRDYIVEVINELGNIIIHGVQIRPGKPFGFGKINNKLIFMLSGYPVASAVQFELFIRSYFKKRKKIKLPLKRNIASELGRTDIVRVKIEDSEVEPLRITGSGVISSLTKADGYIIIPENVEGYEKGEYVEVYLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>F6BD43</accession>
    <name>F6BD43_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">30S ribosomal protein S4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">rps4</name>
        <name evidence="9" type="ordered locus">Metig_0860</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">One of the primary rRNA binding proteins, it binds directly to 16S rRNA where it nucleates assembly of the body of the 30S subunit.</text>
    </comment>
    <comment type="function">
        <text evidence="6">With S5 and S12 plays an important role in translational accuracy.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Part of the 30S ribosomal subunit. Contacts protein S5. The interaction surface between S4 and S5 is involved in control of translational fidelity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the universal ribosomal protein uS4 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96404.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799006.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0860"/>
    <dbReference type="EnsemblBacteria" id="AEF96404">
        <property type="protein sequence ID" value="AEF96404"/>
        <property type="gene ID" value="Metig_0860"/>
    </dbReference>
    <dbReference type="GeneID" id="10643703"/>
    <dbReference type="KEGG" id="mig:Metig_0860"/>
    <dbReference type="HOGENOM" id="CLU_089738_1_1_2"/>
    <dbReference type="OMA" id="ARQFITH"/>
    <dbReference type="OrthoDB" id="93256at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00165">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.290.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01306_A">
        <property type="entry name" value="Ribosomal_S4_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022801">
        <property type="entry name" value="Ribosomal_S4/S9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005710">
        <property type="entry name" value="Ribosomal_S4/S9_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001912">
        <property type="entry name" value="Ribosomal_S4/S9_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022802">
        <property type="entry name" value="Ribosomal_S4_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018079">
        <property type="entry name" value="Ribosomal_S4_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002942">
        <property type="entry name" value="S4_RNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036986">
        <property type="entry name" value="S4_RNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11831">
        <property type="entry name" value="PTHR11831"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01479">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01390">
        <property type="entry name" value="Ribosomal_S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00363">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01018">
        <property type="entry name" value="uS4_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00632">
        <property type="entry name" value="RIBOSOMAL_S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 6 9" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3 6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2 6" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="Ribosomal_S4" evidence="8">
        <location>
            <begin position="5"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="domain" description="S4" evidence="7">
        <location>
            <begin position="104"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM01390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AEF96404.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="192" mass="22418" checksum="2D820B16D476155C" modified="2011-07-27" version="1">MGDPKKPRKKYDTPNHPWIKERIEREHELCRKYGLVNKREVWKAETKLRNYRRQARRLISDTTKQGAREAQQLFNVLKRYGILKKENPTLDDVLALTIEDILERRLQTIVFRKGLARTIKQARQLIVHGHIAVNGRKVTAPSYMVTVDEEDTIDYAPNSPFASKDHPERVKIMSVEAKEGEESQEQVSQTTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-06-02" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>F6BD54</accession>
    <name>F6BD54_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">SNARE associated Golgi protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0871</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96415.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799017.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96415">
        <property type="protein sequence ID" value="AEF96415"/>
        <property type="gene ID" value="Metig_0871"/>
    </dbReference>
    <dbReference type="GeneID" id="10643715"/>
    <dbReference type="KEGG" id="mig:Metig_0871"/>
    <dbReference type="HOGENOM" id="CLU_098634_2_0_2"/>
    <dbReference type="OMA" id="IFQPIPT"/>
    <dbReference type="OrthoDB" id="84854at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032816">
        <property type="entry name" value="SNARE_assoc"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09335">
        <property type="entry name" value="SNARE_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="53"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="100"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="132"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96415.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="161" mass="18279" checksum="488799BD114B80A0" modified="2011-07-27" version="1">MDFEIFKDVGLNLVKEYGYLGIFLIGLSEPIFQPIPTEIFIISGVLLGLDWKLVLIVSTLGCNLGAIITYCLATKYGEKLALRLFDEEKLKKGEKFLKKWGVMGVIVVSFTPIPFEIICWVCGMFNMPFEKYMVAVFLSRMIKHGLAVLPFVLPIKNYLPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>F6BD71</accession>
    <name>F6BD71_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Ferrous iron transport protein B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">Metig_0889</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96432.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799034.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0889"/>
    <dbReference type="EnsemblBacteria" id="AEF96432">
        <property type="protein sequence ID" value="AEF96432"/>
        <property type="gene ID" value="Metig_0889"/>
    </dbReference>
    <dbReference type="GeneID" id="10643733"/>
    <dbReference type="KEGG" id="mig:Metig_0889"/>
    <dbReference type="HOGENOM" id="CLU_013350_3_0_2"/>
    <dbReference type="OMA" id="MEMPDYE"/>
    <dbReference type="OrthoDB" id="27688at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015093">
        <property type="term" value="F:ferrous iron transmembrane transporter activity"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="F:GTP binding"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Fe2_transport_prot-B"/>
    </dbReference>
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        <property type="entry name" value="Fe2_transport_prot_B_C"/>
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    <dbReference type="InterPro" id="IPR041069">
        <property type="entry name" value="FeoB_Cyto"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030389">
        <property type="entry name" value="G_FEOB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011642">
        <property type="entry name" value="Gate_dom"/>
    </dbReference>
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        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07664">
        <property type="entry name" value="FeoB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17910">
        <property type="entry name" value="FeoB_Cyto"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02421">
        <property type="entry name" value="FeoB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07670">
        <property type="entry name" value="Gate"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00437">
        <property type="entry name" value="feoB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51711">
        <property type="entry name" value="G_FEOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7" id="KW-0408">Iron</keyword>
    <keyword evidence="10 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="262"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="318"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="367"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="401"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="433"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="491"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="522"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="549"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="588"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="619"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="domain" description="FeoB-type G" evidence="12">
        <location>
            <begin position="1"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51711"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AEF96432.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="642" mass="72227" checksum="D20BB3D6A5DC5295" modified="2011-07-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>F6BD74</accession>
    <name>F6BD74_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Metig_0892</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96435.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799037.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0892"/>
    <dbReference type="EnsemblBacteria" id="AEF96435">
        <property type="protein sequence ID" value="AEF96435"/>
        <property type="gene ID" value="Metig_0892"/>
    </dbReference>
    <dbReference type="GeneID" id="10643736"/>
    <dbReference type="KEGG" id="mig:Metig_0892"/>
    <dbReference type="HOGENOM" id="CLU_121274_0_0_2"/>
    <dbReference type="OMA" id="LGWYILC"/>
    <dbReference type="OrthoDB" id="94214at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033180">
        <property type="term" value="C:proton-transporting V-type ATPase, V1 domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015078">
        <property type="term" value="F:proton transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002809">
        <property type="entry name" value="EMC3/TMCO1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038978">
        <property type="entry name" value="MJ0935"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036132">
        <property type="entry name" value="Vac_ATP_synth_c_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42198">
        <property type="entry name" value="PTHR42198"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01956">
        <property type="entry name" value="EMC3_TMCO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01415">
        <property type="entry name" value="DUF106"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118203">
        <property type="entry name" value="SSF118203"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="27"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="103"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="141"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="174"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEF96435.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="198" mass="23346" checksum="84D76160EAFB9B3C" modified="2011-07-27" version="1">MFDFIFDVYYRTLDAIFMPLIEHLHPAIAILIIAFLVSFIINLATKLLVDQDKMAKLKKEIQEFQVKYKRAMKDPEMLQKLQEEQQKMMEMQMEMMKMSFKPMIYTWIPIIAIFAYLRHVYGYGGVFHTLYPDWNGVIVHLPYILSKILFVSFWHWLGSIFYKGGFGIVSDATLGWLGWYILCSMATSMVLRKVMGIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>F6BD95</accession>
    <name>F6BD95_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">30S ribosomal protein S3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">rps3</name>
        <name evidence="9" type="ordered locus">Metig_0913</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Binds the lower part of the 30S subunit head.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Part of the 30S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the universal ribosomal protein uS3 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96456.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799058.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0913"/>
    <dbReference type="EnsemblBacteria" id="AEF96456">
        <property type="protein sequence ID" value="AEF96456"/>
        <property type="gene ID" value="Metig_0913"/>
    </dbReference>
    <dbReference type="GeneID" id="10643757"/>
    <dbReference type="KEGG" id="mig:Metig_0913"/>
    <dbReference type="HOGENOM" id="CLU_058591_1_1_2"/>
    <dbReference type="OMA" id="YIKKCGE"/>
    <dbReference type="OrthoDB" id="60366at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1140.32">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01309_A">
        <property type="entry name" value="Ribosomal_S3_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015946">
        <property type="entry name" value="KH_dom-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004044">
        <property type="entry name" value="KH_dom_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009019">
        <property type="entry name" value="KH_sf_prok-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027488">
        <property type="entry name" value="Ribosomal_S3_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001351">
        <property type="entry name" value="Ribosomal_S3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036419">
        <property type="entry name" value="Ribosomal_S3_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005703">
        <property type="entry name" value="Ribosomal_S3_euk/arc"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07650">
        <property type="entry name" value="KH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00189">
        <property type="entry name" value="Ribosomal_S3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54814">
        <property type="entry name" value="SSF54814"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54821">
        <property type="entry name" value="SSF54821"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01008">
        <property type="entry name" value="uS3_euk_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50823">
        <property type="entry name" value="KH_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 6 9" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3 6 7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2 6" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="KH type-2" evidence="8">
        <location>
            <begin position="16"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AEF96456.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="209" mass="23460" checksum="3B941EAF146B906F" modified="2011-07-27" version="1">MIERVFVEESIKEMLIDEYFKKTLAKAGYSHMEIRKTPIGTKITIYAEKPGFVIGRKGKRVKELTETLEKTYKVNKPQIEVKPIEDPNLDPEVVAQKIATSLERGMHFRRVAHAAVRRVMNAGAKGVVVIISGKLTGERARTEKFMAGYMKHCGDPSETLVRKAFKLAKLKLGVIGVTVKIMPPDIVLPDEVIIKDDGEIKEVSEEVVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>F6BD99</accession>
    <name>F6BD99_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Aspartate/glutamate/uridylate kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0917</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96460.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799062.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0917"/>
    <dbReference type="EnsemblBacteria" id="AEF96460">
        <property type="protein sequence ID" value="AEF96460"/>
        <property type="gene ID" value="Metig_0917"/>
    </dbReference>
    <dbReference type="GeneID" id="10643761"/>
    <dbReference type="KEGG" id="mig:Metig_0917"/>
    <dbReference type="HOGENOM" id="CLU_089197_0_0_2"/>
    <dbReference type="OMA" id="LPHSWDV"/>
    <dbReference type="OrthoDB" id="85553at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04240">
        <property type="entry name" value="AAK_UC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1160.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036393">
        <property type="entry name" value="AceGlu_kinase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001048">
        <property type="entry name" value="Asp/Glu/Uridylate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011375">
        <property type="entry name" value="MfnE"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00696">
        <property type="entry name" value="AA_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004857">
        <property type="entry name" value="Kin_aa_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53633">
        <property type="entry name" value="SSF53633"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="AA_kinase" evidence="1">
        <location>
            <begin position="3"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96460.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="209" mass="23522" checksum="454A4DB43322F713" modified="2011-07-27" version="1">MHLIKIGGSLTYHAERLLNELKKIEKPIIIIPGGGEFANVVRKLYEKTNLNDSGAHKLATLCVDLMGLYFSEISGIETTDNLFDAKRILEKNGKIIFLPSKLILSADELPHSWDVTSDTIALYVAKFLNMEEIIVATDVDGVYDKYPGGKLLNTISAKDIRGFTSVDKHLPQLLIKYKMNCYVVNGKYPERIINLLNGKMDICTKITWF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDB0</accession>
    <name>F6BDB0_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="3">NADH ubiquinone oxidoreductase 20 kDa subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_0928</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96471.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799073.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0928"/>
    <dbReference type="EnsemblBacteria" id="AEF96471">
        <property type="protein sequence ID" value="AEF96471"/>
        <property type="gene ID" value="Metig_0928"/>
    </dbReference>
    <dbReference type="GeneID" id="10643774"/>
    <dbReference type="KEGG" id="mig:Metig_0928"/>
    <dbReference type="HOGENOM" id="CLU_053270_0_0_2"/>
    <dbReference type="OMA" id="GSWPEDI"/>
    <dbReference type="OrthoDB" id="44934at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.700">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006137">
        <property type="entry name" value="NADH_UbQ_OxRdtase-like_20kDa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037024">
        <property type="entry name" value="NiFe_Hase_small_N_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01058">
        <property type="entry name" value="Oxidored_q6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0830">Ubiquinone</keyword>
    <feature type="domain" description="Oxidored_q6" evidence="2">
        <location>
            <begin position="15"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF96471.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="287" mass="30963" checksum="A6489518DE3844E1" modified="2011-07-27" version="1">MADKVKVAMIQLCGCSGCHISLLDLHDKLLEVLPNLEIVYAPIVADPKEIPDGIDVALLEGGVRNEHDEHLVHEFREKAKIVIAWGTCAAYGGIPGLGNLYTKEELINRVYSTESTDNPGTIPSEEIPPLEDYVKPIPDVIKVDYIIPGCPPTPDMIASAIVALLNGEEPKFSTKIVCDECPRKKENVFPEKFKRTFEGKPDPEKCLFEQGYTCLGMATRAGCGAKCPNAGVPCRGCYGKTDAVLDQGAAAANTFANAGEAALEIPDKVALLNRFSLPAALISKKIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDB6</accession>
    <name>F6BDB6_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">tRNA pseudouridine synthase A</fullName>
            <ecNumber evidence="4">5.4.99.12</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">tRNA pseudouridine(38-40) synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">tRNA pseudouridylate synthase I</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">tRNA-uridine isomerase I</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">truA</name>
        <name evidence="9" type="ordered locus">Metig_0934</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4 7">
            <text>uridine(38/39/40) in tRNA = pseudouridine(38/39/40) in tRNA</text>
            <dbReference type="Rhea" id="RHEA:22376"/>
            <dbReference type="Rhea" id="RHEA-COMP:10085"/>
            <dbReference type="Rhea" id="RHEA-COMP:10087"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="EC" id="5.4.99.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 4 7">Belongs to the tRNA pseudouridine synthase TruA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="5.4.99.12" evidence="4"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96477.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799079.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0934"/>
    <dbReference type="EnsemblBacteria" id="AEF96477">
        <property type="protein sequence ID" value="AEF96477"/>
        <property type="gene ID" value="Metig_0934"/>
    </dbReference>
    <dbReference type="GeneID" id="10643780"/>
    <dbReference type="KEGG" id="mig:Metig_0934"/>
    <dbReference type="HOGENOM" id="CLU_014673_4_2_2"/>
    <dbReference type="OMA" id="MYIFKIA"/>
    <dbReference type="OrthoDB" id="62389at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106029">
        <property type="term" value="F:tRNA pseudouridine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031119">
        <property type="term" value="P:tRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.580">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.660">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00171">
        <property type="entry name" value="TruA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001406">
        <property type="entry name" value="PsdUridine_synth_TruA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020097">
        <property type="entry name" value="PsdUridine_synth_TruA_a/b_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020095">
        <property type="entry name" value="PsdUridine_synth_TruA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020094">
        <property type="entry name" value="TruA/RsuA/RluB/E/F_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11142">
        <property type="entry name" value="PTHR11142"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01416">
        <property type="entry name" value="PseudoU_synth_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001430">
        <property type="entry name" value="tRNA_psdUrid_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="SSF55120"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00071">
        <property type="entry name" value="hisT_truA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4 7" id="KW-0413">Isomerase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4 7" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="PseudoU_synth_1" evidence="8">
        <location>
            <begin position="118"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="4 5">
        <location>
            <position position="50"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4 6">
        <location>
            <position position="101"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR001430-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR001430-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AEF96477.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="265" mass="30897" checksum="DD265B05D68F53AD" modified="2011-07-27" version="1">MYILKVAYDGRYSFQSQPHRNTVVDVLMDALKECGYLKEEKVIFSGGRTDKGVSALGNFVVVELNKEPILSFIYSKLRGKGIWVLGYREIDEIPKVEFRHYRYILPDEGYNIELMREASKKLIGTHSFHNLSKKDRSKERSPIRTIYDIKIDANEYFITIDVIGESFLWNMVRKIVTALSYVGSEKKPVDWIDKLLDTHYKEGVPPAPAEGLILIEAKVNVEYIYDNYVLRRFREEWEEEYGKSIMKLGICKSMLNFLNKPKNSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDF1</accession>
    <name>F6BDF1_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">APHP domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0969</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96512.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799114.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0969"/>
    <dbReference type="EnsemblBacteria" id="AEF96512">
        <property type="protein sequence ID" value="AEF96512"/>
        <property type="gene ID" value="Metig_0969"/>
    </dbReference>
    <dbReference type="GeneID" id="10643815"/>
    <dbReference type="KEGG" id="mig:Metig_0969"/>
    <dbReference type="HOGENOM" id="CLU_360805_0_0_2"/>
    <dbReference type="OrthoDB" id="2301at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011635">
        <property type="entry name" value="CARDB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007742">
        <property type="entry name" value="NosD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006626">
        <property type="entry name" value="PbH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012334">
        <property type="entry name" value="Pectin_lyas_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026495">
        <property type="entry name" value="PIP_CTERM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022409">
        <property type="entry name" value="PKD/Chitinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000601">
        <property type="entry name" value="PKD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035986">
        <property type="entry name" value="PKD_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07705">
        <property type="entry name" value="CARDB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05048">
        <property type="entry name" value="NosD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00710">
        <property type="entry name" value="PbH1"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00089">
        <property type="entry name" value="PKD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49299">
        <property type="entry name" value="SSF49299"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="SSF51126"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR04173">
        <property type="entry name" value="PIP_CTERM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50093">
        <property type="entry name" value="PKD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PKD" evidence="1">
        <location>
            <begin position="607"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="domain" description="PKD" evidence="1">
        <location>
            <begin position="658"/>
            <end position="735"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96512.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="775" mass="85143" checksum="4DDBBBE20C5000A6" modified="2011-07-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDG0</accession>
    <name>F6BDG0_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Metal dependent phosphohydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_0978</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96521.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799123.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0978"/>
    <dbReference type="EnsemblBacteria" id="AEF96521">
        <property type="protein sequence ID" value="AEF96521"/>
        <property type="gene ID" value="Metig_0978"/>
    </dbReference>
    <dbReference type="GeneID" id="10643824"/>
    <dbReference type="KEGG" id="mig:Metig_0978"/>
    <dbReference type="HOGENOM" id="CLU_1109506_0_0_2"/>
    <dbReference type="OMA" id="IRICQAR"/>
    <dbReference type="OrthoDB" id="56504at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006674">
        <property type="entry name" value="HD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006675">
        <property type="entry name" value="HDIG_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01966">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00471">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00277">
        <property type="entry name" value="HDIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51831">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HD" evidence="2">
        <location>
            <begin position="58"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="229"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF96521.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="250" mass="28967" checksum="B17A9F0C31C5C495" modified="2011-07-27" version="1">MEKLLELANQIKDEKLREKVIEFLKNPIPTHKEIEDTKIPPESSPASIKWHHKYEGGLIEHTIAVTKLALKMADVLEEVYGIKVDRDLIIAGGLLHDIMKPFNYIKEDDKFDHIERFHLDHLTLAVAELYKRDFPLEVIKIVASHHGDASPSRPNSIEAYLIYFADTFDAQLNDVAIRVCQARSKDLGIDEGEIYKRITPLKVYEIRSKEGKKKLIEYLKELLGIKEEQTEEEISEENHEENNKTENQQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDG1</accession>
    <name>F6BDG1_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">tRNA (guanine(26)-N(2))-dimethyltransferase</fullName>
            <ecNumber evidence="7">2.1.1.216</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">tRNA 2,2-dimethylguanosine-26 methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">tRNA(guanine-26,N(2)-N(2)) methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">tRNA(m(2,2)G26)dimethyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">trm1</name>
        <name evidence="9" type="ordered locus">Metig_0979</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Dimethylates a single guanine residue at position 26 of a number of tRNAs using S-adenosyl-L-methionine as donor of the methyl groups.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>guanosine(26) in tRNA + 2 S-adenosyl-L-methionine = 2 H(+) + N(2)-dimethylguanosine(26) in tRNA + 2 S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:43140"/>
            <dbReference type="Rhea" id="RHEA-COMP:10359"/>
            <dbReference type="Rhea" id="RHEA-COMP:10360"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74269"/>
            <dbReference type="ChEBI" id="CHEBI:74513"/>
            <dbReference type="EC" id="2.1.1.216"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="7 8">Belongs to the class I-like SAM-binding methyltransferase superfamily. Trm1 family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.216" evidence="7"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96522.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799124.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0979"/>
    <dbReference type="EnsemblBacteria" id="AEF96522">
        <property type="protein sequence ID" value="AEF96522"/>
        <property type="gene ID" value="Metig_0979"/>
    </dbReference>
    <dbReference type="GeneID" id="10643825"/>
    <dbReference type="KEGG" id="mig:Metig_0979"/>
    <dbReference type="HOGENOM" id="CLU_010862_5_1_2"/>
    <dbReference type="OMA" id="VFYNPVM"/>
    <dbReference type="OrthoDB" id="70865at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004809">
        <property type="term" value="F:tRNA (guanine-N2-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.56.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00290">
        <property type="entry name" value="tRNA_dimethyltr_TRM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002905">
        <property type="entry name" value="Trm1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022923">
        <property type="entry name" value="TRM1_arc_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042296">
        <property type="entry name" value="tRNA_met_Trm1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10631">
        <property type="entry name" value="PTHR10631"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02005">
        <property type="entry name" value="TRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00308">
        <property type="entry name" value="TRM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51626">
        <property type="entry name" value="SAM_MT_TRM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7 8" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 8" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4 7 8" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3 7 8" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 7 8" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="1 8" id="KW-0820">tRNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AEF96522.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="372" mass="41829" checksum="714E225F52A204C1" modified="2011-07-27" version="1">MIVVEGKTKITVPDTLTITKKDEVFYNPKMEANRDISVCVIQTFLKNYKREEFLICDPLGGSGARGLRYANELEFKNGIVKVVINDINPKAVKLIKENIKLNNLDNVEVYEGDANVILSKNFRMFNVVDLDPFGSPSPYLDSGIRATITKNGLLCLTATDTAVLCGRFKKPCVRKYNAFPINSKDCHEMAIRILIGYAIRVAAKYDIGLKPIFSHATDHYVRTYLLTQRGAKKADEALEKLGYVKDIGGDKVIKSLYEGYEKGFGGPLYLGEIYDKEIVNEVYKIAVERNYSEKAKNILKVLCDECDINQLGCYDIHEICSNIKKSAPPMTTIIEKLKERGFKVSRVHYNPKGIKSDAKLSDIIEVILEATD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDI3</accession>
    <name>F6BDI3_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">(5-formylfuran-3-yl)methyl phosphate synthase</fullName>
            <ecNumber evidence="5">4.2.3.153</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">4-(hydroxymethyl)-2-furancarboxaldehyde-phosphate synthase</fullName>
            <shortName evidence="5">4-HFC-P synthase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">mfnB</name>
        <name evidence="7" type="ordered locus">Metig_1002</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 5">Catalyzes the formation of 4-(hydroxymethyl)-2-furancarboxaldehyde phosphate (4-HFC-P) from two molecules of glyceraldehyde-3-P (GA-3-P).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 5">
            <text>2 D-glyceraldehyde 3-phosphate = 4-(hydroxymethyl)-2-furancarboxaldehyde phosphate + 2 H2O + phosphate</text>
            <dbReference type="Rhea" id="RHEA:43536"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="ChEBI" id="CHEBI:83407"/>
            <dbReference type="EC" id="4.2.3.153"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="5">Cofactor biosynthesis; methanofuran biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the MfnB family.</text>
    </comment>
    <dbReference type="EC" id="4.2.3.153" evidence="5"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96544.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799146.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1002"/>
    <dbReference type="EnsemblBacteria" id="AEF96544">
        <property type="protein sequence ID" value="AEF96544"/>
        <property type="gene ID" value="Metig_1002"/>
    </dbReference>
    <dbReference type="GeneID" id="10643850"/>
    <dbReference type="KEGG" id="mig:Metig_1002"/>
    <dbReference type="HOGENOM" id="CLU_068659_0_0_2"/>
    <dbReference type="OMA" id="NFPWVIR"/>
    <dbReference type="OrthoDB" id="71837at2157"/>
    <dbReference type="UniPathway" id="UPA00080"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016830">
        <property type="term" value="F:carbon-carbon lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001120">
        <property type="term" value="P:methanofuran biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00681">
        <property type="entry name" value="MfnB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007565">
        <property type="entry name" value="4HFCP_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035081">
        <property type="entry name" value="4HFCP_synth_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036206">
        <property type="entry name" value="ThiamineP_synth_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04476">
        <property type="entry name" value="4HFCP_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015957">
        <property type="entry name" value="UCP015957"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51391">
        <property type="entry name" value="SSF51391"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0456">Lyase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0704">Schiff base</keyword>
    <feature type="active site" description="Schiff-base intermediate with substrate" evidence="5 6">
        <location>
            <position position="27"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="5 6">
        <location>
            <position position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR015957-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEF96544.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="236" mass="25417" checksum="92CF463F0F826B45" modified="2011-07-27" version="1">MLLLVSPKDVNEAIEAIEGGADIIDVKNPVEGSLGANFPWVIKEIREVTPKNLLVSATIGDVPYKPGTAALAAMGAAISGADYIKVGLYGTKSYNQAVDLMKKVVKAVKDIDENKIVVAAGYADAYRVGAVEPLVIPKVARDSGCDVAMLDTAIKDGKTLFDHLDKETLREFVEETHRYGLKCALAGSIKKEHIPILKEIGCDIVGVRGAACTKGDRNEGRIERELVKELVELCRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDI9</accession>
    <name>F6BDI9_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">2-phospho-L-lactate guanylyltransferase</fullName>
            <shortName evidence="1">LP guanylyltransferase</shortName>
            <ecNumber evidence="1">2.7.7.68</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">cofC</name>
        <name evidence="2" type="ordered locus">Metig_1008</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Guanylyltransferase that catalyzes the activation of (2S)-2-phospholactate (2-PL) as (2S)-lactyl-2-diphospho-5'-guanosine, via the condensation of 2-PL with GTP. It is involved in the biosynthesis of coenzyme F420, a hydride carrier cofactor.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(2S)-2-phospholactate + GTP + H(+) = (2S)-lactyl-2-diphospho-5'-guanosine + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:63424"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:59435"/>
            <dbReference type="ChEBI" id="CHEBI:59906"/>
            <dbReference type="EC" id="2.7.7.68"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; coenzyme F420 biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the CofC family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.68" evidence="1"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96550.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799152.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1008"/>
    <dbReference type="EnsemblBacteria" id="AEF96550">
        <property type="protein sequence ID" value="AEF96550"/>
        <property type="gene ID" value="Metig_1008"/>
    </dbReference>
    <dbReference type="GeneID" id="10643862"/>
    <dbReference type="KEGG" id="mig:Metig_1008"/>
    <dbReference type="HOGENOM" id="CLU_076569_2_0_2"/>
    <dbReference type="OMA" id="FRVDYHG"/>
    <dbReference type="OrthoDB" id="67356at2157"/>
    <dbReference type="UniPathway" id="UPA00071"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043814">
        <property type="term" value="F:phospholactate guanylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052645">
        <property type="term" value="P:F420-0 metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02114">
        <property type="entry name" value="CofC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002835">
        <property type="entry name" value="CofC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40392">
        <property type="entry name" value="PTHR40392"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01983">
        <property type="entry name" value="CofC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03552">
        <property type="entry name" value="F420_cofC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="1 2" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96550.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="216" mass="24182" checksum="145ACD3AF17D0F96" modified="2011-07-27" version="1">MIAVIPVSPLNSAKSRLSEFLTGEERKLLLMNMIMDVYNALEDLEVCLISKDDEILNFSKELGLIPLKERGKGLNNAIKQAINAVEDEEVIVVPADIPLIKKYHIKEVISKSKKNSIVISPSRGGGTNLLLLNPKDIIEPKYEGFSFLKHIDEAKKRNVEVIVYDSFLISIDVNTPEDLGEVFIHGEGTHTYKYLKSLGIEVLPKHSSAGRFIIKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDK8</accession>
    <name>F6BDK8_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">30S ribosomal protein S24e</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">rps24e</name>
        <name evidence="5" type="ordered locus">Metig_1027</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein eS24 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96569.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799171.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1027"/>
    <dbReference type="EnsemblBacteria" id="AEF96569">
        <property type="protein sequence ID" value="AEF96569"/>
        <property type="gene ID" value="Metig_1027"/>
    </dbReference>
    <dbReference type="GeneID" id="10643881"/>
    <dbReference type="KEGG" id="mig:Metig_1027"/>
    <dbReference type="HOGENOM" id="CLU_107248_3_1_2"/>
    <dbReference type="OMA" id="QYIRSYF"/>
    <dbReference type="OrthoDB" id="124805at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00545">
        <property type="entry name" value="Ribosomal_S24e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012678">
        <property type="entry name" value="Ribosomal_L23/L15e_core_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001976">
        <property type="entry name" value="Ribosomal_S24e"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01282">
        <property type="entry name" value="Ribosomal_S24e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54189">
        <property type="entry name" value="SSF54189"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 5" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEF96569.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="100" mass="11618" checksum="A28877C1C6E324F3" modified="2011-07-27" version="1">MEIKIISDRQNPLLSRREIRFIVTFEGATPTIKDVKLKLAAMLNVDKNMLIIDRIDQEFGKQEARGYAKLYFDEKIMNMVEKKSVLEKNKIEEEVAAEGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDL6</accession>
    <name>F6BDL6_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Glutamine amidotransferase class-II</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_1035</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96577.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799179.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1035"/>
    <dbReference type="MEROPS" id="C44.A09"/>
    <dbReference type="EnsemblBacteria" id="AEF96577">
        <property type="protein sequence ID" value="AEF96577"/>
        <property type="gene ID" value="Metig_1035"/>
    </dbReference>
    <dbReference type="GeneID" id="10643889"/>
    <dbReference type="KEGG" id="mig:Metig_1035"/>
    <dbReference type="HOGENOM" id="CLU_061941_0_0_2"/>
    <dbReference type="OMA" id="FKGVGFP"/>
    <dbReference type="OrthoDB" id="55229at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006541">
        <property type="term" value="P:glutamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017932">
        <property type="entry name" value="GATase_2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012375">
        <property type="entry name" value="Glu_synth_lsu_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018774">
        <property type="entry name" value="GOGAT_lg_dom1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="SSF56235"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51278">
        <property type="entry name" value="GATASE_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glutamine amidotransferase type-2" evidence="2">
        <location>
            <begin position="2"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="active site" description="For GATase activity" evidence="1">
        <location>
            <position position="2"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR018774-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF96577.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="361" mass="41564" checksum="1F5EEEB0D165F926" modified="2011-07-27" version="1">MCGIIGFISRDQKLIGGEKIATALNCLRERGNGKGAGYVGYGIYPKYADKYAIHVFLDSSKDYDKIRENVRYTLEKYGYVVKDEEIPVEEGVLEKEYVSWRFFYEFDEKYKPFEEDKMVELVMEINDKIDGAFVFSSGKNLGVFKAAAWPHEVAEYFKIDKYKGWMWISHARYPTNTRGWWGGAHPFNLLNWSVVHNGEITSYGTNKRYVESFGYKCRLFTDTEVVAYLLDLLVRKHKLPIEYAATALAPRFWDEIDRMPEEERKIHEAIRMTYGSAMLNGPFAIIVGTHEGMLFMNGDIEKGNAMFGLTDRIKLRPLVAAEKDDLFFVSSEEAAIRAICPDLDRVWMPDAGKMVIARVNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDM0</accession>
    <name>F6BDM0_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Nitrite and sulphite reductase 4Fe-4S region</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metig_1039</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96581.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799183.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1039"/>
    <dbReference type="EnsemblBacteria" id="AEF96581">
        <property type="protein sequence ID" value="AEF96581"/>
        <property type="gene ID" value="Metig_1039"/>
    </dbReference>
    <dbReference type="GeneID" id="10643893"/>
    <dbReference type="KEGG" id="mig:Metig_1039"/>
    <dbReference type="HOGENOM" id="CLU_072599_0_1_2"/>
    <dbReference type="OMA" id="KVRMHDF"/>
    <dbReference type="OrthoDB" id="58856at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.413.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005117">
        <property type="entry name" value="NiRdtase/SiRdtase_haem-b_fer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036136">
        <property type="entry name" value="Nit/Sulf_reduc_fer-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006067">
        <property type="entry name" value="NO2/SO3_Rdtase_4Fe4S_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045169">
        <property type="entry name" value="NO2/SO3_Rdtase_4Fe4S_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045854">
        <property type="entry name" value="NO2/SO3_Rdtase_4Fe4S_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006066">
        <property type="entry name" value="NO2/SO3_Rdtase_FeS/sirohaem_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11493">
        <property type="entry name" value="PTHR11493"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01077">
        <property type="entry name" value="NIR_SIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03460">
        <property type="entry name" value="NIR_SIR_ferr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55124">
        <property type="entry name" value="SSF55124"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56014">
        <property type="entry name" value="SSF56014"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00365">
        <property type="entry name" value="NIR_SIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0349">Heme</keyword>
    <keyword evidence="1" id="KW-0408">Iron</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="3">
        <location>
            <begin position="150"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEF96581.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="273" mass="30875" checksum="79A6C4DE022EAFF3" modified="2011-07-27" version="1">MFCNQNYKFGVIPKKDGYMIRLALKPGYITSEQLDAISYIAKNFGENKVHITTRQGIELKIKHEHLEEAEKILNEVGIKLGSTGRRIRQIVACIGTECPNSIGDSIKLARMIHDEFEGIWVPKKLKINVSGCPNNCTHHQFCDIGICFRYILKIDKKECTDCGKCRGFCEIDAIDWKNKVIKENCIGMGECLKLCNAIKVEDRVISIFIGGKGGKFPKKGKWLVDVKTEDEVIEVIDGIIELYTKFGKGRICDFVEHVGIENVKKSLKEKGFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDN0</accession>
    <name>F6BDN0_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">TatD-related deoxyribonuclease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_1050</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96591.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1050"/>
    <dbReference type="EnsemblBacteria" id="AEF96591">
        <property type="protein sequence ID" value="AEF96591"/>
        <property type="gene ID" value="Metig_1050"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_1050"/>
    <dbReference type="HOGENOM" id="CLU_985571_0_0_2"/>
    <dbReference type="OMA" id="CAVQLHT"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001130">
        <property type="entry name" value="TatD-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011589">
        <property type="entry name" value="UCP004961"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42206">
        <property type="entry name" value="PTHR42206"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01026">
        <property type="entry name" value="TatD_DNase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004961">
        <property type="entry name" value="UCP004961_TatD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="SSF51556"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF96591.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="295" mass="33272" checksum="57C1D3F595B7718A" modified="2011-07-27" version="1">MISKIVYAKKFFGDTMDILKNLPITDNHIHVDNENGYGAEKVAKIFHNAGGKVMIVLNKPTFDGDLTKSMDILVKDIERINKNTNVKAFGLVGVHPAELTVMLKRGISLEEAKEKMINALNYAKKLVEENDFIVGIGEVGRPHYEVSEDVWRVSNEILRYAMELAEDLNCAIQIHAESATEEQFKELSEMAKDVGLNPEKVIKHHCGDMVLEGEKYGIFPSIIASKPVESAVKKSLRFVMETDYIDDLKRPGVVLGIKTVPRRTRKLIEMGLLDEEGCFKIHKENIENLYGIEIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDN4</accession>
    <name>F6BDN4_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Lon proteolytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Metig_1055</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase S16 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96595.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799196.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1055"/>
    <dbReference type="MEROPS" id="S16.A12"/>
    <dbReference type="EnsemblBacteria" id="AEF96595">
        <property type="protein sequence ID" value="AEF96595"/>
        <property type="gene ID" value="Metig_1055"/>
    </dbReference>
    <dbReference type="GeneID" id="10643909"/>
    <dbReference type="KEGG" id="mig:Metig_1055"/>
    <dbReference type="HOGENOM" id="CLU_027628_0_0_2"/>
    <dbReference type="OMA" id="VMMTGMI"/>
    <dbReference type="OrthoDB" id="80994at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004176">
        <property type="term" value="F:ATP-dependent peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030163">
        <property type="term" value="P:protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008269">
        <property type="entry name" value="Lon_proteolytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027065">
        <property type="entry name" value="Lon_Prtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10046">
        <property type="entry name" value="PTHR10046"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05362">
        <property type="entry name" value="Lon_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51786">
        <property type="entry name" value="LON_PROTEOLYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0720">Serine protease</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="587"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="domain" description="Lon proteolytic" evidence="4">
        <location>
            <begin position="21"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="active site" evidence="2">
        <location>
            <position position="108"/>
        </location>
    </feature>
    <feature type="active site" evidence="2">
        <location>
            <position position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEF96595.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="611" mass="68639" checksum="3155AE3A428D1C8B" modified="2011-07-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDQ4</accession>
    <name>F6BDQ4_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Metig_1076</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96615.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799216.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1076"/>
    <dbReference type="EnsemblBacteria" id="AEF96615">
        <property type="protein sequence ID" value="AEF96615"/>
        <property type="gene ID" value="Metig_1076"/>
    </dbReference>
    <dbReference type="GeneID" id="10643930"/>
    <dbReference type="KEGG" id="mig:Metig_1076"/>
    <dbReference type="HOGENOM" id="CLU_000604_8_0_2"/>
    <dbReference type="OMA" id="GSLYRIR"/>
    <dbReference type="OrthoDB" id="45576at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003838">
        <property type="entry name" value="ABC3_permease_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025857">
        <property type="entry name" value="MacB_PCD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02687">
        <property type="entry name" value="FtsX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12704">
        <property type="entry name" value="MacB_PCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="241"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="286"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="329"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="domain" description="MacB_PCD" evidence="8">
        <location>
            <begin position="17"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="domain" description="FtsX" evidence="7">
        <location>
            <begin position="271"/>
            <end position="360"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF12704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AEF96615.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="367" mass="40583" checksum="2A86386AC70731C9" modified="2011-07-27" version="1">MYLKMAKRNLNRHILRSTLALLGIIIGVMAISSLGILGGGLKQGIIKNFEGVANFVVVLPNTEEGYLHFTKKDVDKLKKLNCIVIPISSKTDIVYIKGKNKKAYVTIYGIKKEDIKYLNLGIKNKLTDTTTYADSFFVNIHDVNTGDMVVLKNISLRINGIYNSSFFVISQNSIILSEKTYKRFYGNNYSMIVLYVKNKDDISKIKNETEKIMNRKEKTAIVLSMDRILQSINDVMDKVSLFLMGIGGISLLVAGIGIGNVMLMSTIERTKEIGVMKSIGASKRDIITLFLYEALILGVVGSIIGAMLSLGVGYLVVHYLLRSSITMESLIYVFLGILFGVGTSIVASLYPAYKAANLDPIKALKSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDS5</accession>
    <name>F6BDS5_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Glycine--tRNA ligase</fullName>
            <ecNumber evidence="7">6.1.1.14</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Glycyl-tRNA synthetase</fullName>
            <shortName evidence="7">GlyRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">glyS</name>
        <name evidence="9" type="ordered locus">Metig_1097</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the attachment of glycine to tRNA(Gly).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-tRNA(Gly)</text>
            <dbReference type="Rhea" id="RHEA:16013"/>
            <dbReference type="Rhea" id="RHEA-COMP:9664"/>
            <dbReference type="Rhea" id="RHEA-COMP:9683"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78522"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.14"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.14" evidence="7"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96636.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799237.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1097"/>
    <dbReference type="EnsemblBacteria" id="AEF96636">
        <property type="protein sequence ID" value="AEF96636"/>
        <property type="gene ID" value="Metig_1097"/>
    </dbReference>
    <dbReference type="GeneID" id="10643955"/>
    <dbReference type="KEGG" id="mig:Metig_1097"/>
    <dbReference type="HOGENOM" id="CLU_015515_1_2_2"/>
    <dbReference type="OMA" id="EPSYGID"/>
    <dbReference type="OrthoDB" id="32623at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004820">
        <property type="term" value="F:glycine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006426">
        <property type="term" value="P:glycyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00774">
        <property type="entry name" value="GlyRS-like_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.800">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00253_A">
        <property type="entry name" value="Gly_tRNA_synth_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002314">
        <property type="entry name" value="aa-tRNA-synt_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004154">
        <property type="entry name" value="Anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036621">
        <property type="entry name" value="Anticodon-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027031">
        <property type="entry name" value="Gly-tRNA_synthase/POLG2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022960">
        <property type="entry name" value="Gly_tRNA_ligase_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033731">
        <property type="entry name" value="GlyRS-like_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002315">
        <property type="entry name" value="tRNA-synt_gly"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10745">
        <property type="entry name" value="PTHR10745"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03129">
        <property type="entry name" value="HGTP_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00587">
        <property type="entry name" value="tRNA-synt_2b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01043">
        <property type="entry name" value="TRNASYNTHGLY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="SSF55681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00389">
        <property type="entry name" value="glyS_dimeric"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7 9" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="5 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 7" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AA_TRNA_LIGASE_II" evidence="8">
        <location>
            <begin position="2"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="7">
        <location>
            <begin position="209"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="7">
        <location>
            <begin position="445"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="96"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="162"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="194"/>
            <end position="196"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="204"/>
            <end position="209"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="326"/>
            <end position="327"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="449"/>
            <end position="452"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AEF96636.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="575" mass="66740" checksum="C3106AF814C9AD48" modified="2011-07-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDW4</accession>
    <name>F6BDW4_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Type II secretion system F domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Metig_1136</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96675.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799276.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1136"/>
    <dbReference type="EnsemblBacteria" id="AEF96675">
        <property type="protein sequence ID" value="AEF96675"/>
        <property type="gene ID" value="Metig_1136"/>
    </dbReference>
    <dbReference type="GeneID" id="10643994"/>
    <dbReference type="KEGG" id="mig:Metig_1136"/>
    <dbReference type="HOGENOM" id="CLU_929424_0_0_2"/>
    <dbReference type="OMA" id="NRMIITC"/>
    <dbReference type="OrthoDB" id="60030at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.81.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018076">
        <property type="entry name" value="T2SS_GspF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042094">
        <property type="entry name" value="T2SS_GspF_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00482">
        <property type="entry name" value="T2SSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="30"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="55"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="197"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="242"/>
            <end position="261"/>
        </location>
    </feature>
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        <location>
            <begin position="268"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="domain" description="T2SSF" evidence="8">
        <location>
            <begin position="90"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00482"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AEF96675.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="295" mass="32895" checksum="44633A8934BC9163" modified="2011-07-27" version="1">MSIINKIYIYFIKRNIRILRKIGKRVDERVFISLLLFVFAIPLVFSLFFNLTLKTAIILALVYLASALSIPTILYEIKIERFEQNLPKALYTMILALESGRSLTEAMEEVIKSGISEVDKVFARVMILMINQKMSFEDAMLVVASYLDSKVFRQLSRLLIENRKYGGDLANTLKILAKTLEDFQNLRRQLLSVTANGLAVGLVILCGVIPATAALIGGYLIIVSKIAPQMPSVTQDQIAKCVEIIQTGTGIFGLLFAIPLYGLKLGRMILMCAICMTMGIIAFYGTLKLTGMLFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-06-02" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDX0</accession>
    <name>F6BDX0_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_1142</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96681.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799282.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96681">
        <property type="protein sequence ID" value="AEF96681"/>
        <property type="gene ID" value="Metig_1142"/>
    </dbReference>
    <dbReference type="GeneID" id="10644000"/>
    <dbReference type="KEGG" id="mig:Metig_1142"/>
    <dbReference type="HOGENOM" id="CLU_1551839_0_0_2"/>
    <dbReference type="OMA" id="CKHIDYL"/>
    <dbReference type="OrthoDB" id="89860at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF96681.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="172" mass="19973" checksum="30871EC039DC129B" modified="2011-07-27" version="1">MEYLPKEIISTPFRKAILHYVLIRGTTYPQQVSENLRISKGLPSQFLRLCTALGVTKRERNGHKVLYSLTTKGIAILKRLCPEIFDLSFSSIFENLSKMKFNTKYYPVEKIGFEVKKIVDNFGGVTFVFYDADGEEISRVFKPKKGSWWCISCQSSNCKHINYLKKYYDKTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDX8</accession>
    <name>F6BDX8_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">H4MPT-linked C1 transfer pathway protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1150</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96689.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1150"/>
    <dbReference type="EnsemblBacteria" id="AEF96689">
        <property type="protein sequence ID" value="AEF96689"/>
        <property type="gene ID" value="Metig_1150"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_1150"/>
    <dbReference type="HOGENOM" id="CLU_060932_0_0_2"/>
    <dbReference type="OMA" id="TDCFADR"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002821">
        <property type="entry name" value="Hydantoinase_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002756">
        <property type="entry name" value="MfnF"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01968">
        <property type="entry name" value="Hydantoinase_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="SSF53067"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03123">
        <property type="entry name" value="one_C_unchar_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Hydantoinase_A" evidence="1">
        <location>
            <begin position="51"/>
            <end position="320"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96689.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="326" mass="36595" checksum="140716940958719D" modified="2011-07-27" version="1">MILGIDIGGANTKITEIDENDYKIHHIYFPMWKNNEKLTELLKKYSENVDTVGIVMTAELVDAYETKKEGVNDILNSVENAYDCPIYVLDADGNFLTPEEARENYLKVSAANWMATAKFVSEYIDNNCILVDMGSTTTDIIPIKDGKILANKKDLDRLMNNELIYVGTLRTPLSFLANKINFRGKITHVSSEYFAITGDIHVILGKIKEEDYTCETPDGKGVDRDACLTRVARVLCGDREMINDDELTAIAEEFYAKLLKLIREGVDEVSKKYELKNVVITGLGEEILKDALKGYNIKSIKEIYGKEVSLATPSFAVGMLLKKYIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDY6</accession>
    <name>F6BDY6_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Nitrogen regulatory protein P-II</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_1158</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the P(II) protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96697.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799298.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1158"/>
    <dbReference type="EnsemblBacteria" id="AEF96697">
        <property type="protein sequence ID" value="AEF96697"/>
        <property type="gene ID" value="Metig_1158"/>
    </dbReference>
    <dbReference type="GeneID" id="10644016"/>
    <dbReference type="KEGG" id="mig:Metig_1158"/>
    <dbReference type="HOGENOM" id="CLU_082268_0_0_2"/>
    <dbReference type="OMA" id="LEMAIHT"/>
    <dbReference type="OrthoDB" id="113533at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030234">
        <property type="term" value="F:enzyme regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006808">
        <property type="term" value="P:regulation of nitrogen utilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002187">
        <property type="entry name" value="N-reg_PII"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011322">
        <property type="entry name" value="N-reg_PII-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015867">
        <property type="entry name" value="N-reg_PII/ATP_PRibTrfase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017918">
        <property type="entry name" value="N-reg_PII_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30115">
        <property type="entry name" value="PTHR30115"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00543">
        <property type="entry name" value="P-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039144">
        <property type="entry name" value="GlnB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00340">
        <property type="entry name" value="PIIGLNB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00938">
        <property type="entry name" value="P-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54913">
        <property type="entry name" value="SSF54913"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00638">
        <property type="entry name" value="PII_GLNB_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51343">
        <property type="entry name" value="PII_GLNB_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="modified residue" description="O-UMP-tyrosine" evidence="1">
        <location>
            <position position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR602187-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF96697.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="112" mass="12362" checksum="FFD54C2772AFC66A" modified="2011-07-27" version="1">MKKIEAIIRPSKLEDVKNALLKAGCKGLTVSEVKGRGVQGGIVERYRGREYIIDLLPKVKIEIVVDDANVEKIIDLICENAKTGEFGDGKIFVIPVEEVVRVRTGERGKDAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>F6BE06</accession>
    <name>F6BE06_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Phospholipase D/Transphosphatidylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_1179</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96717.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1179"/>
    <dbReference type="EnsemblBacteria" id="AEF96717">
        <property type="protein sequence ID" value="AEF96717"/>
        <property type="gene ID" value="Metig_1179"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_1179"/>
    <dbReference type="HOGENOM" id="CLU_122247_1_0_2"/>
    <dbReference type="OMA" id="GSHNWTD"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025202">
        <property type="entry name" value="PLD-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001736">
        <property type="entry name" value="PLipase_D/transphosphatidylase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13091">
        <property type="entry name" value="PLDc_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00155">
        <property type="entry name" value="PLDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50035">
        <property type="entry name" value="PLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="domain" description="PLD phosphodiesterase" evidence="2">
        <location>
            <begin position="131"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF96717.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="188" mass="22157" checksum="6C829EA75A261826" modified="2011-07-27" version="1">MNFNTFLLVMLLVISITLTFMTIIINKKIKILIQKLEDTTEEIIDEDKDIVVLNDEKYFYYVLNLIKSAKKEIDIIMFSMFRCKQTEELLNALIEARKRGVMVRIILNKEVESNKEVKNYLASERISVKYSKTRVHNKLVIVDDTVVVGSHNWTDSGLFENRESSIAIRDKNVLKKEKEYFEALWERL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>F6BE09</accession>
    <name>F6BE09_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Acetylornithine aminotransferase</fullName>
            <shortName evidence="2">ACOAT</shortName>
            <ecNumber evidence="2">2.6.1.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">argD</name>
        <name evidence="4" type="ordered locus">Metig_1182</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>2-oxoglutarate + N(2)-acetyl-L-ornithine = L-glutamate + N-acetyl-L-glutamate 5-semialdehyde</text>
            <dbReference type="Rhea" id="RHEA:18049"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29123"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57805"/>
            <dbReference type="EC" id="2.6.1.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
        <text evidence="2">Binds 1 pyridoxal phosphate per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-arginine biosynthesis; N(2)-acetyl-L-ornithine from L-glutamate: step 4/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="miscellaneous">
        <text evidence="2">May also have succinyldiaminopimelate aminotransferase activity, thus carrying out the corresponding step in lysine biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. ArgD subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.11" evidence="2"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96720.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799319.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1182"/>
    <dbReference type="EnsemblBacteria" id="AEF96720">
        <property type="protein sequence ID" value="AEF96720"/>
        <property type="gene ID" value="Metig_1182"/>
    </dbReference>
    <dbReference type="GeneID" id="10644042"/>
    <dbReference type="KEGG" id="mig:Metig_1182"/>
    <dbReference type="HOGENOM" id="CLU_016922_10_1_2"/>
    <dbReference type="OMA" id="MVPNYNP"/>
    <dbReference type="OrthoDB" id="20774at2157"/>
    <dbReference type="UniPathway" id="UPA00068">
        <property type="reaction ID" value="UER00109"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003992">
        <property type="term" value="F:N2-acetyl-L-ornithine:2-oxoglutarate 5-aminotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006526">
        <property type="term" value="P:arginine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00610">
        <property type="entry name" value="OAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01107">
        <property type="entry name" value="ArgD_aminotrans_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004636">
        <property type="entry name" value="AcOrn/SuccOrn_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005814">
        <property type="entry name" value="Aminotrans_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00202">
        <property type="entry name" value="Aminotran_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000521">
        <property type="entry name" value="Transaminase_4ab_Lys_Orn"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00707">
        <property type="entry name" value="argD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00600">
        <property type="entry name" value="AA_TRANSFER_CLASS_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="2 4" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="2" id="KW-0055">Arginine biosynthesis</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1 2 3" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Pyridoxal phosphate binding" evidence="2">
        <location>
            <begin position="106"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Pyridoxal phosphate binding" evidence="2">
        <location>
            <begin position="224"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="139"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="142"/>
        </location>
        <ligand>
            <name>N(2)-acetyl-L-ornithine</name>
            <dbReference type="ChEBI" id="CHEBI:57805"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="281"/>
        </location>
        <ligand>
            <name>N(2)-acetyl-L-ornithine</name>
            <dbReference type="ChEBI" id="CHEBI:57805"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="282"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="2">
        <location>
            <position position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEF96720.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="398" mass="44069" checksum="EDA5B8AC8069A989" modified="2011-07-27" version="1">MQEMEKYIEEEKKYHLQTYGRLPVVLVKGEGMYVYDVNGKKYVDFLAGIGVNNVGHCHPKVVEAIKKQAETLIHTSNIYYTIPQIELAKKLVNLSGLNRAFFCNSGAEANEAAIKLARKYAKEILGKDGGEIITMENAFHGRTITTITATPKPKYQKGYEPLTPGFKYVPFNDIEALKESITDKTVAIMLEPVQGEGGIHVADKEYLRAVRDLCDDKNLVLIFDEVQCGMGRTGKMFAFEHYGVKPDILTLAKALGGGVPIGAMLAKEEIAKAMGYGDHGTTFGGNPLACSAALATIEVIEGILGHVEEMGKYFMDKLETLNKKYPFIKEVRGLGLMLGMELEFNGGEIVKKMLEKGYLINCTSDVVLRFLPPLIVEKEHIDGLIKALDEVFSEMNNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>F6BE10</accession>
    <name>F6BE10_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Coenzyme F420:L-glutamate ligase</fullName>
            <ecNumber evidence="8">6.3.2.31</ecNumber>
            <ecNumber evidence="8">6.3.2.34</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Coenzyme F420-0:L-glutamate ligase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Coenzyme F420-1:gamma-L-glutamate ligase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">cofE</name>
        <name evidence="10" type="ordered locus">Metig_1183</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the GTP-dependent successive addition of two or more gamma-linked L-glutamates to the L-lactyl phosphodiester of 7,8-didemethyl-8-hydroxy-5-deazariboflavin (F420-0) to form coenzyme F420-0-glutamyl-glutamate (F420-2) or polyglutamated F420 derivatives.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>GTP + L-glutamate + oxidized coenzyme F420-0 = GDP + H(+) + oxidized coenzyme F420-1 + phosphate</text>
            <dbReference type="Rhea" id="RHEA:30555"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="ChEBI" id="CHEBI:59907"/>
            <dbReference type="ChEBI" id="CHEBI:59920"/>
            <dbReference type="EC" id="6.3.2.31"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>GTP + L-glutamate + oxidized coenzyme F420-1 = GDP + H(+) + oxidized coenzyme F420-2 + phosphate</text>
            <dbReference type="Rhea" id="RHEA:30523"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57922"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="ChEBI" id="CHEBI:59920"/>
            <dbReference type="EC" id="6.3.2.34"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </cofactor>
        <text evidence="8">Monovalent cation. The ion could be potassium.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="8">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="8">Binds 2 divalent metal cations per subunit. The ions could be magnesium and/or manganese.</text>
    </comment>
    <comment type="pathway">
        <text evidence="8">Cofactor biosynthesis; coenzyme F420 biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the CofE family.</text>
    </comment>
    <dbReference type="EC" id="6.3.2.31" evidence="8"/>
    <dbReference type="EC" id="6.3.2.34" evidence="8"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96721.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799320.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1183"/>
    <dbReference type="EnsemblBacteria" id="AEF96721">
        <property type="protein sequence ID" value="AEF96721"/>
        <property type="gene ID" value="Metig_1183"/>
    </dbReference>
    <dbReference type="GeneID" id="10644043"/>
    <dbReference type="KEGG" id="mig:Metig_1183"/>
    <dbReference type="HOGENOM" id="CLU_051152_1_1_2"/>
    <dbReference type="OMA" id="KHGFVCA"/>
    <dbReference type="OrthoDB" id="73785at2157"/>
    <dbReference type="UniPathway" id="UPA00071"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052618">
        <property type="term" value="F:coenzyme F420-0:L-glutamate ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052619">
        <property type="term" value="F:coenzyme F420-1:gamma-L-glutamate ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052645">
        <property type="term" value="P:F420-0 metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01258">
        <property type="entry name" value="F420_ligase_CofE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008225">
        <property type="entry name" value="F420-0_g-glutamyl_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002847">
        <property type="entry name" value="F420-0_gamma-glut_ligase-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023659">
        <property type="entry name" value="F420_ligase_CofE_arc"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01996">
        <property type="entry name" value="F420_ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01916">
        <property type="entry name" value="F420_cofE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1 8 10" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7 8" id="KW-0464">Manganese</keyword>
    <keyword evidence="2 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 8" id="KW-0630">Potassium</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F420_ligase" evidence="9">
        <location>
            <begin position="27"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="15"/>
            <end position="18"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="45"/>
            <end position="46"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="50"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="115"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="118"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="154"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="155"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="210"/>
            <end position="217"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="212"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01996"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AEF96721.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="248" mass="27231" checksum="882477876ED3E5CD" modified="2011-07-27" version="1">MIREKRKVEVIGLELPIFKGNEHVNLAELIAQYPLEDGDVIVIAETLISKVEGNILKKEDIKPSKEAIELAKKLGKEPEVMHVILDEAKEIVKIGKNFIITETKHGFVCANSGVDESNVDGIKPLPKNPDESAEKIRKEIEKITKKKVGVIISDSVGRPFRKGAVGVAIGVSGILALWDRKGEKDLFGRELRTTEVAIADELASMANVVMGEANEGIPVVIIRNAPVPFGNGKGKDLIRKKEEDVFRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2020-12-02" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>F6BE53</accession>
    <name>F6BE53_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0028</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95589.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF95589">
        <property type="protein sequence ID" value="AEF95589"/>
        <property type="gene ID" value="Metig_0028"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0028"/>
    <dbReference type="HOGENOM" id="CLU_1021630_0_0_2"/>
    <dbReference type="OMA" id="FMVKWNG"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005358">
        <property type="entry name" value="Puta_zinc/iron-chelating_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03692">
        <property type="entry name" value="CxxCxxCC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="190"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF95589.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="278" mass="33473" checksum="46668AC3F398FAD0" modified="2011-07-27" version="1">MEWEITFDGITYECINCAYCCSCKDWRIYLDYFDVLKLKDYNYAIEDCERGKFKKRLKVGGEGCVLLHNNLCKIHLEKGYEYKPLMCKIFPFSFMVKWNGDLLLIIKHYCKGIKKGKCDKRMIKKAMEYCEELYLDELDDIIFEAMEHSSKTKIDDNWRVSWEEREEFGRNIFKSKNLNEVAEKCKELINYDVSERINKIKDNLSKLEEDIRKENEEEIIRFMGELNKREHFRKFPFKKEIDKLLEIGERISRYKNVFKGEAIIDKELLLNPNSHYTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>F6BE90</accession>
    <name>F6BE90_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Flavin prenyltransferase UbiX</fullName>
            <ecNumber evidence="5">2.5.1.129</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">ubiX</name>
        <name evidence="7" type="ordered locus">Metig_1230</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Flavin prenyltransferase that catalyzes the synthesis of the prenylated FMN cofactor (prenyl-FMN) for 4-hydroxy-3-polyprenylbenzoic acid decarboxylase UbiD. The prenyltransferase is metal-independent and links a dimethylallyl moiety from dimethylallyl monophosphate (DMAP) to the flavin N5 and C6 atoms of FMN.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>dimethylallyl phosphate + FMNH2 = phosphate + prenyl-FMNH2</text>
            <dbReference type="Rhea" id="RHEA:37743"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57618"/>
            <dbReference type="ChEBI" id="CHEBI:87467"/>
            <dbReference type="ChEBI" id="CHEBI:88052"/>
            <dbReference type="EC" id="2.5.1.129"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the UbiX/PAD1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.129" evidence="5"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96767.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799366.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1230"/>
    <dbReference type="EnsemblBacteria" id="AEF96767">
        <property type="protein sequence ID" value="AEF96767"/>
        <property type="gene ID" value="Metig_1230"/>
    </dbReference>
    <dbReference type="GeneID" id="10644091"/>
    <dbReference type="KEGG" id="mig:Metig_1230"/>
    <dbReference type="HOGENOM" id="CLU_074522_0_1_2"/>
    <dbReference type="OMA" id="GATHIQD"/>
    <dbReference type="OrthoDB" id="112962at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106141">
        <property type="term" value="F:flavin prenyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1950">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01984">
        <property type="entry name" value="ubiX_pad"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036551">
        <property type="entry name" value="Flavin_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003382">
        <property type="entry name" value="Flavoprotein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004507">
        <property type="entry name" value="UbiX-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43374">
        <property type="entry name" value="PTHR43374"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02441">
        <property type="entry name" value="Flavoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52507">
        <property type="entry name" value="SSF52507"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00421">
        <property type="entry name" value="ubiX_pad"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3 5" id="KW-0288">FMN</keyword>
    <keyword evidence="7" id="KW-0456">Lyase</keyword>
    <keyword evidence="1 5" id="KW-0637">Prenyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Flavoprotein" evidence="6">
        <location>
            <begin position="1"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="9"/>
            <end position="11"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="35"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="86"/>
            <end position="89"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="121"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="151"/>
        </location>
        <ligand>
            <name>dimethylallyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:88052"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="167"/>
        </location>
        <ligand>
            <name>dimethylallyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:88052"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEF96767.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="184" mass="20681" checksum="312C340B2B6AFB2B" modified="2011-07-27" version="1">MKVVVCVTGASGAIYAKRLLEVLKEKNVETFLIISNSAKKIIEHELKIKCNDFIKLADKYYENDDFFSPLASGSNKFDAAIVIPCSMKTLSSIANGYSSNLIVRVCDIALKEKRKLILMPREMPFNAIHLENMLKLAKLGVVIMPPIPAFYNEPKTIDDLVNFVVGRVLDILGIENDLFKRWGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>F6BE95</accession>
    <name>F6BE95_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Methyl-coenzyme M reductase operon protein C</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metig_1235</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="2 3">MCR is composed of three subunits: alpha, beta, and gamma. The function of proteins C and D is not known.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96772.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799371.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1235"/>
    <dbReference type="EnsemblBacteria" id="AEF96772">
        <property type="protein sequence ID" value="AEF96772"/>
        <property type="gene ID" value="Metig_1235"/>
    </dbReference>
    <dbReference type="GeneID" id="10644098"/>
    <dbReference type="KEGG" id="mig:Metig_1235"/>
    <dbReference type="HOGENOM" id="CLU_099719_0_0_2"/>
    <dbReference type="OMA" id="PVCEITF"/>
    <dbReference type="OrthoDB" id="65557at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015948">
        <property type="term" value="P:methanogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007687">
        <property type="entry name" value="Me_CoM_Rdtase_prot-C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026327">
        <property type="entry name" value="Me_CoM_Rdtase_prot-C-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04609">
        <property type="entry name" value="MCR_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003137">
        <property type="entry name" value="McrC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03264">
        <property type="entry name" value="met_CoM_red_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0484">Methanogenesis</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00025920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRNR" id="PIRNR003137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEF96772.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="198" mass="21454" checksum="BB696578F266E640" modified="2011-07-27" version="1">MPVGRKEQIIDCRAVMGLGEGGGLAQRGTFAEALRNDVVVVAMSPGRRHITKPVCEITYGIREAGIQTSVLVLDAGSGIPHDAPHGSLGSTFGLKPEEAAQINRHKLCVVHFGNVINHVVYKARLLLRYVEIPTIVVCQCPVDMEDFAKVGVKTLNVMPVEPQTKGMIVDIVTGVVRGESCPQTKIDEIIEKIKKHLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>F6BE97</accession>
    <name>F6BE97_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Methyl-coenzyme M reductase subunit alpha</fullName>
            <ecNumber evidence="11">2.8.4.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ordered locus">Metig_1237</name>
    </gene>
    <organism evidence="17">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 11">Component of the methyl-coenzyme M reductase (MCR) I that catalyzes the reductive cleavage of methyl-coenzyme M (CoM-S-CH3 or 2-(methylthio)ethanesulfonate) using coenzyme B (CoB or 7-mercaptoheptanoylthreonine phosphate) as reductant which results in the production of methane and the mixed heterodisulfide of CoB and CoM (CoM-S-S-CoB). This is the final step in methanogenesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>coenzyme B + methyl-coenzyme M = coenzyme M-coenzyme B heterodisulfide + methane</text>
            <dbReference type="Rhea" id="RHEA:12532"/>
            <dbReference type="ChEBI" id="CHEBI:16183"/>
            <dbReference type="ChEBI" id="CHEBI:58286"/>
            <dbReference type="ChEBI" id="CHEBI:58411"/>
            <dbReference type="ChEBI" id="CHEBI:58596"/>
            <dbReference type="EC" id="2.8.4.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="1">
            <dbReference type="Rhea" id="RHEA:12533"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>coenzyme F430</name>
            <dbReference type="ChEBI" id="CHEBI:60540"/>
        </cofactor>
        <text evidence="11">Binds 2 coenzyme F430 non-covalently per MCR complex. Coenzyme F430 is a yellow nickel porphinoid. Methyl-coenzyme-M reductase is activated when the enzyme-bound coenzyme F430 is reduced to the Ni(I) oxidation state.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3 11">One-carbon metabolism; methyl-coenzyme M reduction; methane from methyl-coenzyme M: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Hexamer of two alpha, two beta, and two gamma chains.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">MCR is a hexamer of two alpha, two beta, and two gamma chains, forming a dimer of heterotrimers.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the methyl-coenzyme M reductase alpha subunit family.</text>
    </comment>
    <dbReference type="EC" id="2.8.4.1" evidence="11"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96774.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799373.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1237"/>
    <dbReference type="EnsemblBacteria" id="AEF96774">
        <property type="protein sequence ID" value="AEF96774"/>
        <property type="gene ID" value="Metig_1237"/>
    </dbReference>
    <dbReference type="GeneID" id="10644100"/>
    <dbReference type="KEGG" id="mig:Metig_1237"/>
    <dbReference type="HOGENOM" id="CLU_493170_0_0_2"/>
    <dbReference type="OMA" id="AMLYDQI"/>
    <dbReference type="OrthoDB" id="6589at2157"/>
    <dbReference type="UniPathway" id="UPA00646">
        <property type="reaction ID" value="UER00699"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050524">
        <property type="term" value="F:coenzyme-B sulfoethylthiotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015948">
        <property type="term" value="P:methanogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.840.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.470">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.390.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016212">
        <property type="entry name" value="Me_CoM_Rdtase_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008924">
        <property type="entry name" value="Me_CoM_Rdtase_asu/bsu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009047">
        <property type="entry name" value="Me_CoM_Rdtase_asu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003183">
        <property type="entry name" value="Me_CoM_Rdtase_asu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015811">
        <property type="entry name" value="Me_CoM_Rdtase_asu_N_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015823">
        <property type="entry name" value="Me_CoM_Rdtase_asu_N_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009024">
        <property type="entry name" value="Me_CoM_Rdtase_Fd-like_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02249">
        <property type="entry name" value="MCR_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02745">
        <property type="entry name" value="MCR_alpha_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000262">
        <property type="entry name" value="MCR_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48081">
        <property type="entry name" value="SSF48081"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55088">
        <property type="entry name" value="SSF55088"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03256">
        <property type="entry name" value="met_CoM_red_alp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 11 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 11" id="KW-0484">Methanogenesis</keyword>
    <keyword evidence="6" id="KW-0488">Methylation</keyword>
    <keyword evidence="7 11 12" id="KW-0533">Nickel</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 11 16" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="MCR_alpha_N" evidence="15">
        <location>
            <begin position="5"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="domain" description="MCR_alpha" evidence="14">
        <location>
            <begin position="318"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="150"/>
        </location>
        <ligand>
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="Pros-methylhistidine" evidence="13">
        <location>
            <position position="260"/>
        </location>
    </feature>
    <feature type="modified residue" description="5-methylarginine" evidence="13">
        <location>
            <position position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000951"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR000262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR000262-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR000262-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF02249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF02745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="AEF96774.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="553" mass="61181" checksum="073CF929AED1B128" modified="2011-07-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEC6</accession>
    <name>F6BEC6_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">ATP-dependent dethiobiotin synthetase BioD</fullName>
            <ecNumber evidence="5">6.3.3.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">DTB synthetase</fullName>
            <shortName evidence="5">DTBS</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">Dethiobiotin synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">bioD</name>
        <name evidence="7" type="ordered locus">Metig_1266</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes a mechanistically unusual reaction, the ATP-dependent insertion of CO2 between the N7 and N8 nitrogen atoms of 7,8-diaminopelargonic acid (DAPA, also called 7,8-diammoniononanoate) to form a ureido ring.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>(7R,8S)-7,8-diammoniononanoate + ATP + CO2 = (4R,5S)-dethiobiotin + ADP + 3 H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:15805"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:149469"/>
            <dbReference type="ChEBI" id="CHEBI:149473"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.3.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 5">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="5">Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the dethiobiotin synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="6.3.3.3" evidence="5"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96803.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799401.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1266"/>
    <dbReference type="EnsemblBacteria" id="AEF96803">
        <property type="protein sequence ID" value="AEF96803"/>
        <property type="gene ID" value="Metig_1266"/>
    </dbReference>
    <dbReference type="GeneID" id="10644129"/>
    <dbReference type="KEGG" id="mig:Metig_1266"/>
    <dbReference type="HOGENOM" id="CLU_072551_3_1_2"/>
    <dbReference type="OMA" id="SPHWAAE"/>
    <dbReference type="OrthoDB" id="82623at2157"/>
    <dbReference type="UniPathway" id="UPA00078">
        <property type="reaction ID" value="UER00161"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004141">
        <property type="term" value="F:dethiobiotin synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009102">
        <property type="term" value="P:biotin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00336">
        <property type="entry name" value="BioD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004472">
        <property type="entry name" value="DTB_synth_BioD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43210">
        <property type="entry name" value="PTHR43210"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006755">
        <property type="entry name" value="DTB_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00347">
        <property type="entry name" value="bioD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 5" id="KW-0093">Biotin biosynthesis</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0436">Ligase</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="78"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="35"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="10"/>
            <end position="15"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="14"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="39"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="44"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="109"/>
            <end position="112"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="109"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="169"/>
            <end position="170"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEF96803.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="214" mass="24059" checksum="82713F1C0F349E97" modified="2011-07-27" version="1">MIFVTGTDTGVGKTYISSILAENLKKMGVNVGYLKPIETGGREDTLTLKNILKTDDDLDLMNPINLKLPLSPNIAFEVENYDISLDEIKEKIKNAYETLKERHDFLIVEGAGGVCVPIKENFLMSDLIKFLDLDAVVVSRPNLGTINHTLLTVEHLRNKGINVRGVIINCITDLSEVLYYEKTFETIEKVGNIEIIGIVKSREDFEIDFKKILK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEE5</accession>
    <name>F6BEE5_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0201 protein Metig_0045</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0045</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0201 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95606.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798215.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0045"/>
    <dbReference type="EnsemblBacteria" id="AEF95606">
        <property type="protein sequence ID" value="AEF95606"/>
        <property type="gene ID" value="Metig_0045"/>
    </dbReference>
    <dbReference type="GeneID" id="10642880"/>
    <dbReference type="KEGG" id="mig:Metig_0045"/>
    <dbReference type="HOGENOM" id="CLU_134829_0_0_2"/>
    <dbReference type="OMA" id="VHKQVAY"/>
    <dbReference type="OrthoDB" id="92838at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1440.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01112">
        <property type="entry name" value="UPF0201"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022803">
        <property type="entry name" value="Ribosomal_L5_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002739">
        <property type="entry name" value="UPF0201"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39652">
        <property type="entry name" value="PTHR39652"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01877">
        <property type="entry name" value="RNA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55282">
        <property type="entry name" value="SSF55282"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF95606.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="148" mass="17062" checksum="A62AFFCAB084DF2D" modified="2011-07-27" version="1">MIVKIKAKVKPTEDKGKVKRAILKIFPKAELEFNEEYNYWEGETKNVDRFKELLRSQAILDAARVVLEKGIVGDYTKFFINKQAAYVGSLNFDRDVHGGIEVKIIAEENEDLMKIIKDIAPRTKNGVIINEDEEMEENEEETKEEANN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEF3</accession>
    <name>F6BEF3_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Putative adenylate kinase</fullName>
            <shortName evidence="2">AK</shortName>
            <ecNumber evidence="2">2.7.4.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">ATP-AMP transphosphorylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_0053</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Broad-specificity nucleoside monophosphate (NMP) kinase that catalyzes the reversible transfer of the terminal phosphate group between nucleoside triphosphates and monophosphates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 2">
            <text>AMP + ATP = 2 ADP</text>
            <dbReference type="Rhea" id="RHEA:12973"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.3"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the adenylate kinase family. AK6 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.3" evidence="2"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95614.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0053"/>
    <dbReference type="EnsemblBacteria" id="AEF95614">
        <property type="protein sequence ID" value="AEF95614"/>
        <property type="gene ID" value="Metig_0053"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0053"/>
    <dbReference type="HOGENOM" id="CLU_079096_0_1_2"/>
    <dbReference type="OMA" id="QCEIFGT"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004017">
        <property type="term" value="F:adenylate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00039">
        <property type="entry name" value="Adenylate_kinase_AK6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020618">
        <property type="entry name" value="Adenyl_kinase_AK6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12595">
        <property type="entry name" value="PTHR12595"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 3" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="NMP" evidence="2">
        <location>
            <begin position="33"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="region of interest" description="LID" evidence="2">
        <location>
            <begin position="102"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="13"/>
            <end position="18"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="99"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="103"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF95614.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="175" mass="20269" checksum="4CBFD251EA60F6EA" modified="2011-07-27" version="1">MRNIKIAITGTPGVGKTTISKKLAEKLGFKHIDITEVVKKHKLYSEKDEEMDSYVIDFDKLREFLDGLDNIILDGHVSHLLDADYIVVLRCNPETVKKRLEERGYKQKKVMENVEAEILDVCLAESEGMVYEIDTTGRDVEDIVDEIIDAIKNKKIRKGIVDWTEKYFYLLEQFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEI6</accession>
    <name>F6BEI6_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">NTP_transf_3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0086</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95647.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0086"/>
    <dbReference type="EnsemblBacteria" id="AEF95647">
        <property type="protein sequence ID" value="AEF95647"/>
        <property type="gene ID" value="Metig_0086"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0086"/>
    <dbReference type="HOGENOM" id="CLU_098907_1_0_2"/>
    <dbReference type="OMA" id="GMGEKPC"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005245">
        <property type="entry name" value="CHP00454"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025877">
        <property type="entry name" value="MobA-like_NTP_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12804">
        <property type="entry name" value="NTP_transf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00454">
        <property type="entry name" value="TIGR00454"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NTP_transf_3" evidence="1">
        <location>
            <begin position="1"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF95647.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="189" mass="21290" checksum="1BC2BF14B4CE1AF7" modified="2011-07-27" version="1">MAGGKGSRMGYVEKPLIEINGKYMIDYVVEALLQSNVEKIFVAVSKNTPKTEQYIKEKYDEIKDIILIKTSGNGYVEDLNQAIKYFSSPFLVISSDIPTIKPKTINNIINYYLHIKGINNKVEALCVMVKKDNYPGNPTMEIKGYIPAGINIISPKSGEQIEEAMVVDEVLINVNTWEDKVLVEKLLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEM5</accession>
    <name>F6BEM5_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Probable L-tyrosine/L-aspartate decarboxylase</fullName>
            <shortName evidence="4">TDC/ADC</shortName>
            <ecNumber evidence="4">4.1.1.11</ecNumber>
            <ecNumber evidence="4">4.1.1.25</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">mfnA</name>
        <name evidence="6" type="ordered locus">Metig_1285</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Catalyzes the decarboxylation of L-tyrosine to produce tyramine for methanofuran biosynthesis. Can also catalyze the decarboxylation of L-aspartate to produce beta-alanine for coenzyme A (CoA) biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>H(+) + L-aspartate = beta-alanine + CO2</text>
            <dbReference type="Rhea" id="RHEA:19497"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:57966"/>
            <dbReference type="EC" id="4.1.1.11"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>H(+) + L-tyrosine = CO2 + tyramine</text>
            <dbReference type="Rhea" id="RHEA:14345"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:58315"/>
            <dbReference type="ChEBI" id="CHEBI:327995"/>
            <dbReference type="EC" id="4.1.1.25"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 4 5">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4">Cofactor biosynthesis; coenzyme A biosynthesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4">Cofactor biosynthesis; methanofuran biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the group II decarboxylase family. MfnA subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.11" evidence="4"/>
    <dbReference type="EC" id="4.1.1.25" evidence="4"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96822.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1285"/>
    <dbReference type="EnsemblBacteria" id="AEF96822">
        <property type="protein sequence ID" value="AEF96822"/>
        <property type="gene ID" value="Metig_1285"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_1285"/>
    <dbReference type="HOGENOM" id="CLU_028929_2_1_2"/>
    <dbReference type="OMA" id="DPHKMGL"/>
    <dbReference type="UniPathway" id="UPA00080"/>
    <dbReference type="UniPathway" id="UPA00241"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004068">
        <property type="term" value="F:aspartate 1-decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004837">
        <property type="term" value="F:tyrosine decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019752">
        <property type="term" value="P:carboxylic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015937">
        <property type="term" value="P:coenzyme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001120">
        <property type="term" value="P:methanofuran biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01610">
        <property type="entry name" value="MfnA_decarbox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020931">
        <property type="entry name" value="MfnA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002129">
        <property type="entry name" value="PyrdxlP-dep_de-COase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021115">
        <property type="entry name" value="Pyridoxal-P_BS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00282">
        <property type="entry name" value="Pyridoxal_deC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03812">
        <property type="entry name" value="tyr_de_CO2_Arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00392">
        <property type="entry name" value="DDC_GAD_HDC_YDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="3 4 6" id="KW-0456">Lyase</keyword>
    <keyword evidence="4 5" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="4 5">
        <location>
            <position position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01610"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR602129-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEF96822.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="383" mass="43224" checksum="A1515D127E9BFD5A" modified="2011-07-27" version="1">MEEKGISEREVLEALKKYREMDLKYENGRILGSMCTKPHPISKKIVEMFLETNLGDPGLFKGTKKLEEEVIGMIGELLHNKNAFGYIITGGTEANLTAMRAIKNMKNKNAKIIIPETAHFSFDKARDMMDLEFIKAPITKDYTIDVDFVRDYVEDYKVDGIVGIAGSTELGTIDNIEELSKIAVENDIYLHVDAAFGGFVIPFLDERYKKKNINYKFDFSLEGVCSITIDPHKMGLSPIPAGGILFRDKSFKKYLNIEAPYLTETQQATIVGTRAGFSVACTWGIMKLLGKEGYKKIVSECMENTIYLTKKAKEYGIESVIEPVMNIVALKDENPKETCSKLKKHGWYVSICKCVNALRIVVMPHVKKEHIDEFIEVLVSLKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEQ4</accession>
    <name>F6BEQ4_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Aspartokinase</fullName>
            <ecNumber evidence="7">2.7.2.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Metig_1314</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>ATP + L-aspartate = 4-phospho-L-aspartate + ADP</text>
            <dbReference type="Rhea" id="RHEA:23776"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57535"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.2.4"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="8">Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; (S)-tetrahydrodipicolinate from L-aspartate: step 1/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="8">Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-homoserine from L-aspartate: step 1/3.</text>
    </comment>
    <comment type="pathway">
        <text evidence="8">Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 1/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the aspartokinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.2.4" evidence="7"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96851.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1314"/>
    <dbReference type="EnsemblBacteria" id="AEF96851">
        <property type="protein sequence ID" value="AEF96851"/>
        <property type="gene ID" value="Metig_1314"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_1314"/>
    <dbReference type="HOGENOM" id="CLU_009116_6_0_2"/>
    <dbReference type="OMA" id="DMIVQTI"/>
    <dbReference type="UniPathway" id="UPA00034">
        <property type="reaction ID" value="UER00015"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00050">
        <property type="reaction ID" value="UER00461"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00051">
        <property type="reaction ID" value="UER00462"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004072">
        <property type="term" value="F:aspartate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009089">
        <property type="term" value="P:lysine biosynthetic process via diaminopimelate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009088">
        <property type="term" value="P:threonine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd04244">
        <property type="entry name" value="AAK_AK-LysC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1160.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036393">
        <property type="entry name" value="AceGlu_kinase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002912">
        <property type="entry name" value="ACT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041746">
        <property type="entry name" value="AK-LysC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001048">
        <property type="entry name" value="Asp/Glu/Uridylate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005260">
        <property type="entry name" value="Asp_kin_monofn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001341">
        <property type="entry name" value="Asp_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018042">
        <property type="entry name" value="Aspartate_kinase_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00696">
        <property type="entry name" value="AA_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01842">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000726">
        <property type="entry name" value="Asp_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53633">
        <property type="entry name" value="SSF53633"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="SSF55021"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00656">
        <property type="entry name" value="asp_kin_monofn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00657">
        <property type="entry name" value="asp_kinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51671">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00324">
        <property type="entry name" value="ASPARTOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 7 10" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7 10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="ACT" evidence="9">
        <location>
            <begin position="369"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="domain" description="ACT" evidence="9">
        <location>
            <begin position="447"/>
            <end position="510"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AEF96851.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="510" mass="55714" checksum="E14D886EF4747F5A" modified="2011-07-27" version="1">MINSCALENLAKSRGFCRIIFKIEQLQLTTKLCFAVYIKDYLVNIMVVVMKFGGTSVGDGKRIRHVANIVLNKKKKENKDVVVVVSAMSQVTNSLVDISKEALDIKDIERINKFIKDTREKHYKAIEDAIKSEEIRNEVKKVIDERIEELEKVLIGVAYLGELTPKSKDYILSFGERLSAPILSGAIRDLGEKSVFLTGGEAGIITDDNFGCAKVVKLEVKEKLLPLLEKGYIPVVTGFIASTEDGRITTFGRGGSDYSAAIIGYGLDAEIIEIWTDVSGILTTDPKIVKNARRIPKISYIEAMELAYFGAKVLHPRTIEPAMEKGIPILVKNTFEPENEGTLITNDLEMSNSIVKAITTIKNVALINIFGAGMVGVSGTAARIFKALGKANANVILISQGSSETNVSIVVDAEDVEKSVYELKKEFENSGLIRDINVDENVCVISVVGAGMRGSKGIAGKLFTAVAESGANIKMIAQGSSEVNISFVIDEKDLIPCVKKLHKTFIENVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>F6BES5</accession>
    <name>F6BES5_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">L-fuculose phosphate aldolase</fullName>
            <ecNumber evidence="4">4.1.2.17</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">L-fuculose-1-phosphate aldolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Metig_1335</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>L-fuculose 1-phosphate = (S)-lactaldehyde + dihydroxyacetone phosphate</text>
            <dbReference type="Rhea" id="RHEA:12933"/>
            <dbReference type="ChEBI" id="CHEBI:18041"/>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
            <dbReference type="ChEBI" id="CHEBI:57846"/>
            <dbReference type="EC" id="4.1.2.17"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Cofactor biosynthesis; coenzyme F420 biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homotetramer.</text>
    </comment>
    <dbReference type="EC" id="4.1.2.17" evidence="4"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96872.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799469.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1335"/>
    <dbReference type="EnsemblBacteria" id="AEF96872">
        <property type="protein sequence ID" value="AEF96872"/>
        <property type="gene ID" value="Metig_1335"/>
    </dbReference>
    <dbReference type="GeneID" id="10644198"/>
    <dbReference type="KEGG" id="mig:Metig_1335"/>
    <dbReference type="HOGENOM" id="CLU_006033_3_1_2"/>
    <dbReference type="OMA" id="ICRYGRS"/>
    <dbReference type="OrthoDB" id="101802at2157"/>
    <dbReference type="UniPathway" id="UPA00071"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008738">
        <property type="term" value="F:L-fuculose-phosphate aldolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.225.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001303">
        <property type="entry name" value="Aldolase_II/adducin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036409">
        <property type="entry name" value="Aldolase_II/adducin_N_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00596">
        <property type="entry name" value="Aldolase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01007">
        <property type="entry name" value="Aldolase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53639">
        <property type="entry name" value="SSF53639"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0456">Lyase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldolase_II" evidence="7">
        <location>
            <begin position="4"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00020613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00030385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM01007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEF96872.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="182" mass="20455" checksum="2B72D8B096A40686" modified="2011-07-27" version="1">MDAKLFVDICHKLYERKYLVGSGGNVSVREEDLVYVTPTGSILGFLKEEDICVVNIDGEVIKGKPTSEINMHLNLYRKREDINAIIHAHSLYSTAFSIADKKIELLTPEAEIFLKEIGYVDYFEAGSLKLAEEVAKRDEDVIILKNHGVVCLGKDLIDAYIKIEVLEEVAKLNLIVNTLSQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEW2</accession>
    <name>F6BEW2_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 14">S-adenosylmethionine synthase</fullName>
            <shortName evidence="14">AdoMet synthase</shortName>
            <ecNumber evidence="6 14">2.5.1.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13 14">Methionine adenosyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">mat</name>
        <name evidence="16" type="ordered locus">Metig_0138</name>
    </gene>
    <organism evidence="17">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3 14">Catalyzes the formation of S-adenosylmethionine from methionine and ATP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 14">
            <text>ATP + H2O + L-methionine = diphosphate + phosphate + S-adenosyl-L-methionine</text>
            <dbReference type="Rhea" id="RHEA:21080"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="EC" id="2.5.1.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 14">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4 14">Amino-acid biosynthesis; S-adenosyl-L-methionine biosynthesis; S-adenosyl-L-methionine from L-methionine: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5 14">Belongs to the AdoMet synthase 2 family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.6" evidence="6 14"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95698.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798307.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="SMR" id="F6BEW2"/>
    <dbReference type="STRING" id="880724.Metig_0138"/>
    <dbReference type="EnsemblBacteria" id="AEF95698">
        <property type="protein sequence ID" value="AEF95698"/>
        <property type="gene ID" value="Metig_0138"/>
    </dbReference>
    <dbReference type="GeneID" id="10642973"/>
    <dbReference type="KEGG" id="mig:Metig_0138"/>
    <dbReference type="HOGENOM" id="CLU_057642_0_0_2"/>
    <dbReference type="OMA" id="IGHPDSI"/>
    <dbReference type="OrthoDB" id="21658at2157"/>
    <dbReference type="UniPathway" id="UPA00315">
        <property type="reaction ID" value="UER00080"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004478">
        <property type="term" value="F:methionine adenosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006556">
        <property type="term" value="P:S-adenosylmethionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.280">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00136">
        <property type="entry name" value="S_AdoMet_synth2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027790">
        <property type="entry name" value="AdoMet_synthase_2_family"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042544">
        <property type="entry name" value="AdoMet_synthase_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002795">
        <property type="entry name" value="S-AdoMet_synthetase_arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36697">
        <property type="entry name" value="PTHR36697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01941">
        <property type="entry name" value="AdoMet_Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="15" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="12 14" id="KW-0460">Magnesium</keyword>
    <keyword evidence="10 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8 14" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 14 16" id="KW-0808">Transferase</keyword>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="223"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="141"/>
            <end position="146"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00003775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00009691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00020319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00032151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="AEF95698.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="406" mass="45202" checksum="1DC67B29231B644F" modified="2011-07-27" version="1">MRNIIVKKLDLPSIEERPVEIVERKGLGHPDSICDGIAESVSRALCKMYKEKFGTVLHHNTDQVELVGGHAYPKFGGGEMVGPIYILLSGRATMEIHDKEKNEVIKLPVSTVAVKAAKEYLKKVLRNADIDKDVIVDCRIGQGSVDLVDVFERQKNEVPLANDTSFGVGYAPLSTTEKLVLEIEKYLNSEELKKELPAVGEDIKVMGLREGKKITLTIAMAVVDKYVKNVEEYKKVIEEVRKRVEKIAKEIAKDYEVEVCINTADDYERESVFLTVIGTSAEMGDDGSVGRGNRVNGLITPFRPMSMEAASGKNPINHVGKIYNILANLIANDIAKLDGVKECYVRILSQIGKPIDQPKALDIEIITEEGYKLEDIEPKAKEIANKWLDNIMEVTEMIIEGKVTTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>F6BF26</accession>
    <name>F6BF26_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcriptional regulator, XRE family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1361</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96896.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799493.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1361"/>
    <dbReference type="EnsemblBacteria" id="AEF96896">
        <property type="protein sequence ID" value="AEF96896"/>
        <property type="gene ID" value="Metig_1361"/>
    </dbReference>
    <dbReference type="GeneID" id="10644225"/>
    <dbReference type="KEGG" id="mig:Metig_1361"/>
    <dbReference type="HOGENOM" id="CLU_129113_0_0_2"/>
    <dbReference type="OMA" id="REYSATS"/>
    <dbReference type="OrthoDB" id="99300at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016472">
        <property type="entry name" value="Tscrpt_reg_MJ0621_prd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01381">
        <property type="entry name" value="HTH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005978">
        <property type="entry name" value="HTH_MJ0621_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00530">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH cro/C1-type" evidence="1">
        <location>
            <begin position="21"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96896.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="171" mass="19493" checksum="05CA6A32ED07DD7F" modified="2011-07-27" version="1">MKAHERLLLKLESSKEFIEELKRILLELNIPLKEFSEISGIPYSTLYKIMQGKDFRISTLRKIVETIKRFEQDETCEDVIAIIAARPALDRIRSKKVKIKGREYILREYPANTLEDCIIAAIQAEREGVKAIVCAPIVSASIEKVVRIPVAVIIPEKDAFMNALEVVVDKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>F6BF35</accession>
    <name>F6BF35_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Probable porphobilinogen deaminase</fullName>
            <shortName evidence="4">PBG</shortName>
            <ecNumber evidence="4">2.5.1.61</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Hydroxymethylbilane synthase</fullName>
            <shortName evidence="4">HMBS</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">Pre-uroporphyrinogen synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">hemC</name>
        <name evidence="7" type="ordered locus">Metig_1370</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Tetrapolymerization of the monopyrrole PBG into the hydroxymethylbilane pre-uroporphyrinogen in several discrete steps.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>H2O + 4 porphobilinogen = hydroxymethylbilane + 4 NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:13185"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:57845"/>
            <dbReference type="ChEBI" id="CHEBI:58126"/>
            <dbReference type="EC" id="2.5.1.61"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>dipyrromethane</name>
            <dbReference type="ChEBI" id="CHEBI:60342"/>
        </cofactor>
        <text evidence="4">Binds 1 dipyrromethane group covalently.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4">Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; coproporphyrinogen-III from 5-aminolevulinate: step 2/4.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="4">The porphobilinogen subunits are added to the dipyrromethane group.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the HMBS family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.61" evidence="4"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96905.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799501.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1370"/>
    <dbReference type="EnsemblBacteria" id="AEF96905">
        <property type="protein sequence ID" value="AEF96905"/>
        <property type="gene ID" value="Metig_1370"/>
    </dbReference>
    <dbReference type="GeneID" id="10644235"/>
    <dbReference type="KEGG" id="mig:Metig_1370"/>
    <dbReference type="HOGENOM" id="CLU_019704_1_0_2"/>
    <dbReference type="OMA" id="LWQANHI"/>
    <dbReference type="OrthoDB" id="95523at2157"/>
    <dbReference type="UniPathway" id="UPA00251">
        <property type="reaction ID" value="UER00319"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004418">
        <property type="term" value="F:hydroxymethylbilane synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006782">
        <property type="term" value="P:protoporphyrinogen IX biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00260">
        <property type="entry name" value="Porphobil_deam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000860">
        <property type="entry name" value="HemC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022417">
        <property type="entry name" value="Porphobilin_deaminase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022418">
        <property type="entry name" value="Porphobilinogen_deaminase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036803">
        <property type="entry name" value="Porphobilinogen_deaminase_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11557">
        <property type="entry name" value="PTHR11557"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01379">
        <property type="entry name" value="Porphobil_deam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03900">
        <property type="entry name" value="Porphobil_deamC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001438">
        <property type="entry name" value="4pyrrol_synth_OHMeBilane_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00151">
        <property type="entry name" value="PORPHBDMNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54782">
        <property type="entry name" value="SSF54782"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00212">
        <property type="entry name" value="hemC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Porphobil_deam" evidence="5">
        <location>
            <begin position="4"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="domain" description="Porphobil_deamC" evidence="6">
        <location>
            <begin position="223"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="modified residue" description="S-(dipyrrolylmethanemethyl)cysteine" evidence="4">
        <location>
            <position position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00260"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF03900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEF96905.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="299" mass="33584" checksum="D9BDFC419859E62E" modified="2011-07-27" version="1">MKVIIGTRGSKLALAQTYQVADLLKSEGVETEIKIIKTVGDKVQNKTLSELGIGVFTKELDLAMLNNEIDIAVHSLKDVPTVWNENLIIAATPKRESYHDLIIWNKDNEKVEEFLNGKCKAVVGTSSIRRKEFLKIMYPNIEIKLLRGNVDTRLNKLRNKEYDAIILAEAGINRLKINLNEEFNFKRLDILPSPAQGAIGIAARKDDGEIIDILKKINDKKTFLEVTAERFALREYGGGCQAPFGALAKYDEKLGKLELECAIVENGKIKRERREICCGVDDIECAKKLGMEVGRLLKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>F6BF64</accession>
    <name>F6BF64_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1399</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96934.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799530.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1399"/>
    <dbReference type="EnsemblBacteria" id="AEF96934">
        <property type="protein sequence ID" value="AEF96934"/>
        <property type="gene ID" value="Metig_1399"/>
    </dbReference>
    <dbReference type="GeneID" id="65348598"/>
    <dbReference type="KEGG" id="mig:Metig_1399"/>
    <dbReference type="HOGENOM" id="CLU_428747_0_0_2"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_repeat"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="SSF48452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="464"/>
            <end position="497"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96934.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="638" mass="75193" checksum="A513E904DBCB7805" modified="2011-07-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>F6BF81</accession>
    <name>F6BF81_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Methyltransferase type 11</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1416</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96951.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799547.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96951">
        <property type="protein sequence ID" value="AEF96951"/>
        <property type="gene ID" value="Metig_1416"/>
    </dbReference>
    <dbReference type="GeneID" id="10644289"/>
    <dbReference type="KEGG" id="mig:Metig_1416"/>
    <dbReference type="HOGENOM" id="CLU_037990_4_1_2"/>
    <dbReference type="OMA" id="VNMHLLW"/>
    <dbReference type="OrthoDB" id="49237at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013216">
        <property type="entry name" value="Methyltransf_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08241">
        <property type="entry name" value="Methyltransf_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransf_11" evidence="1">
        <location>
            <begin position="50"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96951.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="211" mass="24064" checksum="B50C62E2297B61A6" modified="2011-07-27" version="1">MNIKEYIKSRWNEHAKNYDKIPAHGVNSEKDKKVVKNALKEILKRKMKILDVGCGTGFLSLILAELGHEVVGIDLSEGMLNKAREKAKNLGLDIEFMVGDAENLPFEDNTFDAIVERHILWTLPNPKKAIKEWMRVLKDGGKIILIESESRGVNVAKHHYDDDKVIKNLPFSNGLDLERFKEIANECNLTFKVETLDCEKINLMIVCEKTC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>F6BA76</accession>
    <name>F6BA76_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0209</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95766.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798375.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0209"/>
    <dbReference type="EnsemblBacteria" id="AEF95766">
        <property type="protein sequence ID" value="AEF95766"/>
        <property type="gene ID" value="Metig_0209"/>
    </dbReference>
    <dbReference type="GeneID" id="10643044"/>
    <dbReference type="KEGG" id="mig:Metig_0209"/>
    <dbReference type="HOGENOM" id="CLU_335764_0_0_2"/>
    <dbReference type="OMA" id="AVYIDAY"/>
    <dbReference type="OrthoDB" id="2173at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="129"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF95766.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="858" mass="101521" checksum="EF726D9CD7C6D112" modified="2011-07-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-09-29" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F6BA99</accession>
    <name>F6BA99_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="3">AAA ATPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_0232</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the archaeal ATPase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95789.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798398.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF95789">
        <property type="protein sequence ID" value="AEF95789"/>
        <property type="gene ID" value="Metig_0232"/>
    </dbReference>
    <dbReference type="GeneID" id="10643067"/>
    <dbReference type="KEGG" id="mig:Metig_0232"/>
    <dbReference type="HOGENOM" id="CLU_068608_0_0_2"/>
    <dbReference type="OrthoDB" id="50712at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011579">
        <property type="entry name" value="ATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01637">
        <property type="entry name" value="ATPase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA" evidence="2">
        <location>
            <begin position="21"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF95789.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="359" mass="42332" checksum="F6584952D9FB4B2C" modified="2011-07-27" version="1">MEFFNREKEIKITLSIIEDEPDDTYFIYGPINSGKTTLINEIINNKLNKDKYVVFYINLRRYFISKYDDFIALLFKEYSEDKKPLELIKGLIKDSPLIFGIPIPKNTLEILLSKNEEVNVFDYITNFLLDIKKEGKQPIIILDELQKIGDLKINGFLIYELFNYFISLTKEMHLSHVFCLSSDSLFIERVYNEAVLNGRAKYLLVDDFDKETAMKFMDFLSERILNKKLSNEEKELIYSYVGGKPKDIIYVIKRLRVENLKEILDYLLNDAKQKLDMLLEVLDYSTPKVVVGDKEVEVKKEDIINALRLFKEKYMINKKEIPTPVYMYLVKKNILFLNPVEGTLRPQSFLIWNGIKRLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAD5</accession>
    <name>F6BAD5_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Sirohydrochlorin cobaltochelatase</fullName>
            <ecNumber evidence="4">4.99.1.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">CbiXS</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">Sirohydrochlorin nickelchelatase</fullName>
            <ecNumber evidence="4">4.99.1.11</ecNumber>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">cbiX</name>
        <name evidence="4" type="synonym">cfbA</name>
        <name evidence="6" type="ordered locus">Metig_0269</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Catalyzes the insertion of Co(2+) into sirohydrochlorin as part of the anaerobic pathway to cobalamin biosynthesis. Involved in the biosynthesis of the unique nickel-containing tetrapyrrole coenzyme F430, the prosthetic group of methyl-coenzyme M reductase (MCR), which plays a key role in methanogenesis and anaerobic methane oxidation. Catalyzes the insertion of Ni(2+) into sirohydrochlorin to yield Ni-sirohydrochlorin.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>2 H(+) + Ni-sirohydrochlorin = Ni(2+) + sirohydrochlorin</text>
            <dbReference type="Rhea" id="RHEA:52796"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
            <dbReference type="ChEBI" id="CHEBI:58351"/>
            <dbReference type="ChEBI" id="CHEBI:136841"/>
            <dbReference type="EC" id="4.99.1.11"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>Co-sirohydrochlorin + 2 H(+) = Co(2+) + sirohydrochlorin</text>
            <dbReference type="Rhea" id="RHEA:15893"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
            <dbReference type="ChEBI" id="CHEBI:58351"/>
            <dbReference type="ChEBI" id="CHEBI:60049"/>
            <dbReference type="EC" id="4.99.1.3"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">Cofactor biosynthesis; adenosylcobalamin biosynthesis; cob(II)yrinate a,c-diamide from sirohydrochlorin (anaerobic route): step 1/10.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homotetramer; dimer of dimers.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the CbiX family. CbiXS subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.99.1.3" evidence="4"/>
    <dbReference type="EC" id="4.99.1.11" evidence="4"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95825.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798434.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0269"/>
    <dbReference type="EnsemblBacteria" id="AEF95825">
        <property type="protein sequence ID" value="AEF95825"/>
        <property type="gene ID" value="Metig_0269"/>
    </dbReference>
    <dbReference type="GeneID" id="10643104"/>
    <dbReference type="KEGG" id="mig:Metig_0269"/>
    <dbReference type="HOGENOM" id="CLU_065901_2_1_2"/>
    <dbReference type="OMA" id="HEHEKLE"/>
    <dbReference type="OrthoDB" id="93992at2157"/>
    <dbReference type="UniPathway" id="UPA00148">
        <property type="reaction ID" value="UER00223"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050897">
        <property type="term" value="F:cobalt ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016151">
        <property type="term" value="F:nickel cation binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016852">
        <property type="term" value="F:sirohydrochlorin cobaltochelatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019251">
        <property type="term" value="P:anaerobic cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015948">
        <property type="term" value="P:methanogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00785">
        <property type="entry name" value="CbiX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002762">
        <property type="entry name" value="CbiX-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023652">
        <property type="entry name" value="SiroHydchlorin_Cochelatase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01903">
        <property type="entry name" value="CbiX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4" id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword evidence="4" id="KW-0170">Cobalt</keyword>
    <keyword evidence="3 4" id="KW-0456">Lyase</keyword>
    <keyword evidence="2 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0484">Methanogenesis</keyword>
    <keyword evidence="4" id="KW-0533">Nickel</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Substrate binding" evidence="4">
        <location>
            <begin position="70"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="92"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="4">
        <location>
            <position position="9"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="9"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="45"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEF95825.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="145" mass="16633" checksum="B2D68DF853383372" modified="2011-07-27" version="1">MEALVLVGHGSRLPYSKEVVEKIAEKIRAKNIYPIVEVGMMEFNEPTIPQAVKKAIEQGAKKIIVVPVFLAHGNHTKRDIPRILGLIEDDGEHHHNHKHEHHHHHHHHHEHEKLEIPDDVEIIYREPLGADDRIVDIVLDRAAGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAE1</accession>
    <name>F6BAE1_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Cl-channel voltage-gated family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Metig_0275</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95831.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798440.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0275"/>
    <dbReference type="EnsemblBacteria" id="AEF95831">
        <property type="protein sequence ID" value="AEF95831"/>
        <property type="gene ID" value="Metig_0275"/>
    </dbReference>
    <dbReference type="GeneID" id="10643110"/>
    <dbReference type="KEGG" id="mig:Metig_0275"/>
    <dbReference type="HOGENOM" id="CLU_015263_5_3_2"/>
    <dbReference type="OMA" id="NPYYRVL"/>
    <dbReference type="OrthoDB" id="43898at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005247">
        <property type="term" value="F:voltage-gated chloride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014743">
        <property type="entry name" value="Cl-channel_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001807">
        <property type="entry name" value="Cl-channel_volt-gated"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00654">
        <property type="entry name" value="Voltage_CLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00762">
        <property type="entry name" value="CLCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81340">
        <property type="entry name" value="SSF81340"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="48"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="135"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="172"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="210"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="250"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="277"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="309"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="337"/>
            <end position="359"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEF95831.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="394" mass="42257" checksum="B7F5F22AB80028A8" modified="2011-07-27" version="1">MIVDMSNLKMIIKWLYVSSLIGVVGSLSAIFISIIVHFAYLEGHFNPIYIPFALAFSGLLVDFCYNLKGSGVDRVLKALKNNIPLNPIVGFLKIIAAGIVIGFGGSAGKEGPCVQSSASFADVLYEVLKLKYRKAVIISGIAGGLSGAFCAPLGSAIFASEIVNGHDFSYRSLFPSIFASIIGYFTYYSITGKKHLFNIDLSYSLTPFDFIMFFICAVFCSMTAFLYISTYGKIRDYFDSFKCPQCLKSFIGGLVVALIGYFVPQVLGLGIDVISNLFFVKYGVVLLILMLLGKILATTFTVGVGTPGGLVIPSMFVGAVSGALFGTLIGVENIAPFVILGIAASMASIANVPLASAILCTEIFGFDFAVPAAIGALLGYQLTRWNIIYKYISL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAH6</accession>
    <name>F6BAH6_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Rubrerythrin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_1454</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96989.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799585.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1454"/>
    <dbReference type="EnsemblBacteria" id="AEF96989">
        <property type="protein sequence ID" value="AEF96989"/>
        <property type="gene ID" value="Metig_1454"/>
    </dbReference>
    <dbReference type="GeneID" id="10644327"/>
    <dbReference type="KEGG" id="mig:Metig_1454"/>
    <dbReference type="HOGENOM" id="CLU_095256_0_1_2"/>
    <dbReference type="OMA" id="AMARCRY"/>
    <dbReference type="OrthoDB" id="82596at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01041">
        <property type="entry name" value="Rubrerythrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012347">
        <property type="entry name" value="Ferritin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009040">
        <property type="entry name" value="Ferritin-like_diiron"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009078">
        <property type="entry name" value="Ferritin-like_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024934">
        <property type="entry name" value="Rubredoxin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003251">
        <property type="entry name" value="Rubrerythrin"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02915">
        <property type="entry name" value="Rubrerythrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47240">
        <property type="entry name" value="SSF47240"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50905">
        <property type="entry name" value="FERRITIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50903">
        <property type="entry name" value="RUBREDOXIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ferritin-like diiron" evidence="2">
        <location>
            <begin position="1"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="domain" description="Rubredoxin-like" evidence="1">
        <location>
            <begin position="150"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF96989.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="189" mass="21614" checksum="1A717172D3E57D3A" modified="2011-07-27" version="1">MKTLENLVKAFAGESMARNRYQLYAKIAKEEGYIFVSKVFLETAENEKEHAETLFKLIQKLRRGESIKTEVKTSMVCGDTVENLKAAIDGEHHENTIMYPEFAEIAENEGLNDIAGKLRAIAKAEEHHERRYRKILEAIENGTMFKRDEEVEWVCLECGYIFKGTEPPEKCPSCGHPKGYYVATDMLSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAJ3</accession>
    <name>F6BAJ3_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">SMC domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1472</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97006.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799602.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1472"/>
    <dbReference type="EnsemblBacteria" id="AEF97006">
        <property type="protein sequence ID" value="AEF97006"/>
        <property type="gene ID" value="Metig_1472"/>
    </dbReference>
    <dbReference type="GeneID" id="10644345"/>
    <dbReference type="KEGG" id="mig:Metig_1472"/>
    <dbReference type="HOGENOM" id="CLU_035814_3_1_2"/>
    <dbReference type="OMA" id="ITYHLEI"/>
    <dbReference type="OrthoDB" id="38516at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014555">
        <property type="entry name" value="RecF-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13304">
        <property type="entry name" value="AAA_21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF029347">
        <property type="entry name" value="RecF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATPase_AAA_core" evidence="1">
        <location>
            <begin position="24"/>
            <end position="364"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF97006.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="416" mass="47636" checksum="C81E51BC8A066CBA" modified="2011-07-27" version="1">MVIKRICVKNFKSFKELDVDLGKFNVLIGPNASGKSNFIQIFKFLRDLSMYGLDNAISMQGGIEYLRNMKIGPSENLSIRVVFDKKFNVPILSEKNQKFSMEVCEVIYDFVMEFKKRGLGFKSVNERLTLKCNFIEENTNKKLGEGKLICSLNKGKLRIKLETPSKLPLKKEDIVFPPSLKLLESKLPNNISILKVSFAILPLIPIIDDLQNAAIYDIDPKLSKKATQITGKFELEEDGSNLSIVLKNLIENKEKKRKLYNLISELLPFVDDLNVEKFEDKSLLFKLREKYFKEQYLPASLISDGTINITALIIAVYFEEKPLTIIEEPERNIHPYLISKVIEMMKDASRKKQIIVTTHNPEMVKYAGLDNILVVSRNKEGFSTISRPLDKKEIKVFLENEIGIEELYVKNILGGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAK8</accession>
    <name>F6BAK8_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein-export membrane protein SecF</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">secF</name>
        <name evidence="10" type="ordered locus">Metig_1487</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Involved in protein export.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Part of the protein translocation apparatus. Forms a complex with SecD.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cell membrane</location>
            <topology evidence="9">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the SecD/SecF family. SecF subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97021.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799616.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1487"/>
    <dbReference type="EnsemblBacteria" id="AEF97021">
        <property type="protein sequence ID" value="AEF97021"/>
        <property type="gene ID" value="Metig_1487"/>
    </dbReference>
    <dbReference type="GeneID" id="10644360"/>
    <dbReference type="KEGG" id="mig:Metig_1487"/>
    <dbReference type="HOGENOM" id="CLU_060478_0_0_2"/>
    <dbReference type="OMA" id="FMAIVVY"/>
    <dbReference type="OrthoDB" id="63405at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0065002">
        <property type="term" value="P:intracellular protein transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006605">
        <property type="term" value="P:protein targeting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01464_A">
        <property type="entry name" value="SecF_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022813">
        <property type="entry name" value="SecD/SecF_arch_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024921">
        <property type="entry name" value="SecF_arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30081">
        <property type="entry name" value="PTHR30081"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02355">
        <property type="entry name" value="SecD_SecF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 9" id="KW-0811">Translocation</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="7"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="121"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="147"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="175"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="216"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="247"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AEF97021.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="281" mass="30974" checksum="9478A4232FEF8E23" modified="2011-07-27" version="1">MKINYKILTIVPIILTLLSAIFVAVHGIPKSIDVVGGTEITIKAPKDTNIEQLKEILKNAEVKKLESQSGTFIVIRVDKNTNIDEVRKVLKEFFGVDDLSKLDYGEKQIGPSLSEKFWEEGIKAVGFAFLFMAAVVYFVFRNPIPSGAVILAALSDIILALGGMSLFSIPLSTATIAALLMLIGYSVDTDIMLTTRVLKRKSDGLDERIKDAMKTGITMSVTTIVAMMVLYLVVTFFVPVAETLKNIAAVLLIGLIADLMTTWLTNAGILRYYISEYRKEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAM1</accession>
    <name>F6BAM1_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Phosphate-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Metig_0279</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the PstS family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95835.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798444.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0279"/>
    <dbReference type="EnsemblBacteria" id="AEF95835">
        <property type="protein sequence ID" value="AEF95835"/>
        <property type="gene ID" value="Metig_0279"/>
    </dbReference>
    <dbReference type="GeneID" id="10643114"/>
    <dbReference type="KEGG" id="mig:Metig_0279"/>
    <dbReference type="HOGENOM" id="CLU_034528_1_2_2"/>
    <dbReference type="OMA" id="FPYPVYA"/>
    <dbReference type="OrthoDB" id="40323at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042301">
        <property type="term" value="F:phosphate ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035435">
        <property type="term" value="P:phosphate ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005673">
        <property type="entry name" value="ABC_phos-bd_PstS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024370">
        <property type="entry name" value="PBP_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12849">
        <property type="entry name" value="PBP_like_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002756">
        <property type="entry name" value="PstS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00975">
        <property type="entry name" value="3a0107s03"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0592">Phosphate transport</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="PBP_domain" evidence="5">
        <location>
            <begin position="47"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="29"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRNR" id="PIRNR002756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF12849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEF95835.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="390" mass="42700" checksum="58772A8CF6526063" modified="2011-07-27" version="1">MKKLLSILVGALLVVSIVSLCGCVNNQQTTTEKSTNNQQTTTEKPTNNQQTTKTIIIRTTGATFPKAQIEKWIRDYQKVNPNVKIEYEGGGSGHGQDAFLKGLTDIGRTDPPVKKEMWEKFLSTGDQPLQFPEIVGAVVVTYNVPEIGNAQLKLTPKVIADIYQGKIEYWDDEAIKKLNPGLADKLPHEKIIVIYRSDASGTTAIFSTFLNIAGGLPDNLVGKSVQWPVEKLGRGIGAKGNPGVINALKSTKYSIAYTELSYTIENNLPNAAIMNKKGKFVAPTDESIKAAVSGVKANIPDPTEGYKEDLKQMLNSPNENAYPIVAFTHLLVWENKNGKHYSPEKAKAIKEFLKWVLTEGQKPEHIAPGYVGLPSDVAKIGLDAINKIKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAP7</accession>
    <name>F6BAP7_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Thiamine-monophosphate kinase</fullName>
            <shortName evidence="1">TMP kinase</shortName>
            <shortName evidence="1">Thiamine-phosphate kinase</shortName>
            <ecNumber evidence="1">2.7.4.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">thiL</name>
        <name evidence="3" type="ordered locus">Metig_0305</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the ATP-dependent phosphorylation of thiamine-monophosphate (TMP) to form thiamine-pyrophosphate (TPP), the active form of vitamin B1.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + thiamine phosphate = ADP + thiamine diphosphate</text>
            <dbReference type="Rhea" id="RHEA:15913"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:37575"/>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.16"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; thiamine diphosphate biosynthesis; thiamine diphosphate from thiamine phosphate: step 1/1.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="1">Reaction mechanism of ThiL seems to utilize a direct, inline transfer of the gamma-phosphate of ATP to TMP rather than a phosphorylated enzyme intermediate.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the thiamine-monophosphate kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="1">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.16" evidence="1"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95861.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798470.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0305"/>
    <dbReference type="EnsemblBacteria" id="AEF95861">
        <property type="protein sequence ID" value="AEF95861"/>
        <property type="gene ID" value="Metig_0305"/>
    </dbReference>
    <dbReference type="GeneID" id="10643140"/>
    <dbReference type="KEGG" id="mig:Metig_0305"/>
    <dbReference type="HOGENOM" id="CLU_046964_2_0_2"/>
    <dbReference type="OMA" id="HFRRDWS"/>
    <dbReference type="OrthoDB" id="104636at2157"/>
    <dbReference type="UniPathway" id="UPA00060">
        <property type="reaction ID" value="UER00142"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009030">
        <property type="term" value="F:thiamine-phosphate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009228">
        <property type="term" value="P:thiamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009229">
        <property type="term" value="P:thiamine diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02194">
        <property type="entry name" value="ThiL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.650.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02128">
        <property type="entry name" value="TMP_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036676">
        <property type="entry name" value="PurM-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016188">
        <property type="entry name" value="PurM-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036921">
        <property type="entry name" value="PurM-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006283">
        <property type="entry name" value="ThiL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30270">
        <property type="entry name" value="PTHR30270"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00586">
        <property type="entry name" value="AIRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005303">
        <property type="entry name" value="Thiam_monoph_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55326">
        <property type="entry name" value="SSF55326"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56042">
        <property type="entry name" value="SSF56042"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01379">
        <property type="entry name" value="thiL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0418">Kinase</keyword>
    <keyword evidence="1" id="KW-0460">Magnesium</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0784">Thiamine biosynthesis</keyword>
    <keyword evidence="1 3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="AIRS" evidence="2">
        <location>
            <begin position="26"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="27"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="41"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>4</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="42"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="43"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="50"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="71"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="119"/>
            <end position="120"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="120"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="143"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="216"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="218"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="219"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>5</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="266"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="314"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF95861.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="318" mass="35754" checksum="8FF53270B03869CA" modified="2011-07-27" version="1">MNELEIINLISKNLTYKGNAIKSIGDDCAVFKINNTYLVITTDMMFKSTHFPDILTPFQIGGRLITANVSDIAAMCAKPLGIVVSIGISKEYANKKFIDELSKGLNFMAEKYKCPVVGGDTNKSNELTLCGTAFGLTDNPIYRRAEVGDDICITNDLGRVFCALKMIEMRDNGIINKNQFNKLMEKYPNIMKKLCEPEARVEEGILMNELVNSCCDISDGLAKDLFYIGGFEIYSEDLLKAVPEDVLEFCEEFNINPIEAVLNSGEEFELLFTTKKYLKVKEILKKTDTKVIKIGKVIEDGKYLDGNIFKFGGYIHRW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAP9</accession>
    <name>F6BAP9_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0307</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95863.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798472.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF95863">
        <property type="protein sequence ID" value="AEF95863"/>
        <property type="gene ID" value="Metig_0307"/>
    </dbReference>
    <dbReference type="GeneID" id="10643142"/>
    <dbReference type="KEGG" id="mig:Metig_0307"/>
    <dbReference type="HOGENOM" id="CLU_2177897_0_0_2"/>
    <dbReference type="OMA" id="MRLMPHF"/>
    <dbReference type="OrthoDB" id="106599at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035350">
        <property type="entry name" value="DUF5400"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17379">
        <property type="entry name" value="DUF5400"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="31"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="57"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="86"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF95863.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="109" mass="11960" checksum="0424E7D3B3AAB4BB" modified="2011-07-27" version="1">MNEVIMLVSLSVIFGAMLSGFATFRMTGMRLMPHFASLIIAFILTLASLFVDNNIVFYSAIAFQIIAPITICGTICNILKTQFQNTGIYSSHLALMGMLFILAIGNLFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2020-08-12" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAQ6</accession>
    <name>F6BAQ6_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_0314</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95870.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF95870">
        <property type="protein sequence ID" value="AEF95870"/>
        <property type="gene ID" value="Metig_0314"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0314"/>
    <dbReference type="HOGENOM" id="CLU_200897_0_0_2"/>
    <dbReference type="OMA" id="EVDVCPC"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF95870.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="54" mass="6310" checksum="D75C28EF437C9485" modified="2011-07-27" version="1">MIVWNLICPNCKRRVTLKLDVCPCMASQIPIPKCENCNTEMTFDINAKRGRRRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAT8</accession>
    <name>F6BAT8_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Methanogenesis marker protein 6</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_0346</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95902.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798511.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0346"/>
    <dbReference type="EnsemblBacteria" id="AEF95902">
        <property type="protein sequence ID" value="AEF95902"/>
        <property type="gene ID" value="Metig_0346"/>
    </dbReference>
    <dbReference type="GeneID" id="10643183"/>
    <dbReference type="KEGG" id="mig:Metig_0346"/>
    <dbReference type="HOGENOM" id="CLU_132457_1_0_2"/>
    <dbReference type="OMA" id="ETCFGAY"/>
    <dbReference type="OrthoDB" id="106827at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012025">
        <property type="entry name" value="UCP005642_methan"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09875">
        <property type="entry name" value="DUF2102"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005642">
        <property type="entry name" value="UCP005642"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03272">
        <property type="entry name" value="methan_mark_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF95902.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="150" mass="17500" checksum="019500592B308E11" modified="2011-07-27" version="1">MKTKVIVLADNAETTPSKLFRFLNSLNYDINVKETCFGAYIEGEDDVVDKVANIVRNLEKNRIFCKDRGFPIWDKRRCRAFRGGGPREGFHQLEAEQKILDKIAKALDEIEKEGIHQIDEIEKEKVDDKTKKLDVETFKKIIDEEFKHNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAV0</accession>
    <name>F6BAV0_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Small GTP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metig_1503</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97037.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799631.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1503"/>
    <dbReference type="EnsemblBacteria" id="AEF97037">
        <property type="protein sequence ID" value="AEF97037"/>
        <property type="gene ID" value="Metig_1503"/>
    </dbReference>
    <dbReference type="GeneID" id="10644376"/>
    <dbReference type="KEGG" id="mig:Metig_1503"/>
    <dbReference type="HOGENOM" id="CLU_011784_0_0_2"/>
    <dbReference type="OMA" id="DPSEYCG"/>
    <dbReference type="OrthoDB" id="66209at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031167">
        <property type="entry name" value="G_OBG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041623">
        <property type="entry name" value="NOG1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010674">
        <property type="entry name" value="NOG1_Rossman_fold_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06858">
        <property type="entry name" value="NOG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17835">
        <property type="entry name" value="NOG1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51710">
        <property type="entry name" value="G_OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="OBG-type G" evidence="3">
        <location>
            <begin position="175"/>
            <end position="336"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEF97037.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="339" mass="38305" checksum="A59AE37BDBA8AE5B" modified="2011-07-27" version="1">MRKKGDANPFKKMPTILTPEELMDKAYRRGEKVASELRTTLIGVPKLLKSRTVEEHKIRTMASVVSDNLLKIISKTPSIDRLKPFYKELVEVMVGIDEFKKSLGAVKWASDLVKKLGNEYAKKARRAKTPKEASGFRREFVGRTASILKQIYPNLAFLAVAREKLKDLPTVKELPTLVIAGYPNVGKSTLLRKLTGAEPEINSYPFTTKGLNVGYIGEDIQVVDTPGVLDRPLYERNDIELQAVLALNYLANMILFVLDPSEYCGYSIEEQLNLLKEVSDLFKVPIIVAINKIDLVDGERLKEIEEKLKDYRVIKISADKDIGLDELKKIIVDELLRTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAV2</accession>
    <name>F6BAV2_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Regulator_TrmB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1505</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97039.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799633.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1505"/>
    <dbReference type="EnsemblBacteria" id="AEF97039">
        <property type="protein sequence ID" value="AEF97039"/>
        <property type="gene ID" value="Metig_1505"/>
    </dbReference>
    <dbReference type="GeneID" id="10644378"/>
    <dbReference type="KEGG" id="mig:Metig_1505"/>
    <dbReference type="HOGENOM" id="CLU_1105215_0_0_2"/>
    <dbReference type="OMA" id="EMYKCAW"/>
    <dbReference type="OrthoDB" id="93787at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021586">
        <property type="entry name" value="Tscrpt_reg_TrmB_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11495">
        <property type="entry name" value="Regulator_TrmB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Regulator_TrmB" evidence="1">
        <location>
            <begin position="40"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF11495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF97039.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="250" mass="28268" checksum="57ADF7C58C649136" modified="2011-07-27" version="1">MKKITLLEILVVVAILTTSFAVGYKFLKSNNNTEFSGDEMYKCAWIADKIIKKGFPLYAEIEGRWTSSNEEFKDKVLITKANGGTLFAIYKNQTITIGGKLASKEDIAANKIKLMPLGNTIIKYELAPINAHSFKDIKTKIDIPEHLTVLDVYVYGTFGIDSKTYSPSEQQKIKNYIQYSIPNANLYFTFGGVIFNGKVNLDYLDDLDNLLKPENITTSNLDVYIIVNETISQINMSKLKFNDVRLITWS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAV6</accession>
    <name>F6BAV6_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF1616 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_1509</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97043.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799637.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1509"/>
    <dbReference type="EnsemblBacteria" id="AEF97043">
        <property type="protein sequence ID" value="AEF97043"/>
        <property type="gene ID" value="Metig_1509"/>
    </dbReference>
    <dbReference type="GeneID" id="10644382"/>
    <dbReference type="KEGG" id="mig:Metig_1509"/>
    <dbReference type="HOGENOM" id="CLU_1485907_0_0_2"/>
    <dbReference type="OMA" id="TYYGETW"/>
    <dbReference type="OrthoDB" id="107273at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011674">
        <property type="entry name" value="DUF1616"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07760">
        <property type="entry name" value="DUF1616"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="9"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="domain" description="DUF1616" evidence="2">
        <location>
            <begin position="4"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF97043.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="170" mass="20346" checksum="AECC23862C3F3295" modified="2011-07-27" version="1">MNMKLEKILTYFLLFLLILCIIGTIYLIYAPKIGERFTEFYILNKDLKAYDYPTDLLINESAMVFIGVSNHEYKDMNYTILAFLSDKVYDYNYTVNITILNKWNETLSYKYALSKKVYLRNNETILIPLNFSISIPGRHKIEFILLKDDERKVYRELHLWVNVKEKEELI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAW2</accession>
    <name>F6BAW2_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1515</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97049.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799643.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1515"/>
    <dbReference type="EnsemblBacteria" id="AEF97049">
        <property type="protein sequence ID" value="AEF97049"/>
        <property type="gene ID" value="Metig_1515"/>
    </dbReference>
    <dbReference type="GeneID" id="10644388"/>
    <dbReference type="KEGG" id="mig:Metig_1515"/>
    <dbReference type="HOGENOM" id="CLU_069303_0_0_2"/>
    <dbReference type="OMA" id="YIDEAHH"/>
    <dbReference type="OrthoDB" id="57955at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="254"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF97049.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="324" mass="37277" checksum="074A0A621F7F9B2C" modified="2011-07-27" version="1">MKIEHYILLLIILIGFLTLPLSIPLIKTKQPFSVFNTENNGCSKFFKELYKNNKVKPILFPYKNLKDNSILFIISPDVDFTKEEGEVIKNYIRRGNILVLVDNFNKGNSLLQYLNLSNRFSNKPLYDIVSPYVVINTSYYKGYILIENPTSISGSGTVILYSSLASKVGDYPKPGDEGKYPLILEKNYGNGKIVLISDPDIFKNSLFKHNKGFLLSYFNYSNKIIYVDEIHHSDVNPQNLMTITISRNISPNSLIFLGFVFLLIFIIVENLEFIIKKVLSNPIIKKISHSVGKKEEYSISYFIKKYNVNERIVKEIINKIKVEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAX6</accession>
    <name>F6BAX6_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Methyltransferase type 11</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_1529</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97063.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799657.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF97063">
        <property type="protein sequence ID" value="AEF97063"/>
        <property type="gene ID" value="Metig_1529"/>
    </dbReference>
    <dbReference type="GeneID" id="10644402"/>
    <dbReference type="KEGG" id="mig:Metig_1529"/>
    <dbReference type="HOGENOM" id="CLU_088541_1_0_2"/>
    <dbReference type="OMA" id="QTHLRFF"/>
    <dbReference type="OrthoDB" id="120554at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF97063.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="218" mass="25182" checksum="2344B784A57C6355" modified="2011-07-27" version="1">MLKYATLSTNPYSSHMRIVQLVGNNKKVLDVGCSAGHLSKVFKENGCEVVGIELDDESAKIARNYCIDVIKGDVETINLNYPDEYFDVIVFGDILEHLKNPQTVLIKLRRYLKKDGYIVCSIPNIAHITVRLMLLMGKWEYEDVGLLDRTHLRFFTKKTAIKLAENAGFKIEEVYPTPWIPFFRLRKLHPIFLKIEYTITKMLPTLFALQFVIKARKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAY3</accession>
    <name>F6BAY3_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Phosphonate-transporting ATPase</fullName>
            <ecNumber evidence="5">3.6.3.28</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Metig_1536</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.6.3.28" evidence="5"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97070.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799664.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1536"/>
    <dbReference type="EnsemblBacteria" id="AEF97070">
        <property type="protein sequence ID" value="AEF97070"/>
        <property type="gene ID" value="Metig_1536"/>
    </dbReference>
    <dbReference type="GeneID" id="10644409"/>
    <dbReference type="KEGG" id="mig:Metig_1536"/>
    <dbReference type="HOGENOM" id="CLU_000604_1_11_2"/>
    <dbReference type="OMA" id="LPMYQRA"/>
    <dbReference type="OrthoDB" id="68573at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="3"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="35"/>
            <end position="42"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEF97070.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="251" mass="28329" checksum="ECFD0E56762B73E0" modified="2011-07-27" version="1">MILSVDGVEFSYKSRKILENVKFKVNRGEVVSILGPNGCGKSTLLKCINKILKPKRGVILIDNYNLNELSNLEIAKKIGYVPQKSDGSYMTVFDTILLGRKPHIKWEVSKQDINLVNEILEIMDLKDYALRYTNELSGGELQKVIIARAIVQEPEVLLLDEPTNNLDLKNQLEILRVIRNISKSKNIASIIVIHDLNLALRYSDKFIMLKDGKIFAEGGKEVINPKNIEAVYGVSVDVKYYNGIPVVIPLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>F6BB16</accession>
    <name>F6BB16_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="6">ABC transporter related protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Metig_1569</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97103.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799697.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1569"/>
    <dbReference type="EnsemblBacteria" id="AEF97103">
        <property type="protein sequence ID" value="AEF97103"/>
        <property type="gene ID" value="Metig_1569"/>
    </dbReference>
    <dbReference type="GeneID" id="10644442"/>
    <dbReference type="KEGG" id="mig:Metig_1569"/>
    <dbReference type="HOGENOM" id="CLU_017344_4_1_2"/>
    <dbReference type="OMA" id="ISYKPQY"/>
    <dbReference type="OrthoDB" id="13010at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013283">
        <property type="entry name" value="RLI1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007209">
        <property type="entry name" value="RNaseL-inhib-like_metal-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19248">
        <property type="entry name" value="PTHR19248"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00037">
        <property type="entry name" value="Fer4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04068">
        <property type="entry name" value="RLI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01868">
        <property type="entry name" value="ABCEFAMILY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="5">
        <location>
            <begin position="42"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="66"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="310"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="106"/>
            <end position="113"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="370"/>
            <end position="377"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEF97103.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="588" mass="66334" checksum="2F8A2C827959A2D2" modified="2011-07-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>F6BB27</accession>
    <name>F6BB27_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Archaeoflavoprotein AfpA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0356</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95912.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798521.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0356"/>
    <dbReference type="EnsemblBacteria" id="AEF95912">
        <property type="protein sequence ID" value="AEF95912"/>
        <property type="gene ID" value="Metig_0356"/>
    </dbReference>
    <dbReference type="GeneID" id="10643193"/>
    <dbReference type="KEGG" id="mig:Metig_0356"/>
    <dbReference type="HOGENOM" id="CLU_098523_0_0_2"/>
    <dbReference type="OMA" id="IYPPDNK"/>
    <dbReference type="OrthoDB" id="72619at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1950">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014072">
        <property type="entry name" value="Archaeoflavo_AfpA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036551">
        <property type="entry name" value="Flavin_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003382">
        <property type="entry name" value="Flavoprotein"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02441">
        <property type="entry name" value="Flavoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52507">
        <property type="entry name" value="SSF52507"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02699">
        <property type="entry name" value="archaeo_AfpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Flavoprotein" evidence="1">
        <location>
            <begin position="3"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF95912.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="190" mass="21740" checksum="085AF0CCFB6B090B" modified="2011-07-27" version="1">MLKIAWGITGCGDKLPEVVEIMKKLKNKYNLDVDIYLSKNAKIVVKWYKLWEVLEDEFYDLRVEVNSNAPFLVGKLQTGKYDLFLVAPATANTTAKIAYGIADTLITNSVAQAMKARVPVYIFPPDNKKGKIETLLPGNKKLTLYIRDVDVENVEKIRRMEGIEVLDNPEDIEKVILKHIEMKKQKENKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F6BB69</accession>
    <name>F6BB69_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Radical SAM domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Metig_0398</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95954.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0398"/>
    <dbReference type="EnsemblBacteria" id="AEF95954">
        <property type="protein sequence ID" value="AEF95954"/>
        <property type="gene ID" value="Metig_0398"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0398"/>
    <dbReference type="HOGENOM" id="CLU_053467_0_0_2"/>
    <dbReference type="OMA" id="DCFYCPL"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaB/NifB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040087">
        <property type="entry name" value="MJ0021-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43288:SF1">
        <property type="entry name" value="PTHR43288:SF1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01108">
        <property type="entry name" value="Uncharacterised_Radical_SAM_Su"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="5">
        <location>
            <begin position="12"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEF95954.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="399" mass="46013" checksum="2010AAFB0E074BCB" modified="2011-07-27" version="1">MVVMELPKGCQYCIRGEKLVLFVTGLCNQNCYYCPLSEKRKGKDVIYANERQITSIEEAIEEAYLCSSKGVGITGGNPLLRINRTCEYLKALKNEFGEKFHAHLYTTPDLIDREKLEKLQDAGLDEIRLHPTKIFNEIGAKQKAASTATEGCREVEYEYSTKSEGFCSNADEKYYNLLLDKLKLCMEYIEDVGVEIPSIPNMEKEILNLAYKLDEIGVKFLNINELEFSETTYETLIAKGFEVKDDISSAIKGSEETALYVSENFKGNMIINYCPSSLKDNIQLRNRLINRAKNVAKDYEVITDEGLLLRGIMIFDNEEDLEEIAEILRDNEVEFEIVENKIYLNPFVLEDLIEEMKRARYEIKFSAYISEVYPTADALEVERIPLVTKKLRFRRRRKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>F6BB75</accession>
    <name>F6BB75_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Xenobiotic-transporting ATPase</fullName>
            <ecNumber evidence="5">3.6.3.44</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Metig_0404</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.6.3.44" evidence="5"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95960.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798569.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0404"/>
    <dbReference type="EnsemblBacteria" id="AEF95960">
        <property type="protein sequence ID" value="AEF95960"/>
        <property type="gene ID" value="Metig_0404"/>
    </dbReference>
    <dbReference type="GeneID" id="10643241"/>
    <dbReference type="KEGG" id="mig:Metig_0404"/>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_2"/>
    <dbReference type="OMA" id="ILITHHE"/>
    <dbReference type="OrthoDB" id="44302at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03217">
        <property type="entry name" value="ABC_FeS_Assembly"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010230">
        <property type="entry name" value="FeS-cluster_ATPase_SufC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43204">
        <property type="entry name" value="PTHR43204"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="3"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="35"/>
            <end position="42"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEF95960.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="248" mass="27842" checksum="E3BAAFD9D5F148FC" modified="2011-07-27" version="1">MLLVVKDLYLKRGDRMILKGLNLEIEENEIHAVVGPNGAGKSTLSYTLMGCSGYEPVKGEIIFKGINIVDKSITERARMGMTLAWQEPARFEGITVKQYLSLGMKEYDEEKIYKALEFVNLCPDTYLNRFVDDSLSGGERKRIELASILCMEPDLAILDEPDSGIDIVSLDEITKLFKHLKKNGSSVLVITHREEITNCADKASLLCDGRVIKTGDPKEVGEYYKRECGKCLVKVPSIKKEVEEDNNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F6BB76</accession>
    <name>F6BB76_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">SufBD protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_0405</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95961.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798570.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0405"/>
    <dbReference type="EnsemblBacteria" id="AEF95961">
        <property type="protein sequence ID" value="AEF95961"/>
        <property type="gene ID" value="Metig_0405"/>
    </dbReference>
    <dbReference type="GeneID" id="10643242"/>
    <dbReference type="KEGG" id="mig:Metig_0405"/>
    <dbReference type="HOGENOM" id="CLU_894122_0_0_2"/>
    <dbReference type="OMA" id="PVHLCFG"/>
    <dbReference type="OrthoDB" id="35631at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000825">
        <property type="entry name" value="SUF_FeS_clus_asmbl_SufBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037284">
        <property type="entry name" value="SUF_FeS_clus_asmbl_SufBD_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01458">
        <property type="entry name" value="SUFBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101960">
        <property type="entry name" value="SSF101960"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF95961.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="313" mass="34308" checksum="AC5C5FF688D94C94" modified="2011-07-27" version="1">MDKDLKEILEAYKLTNGNPEELIHGKGPRMIVKENKILGVKDAEGIILDGKVVDGKIVAKIIVKEGYKFDTPMHMCFGVTEEEAEQIVDVEIILEDNSEITLMSHCSFPRAKNVKHIMDGKITVGKNAKFVYKEVHFHGEEGGVLVKPTVKATVKEGGVYISELTLTKGRIGFLEINADIDVEKNGVVDITTKTYAVKDDKVIINETVKLNEEGGKCIIKSRGAAKDNSEVVLKLKIEGNAPYCKGHIDCAEVIKGDAIVESIPIVVVRDDRARITHEAAIGSVDKKQVETLMAKGLDEDEATEIIVKGMIGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>F6BB91</accession>
    <name>F6BB91_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative monovalent cation/H+ antiporter subunit G</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0420</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95976.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798585.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0420"/>
    <dbReference type="EnsemblBacteria" id="AEF95976">
        <property type="protein sequence ID" value="AEF95976"/>
        <property type="gene ID" value="Metig_0420"/>
    </dbReference>
    <dbReference type="GeneID" id="10643257"/>
    <dbReference type="KEGG" id="mig:Metig_0420"/>
    <dbReference type="HOGENOM" id="CLU_171540_0_0_2"/>
    <dbReference type="OMA" id="GVIDCAC"/>
    <dbReference type="OrthoDB" id="120304at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="35"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="58"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF95976.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="84" mass="9460" checksum="33794C44FCFE2E9A" modified="2011-07-27" version="1">MLDNVVLIVASAGILLASIRLWNEEDRKNILYARLHIAGVIDIACIIIMLMMNQPLLALVYLILCPFAAHAIANANYYDKYNKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>F6BB95</accession>
    <name>F6BB95_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Carboxymuconolactone decarboxylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0424</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95980.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798589.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0424"/>
    <dbReference type="EnsemblBacteria" id="AEF95980">
        <property type="protein sequence ID" value="AEF95980"/>
        <property type="gene ID" value="Metig_0424"/>
    </dbReference>
    <dbReference type="GeneID" id="10643261"/>
    <dbReference type="KEGG" id="mig:Metig_0424"/>
    <dbReference type="HOGENOM" id="CLU_137228_5_0_2"/>
    <dbReference type="OMA" id="VASKCDE"/>
    <dbReference type="OrthoDB" id="108307at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051920">
        <property type="term" value="F:peroxiredoxin activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1290.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029032">
        <property type="entry name" value="AhpD-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003779">
        <property type="entry name" value="CMD-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02627">
        <property type="entry name" value="CMD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69118">
        <property type="entry name" value="SSF69118"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CMD" evidence="1">
        <location>
            <begin position="19"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02627"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF95980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="104" mass="11633" checksum="691AB1CC85845506" modified="2011-07-27" version="1">MKNEVFFGEGMKTVKEKYPDLYEAIVKLNEAAFTGKVLDYKTQKLIAIGIVASKCDEVATERQMRSGMKELGITKEEIADVLRVVLLTSGMPAFTKAMKILEKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBA0</accession>
    <name>F6BBA0_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Ferroxidase</fullName>
            <ecNumber evidence="2">1.16.3.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0429</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.16.3.1" evidence="2"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95985.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798594.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0429"/>
    <dbReference type="EnsemblBacteria" id="AEF95985">
        <property type="protein sequence ID" value="AEF95985"/>
        <property type="gene ID" value="Metig_0429"/>
    </dbReference>
    <dbReference type="GeneID" id="10643266"/>
    <dbReference type="KEGG" id="mig:Metig_0429"/>
    <dbReference type="HOGENOM" id="CLU_065681_1_2_2"/>
    <dbReference type="OMA" id="CEDKGFE"/>
    <dbReference type="OrthoDB" id="87619at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008199">
        <property type="term" value="F:ferric iron binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004322">
        <property type="term" value="F:ferroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006879">
        <property type="term" value="P:cellular iron ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006826">
        <property type="term" value="P:iron ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01055">
        <property type="entry name" value="Nonheme_Ferritin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001519">
        <property type="entry name" value="Ferritin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012347">
        <property type="entry name" value="Ferritin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009040">
        <property type="entry name" value="Ferritin-like_diiron"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009078">
        <property type="entry name" value="Ferritin-like_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008331">
        <property type="entry name" value="Ferritin_DPS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041719">
        <property type="entry name" value="Ferritin_prok"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11431">
        <property type="entry name" value="PTHR11431"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00210">
        <property type="entry name" value="Ferritin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47240">
        <property type="entry name" value="SSF47240"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50905">
        <property type="entry name" value="FERRITIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ferritin-like diiron" evidence="1">
        <location>
            <begin position="1"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF95985.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="165" mass="19207" checksum="3C1DD507AFFB6F53" modified="2011-07-27" version="1">MIKENILDALNKQINREFYSAYLYLSMSAYAESIGLKGFAQWLKVQSQEELDHAMKIYDYVIERGGKVKLYSIEEPKSEWSSIIEVFEDGYKHEVSITQSINDIMDLAMSEKDYATVNMLQWFVTEQVEEEASFSEILDKLKLIGDDKRGLFMLDKELGQRTYSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBA3</accession>
    <name>F6BBA3_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Rubredoxin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Metig_0432</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Rubredoxin is a small nonheme, iron protein lacking acid-labile sulfide. Its single Fe, chelated to 4 Cys, functions as an electron acceptor and may also stabilize the conformation of the molecule.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Fe(3+)</name>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the rubredoxin family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95988.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798597.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0432"/>
    <dbReference type="EnsemblBacteria" id="AEF95988">
        <property type="protein sequence ID" value="AEF95988"/>
        <property type="gene ID" value="Metig_0432"/>
    </dbReference>
    <dbReference type="GeneID" id="10643269"/>
    <dbReference type="KEGG" id="mig:Metig_0432"/>
    <dbReference type="HOGENOM" id="CLU_128747_1_0_2"/>
    <dbReference type="OrthoDB" id="126197at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00730">
        <property type="entry name" value="rubredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024934">
        <property type="entry name" value="Rubredoxin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024935">
        <property type="entry name" value="Rubredoxin_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00301">
        <property type="entry name" value="Rubredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00163">
        <property type="entry name" value="RUBREDOXIN"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50903">
        <property type="entry name" value="RUBREDOXIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0249">Electron transport</keyword>
    <keyword evidence="5 6" id="KW-0408">Iron</keyword>
    <keyword evidence="3 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Rubredoxin-like" evidence="7">
        <location>
            <begin position="19"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEF95988.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="79" mass="9313" checksum="3A6DE68B16EF9382" modified="2011-07-27" version="1">MVEIKLSCKLDNSCEKPRYRKYRCRVCGWTYDPLKGDPSQNIPPKTPFEELPDTWVCPICRGKIGKESFEPLDEWVEFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBG2</accession>
    <name>F6BBG2_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Methionine--tRNA ligase</fullName>
            <ecNumber evidence="14">6.1.1.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Methionyl-tRNA synthetase</fullName>
            <shortName evidence="14">MetRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">metG</name>
        <name evidence="19" type="ordered locus">Metig_1636</name>
    </gene>
    <organism evidence="20">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 14">
            <text>ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met)</text>
            <dbReference type="Rhea" id="RHEA:13481"/>
            <dbReference type="Rhea" id="RHEA-COMP:9667"/>
            <dbReference type="Rhea" id="RHEA-COMP:9698"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78530"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.10"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="14">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="14">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 14">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 14">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.10" evidence="14"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97169.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799761.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1636"/>
    <dbReference type="EnsemblBacteria" id="AEF97169">
        <property type="protein sequence ID" value="AEF97169"/>
        <property type="gene ID" value="Metig_1636"/>
    </dbReference>
    <dbReference type="GeneID" id="10644509"/>
    <dbReference type="KEGG" id="mig:Metig_1636"/>
    <dbReference type="HOGENOM" id="CLU_009710_1_2_2"/>
    <dbReference type="OMA" id="SDMHGTP"/>
    <dbReference type="OrthoDB" id="6590at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004825">
        <property type="term" value="F:methionine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006431">
        <property type="term" value="P:methionyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07957">
        <property type="entry name" value="Anticodon_Ia_Met"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00814">
        <property type="entry name" value="MetRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02800">
        <property type="entry name" value="tRNA_bind_EcMetRS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.28.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00098">
        <property type="entry name" value="Met_tRNA_synth_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041872">
        <property type="entry name" value="Anticodon_Met"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013155">
        <property type="entry name" value="M/V/L/I-tRNA-synth_anticd-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004495">
        <property type="entry name" value="Met-tRNA-synth_bsu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023458">
        <property type="entry name" value="Met-tRNA_ligase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014758">
        <property type="entry name" value="Met-tRNA_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015413">
        <property type="entry name" value="Methionyl/Leucyl_tRNA_Synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033911">
        <property type="entry name" value="MetRS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029038">
        <property type="entry name" value="MetRS_Zn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002547">
        <property type="entry name" value="tRNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45765">
        <property type="entry name" value="PTHR45765"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08264">
        <property type="entry name" value="Anticodon_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09334">
        <property type="entry name" value="tRNA-synt_1g"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01588">
        <property type="entry name" value="tRNA_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01041">
        <property type="entry name" value="TRNASYNTHMET"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="SSF47323"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57770">
        <property type="entry name" value="SSF57770"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00398">
        <property type="entry name" value="metG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00399">
        <property type="entry name" value="metG_C_term"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50886">
        <property type="entry name" value="TRBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13 14 16" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="10 14 16" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="17" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 14" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 14 16" id="KW-0436">Ligase</keyword>
    <keyword evidence="7 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 14 16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12 14 16" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 14 15" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5 14 15" id="KW-0820">tRNA-binding</keyword>
    <keyword evidence="9 14" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="TRNA-binding" evidence="18">
        <location>
            <begin position="557"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="17">
        <location>
            <begin position="517"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'HIGH' region" evidence="14">
        <location>
            <begin position="10"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'KMSKS' region" evidence="14">
        <location>
            <begin position="322"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="142"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="145"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="154"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="157"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="325"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU363039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="AEF97169.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="659" mass="76434" checksum="F2C90E1E4FBFD84F" modified="2011-07-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-04-07" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBG3</accession>
    <name>F6BBG3_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_1637</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97170.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799762.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF97170">
        <property type="protein sequence ID" value="AEF97170"/>
        <property type="gene ID" value="Metig_1637"/>
    </dbReference>
    <dbReference type="GeneID" id="10644510"/>
    <dbReference type="KEGG" id="mig:Metig_1637"/>
    <dbReference type="HOGENOM" id="CLU_1763880_0_0_2"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001646">
        <property type="entry name" value="5peptide_repeat"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13576">
        <property type="entry name" value="Pentapeptide_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF97170.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="147" mass="17717" checksum="246EF6999AF4E171" modified="2011-07-27" version="1">MAICKRCQRILDRYKNYTPKEVIKDSYCIFHHPSYWKEYGDKVREAFYNEVVDAVTNDKELICVGYYLPEIDFRDFADRIGKDKIIFKKRVIFEEANFQKINFGKVEFRENVYFSKAKFNGIVRFLEVVFDGKVYFSRTAFMGRRSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBJ9</accession>
    <name>F6BBJ9_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">ATP_bind_3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_0452</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96008.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798617.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0452"/>
    <dbReference type="EnsemblBacteria" id="AEF96008">
        <property type="protein sequence ID" value="AEF96008"/>
        <property type="gene ID" value="Metig_0452"/>
    </dbReference>
    <dbReference type="GeneID" id="10643289"/>
    <dbReference type="KEGG" id="mig:Metig_0452"/>
    <dbReference type="HOGENOM" id="CLU_026481_1_1_2"/>
    <dbReference type="OMA" id="MNLDQKQ"/>
    <dbReference type="OrthoDB" id="57500at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034227">
        <property type="term" value="P:tRNA thio-modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002098">
        <property type="term" value="P:tRNA wobble uridine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000541">
        <property type="entry name" value="Ncs6/Tuc1/Ctu1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011063">
        <property type="entry name" value="TilS/TtcA_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035107">
        <property type="entry name" value="tRNA_thiolation_TtcA_Ctu1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020554">
        <property type="entry name" value="UPF0021_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01171">
        <property type="entry name" value="ATP_bind_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004976">
        <property type="entry name" value="ATPase_YdaO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00269">
        <property type="entry name" value="TIGR00269"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01263">
        <property type="entry name" value="UPF0021"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="ATP_bind_3" evidence="2">
        <location>
            <begin position="49"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF96008.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="304" mass="34567" checksum="BE1C22628FEE2DED" modified="2011-07-27" version="1">MKCSKCGKEAIYHQKYSGLYLCKECFIKDVERKVRKVLGRNIIRNNMKIGVGLSGGKDSVVMTYILNKFFKPIPNSEIIVFFVDEGIKGFRKVARKFVVEFCEEYGIKYKIIPLEEEIGVSLDDIVKIAREKNLTLNPCSFCGVMRRKLLNRVALKEGCNYLAIGHNLDDMAQAVMMNYIDGAIEKLVRLGKDVEHPLLVKRIRPLKYVPEEEVQLYADLVGLKYQKEPCPYSSISYRAEISEIIDKLEDSHPGTMHSIVSGFEKLIKHLGIKMEVKTCKICGEPASGDICKVCLWMEKLGLKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBL8</accession>
    <name>F6BBL8_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Molybdopterin dinucleotide-binding region</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0471</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96027.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798635.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0471"/>
    <dbReference type="EnsemblBacteria" id="AEF96027">
        <property type="protein sequence ID" value="AEF96027"/>
        <property type="gene ID" value="Metig_0471"/>
    </dbReference>
    <dbReference type="GeneID" id="10643308"/>
    <dbReference type="KEGG" id="mig:Metig_0471"/>
    <dbReference type="HOGENOM" id="CLU_123704_1_0_2"/>
    <dbReference type="OMA" id="GMIYIPM"/>
    <dbReference type="OrthoDB" id="91239at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043546">
        <property type="term" value="F:molybdopterin cofactor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009010">
        <property type="entry name" value="Asp_de-COase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012040">
        <property type="entry name" value="Formylmethanofuran_DH_dsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006657">
        <property type="entry name" value="MoPterin_dinucl-bd_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01568">
        <property type="entry name" value="Molydop_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015873">
        <property type="entry name" value="FwdD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50692">
        <property type="entry name" value="SSF50692"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Molydop_binding" evidence="1">
        <location>
            <begin position="4"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96027.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="128" mass="14497" checksum="AADF471B9A7C8064" modified="2011-07-27" version="1">MKKFYLNTGRTIWQGEAIEAGKNLDLYVKAAAVCYINEDEMKDLGLKEGDKIKVKSEYGEVVVFAKKAREPMPKGMVYIPMGPWANKIVCPETDSTAMPSLKGPVLVEIEKTDEEFLDMPKLMRTYLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBL9</accession>
    <name>F6BBL9_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Formylmethanofuran dehydrogenase subunit A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0472</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96028.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798636.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0472"/>
    <dbReference type="EnsemblBacteria" id="AEF96028">
        <property type="protein sequence ID" value="AEF96028"/>
        <property type="gene ID" value="Metig_0472"/>
    </dbReference>
    <dbReference type="GeneID" id="10643309"/>
    <dbReference type="KEGG" id="mig:Metig_0472"/>
    <dbReference type="HOGENOM" id="CLU_035587_0_0_2"/>
    <dbReference type="OMA" id="GEKMDIC"/>
    <dbReference type="OrthoDB" id="8152at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016810">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01304">
        <property type="entry name" value="FMDH_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.40.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013108">
        <property type="entry name" value="Amidohydro_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012027">
        <property type="entry name" value="Formylmethanofuran_DH_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011059">
        <property type="entry name" value="Metal-dep_hydrolase_composite"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07969">
        <property type="entry name" value="Amidohydro_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006453">
        <property type="entry name" value="FwdA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51338">
        <property type="entry name" value="SSF51338"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="SSF51556"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03121">
        <property type="entry name" value="one_C_dehyd_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidohydro_3" evidence="1">
        <location>
            <begin position="40"/>
            <end position="499"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96028.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="567" mass="63931" checksum="721755983F87E6B5" modified="2011-07-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBQ1</accession>
    <name>F6BBQ1_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_0504</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96060.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798668.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0504"/>
    <dbReference type="EnsemblBacteria" id="AEF96060">
        <property type="protein sequence ID" value="AEF96060"/>
        <property type="gene ID" value="Metig_0504"/>
    </dbReference>
    <dbReference type="GeneID" id="10643341"/>
    <dbReference type="KEGG" id="mig:Metig_0504"/>
    <dbReference type="HOGENOM" id="CLU_598020_0_0_2"/>
    <dbReference type="OMA" id="DFAFGWP"/>
    <dbReference type="OrthoDB" id="44371at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF96060.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="447" mass="52290" checksum="EC471064ED6E9649" modified="2011-07-27" version="1">MEVQIYLGRGKALGWRKHGNKTLRKLSIKTEEANHTLFIGGSGFGKTTVMRGMIEAIWAAYTEKGIYPLIFVFERKIDVSKAEKIKEIYYIETQKYPRAQCMKKYGEKTWKYVEWYIQQMKKYPHLYGSPGDFAMGMPNVLGKYTRLSGDNSILGYFGLEPKAYPVTRFVFRPRRRLNNIKADNGWKTEVIDAKIKYSNINYAFLENLTNVGTGTLHGERLRKIWDIEGIRDPDKVLEIALQHEKNPDNPSKTYMRIEETMDRLKKDRLFSSDESFFKHISPKRINIIDFSQNSDLTHEEENLIFKMIVEWAIKVAFKYKIPVFFFIDEIQNFITNQHGKYAITKIYREGRSLGINLFGATQFLTGLDKILIDGAMHIAIVGKVVRLDDAKLLANMVPNLDIDDIMMEECNSIDEYLKIKQKNKFRGYFIYDKQFCERITYRHPQSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBQ9</accession>
    <name>F6BBQ9_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">4-hydroxy-tetrahydrodipicolinate reductase</fullName>
            <shortName evidence="9">HTPA reductase</shortName>
            <ecNumber evidence="9">1.17.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">dapB</name>
        <name evidence="12" type="ordered locus">Metig_1657</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the conversion of 4-hydroxy-tetrahydrodipicolinate (HTPA) to tetrahydrodipicolinate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>(S)-2,3,4,5-tetrahydrodipicolinate + H2O + NAD(+) = (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + H(+) + NADH</text>
            <dbReference type="Rhea" id="RHEA:35323"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16845"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:67139"/>
            <dbReference type="EC" id="1.17.1.8"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>(S)-2,3,4,5-tetrahydrodipicolinate + H2O + NADP(+) = (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + H(+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:35331"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16845"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:67139"/>
            <dbReference type="EC" id="1.17.1.8"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="9">Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; (S)-tetrahydrodipicolinate from L-aspartate: step 4/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the DapB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Was originally thought to be a dihydrodipicolinate reductase (DHDPR), catalyzing the conversion of dihydrodipicolinate to tetrahydrodipicolinate. However, it was shown in E.coli that the substrate of the enzymatic reaction is not dihydrodipicolinate (DHDP) but in fact (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinic acid (HTPA), the product released by the DapA-catalyzed reaction.</text>
    </comment>
    <dbReference type="EC" id="1.17.1.8" evidence="9"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97189.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799780.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1657"/>
    <dbReference type="EnsemblBacteria" id="AEF97189">
        <property type="protein sequence ID" value="AEF97189"/>
        <property type="gene ID" value="Metig_1657"/>
    </dbReference>
    <dbReference type="GeneID" id="10644530"/>
    <dbReference type="KEGG" id="mig:Metig_1657"/>
    <dbReference type="HOGENOM" id="CLU_047479_2_1_2"/>
    <dbReference type="OMA" id="HHPNKAD"/>
    <dbReference type="OrthoDB" id="86833at2157"/>
    <dbReference type="UniPathway" id="UPA00034">
        <property type="reaction ID" value="UER00018"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008839">
        <property type="term" value="F:4-hydroxy-tetrahydrodipicolinate reductase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016726">
        <property type="term" value="F:oxidoreductase activity, acting on CH or CH2 groups, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019877">
        <property type="term" value="P:diaminopimelate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009089">
        <property type="term" value="P:lysine biosynthetic process via diaminopimelate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00102">
        <property type="entry name" value="DapB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022663">
        <property type="entry name" value="DapB_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000846">
        <property type="entry name" value="DapB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022664">
        <property type="entry name" value="DapB_N_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023940">
        <property type="entry name" value="DHDPR_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20836">
        <property type="entry name" value="PTHR20836"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05173">
        <property type="entry name" value="DapB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01113">
        <property type="entry name" value="DapB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000161">
        <property type="entry name" value="DHPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00036">
        <property type="entry name" value="dapB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01298">
        <property type="entry name" value="DAPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="2 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 9" id="KW-0220">Diaminopimelate biosynthesis</keyword>
    <keyword evidence="8 9" id="KW-0457">Lysine biosynthesis</keyword>
    <keyword evidence="7 9" id="KW-0520">NAD</keyword>
    <keyword evidence="4 9" id="KW-0521">NADP</keyword>
    <keyword evidence="6 9 12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DapB_N" evidence="10">
        <location>
            <begin position="2"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="domain" description="DapB_C" evidence="11">
        <location>
            <begin position="134"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="region of interest" description="(S)-2,3,4,5-tetrahydrodipicolinate binding" evidence="9">
        <location>
            <begin position="170"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="9">
        <location>
            <position position="160"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="9">
        <location>
            <position position="164"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="8"/>
            <end position="13"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="34"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="102"/>
            <end position="104"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="128"/>
            <end position="131"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="161"/>
        </location>
        <ligand>
            <name>(S)-2,3,4,5-tetrahydrodipicolinate</name>
            <dbReference type="ChEBI" id="CHEBI:16845"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF05173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AEF97189.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="274" mass="30200" checksum="7B5A2A35AA4ADEC1" modified="2011-07-27" version="1">MIRVAVTGALGRMGSNIIKTICEQEDMEVVAAIEVPNHPKKGEDVGELIGIGKIGVPLSTSDELDKVLKETKPDVLVDFTIAHACVETVKIATKNKVKLVIGTTGFTEEQKKEIENAIKENKVSAVISQNFAIGVNIFFKTLEFLAKKLGHYDIEIVEMHHRFKKDAPSGTALRAAEIIKENREIPSEYVFGRYGMVGERKKEEIGIHALRGGDVVGDHTVIFAGDGERIELTHKASSRQAFVNGVILAIRFIVNKEEGIYNTFDVLGLNDIKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBR6</accession>
    <name>F6BBR6_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Cation efflux protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Metig_1664</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97196.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1664"/>
    <dbReference type="EnsemblBacteria" id="AEF97196">
        <property type="protein sequence ID" value="AEF97196"/>
        <property type="gene ID" value="Metig_1664"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_1664"/>
    <dbReference type="HOGENOM" id="CLU_1363650_0_0_2"/>
    <dbReference type="OMA" id="EICKVAY"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008324">
        <property type="term" value="F:cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1510.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002524">
        <property type="entry name" value="Cation_efflux"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027469">
        <property type="entry name" value="Cation_efflux_TMD_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01545">
        <property type="entry name" value="Cation_efflux"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161111">
        <property type="entry name" value="SSF161111"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01297">
        <property type="entry name" value="CDF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="40"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="82"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="112"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="154"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEF97196.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="200" mass="22040" checksum="A1100BC9B87514C1" modified="2011-07-27" version="1">MDKAIDLKKGERVAIISAILTFTIALIKGIVGYISGSIALIADALHSFSDVLGPIAVYVGLKFAQKDPNERFPYGYYRAETLASLVVSFLIFITGIETLRESIERISNPTTISHPFAAILALLISLAVTYYLYKYKERVGKEIHSQALMGEGQHSLVDFYTSVAALIAIISSYFGFTIIEPIVEAIISIAFRNCYILDEC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBU0</accession>
    <name>F6BBU0_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">N-(5'-phosphoribosyl)anthranilate isomerase</fullName>
            <shortName evidence="4">PRAI</shortName>
            <ecNumber evidence="4">5.3.1.24</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">trpF</name>
        <name evidence="6" type="ordered locus">Metig_1688</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 4">
            <text>N-(5-phospho-beta-D-ribosyl)anthranilate = 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate</text>
            <dbReference type="Rhea" id="RHEA:21540"/>
            <dbReference type="ChEBI" id="CHEBI:18277"/>
            <dbReference type="ChEBI" id="CHEBI:58613"/>
            <dbReference type="EC" id="5.3.1.24"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 4">Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 3/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 4">Belongs to the TrpF family.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.24" evidence="4"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97220.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799810.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1688"/>
    <dbReference type="EnsemblBacteria" id="AEF97220">
        <property type="protein sequence ID" value="AEF97220"/>
        <property type="gene ID" value="Metig_1688"/>
    </dbReference>
    <dbReference type="GeneID" id="10644561"/>
    <dbReference type="KEGG" id="mig:Metig_1688"/>
    <dbReference type="HOGENOM" id="CLU_076364_2_1_2"/>
    <dbReference type="OMA" id="FYAKSPR"/>
    <dbReference type="OrthoDB" id="117266at2157"/>
    <dbReference type="UniPathway" id="UPA00035">
        <property type="reaction ID" value="UER00042"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004640">
        <property type="term" value="F:phosphoribosylanthranilate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000162">
        <property type="term" value="P:tryptophan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00405">
        <property type="entry name" value="PRAI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00135">
        <property type="entry name" value="PRAI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001240">
        <property type="entry name" value="PRAI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044643">
        <property type="entry name" value="TrpF_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42894">
        <property type="entry name" value="PTHR42894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00697">
        <property type="entry name" value="PRAI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="SSF51366"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="4" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="4 6" id="KW-0413">Isomerase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0822">Tryptophan biosynthesis</keyword>
    <feature type="domain" description="PRAI" evidence="5">
        <location>
            <begin position="4"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEF97220.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="221" mass="24957" checksum="0CBA36E4290DCCBC" modified="2011-07-27" version="1">MVKVKICGITNKEDMAYISQKVHAVGMIIDVPVETPRKISLSKAVELKKYVCPFTSIVAVMMPESIEEVLRVENALKPNAIQLHGHESVKFLKKLKNLRENGEINSKIIKVLHVPKENPNLDNLIKTAKEYEKYCDALLVDTKIERIKIEGKTHDWEISKTIRESIEKPLILAGGLNKDNVKKAIEIVKPFAIDVSSSLESEKGKKDFKKVDEFLDVVKAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBU1</accession>
    <name>F6BBU1_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Sulfopyruvate decarboxylase, alpha subunit</fullName>
            <ecNumber evidence="2">4.1.1.79</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1689</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EC" id="4.1.1.79" evidence="2"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97221.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1689"/>
    <dbReference type="EnsemblBacteria" id="AEF97221">
        <property type="protein sequence ID" value="AEF97221"/>
        <property type="gene ID" value="Metig_1689"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_1689"/>
    <dbReference type="HOGENOM" id="CLU_113594_0_0_2"/>
    <dbReference type="OMA" id="IPMGQWT"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050545">
        <property type="term" value="F:sulfopyruvate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006082">
        <property type="term" value="P:organic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901576">
        <property type="term" value="P:organic substance biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044272">
        <property type="term" value="P:sulfur compound biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022502">
        <property type="entry name" value="Sulfopyruvate_deCO2ase_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012001">
        <property type="entry name" value="Thiamin_PyroP_enz_TPP-bd_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02776">
        <property type="entry name" value="TPP_enzyme_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="SSF52518"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03845">
        <property type="entry name" value="sulfopyru_alph"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0456">Lyase</keyword>
    <keyword evidence="2" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TPP_enzyme_N" evidence="1">
        <location>
            <begin position="43"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF97221.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="210" mass="23233" checksum="66A85DA2D1FD8EF9" modified="2011-07-27" version="1">MKPTIRPELQNPEGICLIGAPKERRAAKSVRISLNFKSFGENMKASESVYKAIKKSGIDFIASVPCANLKNLLNYIYNDKEILHVPVTREEEGFGVCAGAHLAGRKTALLMQNSGLGNSINAIGSLCKVYGIPLLLIISHRGDLKEKIPAQIPMGQWTKKLLDVVGIPYYCPKTPEEAYKIIPYASEFSIKMGYPVAVLLDALYWEYDSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBU3</accession>
    <name>F6BBU3_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Agmatinase</fullName>
            <ecNumber evidence="5">3.5.3.11</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Metig_1691</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the arginase family. Agmatinase subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.5.3.11" evidence="5"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97223.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799813.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1691"/>
    <dbReference type="EnsemblBacteria" id="AEF97223">
        <property type="protein sequence ID" value="AEF97223"/>
        <property type="gene ID" value="Metig_1691"/>
    </dbReference>
    <dbReference type="GeneID" id="10644564"/>
    <dbReference type="KEGG" id="mig:Metig_1691"/>
    <dbReference type="HOGENOM" id="CLU_039478_0_2_2"/>
    <dbReference type="OMA" id="YELTTIM"/>
    <dbReference type="OrthoDB" id="52310at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008783">
        <property type="term" value="F:agmatinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901564">
        <property type="term" value="P:organonitrogen compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005925">
        <property type="entry name" value="Agmatinase-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006035">
        <property type="entry name" value="Ureohydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023696">
        <property type="entry name" value="Ureohydrolase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020855">
        <property type="entry name" value="Ureohydrolase_Mn_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11358">
        <property type="entry name" value="PTHR11358"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00491">
        <property type="entry name" value="Arginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036979">
        <property type="entry name" value="Arginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52768">
        <property type="entry name" value="SSF52768"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01230">
        <property type="entry name" value="agmatinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01053">
        <property type="entry name" value="ARGINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51409">
        <property type="entry name" value="ARGINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEF97223.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="281" mass="31905" checksum="9F7BE33D8E984DE8" modified="2011-07-27" version="1">MEYFEDYTKFICASDDFENADAVIFGIPYDGTTSFKAGTRFGPKAIREASWGLETYSPILRKDLVDCNFCDMQDIFIYGSQEETFERIYQASKDIMKSEKIPIMLGGEHSVTYPVVKAAKDIYGDLVVLHFDAHCDLRDEYLGNKLSHACVIRRCYELTKDIYQFGIRSGDREEWEFAKNTKLSMELMSKEDVKEIKDLDKPIYLTVDIDVLDPAFAPGTGTPEPCGFSTRELLNSLYNLKEVNDRIIGFDVVEVSPHYDIGGITAIAAAKIVRELILMIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBW9</accession>
    <name>F6BBW9_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_1717</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97249.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799838.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF97249">
        <property type="protein sequence ID" value="AEF97249"/>
        <property type="gene ID" value="Metig_1717"/>
    </dbReference>
    <dbReference type="GeneID" id="10644591"/>
    <dbReference type="KEGG" id="mig:Metig_1717"/>
    <dbReference type="HOGENOM" id="CLU_157714_0_0_2"/>
    <dbReference type="OMA" id="IIRKMRN"/>
    <dbReference type="OrthoDB" id="122071at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090529">
        <property type="term" value="P:cell septum assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.110.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007561">
        <property type="entry name" value="Cell_div_SepF/SepF-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038594">
        <property type="entry name" value="SepF-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04472">
        <property type="entry name" value="SepF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF97249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="141" mass="16001" checksum="5FB6511FF8312D04" modified="2011-07-27" version="1">MVLEKLKEILGVGKPNIKQPAPLSIEEYVELPIASVEEEKPIRIKVCDLNDEKDAVNIIVMIEAGYLVIANTPHLERDIDEDYAAILSKLKNEVAKIGGKMVRLCETKLLIVPKNIVIEKIIKEEKEKKDEKKENNDNKDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-06-02" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBX1</accession>
    <name>F6BBX1_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Haloacid dehalogenase domain protein hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_1719</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97251.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799840.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF97251">
        <property type="protein sequence ID" value="AEF97251"/>
        <property type="gene ID" value="Metig_1719"/>
    </dbReference>
    <dbReference type="GeneID" id="10644593"/>
    <dbReference type="KEGG" id="mig:Metig_1719"/>
    <dbReference type="HOGENOM" id="CLU_1052165_0_0_2"/>
    <dbReference type="OMA" id="GISYCNP"/>
    <dbReference type="OrthoDB" id="66892at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="SSF56784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF97251.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="268" mass="30275" checksum="8D9752054CF92903" modified="2011-07-27" version="1">MKLAVVFDSAGTLVKVKRIVKEIKTQKFLCDRLTVDIVDEKVGRALVIIKDDPLEVIDKEDPEKLITELLKEVNWGISYCNKPIDKEGIFKDKKTKVKELQDTLNIMKRFKIQTGYGSAIIVDTLSGVIEYTITTGGCVFPEVPETIRKLKELGVKVFIASGDRKEFIKRLAELTGVDEKYIMPEAHQEQKRDLVLNLKKEGYYVIMVGDAANDVPAMLESDLSVVTLQNGTVSKKALETAKIRIYNIKEIVDICEDLIKIKKAKKNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBZ4</accession>
    <name>F6BBZ4_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcriptional regulator, HxlR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0520</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96075.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798683.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96075">
        <property type="protein sequence ID" value="AEF96075"/>
        <property type="gene ID" value="Metig_0520"/>
    </dbReference>
    <dbReference type="GeneID" id="10643357"/>
    <dbReference type="KEGG" id="mig:Metig_0520"/>
    <dbReference type="HOGENOM" id="CLU_153628_0_0_2"/>
    <dbReference type="OMA" id="VNLPKNY"/>
    <dbReference type="OrthoDB" id="130706at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000835">
        <property type="entry name" value="HTH_MarR-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01047">
        <property type="entry name" value="MarR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH marR-type" evidence="1">
        <location>
            <begin position="16"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96075.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="111" mass="12827" checksum="02CF5020169B248B" modified="2011-07-27" version="1">MENLLKLLAKKHVKEILQYLDTHGEVHFGQLHKDLNIHKGTLGNILDELVESEILNKRREETDALLPKTYYSLTNFGKRVLMVYKLCNALKKEDDTPIKIIYGDKVIASSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBZ9</accession>
    <name>F6BBZ9_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Integrase family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Metig_0525</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96080.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798688.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0525"/>
    <dbReference type="EnsemblBacteria" id="AEF96080">
        <property type="protein sequence ID" value="AEF96080"/>
        <property type="gene ID" value="Metig_0525"/>
    </dbReference>
    <dbReference type="GeneID" id="10643362"/>
    <dbReference type="KEGG" id="mig:Metig_0525"/>
    <dbReference type="HOGENOM" id="CLU_027562_9_5_2"/>
    <dbReference type="OMA" id="RIEVKHY"/>
    <dbReference type="OrthoDB" id="57897at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.443.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044068">
        <property type="entry name" value="CB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011010">
        <property type="entry name" value="DNA_brk_join_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013762">
        <property type="entry name" value="Integrase-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002104">
        <property type="entry name" value="Integrase_catalytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010998">
        <property type="entry name" value="Integrase_recombinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004107">
        <property type="entry name" value="Integrase_SAM-like_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13495">
        <property type="entry name" value="Phage_int_SAM_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00589">
        <property type="entry name" value="Phage_integrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56349">
        <property type="entry name" value="SSF56349"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51900">
        <property type="entry name" value="CB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51898">
        <property type="entry name" value="TYR_RECOMBINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="1 3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Core-binding (CB)" evidence="5">
        <location>
            <begin position="24"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="domain" description="Tyr recombinase" evidence="4">
        <location>
            <begin position="134"/>
            <end position="323"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEF96080.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="326" mass="39077" checksum="25DDE39BF4E42114" modified="2011-07-27" version="1">MGSEFNIKNLLLVKRKRDEIEETDEMRQWLERFKEEREFDGIKPSTIHNDLARLRVFLDFCYNRLGKSPDKLQTHDFVKFFNYLEVERKLSKNTQKRYYDLLKVFYRVLRMYPVIKGFVEESKDRKRFSRIEVKHYDPVDDRMLNLILERIIGSNSRVSIRNALIVRMLWDTGCRISEILNLKYRDCDFDSGVFRITDTKTHEERKVVCSEATLEALKWYVQFNVRQGPDDYIFQKQNGDKLSRDTITKVFNRVVKELKKEGKIPQNRRIVIHSLRHGRCVDLLEKGIPVDIVKEYLGHKTLDTTLFYSHSKERTEKLLKTIKKIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>F6BC13</accession>
    <name>F6BC13_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Transposase, IS605 OrfB family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Metig_0539</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96094.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0539"/>
    <dbReference type="EnsemblBacteria" id="AEF96094">
        <property type="protein sequence ID" value="AEF96094"/>
        <property type="gene ID" value="Metig_0539"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0539"/>
    <dbReference type="HOGENOM" id="CLU_032903_3_4_2"/>
    <dbReference type="OMA" id="PHIREGG"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032196">
        <property type="term" value="P:transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010095">
        <property type="entry name" value="Transposase_IS605_OrfB_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07282">
        <property type="entry name" value="OrfB_Zn_ribbon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01766">
        <property type="entry name" value="tspaseT_teng_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0815">Transposition</keyword>
    <feature type="domain" description="OrfB_Zn_ribbon" evidence="4">
        <location>
            <begin position="317"/>
            <end position="378"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF07282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEF96094.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="409" mass="48338" checksum="0143358898222E01" modified="2011-07-27" version="1">MQNKNKLPSEIVLTYKIKHNYDLKNLLDGFMSTSQKAVDIIWENINWKEKQVKHRFKSKNKYKYYTTTRLIPEIPKDKDFKRELRNYLLNGWNFASHYVDGAIKVAYSTIESWKSNYLDGKRKRNKPTFKRPFVRVKNTLIKYDKENGTIRITIKARKEYLVLDIKNEWFFEKIKGFDIGEIILKDNEALITFKKPLNLSDKKVVIGVDSNLKSLDLFHPEEGWIRIDLSELRRIKNVYDVIIDKLKSIYKKAPKRISILLKKYYNRRKNRVEDFINKLTSQLSKLFPNAIFIFEDLDKLNMYKNSNFNRDLDRTSWREIAKKLEYKSIVFYVNPHYTSKTCPVCGSKMESQEGQVVTCEKCGVFNRQFVGSFNIFKRGVKLVKKLLGGVGVLPERSDGNLKTVRFLCP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>F6BC21</accession>
    <name>F6BC21_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="3">ABC-type Fe3+-hydroxamate transport system periplasmic component-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_0548</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96102.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798709.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0548"/>
    <dbReference type="EnsemblBacteria" id="AEF96102">
        <property type="protein sequence ID" value="AEF96102"/>
        <property type="gene ID" value="Metig_0548"/>
    </dbReference>
    <dbReference type="GeneID" id="10643386"/>
    <dbReference type="KEGG" id="mig:Metig_0548"/>
    <dbReference type="HOGENOM" id="CLU_025776_0_0_2"/>
    <dbReference type="OMA" id="WIKFFGA"/>
    <dbReference type="OrthoDB" id="12936at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002491">
        <property type="entry name" value="ABC_transptr_periplasmic_BD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016134">
        <property type="entry name" value="Dockerin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036439">
        <property type="entry name" value="Dockerin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01497">
        <property type="entry name" value="Peripla_BP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63446">
        <property type="entry name" value="SSF63446"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51766">
        <property type="entry name" value="DOCKERIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50983">
        <property type="entry name" value="FE_B12_PBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dockerin" evidence="2">
        <location>
            <begin position="28"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="domain" description="Fe/B12 periplasmic-binding" evidence="1">
        <location>
            <begin position="153"/>
            <end position="425"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF96102.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="450" mass="51375" checksum="A0310E4C04B3BAA7" modified="2011-07-27" version="1">MPKNINTTGYILLTVFLLLVPVYAEGDYNYRLGDVNKDGSINIADVVYLFKHRDLNIEDGDLNADNSIDIADVVYLFVNYQKMREPIHYAQNIKITYYDENGNIVNPYNGESYAYKIVEDATGQRFLLKNETQPIPSWAEGKYDKVINVPLKKVVVMSSTHIALMEPLNNDGSVISSVKGIMWGGTYKWYFKDIEEGLNNGSIIDVGSSWNPNYDLIINISPQVVFVYPGYSGDKIIEKCKELNLTYVADAEYLENSYLARCEWVKMFAAFYNKEDVASKYFTRIEKRALNVKRLTRNCDRPLVAWGKNYPSYGGTYVPKAQSYVAKGIMEDCKGDYIFKDIPGTGSECISYETFAERAKDADIWVIPSSTAWLSNFKEDHPGYENFKAVQNGRLFCISGDYWQLGLMNTDEVLYDLATIIHPEVFKGRTTHYFLKYDPNTNTAEPYTAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>F6BC52</accession>
    <name>F6BC52_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Carbamoyltransferase</fullName>
            <ecNumber evidence="8">6.2.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">Metig_0579</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + C-terminal L-cysteinyl-[HypE protein] + carbamoyl phosphate + H2O = AMP + C-terminal S-carboxamide-L-cysteinyl-[HypE protein] + diphosphate + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:55636"/>
            <dbReference type="Rhea" id="RHEA-COMP:14247"/>
            <dbReference type="Rhea" id="RHEA-COMP:14392"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
            <dbReference type="ChEBI" id="CHEBI:76913"/>
            <dbReference type="ChEBI" id="CHEBI:139126"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>an acyl phosphate + H2O = a carboxylate + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:14965"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29067"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:59918"/>
            <dbReference type="EC" id="3.6.1.7"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; [NiFe] hydrogenase maturation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the carbamoyltransferase HypF family.</text>
    </comment>
    <dbReference type="EC" id="6.2.-.-" evidence="8"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96133.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798740.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0579"/>
    <dbReference type="EnsemblBacteria" id="AEF96133">
        <property type="protein sequence ID" value="AEF96133"/>
        <property type="gene ID" value="Metig_0579"/>
    </dbReference>
    <dbReference type="GeneID" id="10643417"/>
    <dbReference type="KEGG" id="mig:Metig_0579"/>
    <dbReference type="HOGENOM" id="CLU_009164_0_0_2"/>
    <dbReference type="OMA" id="VQHHYAH"/>
    <dbReference type="OrthoDB" id="6307at2157"/>
    <dbReference type="UniPathway" id="UPA00335"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003998">
        <property type="term" value="F:acylphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016743">
        <property type="term" value="F:carboxyl- or carbamoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003725">
        <property type="term" value="F:double-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046944">
        <property type="term" value="P:protein carbamoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001792">
        <property type="entry name" value="Acylphosphatase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036046">
        <property type="entry name" value="Acylphosphatase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017968">
        <property type="entry name" value="Acylphosphatase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004421">
        <property type="entry name" value="Carbamoyltransferase_HypF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017945">
        <property type="entry name" value="DHBP_synth_RibB-like_a/b_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041440">
        <property type="entry name" value="HypF_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006070">
        <property type="entry name" value="YrdC-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011125">
        <property type="entry name" value="Znf_HypF"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00708">
        <property type="entry name" value="Acylphosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17788">
        <property type="entry name" value="HypF_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01300">
        <property type="entry name" value="Sua5_yciO_yrdC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07503">
        <property type="entry name" value="zf-HYPF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006256">
        <property type="entry name" value="CMPcnvr_hdrg_mat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="SSF53067"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54975">
        <property type="entry name" value="SSF54975"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55821">
        <property type="entry name" value="SSF55821"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00143">
        <property type="entry name" value="hypF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00150">
        <property type="entry name" value="ACYLPHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51160">
        <property type="entry name" value="ACYLPHOSPHATASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51163">
        <property type="entry name" value="YRDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0436">Ligase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Acylphosphatase-like" evidence="10">
        <location>
            <begin position="3"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="domain" description="YrdC-like" evidence="11">
        <location>
            <begin position="202"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="active site" evidence="9">
        <location>
            <position position="18"/>
        </location>
    </feature>
    <feature type="active site" evidence="9">
        <location>
            <position position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004711"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR006256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AEF96133.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="762" mass="86910" checksum="33A1C970217EDD90" modified="2011-07-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>F6BC55</accession>
    <name>F6BC55_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Methanogenesis marker protein 5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_0582</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96136.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0582"/>
    <dbReference type="EnsemblBacteria" id="AEF96136">
        <property type="protein sequence ID" value="AEF96136"/>
        <property type="gene ID" value="Metig_0582"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0582"/>
    <dbReference type="HOGENOM" id="CLU_1567127_0_0_2"/>
    <dbReference type="OMA" id="GCARTNE"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012356">
        <property type="entry name" value="UCP018781_methan"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09885">
        <property type="entry name" value="DUF2112"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03271">
        <property type="entry name" value="methan_mark_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF96136.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="194" mass="21679" checksum="06DA953272906B85" modified="2011-07-27" version="1">MKEDLTYLNIIVLKNIAMKYEKFKNTNVYFALSIINFLGDKVKKVFIFPPNSLILGDLVERFGHKPLMLNNVIGQKVRTAEIDSPPLNITDEDPKKGLKYAAIEVPSGVRGRMSLIGPLIEEAEAAIIMKNAPIGFGCVGCERTNELTKYLIRRKGIPVLNVEYPKTEEEAKIVVKKIAAFLKELEEGDNGVSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>F6BC62</accession>
    <name>F6BC62_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Nitrogenase iron protein</fullName>
            <ecNumber evidence="12">1.18.6.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Nitrogenase Fe protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Nitrogenase component II</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Nitrogenase reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">nifH</name>
        <name evidence="15" type="ordered locus">Metig_1736</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 12">The key enzymatic reactions in nitrogen fixation are catalyzed by the nitrogenase complex, which has 2 components: the iron protein and the molybdenum-iron protein.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 12">
            <text>16 ATP + 16 H2O + N2 + 8 reduced [2Fe-2S]-[ferredoxin] = 16 ADP + 6 H(+) + H2 + 2 NH4(+) + 8 oxidized [2Fe-2S]-[ferredoxin] + 16 phosphate</text>
            <dbReference type="Rhea" id="RHEA:21448"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17997"/>
            <dbReference type="ChEBI" id="CHEBI:18276"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="1.18.6.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="12">Binds 1 [4Fe-4S] cluster per dimer.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4 12">Homodimer.</text>
    </comment>
    <comment type="PTM">
        <text evidence="13">The reversible ADP-ribosylation of Arg inactivates the nitrogenase reductase and regulates nitrogenase activity.</text>
    </comment>
    <comment type="PTM">
        <text evidence="12">The reversible ADP-ribosylation of Arg-108 inactivates the nitrogenase reductase and regulates nitrogenase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 12 14">Belongs to the NifH/BchL/ChlL family.</text>
    </comment>
    <dbReference type="EC" id="1.18.6.1" evidence="12"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97268.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799857.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1736"/>
    <dbReference type="EnsemblBacteria" id="AEF97268">
        <property type="protein sequence ID" value="AEF97268"/>
        <property type="gene ID" value="Metig_1736"/>
    </dbReference>
    <dbReference type="GeneID" id="10644610"/>
    <dbReference type="KEGG" id="mig:Metig_1736"/>
    <dbReference type="HOGENOM" id="CLU_059373_0_0_2"/>
    <dbReference type="OMA" id="TTMMDTL"/>
    <dbReference type="OrthoDB" id="66372at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018697">
        <property type="term" value="F:carbonyl sulfide nitrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016163">
        <property type="term" value="F:nitrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009399">
        <property type="term" value="P:nitrogen fixation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00533">
        <property type="entry name" value="NifH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030655">
        <property type="entry name" value="NifH/chlL_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000392">
        <property type="entry name" value="NifH/frxC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005977">
        <property type="entry name" value="Nitrogenase_Fe_NifH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42864">
        <property type="entry name" value="PTHR42864"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00142">
        <property type="entry name" value="Fer4_NifH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000363">
        <property type="entry name" value="Nitrogenase_iron"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01287">
        <property type="entry name" value="nifH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00746">
        <property type="entry name" value="NIFH_FRXC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00692">
        <property type="entry name" value="NIFH_FRXC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51026">
        <property type="entry name" value="NIFH_FRXC_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 14" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="7 12 13" id="KW-0013">ADP-ribosylation</keyword>
    <keyword evidence="8 12 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 12 14" id="KW-0408">Iron</keyword>
    <keyword evidence="10 12 14" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="5 12 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 12" id="KW-0535">Nitrogen fixation</keyword>
    <keyword evidence="6 12 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12 14 15" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="17"/>
            <end position="24"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="105"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="140"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="modified residue" description="ADP-ribosylarginine; by dinitrogenase reductase ADP-ribosyltransferase" evidence="12 13">
        <location>
            <position position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR605977-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU003688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="AEF97268.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="284" mass="31110" checksum="252ACA5E6DD98E18" modified="2011-07-27" version="1">MSFDEIAPNAKKVAIYGKGGIGKSTTTQNTAAALAYYYKLKGMIHGCDPKADSTRMILHGKPQETVMDVLREEGEEGVTLEKVRKVGFAGIYCVESGGPEPGVGCAGRGVITAVNLMRELGGYPDDLDFLFFDVLGDVVCGGFAMPLRDGLAKEIYIVSSGEMMALYAANNIAKGILKYAEQSGVRLGGIICNSRKVDGELELMEEFCDKLGTKLIHFIPRDNIVQKAEFNKMTVVEFAPDSNQAKEYKKLGKKIMDNDELVIPTPMTMDEMEKLVEKYGLYDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-06-02" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>F6BC70</accession>
    <name>F6BC70_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_1744</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97276.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799865.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF97276">
        <property type="protein sequence ID" value="AEF97276"/>
        <property type="gene ID" value="Metig_1744"/>
    </dbReference>
    <dbReference type="GeneID" id="10644618"/>
    <dbReference type="KEGG" id="mig:Metig_1744"/>
    <dbReference type="HOGENOM" id="CLU_1307833_0_0_2"/>
    <dbReference type="OMA" id="EHYLMKH"/>
    <dbReference type="OrthoDB" id="76717at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF97276.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="210" mass="24315" checksum="E1C3FAF50657B336" modified="2011-07-27" version="1">MRILFLGKELEGIDSYNVEDCMFLPFNAIDIAKYDLAVIYLPLFFKNLTSYSANQASVITILKDLKIKECLENKTNVVVIDPYFKNISKGRIIEHYLMKHLTRDEFTVKVEEGNEIEIKDEKFKPYVDLIEEYSHIYNIVDEDGKLGLVDSLSLISDYDTWNYEVLFSTKDGKPLGVRVYKNGSLYILHPPKEGFNDFMGLIDTIKDTIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>F6BC75</accession>
    <name>F6BC75_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">50S ribosomal protein L40e</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">rpl40e</name>
        <name evidence="5" type="ordered locus">Metig_1749</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the eukaryotic ribosomal protein eL40 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97281.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799870.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1749"/>
    <dbReference type="EnsemblBacteria" id="AEF97281">
        <property type="protein sequence ID" value="AEF97281"/>
        <property type="gene ID" value="Metig_1749"/>
    </dbReference>
    <dbReference type="GeneID" id="10644623"/>
    <dbReference type="KEGG" id="mig:Metig_1749"/>
    <dbReference type="HOGENOM" id="CLU_205640_0_0_2"/>
    <dbReference type="OrthoDB" id="127293at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1060.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00788">
        <property type="entry name" value="Ribosomal_L40e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038587">
        <property type="entry name" value="L40e_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001975">
        <property type="entry name" value="Ribosomal_L40e"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023657">
        <property type="entry name" value="Ribosomal_L40e_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011332">
        <property type="entry name" value="Ribosomal_zn-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39649">
        <property type="entry name" value="PTHR39649"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01020">
        <property type="entry name" value="Ribosomal_L40e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01377">
        <property type="entry name" value="Ribosomal_L40e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57829">
        <property type="entry name" value="SSF57829"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="1 3 5" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Ribosomal_L40e" evidence="4">
        <location>
            <begin position="3"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00788"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM01377"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEF97281.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="47" mass="5532" checksum="0A64510CCE92B477" modified="2011-07-27" version="1">MAFEEAVKRIFMKKICMRCNARNPWKATKCRKCGYKGLRPKAKEPRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>F6BC92</accession>
    <name>F6BC92_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Translation initiation factor 1A</fullName>
            <shortName evidence="3">aIF-1A</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">eif1a</name>
        <name evidence="8" type="ordered locus">Metig_1766</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 3 6">Seems to be required for maximal rate of protein biosynthesis. Enhances ribosome dissociation into subunits and stabilizes the binding of the initiator Met-tRNA(I) to 40 S ribosomal subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 3 5">Belongs to the eIF-1A family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97298.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799887.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1766"/>
    <dbReference type="EnsemblBacteria" id="AEF97298">
        <property type="protein sequence ID" value="AEF97298"/>
        <property type="gene ID" value="Metig_1766"/>
    </dbReference>
    <dbReference type="GeneID" id="10644640"/>
    <dbReference type="KEGG" id="mig:Metig_1766"/>
    <dbReference type="HOGENOM" id="CLU_109098_1_2_2"/>
    <dbReference type="OMA" id="ADITWRY"/>
    <dbReference type="OrthoDB" id="102432at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd05793">
        <property type="entry name" value="S1_IF1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00216">
        <property type="entry name" value="aIF_1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006196">
        <property type="entry name" value="RNA-binding_domain_S1_IF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001253">
        <property type="entry name" value="TIF_eIF-1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018104">
        <property type="entry name" value="TIF_eIF-1A_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21668">
        <property type="entry name" value="PTHR21668"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01176">
        <property type="entry name" value="eIF-1a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00652">
        <property type="entry name" value="eIF1a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00523">
        <property type="entry name" value="eIF-1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01262">
        <property type="entry name" value="IF1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50832">
        <property type="entry name" value="S1_IF1_TYPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4 6" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="3 4 6" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="S1-like" evidence="7">
        <location>
            <begin position="8"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00025502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU004364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEF97298.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="100" mass="11808" checksum="4830C559F8DCFB92" modified="2011-07-27" version="1">MQQQEQPIRVRIPKPEENEILGIVEQMLGASRVRVRCMDGKTRLGRIPGKLKKRIWIREGDVVIVVPWEVQGDEKCDIVWRYTKTQVEWLTKHGYLNQLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCD0</accession>
    <name>F6BCD0_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ATPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0587</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96141.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798748.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96141">
        <property type="protein sequence ID" value="AEF96141"/>
        <property type="gene ID" value="Metig_0587"/>
    </dbReference>
    <dbReference type="GeneID" id="10643425"/>
    <dbReference type="KEGG" id="mig:Metig_0587"/>
    <dbReference type="HOGENOM" id="CLU_068608_0_0_2"/>
    <dbReference type="OMA" id="FLEIMEY"/>
    <dbReference type="OrthoDB" id="36621at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011579">
        <property type="entry name" value="ATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01637">
        <property type="entry name" value="ATPase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATPase_2" evidence="1">
        <location>
            <begin position="3"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96141.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="361" mass="42745" checksum="F6ED3C1C321CC3D6" modified="2011-07-27" version="1">MKFFNREKEIKEILSILEGEPNSIYFTYGPLNSGKTTLIKHIIENELSDDYKVFYINFRTYLISEKREFIEAIFTTKKDDLFEKIKDKNEVLKLITKGAKILTGIPIPEVEFDKLFEEKINDAFQYLNSLLLAVKKSGKKPILILDELQMIKENRQKYLLKELFQFLVSLTKEQHLCHVFCLSSDSLFIEYVYNTGELEGRAKYILVDDFDKETALKFIDFLSENLLNKKLSDEDKELIFSYIGGKPLYLYNVIDEMRYKELKEILDLMLKEEVSKLDMFLEFLEYSKPKVKIKNEVIELKKEDIINALKLFKNKYEILKKEMPTPVYIYLVKENILFLDPIEGTLKPQSYLVWNAIKRIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCJ4</accession>
    <name>F6BCJ4_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">tRNA pseudouridine synthase Pus10</fullName>
            <ecNumber evidence="4">5.4.99.25</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">tRNA pseudouridine 54/55 synthase</fullName>
            <shortName evidence="4">Psi54/55 synthase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">pus10</name>
        <name evidence="7" type="ordered locus">Metig_0655</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Responsible for synthesis of pseudouridine from uracil-54 and uracil-55 in the psi GC loop of transfer RNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>uridine(54) in tRNA = pseudouridine(54) in tRNA</text>
            <dbReference type="Rhea" id="RHEA:57876"/>
            <dbReference type="Rhea" id="RHEA-COMP:10193"/>
            <dbReference type="Rhea" id="RHEA-COMP:14141"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>uridine(55) in tRNA = pseudouridine(55) in tRNA</text>
            <dbReference type="Rhea" id="RHEA:42532"/>
            <dbReference type="Rhea" id="RHEA-COMP:10101"/>
            <dbReference type="Rhea" id="RHEA-COMP:10102"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="EC" id="5.4.99.25"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the pseudouridine synthase Pus10 family.</text>
    </comment>
    <dbReference type="EC" id="5.4.99.25" evidence="4"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96205.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798808.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0655"/>
    <dbReference type="EnsemblBacteria" id="AEF96205">
        <property type="protein sequence ID" value="AEF96205"/>
        <property type="gene ID" value="Metig_0655"/>
    </dbReference>
    <dbReference type="GeneID" id="10643495"/>
    <dbReference type="KEGG" id="mig:Metig_0655"/>
    <dbReference type="HOGENOM" id="CLU_028780_2_0_2"/>
    <dbReference type="OMA" id="DGGLYIK"/>
    <dbReference type="OrthoDB" id="61936at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106029">
        <property type="term" value="F:tRNA pseudouridine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031119">
        <property type="term" value="P:tRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01893">
        <property type="entry name" value="Pus10_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005912">
        <property type="entry name" value="Pus10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039894">
        <property type="entry name" value="Pus10-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004114">
        <property type="entry name" value="THUMP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21568">
        <property type="entry name" value="PTHR21568"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02926">
        <property type="entry name" value="THUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="SSF55120"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01213">
        <property type="entry name" value="pseudo_Pus10arc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51165">
        <property type="entry name" value="THUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0413">Isomerase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2 4" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="THUMP" evidence="6">
        <location>
            <begin position="86"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="4">
        <location>
            <position position="261"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="325"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="397"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009652"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEF96205.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="437" mass="51109" checksum="42A3152A102E3A22" modified="2011-07-27" version="1">MEINIEILKKYPLCHRCFGRLYAKLLHTNNFERGKALKLVKAMELESLLKKIEESGEGNKDEVLELLKCLYRSGLKEIKIKEIKEGEKIEECPWCRGIFEKSHIEELANKAIELLKEYEFDTFMVGTHIPKEIKELEEEIETPYMESIRQEFGREIGKIIAVKMNKQPDKTNPDIVVHINPYTKDIYLQVNPLFIKGRYRKLVRGIPQTHWHCRFCRGKGCEKCNFTGKRYPTSVEEIIAEPFMKATKGTNEKFHGAGREDIDVRMLGNGRPFVLEIKEPKIRKIDLNKIAEEVNKSGMVEVLNLEYGNRKDVVFFKNEPHKKTYRALVECEEKITDEDIEKVIETCENLTIYQRTPKRVLHRRADLTRIRKVYKVWAHKIDDNHFEMKIFCDGGLYIKELISGDDGRTSPSISEILNKKCICKELDVLEVHDEETS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCK4</accession>
    <name>F6BCK4_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">FAD dependent oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0665</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96215.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798818.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0665"/>
    <dbReference type="EnsemblBacteria" id="AEF96215">
        <property type="protein sequence ID" value="AEF96215"/>
        <property type="gene ID" value="Metig_0665"/>
    </dbReference>
    <dbReference type="GeneID" id="10643505"/>
    <dbReference type="KEGG" id="mig:Metig_0665"/>
    <dbReference type="HOGENOM" id="CLU_052500_0_0_2"/>
    <dbReference type="OMA" id="CDAKWIP"/>
    <dbReference type="OrthoDB" id="43345at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016614">
        <property type="term" value="F:oxidoreductase activity, acting on CH-OH group of donors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007867">
        <property type="entry name" value="GMC_OxRtase_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05199">
        <property type="entry name" value="GMC_oxred_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="111"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96215.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="387" mass="43162" checksum="B5B338A2D49D78C1" modified="2011-07-27" version="1">MFDFAIIGSGAAGATLAKELSTDAKVAVLEKGEHYPKYVIEGNFVDIAYLNSIGGSTVCAVGNALRVKIKGIKIEDEIYEEIERELNVKRPPYEFLREYAKKFLNLGFERTPKYINFDKCNKCGKCAHKPCNAKWTAAEFIKESKADVICGFEVKNIRKEGDVFYIDGVDKNTNSLRTIKAKNVIISAGGINSPRILSKILDNEHIGKNLFVDTFITVGGILKDTYLNKDVQMIVYKKYRDFILASHYSELLYSRINEYEKTRKEDVIGIMIKIKDENVGEVGEDYVSKPMTKKDTQILSEGVGEASKILHEFGVERIYSTIPRGSHPGGTCAIKKVVDENLETEIEGLYVCDASVFPEAPGAPPILAIIGIAKKLARYLKNEKMGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCL9</accession>
    <name>F6BCL9_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Nucleoside recognition domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0680</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96230.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0680"/>
    <dbReference type="EnsemblBacteria" id="AEF96230">
        <property type="protein sequence ID" value="AEF96230"/>
        <property type="gene ID" value="Metig_0680"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0680"/>
    <dbReference type="HOGENOM" id="CLU_3362561_0_0_2"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96230.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="35" mass="3757" checksum="CA92B233780C4A51" modified="2011-07-27" version="1">MGIISYLVKIVILSSIGIVVAGIIEETNLLSKIKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCT1</accession>
    <name>F6BCT1_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Multiple resistance and pH regulation protein F</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0744</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96292.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798895.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0744"/>
    <dbReference type="EnsemblBacteria" id="AEF96292">
        <property type="protein sequence ID" value="AEF96292"/>
        <property type="gene ID" value="Metig_0744"/>
    </dbReference>
    <dbReference type="GeneID" id="10643583"/>
    <dbReference type="KEGG" id="mig:Metig_0744"/>
    <dbReference type="HOGENOM" id="CLU_189621_0_0_2"/>
    <dbReference type="OMA" id="FAFCKVI"/>
    <dbReference type="OrthoDB" id="115824at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="37"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="62"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96292.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="93" mass="9922" checksum="F70BA12C5F72D56D" modified="2011-07-27" version="1">MHEYVIYFTLFSLAVGALASLKLSFKKNTANVLVGEGLLAVIVATFIVVVSNYYGISFGETIALLLLVSGPIGTIAFSKIMKKSNSISNSNKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-06-02" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCT7</accession>
    <name>F6BCT7_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_0751</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96298.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798901.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96298">
        <property type="protein sequence ID" value="AEF96298"/>
        <property type="gene ID" value="Metig_0751"/>
    </dbReference>
    <dbReference type="GeneID" id="10643590"/>
    <dbReference type="KEGG" id="mig:Metig_0751"/>
    <dbReference type="HOGENOM" id="CLU_114816_0_0_2"/>
    <dbReference type="OMA" id="PNEGRYR"/>
    <dbReference type="OrthoDB" id="112404at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF96298.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="163" mass="19464" checksum="FF04C9653F64390F" modified="2011-07-27" version="1">MGFKCEEFKEIADNLPNFKSLPNEGRYRAAIGRYYYYTFLTVRDMIYRVDKREDIEKYYSSGLIHSFIRIYLNKFSKNIGSDELNIASMLLSKLHFLRKLADYNTNEKITYKHVLRAKRYSQSIIEILNNLEYNGVVGFETILKHLKEHGKEEGDEYKYFPRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCU7</accession>
    <name>F6BCU7_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Formate dehydrogenase, alpha subunit</fullName>
            <ecNumber evidence="9">1.7.99.4</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Metig_0762</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mo-bis(molybdopterin guanine dinucleotide)</name>
            <dbReference type="ChEBI" id="CHEBI:60539"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <dbReference type="EC" id="1.7.99.4" evidence="9"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96308.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0762"/>
    <dbReference type="KEGG" id="mig:Metig_0762"/>
    <dbReference type="HOGENOM" id="CLU_000422_4_0_2"/>
    <dbReference type="OMA" id="DGPPCCY"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008863">
        <property type="term" value="F:formate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043546">
        <property type="term" value="F:molybdopterin cofactor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008940">
        <property type="term" value="F:nitrate reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015942">
        <property type="term" value="P:formate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02790">
        <property type="entry name" value="MopB_CT_Formate-Dh_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02753">
        <property type="entry name" value="MopB_Formate-Dh-H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009010">
        <property type="entry name" value="Asp_de-COase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041925">
        <property type="entry name" value="CT_Formate-Dh_H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041924">
        <property type="entry name" value="Formate_Dh-H_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006478">
        <property type="entry name" value="Formate_DH_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006657">
        <property type="entry name" value="MoPterin_dinucl-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006656">
        <property type="entry name" value="Mopterin_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006963">
        <property type="entry name" value="Mopterin_OxRdtase_4Fe-4S_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006655">
        <property type="entry name" value="Mopterin_OxRdtase_prok_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027467">
        <property type="entry name" value="MopterinOxRdtase_cofactor_BS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04879">
        <property type="entry name" value="Molybdop_Fe4S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00384">
        <property type="entry name" value="Molybdopterin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01568">
        <property type="entry name" value="Molydop_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00926">
        <property type="entry name" value="Molybdop_Fe4S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50692">
        <property type="entry name" value="SSF50692"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01591">
        <property type="entry name" value="Fdh-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51669">
        <property type="entry name" value="4FE4S_MOW_BIS_MGD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00551">
        <property type="entry name" value="MOLYBDOPTERIN_PROK_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00490">
        <property type="entry name" value="MOLYBDOPTERIN_PROK_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00932">
        <property type="entry name" value="MOLYBDOPTERIN_PROK_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="4 7" id="KW-0408">Iron</keyword>
    <keyword evidence="5 7" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0520">NAD</keyword>
    <keyword evidence="9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0711">Selenium</keyword>
    <keyword evidence="9" id="KW-0712">Selenocysteine</keyword>
    <feature type="domain" description="4Fe-4S Mo/W bis-MGD-type" evidence="8">
        <location>
            <begin position="3"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="10"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="13"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="17"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="45"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="non-standard amino acid" description="Selenocysteine" evidence="9">
        <location>
            <position position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AEF96308.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="692" mass="77469" checksum="0767AE30F81C169B" modified="2011-07-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCW8</accession>
    <name>F6BCW8_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Phosphoesterase PA-phosphatase related protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_0784</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96329.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798932.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0784"/>
    <dbReference type="EnsemblBacteria" id="AEF96329">
        <property type="protein sequence ID" value="AEF96329"/>
        <property type="gene ID" value="Metig_0784"/>
    </dbReference>
    <dbReference type="GeneID" id="10643624"/>
    <dbReference type="KEGG" id="mig:Metig_0784"/>
    <dbReference type="HOGENOM" id="CLU_777588_0_0_2"/>
    <dbReference type="OMA" id="PYKVIAW"/>
    <dbReference type="OrthoDB" id="58548at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036938">
        <property type="entry name" value="P_Acid_Pase_2/haloperoxi_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000326">
        <property type="entry name" value="P_Acid_Pase_2/haloperoxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032816">
        <property type="entry name" value="SNARE_assoc"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01569">
        <property type="entry name" value="PAP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09335">
        <property type="entry name" value="SNARE_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00014">
        <property type="entry name" value="acidPPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48317">
        <property type="entry name" value="SSF48317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="36"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="97"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="141"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="180"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="215"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="280"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="303"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="domain" description="acidPPc" evidence="2">
        <location>
            <begin position="211"/>
            <end position="320"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF96329.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="338" mass="37958" checksum="EF3FDEB21CF7A1CB" modified="2011-07-27" version="1">MDLYAIAEYLVHNYGYLGIFLVAFTEAFIQPVPPDIFIMGASLFGLNPLISALTATIGSLFGGLFGYFLGNKLGHPAFIKLFGDKYLIKGEEFFNKYGFWGVALAGFTPIPYKVIAWLAGIFEMSKFPFSIGTFVGRLPRFLAVAYFGNILVSFDYTALIETLNKINIQLFYTINSHYNVFLDMTMTIITHSAYPMAITVLVLSFLKDRNFGNKVLIALTLAFLVAFSLKYIINEPRPYLILKNIHLLSYEGYEPSFPSGHTTFAFTVSTLLYSYSKKMGLIFLIWAILVGYSRVYVGVHYPFDVLAGAIIGIVCGYLVVNKKIEKLLKLLGKYSNLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>F6BD02</accession>
    <name>F6BD02_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Cobalt ABC transporter, inner membrane subunit CbiQ</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Metig_0818</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96363.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0818"/>
    <dbReference type="EnsemblBacteria" id="AEF96363">
        <property type="protein sequence ID" value="AEF96363"/>
        <property type="gene ID" value="Metig_0818"/>
    </dbReference>
    <dbReference type="GeneID" id="10643659"/>
    <dbReference type="KEGG" id="mig:Metig_0818"/>
    <dbReference type="HOGENOM" id="CLU_056469_5_0_2"/>
    <dbReference type="OMA" id="IAMMMYR"/>
    <dbReference type="OrthoDB" id="51220at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005887">
        <property type="term" value="C:integral component of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006824">
        <property type="term" value="P:cobalt ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003339">
        <property type="entry name" value="ABC/ECF_trnsptr_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012809">
        <property type="entry name" value="ECF_CbiQ"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02361">
        <property type="entry name" value="CbiQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02454">
        <property type="entry name" value="ECF_T_CbiQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="23"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="63"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="110"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="234"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEF96363.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="259" mass="29692" checksum="C1F5893B168350AC" modified="2011-07-27" version="1">MQNIIDNIAHYNKLRMVNPKLKVIFAISSLLVCVFSKSIVVPIIVALIMIYLTLFRAGVPKSIYLKLMFAPIGFGLITLVLMAFMFGYVKLFSFEIFGFKIPIYKDGIDLGLLVFSRMLGGVACMLFLALTTPMTELFYVLRELGVPSSVLDIAMMMYRYIFVLLEEMIRVENAQKTRLGYRNLKTTFKSLGMLASNLFIRTWERGEQLFITMSSRCYNGDLKVFGKIENPKSIYIVGIILFEIFLVIISYLTMNFKPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>F6BD20</accession>
    <name>F6BD20_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0248 protein Metig_0836</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_0836</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0248 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96381.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798983.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96381">
        <property type="protein sequence ID" value="AEF96381"/>
        <property type="gene ID" value="Metig_0836"/>
    </dbReference>
    <dbReference type="GeneID" id="10643677"/>
    <dbReference type="KEGG" id="mig:Metig_0836"/>
    <dbReference type="HOGENOM" id="CLU_172276_3_0_2"/>
    <dbReference type="OMA" id="TQIPYHR"/>
    <dbReference type="OrthoDB" id="121890at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01245">
        <property type="entry name" value="UPF0248"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040459">
        <property type="entry name" value="MJ1316"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007547">
        <property type="entry name" value="UPF0248"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04457">
        <property type="entry name" value="MJ1316"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MJ1316" evidence="2">
        <location>
            <begin position="3"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF96381.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="97" mass="11759" checksum="02950F9A483347C1" modified="2011-07-27" version="1">MLKELINKILWHPKYNPEDFEIVYLHRGAENNKKVISMEKVISMDEVHIEGSFIVHDTTHIPFHRILEIRNKKTKEILFRKHDKDTNFVKEYENEDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>F6BD44</accession>
    <name>F6BD44_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">30S ribosomal protein S11</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">rps11</name>
        <name evidence="6" type="ordered locus">Metig_0861</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Located on the platform of the 30S subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Part of the 30S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4 5">Belongs to the universal ribosomal protein uS11 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96405.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799007.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0861"/>
    <dbReference type="EnsemblBacteria" id="AEF96405">
        <property type="protein sequence ID" value="AEF96405"/>
        <property type="gene ID" value="Metig_0861"/>
    </dbReference>
    <dbReference type="GeneID" id="10643704"/>
    <dbReference type="KEGG" id="mig:Metig_0861"/>
    <dbReference type="HOGENOM" id="CLU_072439_6_1_2"/>
    <dbReference type="OMA" id="KWGVAHI"/>
    <dbReference type="OrthoDB" id="114285at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01310">
        <property type="entry name" value="Ribosomal_S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001971">
        <property type="entry name" value="Ribosomal_S11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019961">
        <property type="entry name" value="Ribosomal_S11_archaeal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018102">
        <property type="entry name" value="Ribosomal_S11_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036967">
        <property type="entry name" value="Ribosomal_S11_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11759">
        <property type="entry name" value="PTHR11759"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00411">
        <property type="entry name" value="Ribosomal_S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002131">
        <property type="entry name" value="Ribosomal_S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03628">
        <property type="entry name" value="arch_S11P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00054">
        <property type="entry name" value="RIBOSOMAL_S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 5" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="4" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0699">rRNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEF96405.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="127" mass="13744" checksum="EF770EF46229084E" modified="2011-07-27" version="1">MAEKVERWGVVHIYASYNNTIIHVTDITGAETIARISGGMIVKNQRDEASPYAAMQAAFKIAEILKERGIEAVHVKVRAPGGHKSRTPGPGAQAAIRALARAGIRIGRIEDVTPIPHDGTTPKKSRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>F6BD48</accession>
    <name>F6BD48_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 5 7">30S ribosomal protein S9</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">rps9</name>
        <name evidence="9" type="ordered locus">Metig_0865</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5 6">Belongs to the universal ribosomal protein uS9 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96409.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799011.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0865"/>
    <dbReference type="EnsemblBacteria" id="AEF96409">
        <property type="protein sequence ID" value="AEF96409"/>
        <property type="gene ID" value="Metig_0865"/>
    </dbReference>
    <dbReference type="GeneID" id="10643708"/>
    <dbReference type="KEGG" id="mig:Metig_0865"/>
    <dbReference type="HOGENOM" id="CLU_046483_4_0_2"/>
    <dbReference type="OMA" id="FMGQAEA"/>
    <dbReference type="OrthoDB" id="115425at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00532_A">
        <property type="entry name" value="Ribosomal_S9_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000754">
        <property type="entry name" value="Ribosomal_S9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019958">
        <property type="entry name" value="Ribosomal_S9_archaeal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020574">
        <property type="entry name" value="Ribosomal_S9_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21569">
        <property type="entry name" value="PTHR21569"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00380">
        <property type="entry name" value="Ribosomal_S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03627">
        <property type="entry name" value="uS9_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00360">
        <property type="entry name" value="RIBOSOMAL_S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 5 6" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="110"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00016789"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AEF96409.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="135" mass="15135" checksum="A06619079FECFBB7" modified="2011-07-27" version="1">MKIVHTVGKRKTAIARATARPGKGRIRINKVPIELIEPKYKRMKLMEPIILAGEEVIKQMDIDVVVKGGGVMGQMDAARTAIGKAIVEFTGDPKIKEKFLAYDRTLLVSDARRTEPHKPSRSTKGPRAKRQKSYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDE1</accession>
    <name>F6BDE1_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0959</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CTAG/PCC1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96502.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0959"/>
    <dbReference type="EnsemblBacteria" id="AEF96502">
        <property type="protein sequence ID" value="AEF96502"/>
        <property type="gene ID" value="Metig_0959"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0959"/>
    <dbReference type="HOGENOM" id="CLU_170076_0_1_2"/>
    <dbReference type="OMA" id="YSYIRWI"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015419">
        <property type="entry name" value="CTAG/Pcc1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09341">
        <property type="entry name" value="Pcc1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96502.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="97" mass="11354" checksum="3FFDEAF23F70213E" modified="2011-07-27" version="1">MLNKLIMKILKWEKMSKINSFCLEIEFDSPEEAEVIYKAILLDHLESQIKSKADMQLNANILNIQIFAEDLSILRAALYSYLRWIKVSDNIYKICKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDE5</accession>
    <name>F6BDE5_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Dihydroneopterin aldolase</fullName>
            <shortName evidence="1">DHNA</shortName>
            <ecNumber evidence="1">4.1.2.25</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">7,8-dihydroneopterin aldolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">mptD</name>
        <name evidence="3" type="ordered locus">Metig_0963</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the conversion of 7,8-dihydroneopterin (H2Neo) to 6-hydroxymethyl-7,8-dihydropterin (6-HMD).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>7,8-dihydroneopterin = 6-hydroxymethyl-7,8-dihydropterin + glycolaldehyde</text>
            <dbReference type="Rhea" id="RHEA:10540"/>
            <dbReference type="ChEBI" id="CHEBI:17001"/>
            <dbReference type="ChEBI" id="CHEBI:17071"/>
            <dbReference type="ChEBI" id="CHEBI:44841"/>
            <dbReference type="EC" id="4.1.2.25"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; 5,6,7,8-tetrahydromethanopterin biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the archaeal dihydroneopterin aldolase family.</text>
    </comment>
    <dbReference type="EC" id="4.1.2.25" evidence="1"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96506.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799108.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0963"/>
    <dbReference type="EnsemblBacteria" id="AEF96506">
        <property type="protein sequence ID" value="AEF96506"/>
        <property type="gene ID" value="Metig_0963"/>
    </dbReference>
    <dbReference type="GeneID" id="10643809"/>
    <dbReference type="KEGG" id="mig:Metig_0963"/>
    <dbReference type="HOGENOM" id="CLU_149105_1_0_2"/>
    <dbReference type="OMA" id="FHQFVGT"/>
    <dbReference type="OrthoDB" id="110834at2157"/>
    <dbReference type="UniPathway" id="UPA00065"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004150">
        <property type="term" value="F:dihydroneopterin aldolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001118">
        <property type="term" value="P:tetrahydromethanopterin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1300.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02130">
        <property type="entry name" value="DHNA_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027508">
        <property type="entry name" value="DHN_aldolase_MptD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036839">
        <property type="entry name" value="MptD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007181">
        <property type="entry name" value="MtpD_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04038">
        <property type="entry name" value="DHNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143560">
        <property type="entry name" value="SSF143560"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DHNA" evidence="2">
        <location>
            <begin position="21"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="29"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="120"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF96506.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="126" mass="14521" checksum="0F1DE9C84763E0EC" modified="2011-07-27" version="1">MANIKKLEESEVFKSYFKNLTDRERAVFEGGITLGALFHQFVGTPVSLNNKESLEKAIEEAMKNQPCVEDIEVKIVGDFEEGKYVSLTGEMLNVKLKIRVNETIAILRLRYIEELDYPLMYVEKIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDE8</accession>
    <name>F6BDE8_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0966</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96509.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799111.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0966"/>
    <dbReference type="EnsemblBacteria" id="AEF96509">
        <property type="protein sequence ID" value="AEF96509"/>
        <property type="gene ID" value="Metig_0966"/>
    </dbReference>
    <dbReference type="GeneID" id="10643812"/>
    <dbReference type="KEGG" id="mig:Metig_0966"/>
    <dbReference type="HOGENOM" id="CLU_975258_0_0_2"/>
    <dbReference type="OMA" id="DMIFKDY"/>
    <dbReference type="OrthoDB" id="56379at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96509.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="272" mass="31151" checksum="30BC622C1FFF1DF6" modified="2011-07-27" version="1">MIKKILSKRGFIITYEAVIMVITFVSIFYVGYMAYTHNFLTFHEEKRNVEEIHHANLLADKILKDMEFPSNNYVADYHTFAKKVSEKYFGGEKARGTFDPFNIKKNNSTYVGFRPENLEITSNVNTTSINYTYIISNNNYVRTENLLVPVRTWGYANEKSIVENLTSGERLYFAVWDFSRLANITASSNTTTDAVFLVNGVTFNLTLNQTPKITGFGKVISTRSYEPNEIRVLDVSNNAIVYLNITYNQPSTIYVAKFKPYNISIIINYNGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDF8</accession>
    <name>F6BDF8_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Peptidase A24A domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metig_0976</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96519.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799121.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0976"/>
    <dbReference type="MEROPS" id="A24.018"/>
    <dbReference type="EnsemblBacteria" id="AEF96519">
        <property type="protein sequence ID" value="AEF96519"/>
        <property type="gene ID" value="Metig_0976"/>
    </dbReference>
    <dbReference type="GeneID" id="10643822"/>
    <dbReference type="KEGG" id="mig:Metig_0976"/>
    <dbReference type="HOGENOM" id="CLU_805645_0_0_2"/>
    <dbReference type="OMA" id="WKDIIPS"/>
    <dbReference type="OrthoDB" id="49068at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009639">
        <property type="entry name" value="Pept_A24A_C_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000045">
        <property type="entry name" value="Prepilin_IV_endopep_pep"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06819">
        <property type="entry name" value="Arc_PepC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01478">
        <property type="entry name" value="Peptidase_A24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="30"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="53"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="99"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="124"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="147"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="178"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="204"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="316"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_A24" evidence="2">
        <location>
            <begin position="10"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="domain" description="Arc_PepC" evidence="3">
        <location>
            <begin position="206"/>
            <end position="313"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF06819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEF96519.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="344" mass="38921" checksum="60AE4BE3A3CEF27D" modified="2011-07-27" version="1">MLFLSIAYVVNAILLLLASLTDIKERIIPHKYVIAMLILNLTVGYYYFGTDAIIAFFSTLILCLILSVGMGGGDVKVFSALAPLFAFDLVYYFPKYILVLIGLSAGLAVFFPMLKILKVYWRDILPSSLYLATVLGILVYITQIYNIPYATIVVWGYIILSIILSKKIPNYKEITKKLGFLFPIYLVGLYFVDTNYFISNNVFLSSLIYVGELTLISIVIYALMGAEVSDKKAIGELKEGDILRDIIVLKDNGDVVVENANLIKRIKFLIDYEKGGKKDYKLIFTDGEGLSEETIKLLKELHKNGKIPNELNVLKTYPFIPFVFLAYLIILIIMYKMNVGYYVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDJ1</accession>
    <name>F6BDJ1_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Sjogrens syndrome scleroderma autoantigen 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_1010</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96552.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799154.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1010"/>
    <dbReference type="EnsemblBacteria" id="AEF96552">
        <property type="protein sequence ID" value="AEF96552"/>
        <property type="gene ID" value="Metig_1010"/>
    </dbReference>
    <dbReference type="GeneID" id="10643864"/>
    <dbReference type="KEGG" id="mig:Metig_1010"/>
    <dbReference type="HOGENOM" id="CLU_142653_2_0_2"/>
    <dbReference type="OMA" id="AKMLGKH"/>
    <dbReference type="OrthoDB" id="99528at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009563">
        <property type="entry name" value="SSSCA1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06677">
        <property type="entry name" value="Auto_anti-p27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF96552.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="106" mass="12212" checksum="94C7477542164BEC" modified="2011-07-27" version="1">MDVVQLMSRELLKGAKMLGDHCEKCGAPLFEKDGEVYCPICKIKGNDKKTEDVHKLEEINIEQEEILDDKINYLLERLKDENEISRIIEIGKALEILLRVRESIGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDK0</accession>
    <name>F6BDK0_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 10 12">Glyceraldehyde-3-phosphate dehydrogenase</fullName>
            <shortName evidence="10">GAPDH</shortName>
            <ecNumber evidence="5 10 12">1.2.1.59</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">NAD(P)-dependent glyceraldehyde-3-phosphate dehydrogenase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">gap</name>
        <name evidence="14" type="ordered locus">Metig_1019</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2 10 12">
            <text>D-glyceraldehyde 3-phosphate + NAD(+) + phosphate = (2R)-3-phospho-glyceroyl phosphate + H(+) + NADH</text>
            <dbReference type="Rhea" id="RHEA:10300"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57604"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="1.2.1.59"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 10 12">
            <text>D-glyceraldehyde 3-phosphate + NADP(+) + phosphate = (2R)-3-phospho-glyceroyl phosphate + H(+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:10296"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57604"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="1.2.1.59"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 10 12">Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4 10 12">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10 12">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10 12">Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.59" evidence="5 10 12"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96561.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799163.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1019"/>
    <dbReference type="EnsemblBacteria" id="AEF96561">
        <property type="protein sequence ID" value="AEF96561"/>
        <property type="gene ID" value="Metig_1019"/>
    </dbReference>
    <dbReference type="GeneID" id="10643873"/>
    <dbReference type="KEGG" id="mig:Metig_1019"/>
    <dbReference type="HOGENOM" id="CLU_069533_0_0_2"/>
    <dbReference type="OMA" id="NAIVPNP"/>
    <dbReference type="OrthoDB" id="32079at2157"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00184"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008839">
        <property type="term" value="F:4-hydroxy-tetrahydrodipicolinate reductase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004365">
        <property type="term" value="F:glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047100">
        <property type="term" value="F:glyceraldehyde-3-phosphate dehydrogenase (NADP+) (phosphorylating) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009089">
        <property type="term" value="P:lysine biosynthetic process via diaminopimelate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00559">
        <property type="entry name" value="G3P_dehdrog_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000846">
        <property type="entry name" value="DapB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020831">
        <property type="entry name" value="GlycerAld/Erythrose_P_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020830">
        <property type="entry name" value="GlycerAld_3-P_DH_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020829">
        <property type="entry name" value="GlycerAld_3-P_DH_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020828">
        <property type="entry name" value="GlycerAld_3-P_DH_NAD(P)-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006436">
        <property type="entry name" value="Glyceraldehyde-3-P_DH_2_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01113">
        <property type="entry name" value="DapB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02800">
        <property type="entry name" value="Gp_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000149">
        <property type="entry name" value="GAP_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00846">
        <property type="entry name" value="Gp_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01546">
        <property type="entry name" value="GAPDH-II_archae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00071">
        <property type="entry name" value="GAPDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 12" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 10 12" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="10 12" id="KW-0520">NAD</keyword>
    <keyword evidence="7 10 12" id="KW-0521">NADP</keyword>
    <keyword evidence="8 10 12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gp_dh_N" evidence="13">
        <location>
            <begin position="3"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="region of interest" description="Glyceraldehyde 3-phosphate binding" evidence="10">
        <location>
            <begin position="141"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Glyceraldehyde 3-phosphate binding" evidence="10">
        <location>
            <begin position="196"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="10 11">
        <location>
            <position position="142"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="12"/>
            <end position="13"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="112"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="170"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="304"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00021022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR000149-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="AEF96561.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="340" mass="37638" checksum="71143A388F000FF6" modified="2011-07-27" version="1">MAVKVLINGYGSIGKRVADAVAKQDDMEVIGVTKTKPDFEARLAVEKGYKLFAAIPERKELFEEKGVEIEGTIFDVIEDADIVVDCAPGGIGKDNLENIYKKYKVKAILQGGEKAKYVEDNFNALWSYDRCYGKDYVRVVSCNTTGLCRTLYAIDSVTDIVKARVVLIRRGADPNDAKRGPINAIVPNPPTVPSHHGPDVVSVVPKFEGKILTSAVVVPTTLMHMHTLMVETTGTTRDAILDAIEKTPRIITVKASEGFDSTATIIEYARDLGRMRYDLNEIAIWEESVNVVDNEVFLMQAVHQESDVVPENIDCIRAMLQMEEDNMKSIEKTNKALGIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDL7</accession>
    <name>F6BDL7_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Archaeal glutamate synthase [NADPH]</fullName>
            <ecNumber evidence="4">1.4.1.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Metig_1036</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 4">
            <text>2 L-glutamate + NADP(+) = 2-oxoglutarate + H(+) + L-glutamine + NADPH</text>
            <dbReference type="Rhea" id="RHEA:15501"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="EC" id="1.4.1.13"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the glutamate synthase family.</text>
    </comment>
    <dbReference type="EC" id="1.4.1.13" evidence="4"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96578.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799180.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1036"/>
    <dbReference type="EnsemblBacteria" id="AEF96578">
        <property type="protein sequence ID" value="AEF96578"/>
        <property type="gene ID" value="Metig_1036"/>
    </dbReference>
    <dbReference type="GeneID" id="10643890"/>
    <dbReference type="KEGG" id="mig:Metig_1036"/>
    <dbReference type="HOGENOM" id="CLU_023342_1_1_2"/>
    <dbReference type="OMA" id="CAWGIAT"/>
    <dbReference type="OrthoDB" id="14505at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004355">
        <property type="term" value="F:glutamate synthase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006537">
        <property type="term" value="P:glutamate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02808">
        <property type="entry name" value="GltS_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024188">
        <property type="entry name" value="GltB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043578">
        <property type="entry name" value="GltB_archl_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002932">
        <property type="entry name" value="Glu_synthdom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01645">
        <property type="entry name" value="Glu_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF500061">
        <property type="entry name" value="GOGAT_lg2_archl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006429">
        <property type="entry name" value="GOGAT_lg_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="3 4" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="4" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4" id="KW-0288">FMN</keyword>
    <keyword evidence="4" id="KW-0314">Glutamate biosynthesis</keyword>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0521">NADP</keyword>
    <keyword evidence="4 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="6">
        <location>
            <begin position="10"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="6">
        <location>
            <begin position="39"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="19"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="22"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="25"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="29"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="48"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="51"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="54"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="58"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR006429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR006429-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEF96578.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="510" mass="55499" checksum="8A20D42E89E04BA7" modified="2011-07-27" version="1">MIPSTVPPKFKVEIDHERCMLCERCTIECSWGVLRRHGNRIVAYSNRCGACHRCVSMCPRDAIKIIHQPVSYRPHPLWTEEAREDIYNQAKTGCILLSGMGNAKDFPTYFDKIVLDACQVTNPSIDPLREPMELRTYIGKKPKQLEFEFVEEEIDGKKIKKAKLKTKIAPNLKLDTPIMIAHMSFGALSLNAHKAMAKAVKECGTYMGSGEGGLHRELYPYADHIITQVASGRFGVNEDYLMRGAAIEIKIGQGAKPGIGGHLPGEKVSAEVSMTRMIPEGSDAISPAPHHDIYSIEDLAQLVRSLKEATRWKKPVFVKIAAVHNVAAIAAGIATSDADAVVIDGFKGGTGAAPKVFRDNVGIPIEMAIAAVDERLREEGLRNEISIIASGGIRNSADVFKAIALGADAVYIGTAAMVAMGCRVCGRCYTGLCSWGIATQKPELVQRLDVETAAQRVANLIKAWTHEIKELLGAAGINAIESLRGNRDRLRGVGLNEVELKALGIKHAGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDP3</accession>
    <name>F6BDP3_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein family UPF0150</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_1065</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96604.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799205.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1065"/>
    <dbReference type="EnsemblBacteria" id="AEF96604">
        <property type="protein sequence ID" value="AEF96604"/>
        <property type="gene ID" value="Metig_1065"/>
    </dbReference>
    <dbReference type="GeneID" id="10643919"/>
    <dbReference type="KEGG" id="mig:Metig_1065"/>
    <dbReference type="HOGENOM" id="CLU_2748195_0_0_2"/>
    <dbReference type="OMA" id="EYEVRSH"/>
    <dbReference type="OrthoDB" id="119886at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035157">
        <property type="entry name" value="DUF5395"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035069">
        <property type="entry name" value="TTHA1013/TTHA0281-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17373">
        <property type="entry name" value="DUF5395"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143100">
        <property type="entry name" value="SSF143100"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF96604.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="70" mass="8002" checksum="B803C0E583CBC22B" modified="2011-07-27" version="1">MKTVKFRVYYDGEYWIAEGIDVSIFTQGKTLDELMKNLKEAVELHFEDELKAGEVIKILSVSEMEVSSVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDR2</accession>
    <name>F6BDR2_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Homoserine dehydrogenase</fullName>
            <ecNumber evidence="3">1.1.1.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">Metig_1084</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-homoserine from L-aspartate: step 3/3.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 3/5.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.3" evidence="3"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96623.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799224.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1084"/>
    <dbReference type="EnsemblBacteria" id="AEF96623">
        <property type="protein sequence ID" value="AEF96623"/>
        <property type="gene ID" value="Metig_1084"/>
    </dbReference>
    <dbReference type="GeneID" id="10643938"/>
    <dbReference type="KEGG" id="mig:Metig_1084"/>
    <dbReference type="HOGENOM" id="CLU_009116_1_2_2"/>
    <dbReference type="OMA" id="LMFYGPG"/>
    <dbReference type="OrthoDB" id="102435at2157"/>
    <dbReference type="UniPathway" id="UPA00050">
        <property type="reaction ID" value="UER00063"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00051">
        <property type="reaction ID" value="UER00465"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004412">
        <property type="term" value="F:homoserine dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009088">
        <property type="term" value="P:threonine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005106">
        <property type="entry name" value="Asp/hSer_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001342">
        <property type="entry name" value="HDH_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019811">
        <property type="entry name" value="HDH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022697">
        <property type="entry name" value="HDH_short"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00742">
        <property type="entry name" value="Homoserine_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03447">
        <property type="entry name" value="NAD_binding_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036497">
        <property type="entry name" value="HDH_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01042">
        <property type="entry name" value="HOMOSER_DHGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="5 8" id="KW-0521">NADP</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD_binding_3" evidence="10">
        <location>
            <begin position="7"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="domain" description="Homoserine_dh" evidence="9">
        <location>
            <begin position="154"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="7">
        <location>
            <position position="222"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="7"/>
            <end position="12"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="122"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="207"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR036497-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR036497-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF03447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AEF96623.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="336" mass="36304" checksum="387E885E17363DF1" modified="2011-07-27" version="1">MKIILVGFGVIGRGVAKVIDEKKKLLKEKYGFEPKIVAICDSSGAAIDESGLDLKKAIEVKEKTGKIANYNGKKISSVEVIESIDADVVVEATPTNIKTGEPARTHILKAFENGKHVVTANKGPLALYFKELVESAEKHGVMFRYEASVGGAMPIINLAKETLAGNEIKSIKGILNGTTNYILTKMEKEKLDFNTVLKEAQELGIAETDPSQDIYGYDTAAKIVILANSIMGMNKTIDDVKIEGITRITPEAIELANKSGYTIKLIGEVKKNKLVVEPRLVQLDSPLNVKGTLNVAMFNTDLAKEVVIVGRGAGATETASAILSDLINIHLKVKEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDT0</accession>
    <name>F6BDT0_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Probable manganese-dependent inorganic pyrophosphatase</fullName>
            <ecNumber evidence="8">3.6.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Pyrophosphate phospho-hydrolase</fullName>
            <shortName evidence="8">PPase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">ppaC</name>
        <name evidence="10" type="ordered locus">Metig_1102</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>diphosphate + H2O = H(+) + 2 phosphate</text>
            <dbReference type="Rhea" id="RHEA:24576"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="EC" id="3.6.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="8">Binds 2 manganese ions per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the PPase class C family.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.1" evidence="8"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96641.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799242.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1102"/>
    <dbReference type="EnsemblBacteria" id="AEF96641">
        <property type="protein sequence ID" value="AEF96641"/>
        <property type="gene ID" value="Metig_1102"/>
    </dbReference>
    <dbReference type="GeneID" id="10643960"/>
    <dbReference type="KEGG" id="mig:Metig_1102"/>
    <dbReference type="HOGENOM" id="CLU_025243_0_1_2"/>
    <dbReference type="OMA" id="IMLCAIL"/>
    <dbReference type="OrthoDB" id="57518at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004427">
        <property type="term" value="F:inorganic diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.310.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00207">
        <property type="entry name" value="PPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001667">
        <property type="entry name" value="DDH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038763">
        <property type="entry name" value="DHH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004097">
        <property type="entry name" value="DHHA2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038222">
        <property type="entry name" value="DHHA2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022934">
        <property type="entry name" value="Mn-dep_inorganic_PyrPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01368">
        <property type="entry name" value="DHH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02833">
        <property type="entry name" value="DHHA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01131">
        <property type="entry name" value="DHHA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64182">
        <property type="entry name" value="SSF64182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 8 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 8" id="KW-0464">Manganese</keyword>
    <keyword evidence="5 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DHHA2" evidence="9">
        <location>
            <begin position="180"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="7"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="11"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="13"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="66"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="88"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="147"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM01131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AEF96641.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="307" mass="34047" checksum="64C694EF21F896CC" modified="2011-07-27" version="1">MLYVVGHKNPDTDSICSAIVLAHFLDAYPARQGDLNPETEFVLRKFGVMEPELITSAEGKEIILVDHSEKSQSLDDLEKGKLVGIVDHHKIGITTSEPILYYAKPVGSTATVIAELYFKNAMDLIGGKNKELTPDLAGLLLSAIISDTVLFKSPTTTDLDKEMAKKLAEIANIEDINAFGMEILKAKSVVGKMKPEEIITMDYKDFEFNGKKVGIGQVEVIDVSEVEDKKEEIYKLLEEKLKNEGYDLIVFLITDIMKEGSEALVVGNKEVFEKAFNVKVEGNSVFLEGVMSRKKQVVPPLEKAYNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDU2</accession>
    <name>F6BDU2_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">UBA/THIF-type NAD/FAD binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_1114</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96653.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799254.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1114"/>
    <dbReference type="EnsemblBacteria" id="AEF96653">
        <property type="protein sequence ID" value="AEF96653"/>
        <property type="gene ID" value="Metig_1114"/>
    </dbReference>
    <dbReference type="GeneID" id="10643972"/>
    <dbReference type="KEGG" id="mig:Metig_1114"/>
    <dbReference type="HOGENOM" id="CLU_1346434_0_0_2"/>
    <dbReference type="OMA" id="DMEPITP"/>
    <dbReference type="OrthoDB" id="78693at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008641">
        <property type="term" value="F:ubiquitin-like modifier activating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035985">
        <property type="entry name" value="Ubiquitin-activating_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012028">
        <property type="entry name" value="UCP006529_dinclt"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006529">
        <property type="entry name" value="UCP006529_dinclt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69572">
        <property type="entry name" value="SSF69572"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF96653.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="203" mass="22475" checksum="BB4A399C6B0B6588" modified="2011-07-27" version="1">MDNIAELEKKLTRKGEVSIVGCGRLGVRVAFDLLEVHRGGPKKVYLFDNAKIAENDIVHRRLGGKIGEYKVKFVERFFGDRVVGITENINEDNLHLLTGDVVIICIAGGDTIPTTRAIIDYAKKRGIKTIGTNGVFGIEENIKVCDAKYAKGPAQFLNLDKEGHIVVGTGKFIRDLEPITPYTLDEIAKKIVIECLKVLNKIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDX6</accession>
    <name>F6BDX6_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="4">TrkA-N domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metig_1148</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96687.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799288.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1148"/>
    <dbReference type="EnsemblBacteria" id="AEF96687">
        <property type="protein sequence ID" value="AEF96687"/>
        <property type="gene ID" value="Metig_1148"/>
    </dbReference>
    <dbReference type="GeneID" id="10644006"/>
    <dbReference type="KEGG" id="mig:Metig_1148"/>
    <dbReference type="HOGENOM" id="CLU_050982_0_1_2"/>
    <dbReference type="OMA" id="VFEGIWW"/>
    <dbReference type="OrthoDB" id="80000at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008324">
        <property type="term" value="F:cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006813">
        <property type="term" value="P:potassium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013099">
        <property type="entry name" value="K_chnl_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006037">
        <property type="entry name" value="RCK_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036721">
        <property type="entry name" value="RCK_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003148">
        <property type="entry name" value="RCK_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07885">
        <property type="entry name" value="Ion_trans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02080">
        <property type="entry name" value="TrkA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02254">
        <property type="entry name" value="TrkA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF116726">
        <property type="entry name" value="SSF116726"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51202">
        <property type="entry name" value="RCK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51201">
        <property type="entry name" value="RCK_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="33"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="62"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="domain" description="RCK N-terminal" evidence="2">
        <location>
            <begin position="109"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="domain" description="RCK C-terminal" evidence="3">
        <location>
            <begin position="248"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEF96687.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="339" mass="37312" checksum="828FAB9BE0E5B97C" modified="2011-07-27" version="1">MDTLNKIKLGMVTIGALILFASIGFMFLEGWDFFTALYVSIVTISTVGYGDYTPTTHWGKALVMVYIVTGVGAVAYTFGSIAEFFMEGHFKKVARMRKMEKNLKKLKGHFIICGYGKLGKVVAEKFKNAKVPFVVIDSDEKMLEEEAEKDPDLIYVVGDATSDDVLIKAGIERARGLISVVNTNAENVFITLSAKRLNPNIYVVAKAEDGSAVDKLLKAGADRVVSPNVIGGSRIAEIALKPEVLDFVSTIMDDSYDMEIEKFCVCEGSEICGKSLYESQIRSKTGVTILAVKKGNDLIINPSPDVVVDKGDEIYAFGTKEQLEKLEKILNKTKKRKNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2020-12-02" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDY4</accession>
    <name>F6BDY4_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Metig_1156</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96695.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96695">
        <property type="protein sequence ID" value="AEF96695"/>
        <property type="gene ID" value="Metig_1156"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_1156"/>
    <dbReference type="HOGENOM" id="CLU_1840580_0_0_2"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006603">
        <property type="entry name" value="PQ-loop_rpt"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04193">
        <property type="entry name" value="PQ-loop"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="50"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="80"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="106"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEF96695.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="139" mass="16234" checksum="31A1014EC4111017" modified="2011-07-27" version="1">MSLKINDKTSGFAIYNSAHNYKYHKLFIVGMQNYVKKNFWDGFMAIEFDMVGYIAGTLTTFASLPQLIKSLKTKDMSGISLYFVVTFTLGLSLWLVYGILKNDYPIIIFNIISLMFWIPITYLKVKDELRRKLNYSRVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDZ6</accession>
    <name>F6BDZ6_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Glycosyl transferase family 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_1169</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96707.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1169"/>
    <dbReference type="EnsemblBacteria" id="AEF96707">
        <property type="protein sequence ID" value="AEF96707"/>
        <property type="gene ID" value="Metig_1169"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_1169"/>
    <dbReference type="HOGENOM" id="CLU_033536_7_4_2"/>
    <dbReference type="OMA" id="DGDYQHK"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="128"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="domain" description="Glyco_trans_2-like" evidence="2">
        <location>
            <begin position="3"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF96707.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="256" mass="29697" checksum="04CF8F7E869325F3" modified="2011-07-27" version="1">MIAVIPMFNEERNILKVLRDLEELGIDAVVVDDGSKDNSSKIVEEFSKKSKNRIYLIKKEKNEGKARALKDGTEFALKLNPDYIIYMDGDYQHKPKDIPKMIEKLKKENADAVFGIRKYKHIPFHRQISNFLASLIMSLVVTFYSGKIYIFRDIQCGYRIIKAKFLKDVYFGEGYSVEHLIALQLAKNRAKIVEEYVDIEYHPDATSYITTKKILDVAKEVAKFVLKGLPKNESKTWILLCFFHKIHQIIKSITLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>F6BE22</accession>
    <name>F6BE22_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CoB--CoM heterodisulfide reductase iron-sulfur subunit A</fullName>
            <ecNumber evidence="4">1.8.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Metig_1195</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Part of a complex that catalyzes the reversible reduction of CoM-S-S-CoB to the thiol-coenzymes H-S-CoM (coenzyme M) and H-S-CoB (coenzyme B).</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 4">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4">Cofactor metabolism; coenzyme M-coenzyme B heterodisulfide reduction; coenzyme B and coenzyme M from coenzyme M-coenzyme B heterodisulfide: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">The ferredoxin:CoB-CoM heterodisulfide reductase is composed of three subunits; HdrA, HdrB and HdrC.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the HdrA family.</text>
    </comment>
    <dbReference type="EC" id="1.8.-.-" evidence="4"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96733.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799332.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1195"/>
    <dbReference type="EnsemblBacteria" id="AEF96733">
        <property type="protein sequence ID" value="AEF96733"/>
        <property type="gene ID" value="Metig_1195"/>
    </dbReference>
    <dbReference type="GeneID" id="10644055"/>
    <dbReference type="KEGG" id="mig:Metig_1195"/>
    <dbReference type="HOGENOM" id="CLU_020302_0_0_2"/>
    <dbReference type="OMA" id="TAKHAML"/>
    <dbReference type="OrthoDB" id="1148at2157"/>
    <dbReference type="UniPathway" id="UPA00647">
        <property type="reaction ID" value="UER00700"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039650">
        <property type="entry name" value="HdrA-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43498">
        <property type="entry name" value="PTHR43498"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12838">
        <property type="entry name" value="Fer4_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13187">
        <property type="entry name" value="Fer4_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="3 4" id="KW-0274">FAD</keyword>
    <keyword evidence="4" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="5">
        <location>
            <begin position="236"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="5">
        <location>
            <begin position="284"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="5">
        <location>
            <begin position="575"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="5">
        <location>
            <begin position="608"/>
            <end position="637"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU366072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEF96733.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="654" mass="71627" checksum="5AEBC628C607D1E8" modified="2011-07-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>F6BE28</accession>
    <name>F6BE28_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 5 6">DNA repair and recombination protein RadA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">radA</name>
        <name evidence="9" type="ordered locus">Metig_0002</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5 6">Involved in DNA repair and in homologous recombination. Binds and assemble on single-stranded DNA to form a nucleoprotein filament. Hydrolyzes ATP in a ssDNA-dependent manner and promotes DNA strand exchange between homologous DNA molecules.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5 6">Belongs to the eukaryotic RecA-like protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95564.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798174.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0002"/>
    <dbReference type="EnsemblBacteria" id="AEF95564">
        <property type="protein sequence ID" value="AEF95564"/>
        <property type="gene ID" value="Metig_0002"/>
    </dbReference>
    <dbReference type="GeneID" id="10642837"/>
    <dbReference type="KEGG" id="mig:Metig_0002"/>
    <dbReference type="HOGENOM" id="CLU_041732_0_0_2"/>
    <dbReference type="OMA" id="TFRIYLR"/>
    <dbReference type="OrthoDB" id="30130at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01123">
        <property type="entry name" value="Rad51_DMC1_radA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00348">
        <property type="entry name" value="RadA_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013632">
        <property type="entry name" value="DNA_recomb/repair_Rad51_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011938">
        <property type="entry name" value="DNA_recomb/repair_RadA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016467">
        <property type="entry name" value="DNA_recomb/repair_RecA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010995">
        <property type="entry name" value="DNA_repair_Rad51/TF_NusA_a-hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003583">
        <property type="entry name" value="Hlx-hairpin-Hlx_DNA-bd_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033925">
        <property type="entry name" value="Rad51_DMC1_RadA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020588">
        <property type="entry name" value="RecA_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020587">
        <property type="entry name" value="RecA_monomer-monomer_interface"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08423">
        <property type="entry name" value="Rad51"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005856">
        <property type="entry name" value="Rad51"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00278">
        <property type="entry name" value="HhH1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47794">
        <property type="entry name" value="SSF47794"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02236">
        <property type="entry name" value="recomb_radA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50162">
        <property type="entry name" value="RECA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50163">
        <property type="entry name" value="RECA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 6" id="KW-0227">DNA damage</keyword>
    <keyword evidence="5 6" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="5 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RECA_2" evidence="7">
        <location>
            <begin position="76"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="domain" description="RECA_3" evidence="8">
        <location>
            <begin position="263"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="105"/>
            <end position="112"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00018144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00348"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR005856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AEF95564.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="322" mass="35437" checksum="2FFFF13A517E13E3" modified="2011-07-27" version="1">MADDLTKLPGVGQSIAEKLIEAGYIDYMKIATATIGELSEIDGISEKAAAKIIAAAREYCNIGFKSGADLLNQRKTVWKLSTGSKELDNILDGGLESQSVTEFAGMFGSGKTQIMHQACVNLQCEDRIIADDSIKDEILEKPLKAVYIDTEGTFRPERIMQMAEALGIDGKDVLDNTFVARAYNSDMQMLFAEKIEDLIKEGHNIKLVVVDSLTSTFRNEYTGRGKLAERQQKLGRHMATLNKLADLYNCVVIVTNQVAARPDAFFGSSEQAIGGHIVGHAATFRIFLRKAKGDKRVAKLYDSPHLPDAEAMFRITEKGIHD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>F6BE34</accession>
    <name>F6BE34_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PrcB_C domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0008</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95570.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798180.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0008"/>
    <dbReference type="EnsemblBacteria" id="AEF95570">
        <property type="protein sequence ID" value="AEF95570"/>
        <property type="gene ID" value="Metig_0008"/>
    </dbReference>
    <dbReference type="GeneID" id="10642843"/>
    <dbReference type="KEGG" id="mig:Metig_0008"/>
    <dbReference type="HOGENOM" id="CLU_138329_0_0_2"/>
    <dbReference type="OMA" id="VYPYEIV"/>
    <dbReference type="OrthoDB" id="111220at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025748">
        <property type="entry name" value="PrcB_C_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14343">
        <property type="entry name" value="PrcB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PrcB_C" evidence="1">
        <location>
            <begin position="87"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF14343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF95570.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="157" mass="17963" checksum="0B24E4F8B23507EC" modified="2011-07-27" version="1">MKRLFALVLAIISILTILLCGCIENETIDQDSNSNLSDMINNTNKINLNETKNLQENITIPFEIIEMGMFGKEKSRRFIHYIKNNKTVIEVYLGEKPNTGYSISITKIIKHNDKLIVYVNESYREGVHAMVITSPYEIVEVNGTYDNVVFELIGKDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>F6BE60</accession>
    <name>F6BE60_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 9">Phosphoribosylamine--glycine ligase</fullName>
            <ecNumber evidence="4 9">6.3.4.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">GARS</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Glycinamide ribonucleotide synthetase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Phosphoribosylglycinamide synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">purD</name>
        <name evidence="12" type="ordered locus">Metig_0035</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>5-phospho-beta-D-ribosylamine + ATP + glycine = ADP + H(+) + N(1)-(5-phospho-beta-D-ribosyl)glycinamide + phosphate</text>
            <dbReference type="Rhea" id="RHEA:17453"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:58681"/>
            <dbReference type="ChEBI" id="CHEBI:143788"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.4.13"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 9">Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the GARS family.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.13" evidence="4 9"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95596.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798205.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0035"/>
    <dbReference type="EnsemblBacteria" id="AEF95596">
        <property type="protein sequence ID" value="AEF95596"/>
        <property type="gene ID" value="Metig_0035"/>
    </dbReference>
    <dbReference type="GeneID" id="10642870"/>
    <dbReference type="KEGG" id="mig:Metig_0035"/>
    <dbReference type="HOGENOM" id="CLU_027420_3_0_2"/>
    <dbReference type="OMA" id="KATVCKY"/>
    <dbReference type="OrthoDB" id="58022at2157"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00125"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004637">
        <property type="term" value="F:phosphoribosylamine-glycine ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009113">
        <property type="term" value="P:purine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.600.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00138">
        <property type="entry name" value="GARS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020561">
        <property type="entry name" value="PRibGlycinamid_synth_ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000115">
        <property type="entry name" value="PRibGlycinamide_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020560">
        <property type="entry name" value="PRibGlycinamide_synth_C-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037123">
        <property type="entry name" value="PRibGlycinamide_synth_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020559">
        <property type="entry name" value="PRibGlycinamide_synth_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020562">
        <property type="entry name" value="PRibGlycinamide_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011054">
        <property type="entry name" value="Rudment_hybrid_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43472">
        <property type="entry name" value="PTHR43472"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01071">
        <property type="entry name" value="GARS_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02843">
        <property type="entry name" value="GARS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02844">
        <property type="entry name" value="GARS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01210">
        <property type="entry name" value="GARS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51246">
        <property type="entry name" value="SSF51246"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="SSF52440"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00877">
        <property type="entry name" value="purD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00184">
        <property type="entry name" value="GARS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 9 12" id="KW-0436">Ligase</keyword>
    <keyword evidence="6 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 9" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-grasp" evidence="11">
        <location>
            <begin position="109"/>
            <end position="324"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AEF95596.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="446" mass="49320" checksum="5304034ADFA3A08D" modified="2011-07-27" version="1">MKILLIGGGARESAIAHALKKNADVKLYTLMNNKNPGIARLSEKISLNKETDLDAVKAFAEEVKPDMAVIGPEAPLGAGVADLLWDMGIPTVGPKKLPAQIETSKEFMRNLFKKYNIKGSLQYKAFEEYGEELEAFIDEMTNQGKDVVVKPVGLTGGKGVKVVGEQLKDNEEAKQYAKEVFEKNIGGGKVIIEERLIGVEFTLHGFVDGDTIKFTPAVQDHPHAYENDEGPITGGMGSYSCPDLSLPFLTENDLKDAKEIMEDTVKALKKEVGDYQGFLYGQFMLTADGPKIIEYNARFGDPEAMNILAILKNDFVEVCEAIANKKLKDIDVKFENKATVCKYVVPNGYPTNPVRNKEIFVDEEKIKETGAILHYASVNEVDGKLYMTGSRSVAVVGVADTIEEAEKIAESAIQYIKGDVFHRSDVGKLDLIKKRIERMKELRENK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>F6BE71</accession>
    <name>F6BE71_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="3">2-hydroxyglutaryl-CoA dehydratase D-component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_1210</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FldB/FldC dehydratase alpha/beta subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96748.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1210"/>
    <dbReference type="EnsemblBacteria" id="AEF96748">
        <property type="protein sequence ID" value="AEF96748"/>
        <property type="gene ID" value="Metig_1210"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_1210"/>
    <dbReference type="HOGENOM" id="CLU_053697_1_1_2"/>
    <dbReference type="OMA" id="RICPYFQ"/>
    <dbReference type="OrthoDB" id="34491at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010327">
        <property type="entry name" value="FldB/FldC_alpha/beta"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06050">
        <property type="entry name" value="HGD-D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="202"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF96748.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="372" mass="42702" checksum="836833F287A09F6E" modified="2011-07-27" version="1">MELNAIKKLMEKFANRKEQLYKQKEEGKKVFGMFCAFVPIELILAADAIPVGLCGGKNDTVPIAEEDLPRNLCPLIKSSYGFKKAKSCPYFEAADVVIGETTCDGKKKMFELLERMVKMHVMQLPYMKTEKAKELWVEEVKKLKEMIEKETGNKIDEEKLKESVEKVNKMRKLFYKLYELRANKPTPIKGIDALKLFQFAYLLDIDDTIEILEELVKELEEKVKKGEGYEGKRILVSGCPMVAGNTKIVELIEEVGGVVVGEESCTGTRFFENLVEEPTIEGIAERYLKIPCACMFHNDERIENIKRLVKELNADGVVYYTLQYCHTFNVEGARVEEELKKEGIPVIRIETDYSESDKEQLKTRLEAFIEMI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEE3</accession>
    <name>F6BEE3_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Selenide, water dikinase</fullName>
            <ecNumber evidence="5">2.7.9.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Selenium donor protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">Selenophosphate synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">selD</name>
        <name evidence="8" type="ordered locus">Metig_0043</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Synthesizes selenophosphate from selenide and ATP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>ATP + H2O + hydrogenselenide = AMP + 2 H(+) + phosphate + selenophosphate</text>
            <dbReference type="Rhea" id="RHEA:18737"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16144"/>
            <dbReference type="ChEBI" id="CHEBI:29317"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.7.9.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="5">Binds 1 Mg(2+) ion per monomer.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the selenophosphate synthase 1 family. Class I subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.9.3" evidence="5"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95604.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0043"/>
    <dbReference type="KEGG" id="mig:Metig_0043"/>
    <dbReference type="HOGENOM" id="CLU_032859_1_0_2"/>
    <dbReference type="OMA" id="DNEPKYG"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004756">
        <property type="term" value="F:selenide, water dikinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016260">
        <property type="term" value="P:selenocysteine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02195">
        <property type="entry name" value="SelD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.650.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00625">
        <property type="entry name" value="SelD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010918">
        <property type="entry name" value="PurM-like_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036676">
        <property type="entry name" value="PurM-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016188">
        <property type="entry name" value="PurM-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036921">
        <property type="entry name" value="PurM-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023061">
        <property type="entry name" value="SelD_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004536">
        <property type="entry name" value="SPS/SelD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10256">
        <property type="entry name" value="PTHR10256"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00586">
        <property type="entry name" value="AIRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02769">
        <property type="entry name" value="AIRS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036407">
        <property type="entry name" value="Selenphspht_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55326">
        <property type="entry name" value="SSF55326"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56042">
        <property type="entry name" value="SSF56042"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00476">
        <property type="entry name" value="selD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 5 8" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5 8" id="KW-0711">Selenium</keyword>
    <keyword evidence="8" id="KW-0712">Selenocysteine</keyword>
    <keyword evidence="2 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="AIRS" evidence="6">
        <location>
            <begin position="51"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="domain" description="AIRS_C" evidence="7">
        <location>
            <begin position="169"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="17"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="20"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="49"/>
            <end position="51"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="52"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="68"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="91"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="139"/>
            <end position="141"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="245"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="site" description="Important for catalytic activity" evidence="5">
        <location>
            <position position="20"/>
        </location>
    </feature>
    <feature type="non-standard amino acid" description="Selenocysteine" evidence="8">
        <location>
            <position position="17"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00625"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEF95604.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="348" mass="37677" checksum="5FC1635F121EEBB4" modified="2011-07-27" version="1">MEEKKVRLTEMVKLHGUACKLPSTDLENLVKGIVSNEEITKSKDIIVGLGDDAAVVKRNGLVIAKTVDVFTPIVDDPYLQGKIAACNSTSDIYAMGLLDIVGVLVILGIPENLPIDAAKEMLRGFQDFCRENDTSIIGGHTIMNPWPLIGGAVTGVGREEDVLTNAGAKVGDTLILTKPLGTQTAMALSRVPKEFKDMLGVSEEEEEYIINKAIELMTTSNRTALVSLRNAEKKVGEKIANALTDITGFGILGHANEVARNSRVIIEINKLPTIKKTPELSRMFGHPLLEGCGAETAGGLLISVKEEYKEELIDELTKNGCYAFEVGKVIAKDREGKACLKNPEIVEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEF5</accession>
    <name>F6BEF5_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">EamA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_0055</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95616.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0055"/>
    <dbReference type="EnsemblBacteria" id="AEF95616">
        <property type="protein sequence ID" value="AEF95616"/>
        <property type="gene ID" value="Metig_0055"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0055"/>
    <dbReference type="HOGENOM" id="CLU_120467_2_1_2"/>
    <dbReference type="OMA" id="WGIAPIF"/>
    <dbReference type="OrthoDB" id="127631at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000620">
        <property type="entry name" value="EamA_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00892">
        <property type="entry name" value="EamA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="36"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="68"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="99"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="124"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="2">
        <location>
            <begin position="5"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF95616.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="142" mass="15604" checksum="048315548E1B1D5C" modified="2011-07-27" version="1">MGDKMKGEFFALLAALCWGLCPIIEKKALELTDSSTAIFIKSLMDFIIISLIFASTGKIMTMKIDYRVLIYLTLVSLIAGITGQYFFYKGLSMSSPSKVVVISSIYPFVTIMLSTLILGKMPSVKVWIGAILIFIGVSLVVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEG3</accession>
    <name>F6BEG3_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Carbamoyl-phosphate synthase (Glutamine-hydrolyzing)</fullName>
            <ecNumber evidence="7">6.3.5.5</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Metig_0063</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-arginine biosynthesis; carbamoyl phosphate from bicarbonate: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.5" evidence="7"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95624.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0063"/>
    <dbReference type="EnsemblBacteria" id="AEF95624">
        <property type="protein sequence ID" value="AEF95624"/>
        <property type="gene ID" value="Metig_0063"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0063"/>
    <dbReference type="HOGENOM" id="CLU_000513_1_1_2"/>
    <dbReference type="OMA" id="ADRMFYL"/>
    <dbReference type="UniPathway" id="UPA00068">
        <property type="reaction ID" value="UER00171"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006526">
        <property type="term" value="P:arginine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1030.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005480">
        <property type="entry name" value="CarbamoylP_synth_lsu_oligo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036897">
        <property type="entry name" value="CarbamoylP_synth_lsu_oligo_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005479">
        <property type="entry name" value="CbamoylP_synth_lsu-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005483">
        <property type="entry name" value="CbamoylP_synth_lsu_CPSase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02786">
        <property type="entry name" value="CPSase_L_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02787">
        <property type="entry name" value="CPSase_L_D3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00098">
        <property type="entry name" value="CPSASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01096">
        <property type="entry name" value="CPSase_L_D3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48108">
        <property type="entry name" value="SSF48108"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="SSF52440"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00866">
        <property type="entry name" value="CPSASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00867">
        <property type="entry name" value="CPSASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="3" id="KW-0055">Arginine biosynthesis</keyword>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0436">Ligase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-grasp" evidence="6">
        <location>
            <begin position="132"/>
            <end position="326"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEF95624.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="482" mass="53774" checksum="2AFFBA0248358429" modified="2011-07-27" version="1">MKREDINKVMILGSGPIVIGQAAEFDFSGSQACKALREEGVYTILVNSNPATIQTDTNIADKVYLEPLHPKIVEKIIEKERPDAILPTMGGQTGLNLAMELYKRGVLDKYGVELIGSNARVIEICEDRALFDKAMKEIGEPTTKSKAVNSVEEALEFVEEIGYPVIVRPAFTLGGTGGGIAHNEEELIEIVKKGLKYSIINQVLIDESVLGWKEFEYEVMRDRKNTCIIVCNMENIDPMGIHTGESIVVAPAQTLSDEFHQKLRSAALKIIRHLGIEGGCNIQFAVNKEMTEYRVIEVNPRVSRSSALASKATGYPIARIAAKIAIGMTLDEIRNDVTKETPASFEPTLDYVVVKIPRWPFDKFRTVDKRLGTSMKSTGEVMAIGRSFEEALQKAIRSLDIGRFGLIGDGKDKEYTDEEIEDILKNPTDERIFVIAYALDRGWSVEKIAELTNIDEFFIRKIKNIVDMKKELEKLGKELKGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEK9</accession>
    <name>F6BEK9_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Transcriptional regulator, AsnC family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Metig_0109</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95670.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0109"/>
    <dbReference type="EnsemblBacteria" id="AEF95670">
        <property type="protein sequence ID" value="AEF95670"/>
        <property type="gene ID" value="Metig_0109"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0109"/>
    <dbReference type="HOGENOM" id="CLU_091233_5_4_2"/>
    <dbReference type="OMA" id="EDCWFIA"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000485">
        <property type="entry name" value="AsnC-type_HTH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019888">
        <property type="entry name" value="Tscrpt_reg_AsnC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019887">
        <property type="entry name" value="Tscrpt_reg_AsnC/Lrp_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019885">
        <property type="entry name" value="Tscrpt_reg_HTH_AsnC-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01037">
        <property type="entry name" value="AsnC_trans_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13404">
        <property type="entry name" value="HTH_AsnC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00033">
        <property type="entry name" value="HTHASNC"/>
    </dbReference>
    <dbReference type="SMART" id="SM00344">
        <property type="entry name" value="HTH_ASNC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="SSF54909"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00519">
        <property type="entry name" value="HTH_ASNC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50956">
        <property type="entry name" value="HTH_ASNC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH asnC-type" evidence="4">
        <location>
            <begin position="2"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEF95670.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="141" mass="16009" checksum="126BEA6BFCDEB41F" modified="2011-07-27" version="1">MLDAKDMKILEILMKDGRKPFTEIAKELNTSESSIRKRVRKMEKEGIIKGYIAVIDPSKIGYNVVALTGFDTDPQNFLMVAKKLCEFEEVKKVYTSTGDHMIMAEIWAKDGQELTNIIFNKIGKIEGIKKVCPAIILEQLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-06-02" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEN2</accession>
    <name>F6BEN2_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_1292</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96829.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799426.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96829">
        <property type="protein sequence ID" value="AEF96829"/>
        <property type="gene ID" value="Metig_1292"/>
    </dbReference>
    <dbReference type="GeneID" id="10644155"/>
    <dbReference type="KEGG" id="mig:Metig_1292"/>
    <dbReference type="HOGENOM" id="CLU_1860681_0_0_2"/>
    <dbReference type="OMA" id="KWFVVKK"/>
    <dbReference type="OrthoDB" id="94150at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022620">
        <property type="entry name" value="DUF2666"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10869">
        <property type="entry name" value="DUF2666"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF96829.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="139" mass="16303" checksum="7F3C20262C724D68" modified="2011-07-27" version="1">MENRIQFIAKHNDWFVVKKMNIDDKTENIEIARLLSSIDETVLNKLPEYLPFDMKKLEEIADEIYQKKKGRVKEEDVVNALMKLKSPSTTKKLGEITDSKEGKEILKVILNNIILERLGIKTRVDPKLIEKYIEKMNEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEN5</accession>
    <name>F6BEN5_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Universal PUA-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1295</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96832.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799429.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1295"/>
    <dbReference type="EnsemblBacteria" id="AEF96832">
        <property type="protein sequence ID" value="AEF96832"/>
        <property type="gene ID" value="Metig_1295"/>
    </dbReference>
    <dbReference type="GeneID" id="10644158"/>
    <dbReference type="KEGG" id="mig:Metig_1295"/>
    <dbReference type="HOGENOM" id="CLU_090468_1_1_2"/>
    <dbReference type="OMA" id="HDHIEIL"/>
    <dbReference type="OrthoDB" id="85279at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.130.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022430">
        <property type="entry name" value="CHP03684"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015266">
        <property type="entry name" value="DUF1947"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016437">
        <property type="entry name" value="MCT-1/Tma20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002478">
        <property type="entry name" value="PUA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036974">
        <property type="entry name" value="PUA_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004521">
        <property type="entry name" value="Uncharacterised_CHP00451"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22798">
        <property type="entry name" value="PTHR22798"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09183">
        <property type="entry name" value="DUF1947"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01472">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005067">
        <property type="entry name" value="Tma_RNA-bind_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00359">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="SSF88697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03684">
        <property type="entry name" value="arCOG00985"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00451">
        <property type="entry name" value="unchar_dom_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PUA" evidence="1">
        <location>
            <begin position="79"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96832.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="160" mass="18201" checksum="1460CD468EE279E0" modified="2011-07-27" version="1">MEIKRRYHLKKKELKQIKEELSKFFTDIDDIIPKKANVEIAITDKYDMILVDNKPVAVRLNNKLYPTLKVLLDKDIKEKRVVVDMGAVKFLANGADVMAPGIVDADENIKEGDVVFVVDEKHNKPLCVGEALMDGKTMKEANKGRAIKTVHHVGDDIWKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-06-02" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEP3</accession>
    <name>F6BEP3_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_1303</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96840.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799437.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96840">
        <property type="protein sequence ID" value="AEF96840"/>
        <property type="gene ID" value="Metig_1303"/>
    </dbReference>
    <dbReference type="GeneID" id="10644166"/>
    <dbReference type="KEGG" id="mig:Metig_1303"/>
    <dbReference type="HOGENOM" id="CLU_445940_0_0_2"/>
    <dbReference type="OMA" id="VVWANCT"/>
    <dbReference type="OrthoDB" id="16428at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF96840.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="619" mass="67701" checksum="B2B2955876B407BB" modified="2011-07-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>F6BER8</accession>
    <name>F6BER8_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">GTPase HflX</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">GTP-binding protein HflX</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">hflX</name>
        <name evidence="10" type="ordered locus">Metig_1328</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">GTPase that associates with the 50S ribosomal subunit and may have a role during protein synthesis or ribosome biogenesis.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="5">Monomer. Associates with the 50S ribosomal subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
        <text evidence="5">May associate with membranes.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the TRAFAC class OBG-HflX-like GTPase superfamily. HflX GTPase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96865.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1328"/>
    <dbReference type="EnsemblBacteria" id="AEF96865">
        <property type="protein sequence ID" value="AEF96865"/>
        <property type="gene ID" value="Metig_1328"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_1328"/>
    <dbReference type="HOGENOM" id="CLU_019597_2_0_2"/>
    <dbReference type="OMA" id="FNNHAHV"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11060">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00900">
        <property type="entry name" value="GTPase_HflX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030394">
        <property type="entry name" value="G_HFLX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032305">
        <property type="entry name" value="GTP-bd_M"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016496">
        <property type="entry name" value="GTPase_HflX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025121">
        <property type="entry name" value="GTPase_HflX_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042108">
        <property type="entry name" value="GTPase_HflX_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10229">
        <property type="entry name" value="PTHR10229"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16360">
        <property type="entry name" value="GTP-bdg_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13167">
        <property type="entry name" value="GTP-bdg_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006809">
        <property type="entry name" value="GTP-binding_hflX_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03156">
        <property type="entry name" value="GTP_HflX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51705">
        <property type="entry name" value="G_HFLX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 5 6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3 7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="1 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 5 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Hflx-type G" evidence="9">
        <location>
            <begin position="189"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="157"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="195"/>
            <end position="202"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="202"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="220"/>
            <end position="224"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="222"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="241"/>
            <end position="244"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="308"/>
            <end position="311"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="336"/>
            <end position="338"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR006809-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR006809-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AEF96865.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="411" mass="47292" checksum="4C29F2D2D62ADAB6" modified="2011-07-27" version="1">MINVLRKRAMLILRKEFSERKNIEELKELASVLYEPVECVVQVRPPHPKYQIGSGLVEKLVEKIKEKDIEIVIFDNRLSPTQKYNLAGKFGVEVIDKVELVLRIFYKHAHTKEAQLQVRLAELQYELPQAKEKVRLAKLGERPGFGGYGDYEVNKYYNKIKREIAIIKEKLKKIREHRKRLRKKRKKFEVVGLIGYTNAGKTSLLNALTGENKTSKNQVFTTLTTTTRAIKNAPRKILVTDTVGFMEDLPPFMIEAFLSTIEESAESDLILIVVDASDSIEDIERKLKTNYEILSKIGCSAPIITVFNKCDKIDEEKKKEILTSLERYIVSPIFVSAKYHINIDKLIELILEKLNASIGVVETSDMKLISYLYENTEIIEKIEENGKYIVTFRAKEEEVNRILRIANKLGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>F6BET2</accession>
    <name>F6BET2_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CRISPR system Cms protein Csm5</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">CRISPR type III A-associated protein Csm5</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Metig_1344</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">This subunit might be involved in maturation of a crRNA intermediate to its mature form.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CRISPR-associated Csm5 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96879.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799476.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1344"/>
    <dbReference type="EnsemblBacteria" id="AEF96879">
        <property type="protein sequence ID" value="AEF96879"/>
        <property type="gene ID" value="Metig_1344"/>
    </dbReference>
    <dbReference type="GeneID" id="10644207"/>
    <dbReference type="KEGG" id="mig:Metig_1344"/>
    <dbReference type="HOGENOM" id="CLU_036878_3_0_2"/>
    <dbReference type="OMA" id="KSAGRFY"/>
    <dbReference type="OrthoDB" id="41800at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010173">
        <property type="entry name" value="CRISPR-assoc_Csm5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005537">
        <property type="entry name" value="RAMP_III_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38007">
        <property type="entry name" value="PTHR38007"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03787">
        <property type="entry name" value="RAMPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01899">
        <property type="entry name" value="cas_TM1807_csm5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0051">Antiviral defense</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0694">RNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00031720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEF96879.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="364" mass="42252" checksum="F14075ED4110C629" modified="2011-07-27" version="1">MKLEMLSPLHIGSGETLTPADFIANESEIIVLDLNKLFNMLISIGANIDEITEILETESYPWKKILKKYNLNPKEFERYSLKLIGNKGKYSMQVRAFIKSGGYPYIPGSSIKGAIRTAIMYKIVKNNPELLNQAVRKLYNEVKDLNSRGIGRVIKTADNWLEARIFGCNNRGYYEPKKDTLKGLIIRDSERISPRKLKVYGVWTIGERGTIPQYVEGLENVKIDFEFTIDENLLKLNYGEFNGTLKDINWEFIENALREFYEEVIKVELREIHKYGRYRDEVLEFYQDMQMKLESGEIPIRIGWGSGWYSTTLGVLLKTHPNFENLRRKLGLGRNPKTKKIVNDFPKTRRVANKKPLGWCFLKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEV7</accession>
    <name>F6BEV7_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DNA polymerase</fullName>
            <ecNumber evidence="5">2.7.7.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Metig_0133</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) = diphosphate + DNA(n+1)</text>
            <dbReference type="Rhea" id="RHEA:22508"/>
            <dbReference type="Rhea" id="RHEA-COMP:17339"/>
            <dbReference type="Rhea" id="RHEA-COMP:17340"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:173112"/>
            <dbReference type="EC" id="2.7.7.7"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the DNA polymerase type-B family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.7" evidence="5"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95693.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798302.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="SMR" id="F6BEV7"/>
    <dbReference type="STRING" id="880724.Metig_0133"/>
    <dbReference type="EnsemblBacteria" id="AEF95693">
        <property type="protein sequence ID" value="AEF95693"/>
        <property type="gene ID" value="Metig_0133"/>
    </dbReference>
    <dbReference type="GeneID" id="10642968"/>
    <dbReference type="KEGG" id="mig:Metig_0133"/>
    <dbReference type="HOGENOM" id="CLU_000203_6_0_2"/>
    <dbReference type="OMA" id="GNQKSPY"/>
    <dbReference type="OrthoDB" id="35869at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.132.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1600.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006172">
        <property type="entry name" value="DNA-dir_DNA_pol_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017964">
        <property type="entry name" value="DNA-dir_DNA_pol_B_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006133">
        <property type="entry name" value="DNA-dir_DNA_pol_B_exonuc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006134">
        <property type="entry name" value="DNA-dir_DNA_pol_B_multi_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042087">
        <property type="entry name" value="DNA_pol_B_thumb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023211">
        <property type="entry name" value="DNA_pol_palm_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00136">
        <property type="entry name" value="DNA_pol_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03104">
        <property type="entry name" value="DNA_pol_B_exo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00106">
        <property type="entry name" value="DNAPOLB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00486">
        <property type="entry name" value="POLBc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="SSF56672"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00116">
        <property type="entry name" value="DNA_POLYMERASE_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0235">DNA replication</keyword>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 5" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="3 5" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DNA_pol_B_exo1" evidence="7">
        <location>
            <begin position="28"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="domain" description="DNA_pol_B" evidence="6">
        <location>
            <begin position="355"/>
            <end position="754"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF03104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEF95693.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="781" mass="92242" checksum="BAF0F8B89A9765CE" modified="2011-07-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEY0</accession>
    <name>F6BEY0_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Ferrous iron transport protein B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">Metig_0158</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95716.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798325.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0158"/>
    <dbReference type="EnsemblBacteria" id="AEF95716">
        <property type="protein sequence ID" value="AEF95716"/>
        <property type="gene ID" value="Metig_0158"/>
    </dbReference>
    <dbReference type="GeneID" id="10642993"/>
    <dbReference type="KEGG" id="mig:Metig_0158"/>
    <dbReference type="HOGENOM" id="CLU_013350_3_0_2"/>
    <dbReference type="OMA" id="PLGYDWK"/>
    <dbReference type="OrthoDB" id="27688at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015093">
        <property type="term" value="F:ferrous iron transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01879">
        <property type="entry name" value="FeoB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003373">
        <property type="entry name" value="Fe2_transport_prot-B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011640">
        <property type="entry name" value="Fe2_transport_prot_B_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041069">
        <property type="entry name" value="FeoB_Cyto"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030389">
        <property type="entry name" value="G_FEOB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011642">
        <property type="entry name" value="Gate_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07664">
        <property type="entry name" value="FeoB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17910">
        <property type="entry name" value="FeoB_Cyto"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02421">
        <property type="entry name" value="FeoB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07670">
        <property type="entry name" value="Gate"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00437">
        <property type="entry name" value="feoB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51711">
        <property type="entry name" value="G_FEOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="278"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="304"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="339"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="383"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="417"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="449"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="507"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="557"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="590"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="617"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="domain" description="FeoB-type G" evidence="10">
        <location>
            <begin position="6"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51711"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AEF95716.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="647" mass="72784" checksum="5FA7C7B4417B38E3" modified="2011-07-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-09-29" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEY2</accession>
    <name>F6BEY2_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="3">AAA ATPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_0160</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the archaeal ATPase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95718.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798327.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF95718">
        <property type="protein sequence ID" value="AEF95718"/>
        <property type="gene ID" value="Metig_0160"/>
    </dbReference>
    <dbReference type="GeneID" id="10642995"/>
    <dbReference type="KEGG" id="mig:Metig_0160"/>
    <dbReference type="HOGENOM" id="CLU_068608_0_0_2"/>
    <dbReference type="OMA" id="WKFGVEE"/>
    <dbReference type="OrthoDB" id="34326at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011579">
        <property type="entry name" value="ATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01637">
        <property type="entry name" value="ATPase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA" evidence="2">
        <location>
            <begin position="21"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF95718.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="340" mass="40398" checksum="B9ACF50B815E17EB" modified="2011-07-27" version="1">MKFYNREKELQYLKTYCQLEPNSILFVYGPKSSGKTTVMLRVIEELSERGDLVFFYYDLRKYATPTKEEFLEIFFEKGDKKYLLNKLEINLKIFKLGVEENFDFNNLSLNDIFAKINESINAVVKDRKKPVLIIDELQKLKNIYFNGGKSLLNELFNLFVHLTKVRHLCHVVCLTSDTLFIEEIYQNSTLENTSKYYLIDWLKKEDIKKILKEEGFNDKEINYCLNYLSLPYEIVDLIENKKLGLSVEETIRQWINIEKDKILYLIANIPDELNENKINEILGKFKNNIKVKNEEIIKKEFINEFKFLVNNEILFYDVINGIIKPTSIKTWHSIRELLKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEY7</accession>
    <name>F6BEY7_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="4">L-seryl-tRNA(Sec) kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metig_0165</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95723.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798332.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0165"/>
    <dbReference type="EnsemblBacteria" id="AEF95723">
        <property type="protein sequence ID" value="AEF95723"/>
        <property type="gene ID" value="Metig_0165"/>
    </dbReference>
    <dbReference type="GeneID" id="10643000"/>
    <dbReference type="KEGG" id="mig:Metig_0165"/>
    <dbReference type="HOGENOM" id="CLU_1100964_0_0_2"/>
    <dbReference type="OMA" id="RESFPVW"/>
    <dbReference type="OrthoDB" id="67618at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016773">
        <property type="term" value="F:phosphotransferase activity, alcohol group as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097056">
        <property type="term" value="P:selenocysteinyl-tRNA(Sec) biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013641">
        <property type="entry name" value="KTI12/PSTK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020024">
        <property type="entry name" value="L-seryl-tRNA_Sec_kinase_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08433">
        <property type="entry name" value="KTI12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03574">
        <property type="entry name" value="selen_PSTK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="180"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEF95723.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="271" mass="32158" checksum="499B9A031EA6B444" modified="2011-07-27" version="1">MLIILVGLPSVGKTTFSKKLSKELHKMGIDNIVLGSDLIRESFPVWNEKYEEFIKEATYDLIDKALKKYTVIVDDTNYYNSKRRDLINIAKKNKKNYMIIYLHAPLEILLKRNVERGEKIPNEVIIKMFEKFDKPGEKYKWDEPHIVIDTTKEINIEEIAKLVKDYDRINKKNPKVVDNNKDHKENHEDAKNNKIKIMDKIDKITRKVVGEVISKQEPSKIKEISKELTKLRKEFLKEMNKKLDEENIDENYFSEEKIKEEFRRCISKLIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F6BF05</accession>
    <name>F6BF05_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Peptidase U62 modulator of DNA gyrase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Metig_0184</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase U62 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95741.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798350.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0184"/>
    <dbReference type="EnsemblBacteria" id="AEF95741">
        <property type="protein sequence ID" value="AEF95741"/>
        <property type="gene ID" value="Metig_0184"/>
    </dbReference>
    <dbReference type="GeneID" id="10643019"/>
    <dbReference type="KEGG" id="mig:Metig_0184"/>
    <dbReference type="HOGENOM" id="CLU_026425_1_2_2"/>
    <dbReference type="OMA" id="TDFWGSM"/>
    <dbReference type="OrthoDB" id="22382at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2290.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045569">
        <property type="entry name" value="Metalloprtase-TldD/E_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045570">
        <property type="entry name" value="Metalloprtase-TldD/E_cen_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002510">
        <property type="entry name" value="Metalloprtase-TldD/E_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025502">
        <property type="entry name" value="TldD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035068">
        <property type="entry name" value="TldD/PmbA_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036059">
        <property type="entry name" value="TldD/PmbA_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01523">
        <property type="entry name" value="PmbA_TldD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19289">
        <property type="entry name" value="PmbA_TldD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19290">
        <property type="entry name" value="PmbA_TldD_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004919">
        <property type="entry name" value="TldD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111283">
        <property type="entry name" value="SSF111283"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PmbA_TldD" evidence="3">
        <location>
            <begin position="18"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="domain" description="PmbA_TldD_M" evidence="5">
        <location>
            <begin position="112"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="domain" description="PmbA_TldD_C" evidence="4">
        <location>
            <begin position="224"/>
            <end position="425"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF19289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF19290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEF95741.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="451" mass="49420" checksum="83A7868D58B589ED" modified="2011-07-27" version="1">MDINIEKLEKLLEVGTYADIRINHGESNHIILKDGKIDEISSGAGGGVAVRVLYKNAWGFATSNVIDLKEIENLINKAYKMAKVSNEYTDKEVILKEVKAVVDKVKPKAKINPKDVNIDEKKEFVLLAHENMFGDKVVSTSVSYSDAVGYSLFMNSEGTRIESERMRTIMYLTAVAKDGTLQYASERVGGDGFEVIKNANIENIAKSAKERAIRLLYAKPCPKGKFKVILDPELTGVFIHEAVGHASEADLVLQNDSVFKDKIGEKVGSEYVTVIDSPEVEGFGSYKYDDEGVKGRETIIIENGILKNFLHTRETAGRLNMEVTGNARAEGLNRPIVRMSNTFIKPGDWDFEELLEDTKDGIFLKGSRGGQVDTGKGLFQFSAVEAFLIENGELTTPLKDAGLSGEILNILYNVDAVTKEFKLSVGYCGKNGQSVPVGDGGGSIRTIAILS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>F6BF48</accession>
    <name>F6BF48_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Transcriptional regulator, AsnC family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Metig_1383</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96918.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799514.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1383"/>
    <dbReference type="EnsemblBacteria" id="AEF96918">
        <property type="protein sequence ID" value="AEF96918"/>
        <property type="gene ID" value="Metig_1383"/>
    </dbReference>
    <dbReference type="GeneID" id="10644256"/>
    <dbReference type="KEGG" id="mig:Metig_1383"/>
    <dbReference type="HOGENOM" id="CLU_091233_5_2_2"/>
    <dbReference type="OMA" id="NIFCKIR"/>
    <dbReference type="OrthoDB" id="97695at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000485">
        <property type="entry name" value="AsnC-type_HTH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019888">
        <property type="entry name" value="Tscrpt_reg_AsnC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019887">
        <property type="entry name" value="Tscrpt_reg_AsnC/Lrp_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01037">
        <property type="entry name" value="AsnC_trans_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13404">
        <property type="entry name" value="HTH_AsnC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00033">
        <property type="entry name" value="HTHASNC"/>
    </dbReference>
    <dbReference type="SMART" id="SM00344">
        <property type="entry name" value="HTH_ASNC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="SSF54909"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50956">
        <property type="entry name" value="HTH_ASNC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH asnC-type" evidence="4">
        <location>
            <begin position="3"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEF96918.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="149" mass="16979" checksum="B05E4136FB272C21" modified="2011-07-27" version="1">MNLDKIDLKILRILQEDGRKSYREIGRELKISEGTVRARIKKMKENGIINGFHISVNPHKLGKDVIAILGLYLKPSEVKKTLEILKSYDEITQLYQTTGEYDAVCVVVLEDMEELGRFLSEKIYPLVNVNGCKVTLVLNILKDGHKIPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>F6BF57</accession>
    <name>F6BF57_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">F420_ligase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_1392</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96927.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1392"/>
    <dbReference type="EnsemblBacteria" id="AEF96927">
        <property type="protein sequence ID" value="AEF96927"/>
        <property type="gene ID" value="Metig_1392"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_1392"/>
    <dbReference type="HOGENOM" id="CLU_965090_0_0_2"/>
    <dbReference type="OMA" id="WSKYLWG"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002847">
        <property type="entry name" value="F420-0_gamma-glut_ligase-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012030">
        <property type="entry name" value="UCP006563"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01996">
        <property type="entry name" value="F420_ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006563">
        <property type="entry name" value="UCP006563"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="66"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="186"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="domain" description="F420_ligase" evidence="2">
        <location>
            <begin position="10"/>
            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01996"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF96927.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="286" mass="32555" checksum="F964C8459CC65D1F" modified="2011-07-27" version="1">MVEMMKAYPIKTRYIKPGENFIPIVIESLKNSNIKLNDGDFVVLSEKMVSTSENNFVDEREVKPKFWAYFTYYWSKYLWGYILGPLLGTRKDRVKNLRKMPKKETLRHKQVVIDHVGLIYALKPASEGGVDLTNVPGSYASLLPKEPKKWAEKLHKKIKEEFDVDVVVMVVDTDATYRFFKWYVTALPYAIDGIISGIGVLGYIIGKIGKRLGVGGLVGATPLAIAGNEIYKKYPLEKILKIADLADRSHITTKSIHDVMKKYNTFEITIEILEELEHTPVVVVKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>F6BF68</accession>
    <name>F6BF68_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF6293 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_1403</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96938.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1403"/>
    <dbReference type="EnsemblBacteria" id="AEF96938">
        <property type="protein sequence ID" value="AEF96938"/>
        <property type="gene ID" value="Metig_1403"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_1403"/>
    <dbReference type="HOGENOM" id="CLU_907966_0_0_2"/>
    <dbReference type="OMA" id="RRAEKVY"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046260">
        <property type="entry name" value="DUF6293"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19810">
        <property type="entry name" value="DUF6293"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="domain" description="DUF6293" evidence="2">
        <location>
            <begin position="45"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF19810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF96938.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="358" mass="42065" checksum="35033B72F071F91F" modified="2011-07-27" version="1">MVKYIKYNIHYISKLTILTILIILIIINVNCGETMDEVGKIRGGVHIAVQGYEVDRITEVPIKRRAEKVYLICMPEEEDSERGKEFKNMIIERFEKNKIDYEILEANIFNLDEIAKKIKYAINKERKEFEEVKFYINVSSGSTIGCIAGITCAMILNKENSKIIPYYVIPKKLLDSLSDEECKKLKEEYKYLPRSFGVKNVLLIYPFNISLPREELLIFLKFINRAGDRGLTIKELSILTREEFLDVNLNNEESIRRLIKAIEHINSNPSRIRIERYKRTIQKLLEIVGDDKEELNKIVNWRNRTKFSENKTPQSDLVWVNKNVVEKLKEFELIVDEKIGRSRYIKVSEKGKMLLNYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>F6BA74</accession>
    <name>F6BA74_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Endonuclease III</fullName>
            <ecNumber evidence="10">4.2.99.18</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">DNA-(apurinic or apyrimidinic site) lyase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">nth</name>
        <name evidence="13" type="ordered locus">Metig_0207</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">DNA repair enzyme that has both DNA N-glycosylase activity and AP-lyase activity. The DNA N-glycosylase activity releases various damaged pyrimidines from DNA by cleaving the N-glycosidic bond, leaving an AP (apurinic/apyrimidinic) site. The AP-lyase activity cleaves the phosphodiester bond 3' to the AP site by a beta-elimination, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>2'-deoxyribonucleotide-(2'-deoxyribose 5'-phosphate)-2'-deoxyribonucleotide-DNA = a 3'-end 2'-deoxyribonucleotide-(2,3-dehydro-2,3-deoxyribose 5'-phosphate)-DNA + a 5'-end 5'-monophospho-2'-deoxyribonucleoside-DNA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:66592"/>
            <dbReference type="Rhea" id="RHEA-COMP:13180"/>
            <dbReference type="Rhea" id="RHEA-COMP:16897"/>
            <dbReference type="Rhea" id="RHEA-COMP:17067"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:136412"/>
            <dbReference type="ChEBI" id="CHEBI:157695"/>
            <dbReference type="ChEBI" id="CHEBI:167181"/>
            <dbReference type="EC" id="4.2.99.18"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="10">Binds 1 [4Fe-4S] cluster.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the Nth/MutY family.</text>
    </comment>
    <dbReference type="EC" id="4.2.99.18" evidence="10"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95764.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798373.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0207"/>
    <dbReference type="EnsemblBacteria" id="AEF95764">
        <property type="protein sequence ID" value="AEF95764"/>
        <property type="gene ID" value="Metig_0207"/>
    </dbReference>
    <dbReference type="GeneID" id="10643042"/>
    <dbReference type="KEGG" id="mig:Metig_0207"/>
    <dbReference type="HOGENOM" id="CLU_805637_0_0_2"/>
    <dbReference type="OMA" id="YYFYVGS"/>
    <dbReference type="OrthoDB" id="124734at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140078">
        <property type="term" value="F:class I DNA-(apurinic or apyrimidinic site) endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019104">
        <property type="term" value="F:DNA N-glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00056">
        <property type="entry name" value="ENDO3c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10441">
        <property type="entry name" value="GIY-YIG_COG1833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1670.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00942">
        <property type="entry name" value="Nth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011257">
        <property type="entry name" value="DNA_glycosylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002837">
        <property type="entry name" value="DUF123"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004036">
        <property type="entry name" value="Endonuclease-III-like_CS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003651">
        <property type="entry name" value="Endonuclease3_FeS-loop_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000305">
        <property type="entry name" value="GIY-YIG_endonuc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003265">
        <property type="entry name" value="HhH-GPD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023170">
        <property type="entry name" value="HhH_base_excis_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000445">
        <property type="entry name" value="HhH_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005759">
        <property type="entry name" value="Nth"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01986">
        <property type="entry name" value="DUF123"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00633">
        <property type="entry name" value="HHH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00730">
        <property type="entry name" value="HhH-GPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00478">
        <property type="entry name" value="ENDO3c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00525">
        <property type="entry name" value="FES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00465">
        <property type="entry name" value="GIYc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48150">
        <property type="entry name" value="SSF48150"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01155">
        <property type="entry name" value="ENDONUCLEASE_III_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 10" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="4 10" id="KW-0227">DNA damage</keyword>
    <keyword evidence="8 10" id="KW-0234">DNA repair</keyword>
    <keyword evidence="10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9 10" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 10" id="KW-0408">Iron</keyword>
    <keyword evidence="7 10" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="10 13" id="KW-0456">Lyase</keyword>
    <keyword evidence="3 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ENDO3c" evidence="12">
        <location>
            <begin position="35"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="domain" description="GIY-YIG" evidence="11">
        <location>
            <begin position="247"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="184"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="190"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="193"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="201"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AEF95764.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="344" mass="40356" checksum="E881179488B0ACC9" modified="2011-07-27" version="1">MEKIEILKEKLNKNAVVTEIAKEKSPFKVLISTILSARTKDEVTEEVSKRLFEKVKNVDDLLKIDLEELEKLIYPVGFYRMKAKNLKKLAKILKENYNGKVPNELDELLKLPGVGRKTANLVITLAFDDYGICVDTHVHRICNRWGYVETENPEETEMELRKKLPKKYWKIINNLLVVFGREICSPTPKCEECFEEIRERCPYYKKIAYFDEILKKYEFEKVSKNNIPAEKGTYILKIKLNRGRKIKFGNKERFFRKGYYFYVGSALGKSMNLKNRIGRHLKKEKKKFWHIDYLLEFGDVKGIYISNKGCECEVAKDLAEVFKCVEGFGCSDCKCESHLFYMPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>F6BA80</accession>
    <name>F6BA80_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">CRISPR-associated exonuclease Cas4</fullName>
            <ecNumber evidence="2 9">3.1.12.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">Metig_0213</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA).</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="9">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="9">Mg(2+) or Mn(2+) required for ssDNA cleavage activity.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>iron-sulfur cluster</name>
            <dbReference type="ChEBI" id="CHEBI:30408"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the CRISPR-associated exonuclease Cas4 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.12.1" evidence="2 9"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95770.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0213"/>
    <dbReference type="EnsemblBacteria" id="AEF95770">
        <property type="protein sequence ID" value="AEF95770"/>
        <property type="gene ID" value="Metig_0213"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0213"/>
    <dbReference type="HOGENOM" id="CLU_133784_0_0_2"/>
    <dbReference type="OMA" id="ICKTKLW"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004527">
        <property type="term" value="F:exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.320.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013343">
        <property type="entry name" value="CRISPR-assoc_prot_Cas4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022765">
        <property type="entry name" value="Dna2/Cas4_DUF83"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011604">
        <property type="entry name" value="PDDEXK-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01930">
        <property type="entry name" value="Cas_Cas4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00372">
        <property type="entry name" value="cas4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0051">Antiviral defense</keyword>
    <keyword evidence="9" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 9" id="KW-0408">Iron</keyword>
    <keyword evidence="6 9" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="8 9" id="KW-0464">Manganese</keyword>
    <keyword evidence="4 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0540">Nuclease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cas_Cas4" evidence="10">
        <location>
            <begin position="26"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU365022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AEF95770.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="182" mass="21655" checksum="7B6C6638C552606B" modified="2011-07-27" version="1">MMIFFKVIVVFKMEPEYLEKELLIRGTEINYLFICKTKLWYFAKGITMEQESDFVDLGKFLHEKSYFGEEKEVQIGTINIDFIKRKNIIEIHEVKKGKSMEKAHEIQALYYLYYLKRYGIEAKAILNYPKLREIKEIILDGREGEVEGAIKEVEKIKSMPNPPKPKKSKICKKCSYYELCWI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>F6BA86</accession>
    <name>F6BA86_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0219</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95776.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798385.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0219"/>
    <dbReference type="EnsemblBacteria" id="AEF95776">
        <property type="protein sequence ID" value="AEF95776"/>
        <property type="gene ID" value="Metig_0219"/>
    </dbReference>
    <dbReference type="GeneID" id="10643054"/>
    <dbReference type="KEGG" id="mig:Metig_0219"/>
    <dbReference type="HOGENOM" id="CLU_2271061_0_0_2"/>
    <dbReference type="OMA" id="YQLFWLS"/>
    <dbReference type="OrthoDB" id="104530at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="49"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="82"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF95776.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="102" mass="11397" checksum="C74FEB05EE3B561D" modified="2011-07-27" version="1">MDYDKFQKEMEKNSHGDGISIGAGWTGEFTLYGFFVFSAFLLGRVFGKVPLVLLGIMVVGILLSFSPAIFTFEKENSNAINYQLFWLSVFCGVLALVIYSCM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>F6BA93</accession>
    <name>F6BA93_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0226</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95783.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0226"/>
    <dbReference type="EnsemblBacteria" id="AEF95783">
        <property type="protein sequence ID" value="AEF95783"/>
        <property type="gene ID" value="Metig_0226"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0226"/>
    <dbReference type="HOGENOM" id="CLU_135436_0_0_2"/>
    <dbReference type="OMA" id="THYGNIG"/>
    <dbReference type="OrthoDB" id="100163at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="140"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF95783.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="166" mass="19401" checksum="416B6FC8B7FD81ED" modified="2011-07-27" version="1">MRISKIKIFIFVLIFVKLIEVDAYYFDYIKVNEKQNVSVEFECDKIGNYTYKLHFIHYGNIKENMQINIYLNGNLVYKIGDSNDASPAHEKNVTVDITDYLKDGENVLRVEGISIKGNETYHPYYVLDEVYINEPIKSPISFRSVVLTLILTTFIILKMEILNKNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>F6BA95</accession>
    <name>F6BA95_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">UDP-glucuronate 4-epimerase</fullName>
            <ecNumber evidence="2">5.1.3.6</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0228</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EC" id="5.1.3.6" evidence="2"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95785.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798394.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0228"/>
    <dbReference type="EnsemblBacteria" id="AEF95785">
        <property type="protein sequence ID" value="AEF95785"/>
        <property type="gene ID" value="Metig_0228"/>
    </dbReference>
    <dbReference type="GeneID" id="10643063"/>
    <dbReference type="KEGG" id="mig:Metig_0228"/>
    <dbReference type="HOGENOM" id="CLU_007383_1_7_2"/>
    <dbReference type="OMA" id="GVRTSWG"/>
    <dbReference type="OrthoDB" id="43299at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050378">
        <property type="term" value="F:UDP-glucuronate 4-epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0413">Isomerase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Epimerase" evidence="1">
        <location>
            <begin position="6"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF95785.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="326" mass="38193" checksum="5F4C48F39988001C" modified="2011-07-27" version="1">MKYKNILVTGSAGFIGFHLSKYLLENYDDVNVIGIDNLNNYYNPMLKEKRNEILKNYENYHFIKLDFSDWDSLVENLKDREIDLIVHLGAQAGVRYSLENPWTYIKSNDMGTLNIFELARKLDIEKVVYASSSSVYGGNKKIPFSEDDRVDNPISLYAATKRSNELMAYTYHHLYGIKMIGLRFFTVYGEYGRPDMAFWKFTKNILLGKPIEVYNYGKMERDFTYISDIVDGIIKSIDKNFDYEIFNLGNDNPTNLEYAISLIEKYLGKKAIKDYKPIQPGDVERTWADLTKSRKLLGYDPKVRIEEGLKRFCEWFLKNKDWTLKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2020-08-12" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAK5</accession>
    <name>F6BAK5_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">DNA polymerase beta domain protein region</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1484</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97018.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF97018">
        <property type="protein sequence ID" value="AEF97018"/>
        <property type="gene ID" value="Metig_1484"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_1484"/>
    <dbReference type="HOGENOM" id="CLU_3194504_0_0_2"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002934">
        <property type="entry name" value="Polymerase_NTP_transf_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01909">
        <property type="entry name" value="NTP_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="SSF81301"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NTP_transf_2" evidence="1">
        <location>
            <begin position="2"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF97018.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="45" mass="5423" checksum="6B9E9E9DE9BB03AA" modified="2011-07-27" version="1">MHKKELKEKYKIKSIEIFGSYTRNEQKETSDVDILVEFYEALDLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAM9</accession>
    <name>F6BAM9_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_0287</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95843.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798452.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF95843">
        <property type="protein sequence ID" value="AEF95843"/>
        <property type="gene ID" value="Metig_0287"/>
    </dbReference>
    <dbReference type="GeneID" id="10643122"/>
    <dbReference type="KEGG" id="mig:Metig_0287"/>
    <dbReference type="HOGENOM" id="CLU_2893254_0_0_2"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF95843.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="62" mass="7387" checksum="8BA63B9F7F3A9C70" modified="2011-07-27" version="1">MEVKNPYVLKKGKCKICGKDIEIIENTEEVQKRLQSKNISLNIDFEICEECRRKEYLKRVVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAP2</accession>
    <name>F6BAP2_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ARMT1-like_dom domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0300</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95856.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798465.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0300"/>
    <dbReference type="EnsemblBacteria" id="AEF95856">
        <property type="protein sequence ID" value="AEF95856"/>
        <property type="gene ID" value="Metig_0300"/>
    </dbReference>
    <dbReference type="GeneID" id="10643135"/>
    <dbReference type="KEGG" id="mig:Metig_0300"/>
    <dbReference type="HOGENOM" id="CLU_071520_1_0_2"/>
    <dbReference type="OMA" id="FLLKAKC"/>
    <dbReference type="OrthoDB" id="78551at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036075">
        <property type="entry name" value="ARMT-1-like_metal-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002791">
        <property type="entry name" value="ARMT1-like_metal-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014444">
        <property type="entry name" value="PH1575-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01937">
        <property type="entry name" value="ARMT1-like_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006593">
        <property type="entry name" value="UCP006593"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111321">
        <property type="entry name" value="SSF111321"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ARMT1-like_dom" evidence="1">
        <location>
            <begin position="5"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF95856.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="290" mass="32802" checksum="875B5731B50D7C5F" modified="2011-07-27" version="1">MKIRPECAICITRQVVDAAREITDDEKKQFKLVKDCLGVIREVYGEDAVPAWMGTHVHRYLKKISGNDDPYKRLKEIANKIALNYLEKIKDYVEVDDDLERLRRKVKATIAGNVIDFGPYSTDIDIVSKIKETIEGDLKVDYSEELLKDLKSSKKVFYICDNAGEIVFDRILIEEIKKYVDEVVVAVKGKPILNDATLEDAKLVGIDKIAKVITTGSDIIGIILEECSEEFLKEFESSDLIIAKGMGNYESLTEYEDKINKPIYYILKAKCVPVAENIGVNVGDNVLLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAP4</accession>
    <name>F6BAP4_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Beta-lactamase domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0302</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95858.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798467.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0302"/>
    <dbReference type="EnsemblBacteria" id="AEF95858">
        <property type="protein sequence ID" value="AEF95858"/>
        <property type="gene ID" value="Metig_0302"/>
    </dbReference>
    <dbReference type="GeneID" id="10643137"/>
    <dbReference type="KEGG" id="mig:Metig_0302"/>
    <dbReference type="HOGENOM" id="CLU_030571_2_6_2"/>
    <dbReference type="OMA" id="WTPGHTF"/>
    <dbReference type="OrthoDB" id="103066at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="SSF56281"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Lactamase_B" evidence="1">
        <location>
            <begin position="21"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF95858.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="187" mass="21284" checksum="1DE4F99893D3BE0C" modified="2011-07-27" version="1">MIKLLYEGELVRENGVIKKASSSVTLIQTKNHNIIVDTSTRDKRELIIEELKKLNLEPKDIDVIINTHRHYDHIENNDLFKNAVIYASPYECEGCSLGYEFVSIDEFKDDEIEIIDTPGHTWGSISIIYKDYVIVGDAAPLKRNILENIPPGVRVSEKVALRSLQKIRSLKKHVITGHDGIVYADEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAQ7</accession>
    <name>F6BAQ7_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Metig_0315</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the UPF0718 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95871.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798480.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0315"/>
    <dbReference type="EnsemblBacteria" id="AEF95871">
        <property type="protein sequence ID" value="AEF95871"/>
        <property type="gene ID" value="Metig_0315"/>
    </dbReference>
    <dbReference type="GeneID" id="10643150"/>
    <dbReference type="KEGG" id="mig:Metig_0315"/>
    <dbReference type="HOGENOM" id="CLU_058185_0_0_2"/>
    <dbReference type="OMA" id="TVMAFMM"/>
    <dbReference type="OrthoDB" id="48767at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005524">
        <property type="entry name" value="DUF318"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03773">
        <property type="entry name" value="ArsP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="60"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="96"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="124"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="163"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="203"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="233"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="266"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="309"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="337"/>
            <end position="357"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006386"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AEF95871.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="358" mass="38449" checksum="803EC07FEFE7BD9E" modified="2011-07-27" version="1">MDAIYLLNVAIQTLIDYLNLNRVIALTIAFFMAGGIASMINKNFIIKYFGPDTPKHISYLVASVSGCLLAVCSCTILPLFAGIYKRGAGIGPATTFLFSGPAINVLAIFYSAALLGWGIGFTRAVFAILLSILIGLSMELIFKSHENKRRFRIGKANSISTRPGYQTAIFFLIQLAMLLIITASPKLVPILSVPLYGGFLVKHLLTIIFAVVLVVVVKKWFKTDEIKAWLRETFILTRMVVPLLLIGIAIAGILKAVIPPELIGQYVGGNSIMANFVASLIGALMYFATLTEVPIIKSLMELGMGVGPAMALLLAGPSLSIPTVLTISRILGVKKALTYLGLVVIFSTIAGLIAGLIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAR1</accession>
    <name>F6BAR1_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Formylmethanofuran dehydrogenase subunit E region</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0319</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95875.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798484.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0319"/>
    <dbReference type="EnsemblBacteria" id="AEF95875">
        <property type="protein sequence ID" value="AEF95875"/>
        <property type="gene ID" value="Metig_0319"/>
    </dbReference>
    <dbReference type="GeneID" id="10643155"/>
    <dbReference type="KEGG" id="mig:Metig_0319"/>
    <dbReference type="HOGENOM" id="CLU_112307_0_0_2"/>
    <dbReference type="OMA" id="PKLHAWY"/>
    <dbReference type="OrthoDB" id="62422at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003814">
        <property type="entry name" value="FmdEsu_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02663">
        <property type="entry name" value="FmdE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FmdE" evidence="1">
        <location>
            <begin position="23"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF95875.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="193" mass="21775" checksum="82A0AC89CDFF779A" modified="2011-07-27" version="1">MNISEMSKRVGIKKEDFEKVLDFHGCLSPGVLIGMQMFSLAKKVLDIDEKDRIYVTCETQNCLPDAFQVLGKCTTGNKGLRIVDTGKMAVTVNKWGREGEIIKGVRIILDAKKTKKFPKLHAWYLNIEKVPHDEVILDILNAGEDVYSYEFVDIIVPKKSKKKIKFCEICNEPFVQKGDETICLSCQGSVVIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAS3</accession>
    <name>F6BAS3_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Nucleic acid binding OB-fold tRNA/helicase-type</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0331</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95887.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798496.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0331"/>
    <dbReference type="EnsemblBacteria" id="AEF95887">
        <property type="protein sequence ID" value="AEF95887"/>
        <property type="gene ID" value="Metig_0331"/>
    </dbReference>
    <dbReference type="GeneID" id="10643168"/>
    <dbReference type="KEGG" id="mig:Metig_0331"/>
    <dbReference type="HOGENOM" id="CLU_415991_0_0_2"/>
    <dbReference type="OMA" id="NAFSRKW"/>
    <dbReference type="OrthoDB" id="8528at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004365">
        <property type="entry name" value="NA-bd_OB_tRNA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01336">
        <property type="entry name" value="tRNA_anti-codon"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0347">Helicase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="OB" evidence="1">
        <location>
            <begin position="72"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="domain" description="OB" evidence="1">
        <location>
            <begin position="185"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="domain" description="OB" evidence="1">
        <location>
            <begin position="286"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="domain" description="OB" evidence="1">
        <location>
            <begin position="404"/>
            <end position="469"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF95887.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="637" mass="72674" checksum="6C060785F4168F16" modified="2011-07-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAS5</accession>
    <name>F6BAS5_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DNA double-strand break repair protein Mre11</fullName>
            <ecNumber evidence="5">3.1.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">mre11</name>
        <name evidence="7" type="ordered locus">Metig_0333</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Part of the Rad50/Mre11 complex, which is involved in the early steps of DNA double-strand break (DSB) repair. The complex may facilitate opening of the processed DNA ends to aid in the recruitment of HerA and NurA. Mre11 binds to DSB ends and has both double-stranded 3'-5' exonuclease activity and single-stranded endonuclease activity.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="5">Binds 2 manganese ions per subunit.</text>
    </comment>
    <comment type="activity regulation">
        <text evidence="5">Nuclease activity is regulated by Rad50.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer. Forms a heterotetramer composed of two Mre11 subunits and two Rad50 subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the MRE11/RAD32 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="5"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95889.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798498.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0333"/>
    <dbReference type="EnsemblBacteria" id="AEF95889">
        <property type="protein sequence ID" value="AEF95889"/>
        <property type="gene ID" value="Metig_0333"/>
    </dbReference>
    <dbReference type="GeneID" id="10643170"/>
    <dbReference type="KEGG" id="mig:Metig_0333"/>
    <dbReference type="HOGENOM" id="CLU_026621_5_2_2"/>
    <dbReference type="OMA" id="YRQYNLD"/>
    <dbReference type="OrthoDB" id="25870at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008408">
        <property type="term" value="F:3'-5' exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045027">
        <property type="term" value="F:DNA end binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000403">
        <property type="term" value="F:Y-form DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006302">
        <property type="term" value="P:double-strand break repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00840">
        <property type="entry name" value="MPP_Mre11_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02044">
        <property type="entry name" value="Mre11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP_ApaH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032885">
        <property type="entry name" value="Mre11_archaea-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041796">
        <property type="entry name" value="Mre11_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="SSF56300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0227">DNA damage</keyword>
    <keyword evidence="4 5" id="KW-0234">DNA repair</keyword>
    <keyword evidence="1 5" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="5" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="3 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0464">Manganese</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0540">Nuclease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metallophos" evidence="6">
        <location>
            <begin position="1"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="5">
        <location>
            <position position="85"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="8"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="10"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="49"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="84"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="156"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="183"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="185"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEF95889.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="362" mass="42611" checksum="6A84A6081A694AD0" modified="2011-07-27" version="1">MQFVHIGDNHLGYRQYNLDERENDIYNSFNECIEKILEIRPDFVVHSGDLFEDIRPPIKALSTAMRGFSRLKEKGIPIYIVMGNHDIPKRRFQETPLVLLKNYIRTFVNKPYHIHGDVFIGGANYHSKIGKENLIERLKILENASKEYRKKILIMHQGINPYVPEYELEKSELPEFSYYAMGHIHNRIIDRLENGGVLAYSGSTEIMRKDEYKDYKKNGKGFYVVDMSGDFDKNDVDFVKVESVNVKCRDFFEIFIDSKEKFEEFLNALSNTSKPIVHGVIKREFVMLLGILKSNALWYNIRIVDDEDIEYDIPEHSSIEKLFAEYVKSKGLDADFVLNLHRKLINNEGWEEFVKEYYNKFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAU7</accession>
    <name>F6BAU7_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative signal transduction protein with CBS domains</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_1500</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97034.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799628.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1500"/>
    <dbReference type="EnsemblBacteria" id="AEF97034">
        <property type="protein sequence ID" value="AEF97034"/>
        <property type="gene ID" value="Metig_1500"/>
    </dbReference>
    <dbReference type="GeneID" id="10644373"/>
    <dbReference type="KEGG" id="mig:Metig_1500"/>
    <dbReference type="HOGENOM" id="CLU_656595_0_0_2"/>
    <dbReference type="OMA" id="ETWGAIQ"/>
    <dbReference type="OrthoDB" id="45411at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014651">
        <property type="entry name" value="UCP036983_2CBS_MJ1404"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036983">
        <property type="entry name" value="UCP_2CBS_MJ1404"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="SSF54631"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0129">CBS domain</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBS" evidence="2">
        <location>
            <begin position="10"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="2">
        <location>
            <begin position="68"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="2">
        <location>
            <begin position="133"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="2">
        <location>
            <begin position="211"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF97034.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="404" mass="45724" checksum="3393349C8E9F29B0" modified="2011-07-27" version="1">MLNEPVIEIATKEVVTVTPDTPITKAVGIMEENHFHNLVVVDERGELYLVTMHDLLLASSVYEHVGELMFKPHCIHKDTPVMDAVCELIDSGQRAAPIVDDKGKLVGIVTDYDIMKRAATSKILKDVEVSKIMTRNPITIHENESIGKARSLMRKHNIGRLIVVDDEENPIGIVTEDDILKKVFKPKRRMTVGEYSGEKTPRMGQPVKSIMSSPLITADVNATVADVAKLMQEYDIRGVPILKNKMLRGIVTRLDIMKYIADLKKGAMIEVELHGALDEEMKDLAERIIATEVKKMVKHAGRIHWIKINIKKEHDKGGTPYYHITVYVKTPNKLYVGDASPKLSKTKRLEAEGEDIGYISEKQRWDFIEVLKHALISVEKQIDADRERYHPKTVGKKLKEEEVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAU8</accession>
    <name>F6BAU8_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 7">Aspartate carbamoyltransferase regulatory chain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">pyrI</name>
        <name evidence="10" type="ordered locus">Metig_1501</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 7">Involved in allosteric regulation of aspartate carbamoyltransferase.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="7">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Contains catalytic and regulatory chains.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the PyrI family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97035.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799629.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1501"/>
    <dbReference type="EnsemblBacteria" id="AEF97035">
        <property type="protein sequence ID" value="AEF97035"/>
        <property type="gene ID" value="Metig_1501"/>
    </dbReference>
    <dbReference type="GeneID" id="10644374"/>
    <dbReference type="KEGG" id="mig:Metig_1501"/>
    <dbReference type="HOGENOM" id="CLU_128576_0_0_2"/>
    <dbReference type="OMA" id="CPNRNCI"/>
    <dbReference type="OrthoDB" id="101957at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009347">
        <property type="term" value="C:aspartate carbamoyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006207">
        <property type="term" value="P:'de novo' pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006221">
        <property type="term" value="P:pyrimidine nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00002">
        <property type="entry name" value="Asp_carb_tr_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020545">
        <property type="entry name" value="Asp_carbamoyltransf_reg_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002801">
        <property type="entry name" value="Asp_carbamoylTrfase_reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020542">
        <property type="entry name" value="Asp_carbamoyltrfase_reg_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036792">
        <property type="entry name" value="Asp_carbatrfase_reg_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036793">
        <property type="entry name" value="Asp_carbatrfase_reg_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35805">
        <property type="entry name" value="PTHR35805"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01948">
        <property type="entry name" value="PyrI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02748">
        <property type="entry name" value="PyrI_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54893">
        <property type="entry name" value="SSF54893"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57825">
        <property type="entry name" value="SSF57825"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00240">
        <property type="entry name" value="ATCase_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 7" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 7" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="PyrI" evidence="8">
        <location>
            <begin position="6"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="domain" description="PyrI_C" evidence="9">
        <location>
            <begin position="101"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="107"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="112"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="135"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="138"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AEF97035.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="152" mass="16978" checksum="FEB706BFC776CBA5" modified="2011-07-27" version="1">MKNRELKVKPIQNGTVIDHIKSGKALDVYNILKIPDDLPVMLAMNVPSKKMGKKDILKIEGLELGSDDVNKIALIAPDATINIIRNGEVVEKFKVNIPDYIEGLLKCTNPNCITNRENVEGKFVIESKNPLKIRCKYCEKFLSAIIYDGSPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAY2</accession>
    <name>F6BAY2_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="8">ABC-type transporter, integral membrane subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Metig_1535</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the binding-protein-dependent transport system permease family. FecCD subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97069.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799663.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1535"/>
    <dbReference type="EnsemblBacteria" id="AEF97069">
        <property type="protein sequence ID" value="AEF97069"/>
        <property type="gene ID" value="Metig_1535"/>
    </dbReference>
    <dbReference type="GeneID" id="10644408"/>
    <dbReference type="KEGG" id="mig:Metig_1535"/>
    <dbReference type="HOGENOM" id="CLU_013016_0_0_2"/>
    <dbReference type="OMA" id="AMVYWSF"/>
    <dbReference type="OrthoDB" id="36067at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3470.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037294">
        <property type="entry name" value="ABC_BtuC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000522">
        <property type="entry name" value="ABC_transptr_permease_BtuC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30472">
        <property type="entry name" value="PTHR30472"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01032">
        <property type="entry name" value="FecCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81345">
        <property type="entry name" value="SSF81345"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="15"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="69"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="102"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="134"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="166"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="207"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="258"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="295"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="322"/>
            <end position="343"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEF97069.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="348" mass="37989" checksum="3B1B1131A87D14B3" modified="2011-07-27" version="1">MDVVKKYSKYTKKRIMFGIALLIILFLSSICSLIVGNYELTVSQVISAFFGKGSQSVNLIIWNIRLPRIFAAIVAGMSLAVAGAVMQCILRNPLASPFTIGISHGAMFGACLAIIIFGVGGAESTGHIFINNPYIITIFAFIGALIGVFVVLLLAKLRNLTPEAMVLAGVAMSSLFTAGTTLIQYFADDLQLAAMVYWTFGDLSRPLWTEIYIMVAVMISALIYFIYKRWDYNALEAGEETAKSLGVNTERTRLIGMLIASLLTSVNVAFLGIIGFIGLICPHIVRIFIGGDYRFLIPISALFGAVLLLISDTLARTVMSPIELPVGILTSFMGAPMFLYLLIKMYRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>F6BB09</accession>
    <name>F6BB09_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">tRNA (pseudouridine(54)-N(1))-methyltransferase</fullName>
            <ecNumber evidence="5">2.1.1.257</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">trmY</name>
        <name evidence="6" type="ordered locus">Metig_1562</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Specifically catalyzes the N1-methylation of pseudouridine at position 54 (Psi54) in tRNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>pseudouridine(54) in tRNA + S-adenosyl-L-methionine = H(+) + N(1)-methylpseudouridine(54) in tRNA + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:55292"/>
            <dbReference type="Rhea" id="RHEA-COMP:14140"/>
            <dbReference type="Rhea" id="RHEA-COMP:14141"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:74890"/>
            <dbReference type="EC" id="2.1.1.257"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the methyltransferase superfamily. TrmY family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.257" evidence="5"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97096.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1562"/>
    <dbReference type="EnsemblBacteria" id="AEF97096">
        <property type="protein sequence ID" value="AEF97096"/>
        <property type="gene ID" value="Metig_1562"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_1562"/>
    <dbReference type="HOGENOM" id="CLU_107018_0_0_2"/>
    <dbReference type="OMA" id="HADHCII"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008175">
        <property type="term" value="F:tRNA methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd18087">
        <property type="entry name" value="TrmY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1280.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00587">
        <property type="entry name" value="tRNA_methyltr_TrmY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029028">
        <property type="entry name" value="Alpha/beta_knot_MTases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007158">
        <property type="entry name" value="TrmY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029026">
        <property type="entry name" value="tRNA_m1G_MTases_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40703">
        <property type="entry name" value="PTHR40703"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04013">
        <property type="entry name" value="Methyltrn_RNA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75217">
        <property type="entry name" value="SSF75217"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 5" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3 5" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0819">tRNA processing</keyword>
    <feature type="region of interest" description="S-adenosyl-L-methionine binding" evidence="5">
        <location>
            <begin position="183"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="137"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="160"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="193"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00587"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEF97096.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="207" mass="23828" checksum="76AE6562D0EF8DEC" modified="2011-07-27" version="1">MLREFIFKANKTITSSNINLKDLPGSCGRLDLVCRCVSSAFFLSHDIRRDVIFYAVFYGEPNPPVALKFVGNELKKVSPDERNIAIFIKKALKKFEETPENKKTEWTESTPGIYVAKKEFRDIILEKKNEGKRILYLHKEGKDFENVKINENEDVMFVLGDHIGIGEEDEKFLEDIGAEKVSLSPLELHADHCIVIVHNILDKRFKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>F6BB46</accession>
    <name>F6BB46_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">S-adenosylmethionine decarboxylase proenzyme</fullName>
            <shortName evidence="10">AdoMetDC</shortName>
            <shortName evidence="10">SAMDC</shortName>
            <ecNumber evidence="10">4.1.1.50</ecNumber>
        </recommendedName>
        <component>
            <recommendedName>
                <fullName evidence="10">S-adenosylmethionine decarboxylase beta chain</fullName>
            </recommendedName>
        </component>
        <component>
            <recommendedName>
                <fullName evidence="10">S-adenosylmethionine decarboxylase alpha chain</fullName>
            </recommendedName>
        </component>
    </protein>
    <gene>
        <name evidence="10" type="primary">speH</name>
        <name evidence="11" type="ordered locus">Metig_0375</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the decarboxylation of S-adenosylmethionine to S-adenosylmethioninamine (dcAdoMet), the propylamine donor required for the synthesis of the polyamines spermine and spermidine from the diamine putrescine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>H(+) + S-adenosyl-L-methionine = CO2 + S-adenosyl 3-(methylsulfanyl)propylamine</text>
            <dbReference type="Rhea" id="RHEA:15981"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57443"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="EC" id="4.1.1.50"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>pyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
        </cofactor>
        <text evidence="10">Binds 1 pyruvoyl group covalently per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="10">Amine and polyamine biosynthesis; S-adenosylmethioninamine biosynthesis; S-adenosylmethioninamine from S-adenosyl-L-methionine: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Heterotetramer of two alpha and two beta chains arranged as a dimer of alpha/beta heterodimers.</text>
    </comment>
    <comment type="PTM">
        <text evidence="10">Is synthesized initially as an inactive proenzyme. Formation of the active enzyme involves a self-maturation process in which the active site pyruvoyl group is generated from an internal serine residue via an autocatalytic post-translational modification. Two non-identical subunits are generated from the proenzyme in this reaction, and the pyruvate is formed at the N-terminus of the alpha chain, which is derived from the carboxyl end of the proenzyme. The post-translation cleavage follows an unusual pathway, termed non-hydrolytic serinolysis, in which the side chain hydroxyl group of the serine supplies its oxygen atom to form the C-terminus of the beta chain, while the remainder of the serine residue undergoes an oxidative deamination to produce ammonia and the pyruvoyl group blocking the N-terminus of the alpha chain.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the prokaryotic AdoMetDC family. Type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.50" evidence="10"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95931.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798540.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0375"/>
    <dbReference type="EnsemblBacteria" id="AEF95931">
        <property type="protein sequence ID" value="AEF95931"/>
        <property type="gene ID" value="Metig_0375"/>
    </dbReference>
    <dbReference type="GeneID" id="10643212"/>
    <dbReference type="KEGG" id="mig:Metig_0375"/>
    <dbReference type="HOGENOM" id="CLU_125470_2_3_2"/>
    <dbReference type="OMA" id="FMCGACD"/>
    <dbReference type="OrthoDB" id="106151at2157"/>
    <dbReference type="UniPathway" id="UPA00331">
        <property type="reaction ID" value="UER00451"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004014">
        <property type="term" value="F:adenosylmethionine decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006557">
        <property type="term" value="P:S-adenosylmethioninamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008295">
        <property type="term" value="P:spermidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.750">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00464">
        <property type="entry name" value="AdoMetDC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042286">
        <property type="entry name" value="AdoMetDC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003826">
        <property type="entry name" value="AdoMetDC_fam_prok"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042284">
        <property type="entry name" value="AdoMetDC_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016067">
        <property type="entry name" value="S-AdoMet_deCO2ase_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017716">
        <property type="entry name" value="S-AdoMet_deCOase_pro-enz"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33866">
        <property type="entry name" value="PTHR33866"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02675">
        <property type="entry name" value="AdoMet_dc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56276">
        <property type="entry name" value="SSF56276"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03330">
        <property type="entry name" value="SAM_DCase_Bsu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 10" id="KW-0068">Autocatalytic cleavage</keyword>
    <keyword evidence="2 10" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="7 10 11" id="KW-0456">Lyase</keyword>
    <keyword evidence="5 10" id="KW-0620">Polyamine biosynthesis</keyword>
    <keyword evidence="9 10" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 10" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="8 10" id="KW-0704">Schiff base</keyword>
    <keyword evidence="4 10" id="KW-0745">Spermidine biosynthesis</keyword>
    <keyword evidence="6 10" id="KW-0865">Zymogen</keyword>
    <feature type="chain" id="PRO_5023425630" description="S-adenosylmethionine decarboxylase beta chain" evidence="10">
        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5023425629" description="S-adenosylmethionine decarboxylase alpha chain" evidence="10">
        <location>
            <begin position="63"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with substrate; via pyruvic acid" evidence="10">
        <location>
            <position position="63"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor; for processing activity" evidence="10">
        <location>
            <position position="68"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor; for catalytic activity" evidence="10">
        <location>
            <position position="83"/>
        </location>
    </feature>
    <feature type="site" description="Cleavage (non-hydrolytic); by autolysis" evidence="10">
        <location>
            <begin position="62"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="modified residue" description="Pyruvic acid (Ser); by autocatalysis" evidence="10">
        <location>
            <position position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AEF95931.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="120" mass="13214" checksum="687B02A605A09937" modified="2011-07-27" version="1">MRYLGKHLILELWGCDPKALDDQEGIEKMLVDSVNACGATLICIKTHKFSPQGVTGVAVLAESHISIHTWPELGYAAIDVFTCGSHVEPANALKPIREFLKPKHLEVLDVKRGIILEEGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-09-29" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>F6BB79</accession>
    <name>F6BB79_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="3">AAA ATPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_0408</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the archaeal ATPase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95964.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798573.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF95964">
        <property type="protein sequence ID" value="AEF95964"/>
        <property type="gene ID" value="Metig_0408"/>
    </dbReference>
    <dbReference type="GeneID" id="10643245"/>
    <dbReference type="KEGG" id="mig:Metig_0408"/>
    <dbReference type="HOGENOM" id="CLU_068608_0_0_2"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011579">
        <property type="entry name" value="ATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01637">
        <property type="entry name" value="ATPase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA" evidence="2">
        <location>
            <begin position="23"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF95964.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="336" mass="39879" checksum="B4EC44009649AE29" modified="2011-07-27" version="1">MQIKFYDRERELNYLKTYCQLIPNSILFVYGPKSSGKSTVMLKVIEEFKDRDDLVFFYYDLRKYATPTKEEFLNIFFEKGDKKYLYNKLEIDLKIFKFGVEENFDFNNVSLNNVFAKINEDINAVVEEGKRPILIIDELQKLKSIYFNGSKSLLNELFNLFVHLTKVRHLCHVICLTSDSLFINEIHNNSSLAKASEYYLIDWLEKETIEKILKEEKFNEEEINYAINYISLPYEIELLKSNKQLGVSVKETIKNWINIEEERIRHIMRTNPNKKEDILKILKLFSDKIKIKSEDVGDEIFDALKILIENEILFYDVINGIIKPHSLTTYYAIKYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>F6BB96</accession>
    <name>F6BB96_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Desulfoferrodoxin ferrous iron-binding region</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Metig_0425</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the desulfoferrodoxin family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95981.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798590.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0425"/>
    <dbReference type="EnsemblBacteria" id="AEF95981">
        <property type="protein sequence ID" value="AEF95981"/>
        <property type="gene ID" value="Metig_0425"/>
    </dbReference>
    <dbReference type="GeneID" id="10643262"/>
    <dbReference type="KEGG" id="mig:Metig_0425"/>
    <dbReference type="HOGENOM" id="CLU_118960_2_0_2"/>
    <dbReference type="OMA" id="FEKKHTP"/>
    <dbReference type="OrthoDB" id="106274at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.730">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002742">
        <property type="entry name" value="Desulfoferrodoxin_Fe-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036073">
        <property type="entry name" value="Desulfoferrodoxin_Fe-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01880">
        <property type="entry name" value="Desulfoferrodox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49367">
        <property type="entry name" value="SSF49367"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00332">
        <property type="entry name" value="neela_ferrous"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0249">Electron transport</keyword>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Desulfoferrodox" evidence="6">
        <location>
            <begin position="13"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEF95981.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="116" mass="13724" checksum="499FC6A0DE6189B1" modified="2011-07-27" version="1">MNYFCGINRMKEGTDFEKKHTPFIECPEVVKADEYFEVKVTTGIPHPMEDGHFIQWIELFMSDIYLARVDFTQFMKPEIVFVVKAPSKGHDKFTLKALMRCNLHGIWEYEKEVKVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2020-06-17" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBC1</accession>
    <name>F6BBC1_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_1595</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97128.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF97128">
        <property type="protein sequence ID" value="AEF97128"/>
        <property type="gene ID" value="Metig_1595"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_1595"/>
    <dbReference type="HOGENOM" id="CLU_3263778_0_0_2"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF97128.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="41" mass="4822" checksum="F4CBA7365F7503DD" modified="2011-07-27" version="1">MVFKFIKKLSGVDKKLVSEGDKLFSKCEYINVYVHFRLPDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBE3</accession>
    <name>F6BBE3_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(NiFe)-hydrogenase-3-type complex Eha, membrane protein EhaH</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1617</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97150.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799743.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1617"/>
    <dbReference type="EnsemblBacteria" id="AEF97150">
        <property type="protein sequence ID" value="AEF97150"/>
        <property type="gene ID" value="Metig_1617"/>
    </dbReference>
    <dbReference type="GeneID" id="10644490"/>
    <dbReference type="KEGG" id="mig:Metig_1617"/>
    <dbReference type="HOGENOM" id="CLU_1253609_0_0_2"/>
    <dbReference type="OMA" id="EMFRTPG"/>
    <dbReference type="OrthoDB" id="75706at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017059">
        <property type="entry name" value="NiFe-hyd_3_EhaH_prd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10125">
        <property type="entry name" value="NADHdeh_related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036536">
        <property type="entry name" value="EhaH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="22"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="74"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="128"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="166"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="200"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF97150.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="222" mass="24313" checksum="B216CE61DD20AA46" modified="2011-07-27" version="1">MDENTLLNIVGNFHGYIPLGDIVFYITDFSIICFLIAIFFTIVVYLSKPEKQLEAKLHDFGDVFYDVSLKELKIRRMMAIFCGIATAGAMLTGDLFDYALFLALVGIANIGIVSAVKREWVLNAAYQYGIIAMVAGLPLFGSAAMILAKTGTLSLFELAKTNADLFYPKVLFAVGMAGETGIAPFYAAKAEMFRAPGAPYILMIHLSSLLIIVRTVEILLTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBE7</accession>
    <name>F6BBE7_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1621</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97154.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799747.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1621"/>
    <dbReference type="EnsemblBacteria" id="AEF97154">
        <property type="protein sequence ID" value="AEF97154"/>
        <property type="gene ID" value="Metig_1621"/>
    </dbReference>
    <dbReference type="GeneID" id="10644494"/>
    <dbReference type="KEGG" id="mig:Metig_1621"/>
    <dbReference type="HOGENOM" id="CLU_2366257_0_0_2"/>
    <dbReference type="OMA" id="MNEVPFM"/>
    <dbReference type="OrthoDB" id="110204at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019211">
        <property type="entry name" value="EhaL"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09877">
        <property type="entry name" value="DUF2104"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="35"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="68"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF97154.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="89" mass="9723" checksum="3BD93DC66713EF33" modified="2011-07-27" version="1">MLLNIIAFIVGICLGLVYSYSKYKAPYVEKRIDKLALASAIIGGLIFNLSPPIGSLFLGFPLGMRPGYGRMEFLIGVIIAVLIYLSLKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBF5</accession>
    <name>F6BBF5_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative signal transduction protein with CBS domains</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_1629</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97162.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799754.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1629"/>
    <dbReference type="EnsemblBacteria" id="AEF97162">
        <property type="protein sequence ID" value="AEF97162"/>
        <property type="gene ID" value="Metig_1629"/>
    </dbReference>
    <dbReference type="GeneID" id="10644502"/>
    <dbReference type="KEGG" id="mig:Metig_1629"/>
    <dbReference type="HOGENOM" id="CLU_1286363_0_0_2"/>
    <dbReference type="OMA" id="AIWACGG"/>
    <dbReference type="OrthoDB" id="61989at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016486">
        <property type="entry name" value="UCP006591_CBS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006591">
        <property type="entry name" value="UCP006591_CBS_MJ1004"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="SSF54631"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0129">CBS domain</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBS" evidence="2">
        <location>
            <begin position="7"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="2">
        <location>
            <begin position="69"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF97162.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="213" mass="24384" checksum="E34B19B6C5FE9FFE" modified="2011-07-27" version="1">MKVKELMDTNFLKVYPDYTVEEVAKLMHEKNRYSAPVVDEHDKLVGWVNAIDLLILNDEDKKKEIKEFMHDVDKVIVLNENDEAREAVIKIVKYKVVSIPVVNNEGKVVGIVRNCDITKTLAKLYDIPVYKLFKTLQEQLRGITWEELMEAAAIVTKQTTGEEITPEEYERRIKNATFGKAIWACGGLEKFFAGLIRIGEVALARKVAKKRGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBF7</accession>
    <name>F6BBF7_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">ThiamineS protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_1631</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97164.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1631"/>
    <dbReference type="EnsemblBacteria" id="AEF97164">
        <property type="protein sequence ID" value="AEF97164"/>
        <property type="gene ID" value="Metig_1631"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_1631"/>
    <dbReference type="HOGENOM" id="CLU_114601_9_4_2"/>
    <dbReference type="OrthoDB" id="124031at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016155">
        <property type="entry name" value="Mopterin_synth/thiamin_S_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003749">
        <property type="entry name" value="ThiS/MoaD-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02597">
        <property type="entry name" value="ThiS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54285">
        <property type="entry name" value="SSF54285"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF97164.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="66" mass="7543" checksum="B65F08C280E21FB0" modified="2011-07-27" version="1">MDMKVHVEFENITKVIEVEKGSKVGDLLKKLNLRNVIVMRNDEFLLDDDELIENDNIKIIKVSSGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBI1</accession>
    <name>F6BBI1_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Beta-lactamase domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0434</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95990.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798599.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0434"/>
    <dbReference type="EnsemblBacteria" id="AEF95990">
        <property type="protein sequence ID" value="AEF95990"/>
        <property type="gene ID" value="Metig_0434"/>
    </dbReference>
    <dbReference type="GeneID" id="10643271"/>
    <dbReference type="KEGG" id="mig:Metig_0434"/>
    <dbReference type="HOGENOM" id="CLU_017490_0_0_2"/>
    <dbReference type="OMA" id="HVKNNIH"/>
    <dbReference type="OrthoDB" id="72715at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008254">
        <property type="entry name" value="Flavodoxin/NO_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029039">
        <property type="entry name" value="Flavoprotein-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016440">
        <property type="entry name" value="Rubredoxin-O_OxRdtase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00258">
        <property type="entry name" value="Flavodoxin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005243">
        <property type="entry name" value="ROO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52218">
        <property type="entry name" value="SSF52218"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="SSF56281"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50902">
        <property type="entry name" value="FLAVODOXIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Flavodoxin-like" evidence="1">
        <location>
            <begin position="247"/>
            <end position="386"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF95990.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="388" mass="44537" checksum="046B826EF8AB09EC" modified="2011-07-27" version="1">MVVEIKKDIFWVGVVDWEIRDFHGYITSNGSTYNSYLIKDEKNVLIDTAKGYMFDELIRGISRIINPEDIDYVVVNHVETDHSGCVDKIVELSGATVITNEKGKEHLSLHYDTKDWDFIIVDSGDEISIGKRTLKFIKTPMLHWPDNMVTYCKEEKILFSNDAFGQHLASSERFDYEVGDEILEYAKEYFANILMPYKMLIPNAINSLKDLEIELICPSHGVIWKEKKDEIIKKYLDWANDKIENKAVIVYDTMYNSTKKIAHAIADGLMENGVKVAIYKISETPMNTIIREILDAKYVLIGSPTLNQNVLPNIAKFLAYIEGLKPTNKIGVAFGSYGWMEMATEKIKEVFRKLNFEIVDDECLKVKFVPKEEDLKKCYEFGLKLGSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2020-06-17" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBK2</accession>
    <name>F6BBK2_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_0455</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96011.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96011">
        <property type="protein sequence ID" value="AEF96011"/>
        <property type="gene ID" value="Metig_0455"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0455"/>
    <dbReference type="HOGENOM" id="CLU_2766083_0_0_2"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF96011.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="69" mass="7939" checksum="CCB0E9025A5EA04F" modified="2011-07-27" version="1">MGGSGVISERSDENLKTVRFLCPRQGQRHSRVADDAQVQYGYLLTNTHIGQTPYFFSLYLEFYVGLLFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2020-06-17" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBK4</accession>
    <name>F6BBK4_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0457</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96013.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798622.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96013">
        <property type="protein sequence ID" value="AEF96013"/>
        <property type="gene ID" value="Metig_0457"/>
    </dbReference>
    <dbReference type="GeneID" id="43324314"/>
    <dbReference type="KEGG" id="mig:Metig_0457"/>
    <dbReference type="HOGENOM" id="CLU_2433879_0_0_2"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96013.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="90" mass="10439" checksum="7A1CBAFEB4AF4497" modified="2011-07-27" version="1">MEKSINDIKTKYFSLGIALGMTIVIASYVLLNYGFEKWDEYPLNYSRVSEFKIYDDTLEINNISYGKNVLRMNNFIKPILGEGSVQREQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBS0</accession>
    <name>F6BBS0_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0237 protein Metig_1668</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_1668</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0237 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97200.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799791.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1668"/>
    <dbReference type="EnsemblBacteria" id="AEF97200">
        <property type="protein sequence ID" value="AEF97200"/>
        <property type="gene ID" value="Metig_1668"/>
    </dbReference>
    <dbReference type="GeneID" id="10644541"/>
    <dbReference type="KEGG" id="mig:Metig_1668"/>
    <dbReference type="HOGENOM" id="CLU_155669_0_1_2"/>
    <dbReference type="OMA" id="MIMLVDI"/>
    <dbReference type="OrthoDB" id="116913at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd04872">
        <property type="entry name" value="ACT_1ZPV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01054">
        <property type="entry name" value="UPF0237"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002912">
        <property type="entry name" value="ACT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022986">
        <property type="entry name" value="UPF0237_ACT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="SSF55021"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51671">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ACT" evidence="2">
        <location>
            <begin position="5"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF97200.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="90" mass="10246" checksum="0651B40FEE363FD1" modified="2011-07-27" version="1">MERVVITVTGKDRTGIVANISSVLAENNVNILDIRQTIMDDLFTMIMLVDISNAKEDFLTLKKKLNEVGEKIGVKVIAQHEDIFKYMHRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBV7</accession>
    <name>F6BBV7_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Cobalamin (Vitamin B12) biosynthesis CbiM protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Metig_1705</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97237.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799827.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1705"/>
    <dbReference type="EnsemblBacteria" id="AEF97237">
        <property type="protein sequence ID" value="AEF97237"/>
        <property type="gene ID" value="Metig_1705"/>
    </dbReference>
    <dbReference type="GeneID" id="10644578"/>
    <dbReference type="KEGG" id="mig:Metig_1705"/>
    <dbReference type="HOGENOM" id="CLU_052508_2_1_2"/>
    <dbReference type="OMA" id="PWGTSGH"/>
    <dbReference type="OrthoDB" id="92958at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000041">
        <property type="term" value="P:transition metal ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002751">
        <property type="entry name" value="CbiM/NikMN"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01891">
        <property type="entry name" value="CbiM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="7"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="40"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="72"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="104"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="138"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="169"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEF97237.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="210" mass="22346" checksum="0CDF6F2E1275ABA2" modified="2011-07-27" version="1">MHIPDGFMPLWECGVFWLISLAFIAMSLKWASKELDEKKVPLFAALAAGIFAIQAMNMPIPWGTSGHMVGGALTAIIFDSPWAGVLMLTLVLIVQGVFFADGGLLVMGANIFNMGIIGSFVGYYVFKALKNIGTPIAAFVAGWASLFLSAIVCAIEMAIAGTFPLDKGLYYMGLYHACIGIIEGAITAIVIAYLSNVKPDLIGAKSTVNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBW7</accession>
    <name>F6BBW7_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">5'-deoxyadenosine deaminase</fullName>
            <shortName evidence="1">5'-dA deaminase</shortName>
            <ecNumber evidence="1">3.5.4.41</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">5'-methylthioadenosine deaminase</fullName>
            <shortName evidence="1">MTA deaminase</shortName>
            <ecNumber evidence="1">3.5.4.31</ecNumber>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Adenosine deaminase</fullName>
            <ecNumber evidence="1">3.5.4.4</ecNumber>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">S-adenosylhomocysteine deaminase</fullName>
            <shortName evidence="1">SAH deaminase</shortName>
            <ecNumber evidence="1">3.5.4.28</ecNumber>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">dadD</name>
        <name evidence="3" type="ordered locus">Metig_1715</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the deamination of three SAM-derived enzymatic products, namely 5'-deoxyadenosine, S-adenosyl-L-homocysteine, and 5'-methylthioadenosine, to produce the inosine analogs. Can also deaminate adenosine. The preferred substrate for this enzyme is 5'-deoxyadenosine, but all these substrates are efficiently deaminated. Likely functions in a S-adenosyl-L-methionine (SAM) recycling pathway from S-adenosyl-L-homocysteine (SAH) produced from SAM-dependent methylation reactions. May also be involved in the recycling of 5'-deoxyadenosine, whereupon the 5'-deoxyribose moiety of 5'-deoxyinosine is further metabolized to deoxyhexoses used for the biosynthesis of aromatic amino acids in methanogens.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>5'-deoxyadenosine + H(+) + H2O = 5'-deoxyinosine + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:42892"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:82775"/>
            <dbReference type="EC" id="3.5.4.41"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>H(+) + H2O + S-adenosyl-L-homocysteine = NH4(+) + S-inosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:20716"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:57985"/>
            <dbReference type="EC" id="3.5.4.28"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>H(+) + H2O + S-methyl-5'-thioadenosine = NH4(+) + S-methyl-5'-thioinosine</text>
            <dbReference type="Rhea" id="RHEA:25025"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17509"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:48595"/>
            <dbReference type="EC" id="3.5.4.31"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>adenosine + H(+) + H2O = inosine + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:24408"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16335"/>
            <dbReference type="ChEBI" id="CHEBI:17596"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="EC" id="3.5.4.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; S-adenosyl-L-methionine biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homotetramer.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="1">SAH is a product of SAM methyltransferases and is known to be a feedback inhibitor of these enzymes. As a result of this inhibition, organisms have evolved efficient enzymes to metabolize SAH via different pathways. The pathway found in methanogens differs from the canonical pathway, it uses the deamination of S-adenosyl-L-homocysteine to form S-inosyl-L-homocysteine for the regeneration of SAM from S-adenosyl-L-homocysteine. 5'-deoxyadenosine is a radical SAM enzyme reaction product which strongly inhibits radical SAM enzymes. A pathway for removing this product must be present in methanogens where the MTA/SAH nucleosidase which normally metabolizes this compound is absent.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the metallo-dependent hydrolases superfamily. MTA/SAH deaminase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="1">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.41" evidence="1"/>
    <dbReference type="EC" id="3.5.4.31" evidence="1"/>
    <dbReference type="EC" id="3.5.4.4" evidence="1"/>
    <dbReference type="EC" id="3.5.4.28" evidence="1"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97247.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799836.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1715"/>
    <dbReference type="EnsemblBacteria" id="AEF97247">
        <property type="protein sequence ID" value="AEF97247"/>
        <property type="gene ID" value="Metig_1715"/>
    </dbReference>
    <dbReference type="GeneID" id="10644589"/>
    <dbReference type="KEGG" id="mig:Metig_1715"/>
    <dbReference type="HOGENOM" id="CLU_012358_2_1_2"/>
    <dbReference type="OMA" id="FCPVERL"/>
    <dbReference type="OrthoDB" id="40873at2157"/>
    <dbReference type="UniPathway" id="UPA00315"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090613">
        <property type="term" value="F:5'-deoxyadenosine deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090614">
        <property type="term" value="F:5'-methylthioadenosine deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004000">
        <property type="term" value="F:adenosine deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050270">
        <property type="term" value="F:S-adenosylhomocysteine deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006556">
        <property type="term" value="P:S-adenosylmethionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01281">
        <property type="entry name" value="MTA_SAH_deamin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006680">
        <property type="entry name" value="Amidohydro-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023512">
        <property type="entry name" value="Deaminase_MtaD/DadD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011059">
        <property type="entry name" value="Metal-dep_hydrolase_composite"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01979">
        <property type="entry name" value="Amidohydro_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51338">
        <property type="entry name" value="SSF51338"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="SSF51556"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Amidohydro-rel" evidence="2">
        <location>
            <begin position="62"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="69"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="71"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="98"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="190"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="217"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="220"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF97247.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="428" mass="48580" checksum="4E82F1D79293471B" modified="2011-07-27" version="1">MILIKNLSYLIDKDLKVYENCDVLIKKEEDGIKKIVGKNLIEKENLNKSDLKIIDGKNKAGMPGLVNTHTHIPMTLLRGIADDMRLEEWLREKIWPNEAKLTKEDVYYGSLLGCLEMLRFGVTIFNDMYFFAEKVAEATKEIGLRGVISFPIIDVGTPECENRDKLIRMAENFIKKYKEDKLIKPAIAPHAPYTCSEETYKECKEIADNYGTLLHTHLSETRYEVVEMENKIGLRPIEYLEKIGVLDNNVIAAHCVWVTKEEVRKLAKHKVKVSHCPISNMKLASGGVMPLIEMLNDGVEITIGTDGVASNNNLDMLEEIKVCSILHKAHRWDATVGDIDTVLKMAFNSDALGIENNDIVLIDLTAPHLRPIHNIKSNIVYSANGNDVDTVIANGEVLMENKRFKCIDEKFLDKLYEKVDKIKEKFSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBY6</accession>
    <name>F6BBY6_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Resolvase helix-turn-helix domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_0511</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96067.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798675.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96067">
        <property type="protein sequence ID" value="AEF96067"/>
        <property type="gene ID" value="Metig_0511"/>
    </dbReference>
    <dbReference type="GeneID" id="10643348"/>
    <dbReference type="KEGG" id="mig:Metig_0511"/>
    <dbReference type="HOGENOM" id="CLU_101656_0_0_2"/>
    <dbReference type="OMA" id="QFMELKV"/>
    <dbReference type="OrthoDB" id="116373at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF96067.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="172" mass="20856" checksum="9A666DC4BD9C7B77" modified="2011-07-27" version="1">MIEIDLNKIVNWKEIERIKNLSKKDFVLVRIPKGVYENKKMKYKIEMLKKEPTIYLEIKTKKRGRKKKVDDPIKQKIINLIKEGYSIREVGKELGISKSTVWEYAKETIKEMKKEELMQLVWKYKEYLIKNELYTPQVQILFSELEMHIKNNDFENTHKKLKEIIKYTNEDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F6BC00</accession>
    <name>F6BC00_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transposase, IS240</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0526</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96081.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798689.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0526"/>
    <dbReference type="EnsemblBacteria" id="AEF96081">
        <property type="protein sequence ID" value="AEF96081"/>
        <property type="gene ID" value="Metig_0526"/>
    </dbReference>
    <dbReference type="GeneID" id="10643364"/>
    <dbReference type="KEGG" id="mig:Metig_0526"/>
    <dbReference type="HOGENOM" id="CLU_079548_0_0_2"/>
    <dbReference type="OMA" id="FDFRYEK"/>
    <dbReference type="OrthoDB" id="101978at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032874">
        <property type="entry name" value="DDE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13610">
        <property type="entry name" value="DDE_Tnp_IS240"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DDE_Tnp_IS240" evidence="1">
        <location>
            <begin position="82"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13610"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96081.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="214" mass="25267" checksum="2FDEC3CA3CEA514D" modified="2011-07-27" version="1">MELTIEVIKERIKEKKLFKRNKIPVEIKILAGLLYYLGLSLRKTSSFLSQFGKISHESVRTYYHTIKEVLNEPERKTRNLIAIDETKLKVGDKTIYVWSAIDVETKECLGVYVSESRSSLDTISFVRSILKFCSNKPKILVDGRTWYPWALQKLGLEFERIRFGLRNCVESFFSVLKGRTKRFFNRFPMNSSFKTVVSWVKSFIMFYNWWKSLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>F6BC08</accession>
    <name>F6BC08_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Elongation factor Tu domain 2 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Metig_0534</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96089.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798696.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0534"/>
    <dbReference type="EnsemblBacteria" id="AEF96089">
        <property type="protein sequence ID" value="AEF96089"/>
        <property type="gene ID" value="Metig_0534"/>
    </dbReference>
    <dbReference type="GeneID" id="10643372"/>
    <dbReference type="KEGG" id="mig:Metig_0534"/>
    <dbReference type="HOGENOM" id="CLU_077867_0_0_2"/>
    <dbReference type="OMA" id="MMDYALV"/>
    <dbReference type="OrthoDB" id="76962at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015256">
        <property type="entry name" value="eIF2g_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009001">
        <property type="entry name" value="Transl_elong_EF1A/Init_IF2_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09173">
        <property type="entry name" value="eIF2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="SSF50447"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50465">
        <property type="entry name" value="SSF50465"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GTP_EFTU_D2" evidence="4">
        <location>
            <begin position="158"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="domain" description="eIF2_C" evidence="5">
        <location>
            <begin position="247"/>
            <end position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF03144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF09173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEF96089.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="309" mass="34672" checksum="E651AD4929FDA3CC" modified="2011-07-27" version="1">MKNISVGLFGDIENVGRELGKKGTSTDITLYNYKVGDDAVLYVEPTRYPDRIQPLIYTINMTDYALVFVDEIKPELGETLLALDMFGVENGTFVVGEYVDVEQLKAIISTTSMKNFDIMEKDFIKIREKMANLEIKKENSSEFTKIPIDHFFTVKSVGTVILGKVEKGEVKVHDKLRLYPTDKEVMVRSIQVHDKDVKEAGLGSRVGLALKGVTTDELERGMILSNGNLEVSDEISLNIKWNPFMQKEVKAGEEYQIICGLQAVSCKVVERENDNITLKLKKPIVYEKGEKIVLLDGSAKIRILGVGEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>F6BC22</accession>
    <name>F6BC22_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="3">APHP domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_0549</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96103.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798710.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0549"/>
    <dbReference type="EnsemblBacteria" id="AEF96103">
        <property type="protein sequence ID" value="AEF96103"/>
        <property type="gene ID" value="Metig_0549"/>
    </dbReference>
    <dbReference type="GeneID" id="10643387"/>
    <dbReference type="KEGG" id="mig:Metig_0549"/>
    <dbReference type="HOGENOM" id="CLU_277278_0_0_2"/>
    <dbReference type="OMA" id="DINGYKF"/>
    <dbReference type="OrthoDB" id="4820at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011635">
        <property type="entry name" value="CARDB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008965">
        <property type="entry name" value="CBM2/CBM3_carb-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022651">
        <property type="entry name" value="S_layer_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07705">
        <property type="entry name" value="CARDB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05124">
        <property type="entry name" value="S_layer_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49384">
        <property type="entry name" value="SSF49384"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CARDB" evidence="2">
        <location>
            <begin position="299"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="domain" description="CARDB" evidence="2">
        <location>
            <begin position="542"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="domain" description="S_layer_C" evidence="1">
        <location>
            <begin position="1029"/>
            <end position="1121"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF96103.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="1147" mass="126813" checksum="434C35558AA68845" modified="2011-07-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>F6BC31</accession>
    <name>F6BC31_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Regulatory protein ArsR</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0558</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96112.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798719.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0558"/>
    <dbReference type="EnsemblBacteria" id="AEF96112">
        <property type="protein sequence ID" value="AEF96112"/>
        <property type="gene ID" value="Metig_0558"/>
    </dbReference>
    <dbReference type="GeneID" id="10643396"/>
    <dbReference type="KEGG" id="mig:Metig_0558"/>
    <dbReference type="HOGENOM" id="CLU_097806_9_1_2"/>
    <dbReference type="OMA" id="WYVYELA"/>
    <dbReference type="OrthoDB" id="111804at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001845">
        <property type="entry name" value="HTH_ArsR_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01022">
        <property type="entry name" value="HTH_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00418">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH arsR-type" evidence="1">
        <location>
            <begin position="18"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96112.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="103" mass="12045" checksum="1BAFAE53A62EFEA2" modified="2011-07-27" version="1">MDFKINGGEKLSLGKIVKIGDALSNPIRIKILYLLNQRPWNIYELAKELNLSRPVVYAHLKKLEDADLVESDLVLEDVRAKRIYKARKFRFEIDNDTIDKLFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>F6BC72</accession>
    <name>F6BC72_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Tryptophan halogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_1746</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97278.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799867.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1746"/>
    <dbReference type="EnsemblBacteria" id="AEF97278">
        <property type="protein sequence ID" value="AEF97278"/>
        <property type="gene ID" value="Metig_1746"/>
    </dbReference>
    <dbReference type="GeneID" id="10644620"/>
    <dbReference type="KEGG" id="mig:Metig_1746"/>
    <dbReference type="HOGENOM" id="CLU_563393_0_0_2"/>
    <dbReference type="OMA" id="PRSMGIT"/>
    <dbReference type="OrthoDB" id="22033at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF97278.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="463" mass="52711" checksum="A00ED07349A587AA" modified="2011-07-27" version="1">MKIIIVGGGTSGLLSALALEKEGHDVVVLEKDEIGGLCRSEKINGYTMDIGVHAITMLNNGPLIRLLNKYARFIPNFKEYGDYYVRENNELYKIPVSMQEWMTTPLIPHKDKILITTKIMDLMTTGFTKDTSVYDVVSEMGLSDVSIKFFDTICYFLSGEDMKNTPLWRLFTGAGYIPEDDILPFIYNDLKINPMKSILVRKFSPEKIKKILSDGFLTTIKNSSKKYLNKFVNRSRYWTQGYPVGGVQSICDCILYSLNNTTIKNEEVISIEEEKNGYVVGTKEDNYYADVVIYSAPSRYLPDIAKIDEIQKLKDKLRNIKFTKSLTIWIGHDENPFSYLGSEVWINPPCWATCVTNYDKFLAPKGKHLMGFSFVNMKKEHALSAIENKLGIDVDKVDMIHVQKAIPEQASCCIGQFFVYPKIKDNFYVVGTDADPRSMGITRAAYSVEVMLSEFKCVFKNRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F6BC98</accession>
    <name>F6BC98_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Phosphoesterase PA-phosphatase related protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Metig_1772</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97304.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799893.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1772"/>
    <dbReference type="EnsemblBacteria" id="AEF97304">
        <property type="protein sequence ID" value="AEF97304"/>
        <property type="gene ID" value="Metig_1772"/>
    </dbReference>
    <dbReference type="GeneID" id="10644646"/>
    <dbReference type="KEGG" id="mig:Metig_1772"/>
    <dbReference type="HOGENOM" id="CLU_1318521_0_0_2"/>
    <dbReference type="OMA" id="WGGLMWY"/>
    <dbReference type="OrthoDB" id="57852at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026841">
        <property type="entry name" value="Aur1/Ipt1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036938">
        <property type="entry name" value="P_Acid_Pase_2/haloperoxi_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000326">
        <property type="entry name" value="P_Acid_Pase_2/haloperoxidase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14378">
        <property type="entry name" value="PAP2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00014">
        <property type="entry name" value="acidPPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48317">
        <property type="entry name" value="SSF48317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="67"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="97"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="168"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="domain" description="acidPPc" evidence="6">
        <location>
            <begin position="64"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEF97304.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="241" mass="27908" checksum="0A35C3F19F78497C" modified="2011-07-27" version="1">MERNRIIIRLLLYPFAYLMWGGLMWYSQIVNPIDLTHYLSYIPFCNAEFYATLQGLPPQIIEFFRVIYLYSFTFCIVGGIGYYLLIKKNFLKSDIMLVDLALGWLFAGLIYTFFVVQAPFDVGVAKDLVDFKLMWICTKPTYEIPSLHTAYSILIALHFKEEEGDIKYIFYALAILIPISTLIMGQHWIVDVITGILYGFFLYKFPKTIHIKIHKGIDFICGYIKPCTNCAHGVDNGKINK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCD5</accession>
    <name>F6BCD5_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="4">RNA-binding, CRM domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metig_0592</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96146.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798753.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0592"/>
    <dbReference type="EnsemblBacteria" id="AEF96146">
        <property type="protein sequence ID" value="AEF96146"/>
        <property type="gene ID" value="Metig_0592"/>
    </dbReference>
    <dbReference type="GeneID" id="10643430"/>
    <dbReference type="KEGG" id="mig:Metig_0592"/>
    <dbReference type="HOGENOM" id="CLU_095994_2_1_2"/>
    <dbReference type="OMA" id="FKPREGW"/>
    <dbReference type="OrthoDB" id="114057at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.110.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001890">
        <property type="entry name" value="RNA-binding_CRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017924">
        <property type="entry name" value="RNA-binding_YhbY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035920">
        <property type="entry name" value="YhbY-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01985">
        <property type="entry name" value="CRS1_YhbY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01103">
        <property type="entry name" value="CRS1_YhbY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75471">
        <property type="entry name" value="SSF75471"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00253">
        <property type="entry name" value="RNA_bind_YhbY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51295">
        <property type="entry name" value="CRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="CRM" evidence="3">
        <location>
            <begin position="11"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEF96146.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="134" mass="15787" checksum="37C305B799F341AD" modified="2011-07-27" version="1">MENNENQVSQKRLTSKAKRILRAKSHEIKPVVWIGKEGVDKVIEEVKRQLKDRGLIKVKIRRTALIYMDKKEIAEKLAKETDSEIVSIVGHVITLFRPREGWKRYTSKKTKKEKEKEKAEQFIAEFEKLRKGKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCD6</accession>
    <name>F6BCD6_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">30S ribosomal protein S19e</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">rps19e</name>
        <name evidence="5" type="ordered locus">Metig_0593</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">May be involved in maturation of the 30S ribosomal subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Part of the 30S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein eS19 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96147.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798754.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0593"/>
    <dbReference type="EnsemblBacteria" id="AEF96147">
        <property type="protein sequence ID" value="AEF96147"/>
        <property type="gene ID" value="Metig_0593"/>
    </dbReference>
    <dbReference type="GeneID" id="10643431"/>
    <dbReference type="KEGG" id="mig:Metig_0593"/>
    <dbReference type="HOGENOM" id="CLU_108559_1_0_2"/>
    <dbReference type="OMA" id="YIDGPVG"/>
    <dbReference type="OrthoDB" id="92409at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01474">
        <property type="entry name" value="Ribosomal_S19e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001266">
        <property type="entry name" value="Ribosomal_S19e"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027548">
        <property type="entry name" value="Ribosomal_S19e_archaeal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018277">
        <property type="entry name" value="Ribosomal_S19e_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11710">
        <property type="entry name" value="PTHR11710"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01090">
        <property type="entry name" value="Ribosomal_S19e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01413">
        <property type="entry name" value="Ribosomal_S19e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00628">
        <property type="entry name" value="RIBOSOMAL_S19E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 5" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEF96147.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="148" mass="16829" checksum="D9A6E0BCF885D706" modified="2011-07-27" version="1">MVTVYDVPPALLIEKTAKKLKEMGLKEPSWTPFVKTGAHKERRPENPDWWYIRCASILRKIYINGPVGVERLRTAYGGRKNRGHAPEHFVKGSGNIIRKALQELERLGLVAKTKEGRVITPKGQSLLDNTAKEVRDEIINEIPELAKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCG6</accession>
    <name>F6BCG6_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 6">V-type ATP synthase subunit F</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">V-ATPase subunit F</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">atpF</name>
        <name evidence="7" type="ordered locus">Metig_0627</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 6">Produces ATP from ADP in the presence of a proton gradient across the membrane.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the V-ATPase F subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96177.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0627"/>
    <dbReference type="EnsemblBacteria" id="AEF96177">
        <property type="protein sequence ID" value="AEF96177"/>
        <property type="gene ID" value="Metig_0627"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0627"/>
    <dbReference type="HOGENOM" id="CLU_135754_2_0_2"/>
    <dbReference type="OMA" id="QIPDKFG"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042777">
        <property type="term" value="P:proton motive force-driven plasma membrane ATP synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10580">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00312">
        <property type="entry name" value="ATP_synth_F_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008218">
        <property type="entry name" value="ATPase_V1-cplx_f_g_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022944">
        <property type="entry name" value="ATPase_V1-cplx_fsu_bac/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036906">
        <property type="entry name" value="ATPase_V1_fsu_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01990">
        <property type="entry name" value="ATP-synt_F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF159468">
        <property type="entry name" value="SSF159468"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0066">ATP synthesis</keyword>
    <keyword evidence="6" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="5 6" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020758"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEF96177.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="101" mass="11237" checksum="E2CF6C9AF920B1F3" modified="2011-07-27" version="1">MKMKIGVVGDRDTAIGFRLAGLVDVYEVNNKEEAAKAIKELAEREDIGLIITTERIGEEIRDVINSVEKVVVEIPDKNGPIVREHDPIRTLVRKAVGVDMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCI2</accession>
    <name>F6BCI2_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">2-amino-3,7-dideoxy-D-threo-hept-6-ulosonate synthase</fullName>
            <shortName evidence="2">ADH synthase</shortName>
            <shortName evidence="2">ADHS</shortName>
            <shortName evidence="2">ADTH synthase</shortName>
            <ecNumber evidence="2">2.2.1.10</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">aroA'</name>
        <name evidence="3" type="ordered locus">Metig_0643</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes a transaldol reaction between 6-deoxy-5-ketofructose 1-phosphate (DKFP) and L-aspartate semialdehyde (ASA) with an elimination of hydroxypyruvaldehyde phosphate to yield 2-amino-3,7-dideoxy-D-threo-hept-6-ulosonate (ADH). Plays a key role in an alternative pathway of the biosynthesis of 3-dehydroquinate (DHQ), which is involved in the canonical pathway for the biosynthesis of aromatic amino acids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>1-deoxy-D-threo-hexo-2,5-diulose 6-phosphate + L-aspartate 4-semialdehyde = 2,3-dioxopropyl phosphate + 2-amino-2,3,7-trideoxy-D-lyxo-hept-6-ulosonate</text>
            <dbReference type="Rhea" id="RHEA:25952"/>
            <dbReference type="ChEBI" id="CHEBI:58859"/>
            <dbReference type="ChEBI" id="CHEBI:58860"/>
            <dbReference type="ChEBI" id="CHEBI:58861"/>
            <dbReference type="ChEBI" id="CHEBI:537519"/>
            <dbReference type="EC" id="2.2.1.10"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodecamer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DeoC/FbaB aldolase family. ADHS subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.2.1.10" evidence="2"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96193.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798797.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0643"/>
    <dbReference type="EnsemblBacteria" id="AEF96193">
        <property type="protein sequence ID" value="AEF96193"/>
        <property type="gene ID" value="Metig_0643"/>
    </dbReference>
    <dbReference type="GeneID" id="10643483"/>
    <dbReference type="KEGG" id="mig:Metig_0643"/>
    <dbReference type="HOGENOM" id="CLU_057069_2_0_2"/>
    <dbReference type="OMA" id="CEYWGMP"/>
    <dbReference type="OrthoDB" id="57391at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004332">
        <property type="term" value="F:fructose-bisphosphate aldolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016836">
        <property type="term" value="F:hydro-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016744">
        <property type="term" value="F:transketolase or transaldolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009073">
        <property type="term" value="P:aromatic amino acid family biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:cellular amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00958">
        <property type="entry name" value="DhnA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00960">
        <property type="entry name" value="ADH_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010210">
        <property type="entry name" value="ADH_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002915">
        <property type="entry name" value="DeoC/FbaB/LacD_aldolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041720">
        <property type="entry name" value="FbaB-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01791">
        <property type="entry name" value="DeoC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038992">
        <property type="entry name" value="Aldolase_Ia"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01133">
        <property type="entry name" value="DeoC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01949">
        <property type="entry name" value="AroFGH_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="2" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="3" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0704">Schiff base</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="DKFP binding" evidence="2">
        <location>
            <begin position="33"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="DKFP binding" evidence="2">
        <location>
            <begin position="153"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="region of interest" description="DKFP binding" evidence="2">
        <location>
            <begin position="209"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="region of interest" description="DKFP binding" evidence="2">
        <location>
            <begin position="237"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="2">
        <location>
            <position position="33"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="2">
        <location>
            <position position="153"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with substrate" evidence="2">
        <location>
            <position position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF96193.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="272" mass="29465" checksum="98D6046D4A17A027" modified="2011-07-27" version="1">MDIFNNIKNIGKIIRLERIFNRTSAKTVIVPMDHGVSSGPIKGLINMKETINAVADGGANAVLLHKGIVRHGHRGYGKDVGLIIHLSAGTSLSPDPNKKVIVTSVEEAIRMGADAVSIHVNVGADTDYEMYQDLGRIAEICEYWGMPLIAMMYPRGKKIENERDPEVVAHAARLGAELGADVIKTNYTGDPDTFKEVVKGCPAPIVIAGGPKTNTDEEFLRMVKDAIDAGAVGVAIGRNIFQHENVRGITRAVSRIVHEGIDVEEALKELKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCL0</accession>
    <name>F6BCL0_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 5">30S ribosomal protein S2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">rps2</name>
        <name evidence="7" type="ordered locus">Metig_0671</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5 6">Belongs to the universal ribosomal protein uS2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96221.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798824.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0671"/>
    <dbReference type="EnsemblBacteria" id="AEF96221">
        <property type="protein sequence ID" value="AEF96221"/>
        <property type="gene ID" value="Metig_0671"/>
    </dbReference>
    <dbReference type="GeneID" id="10643511"/>
    <dbReference type="KEGG" id="mig:Metig_0671"/>
    <dbReference type="HOGENOM" id="CLU_058171_3_0_2"/>
    <dbReference type="OMA" id="QQKTKDM"/>
    <dbReference type="OrthoDB" id="63341at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01425">
        <property type="entry name" value="RPS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00291_A">
        <property type="entry name" value="Ribosomal_S2_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001865">
        <property type="entry name" value="Ribosomal_S2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023454">
        <property type="entry name" value="Ribosomal_S2_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018130">
        <property type="entry name" value="Ribosomal_S2_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005707">
        <property type="entry name" value="Ribosomal_S2_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023591">
        <property type="entry name" value="Ribosomal_S2_flav_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11489">
        <property type="entry name" value="PTHR11489"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00318">
        <property type="entry name" value="Ribosomal_S2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00395">
        <property type="entry name" value="RIBOSOMALS2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52313">
        <property type="entry name" value="SSF52313"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01012">
        <property type="entry name" value="uS2_euk_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00963">
        <property type="entry name" value="RIBOSOMAL_S2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 5 6" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00016777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEF96221.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="223" mass="25462" checksum="20A15ABA228B1CE4" modified="2011-07-27" version="1">MAENNNMLVPLDTYLASGIHIGTQQKTEDMKKFIYRVRSDGLYVLDVRKTDERLKLAAKFLAKYEPEDILAVSRRIYTMGPLEKFGQVTGIRTIPGRFVPGTLTNPAYKGFLEPEVLFLSDPRVDRQALKEAIEIGIPIVGLCDTEHLTSFIDFVIPTNNKGRKAVALIYYLMAKEYLKNRGVISSDDEVSFTYEEFLERATNVKVRIIVPQVKGKRKRKKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCM7</accession>
    <name>F6BCM7_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Beta-lactamase domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_0688</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96238.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798841.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0688"/>
    <dbReference type="EnsemblBacteria" id="AEF96238">
        <property type="protein sequence ID" value="AEF96238"/>
        <property type="gene ID" value="Metig_0688"/>
    </dbReference>
    <dbReference type="GeneID" id="10643528"/>
    <dbReference type="KEGG" id="mig:Metig_0688"/>
    <dbReference type="HOGENOM" id="CLU_009673_5_1_2"/>
    <dbReference type="OMA" id="FSAHGDY"/>
    <dbReference type="OrthoDB" id="39954at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022712">
        <property type="entry name" value="Beta_Casp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011108">
        <property type="entry name" value="RMMBL"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10996">
        <property type="entry name" value="Beta-Casp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16661">
        <property type="entry name" value="Lactamase_B_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07521">
        <property type="entry name" value="RMMBL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01027">
        <property type="entry name" value="Beta-Casp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="SSF56281"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Lactamase_B" evidence="1">
        <location>
            <begin position="14"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="domain" description="Beta-Casp" evidence="2">
        <location>
            <begin position="221"/>
            <end position="336"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF96238.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="419" mass="46883" checksum="EC95B12252C9C30B" modified="2011-07-27" version="1">MAIVRFHGGCHQIGMSCIEIDTKKSRILLDCGMDPTNNAIPKVDENVDAVIVSHAHLDHCGAIPFYKFKKIYCTTPTADLMYITWKDTLNLSKSYKEEDIQRSLNNTETLNYKEEKQITEDITLKFYNAGHILGSASIYLDIDGKKILYTGDINETPTRTLLPADTDVDEIDVLIIESTYGSPLDIKPSRKALEKQLIDEIAETIENGGKVIIPVFAIGRAQEILLIINNYIRSGKLKDVPVYVDGSLIHATGIYMSYLDWLNPKLKNMVENRVNPFGELKKADNSVFNKEPCIIISTSGMVQGGPVLQYLSLLKSPKNKLILTGYQAEGTLGRELEEGAEEITPFKNKIPVRGKVVKIEFSAHGDYNSLVRYIKKIPTPKKAFVVHGERYQTLSFAMTIWKSLKIPTFAPVNGSILPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCP2</accession>
    <name>F6BCP2_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ketol-acid reductoisomerase (NADP(+))</fullName>
            <shortName evidence="8">KARI</shortName>
            <ecNumber evidence="8">1.1.1.86</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Acetohydroxy-acid isomeroreductase</fullName>
            <shortName evidence="8">AHIR</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Alpha-keto-beta-hydroxylacyl reductoisomerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">ilvC</name>
        <name evidence="12" type="ordered locus">Metig_0703</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Involved in the biosynthesis of branched-chain amino acids (BCAA). Catalyzes an alkyl-migration followed by a ketol-acid reduction of (S)-2-acetolactate (S2AL) to yield (R)-2,3-dihydroxy-isovalerate. In the isomerase reaction, S2AL is rearranged via a Mg-dependent methyl migration to produce 3-hydroxy-3-methyl-2-ketobutyrate (HMKB). In the reductase reaction, this 2-ketoacid undergoes a metal-dependent reduction by NADPH to yield (R)-2,3-dihydroxy-isovalerate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>(2R)-2,3-dihydroxy-3-methylbutanoate + NADP(+) = (2S)-2-acetolactate + H(+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:22068"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:49072"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:58476"/>
            <dbReference type="EC" id="1.1.1.86"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>(2R,3R)-2,3-dihydroxy-3-methylpentanoate + NADP(+) = (S)-2-ethyl-2-hydroxy-3-oxobutanoate + H(+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:13493"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:49256"/>
            <dbReference type="ChEBI" id="CHEBI:49258"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.86"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="8">Binds 2 magnesium ions per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 8">Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 2/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 8">Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 2/4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 8 9">Belongs to the ketol-acid reductoisomerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.86" evidence="8"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96253.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798856.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0703"/>
    <dbReference type="EnsemblBacteria" id="AEF96253">
        <property type="protein sequence ID" value="AEF96253"/>
        <property type="gene ID" value="Metig_0703"/>
    </dbReference>
    <dbReference type="GeneID" id="10643543"/>
    <dbReference type="KEGG" id="mig:Metig_0703"/>
    <dbReference type="HOGENOM" id="CLU_033821_0_1_2"/>
    <dbReference type="OMA" id="RAMFSWL"/>
    <dbReference type="OrthoDB" id="25435at2157"/>
    <dbReference type="UniPathway" id="UPA00047">
        <property type="reaction ID" value="UER00056"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00049">
        <property type="reaction ID" value="UER00060"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004455">
        <property type="term" value="F:ketol-acid reductoisomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009097">
        <property type="term" value="P:isoleucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009099">
        <property type="term" value="P:valine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00435">
        <property type="entry name" value="IlvC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013023">
        <property type="entry name" value="KARI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000506">
        <property type="entry name" value="KARI_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013116">
        <property type="entry name" value="KARI_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014359">
        <property type="entry name" value="KARI_prok"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21371">
        <property type="entry name" value="PTHR21371"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01450">
        <property type="entry name" value="IlvC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07991">
        <property type="entry name" value="IlvN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000116">
        <property type="entry name" value="IlvC_gammaproteo"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="SSF48179"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00465">
        <property type="entry name" value="ilvC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51851">
        <property type="entry name" value="KARI_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51850">
        <property type="entry name" value="KARI_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8 9" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="7 8 9" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="12" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6 8 9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5 8 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0521">NADP</keyword>
    <keyword evidence="8 9 12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="KARI N-terminal Rossmann" evidence="10">
        <location>
            <begin position="2"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="domain" description="KARI C-terminal knotted" evidence="11">
        <location>
            <begin position="183"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="108"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="25"/>
            <end position="28"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="48"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="53"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="134"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8 9">
        <location>
            <position position="191"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8 9">
        <location>
            <position position="195"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8 9">
        <location>
            <position position="227"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8 9">
        <location>
            <position position="231"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8 9">
        <location>
            <position position="252"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010318"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51851"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AEF96253.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="331" mass="36741" checksum="736061D181DC008A" modified="2011-07-27" version="1">MVKIFYDKDATFDAVKDKTIAVIGYGSQGRAQSLNMEDSGLNVIVGLRPNGASWNKAINDGHEVMTIEEAAEKADIIHILIPDEVQPKVYNEQIKPYLKEGKTLSFSHGYNIHFGLIRPPEGVNVIMVAPKSPGAMVRKTYEEGFGVPGLVCVEKDYTGDALDIALGMAKGIGLTRAGVILTTFREETETDLFGEQVVLCGGVTELIKAAFETLVEAGYAPEMAYFETCHELKLIVDLIYQKGLQGMWNDVSNTAEYGGLTRRSRIINEQSREEMRKILKEIQTGEFAKEWALENIAGAPKLNALRRLEKEHLIEKVGKDLRKMCGLQKDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCQ1</accession>
    <name>F6BCQ1_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">NAD_synthase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0712</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96262.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798865.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0712"/>
    <dbReference type="EnsemblBacteria" id="AEF96262">
        <property type="protein sequence ID" value="AEF96262"/>
        <property type="gene ID" value="Metig_0712"/>
    </dbReference>
    <dbReference type="GeneID" id="10643552"/>
    <dbReference type="KEGG" id="mig:Metig_0712"/>
    <dbReference type="HOGENOM" id="CLU_061181_0_0_2"/>
    <dbReference type="OMA" id="DKPAQPC"/>
    <dbReference type="OrthoDB" id="56165at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016783">
        <property type="term" value="F:sulfurtransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01990">
        <property type="entry name" value="Alpha_ANH_like_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005232">
        <property type="entry name" value="LarE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022310">
        <property type="entry name" value="NAD/GMP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43169:SF2">
        <property type="entry name" value="PTHR43169:SF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02540">
        <property type="entry name" value="NAD_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006661">
        <property type="entry name" value="PP-lp_UCP006661"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00268">
        <property type="entry name" value="TIGR00268"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD_synthase" evidence="1">
        <location>
            <begin position="4"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96262.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="270" mass="31296" checksum="D806596F721D5368" modified="2011-07-27" version="1">MEELKKKLENLKKFFEGKKVIVAYSGGVDSSLVAKIASDTTETLAITIDNGFFSEDAIKKAEIRAKKYGIRHKVIKLENVKMHELEEPITQLKKNPTNRCYLCKKIMAIILINEKEKLGYDLIVDGTIYDDLFEDRPGIQAFREHGIKSPLAEFKISKKDVYELSNYLGMEIPKKETCLATRIRFNQEITEEKLKRVEMAEKFLSKYIKGTLRVRDDGGIARIEVNKDEIKKFFNEEFTKMVVDELKKLGFERVSLDLEGYKRPLKIKNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-06-02" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCQ7</accession>
    <name>F6BCQ7_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0718</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96268.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96268">
        <property type="protein sequence ID" value="AEF96268"/>
        <property type="gene ID" value="Metig_0718"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0718"/>
    <dbReference type="HOGENOM" id="CLU_1222530_0_0_2"/>
    <dbReference type="OMA" id="WCAFIIS"/>
    <dbReference type="OrthoDB" id="135245at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="24"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="57"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="108"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="132"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96268.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="226" mass="27461" checksum="A928172F0A7EAFC6" modified="2011-07-27" version="1">MWGVRMKDKLYHYFISYFLDKFKYISPILYILTIWLILKISSIIIDTFNFSLNEFVVYWWIVIILIIMTVSSFILDKIYKILKKYGVESSIDKGKFLKEKWLFYPLKWYLLWFLIFIDIYVLIYLQNEIISFIPQYWCNFIIWCAFIISIAQISPIKITFYSNGVNIGGRFYNYNQIKILDKKHKKVVFINNIPKYVFFDKNKYINYIFKLNNLENILITNESGND</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCR4</accession>
    <name>F6BCR4_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">UPF0235 protein Metig_0725</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_0725</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the UPF0235 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96275.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798878.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0725"/>
    <dbReference type="EnsemblBacteria" id="AEF96275">
        <property type="protein sequence ID" value="AEF96275"/>
        <property type="gene ID" value="Metig_0725"/>
    </dbReference>
    <dbReference type="GeneID" id="10643565"/>
    <dbReference type="KEGG" id="mig:Metig_0725"/>
    <dbReference type="HOGENOM" id="CLU_130694_6_1_2"/>
    <dbReference type="OMA" id="IGKINEW"/>
    <dbReference type="OrthoDB" id="123783at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1200.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00634">
        <property type="entry name" value="UPF0235"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005228">
        <property type="entry name" value="CHP00251"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003746">
        <property type="entry name" value="DUF167"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036591">
        <property type="entry name" value="YggU-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13420">
        <property type="entry name" value="PTHR13420"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02594">
        <property type="entry name" value="DUF167"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01152">
        <property type="entry name" value="DUF167"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69786">
        <property type="entry name" value="SSF69786"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00251">
        <property type="entry name" value="TIGR00251"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF96275.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="100" mass="11414" checksum="9FFFA997AF8616A1" modified="2011-07-27" version="1">MVEKIIKETKEGILIDIDVTPNAKKNEIGSINEWRNRLEVKIKAMAKDGKANKEIVKLFKNIFKKDVEIVSGLTSPQKTLLIKDAKKEEVIKILENLVKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCT2</accession>
    <name>F6BCT2_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glycosyl transferase family 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0745</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96293.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798896.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0745"/>
    <dbReference type="EnsemblBacteria" id="AEF96293">
        <property type="protein sequence ID" value="AEF96293"/>
        <property type="gene ID" value="Metig_0745"/>
    </dbReference>
    <dbReference type="GeneID" id="10643584"/>
    <dbReference type="KEGG" id="mig:Metig_0745"/>
    <dbReference type="HOGENOM" id="CLU_033536_7_4_2"/>
    <dbReference type="OMA" id="IYTEYSM"/>
    <dbReference type="OrthoDB" id="17570at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glyco_trans_2-like" evidence="1">
        <location>
            <begin position="8"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96293.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="230" mass="26020" checksum="4F578508D1956FCD" modified="2011-07-27" version="1">MNKDDIFVVIPAYNEEKMIGNTLRNLKKEGYKNIIVVDDGSVDKTSEIAKKEGVIVCRHILNRGLGGALGTGIKCALLYKPKIIVTFDADGQHHPKDIEKVAKPILEEGYDVVVGSRLMDKNEVKNMPLVKRIGNWGLNFITYLMGGYFTTDSQSGLRAFSYEAAKKVIKDLKSNRYEVSSEFIVLFKKHNLKFKEVPIKTIYTEYSMSRGTNVKTGFKILFKLIMQKIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCU2</accession>
    <name>F6BCU2_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_0757</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96303.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798906.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0757"/>
    <dbReference type="EnsemblBacteria" id="AEF96303">
        <property type="protein sequence ID" value="AEF96303"/>
        <property type="gene ID" value="Metig_0757"/>
    </dbReference>
    <dbReference type="GeneID" id="25395056"/>
    <dbReference type="KEGG" id="mig:Metig_0757"/>
    <dbReference type="HOGENOM" id="CLU_037290_0_0_2"/>
    <dbReference type="OMA" id="GVYFIFS"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001646">
        <property type="entry name" value="5peptide_repeat"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13576">
        <property type="entry name" value="Pentapeptide_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="350"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="375"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="425"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="515"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="217"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF96303.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="539" mass="63701" checksum="3DA4120153C06FD1" modified="2011-07-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCX9</accession>
    <name>F6BCX9_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase</fullName>
            <shortName evidence="5">HPPK</shortName>
            <ecNumber evidence="5">2.7.6.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">6-hydroxymethyl-7,8-dihydropterin diphosphokinase</fullName>
            <shortName evidence="5">6-HMPDK</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">7,8-dihydro-6-hydroxymethylpterin diphosphokinase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">7,8-dihydro-6-hydroxymethylpterin pyrophosphokinase</fullName>
            <shortName evidence="5">PPPK</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">mptE</name>
        <name evidence="7" type="ordered locus">Metig_0795</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the transfer of diphosphate from ATP to 6-hydroxymethyl-7,8-dihydropterin (6-HMD), leading to 6-hydroxymethyl-7,8-dihydropterin diphosphate (6-HMDP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>6-hydroxymethyl-7,8-dihydropterin + ATP = (7,8-dihydropterin-6-yl)methyl diphosphate + AMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11412"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:44841"/>
            <dbReference type="ChEBI" id="CHEBI:72950"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.7.6.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="5">Cofactor biosynthesis; 5,6,7,8-tetrahydromethanopterin biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the archaeal 6-HMPDK family.</text>
    </comment>
    <dbReference type="EC" id="2.7.6.3" evidence="5"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96340.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798943.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0795"/>
    <dbReference type="EnsemblBacteria" id="AEF96340">
        <property type="protein sequence ID" value="AEF96340"/>
        <property type="gene ID" value="Metig_0795"/>
    </dbReference>
    <dbReference type="GeneID" id="10643635"/>
    <dbReference type="KEGG" id="mig:Metig_0795"/>
    <dbReference type="HOGENOM" id="CLU_093043_0_0_2"/>
    <dbReference type="OMA" id="HAHGDNM"/>
    <dbReference type="OrthoDB" id="105604at2157"/>
    <dbReference type="UniPathway" id="UPA00065"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003848">
        <property type="term" value="F:2-amino-4-hydroxy-6-hydroxymethyldihydropteridine diphosphokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004788">
        <property type="term" value="F:thiamine diphosphokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001118">
        <property type="term" value="P:tetrahydromethanopterin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009229">
        <property type="term" value="P:thiamine diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02131">
        <property type="entry name" value="HMPDK_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027510">
        <property type="entry name" value="HMPDK_MptE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002826">
        <property type="entry name" value="MptE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036759">
        <property type="entry name" value="TPK_catalytic_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39648">
        <property type="entry name" value="PTHR39648"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01973">
        <property type="entry name" value="MAF_flag10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63999">
        <property type="entry name" value="SSF63999"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 5" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="MAF_flag10" evidence="6">
        <location>
            <begin position="34"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEF96340.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="241" mass="27447" checksum="6EA18F5B46ECA2B8" modified="2011-07-27" version="1">MDMETWKEFYNKIMDDFRFDKEKDVESAIILNEIIEKEKNNIDIEEVKKLISGKEVYVFGAGPSLKKHVKIIKEKNTQNPIIAADGATKALLEEGIVPNIIISDLDGDINSIIEANKKGAIVVIHAHGDNIDKIKEYARTLKNIIGSTQIPHFEQLKLSNLINYGGFTDGDRCCFLAEHLEAKKIILCGMDFGIYVTKYSRPNIKNDIEIADDIKQKKLKYAEELVHWLKIHGKSEIVYLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCZ2</accession>
    <name>F6BCZ2_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SWIM-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_0808</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96353.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798955.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0808"/>
    <dbReference type="EnsemblBacteria" id="AEF96353">
        <property type="protein sequence ID" value="AEF96353"/>
        <property type="gene ID" value="Metig_0808"/>
    </dbReference>
    <dbReference type="GeneID" id="10643649"/>
    <dbReference type="KEGG" id="mig:Metig_0808"/>
    <dbReference type="HOGENOM" id="CLU_552780_0_0_2"/>
    <dbReference type="OMA" id="NCKHGVA"/>
    <dbReference type="OrthoDB" id="100609at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007527">
        <property type="entry name" value="Znf_SWIM"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04434">
        <property type="entry name" value="SWIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50966">
        <property type="entry name" value="ZF_SWIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SWIM-type" evidence="2">
        <location>
            <begin position="45"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF96353.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="493" mass="59046" checksum="645B141D007F518B" modified="2011-07-27" version="1">MEEIIQKYGYIIYNRGVEYYKDKRVQQVLKFKNKLYGKVMGSFVYDVVVDLKSLESKCSCPYGHNCKHGVATILCYMNNEYVDVDKIVDKLKCVDKEKLLDFILEKIGRNPEFALNFVGLLDDGSELSYIYKRVKNKLLTYIAIMKSGTYIDKNTAKEIGNFLLENKNFLSKEDLIEFLEVVIKEYENYGGFYDDYTDYCYEELVLEPLGEVLFDKDLECEDLVRIFKLYDEDDYGLMEIIYDKFLKKADKFSKCVEMLKDYLDEYDYIELLIKLGKTDEALNQIHKSKLEKSINFELLTEIDENKAIDYGIKNKLYENVVMHYYGKNYYDKIVELFRKYELADYVSEIVYNSIIKSEPEDEEDLLTKLFFKTKNIVVKYNIAMKLNNKEMLLEVFNKLCEKGRYNYYDYEFLNVVNKLLLQFNEKSIINKLKDIILEHINMKTQWSYEMAVELLDMIRKSDYETFREMLDYIKKEHYRKRNLMALINKKKWF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCZ5</accession>
    <name>F6BCZ5_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Metig_0811</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96356.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0811"/>
    <dbReference type="EnsemblBacteria" id="AEF96356">
        <property type="protein sequence ID" value="AEF96356"/>
        <property type="gene ID" value="Metig_0811"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0811"/>
    <dbReference type="HOGENOM" id="CLU_3163118_0_0_2"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110001">
        <property type="term" value="C:toxin-antitoxin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004540">
        <property type="term" value="F:ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008201">
        <property type="entry name" value="HepT-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01934">
        <property type="entry name" value="DUF86"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-1277">Toxin-antitoxin system</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEF96356.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="47" mass="5645" checksum="4C916838A31DC548" modified="2011-07-27" version="1">MKKDVKIYLNHNIPWKEFAGMRDILIHKYFGVDLSLTWEVVKKDIPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>F6BD25</accession>
    <name>F6BD25_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Isoleucine--tRNA ligase</fullName>
            <ecNumber evidence="7">6.1.1.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Isoleucyl-tRNA synthetase</fullName>
            <shortName evidence="7">IleRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">ileS</name>
        <name evidence="11" type="ordered locus">Metig_0841</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile)</text>
            <dbReference type="Rhea" id="RHEA:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:9666"/>
            <dbReference type="Rhea" id="RHEA-COMP:9695"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58045"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78528"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.5"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="7">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="7">IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.5" evidence="7"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96386.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798988.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0841"/>
    <dbReference type="EnsemblBacteria" id="AEF96386">
        <property type="protein sequence ID" value="AEF96386"/>
        <property type="gene ID" value="Metig_0841"/>
    </dbReference>
    <dbReference type="GeneID" id="10643682"/>
    <dbReference type="KEGG" id="mig:Metig_0841"/>
    <dbReference type="HOGENOM" id="CLU_001493_1_1_2"/>
    <dbReference type="OMA" id="HLGTAWN"/>
    <dbReference type="OrthoDB" id="4914at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002161">
        <property type="term" value="F:aminoacyl-tRNA editing activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004822">
        <property type="term" value="F:isoleucine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006428">
        <property type="term" value="P:isoleucyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07961">
        <property type="entry name" value="Anticodon_Ia_Ile_ABEc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02003">
        <property type="entry name" value="Ile_tRNA_synth_type2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002300">
        <property type="entry name" value="aa-tRNA-synth_Ia"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033709">
        <property type="entry name" value="Anticodon_Ile_ABEc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002301">
        <property type="entry name" value="Ile-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023586">
        <property type="entry name" value="Ile-tRNA-ligase_type2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013155">
        <property type="entry name" value="M/V/L/I-tRNA-synth_anticd-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009008">
        <property type="entry name" value="Val/Leu/Ile-tRNA-synth_edit"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42780">
        <property type="entry name" value="PTHR42780"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08264">
        <property type="entry name" value="Anticodon_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00133">
        <property type="entry name" value="tRNA-synt_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00984">
        <property type="entry name" value="TRNASYNTHILE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="SSF47323"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50677">
        <property type="entry name" value="SSF50677"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00392">
        <property type="entry name" value="ileS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7 8" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="4 7 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 7 8" id="KW-0436">Ligase</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 7 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 7 8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="tRNA-synt_1" evidence="9">
        <location>
            <begin position="16"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="domain" description="Anticodon_1" evidence="10">
        <location>
            <begin position="681"/>
            <end position="825"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'HIGH' region" evidence="7">
        <location>
            <begin position="46"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'KMSKS' region" evidence="7">
        <location>
            <begin position="597"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="600"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF08264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AEF96386.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="1034" mass="121570" checksum="24BDA42AAEA28921" modified="2011-07-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>F6BD39</accession>
    <name>F6BD39_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Phosphosulfolactate synthase</fullName>
            <ecNumber evidence="2">4.4.1.19</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0856</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the phosphosulfolactate synthase family.</text>
    </comment>
    <dbReference type="EC" id="4.4.1.19" evidence="2"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96400.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799002.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0856"/>
    <dbReference type="EnsemblBacteria" id="AEF96400">
        <property type="protein sequence ID" value="AEF96400"/>
        <property type="gene ID" value="Metig_0856"/>
    </dbReference>
    <dbReference type="GeneID" id="10643697"/>
    <dbReference type="KEGG" id="mig:Metig_0856"/>
    <dbReference type="HOGENOM" id="CLU_062679_2_0_2"/>
    <dbReference type="OMA" id="MFEASNA"/>
    <dbReference type="OrthoDB" id="37705at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043817">
        <property type="term" value="F:phosphosulfolactate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019295">
        <property type="term" value="P:coenzyme M biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022370">
        <property type="entry name" value="Arch_ComA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003830">
        <property type="entry name" value="ComA_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036112">
        <property type="entry name" value="ComA_synth_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02679">
        <property type="entry name" value="ComA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102110">
        <property type="entry name" value="SSF102110"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03849">
        <property type="entry name" value="arch_ComA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96400.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="251" mass="28368" checksum="3F0D019F191B1EF2" modified="2011-07-27" version="1">MNAFSFLSKERDEGITMVLDKGLSPNFIRDYLRVCGKHIDFVKFGWGTSAVQDRDVVKEKISIYKEYGIKTYPGGTLFEAAFSKGMFDEFLEECKDLGFDGVEISNGSMDFSLEDRKYAIKRAKDEGFIVLTEVGKKSVEKDRELTIEDRIKLINFDLECGADYVIIEGRESGKCIGLFDEKGNVKKDELEILAKNVDIKKVIFEAPNKNQQVEFILKFGSGVNLGNIAYDEVISLETLRRGLRGDTFGKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>F6BD70</accession>
    <name>F6BD70_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Archaeal Rqc2 homolog aRqcH</fullName>
            <shortName evidence="1">aRqcH</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rqcH</name>
        <name evidence="3" type="ordered locus">Metig_0888</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Probably part of the ribosome quality control system (RQC). May mediate the addition of alanine residues (Ala tailing) to incompletely synthesized nascent chains from stalled ribosomes, leading to their degradation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Associates with stalled 50S ribosomal subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the NEMF family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96431.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799033.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0888"/>
    <dbReference type="EnsemblBacteria" id="AEF96431">
        <property type="protein sequence ID" value="AEF96431"/>
        <property type="gene ID" value="Metig_0888"/>
    </dbReference>
    <dbReference type="GeneID" id="10643732"/>
    <dbReference type="KEGG" id="mig:Metig_0888"/>
    <dbReference type="HOGENOM" id="CLU_003612_2_1_2"/>
    <dbReference type="OMA" id="MVYGKRN"/>
    <dbReference type="OrthoDB" id="69351at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043023">
        <property type="term" value="F:ribosomal large subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072344">
        <property type="term" value="P:rescue of stalled ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00844_A">
        <property type="entry name" value="RqcH_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008532">
        <property type="entry name" value="NFACT_RNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043681">
        <property type="entry name" value="RqcH_archaeal"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05670">
        <property type="entry name" value="NFACT-R_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="1" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="NFACT-R_1" evidence="2">
        <location>
            <begin position="485"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="307"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="426"/>
            <end position="471"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF96431.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="675" mass="78929" checksum="689D55B6D9886846" modified="2011-07-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDD0</accession>
    <name>F6BDD0_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="3">PHP domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_0948</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96491.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799093.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0948"/>
    <dbReference type="EnsemblBacteria" id="AEF96491">
        <property type="protein sequence ID" value="AEF96491"/>
        <property type="gene ID" value="Metig_0948"/>
    </dbReference>
    <dbReference type="GeneID" id="10643794"/>
    <dbReference type="KEGG" id="mig:Metig_0948"/>
    <dbReference type="HOGENOM" id="CLU_072983_1_0_2"/>
    <dbReference type="OMA" id="DIILWSY"/>
    <dbReference type="OrthoDB" id="85871at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004013">
        <property type="entry name" value="PHP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003141">
        <property type="entry name" value="Pol/His_phosphatase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016195">
        <property type="entry name" value="Pol/histidinol_Pase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02811">
        <property type="entry name" value="PHP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00481">
        <property type="entry name" value="POLIIIAc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89550">
        <property type="entry name" value="SSF89550"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="249"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="domain" description="POLIIIAc" evidence="2">
        <location>
            <begin position="3"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF96491.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="290" mass="32570" checksum="E4407E1DAA99EA1C" modified="2011-07-27" version="1">MRADLHIHSKYSGIMKYMGLKFPDSVEEPRRIMKYAKKNKIDVIAVTDHNTIRGGIETKKLEKEFGVEVIVGSEIMSKDGEIIGLFLNEEIQSGLSAEETIEKIHEQGGLAIAPHPYSPICKALNDKIFDLKLDGVEVFNAYHRDGIVNNIALEKVTKNYHKKPVAFIGSSDAHLAKMIGNGYTNFEGNCADDLYEAIIRRKTSFGGHPTPLSDIILWSYNIVYVSEKALIKSMMFKNPTLNIRFHKKLLAMFGGLIYIATPLPLIAGILGNFYLKRKAKSKLKENNLIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDD7</accession>
    <name>F6BDD7_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Shwachman-Bodian-Diamond syndrome protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metig_0955</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SDO1/SBDS family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96498.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799100.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0955"/>
    <dbReference type="EnsemblBacteria" id="AEF96498">
        <property type="protein sequence ID" value="AEF96498"/>
        <property type="gene ID" value="Metig_0955"/>
    </dbReference>
    <dbReference type="GeneID" id="10643801"/>
    <dbReference type="KEGG" id="mig:Metig_0955"/>
    <dbReference type="HOGENOM" id="CLU_043216_2_0_2"/>
    <dbReference type="OMA" id="AVNPQMD"/>
    <dbReference type="OrthoDB" id="59861at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042256">
        <property type="term" value="P:mature ribosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.900">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1250.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035647">
        <property type="entry name" value="EFG_III/V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036786">
        <property type="entry name" value="Ribosome_mat_SBDS_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018978">
        <property type="entry name" value="Ribosome_mat_Sdo1/SBDS_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019783">
        <property type="entry name" value="Ribosome_mat_Sdo1/SBDS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002140">
        <property type="entry name" value="Sdo1/SBDS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039100">
        <property type="entry name" value="Sdo1/SBDS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037188">
        <property type="entry name" value="Sdo1/SBDS_central_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10927">
        <property type="entry name" value="PTHR10927"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01172">
        <property type="entry name" value="SBDS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09377">
        <property type="entry name" value="SBDS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109728">
        <property type="entry name" value="SSF109728"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54980">
        <property type="entry name" value="SSF54980"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89895">
        <property type="entry name" value="SSF89895"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00291">
        <property type="entry name" value="RNA_SBDS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SBDS" evidence="2">
        <location>
            <begin position="7"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="domain" description="SBDS_C" evidence="3">
        <location>
            <begin position="100"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF09377"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEF96498.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="235" mass="26610" checksum="3C6057675AF9BFA4" modified="2011-07-27" version="1">MVSVDKAVIARYQSHGEKFEILVDPYLAAKLKEGQDIDISEILAIEAVYKDSGKGEKASEDALLKVFGTTDVKEIAKQIILKGHVQLTAQQRKEMQEQKKKQIISLIAKNTINPQTDTPHPPKRIEKALEEARVNIDIYKSAEEQLPRIIKELRKILPIKFEKRDVAIKIPAEFAGSAYHILHQYGTVKQEEWQSDGSLIVVIEIPSGIEAEFYAHLNKVTKGNVQTKVLKRYDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDE2</accession>
    <name>F6BDE2_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 7">Prefoldin subunit beta</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6 7">GimC subunit beta</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">pfdB</name>
        <name evidence="9" type="ordered locus">Metig_0960</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5 7">Molecular chaperone capable of stabilizing a range of proteins. Seems to fulfill an ATP-independent, HSP70-like function in archaeal de novo protein folding.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 7">Heterohexamer of two alpha and four beta subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the prefoldin subunit beta family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96503.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799105.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0960"/>
    <dbReference type="EnsemblBacteria" id="AEF96503">
        <property type="protein sequence ID" value="AEF96503"/>
        <property type="gene ID" value="Metig_0960"/>
    </dbReference>
    <dbReference type="GeneID" id="10643806"/>
    <dbReference type="KEGG" id="mig:Metig_0960"/>
    <dbReference type="HOGENOM" id="CLU_131909_0_1_2"/>
    <dbReference type="OMA" id="PPQVQAM"/>
    <dbReference type="OrthoDB" id="123614at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016272">
        <property type="term" value="C:prefoldin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00307">
        <property type="entry name" value="PfdB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002777">
        <property type="entry name" value="PFD_beta-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012713">
        <property type="entry name" value="PfdB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009053">
        <property type="entry name" value="Prefoldin"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01920">
        <property type="entry name" value="Prefoldin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02338">
        <property type="entry name" value="gimC_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0143">Chaperone</keyword>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="14"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00025077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00033461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AEF96503.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="115" mass="13288" checksum="7129DE6994E11211" modified="2011-07-27" version="1">MELPPSVQSQILQFQQLQQQLQMIVMQKQQIEVQTKEIEKALEELGKATGDEVYKMAGGIFVKRNKEDVKKELEEKLETLKLRISTLAKQEEKMQKRLSELQEKLQQAMASTAAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-06-02" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDK1</accession>
    <name>F6BDK1_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_1020</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96562.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799164.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96562">
        <property type="protein sequence ID" value="AEF96562"/>
        <property type="gene ID" value="Metig_1020"/>
    </dbReference>
    <dbReference type="GeneID" id="10643874"/>
    <dbReference type="KEGG" id="mig:Metig_1020"/>
    <dbReference type="HOGENOM" id="CLU_129114_0_0_2"/>
    <dbReference type="OMA" id="PECCIKS"/>
    <dbReference type="OrthoDB" id="90313at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007556">
        <property type="entry name" value="DUF483"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04467">
        <property type="entry name" value="DUF483"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF96562.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="182" mass="21772" checksum="8C3B7CD685F11691" modified="2011-07-27" version="1">MKEILNKIQELRKKDSSNFKILGEYIKDLDEEKYNYIIFRLKKQIEIVEKYKPKVRPAIDPMVSMELGVYRRLDDYELGKLLNYPECCIKSFAEDFRIGIDSEHLKEAEEIKEKFSNAYAIILPSGFIPCSLKCEKSWKRNLIAVVTEEEYYKILELERKLKEKLPHFHGAYDEYYEKIILK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDP2</accession>
    <name>F6BDP2_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1064</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96603.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799204.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1064"/>
    <dbReference type="EnsemblBacteria" id="AEF96603">
        <property type="protein sequence ID" value="AEF96603"/>
        <property type="gene ID" value="Metig_1064"/>
    </dbReference>
    <dbReference type="GeneID" id="10643918"/>
    <dbReference type="KEGG" id="mig:Metig_1064"/>
    <dbReference type="HOGENOM" id="CLU_099320_1_0_2"/>
    <dbReference type="OMA" id="FIYYRIV"/>
    <dbReference type="OrthoDB" id="120839at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002798">
        <property type="entry name" value="SpoIIM-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01944">
        <property type="entry name" value="SpoIIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="148"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96603.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="179" mass="20049" checksum="BC19936063362898" modified="2011-07-27" version="1">MEKLLSILTILIFSVGFTSGFIFINDFSKNYNREEINQNLSINFNFPTILLTNLKVILLMLAGAITFGLSTFINLIFNGFNVGILIGSTFLTNEPLKLITALILPHGIFEIPAMLISATAGFKIPYEVILYLLDKKEKPITEEDIKEFLKLALISIILIIIAAFVEVYLTPKIANYLLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDR4</accession>
    <name>F6BDR4_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Acetyl-CoA decarbonylase/synthase complex subunit gamma</fullName>
            <shortName evidence="5">ACDS complex subunit gamma</shortName>
            <ecNumber evidence="5">2.1.1.245</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">5-methyltetrahydrosarcinapterin:corrinoid/iron-sulfur protein Co-methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">ACDS complex methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">Corrinoid/iron-sulfur component large subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">cdhE</name>
        <name evidence="9" type="ordered locus">Metig_1086</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Part of a complex that catalyzes the reversible cleavage of acetyl-CoA, allowing autotrophic growth from CO(2).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>5,6,7,8-tetrahydrosarcinapterin + methyl-Co(III)-[corrinoid Fe-S protein] = 5-methyltetrahydrosarcinapterin + Co(I)-[corrinoid Fe-S protein] + H(+)</text>
            <dbReference type="Rhea" id="RHEA:45196"/>
            <dbReference type="Rhea" id="RHEA-COMP:11110"/>
            <dbReference type="Rhea" id="RHEA-COMP:11111"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:59924"/>
            <dbReference type="ChEBI" id="CHEBI:64267"/>
            <dbReference type="ChEBI" id="CHEBI:85033"/>
            <dbReference type="ChEBI" id="CHEBI:85035"/>
            <dbReference type="EC" id="2.1.1.245"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="5">Binds 1 [4Fe-4S] cluster.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>corrinoid</name>
            <dbReference type="ChEBI" id="CHEBI:33913"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="5">Heterodimer of delta and gamma chains. The ACDS complex is made up of alpha, epsilon, beta, gamma and delta chains with a probable stoichiometry of (alpha(2)epsilon(2))(4)-beta(8)-(gamma(1)delta(1))(8).</text>
    </comment>
    <dbReference type="EC" id="2.1.1.245" evidence="5"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96625.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799226.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1086"/>
    <dbReference type="EnsemblBacteria" id="AEF96625">
        <property type="protein sequence ID" value="AEF96625"/>
        <property type="gene ID" value="Metig_1086"/>
    </dbReference>
    <dbReference type="GeneID" id="10643940"/>
    <dbReference type="KEGG" id="mig:Metig_1086"/>
    <dbReference type="HOGENOM" id="CLU_050002_0_0_2"/>
    <dbReference type="OMA" id="SCMAFAT"/>
    <dbReference type="OrthoDB" id="31154at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046356">
        <property type="term" value="P:acetyl-CoA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01136">
        <property type="entry name" value="CdhE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007202">
        <property type="entry name" value="4Fe-4S_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016041">
        <property type="entry name" value="Ac-CoA_synth_d_su_TIM-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016218">
        <property type="entry name" value="AcylCoA_decarb/synth_gsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023427">
        <property type="entry name" value="AcylCoA_decarb/synth_gsu_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011005">
        <property type="entry name" value="Dihydropteroate_synth-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03599">
        <property type="entry name" value="CdhD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04060">
        <property type="entry name" value="FeS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000376">
        <property type="entry name" value="AcCoA_decarb_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51717">
        <property type="entry name" value="SSF51717"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51656">
        <property type="entry name" value="4FE4S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 5 7" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="5" id="KW-0170">Cobalt</keyword>
    <keyword evidence="3 5 7" id="KW-0408">Iron</keyword>
    <keyword evidence="4 5 7" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2 5 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="4Fe-4S" evidence="8">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="5-methoxybenzimidazolylcob(I)amide binding" evidence="6">
        <location>
            <begin position="402"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5 7">
        <location>
            <position position="18"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5 7">
        <location>
            <position position="21"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5 7">
        <location>
            <position position="26"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5 7">
        <location>
            <position position="43"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="372"/>
        </location>
        <ligand>
            <name>5-methoxybenzimidazolylcob(I)amide</name>
            <dbReference type="ChEBI" id="CHEBI:157765"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="378"/>
        </location>
        <ligand>
            <name>5-methoxybenzimidazolylcob(I)amide</name>
            <dbReference type="ChEBI" id="CHEBI:157765"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="465"/>
        </location>
        <ligand>
            <name>5-methoxybenzimidazolylcob(I)amide</name>
            <dbReference type="ChEBI" id="CHEBI:157765"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR000376-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000376-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AEF96625.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="493" mass="55688" checksum="2E74EB58B665DBE2" modified="2011-07-27" version="1">MAKVSAMDIYKLLPKTNCKKCGEPSCMAFATKLQNKEIDIDKCPILNTPKFEENRKKLIEMISPPVKEVWFGNDKKKCVMGGDEVMYRFELAFFNPTPIGIDIADTLPDEEIKNRAKYIEDFTFERTGEKLKLDFIAIRNASNDAEKLKNAIKIVQENTEMPIAICSLNPNNIKEALDVVKSKPLIYAATEDNLDEMIKVLKEAKKNGKNFALVLSSNNVKTLKQMAAKCLANGIEDLVFDPHTYPENIADTIDNFVMIRRSAIERGDKYLGFPILGLPINAYYYVKNKENLIESFIPDKEKAAGIYEATIANVLMNRYADALIMHGTEIWELMPVLTLRQAIYTDPRKPQAVEPGLYPIGNPDENSPVIMTTNFSLTYYTVVGDFEKDNVTCWLLVLDTGGRAVDVSVAGGQYCGENAKKLIEETKIEEKVNHRILILPALAASTRGDIEDKTGWTCVVGTRDSSQVGEFLRKNWERILNEWKEKNNLNNKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDS6</accession>
    <name>F6BDS6_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Triphosphoribosyl-dephospho-CoA protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_1098</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96637.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799238.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1098"/>
    <dbReference type="EnsemblBacteria" id="AEF96637">
        <property type="protein sequence ID" value="AEF96637"/>
        <property type="gene ID" value="Metig_1098"/>
    </dbReference>
    <dbReference type="GeneID" id="10643956"/>
    <dbReference type="KEGG" id="mig:Metig_1098"/>
    <dbReference type="HOGENOM" id="CLU_063627_0_0_2"/>
    <dbReference type="OMA" id="GNTHFGA"/>
    <dbReference type="OrthoDB" id="62406at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046917">
        <property type="term" value="F:triphosphoribosyl-dephospho-CoA synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002736">
        <property type="entry name" value="CitG"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01874">
        <property type="entry name" value="CitG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF96637.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="294" mass="33120" checksum="3CDC69625C07F570" modified="2011-07-27" version="1">MNPFDIMRASQIACCLEVSAFKPGNVHRNRDYSDIKYHHFISSGIAFGQVVYEASQSEGNIGYYIKRGVIESKKWSPTNANLGIIMLHMPIAMASGKLEEFNKDALKKNLKDIVLNTTVEDAINVYDAINIAMAGGLNKPKDGPDVASEGAKKELIEKNLTLYDVFKISSEWDNISKEWTNNFKISFEGYELINKYYNETNDIHLSITKTFLTLLSKYPDTLIARKKGIEVAKMVSKMAKDVLDNFSMEKVVEFDKYLSKEGNKLNPGTTADLIASSLMIFLLDKIDKGETILW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-06-02" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDS7</accession>
    <name>F6BDS7_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1099</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96638.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799239.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96638">
        <property type="protein sequence ID" value="AEF96638"/>
        <property type="gene ID" value="Metig_1099"/>
    </dbReference>
    <dbReference type="GeneID" id="10643957"/>
    <dbReference type="KEGG" id="mig:Metig_1099"/>
    <dbReference type="HOGENOM" id="CLU_1493009_0_0_2"/>
    <dbReference type="OMA" id="RYIVEIY"/>
    <dbReference type="OrthoDB" id="126609at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="59"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="85"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96638.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="166" mass="19702" checksum="80445978F70679A6" modified="2011-07-27" version="1">MNGIGLAIYLYAGFLSFILGLLPLYVFIKYPKLERKNKELVYSYPDNLKNVIPKAMYDMLIVFAVIPAAFITFFCYLLGKIDIFAILIAIFCYLFLCYVLCFKRYIVEIYKNGFYFNQFYTWNAFDGYEKIGNKIKLIGKKWISPNVYLRDKDGKLEEILKKYFKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDS9</accession>
    <name>F6BDS9_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">dITP/XTP pyrophosphatase</fullName>
            <ecNumber evidence="5">3.6.1.66</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Non-canonical purine NTP pyrophosphatase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">Non-standard purine NTP pyrophosphatase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">Nucleoside-triphosphate diphosphatase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">Nucleoside-triphosphate pyrophosphatase</fullName>
            <shortName evidence="5">NTPase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Metig_1101</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Pyrophosphatase that catalyzes the hydrolysis of nucleoside triphosphates to their monophosphate derivatives, with a high preference for the non-canonical purine nucleotides XTP (xanthosine triphosphate), dITP (deoxyinosine triphosphate) and ITP. Seems to function as a house-cleaning enzyme that removes non-canonical purine nucleotides from the nucleotide pool, thus preventing their incorporation into DNA/RNA and avoiding chromosomal lesions.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>H2O + ITP = diphosphate + H(+) + IMP</text>
            <dbReference type="Rhea" id="RHEA:29399"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <dbReference type="ChEBI" id="CHEBI:61402"/>
            <dbReference type="EC" id="3.6.1.66"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>H2O + XTP = diphosphate + H(+) + XMP</text>
            <dbReference type="Rhea" id="RHEA:28610"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57464"/>
            <dbReference type="ChEBI" id="CHEBI:61314"/>
            <dbReference type="EC" id="3.6.1.66"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>dITP + H2O = dIMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:28342"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61194"/>
            <dbReference type="ChEBI" id="CHEBI:61382"/>
            <dbReference type="EC" id="3.6.1.66"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="5">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5 6">Belongs to the HAM1 NTPase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.66" evidence="5"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96640.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799241.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1101"/>
    <dbReference type="EnsemblBacteria" id="AEF96640">
        <property type="protein sequence ID" value="AEF96640"/>
        <property type="gene ID" value="Metig_1101"/>
    </dbReference>
    <dbReference type="GeneID" id="10643959"/>
    <dbReference type="KEGG" id="mig:Metig_1101"/>
    <dbReference type="HOGENOM" id="CLU_082080_1_0_2"/>
    <dbReference type="OMA" id="DCFADDT"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035870">
        <property type="term" value="F:dITP diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036220">
        <property type="term" value="F:ITP diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017111">
        <property type="term" value="F:nucleoside-triphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036222">
        <property type="term" value="F:XTP diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009117">
        <property type="term" value="P:nucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009146">
        <property type="term" value="P:purine nucleoside triphosphate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00515">
        <property type="entry name" value="HAM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.950.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01405">
        <property type="entry name" value="Non_canon_purine_NTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020922">
        <property type="entry name" value="dITP/XTP_pyrophosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002637">
        <property type="entry name" value="Ham1p-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029001">
        <property type="entry name" value="ITPase-like_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11067">
        <property type="entry name" value="PTHR11067"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01725">
        <property type="entry name" value="Ham1p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52972">
        <property type="entry name" value="SSF52972"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00042">
        <property type="entry name" value="TIGR00042"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0546">Nucleotide metabolism</keyword>
    <keyword evidence="3 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Substrate binding" evidence="5">
        <location>
            <begin position="8"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="5">
        <location>
            <begin position="142"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="5">
        <location>
            <begin position="170"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="5">
        <location>
            <position position="66"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="38"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="66"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="67"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="165"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEF96640.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="186" mass="21335" checksum="E136111C5DB42C1F" modified="2011-07-27" version="1">MKIIYFATGNPNKVKEANVILKDLEDIKIEQIKMPYPELQGTLEEVAEFGAKYVYEKIKKPVIVEDSGFFVEALNGFPGTYSKYVQETIGNEGILKLLENKENRNAYFKTVIGYCDENGVKLFIGVVKGKVAEEIRSKGYGFAYDSIFIPEGEDRTFAEMTTEEKSQISHRKRAFEEFKKFLLDRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDX5</accession>
    <name>F6BDX5_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Integrase catalytic region</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1147</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96686.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96686">
        <property type="protein sequence ID" value="AEF96686"/>
        <property type="gene ID" value="Metig_1147"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_1147"/>
    <dbReference type="HOGENOM" id="CLU_079271_0_0_2"/>
    <dbReference type="OMA" id="NEERPHR"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00665">
        <property type="entry name" value="rve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="1">
        <location>
            <begin position="129"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96686.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="292" mass="34760" checksum="60149328CDAD2EA8" modified="2011-07-27" version="1">MKGAKLNDKMIRKIIRWKEKGFETSRIAKKVGVTPRRVQQIWKEYRETGEIPKIKRRGRKPKKISKGEIDLVLKAVEEYKGTSAVHLEKYIENKHNIHIPHNRIYTILKEFDLTHKRKKKRKKKPKRFSASKPNEMWQMDFKIVDVDGVRYNLLLIIDDHSRFILHAGIYEEATTDVVISALEECFEKYGTPKKILTDNGTQFVPARGGISRFQKFLMEREILHIKTSIRHPQTIGKVERINREVEYRLDKFNNLKEFVEWHNQIKPHRSLSFKTPYEVYFENTKCNKEVIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDZ5</accession>
    <name>F6BDZ5_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1168</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96706.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799306.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1168"/>
    <dbReference type="EnsemblBacteria" id="AEF96706">
        <property type="protein sequence ID" value="AEF96706"/>
        <property type="gene ID" value="Metig_1168"/>
    </dbReference>
    <dbReference type="GeneID" id="10644028"/>
    <dbReference type="KEGG" id="mig:Metig_1168"/>
    <dbReference type="HOGENOM" id="CLU_1259103_0_0_2"/>
    <dbReference type="OMA" id="YVIFQWR"/>
    <dbReference type="OrthoDB" id="109107at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="199"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96706.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="222" mass="24952" checksum="94DEA062A2C55908" modified="2011-07-27" version="1">MKKFLPIFLLLMLSPVFGEFIVVPETVPINESVYVIFKWSCEQGISNFNVTINSDAVEFENNSLYYAGVAANAEIIKLFKGKAKKLGNHTIVITMDYWKNGCHIRKKHYLNISIIPKTIIKIKKEVVEKIIVKNETINESSINNITNNKSKNESAQTTVQTINKNNSNIENITNVEISINVNNSQSQKNTESLYSLHSLYYVIGGLLVGILSGIAFMYAIKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>F6BE00</accession>
    <name>F6BE00_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_1173</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96711.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799311.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1173"/>
    <dbReference type="EnsemblBacteria" id="AEF96711">
        <property type="protein sequence ID" value="AEF96711"/>
        <property type="gene ID" value="Metig_1173"/>
    </dbReference>
    <dbReference type="GeneID" id="10644033"/>
    <dbReference type="KEGG" id="mig:Metig_1173"/>
    <dbReference type="HOGENOM" id="CLU_078976_0_0_2"/>
    <dbReference type="OMA" id="YFIPPRY"/>
    <dbReference type="OrthoDB" id="84268at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018763">
        <property type="entry name" value="DUF2334"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011330">
        <property type="entry name" value="Glyco_hydro/deAcase_b/a-brl"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10096">
        <property type="entry name" value="DUF2334"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88713">
        <property type="entry name" value="SSF88713"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF96711.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="256" mass="30322" checksum="895C1E3F81F16F66" modified="2011-07-27" version="1">MGKLLYFIFSAVILVLLIIFTFEVSNSHEIDPLGNISNNISNNTTKKPIIFIHDVSPKYFNELKDIEKIINKHNYQDRTYLFIIVNHANKYNLKKYPKFVKYLHHLENEGYHIEYHGYNHIGEEFNCNKTVAEEKLNNSFKILNDCEFNTKKIKYFIPPRYKISKDAEEIFLNKNITIIMDYYILKKENNSIKKIIITNKEYTWYLPENLVKPAEILAEIDYKSSQHQFCLSIHPKAVNYGGGLEFLDYFLNITNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2020-06-17" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>F6BE02</accession>
    <name>F6BE02_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1175</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96713.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96713">
        <property type="protein sequence ID" value="AEF96713"/>
        <property type="gene ID" value="Metig_1175"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_1175"/>
    <dbReference type="HOGENOM" id="CLU_2613654_0_0_2"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96713.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="78" mass="9193" checksum="1B0A1B976FE7B07D" modified="2011-07-27" version="1">MTINLPKVLNILMVPIIMVLNASLSTLYLLLTIWEIMGFVLEFVAKNNMTFKNVKLQVLVRLCSFWQGWDYMMKRICH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>F6BE31</accession>
    <name>F6BE31_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Radical SAM domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Metig_0005</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95567.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0005"/>
    <dbReference type="EnsemblBacteria" id="AEF95567">
        <property type="protein sequence ID" value="AEF95567"/>
        <property type="gene ID" value="Metig_0005"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0005"/>
    <dbReference type="HOGENOM" id="CLU_061501_0_0_2"/>
    <dbReference type="OMA" id="CIFCYSN"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaB/NifB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Elp3" evidence="5">
        <location>
            <begin position="80"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEF95567.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="326" mass="37257" checksum="0AEE8B9821E604C9" modified="2011-07-27" version="1">MEKCSNINTTINNLFKQKNLIKYLEKSIKTKHLVEGEILEKIILRSEICDKLEEIVKNLKVVKHCIGCEGLDLNNKDPHHHPTIEITQKCNHNCVFCYSKLTNIKPGIYGNLDNCIAVTISQYGEPLLYPEKVKKGIEYVKSLGLRCDLQTNGVFLNGNLIKEFKDLGLDLVMISLNASKPETHKKLTNVDTFERVLKNIELSAKHLHTIVRAIYIPNFNDQELVELAKKLNDIGVDEIMVHQLIVHEQNKRELEKISSLENVGRIKDLLLLVDKMKENAPDVNVTIKGCLLVQLKKMDGFILNGITSDCFSEIPIIKREYHPLPW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>F6BE37</accession>
    <name>F6BE37_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">ThiamineS protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_0011</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95573.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798183.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0011"/>
    <dbReference type="EnsemblBacteria" id="AEF95573">
        <property type="protein sequence ID" value="AEF95573"/>
        <property type="gene ID" value="Metig_0011"/>
    </dbReference>
    <dbReference type="GeneID" id="10642846"/>
    <dbReference type="KEGG" id="mig:Metig_0011"/>
    <dbReference type="HOGENOM" id="CLU_114601_4_0_2"/>
    <dbReference type="OMA" id="FEIMGSD"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016155">
        <property type="entry name" value="Mopterin_synth/thiamin_S_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003749">
        <property type="entry name" value="ThiS/MoaD-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02597">
        <property type="entry name" value="ThiS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54285">
        <property type="entry name" value="SSF54285"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF95573.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="84" mass="9503" checksum="D48D6885C97B910C" modified="2011-07-27" version="1">MELVIRLFAKYRELYGEKIKITIDKNELTLKELLNILNENSINIKDDFYQGKAIISVNCDVVNSDDVVIKASDEIVIYPPVSGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>F6BE45</accession>
    <name>F6BE45_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">4Fe-4S ferredoxin iron-sulfur binding domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0019</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95581.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798191.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0019"/>
    <dbReference type="EnsemblBacteria" id="AEF95581">
        <property type="protein sequence ID" value="AEF95581"/>
        <property type="gene ID" value="Metig_0019"/>
    </dbReference>
    <dbReference type="GeneID" id="10642854"/>
    <dbReference type="KEGG" id="mig:Metig_0019"/>
    <dbReference type="HOGENOM" id="CLU_139698_11_4_2"/>
    <dbReference type="OMA" id="CISCRIC"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00037">
        <property type="entry name" value="Fer4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="28"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF95581.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="55" mass="6029" checksum="D1289E8BAFA21C40" modified="2011-07-27" version="1">MKVNYERCGYCGACVGVCDKMAIELVENLIIINNEKCTNCKLCMIVCPLNALVDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F6BE57</accession>
    <name>F6BE57_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Transcription factor CBF/NF-Y/histone domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Metig_0032</name>
        <name evidence="9" type="ordered locus">Metig_0641</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="8 10" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Binds and compact DNA (95 to 150 base pairs) to form nucleosome-like structures that contain positive DNA supercoils. Increases the resistance of DNA to thermal denaturation (in vitro).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the archaeal histone HMF family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95593.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96191.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007044378.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0032"/>
    <dbReference type="EnsemblBacteria" id="AEF95593">
        <property type="protein sequence ID" value="AEF95593"/>
        <property type="gene ID" value="Metig_0032"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96191">
        <property type="protein sequence ID" value="AEF96191"/>
        <property type="gene ID" value="Metig_0641"/>
    </dbReference>
    <dbReference type="GeneID" id="10643481"/>
    <dbReference type="KEGG" id="mig:Metig_0032"/>
    <dbReference type="KEGG" id="mig:Metig_0641"/>
    <dbReference type="HOGENOM" id="CLU_192667_0_0_2"/>
    <dbReference type="OMA" id="CKHAGRK"/>
    <dbReference type="OrthoDB" id="127272at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003958">
        <property type="entry name" value="CBFA_NFYB_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00808">
        <property type="entry name" value="CBFD_NFYB_HMF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="SSF47113"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0158">Chromosome</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBFD_NFYB_HMF" evidence="7">
        <location>
            <begin position="2"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEF95593.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AEF96191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="66" mass="7121" checksum="C96A525761F5B4A9" modified="2011-07-27" version="1">MAELPVAPMERILKKAGAERVSRAAAVKLAEALEDIAMDIAKEAVALAKHAKRKTVKVEDIKLALK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>F6BE64</accession>
    <name>F6BE64_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Coenzyme F420 hydrogenase, subunit beta</fullName>
            <ecNumber evidence="5">1.12.98.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Metig_1203</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.12.98.1" evidence="5"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96741.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799340.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1203"/>
    <dbReference type="EnsemblBacteria" id="AEF96741">
        <property type="protein sequence ID" value="AEF96741"/>
        <property type="gene ID" value="Metig_1203"/>
    </dbReference>
    <dbReference type="GeneID" id="10644063"/>
    <dbReference type="KEGG" id="mig:Metig_1203"/>
    <dbReference type="HOGENOM" id="CLU_037958_0_0_2"/>
    <dbReference type="OMA" id="DTHMYEQ"/>
    <dbReference type="OrthoDB" id="49654at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050454">
        <property type="term" value="F:coenzyme F420 hydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016151">
        <property type="term" value="F:nickel cation binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007516">
        <property type="entry name" value="Co_F420_Hydgase/DH_bsu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045220">
        <property type="entry name" value="FRHB/FDHB/HCAR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017679">
        <property type="entry name" value="FrhB_archaea"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007525">
        <property type="entry name" value="FrhB_FdhB_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31332">
        <property type="entry name" value="PTHR31332"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04432">
        <property type="entry name" value="FrhB_FdhB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04422">
        <property type="entry name" value="FrhB_FdhB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03289">
        <property type="entry name" value="frhB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0408">Iron</keyword>
    <keyword evidence="2" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FrhB_FdhB_N" evidence="3">
        <location>
            <begin position="10"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="domain" description="FrhB_FdhB_C" evidence="4">
        <location>
            <begin position="95"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF04432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEF96741.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="282" mass="31206" checksum="AAB2D2E0D7235B3A" modified="2011-07-27" version="1">MDAFGKYKTVVSARATDKKILKKSQDGGIVSAAFIYGLENGLLDGVIVADNAGEFKAVPKVATTPEEVLEAAGTKYTVCPNISVLKSAVREYALEKVGIVGTPCQIRAVRKLMKYPVGFRHTDSKIALTIGIFCMENFPYMGLKTIVEEHCGVKMEDVVKMDIGKGKFWVYTKWGETKAIKLKETHPYEQIACHVCTDYTAELADISTGSVGSPDGWSTVFVRTIRGEEFFNKMVEDGYLEIKPIEEVKPGLGLVEKLALTKKEKNTKEIEHRKELGLPVPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>F6BE83</accession>
    <name>F6BE83_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">50S ribosomal protein L2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">rpl2</name>
        <name evidence="7" type="ordered locus">Metig_1223</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">One of the primary rRNA binding proteins. Required for association of the 30S and 50S subunits to form the 70S ribosome, for tRNA binding and peptide bond formation. It has been suggested to have peptidyltransferase activity; this is somewhat controversial. Makes several contacts with the 16S rRNA in the 70S ribosome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Part of the 50S ribosomal subunit. Forms a bridge to the 30S subunit in the 70S ribosome.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the universal ribosomal protein uL2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96760.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799359.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1223"/>
    <dbReference type="EnsemblBacteria" id="AEF96760">
        <property type="protein sequence ID" value="AEF96760"/>
        <property type="gene ID" value="Metig_1223"/>
    </dbReference>
    <dbReference type="GeneID" id="10644084"/>
    <dbReference type="KEGG" id="mig:Metig_1223"/>
    <dbReference type="HOGENOM" id="CLU_036235_0_3_2"/>
    <dbReference type="OMA" id="SCIELRP"/>
    <dbReference type="OrthoDB" id="74812at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015934">
        <property type="term" value="C:large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.950.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01320_A">
        <property type="entry name" value="Ribosomal_L2_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022666">
        <property type="entry name" value="Rbsml_prot_L2_RNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_L2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002171">
        <property type="entry name" value="Ribosomal_L2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023672">
        <property type="entry name" value="Ribosomal_L2_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022669">
        <property type="entry name" value="Ribosomal_L2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022671">
        <property type="entry name" value="Ribosomal_L2_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014726">
        <property type="entry name" value="Ribosomal_L2_dom3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13691">
        <property type="entry name" value="PTHR13691"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00181">
        <property type="entry name" value="Ribosomal_L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03947">
        <property type="entry name" value="Ribosomal_L2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002158">
        <property type="entry name" value="Ribosomal_L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01383">
        <property type="entry name" value="Ribosomal_L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01382">
        <property type="entry name" value="Ribosomal_L2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="SSF50104"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00467">
        <property type="entry name" value="RIBOSOMAL_L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 7" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="4" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="Ribosomal_L2_C" evidence="6">
        <location>
            <begin position="92"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="201"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM01382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEF96760.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="241" mass="25995" checksum="090F199523762D68" modified="2011-07-27" version="1">MGKRLISQNRGRGGPNYRCPSHKRRGEVKYRKFDDLEKKGKIIGTITDILHDPGRSAPVATVKFENGEEKLLLVPEGMKVGDTIECGVAAEIKPGNILPLGEIPEGIPVFNIETVPGDGGKLVRAGGCYAHIIAHDVGKTIVKLPSGQLKALHPMCRATIGVVAGGGRKEKPFVKAGKKYHAMKAKAIKWPRVRGVAMNAVDHPFGGGRHQHTGKPTTVSRRMPPGRKVGHIAARRTGVRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-06-02" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEB3</accession>
    <name>F6BEB3_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_1253</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96790.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799389.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96790">
        <property type="protein sequence ID" value="AEF96790"/>
        <property type="gene ID" value="Metig_1253"/>
    </dbReference>
    <dbReference type="GeneID" id="10644116"/>
    <dbReference type="KEGG" id="mig:Metig_1253"/>
    <dbReference type="HOGENOM" id="CLU_551679_0_0_2"/>
    <dbReference type="OMA" id="IDMTPGR"/>
    <dbReference type="OrthoDB" id="33593at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF96790.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="497" mass="58263" checksum="F8607B0CDA7CDBAD" modified="2011-07-27" version="1">MRVWISTVGSSPLAVFNTLWMAIVKEGYVPDKVYLLWNYKVEKNKEIIKNYIKSLNESYNFNIDVDESYKVSEDDFKDFSNALKEIIVKEKEKGNEIAIDMTPGRKFMSALSMFAGIEGVSDGKNKYKCDRVYYLHLKDMGYINYPLFLIPFSIQELHEFISELSGSKKDDRKVLKKEELEDLIINRREILAILNQYFLFGKSNFEIKVENQKFATISLYENDNKAKVEINKDLKLSYYVGDYESISTILNASGLKEVKFIVYGDEKEEFSEREFYEWLFKKLKEKETYYITFDTNALINQIPRKIIDYLDKQREKISPNFAISQCVINELTNERGKIKDCEDRDFINQPSSVDRIFKLGLSQIKLLIGRNAVIIPGKGVWDNNIKESFVKFSDEHKGKLLILTSDKNFTHNLQYLPDIIPIYVDFGELKKLVKWEEIRDLIYISSIVFGKIYIKPISQVLGIWRGKNPIDWDEEKVLFRKINVMCRQAANVLRNIH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEC3</accession>
    <name>F6BEC3_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">UspA domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1263</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96800.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1263"/>
    <dbReference type="EnsemblBacteria" id="AEF96800">
        <property type="protein sequence ID" value="AEF96800"/>
        <property type="gene ID" value="Metig_1263"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_1263"/>
    <dbReference type="HOGENOM" id="CLU_049301_11_0_2"/>
    <dbReference type="OMA" id="VSHKVSQ"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006015">
        <property type="entry name" value="Universal_stress_UspA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006016">
        <property type="entry name" value="UspA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00582">
        <property type="entry name" value="Usp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006276">
        <property type="entry name" value="UspA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01438">
        <property type="entry name" value="UNVRSLSTRESS"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Usp" evidence="1">
        <location>
            <begin position="3"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96800.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="151" mass="16783" checksum="2A5214A3CA977552" modified="2011-07-27" version="1">MLYKKIVVPTDGSDVSIEAAKHAIEIGKLMKAEIYVIYVVDIAPFIGLPTEGLWETMKEILNEEGEKALKKIKKMAEEHGVNVKPEILEGVPANEIVEFAEKKRADLIVMGTSGKTGLDRFLLGSVAEKVIRNAHCPVLVVKKQKKEEKEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>F6BED6</accession>
    <name>F6BED6_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized conserved protein UCP006598</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_0036</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95597.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798206.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0036"/>
    <dbReference type="EnsemblBacteria" id="AEF95597">
        <property type="protein sequence ID" value="AEF95597"/>
        <property type="gene ID" value="Metig_0036"/>
    </dbReference>
    <dbReference type="GeneID" id="10642871"/>
    <dbReference type="KEGG" id="mig:Metig_0036"/>
    <dbReference type="HOGENOM" id="CLU_038447_0_0_2"/>
    <dbReference type="OMA" id="TTTICGD"/>
    <dbReference type="OrthoDB" id="49106at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012032">
        <property type="entry name" value="UCP006598"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09890">
        <property type="entry name" value="DUF2117"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006598">
        <property type="entry name" value="UCP006598"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF95597.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="370" mass="41045" checksum="03E209CF1D219BDE" modified="2011-07-27" version="1">MRIGVVVHGPEIVDSGYAKKIIDLLKNFGDVKAKLGGTMGRVAVIDNNLQDIIDISEKLVPSQSLKKLADNDILVLMNYGKSKETGHTFGRIVVGRANISKPIIQIERPGEKDGTIIVWNNNNSEEVKKIVDFLSKTLNLKVENCISDGLSVWKEGKRLFRKVHGVDKGEAIMVNGIVVGRAKGKEVVIVAEDGKIVDIINGEMKEHGVEKLGRVDLEKAIIKTGLLRRHPTNPNVRVLENKRGDVVFINHAGEDVLEKIKNKEISAVITIGDDTTMICGDILARFGIRIIGITDGDRDEILKSPVIAEGSAIFLIENMRDDDVGELLMKEFEKREIRGITFNECLKIVEGILNENKVRYSLQKIEKCIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEI8</accession>
    <name>F6BEI8_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_0088</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95649.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798258.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0088"/>
    <dbReference type="EnsemblBacteria" id="AEF95649">
        <property type="protein sequence ID" value="AEF95649"/>
        <property type="gene ID" value="Metig_0088"/>
    </dbReference>
    <dbReference type="GeneID" id="10642923"/>
    <dbReference type="KEGG" id="mig:Metig_0088"/>
    <dbReference type="HOGENOM" id="CLU_077076_0_0_2"/>
    <dbReference type="OMA" id="HHSYPCT"/>
    <dbReference type="OrthoDB" id="56837at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003745">
        <property type="entry name" value="DUF166"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02593">
        <property type="entry name" value="DUF166"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF95649.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="260" mass="30483" checksum="F2BBD734943A65E5" modified="2011-07-27" version="1">MKAVFIYHEGNNRMEKFYKNLLNEPDFCKICEDCYNCRGDWSFKDDVKNVVIKETKEEFIDDPYEYLPELPEGDVCIAQLHEDLLYELPLLLKEKNYKALIVPSETPHDLSPALRRDLKRMCKDYNIEFENPKPFCSLEKKERNETINKFIDYFRIGKPELEIEVERHTIKDVKVKISAPCGETYYIAKRIKNKEIKGLKEEIANAHHNYPCLGSMEYDKELGDTILHEAGYIAIESIEKALNKYKCENKFCSGNCGKLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEJ8</accession>
    <name>F6BEJ8_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Cupin 2 conserved barrel domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0098</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95659.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798268.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0098"/>
    <dbReference type="EnsemblBacteria" id="AEF95659">
        <property type="protein sequence ID" value="AEF95659"/>
        <property type="gene ID" value="Metig_0098"/>
    </dbReference>
    <dbReference type="GeneID" id="10642933"/>
    <dbReference type="KEGG" id="mig:Metig_0098"/>
    <dbReference type="HOGENOM" id="CLU_2115513_0_0_2"/>
    <dbReference type="OMA" id="TEHVQIN"/>
    <dbReference type="OrthoDB" id="109106at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006045">
        <property type="entry name" value="Cupin_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00190">
        <property type="entry name" value="Cupin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="SSF51182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cupin type-1" evidence="1">
        <location>
            <begin position="19"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF95659.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="116" mass="13446" checksum="090F269D697325A2" modified="2011-07-27" version="1">MIEKVYEFKKDATTKVVEKIVNTEHVQINHIVLPKGEQMPKHYSNSYVHLIIVKGEMTLTLEDQEPHNYKEGTIVYVPFNVKMLIQNLNSDVLEFFVIKAPHPKKLNAPEEAIKCE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEK2</accession>
    <name>F6BEK2_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Putative transcriptional regulator, CopG family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Metig_0102</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the transcriptional regulatory CopG/NikR family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95663.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798272.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF95663">
        <property type="protein sequence ID" value="AEF95663"/>
        <property type="gene ID" value="Metig_0102"/>
    </dbReference>
    <dbReference type="GeneID" id="10642937"/>
    <dbReference type="KEGG" id="mig:Metig_0102"/>
    <dbReference type="HOGENOM" id="CLU_113319_1_2_2"/>
    <dbReference type="OMA" id="IVESKNY"/>
    <dbReference type="OrthoDB" id="90041at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1220.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027271">
        <property type="entry name" value="Acetolactate_synth/TF_NikR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013321">
        <property type="entry name" value="Arc_rbn_hlx_hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002145">
        <property type="entry name" value="CopG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010985">
        <property type="entry name" value="Ribbon_hlx_hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014864">
        <property type="entry name" value="TF_NikR_Ni-bd_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08753">
        <property type="entry name" value="NikR_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01402">
        <property type="entry name" value="RHH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47598">
        <property type="entry name" value="SSF47598"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="SSF55021"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="RHH_1" evidence="5">
        <location>
            <begin position="6"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="domain" description="NikR_C" evidence="6">
        <location>
            <begin position="57"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF08753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEF95663.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="135" mass="15534" checksum="E15A1B380FC0C227" modified="2011-07-27" version="1">MVNVERISISFSKYLLKEIDNVVKKKGYSSRSELIRDAVRKHVLESNSLDKEGNISGIIIVVYTPTKESMEKMSRLYFEYNEVVKSINQSYITTSCGKNKKVEIFVVDGDAKKISEFYDEISKIDGKIYDKVIVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEK5</accession>
    <name>F6BEK5_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Peptidase M50</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0105</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95666.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798275.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0105"/>
    <dbReference type="EnsemblBacteria" id="AEF95666">
        <property type="protein sequence ID" value="AEF95666"/>
        <property type="gene ID" value="Metig_0105"/>
    </dbReference>
    <dbReference type="GeneID" id="10642940"/>
    <dbReference type="KEGG" id="mig:Metig_0105"/>
    <dbReference type="HOGENOM" id="CLU_099718_0_0_2"/>
    <dbReference type="OMA" id="SEFRAWY"/>
    <dbReference type="OrthoDB" id="118207at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="14"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="38"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="78"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="116"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="143"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="168"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF95666.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="190" mass="21620" checksum="285E3AEF7E805073" modified="2011-07-27" version="1">MSMDNLFRFSKKELIDLVVSTFAIAIIFAWPRGFSFDLWFFILLIIYLFTVGTGFILHELAHRTVARHFGAWSEFRAWYEGLALGLILKIILGFTFIAPGAVYIYKDYLTTEENGIIALAGPLTNIALAFLFLILNIPIISDIGYYVNLFLAAFNMLPIPPFDGSKVIHWNILVWAIVAIPLFLWAFGLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEK7</accession>
    <name>F6BEK7_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Chorismate mutase</fullName>
            <ecNumber evidence="2">5.4.99.5</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0107</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EC" id="5.4.99.5" evidence="2"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95668.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798277.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0107"/>
    <dbReference type="EnsemblBacteria" id="AEF95668">
        <property type="protein sequence ID" value="AEF95668"/>
        <property type="gene ID" value="Metig_0107"/>
    </dbReference>
    <dbReference type="GeneID" id="10642942"/>
    <dbReference type="KEGG" id="mig:Metig_0107"/>
    <dbReference type="HOGENOM" id="CLU_036672_1_0_2"/>
    <dbReference type="OMA" id="TDGLGKW"/>
    <dbReference type="OrthoDB" id="117835at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004106">
        <property type="term" value="F:chorismate mutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008977">
        <property type="term" value="F:prephenate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004665">
        <property type="term" value="F:prephenate dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006571">
        <property type="term" value="P:tyrosine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.380">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005121">
        <property type="entry name" value="Fdx_antiC-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036690">
        <property type="entry name" value="Fdx_antiC-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008299">
        <property type="entry name" value="Prep_DH/arog_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003099">
        <property type="entry name" value="Prephen_DH"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03147">
        <property type="entry name" value="FDX-ACB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02153">
        <property type="entry name" value="PDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006549">
        <property type="entry name" value="PDH_arog_dh_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00896">
        <property type="entry name" value="FDX-ACB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="SSF48179"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54991">
        <property type="entry name" value="SSF54991"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51176">
        <property type="entry name" value="PDH_ADH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0413">Isomerase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Prephenate/arogenate dehydrogenase" evidence="1">
        <location>
            <begin position="4"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF95668.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="442" mass="50744" checksum="E7970B6C0EE2D3CA" modified="2011-07-27" version="1">MKNYKISIIGGTDGLGKWFAKYLRNKGFDVTVTGRDIAKGKSVEKELGVKFTNNNIEAAKEGDIVIIAVPINVTERVIKEVAPHVREGCLLMDITSIKEIPSKTMEEYAKEGVTVIPTHPMFGPSTPSLMRQVVILTPSEKHMKSEWFRKVKDFLEKEGARVIIIKPEEHDRIMGVVQGLTHFAYISLGATLKELGVNIKESRKYASPIYELMIYIIGRIIGQNPYLYADIQMHNPQIKHIHETFISQCNLIKSIVEKKDREGFVKLMKEAAKHFGNEAKRGVSYSDKAVFALTKEMEKLSKMKGKEVGLKHIYSGNVHYGILKDVDGDYIIIEKNGKEIKLSTSNVMVMEEEELKKWKNENLPKYYRDISILFKKEVNENIILDLLKNKFDIEIIDVYEGEKIEEGFKSITFRIYAFENVEEKEKEFLKIIENIGGRRRFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEL6</accession>
    <name>F6BEL6_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Phosphonopyruvate decarboxylase-related protein</fullName>
            <ecNumber evidence="6">5.4.2.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Metig_1276</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the interconversion of 2-phosphoglycerate and 3-phosphoglycerate.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Carbohydrate degradation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the BPG-independent phosphoglycerate mutase family. A-PGAM subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.4.2.1" evidence="6"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96813.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799411.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1276"/>
    <dbReference type="EnsemblBacteria" id="AEF96813">
        <property type="protein sequence ID" value="AEF96813"/>
        <property type="gene ID" value="Metig_1276"/>
    </dbReference>
    <dbReference type="GeneID" id="10644139"/>
    <dbReference type="KEGG" id="mig:Metig_1276"/>
    <dbReference type="HOGENOM" id="CLU_034906_1_0_2"/>
    <dbReference type="OMA" id="HIHTKEP"/>
    <dbReference type="OrthoDB" id="17268at2157"/>
    <dbReference type="UniPathway" id="UPA00232"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046537">
        <property type="term" value="F:2,3-bisphosphoglycerate-independent phosphoglycerate mutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16011">
        <property type="entry name" value="iPGM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006124">
        <property type="entry name" value="Metalloenzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004456">
        <property type="entry name" value="Pglycerate_mutase_ApgM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042253">
        <property type="entry name" value="Pglycerate_mutase_ApgM_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31209">
        <property type="entry name" value="PTHR31209"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01676">
        <property type="entry name" value="Metalloenzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10143">
        <property type="entry name" value="PhosphMutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006392">
        <property type="entry name" value="IPGAM_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="SSF53649"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00306">
        <property type="entry name" value="apgM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="6" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metalloenzyme" evidence="5">
        <location>
            <begin position="1"/>
            <end position="396"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEF96813.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="428" mass="48685" checksum="71C47AF98F1AD5FA" modified="2011-07-27" version="1">MRAIVVLLDGLGDRPSEILGNKTPLEAAKTPNLDEFAKRGITGLMVPYKEGVPLGSEVSHLLLWGYSLNDFPGRGLIEALGEGVEVKDNEIYLRATFGFVEKHNGELLIKDRRTKDITKEEIEELIDSLPTYVNGYEFELKYTHDAHCILIIREENGWISDKISDGDPFYMNRHVLRICPIKKLCKSRMEYNKAKSTAEALNEYLKKCFKELDNHEINRKRAKMGKQKANMLLTKWAGKYKKVEPFSERWGMRGVVIGNSAVFCGLAKFLGLDYIKCGDFKKAVEMAVDLDYDFIHIHTKEIDEAAHTKNPELKKKVIEKIDACLEPLLNLEDDLIIITGDHSTPSVGNLIHSGESVPITIVGKNVRRDDVKEFNERACAKGSLYIRACDLMNIILNYTDRALLYGLRPNRILKYIPDDSKTRHLKID</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEM4</accession>
    <name>F6BEM4_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Hydro-lyase, Fe-S type, tartrate/fumarate subfamily, alpha subunit</fullName>
            <ecNumber evidence="8">4.2.1.2</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Metig_1284</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-I fumarase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.2" evidence="8"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96821.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799418.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1284"/>
    <dbReference type="EnsemblBacteria" id="AEF96821">
        <property type="protein sequence ID" value="AEF96821"/>
        <property type="gene ID" value="Metig_1284"/>
    </dbReference>
    <dbReference type="GeneID" id="10644147"/>
    <dbReference type="KEGG" id="mig:Metig_1284"/>
    <dbReference type="HOGENOM" id="CLU_041245_0_0_2"/>
    <dbReference type="OMA" id="IETYPTH"/>
    <dbReference type="OrthoDB" id="59419at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004333">
        <property type="term" value="F:fumarate hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008152">
        <property type="term" value="P:metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004646">
        <property type="entry name" value="Fe-S_hydro-lyase_TtdA-typ_cat"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05681">
        <property type="entry name" value="Fumerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00722">
        <property type="entry name" value="ttdA_fumA_fumB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="6 8" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fumerase" evidence="7">
        <location>
            <begin position="5"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF05681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEF96821.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="280" mass="30420" checksum="6945254D2EA38A5A" modified="2011-07-27" version="1">MRISDVVVELFREAVIYLPDDVKKAIEDAYGIEEGASKNILEAIIENNKIAEEKQIPLCQDTGVPIVFLKIGKNINSSEIIKIIEEIKKGVKKATEEIPLRPNVVHPLTRENFKTNVGLNAPFINIEFDENLNREIEITVFPKGAGSENMSALKMLTPAEGIEGIKKFVLETVANAGGKPCPPIVVGVGIGGTADVALKLAKKALLRKIGERHKDENIAKLEKELLEGINNLGIGAMGLGGKITALDVFIEISGCHTASLPVGICIQCWAQRKATKRIKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F6BET0</accession>
    <name>F6BET0_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CRISPR system Cms endoribonuclease Csm3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">CRISPR type III A-associated RAMP protein Csm3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Metig_1342</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CRISPR-associated Csm3 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96877.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799474.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1342"/>
    <dbReference type="EnsemblBacteria" id="AEF96877">
        <property type="protein sequence ID" value="AEF96877"/>
        <property type="gene ID" value="Metig_1342"/>
    </dbReference>
    <dbReference type="GeneID" id="10644205"/>
    <dbReference type="KEGG" id="mig:Metig_1342"/>
    <dbReference type="HOGENOM" id="CLU_067743_0_0_2"/>
    <dbReference type="OMA" id="NPVIKDP"/>
    <dbReference type="OrthoDB" id="98478at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013412">
        <property type="entry name" value="CRISPR-assoc_RAMP_Csm3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005537">
        <property type="entry name" value="RAMP_III_fam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03787">
        <property type="entry name" value="RAMPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02582">
        <property type="entry name" value="cas7_TM1809"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0051">Antiviral defense</keyword>
    <keyword evidence="4" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00033183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEF96877.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="271" mass="30941" checksum="DAA231CB58AA8AE9" modified="2011-07-27" version="1">MNKESYDKNRAFYGKIILKGNIKAVTGLHIGAQREVSEIGGIDNPVIKDPVTGLPYIPGSSLKGRLRSLLEIAVNSKQPKEKQGTGEEKFFNTKVHNIWIHVCKKYDHAIECEVCRLFGASGEENFPSRVIVRDSQLTEEWKKRFESGEVFTEAKVETAIDRITSAASPRQSERVPAGVEFEFEIIYNIEDENWKDDLKNLLSAMKMLEDSYLGGSGSRGYGKIKFKLKNPIFRSREFYFGKKEEEEIELDTEDIKELLDKFEEVFGKITI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEV9</accession>
    <name>F6BEV9_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="3">GTP-binding protein HSR1-related protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_0135</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95695.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798304.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0135"/>
    <dbReference type="EnsemblBacteria" id="AEF95695">
        <property type="protein sequence ID" value="AEF95695"/>
        <property type="gene ID" value="Metig_0135"/>
    </dbReference>
    <dbReference type="GeneID" id="10642970"/>
    <dbReference type="KEGG" id="mig:Metig_0135"/>
    <dbReference type="HOGENOM" id="CLU_011106_1_2_2"/>
    <dbReference type="OMA" id="EWQTGRF"/>
    <dbReference type="OrthoDB" id="36461at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1580.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030378">
        <property type="entry name" value="G_CP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023179">
        <property type="entry name" value="GTP-bd_ortho_bundle_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51721">
        <property type="entry name" value="G_CP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CP-type G" evidence="2">
        <location>
            <begin position="9"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="29"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF95695.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="365" mass="41962" checksum="256927A1C8EDFF7C" modified="2011-07-27" version="1">MRYKKVPVKKIVNKIINECDVILLVLDARDPETTRNKELEEKIKKMNKELIYVLNKADLVPKEVLEKWKEKLGNAVFMSAKYRMGTKILRDKIKEYLKKNNIKEGKVGVVGYPNVGKSSIINALTGRRSALTGSLAGLTKGEQWIRLTKNIKLLDTPGVLEMKDEDELIISGALRLEKAENLISPALKILKRLHDFDKTIIPNYYGVEVDEINEELLKKIGKKLKYLKKGNEVDLDRTAKSIIKDFQDGKLNYYNVEIKKYGQERQRNIEFITRYLKDFPFIDDAKMIINHLSDFDDLKRLKIKPVLGEETINGTTLVISFGEKTVDAGRKKVEEYAKEHNIELYSKFGDKIGRNRVFVGVGTKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEW9</accession>
    <name>F6BEW9_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Nuclease (SNase domain-containing protein)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0145</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95705.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798314.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0145"/>
    <dbReference type="EnsemblBacteria" id="AEF95705">
        <property type="protein sequence ID" value="AEF95705"/>
        <property type="gene ID" value="Metig_0145"/>
    </dbReference>
    <dbReference type="GeneID" id="10642980"/>
    <dbReference type="KEGG" id="mig:Metig_0145"/>
    <dbReference type="HOGENOM" id="CLU_046484_5_3_2"/>
    <dbReference type="OMA" id="DTPETYQ"/>
    <dbReference type="OrthoDB" id="92842at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004518">
        <property type="term" value="F:nuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035437">
        <property type="entry name" value="SNase_OB-fold_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016071">
        <property type="entry name" value="Staphylococal_nuclease_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002071">
        <property type="entry name" value="Thermonucl_AS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00565">
        <property type="entry name" value="SNase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00318">
        <property type="entry name" value="SNc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50199">
        <property type="entry name" value="SSF50199"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01123">
        <property type="entry name" value="TNASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01284">
        <property type="entry name" value="TNASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50830">
        <property type="entry name" value="TNASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TNase-like" evidence="1">
        <location>
            <begin position="41"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50830"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF95705.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="203" mass="23944" checksum="84F28E9225217F75" modified="2011-07-27" version="1">MESKISLTILLFFIFLSGCIEFNNHNSNGFNDNFDFIQTHEHFYGKVVKVVDGDTVYVVAENGTKYKIRLLGVDTPETHIKNNPYEYYIDKNTPITDVNYLKKWGYKATEFAKNKLENKTVIVVFDNKAPKKDKYGRYLAYIFVSNTTYGIRKPEVFVNFNEELLKYGYARVYISNFELKDEFIKIEKEAKMRRIGLWNWSNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2020-06-17" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEX1</accession>
    <name>F6BEX1_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0147</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95707.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF95707">
        <property type="protein sequence ID" value="AEF95707"/>
        <property type="gene ID" value="Metig_0147"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0147"/>
    <dbReference type="HOGENOM" id="CLU_1801701_0_0_2"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="45"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF95707.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="143" mass="17400" checksum="693AEAF99D385CB4" modified="2011-07-27" version="1">MNPLQTKTKHQVITIEDERIENIYNAIKYLEGINYNISKMHFKDVDRFLDEFNQIKSKYEKMKKDIEHYQKILDRQESIPDNYYEIEEKLKTYKKELQEKIGTDFEEIIKFLKGELNDFNVNEKTLKNFVIYLKPLIKEVLNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2020-08-12" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEZ1</accession>
    <name>F6BEZ1_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_0170</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95727.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF95727">
        <property type="protein sequence ID" value="AEF95727"/>
        <property type="gene ID" value="Metig_0170"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0170"/>
    <dbReference type="HOGENOM" id="CLU_2820824_0_0_2"/>
    <dbReference type="OMA" id="VIEVPYG"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF95727.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="63" mass="7107" checksum="E08F5E13C13657CF" modified="2011-07-27" version="1">MKRFLCSKCKNVIEVPYGVPKPDICPYCNADGIFIHRIDTGGRGLGRGRGRRCGLRFLEKLKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>F6BF18</accession>
    <name>F6BF18_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Pyruvate/ketoisovalerate oxidoreductase, gamma subunit</fullName>
            <ecNumber evidence="3">1.2.7.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_1353</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.2.7.1" evidence="3"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96888.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799485.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1353"/>
    <dbReference type="EnsemblBacteria" id="AEF96888">
        <property type="protein sequence ID" value="AEF96888"/>
        <property type="gene ID" value="Metig_1353"/>
    </dbReference>
    <dbReference type="GeneID" id="10644216"/>
    <dbReference type="KEGG" id="mig:Metig_1353"/>
    <dbReference type="HOGENOM" id="CLU_087284_2_0_2"/>
    <dbReference type="OMA" id="PDYVIVQ"/>
    <dbReference type="OrthoDB" id="95610at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019164">
        <property type="term" value="F:pyruvate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.920.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011894">
        <property type="entry name" value="PorC_KorC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019752">
        <property type="entry name" value="Pyrv/ketoisovalerate_OxRed_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002869">
        <property type="entry name" value="Pyrv_flavodox_OxRed_cen"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01558">
        <property type="entry name" value="POR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53323">
        <property type="entry name" value="SSF53323"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02175">
        <property type="entry name" value="PorC_KorC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="POR" evidence="2">
        <location>
            <begin position="10"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF96888.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="179" mass="19512" checksum="EBB3624D1A284D1F" modified="2011-07-27" version="1">MIEIRFHGRGGQGAVTAAQILAKAAFYDGKYCQAFPFFGVERRGAPVMAFTRIDDKKIRLRCQIYEPDYVVVQDATLLDSVDVTSGLKNDGKVIINTVKDIKLGDYDTYCIDATGIALDVLGVPIVNTTMLGAFAGVTGEVSIESLKKAIMETFPAKLGEKNAKAAEVAYEAMIEKYKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-06-02" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>F6BF38</accession>
    <name>F6BF38_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_1373</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96908.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799504.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96908">
        <property type="protein sequence ID" value="AEF96908"/>
        <property type="gene ID" value="Metig_1373"/>
    </dbReference>
    <dbReference type="GeneID" id="10644238"/>
    <dbReference type="KEGG" id="mig:Metig_1373"/>
    <dbReference type="HOGENOM" id="CLU_2447689_0_0_2"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF96908.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="89" mass="10027" checksum="7C5CD3F98BE68D5F" modified="2011-07-27" version="1">MKKYKNNELLWIFAIPAIILTPNVGLGLKVDDPMEHFVGTPAHKPCDEIGEAKGANVENKNNYFLILKLNLTEVTNIANKNRRFGYAKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>F6BF45</accession>
    <name>F6BF45_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">CO dehydrogenase/acetyl-CoA synthase complex, epsilon subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_1380</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96915.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1380"/>
    <dbReference type="EnsemblBacteria" id="AEF96915">
        <property type="protein sequence ID" value="AEF96915"/>
        <property type="gene ID" value="Metig_1380"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_1380"/>
    <dbReference type="HOGENOM" id="CLU_1773184_0_0_2"/>
    <dbReference type="OMA" id="ITYYYLA"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019385">
        <property type="term" value="P:methanogenesis, from acetate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003704">
        <property type="entry name" value="CO_DH_CoA_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02552">
        <property type="entry name" value="CO_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="SSF52467"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00315">
        <property type="entry name" value="cdhB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF96915.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="147" mass="16822" checksum="F81387E343B0C7AE" modified="2011-07-27" version="1">MVKRMIDRTTPYQPTAGTNLNHADVVSPTILIQMIKRSKNPILIVGCKLDKDVEEILSKLNMKKIHTPKEMNLLDIMKYLAKGEHDLALFIGITYYYLAQALTHLKHFSSVITVTIDPYYHPNACYSLPNMEKNEHVEVIKKLVDAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-09-29" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>F6BF53</accession>
    <name>F6BF53_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="3">DUF234 DEXX-box ATPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_1388</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the archaeal ATPase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96923.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96923">
        <property type="protein sequence ID" value="AEF96923"/>
        <property type="gene ID" value="Metig_1388"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_1388"/>
    <dbReference type="HOGENOM" id="CLU_041137_3_0_2"/>
    <dbReference type="OMA" id="PLKEEPY"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011579">
        <property type="entry name" value="ATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004256">
        <property type="entry name" value="DUF234_ATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01637">
        <property type="entry name" value="ATPase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03008">
        <property type="entry name" value="DUF234"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA" evidence="2">
        <location>
            <begin position="29"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF96923.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="463" mass="54723" checksum="3ED1D1D4A8280A51" modified="2011-07-27" version="1">MIGIILVILMFINRKEELSFLEKLYKSNKKEVLILYGRRRVGKTELIKQFIKNINSENVIYFLADKSGLKTNAERFYEKAAKKFNLPKVKVDDFRDAFNLLKIIPNNRIVVVIDEFSYLLEDKNTSAIFQHIIDEILDDRFFIILCGSLVGLMESLMSYKNPLYGRRTAQLKLSPLNFLHVREYFKNTDIETVVKIYSVTGGIPMYFKLFKGEDFEKELNEVVFSKTSILYEEVEFILREEVGDVHRYYLILEALAKGYNRVSEISSVTGVEAKDLPKYLRVLMSLDLVEREIPITEGLKSKKGRYKIKDNFFRFWFRFVYPNKSEIEIGTYEMDYLSFNKYVGEVFEYIAKEFLIELNKNDNLPFKFSKIGRWWHKGEEVDLITLNENERKALFVEVKWKELSVKEVYGIFKDLERKSELVGLKEYDKYYCVFCKGIKGKIELDVNYLVFDLESLEQYSVSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>F6BF79</accession>
    <name>F6BF79_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="7">ABC-type transporter, integral membrane subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Metig_1414</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96949.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799545.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1414"/>
    <dbReference type="EnsemblBacteria" id="AEF96949">
        <property type="protein sequence ID" value="AEF96949"/>
        <property type="gene ID" value="Metig_1414"/>
    </dbReference>
    <dbReference type="GeneID" id="10644287"/>
    <dbReference type="KEGG" id="mig:Metig_1414"/>
    <dbReference type="HOGENOM" id="CLU_036879_0_2_2"/>
    <dbReference type="OMA" id="GLQLGYM"/>
    <dbReference type="OrthoDB" id="61149at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045621">
        <property type="entry name" value="BPD_transp_1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19300">
        <property type="entry name" value="BPD_transp_1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="9"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="104"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="140"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="174"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="232"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="278"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="6">
        <location>
            <begin position="100"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEF96949.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="311" mass="34293" checksum="B9DC85BF490E9863" modified="2011-07-27" version="1">MKGFIIKRLFLIIPTAIAVSLISFSLLYFSPGNAAEIILEQKSPDAGPNSELVKEYEKKLGLDKPFYELYLIWLSKVLRGDFGTSLQNGEPVIDEIMARLPYTLMLMLGATVVYLILGILMGVLSAVYKDSFIDNIVRAYASINMSIPNFWLALLLIWIFSVKLRIIDTFGYNGVYSLILPSLALGLGMAGSLARVLRVSILEVFNSDYVLTARAKGLSERIIILKHVLKNAFLPVITLIGMKTTYLLGGSVIVESIFGWPGIGSYLIEAINAKDFPAVSGTVFIIGILVVIANLIVDISYTILDPRIRYG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>F6BF80</accession>
    <name>F6BF80_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="6">ABC-type transporter, integral membrane subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Metig_1415</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96950.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799546.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1415"/>
    <dbReference type="EnsemblBacteria" id="AEF96950">
        <property type="protein sequence ID" value="AEF96950"/>
        <property type="gene ID" value="Metig_1415"/>
    </dbReference>
    <dbReference type="GeneID" id="10644288"/>
    <dbReference type="KEGG" id="mig:Metig_1415"/>
    <dbReference type="HOGENOM" id="CLU_028518_5_3_2"/>
    <dbReference type="OMA" id="VHMRESA"/>
    <dbReference type="OrthoDB" id="63271at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="5"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="66"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="103"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="129"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="185"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="230"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="5">
        <location>
            <begin position="64"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEF96950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="268" mass="29557" checksum="5A8C6A9D4FED9CD5" modified="2011-07-27" version="1">MNKRFILSLSMVVLIFLVSILSPYIFKDVNKIDFTEKLQKPSLEHPFGTDNFGRDLFKRTLCGLRISLILAVCIEAISLIIGISLGLIAGYYGGIVDEIISRIIDIVIAFPNIIFALAIVSIFGQSMVVLVLALSLLGWASYARIIRGEVLSLKERDFVTLARTSGGSNFYILIKHILPNAIIPIIPLATLMIGHSVLSIAGLSFLGFGVQPPTPELGLMLKESVTYIDTAPWMMIFPGLVLSFCVLMFNTIGDALRDILDPRENRIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>F6BF82</accession>
    <name>F6BF82_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Oxidoreductase/nitrogenase component 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1417</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96952.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799548.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1417"/>
    <dbReference type="EnsemblBacteria" id="AEF96952">
        <property type="protein sequence ID" value="AEF96952"/>
        <property type="gene ID" value="Metig_1417"/>
    </dbReference>
    <dbReference type="GeneID" id="10644290"/>
    <dbReference type="KEGG" id="mig:Metig_1417"/>
    <dbReference type="HOGENOM" id="CLU_025876_4_1_2"/>
    <dbReference type="OMA" id="RMLHRWR"/>
    <dbReference type="OrthoDB" id="10827at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000510">
        <property type="entry name" value="Nase/OxRdtase_comp1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00148">
        <property type="entry name" value="Oxidored_nitro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Oxidored_nitro" evidence="1">
        <location>
            <begin position="7"/>
            <end position="401"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96952.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="404" mass="45503" checksum="FA48BDAB4AFE554A" modified="2011-07-27" version="1">MEPLQGCRMIGAVRAFNGILDGYIILHSPPGCHSGVMMLEVLQDNSDWRVAVSGMHQRDLVYGAENKCLLAIKKVYETIKPNFIVVMDCCSSGILGEDLESVVNMAKREIGEDMDIIYFSGAGYHSLAYLGYEEALKSLVQFMEQKETIPNSINLIGIKIDDFKAKEDIEEIKRICSNAGVNINSILTCDTFDSIKNAPNAELNVVFGDGIKLAELMKKKFDIPYVVVDYPYGLNGTLEFLNSLNEYVNVDFKFVEEEKKRIIKTVEKVQFYLKGFYGMNSAVVGDYKAFGFAKFLSDEIGFDIDTLGISKFYNIDSKIKEIKHIVENVVIDDRKELEDKIMENDIEILFGSSYEKKIAQNKNIPLIRFSYPVVDEISLSNTPLAGFRGVSVIIERIINEIIKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-10-19" modified="2022-01-19" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>G1UBJ2</accession>
    <name>G1UBJ2_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">50S ribosomal protein L1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">rpl1</name>
        <name evidence="8" type="ordered locus">Metig_0941</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Binds directly to 23S rRNA. Probably involved in E site tRNA release.</text>
    </comment>
    <comment type="function">
        <text evidence="5">Probably involved in E site tRNA release (By similarity). Binds directly to 23S rRNA.</text>
    </comment>
    <comment type="function">
        <text evidence="6">Protein L1 is also a translational repressor protein, it controls the translation of its operon by binding to its mRNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 6">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6 7">Belongs to the universal ribosomal protein uL1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96484.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799086.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0941"/>
    <dbReference type="EnsemblBacteria" id="AEF96484">
        <property type="protein sequence ID" value="AEF96484"/>
        <property type="gene ID" value="Metig_0941"/>
    </dbReference>
    <dbReference type="GeneID" id="10643787"/>
    <dbReference type="KEGG" id="mig:Metig_0941"/>
    <dbReference type="HOGENOM" id="CLU_062853_4_0_2"/>
    <dbReference type="OMA" id="GWTDVDV"/>
    <dbReference type="OrthoDB" id="70269at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015934">
        <property type="term" value="C:large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00403">
        <property type="entry name" value="Ribosomal_L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01318_A">
        <property type="entry name" value="Ribosomal_L1_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002143">
        <property type="entry name" value="Ribosomal_L1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023674">
        <property type="entry name" value="Ribosomal_L1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028364">
        <property type="entry name" value="Ribosomal_L1/biogenesis"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016095">
        <property type="entry name" value="Ribosomal_L1_3-a/b-sand"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023669">
        <property type="entry name" value="Ribosomal_L1_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023673">
        <property type="entry name" value="Ribosomal_L1_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00687">
        <property type="entry name" value="Ribosomal_L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002155">
        <property type="entry name" value="Ribosomal_L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56808">
        <property type="entry name" value="SSF56808"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01199">
        <property type="entry name" value="RIBOSOMAL_L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0678">Repressor</keyword>
    <keyword evidence="4 6 7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 6 7" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6" id="KW-0699">rRNA-binding</keyword>
    <keyword evidence="6" id="KW-0810">Translation regulation</keyword>
    <keyword evidence="6" id="KW-0820">tRNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00024742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01318"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEF96484.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="213" mass="23946" checksum="2785E095C45AE94F" modified="2011-10-19" version="1">MDREKILQAVKEARRLAKPRNFTQSFDLVVNLKELDLSRPENRLKEEVVLPHGRGKEAKIAVIAKGDLAAQAEELGLTVIREEELEELGKDKRKARKVANAHDFFIAQADMMPLVGRYLGPILGPRGKMPKPVPANINLKPLVERLKKTVVVNTRDKPIFHVLVGNEKMSDEQVAENIEAILNTVAKKYEKGLYHVKSAYVKLTMGPAVKIEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>F6BA64</accession>
    <name>F6BA64_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">CCA-adding enzyme</fullName>
            <ecNumber evidence="8">2.7.7.72</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">CCA tRNA nucleotidyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">tRNA CCA-pyrophosphorylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">tRNA adenylyl-/cytidylyl- transferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">tRNA nucleotidyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">tRNA-NT</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">cca</name>
        <name evidence="11" type="ordered locus">Metig_0197</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the addition and repair of the essential 3'-terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing inorganic pyrophosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>a tRNA precursor + ATP + 2 CTP = a tRNA with a 3' CCA end + 3 diphosphate</text>
            <dbReference type="Rhea" id="RHEA:14433"/>
            <dbReference type="Rhea" id="RHEA-COMP:10465"/>
            <dbReference type="Rhea" id="RHEA-COMP:10468"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:74896"/>
            <dbReference type="ChEBI" id="CHEBI:83071"/>
            <dbReference type="EC" id="2.7.7.72"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 8">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="8">A single active site specifically recognizes both ATP and CTP and is responsible for their addition.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Archaeal CCA-adding enzyme subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.72" evidence="8"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95754.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798363.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0197"/>
    <dbReference type="EnsemblBacteria" id="AEF95754">
        <property type="protein sequence ID" value="AEF95754"/>
        <property type="gene ID" value="Metig_0197"/>
    </dbReference>
    <dbReference type="GeneID" id="10643032"/>
    <dbReference type="KEGG" id="mig:Metig_0197"/>
    <dbReference type="HOGENOM" id="CLU_044679_1_0_2"/>
    <dbReference type="OMA" id="DVDLVPC"/>
    <dbReference type="OrthoDB" id="82755at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052929">
        <property type="term" value="F:ATP:3'-cytidine-cytidine-tRNA adenylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052928">
        <property type="term" value="F:CTP:3'-cytidine-tRNA cytidylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052927">
        <property type="term" value="F:CTP:tRNA cytidylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016437">
        <property type="term" value="F:tRNA cytidylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042245">
        <property type="term" value="P:RNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001680">
        <property type="term" value="P:tRNA 3'-terminal CCA addition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd05400">
        <property type="entry name" value="NT_2-5OAS_ClassI-CCAase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1410.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01264">
        <property type="entry name" value="CCA_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008229">
        <property type="entry name" value="CCA-adding_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042090">
        <property type="entry name" value="CCA_tRNA_nucleotrans_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006116">
        <property type="entry name" value="NT_2-5OAS_ClassI-CCAase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011068">
        <property type="entry name" value="NuclTrfase_I-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002934">
        <property type="entry name" value="Polymerase_NTP_transf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015329">
        <property type="entry name" value="tRNA_NucTransf2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39643">
        <property type="entry name" value="PTHR39643"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01909">
        <property type="entry name" value="NTP_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09249">
        <property type="entry name" value="tRNA_NucTransf2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005335">
        <property type="entry name" value="CCA_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55003">
        <property type="entry name" value="SSF55003"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="SSF81301"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03671">
        <property type="entry name" value="cca_archaeal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8 11" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 8" id="KW-0692">RNA repair</keyword>
    <keyword evidence="8" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2 8 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 8" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="NTP_transf_2" evidence="9">
        <location>
            <begin position="32"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="domain" description="tRNA_NucTransf2" evidence="10">
        <location>
            <begin position="162"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="56"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="59"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="68"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="70"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="123"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="146"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="168"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF09249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AEF95754.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="448" mass="53157" checksum="34123A8729FE4481" modified="2011-07-27" version="1">MNIDEILEEVLDDIKPTEEEQRELKEMSSKIINKINEIIKQNPKYSVIIDVVQVGSTARNTHLRNNYDIDIFLVFDKNTEREVLEEIGLEVGKKVVEELGGEYWTEYAEHPYTSAVIDKYSLDIVPCYRIDWGEKIISAVDRTPLHNEFVKRKLEKNERLCDEVRLFKKFLKSIGIYGSDLKTKGFSGYLCELLVIHYGGFIEVLKNAKDWKIGEKIILEDIYKIYNLDKNYKFRKFDAPLIVYDPVDINRNVAAALSKDNFCKFVFYASLFLKNPSKGFFYDYYKHVDQKLEDREHGALLTLKIKRDKDIVDDIIYPQMEKLQRSINKIIEENEFVILRCDVHADEEFCYLSWEFLVYELPNVRLREGPSIFKKERVERFIRKYDKYFIRDCKLYAYTTRKYKNVYELFNDIIHGKLRGKISYPKYVNPENGVICKMYVKDTIKVAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-06-02" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>F6BA92</accession>
    <name>F6BA92_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_0225</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95782.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798391.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF95782">
        <property type="protein sequence ID" value="AEF95782"/>
        <property type="gene ID" value="Metig_0225"/>
    </dbReference>
    <dbReference type="GeneID" id="10643060"/>
    <dbReference type="KEGG" id="mig:Metig_0225"/>
    <dbReference type="HOGENOM" id="CLU_887427_0_0_2"/>
    <dbReference type="OMA" id="YFGKWNI"/>
    <dbReference type="OrthoDB" id="46740at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF95782.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="316" mass="37808" checksum="AC50B2016B2D42A1" modified="2011-07-27" version="1">MTLLDICNEIIEGEDGKVKDFAHTIKLTYLSEFERFEKEDMKVKLRKLNIAEEDGLLFYGKDYLIFKSIYYFNEVPVFRKEEDAIIFLNKIGIEPNRTLKSLSFEEKRKLGNEFLNKALICVPKEYSKYLPYIIFGKEYYFKGIELKEYVSSLNGLYKIGKRKKVRDLIVNMEIPDEDDVKKYKKKIAKRINKFKKKLNDEYEINYFNLKFKGKKFKCQYIYIKPSLWDHVKSFFGEGIELKYYPTLINVAYSSEKIDFLKPLFIFVDKKDVAVYAKVPKLVYLKNNLSLNHLNLEGKYIFYGNWSDEEFYKFLKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-09-29" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>F6BA98</accession>
    <name>F6BA98_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="4">AAA ATPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metig_0231</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the archaeal ATPase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95788.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798397.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF95788">
        <property type="protein sequence ID" value="AEF95788"/>
        <property type="gene ID" value="Metig_0231"/>
    </dbReference>
    <dbReference type="GeneID" id="10643066"/>
    <dbReference type="KEGG" id="mig:Metig_0231"/>
    <dbReference type="HOGENOM" id="CLU_068608_0_0_2"/>
    <dbReference type="OMA" id="VINEMKY"/>
    <dbReference type="OrthoDB" id="50712at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011579">
        <property type="entry name" value="ATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01637">
        <property type="entry name" value="ATPase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA" evidence="3">
        <location>
            <begin position="21"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="267"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEF95788.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="348" mass="41266" checksum="3140D13F1B877567" modified="2011-07-27" version="1">MKFFNRDKEIKKILSIIESEPNFIYFLYGPINSGKTTLINEIINRLDKDRYVVFYINLRRYFVSKYRDFIEVLFEEYEEDKNPIELIKSLIKDSSIIYGIPVPKNTLEVLTSKKDSKNIFRYITDILMKIKESGKQPIIVIDELQKIGDLEINGYLIYELFNYFISLTKEMHLSHVFCLSSDSLFIERVYSEAMLEDRADYILVDDFDKETALKFMDFLAGEVLNRALSEEKKELIYNYVGGKPVLIIKVINKMRAEELDEILNFMLNDTKQRLKYSLEDIREENEELYKEIIKALSLFKESYEVEDITIDKKVREFLVKRNILFLDTIKGVIKPQSFLIWNAIKILI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-06-02" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAA1</accession>
    <name>F6BAA1_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_0234</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95791.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF95791">
        <property type="protein sequence ID" value="AEF95791"/>
        <property type="gene ID" value="Metig_0234"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0234"/>
    <dbReference type="HOGENOM" id="CLU_2712840_0_0_2"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF95791.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="72" mass="8504" checksum="7D7AFBBB012E27D3" modified="2011-07-27" version="1">MSFRYIYIGLYKDKKNRWVELGVISERSDENLKTVRFLCPRQGQRHSRVADDAQVQYGYLLTNTHIGQTPNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAA5</accession>
    <name>F6BAA5_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glycosyl transferase group 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0238</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95795.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF95795">
        <property type="protein sequence ID" value="AEF95795"/>
        <property type="gene ID" value="Metig_0238"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0238"/>
    <dbReference type="HOGENOM" id="CLU_736949_0_0_2"/>
    <dbReference type="OMA" id="DICENGW"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glycos_transf_1" evidence="1">
        <location>
            <begin position="206"/>
            <end position="366"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF95795.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="385" mass="46151" checksum="380FF190096ACC6E" modified="2011-07-27" version="1">MEDFINKGVKMKKILVLYQDWDNWFLNSYEKFEHWFKEKDGAYSKDNEYYILSLSTCNKILKPENNITVELFKSSPTKQLFDLIKFKKRLKEVINEFKPDYIYVPFLYLASTIPKDRNFKVISFLRDITPEMIKGKGGIRKILGNIFYILDYLALKKTDILLYNAFHLKEYALKMRYKGKLIFCPRDITDKEYFNEISEKEIIEIVRKYNLKNKRIILTVARLTPEKNIEMGIKALRFLPKDYVYIIVGEGRDEKRLVNLAKKLGVYDRVVFIGYVKHKEIWKYYKVADVLWLLSKSNFEGIPNVIMESWYSKTPVIVSNIKVFKYLIKNYKTGIVLKSWDEKELAEKTEELLKNNKLYKDICENGWKYVNELIRHHIDVRELFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAA6</accession>
    <name>F6BAA6_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Methyltransferase type 12</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0239</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95796.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF95796">
        <property type="protein sequence ID" value="AEF95796"/>
        <property type="gene ID" value="Metig_0239"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0239"/>
    <dbReference type="HOGENOM" id="CLU_106195_0_0_2"/>
    <dbReference type="OMA" id="DYPYHEE"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025714">
        <property type="entry name" value="Methyltranfer_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13847">
        <property type="entry name" value="Methyltransf_31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltranfer_dom" evidence="1">
        <location>
            <begin position="4"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF95796.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="168" mass="19789" checksum="0F406720B06F7D0A" modified="2011-07-27" version="1">MWGLDIDKERISQLKNKGYNVIYDDVQKFENLKKLNKKFDVIVAGEIIEHLENPGLFLDKVKEFLKEDGILIITTPNMLSLRFIIRHTLFGQESPFWKNRDDEIRYGHVIGFSKMLLENLLLRKGYKILEIRYTIKDEYSGVKGNIEKLISKIFPRFSPNLIVICKIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAD6</accession>
    <name>F6BAD6_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Pantoate kinase</fullName>
            <shortName evidence="1">PoK</shortName>
            <ecNumber evidence="1">2.7.1.169</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0270</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Phosphorylates (R)-pantoate to form (R)-4-phosphopantoate in the CoA biosynthesis pathway.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(R)-pantoate + ATP = (R)-4-phosphopantoate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:28246"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15980"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61294"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.169"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; coenzyme A biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the GHMP kinase family. PoK subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.169" evidence="1"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95826.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798435.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0270"/>
    <dbReference type="EnsemblBacteria" id="AEF95826">
        <property type="protein sequence ID" value="AEF95826"/>
        <property type="gene ID" value="Metig_0270"/>
    </dbReference>
    <dbReference type="GeneID" id="10643105"/>
    <dbReference type="KEGG" id="mig:Metig_0270"/>
    <dbReference type="HOGENOM" id="CLU_081191_0_0_2"/>
    <dbReference type="OMA" id="MLGETVF"/>
    <dbReference type="OrthoDB" id="104702at2157"/>
    <dbReference type="UniPathway" id="UPA00241"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015937">
        <property type="term" value="P:coenzyme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02223">
        <property type="entry name" value="Pantoate_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012043">
        <property type="entry name" value="PoK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42282">
        <property type="entry name" value="PTHR42282"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016896">
        <property type="entry name" value="GHMP_arc_MJ0969"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0173">Coenzyme A biosynthesis</keyword>
    <keyword evidence="1 2" id="KW-0418">Kinase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF95826.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="278" mass="30984" checksum="1F0F12B9C272F53C" modified="2011-07-27" version="1">MFVPAHITGFFKIFKDRDFLKTGSTGAGITLDKGVHTTIKPGNGDIYFNNKKIDLSPTREVMDYFNFGGSYDVMHFSDFPLGSGLGTSGGCALGTAHEVFKIIDTYKIYDDNNYNNEAVKIAHIAEVKCGTGLGDVIAQHTKGFVIRKKPGFPINVEKVKINNIDDYYVVVEILGKKETKNIINNPQWIEKINKTSDELLIKLLKNPTLKNFMDLSLYFAKTTGLISDNILEICKDLKFTIGASQAMLGNTVFCICKKEDLDDVLSILKNPIVCKIYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAE9</accession>
    <name>F6BAE9_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Lysine--tRNA ligase</fullName>
            <ecNumber evidence="9">6.1.1.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Lysyl-tRNA synthetase</fullName>
            <shortName evidence="9">LysRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">lysS</name>
        <name evidence="11" type="ordered locus">Metig_1427</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>ATP + L-lysine + tRNA(Lys) = AMP + diphosphate + L-lysyl-tRNA(Lys)</text>
            <dbReference type="Rhea" id="RHEA:20792"/>
            <dbReference type="Rhea" id="RHEA-COMP:9696"/>
            <dbReference type="Rhea" id="RHEA-COMP:9697"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:32551"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78529"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.6"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.6" evidence="9"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96962.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799558.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1427"/>
    <dbReference type="EnsemblBacteria" id="AEF96962">
        <property type="protein sequence ID" value="AEF96962"/>
        <property type="gene ID" value="Metig_1427"/>
    </dbReference>
    <dbReference type="GeneID" id="10644300"/>
    <dbReference type="KEGG" id="mig:Metig_1427"/>
    <dbReference type="HOGENOM" id="CLU_025562_1_0_2"/>
    <dbReference type="OMA" id="DWPMRWA"/>
    <dbReference type="OrthoDB" id="9880at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004824">
        <property type="term" value="F:lysine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006430">
        <property type="term" value="P:lysyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.350">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00177">
        <property type="entry name" value="Lys_tRNA_synth_class1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045462">
        <property type="entry name" value="aa-tRNA-synth_I_cd-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020751">
        <property type="entry name" value="aa-tRNA-synth_I_codon-bd_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008925">
        <property type="entry name" value="aa_tRNA-synth_I_cd-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002904">
        <property type="entry name" value="Lys-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042078">
        <property type="entry name" value="Lys-tRNA-ligase_SC_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37940">
        <property type="entry name" value="PTHR37940"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19269">
        <property type="entry name" value="Anticodon_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01921">
        <property type="entry name" value="tRNA-synt_1f"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48163">
        <property type="entry name" value="SSF48163"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00467">
        <property type="entry name" value="lysS_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9 11" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 9 11" id="KW-0436">Ligase</keyword>
    <keyword evidence="5 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 9" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Anticodon_2" evidence="10">
        <location>
            <begin position="440"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'HIGH' region" evidence="9">
        <location>
            <begin position="28"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'KMSKS' region" evidence="9">
        <location>
            <begin position="282"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF19269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AEF96962.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="532" mass="61968" checksum="819A9932C98EA96D" modified="2011-07-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAF1</accession>
    <name>F6BAF1_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">2-oxoglutarate synthase</fullName>
            <ecNumber evidence="2">1.2.7.3</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1429</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.2.7.3" evidence="2"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96964.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799560.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1429"/>
    <dbReference type="EnsemblBacteria" id="AEF96964">
        <property type="protein sequence ID" value="AEF96964"/>
        <property type="gene ID" value="Metig_1429"/>
    </dbReference>
    <dbReference type="GeneID" id="10644302"/>
    <dbReference type="KEGG" id="mig:Metig_1429"/>
    <dbReference type="HOGENOM" id="CLU_048564_2_0_2"/>
    <dbReference type="OMA" id="SAWIASP"/>
    <dbReference type="OrthoDB" id="43666at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047553">
        <property type="term" value="F:2-oxoglutarate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006082">
        <property type="term" value="P:organic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901576">
        <property type="term" value="P:organic substance biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044272">
        <property type="term" value="P:sulfur compound biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011766">
        <property type="entry name" value="TPP_enzyme-bd_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02775">
        <property type="entry name" value="TPP_enzyme_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="SSF52518"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TPP_enzyme_C" evidence="1">
        <location>
            <begin position="50"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96964.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="266" mass="29457" checksum="F226A07CB8056DBA" modified="2011-07-27" version="1">MHPSLKYMRTDRLPHIFCSGCGNGIVINCFLNAIEKLNIKPEDYIAVSGIGCSSRIPGYLYCDSLHTTHGRPIAFALGIKVARPDKKIVVFTGDGDLAAIGGNHFIHGCRRNIDMTVICINNNIYGMTGGQCSPTTPHKKKATTAPYGNPENPMDLCKLAEAAGATYVARWTTAHPIQLVNAIKKALQKKGFSFIEVVSQCPTYYGRFNVSRKPAEMMKFLKESSITINRAKDMSEEELNGKIIVGEFVDIEKPEFIEELKKLQQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAG0</accession>
    <name>F6BAG0_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 7">Probable cobyric acid synthase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">cobQ</name>
        <name evidence="10" type="ordered locus">Metig_1438</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6 7">Catalyzes amidations at positions B, D, E, and G on adenosylcobyrinic A,C-diamide. NH(2) groups are provided by glutamine, and one molecule of ATP is hydrogenolyzed for each amidation.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 7">Cofactor biosynthesis; adenosylcobalamin biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the CobB/CobQ family. CobQ subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96973.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799569.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1438"/>
    <dbReference type="EnsemblBacteria" id="AEF96973">
        <property type="protein sequence ID" value="AEF96973"/>
        <property type="gene ID" value="Metig_1438"/>
    </dbReference>
    <dbReference type="GeneID" id="10644311"/>
    <dbReference type="KEGG" id="mig:Metig_1438"/>
    <dbReference type="HOGENOM" id="CLU_019250_2_2_2"/>
    <dbReference type="OMA" id="EIHHGVA"/>
    <dbReference type="OrthoDB" id="34382at2157"/>
    <dbReference type="UniPathway" id="UPA00148"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015420">
        <property type="term" value="F:ABC-type vitamin B12 transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006541">
        <property type="term" value="P:glutamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01750">
        <property type="entry name" value="GATase1_CobQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00028">
        <property type="entry name" value="CobQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002586">
        <property type="entry name" value="CobQ/CobB/MinD/ParA_Nub-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033949">
        <property type="entry name" value="CobQ_GATase1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004459">
        <property type="entry name" value="CobQ_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011698">
        <property type="entry name" value="GATase_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21343:SF1">
        <property type="entry name" value="PTHR21343:SF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01656">
        <property type="entry name" value="CbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07685">
        <property type="entry name" value="GATase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="SSF52317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00313">
        <property type="entry name" value="cobQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword evidence="5 7" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CbiA" evidence="8">
        <location>
            <begin position="5"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="domain" description="GATase_3" evidence="9">
        <location>
            <begin position="255"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="7">
        <location>
            <position position="330"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="436"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00014921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF07685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AEF96973.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="492" mass="55164" checksum="595C7FEDFD2462D6" modified="2011-07-27" version="1">MAKFIMVVGTSSNSGKTVLTSALCRILANRGYKVAPFKSQNMSLNSRVAKEDGEIAVAQYTQAKAAKTEPSIHFNPILLKPKGNFISQVIVHGKPYKDMDYNEYRKNKDYFLKKIKESLDYLDKNYDYVVMEGAGSCCEINLLDDDIANLRIAEMVNAKAILVADIDRGGVFASIYGTIELLPKNWRKLIKGIVINKFRGNVDVLKSGIEKIEELTGIPVLGVVPYDEHLILPEEDSQALQSKKTFGNLNSKVEVNVLRFSKISNFTDVDALSRDAFIKFIDFGEDITGDILILPGTRTSTKEMYLIKKYGMDKKIWEFIRRGGIVLGICGGYQVMGKMLVDENKKESEVGTIEGLGIFDAVTYFGNEKAIKNSKGVLEIDGKVFEVEGYELHEGITHSNEKPLIKLEKGFGNRGDGFDGSIKKIGDAYLIGTYFHGILDNYEFRNYLINIVRKRKGLKPIKGDNYRKVFEENMDKLAKIVENSVNLDSIIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-09-29" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAG6</accession>
    <name>F6BAG6_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Metig_1444</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="5">Belongs to the HepT RNase toxin family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96979.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799575.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96979">
        <property type="protein sequence ID" value="AEF96979"/>
        <property type="gene ID" value="Metig_1444"/>
    </dbReference>
    <dbReference type="GeneID" id="10644317"/>
    <dbReference type="KEGG" id="mig:Metig_1444"/>
    <dbReference type="HOGENOM" id="CLU_152343_0_0_2"/>
    <dbReference type="OMA" id="MDIVAML"/>
    <dbReference type="OrthoDB" id="113565at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110001">
        <property type="term" value="C:toxin-antitoxin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004540">
        <property type="term" value="F:ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.580">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008201">
        <property type="entry name" value="HepT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037038">
        <property type="entry name" value="HepT-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01934">
        <property type="entry name" value="DUF86"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-1277">Toxin-antitoxin system</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00024207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEF96979.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="133" mass="15943" checksum="E46327B9868AB771" modified="2011-07-27" version="1">MERKKRYLEKLEKFEEEYYFIKEHKIEDEVTKRALLYSLQVCVDVAMDVVAMLVKDVGLNVEDDYTNIEKLLEHNIIAKEEATLLKQYNGLRNAIVHKYDKLNLEVVKEGLKRIDELYEIIIKLIEFYEKVEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAH1</accession>
    <name>F6BAH1_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative signal transduction protein with CBS domains</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_1449</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96984.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799580.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1449"/>
    <dbReference type="EnsemblBacteria" id="AEF96984">
        <property type="protein sequence ID" value="AEF96984"/>
        <property type="gene ID" value="Metig_1449"/>
    </dbReference>
    <dbReference type="GeneID" id="10644322"/>
    <dbReference type="KEGG" id="mig:Metig_1449"/>
    <dbReference type="HOGENOM" id="CLU_040681_12_0_2"/>
    <dbReference type="OMA" id="WDKQVHQ"/>
    <dbReference type="OrthoDB" id="104239at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="SSF54631"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0129">CBS domain</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBS" evidence="2">
        <location>
            <begin position="7"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="2">
        <location>
            <begin position="103"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF96984.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="153" mass="17031" checksum="6D79A7B2A9FFDAEF" modified="2011-07-27" version="1">MIKVRDVMKKPITVNPDDDIRDVIRLFREHKISGAPVVEDGKLVGIISESDIVKTITTHNESIGLILPSPLDLIELPLKTTLKIEEFKEDIKKALKTKVKDVMTKDVITISPDETINDAAKLMIENNIKRLPVVENGKLVGIVTRGDLIEALC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAI4</accession>
    <name>F6BAI4_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">CutA1 divalent ion tolerance protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1462</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CutA family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96997.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799593.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1462"/>
    <dbReference type="EnsemblBacteria" id="AEF96997">
        <property type="protein sequence ID" value="AEF96997"/>
        <property type="gene ID" value="Metig_1462"/>
    </dbReference>
    <dbReference type="GeneID" id="10644335"/>
    <dbReference type="KEGG" id="mig:Metig_1462"/>
    <dbReference type="HOGENOM" id="CLU_098807_3_1_2"/>
    <dbReference type="OMA" id="VYTTFPD"/>
    <dbReference type="OrthoDB" id="110178at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010038">
        <property type="term" value="P:response to metal ion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004323">
        <property type="entry name" value="Ion_tolerance_CutA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011322">
        <property type="entry name" value="N-reg_PII-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015867">
        <property type="entry name" value="N-reg_PII/ATP_PRibTrfase_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23419">
        <property type="entry name" value="PTHR23419"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03091">
        <property type="entry name" value="CutA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54913">
        <property type="entry name" value="SSF54913"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96997.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="102" mass="11825" checksum="7363DA2CEC3073A4" modified="2011-07-27" version="1">MYVLVYITASNHEVAKKIASHLLESKLVACANIFPIESMYWWRGKIENDNEVAIILKTKEKLVKKIIEEVKKLHSYTNPCIIAIPIIHGSEEFLKWIDEETI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAL3</accession>
    <name>F6BAL3_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="3">THUMP domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_1492</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97026.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799621.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1492"/>
    <dbReference type="EnsemblBacteria" id="AEF97026">
        <property type="protein sequence ID" value="AEF97026"/>
        <property type="gene ID" value="Metig_1492"/>
    </dbReference>
    <dbReference type="GeneID" id="10644365"/>
    <dbReference type="KEGG" id="mig:Metig_1492"/>
    <dbReference type="HOGENOM" id="CLU_775255_0_0_2"/>
    <dbReference type="OMA" id="NCVVDIG"/>
    <dbReference type="OrthoDB" id="40605at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006400">
        <property type="term" value="P:tRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002877">
        <property type="entry name" value="RNA_MeTrfase_FtsJ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004114">
        <property type="entry name" value="THUMP_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01728">
        <property type="entry name" value="FtsJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02926">
        <property type="entry name" value="THUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00981">
        <property type="entry name" value="THUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51165">
        <property type="entry name" value="THUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="THUMP" evidence="2">
        <location>
            <begin position="51"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF97026.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="344" mass="40283" checksum="3C753640AFE7E1EC" modified="2011-07-27" version="1">MKPTLLVTTQPGFEGYLKEELKTLSIKKIIKYTMFRGLLKVLSESPYETIEAIKKEKDKFKFLMRAVPLEVECPSDLESIKENTIFLLKRKLLKNKLITGKSFVVRCNRRGKHKYTSEEVERFIGKEVLKSFDLKVDLKNYDFKINIEILQDTAYISIFDDNFNEFRHEENIKKSKYLKRYIERPLNRSERKMRELVERYPNIFDVDVVIDIGSAPGGWAKVLAENAKVVYAIDTGELKIKKDNIIHIKKRAEDVEDLPQCQLLTNDTNLYPEESALLTVKLSKFLEENGYIIHTIKSENKYNVKEDIDKVKKIFENANIKILKIMNLRANTKNEVTLMCQKRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAL5</accession>
    <name>F6BAL5_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Uridylate kinase</fullName>
            <shortName evidence="6">UK</shortName>
            <ecNumber evidence="6">2.7.4.22</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Uridine monophosphate kinase</fullName>
            <shortName evidence="6">UMP kinase</shortName>
            <shortName evidence="6">UMPK</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">pyrH</name>
        <name evidence="8" type="ordered locus">Metig_1494</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes the reversible phosphorylation of UMP to UDP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 6">
            <text>ATP + UMP = ADP + UDP</text>
            <dbReference type="Rhea" id="RHEA:24400"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.22"/>
        </reaction>
    </comment>
    <comment type="activity regulation">
        <text evidence="6">Inhibited by UTP.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 6">Pyrimidine metabolism; CTP biosynthesis via de novo pathway; UDP from UMP (UMPK route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homohexamer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 6">Belongs to the UMP kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.22" evidence="6"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97028.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799622.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1494"/>
    <dbReference type="EnsemblBacteria" id="AEF97028">
        <property type="protein sequence ID" value="AEF97028"/>
        <property type="gene ID" value="Metig_1494"/>
    </dbReference>
    <dbReference type="GeneID" id="10644367"/>
    <dbReference type="KEGG" id="mig:Metig_1494"/>
    <dbReference type="HOGENOM" id="CLU_079546_0_0_2"/>
    <dbReference type="OMA" id="MGGTHPG"/>
    <dbReference type="OrthoDB" id="48934at2157"/>
    <dbReference type="UniPathway" id="UPA00159">
        <property type="reaction ID" value="UER00275"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033862">
        <property type="term" value="F:UMP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044210">
        <property type="term" value="P:'de novo' CTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04253">
        <property type="entry name" value="AAK_UMPK-PyrH-Pf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1160.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01220_A">
        <property type="entry name" value="PyrH_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036393">
        <property type="entry name" value="AceGlu_kinase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001048">
        <property type="entry name" value="Asp/Glu/Uridylate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011817">
        <property type="entry name" value="Uridylate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011818">
        <property type="entry name" value="Uridylate_kinase_arch/spir"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00696">
        <property type="entry name" value="AA_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005650">
        <property type="entry name" value="Uridylate_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53633">
        <property type="entry name" value="SSF53633"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02076">
        <property type="entry name" value="pyrH_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 8" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 6" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="AA_kinase" evidence="7">
        <location>
            <begin position="1"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="9"/>
            <end position="10"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="46"/>
        </location>
        <ligand>
            <name>UMP</name>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="47"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="51"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="68"/>
        </location>
        <ligand>
            <name>UMP</name>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="116"/>
            <end position="122"/>
        </location>
        <ligand>
            <name>UMP</name>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="142"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="143"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="148"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="151"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEF97028.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="228" mass="24308" checksum="FD580902BE5BB35C" modified="2011-07-27" version="1">MRAVFALGGSVIMPKDGASVEKIKEYAETFKKIKDLGHDVCIVVGGGWTARNYISVARELKANESFCDEIGIMATRMNSMLLIAALGDYAMKKVPTSFQEAETILNMGKIVVMGGTHPAHTTDAVAASLAEFIDADLLIIATNVDGVYDKDPNKYADAKKLTKITTKELVDLTISASIKAGSSGVVDPLASKIIDRAKMRAVVVKGVPEEILKAVIGGHNGTVIEPEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAM2</accession>
    <name>F6BAM2_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Phosphate transport system permease protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">Metig_0280</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Part of the binding-protein-dependent transport system for phosphate; probably responsible for the translocation of the substrate across the membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cell membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the binding-protein-dependent transport system permease family. CysTW subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95836.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798445.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0280"/>
    <dbReference type="EnsemblBacteria" id="AEF95836">
        <property type="protein sequence ID" value="AEF95836"/>
        <property type="gene ID" value="Metig_0280"/>
    </dbReference>
    <dbReference type="GeneID" id="10643115"/>
    <dbReference type="KEGG" id="mig:Metig_0280"/>
    <dbReference type="HOGENOM" id="CLU_033621_1_3_2"/>
    <dbReference type="OMA" id="VIRMSVL"/>
    <dbReference type="OrthoDB" id="37194at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005315">
        <property type="term" value="F:inorganic phosphate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006817">
        <property type="term" value="P:phosphate ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011864">
        <property type="entry name" value="Phosphate_PstC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02138">
        <property type="entry name" value="phosphate_pstC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0592">Phosphate transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="21"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="82"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="115"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="168"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="225"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="285"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="10">
        <location>
            <begin position="78"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU363054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AEF95836.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="317" mass="35121" checksum="4632862F9059805D" modified="2011-07-27" version="1">MGLKKIMKIIKERNIDFFKIITAPAIIAVFLIIVLMLGVYLTYAWPAIAKYGINLFIDNIWQASEDPSKEVYGLAAPIWGSIYSAIIALLVALPLAICYAIFVNDYAPKKIKYPLIIISDIMAGLPTIIYGIWGAVVLVPILRDYIMKFLYENFSFIPLFNYPPLMGYSYLAAGVLLGIMVTPFAAAIIREAYAMIPSIYKEGLIALGATKYEATKVLIKFIKPAIISSMVLAFGRALGETVAVSLVIGNSFNLTYKLFAPGYTISSLICNQYGNAALYKYMTSVLYAAGLVLFVIGLVVNVIGIYYLKRWRENVSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAN6</accession>
    <name>F6BAN6_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="4">KH-domain/beta-lactamase-domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metig_0294</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95850.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798459.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0294"/>
    <dbReference type="EnsemblBacteria" id="AEF95850">
        <property type="protein sequence ID" value="AEF95850"/>
        <property type="gene ID" value="Metig_0294"/>
    </dbReference>
    <dbReference type="GeneID" id="10643129"/>
    <dbReference type="KEGG" id="mig:Metig_0294"/>
    <dbReference type="HOGENOM" id="CLU_009673_5_1_2"/>
    <dbReference type="OMA" id="YLDGMIW"/>
    <dbReference type="OrthoDB" id="5321at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019975">
        <property type="entry name" value="aCPSF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022712">
        <property type="entry name" value="Beta_Casp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033769">
        <property type="entry name" value="KH_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015946">
        <property type="entry name" value="KH_dom-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004044">
        <property type="entry name" value="KH_dom_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011108">
        <property type="entry name" value="RMMBL"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10996">
        <property type="entry name" value="Beta-Casp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07650">
        <property type="entry name" value="KH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17214">
        <property type="entry name" value="KH_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07521">
        <property type="entry name" value="RMMBL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01027">
        <property type="entry name" value="Beta-Casp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="SSF56281"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03675">
        <property type="entry name" value="arCOG00543"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="KH" evidence="1">
        <location>
            <begin position="95"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="domain" description="Lactamase_B" evidence="2">
        <location>
            <begin position="192"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="domain" description="Beta-Casp" evidence="3">
        <location>
            <begin position="420"/>
            <end position="544"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEF95850.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="635" mass="71569" checksum="428FD048EB7BC96B" modified="2011-07-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAT3</accession>
    <name>F6BAT3_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Phosphoribosylformylglycinamidine synthase subunit PurS</fullName>
            <shortName evidence="5">FGAM synthase</shortName>
            <ecNumber evidence="5">6.3.5.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Formylglycinamide ribonucleotide amidotransferase subunit III</fullName>
            <shortName evidence="5">FGAR amidotransferase III</shortName>
            <shortName evidence="5">FGAR-AT III</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">Phosphoribosylformylglycinamidine synthase subunit III</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">purS</name>
        <name evidence="6" type="ordered locus">Metig_0341</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Part of the phosphoribosylformylglycinamidine synthase complex involved in the purines biosynthetic pathway. Catalyzes the ATP-dependent conversion of formylglycinamide ribonucleotide (FGAR) and glutamine to yield formylglycinamidine ribonucleotide (FGAM) and glutamate. The FGAM synthase complex is composed of three subunits. PurQ produces an ammonia molecule by converting glutamine to glutamate. PurL transfers the ammonia molecule to FGAR to form FGAM in an ATP-dependent manner. PurS interacts with PurQ and PurL and is thought to assist in the transfer of the ammonia molecule from PurQ to PurL.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>ATP + H2O + L-glutamine + N(2)-formyl-N(1)-(5-phospho-beta-D-ribosyl)glycinamide = 2-formamido-N(1)-(5-O-phospho-beta-D-ribosyl)acetamidine + ADP + H(+) + L-glutamate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:17129"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:147286"/>
            <dbReference type="ChEBI" id="CHEBI:147287"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.5.3"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="5">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Part of the FGAM synthase complex composed of 1 PurL, 1 PurQ and 2 PurS subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the PurS family.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.3" evidence="5"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95897.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798506.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0341"/>
    <dbReference type="EnsemblBacteria" id="AEF95897">
        <property type="protein sequence ID" value="AEF95897"/>
        <property type="gene ID" value="Metig_0341"/>
    </dbReference>
    <dbReference type="GeneID" id="10643178"/>
    <dbReference type="KEGG" id="mig:Metig_0341"/>
    <dbReference type="HOGENOM" id="CLU_164833_3_0_2"/>
    <dbReference type="OMA" id="PVIHDYE"/>
    <dbReference type="OrthoDB" id="117509at2157"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00128"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004642">
        <property type="term" value="F:phosphoribosylformylglycinamidine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1280.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01926">
        <property type="entry name" value="PurS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003850">
        <property type="entry name" value="PurS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036604">
        <property type="entry name" value="PurS-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34696">
        <property type="entry name" value="PTHR34696"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02700">
        <property type="entry name" value="PurS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82697">
        <property type="entry name" value="SSF82697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00302">
        <property type="entry name" value="TIGR00302"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1 5" id="KW-0436">Ligase</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3 5" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEF95897.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="83" mass="9634" checksum="AE5585C43FF7CF7A" modified="2011-07-27" version="1">MYKAIVTIKLKKGVLNPEGRTILRALNFLGYNEVKDAKTFKSIELLIEGDDEEKIKEKVDEMCRKLLANPVIHDYEIKLEKIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAU1</accession>
    <name>F6BAU1_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Xaa-Pro dipeptidase</fullName>
            <ecNumber evidence="5">3.4.13.9</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Metig_0349</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.4.13.9" evidence="5"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95905.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798514.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0349"/>
    <dbReference type="EnsemblBacteria" id="AEF95905">
        <property type="protein sequence ID" value="AEF95905"/>
        <property type="gene ID" value="Metig_0349"/>
    </dbReference>
    <dbReference type="GeneID" id="10643186"/>
    <dbReference type="KEGG" id="mig:Metig_0349"/>
    <dbReference type="HOGENOM" id="CLU_017266_4_2_2"/>
    <dbReference type="OMA" id="YCSDRTR"/>
    <dbReference type="OrthoDB" id="14436at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102009">
        <property type="term" value="F:proline dipeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.350.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029149">
        <property type="entry name" value="Creatin/AminoP/Spt16_NTD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036005">
        <property type="entry name" value="Creatinase/aminopeptidase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000587">
        <property type="entry name" value="Creatinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000994">
        <property type="entry name" value="Pept_M24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001714">
        <property type="entry name" value="Pept_M24_MAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001131">
        <property type="entry name" value="Peptidase_M24B_aminopep-P_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01321">
        <property type="entry name" value="Creatinase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00557">
        <property type="entry name" value="Peptidase_M24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00599">
        <property type="entry name" value="MAPEPTIDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53092">
        <property type="entry name" value="SSF53092"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55920">
        <property type="entry name" value="SSF55920"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00491">
        <property type="entry name" value="PROLINE_PEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0224">Dipeptidase</keyword>
    <keyword evidence="2 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0645">Protease</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Creatinase_N" evidence="4">
        <location>
            <begin position="6"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_M24" evidence="3">
        <location>
            <begin position="123"/>
            <end position="323"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEF95905.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="335" mass="38848" checksum="0D0ADA1AEAC10FB1" modified="2011-07-27" version="1">MDKIKVFVEYLHENEIKKAVILKKENINYFLERYPPNFSVLVFEGEEGILYVPKLDYEMAKKYEKDNLRVEIFEKWEDIFEGCDGIEETLPIKFLKHISKDYKIISDKIKEMRMIKNKSEIKLIKNAAEISDKAVKYIMENLENIGNKTENELAAEIEYVMKKNGSIRPSFDTIVISDKKTSYPHELPSNEIIKNILLVDIGAVYEGYCSDITRTFLLNPSKKMEEVYDIVSNAKKEVEKHLKEGVSAKELDEIAREFMGEYKEYFIHSLGHGVGVEVHESPTLSIKAKEDVVLKEGMVLTIEPGIYLKDEFGVRIEDLYLVKKNGFKKLSNAKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAW7</accession>
    <name>F6BAW7_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 4">GDP-mannose 4,6-dehydratase</fullName>
            <ecNumber evidence="3 4">4.2.1.47</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">GDP-D-mannose dehydratase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">gmd</name>
        <name evidence="6" type="ordered locus">Metig_1520</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Catalyzes the conversion of GDP-D-mannose to GDP-4-dehydro-6-deoxy-D-mannose.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>GDP-alpha-D-mannose = GDP-4-dehydro-alpha-D-rhamnose + H2O</text>
            <dbReference type="Rhea" id="RHEA:23820"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:57527"/>
            <dbReference type="ChEBI" id="CHEBI:57964"/>
            <dbReference type="EC" id="4.2.1.47"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 4">
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the NAD(P)-dependent epimerase/dehydratase family. GDP-mannose 4,6-dehydratase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.47" evidence="3 4"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97054.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799648.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1520"/>
    <dbReference type="EnsemblBacteria" id="AEF97054">
        <property type="protein sequence ID" value="AEF97054"/>
        <property type="gene ID" value="Metig_1520"/>
    </dbReference>
    <dbReference type="GeneID" id="10644393"/>
    <dbReference type="KEGG" id="mig:Metig_1520"/>
    <dbReference type="HOGENOM" id="CLU_007383_14_0_2"/>
    <dbReference type="OMA" id="TDCLYLG"/>
    <dbReference type="OrthoDB" id="43299at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008446">
        <property type="term" value="F:GDP-mannose 4,6-dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070401">
        <property type="term" value="F:NADP+ binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019673">
        <property type="term" value="P:GDP-mannose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00955">
        <property type="entry name" value="GDP_Man_dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006368">
        <property type="entry name" value="GDP_Man_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016040">
        <property type="entry name" value="NAD(P)-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43715">
        <property type="entry name" value="PTHR43715"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16363">
        <property type="entry name" value="GDP_Man_Dehyd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01472">
        <property type="entry name" value="gmd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-0521">NADP</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD(P)-bd_dom" evidence="5">
        <location>
            <begin position="5"/>
            <end position="330"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF16363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEF97054.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="344" mass="39382" checksum="12022263C27BF8A4" modified="2011-07-27" version="1">MKVALITGITGQDGYYLTKLLLEKGYEVHGIVRRNSKKSLGNLEHLSKEEKEQINIHWGDVTDSTFIDRTIKELKPDEVYHLAAQSFVGFSFENPKYTYDVNIGGTLNVVNAIKEYSPESKLYFAATSELYGKVQEIPQKETTPFYPRSPYAVSKLAGFWTVKNYRESYGLFMCNGILFNHESPMRGPEFVTRKISLGVAKIYYGLQKYIELGNLNAKRDWGFAGDYVYGMWLILQHHKPDDFVLATGETHSVREFVEKAFKVVDIEIEWEGKGVKEVGKNADTGEVLVKVNPKYFRPAEVDILVGDYSKAKKELGWEPKVKFDELVEMMVKADLKRIENKEII</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAX4</accession>
    <name>F6BAX4_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF1616 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_1527</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97061.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799655.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1527"/>
    <dbReference type="EnsemblBacteria" id="AEF97061">
        <property type="protein sequence ID" value="AEF97061"/>
        <property type="gene ID" value="Metig_1527"/>
    </dbReference>
    <dbReference type="GeneID" id="10644400"/>
    <dbReference type="KEGG" id="mig:Metig_1527"/>
    <dbReference type="HOGENOM" id="CLU_2032878_0_0_2"/>
    <dbReference type="OrthoDB" id="120862at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011674">
        <property type="entry name" value="DUF1616"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07760">
        <property type="entry name" value="DUF1616"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="33"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="68"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="97"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="DUF1616" evidence="2">
        <location>
            <begin position="14"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF97061.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="121" mass="14253" checksum="B6C2FC661974D0F6" modified="2011-07-27" version="1">MDKRLLIQNIDIIFILLCLIIVSYYMVFEKYNILRVIFGSLMVLFFPGYLLINTLFFNNKIFNNLEKFGLSLGLSICITGLLGFVLSLIYIISEYTILLTISLWNIFFSMLLFIMRAYNYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAZ5</accession>
    <name>F6BAZ5_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 7">Elongation factor 2</fullName>
            <shortName evidence="7">EF-2</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">fusA</name>
        <name evidence="9" type="ordered locus">Metig_1548</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6 7">Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-G/EF-2 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97082.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799676.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1548"/>
    <dbReference type="EnsemblBacteria" id="AEF97082">
        <property type="protein sequence ID" value="AEF97082"/>
        <property type="gene ID" value="Metig_1548"/>
    </dbReference>
    <dbReference type="GeneID" id="10644421"/>
    <dbReference type="KEGG" id="mig:Metig_1548"/>
    <dbReference type="HOGENOM" id="CLU_002794_11_1_2"/>
    <dbReference type="OMA" id="GVMTQTE"/>
    <dbReference type="OrthoDB" id="1642at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00054_A">
        <property type="entry name" value="EF_G_EF_2_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041095">
        <property type="entry name" value="EFG_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035647">
        <property type="entry name" value="EFG_III/V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000640">
        <property type="entry name" value="EFG_V-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031157">
        <property type="entry name" value="G_TR_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004543">
        <property type="entry name" value="Transl_elong_EFG/EF2_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005517">
        <property type="entry name" value="Transl_elong_EFG/EF2_IV"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00679">
        <property type="entry name" value="EFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14492">
        <property type="entry name" value="EFG_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03764">
        <property type="entry name" value="EFG_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00838">
        <property type="entry name" value="EFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00889">
        <property type="entry name" value="EFG_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="SSF50447"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54980">
        <property type="entry name" value="SSF54980"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00490">
        <property type="entry name" value="aEF-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00301">
        <property type="entry name" value="G_TR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 9" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="5 7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4 7" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="8">
        <location>
            <begin position="19"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="28"/>
            <end position="35"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="94"/>
            <end position="98"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="148"/>
            <end position="151"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="Diphthamide" evidence="7">
        <location>
            <position position="603"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00017891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AEF97082.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="727" mass="81018" checksum="2DE7206DECE32CF9" modified="2011-07-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAZ7</accession>
    <name>F6BAZ7_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">30S ribosomal protein S10</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">rps10</name>
        <name evidence="6" type="ordered locus">Metig_1550</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Involved in the binding of tRNA to the ribosomes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Part of the 30S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the universal ribosomal protein uS10 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97084.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799678.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1550"/>
    <dbReference type="EnsemblBacteria" id="AEF97084">
        <property type="protein sequence ID" value="AEF97084"/>
        <property type="gene ID" value="Metig_1550"/>
    </dbReference>
    <dbReference type="GeneID" id="10644423"/>
    <dbReference type="KEGG" id="mig:Metig_1550"/>
    <dbReference type="HOGENOM" id="CLU_122625_0_1_2"/>
    <dbReference type="OMA" id="QFEIRVH"/>
    <dbReference type="OrthoDB" id="107993at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.600">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00508">
        <property type="entry name" value="Ribosomal_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001848">
        <property type="entry name" value="Ribosomal_S10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018268">
        <property type="entry name" value="Ribosomal_S10_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027486">
        <property type="entry name" value="Ribosomal_S10_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036838">
        <property type="entry name" value="Ribosomal_S10_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005729">
        <property type="entry name" value="Ribosomal_S10_euk/arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11700">
        <property type="entry name" value="PTHR11700"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00338">
        <property type="entry name" value="Ribosomal_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00971">
        <property type="entry name" value="RIBOSOMALS10"/>
    </dbReference>
    <dbReference type="SMART" id="SM01403">
        <property type="entry name" value="Ribosomal_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54999">
        <property type="entry name" value="SSF54999"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01046">
        <property type="entry name" value="uS10_euk_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00361">
        <property type="entry name" value="RIBOSOMAL_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 6" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Ribosomal_S10" evidence="5">
        <location>
            <begin position="5"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM01403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEF97084.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="101" mass="11637" checksum="E756014032124741" modified="2011-07-27" version="1">MQKARIKLSGTNHKELDEVCRQIKAIAEKTGVDISGPIPLPTKVLKVVTRKAPDGEGSETYERWYMKIHKRLIDIEADERTLRHIMKVKIPDSVQIEIQFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F6BB11</accession>
    <name>F6BB11_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="9">AzlC family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Metig_1564</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the AzlC family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97098.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799692.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1564"/>
    <dbReference type="EnsemblBacteria" id="AEF97098">
        <property type="protein sequence ID" value="AEF97098"/>
        <property type="gene ID" value="Metig_1564"/>
    </dbReference>
    <dbReference type="GeneID" id="10644437"/>
    <dbReference type="KEGG" id="mig:Metig_1564"/>
    <dbReference type="HOGENOM" id="CLU_065777_3_1_2"/>
    <dbReference type="OMA" id="IVENKMG"/>
    <dbReference type="OrthoDB" id="72210at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011606">
        <property type="entry name" value="Brnchd-chn_aa_trnsp_permease"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34979">
        <property type="entry name" value="PTHR34979"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03591">
        <property type="entry name" value="AzlC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="6"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="37"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="64"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="116"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="147"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="171"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010735"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AEF97098.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="210" mass="22594" checksum="D77CF63BF32B65B0" modified="2011-07-27" version="1">MFKRGLIGSFPIAIGYLPVAITFGVSALALGFSKVEILLMSCLIFAGASQFALISLLPYSVIDAVSVALILNLRHIIYGYLISQKFELKKQFITAFGLTDEVFASSIVSKEKDEKYILGLEVGSYLSWILGTLVGIFGGDILLSNEIIAPSLLFSLTALYFILLIPNIKKLGSSAIVGGVVALVFYYFGYPSIGILVAGILSPLVVLKIK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-02-22" modified="2022-05-25" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>F6BB32</accession>
    <name>HPRT_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Hypoxanthine/guanine phosphoribosyltransferase</fullName>
            <shortName evidence="1">HGPRTase</shortName>
            <ecNumber evidence="1">2.4.2.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">hpt</name>
        <name type="ordered locus">Metig_0361</name>
    </gene>
    <organism>
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes a salvage reaction resulting in the formation of IMP that is energically less costly than de novo synthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>diphosphate + IMP = 5-phospho-alpha-D-ribose 1-diphosphate + hypoxanthine</text>
            <dbReference type="Rhea" id="RHEA:17973"/>
            <dbReference type="ChEBI" id="CHEBI:17368"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <dbReference type="EC" id="2.4.2.8"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>diphosphate + GMP = 5-phospho-alpha-D-ribose 1-diphosphate + guanine</text>
            <dbReference type="Rhea" id="RHEA:25424"/>
            <dbReference type="ChEBI" id="CHEBI:16235"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
            <dbReference type="EC" id="2.4.2.8"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Purine metabolism; IMP biosynthesis via salvage pathway; IMP from hypoxanthine: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the purine/pyrimidine phosphoribosyltransferase family. Archaeal HPRT subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.8" evidence="1"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95917.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798526.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6BB32"/>
    <dbReference type="SMR" id="F6BB32"/>
    <dbReference type="STRING" id="880724.Metig_0361"/>
    <dbReference type="EnsemblBacteria" id="AEF95917">
        <property type="protein sequence ID" value="AEF95917"/>
        <property type="gene ID" value="Metig_0361"/>
    </dbReference>
    <dbReference type="GeneID" id="10643198"/>
    <dbReference type="KEGG" id="mig:Metig_0361"/>
    <dbReference type="HOGENOM" id="CLU_126376_0_0_2"/>
    <dbReference type="OMA" id="FIHPISD"/>
    <dbReference type="OrthoDB" id="88302at2157"/>
    <dbReference type="UniPathway" id="UPA00591">
        <property type="reaction ID" value="UER00648"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052657">
        <property type="term" value="F:guanine phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004422">
        <property type="term" value="F:hypoxanthine phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043103">
        <property type="term" value="P:hypoxanthine salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032264">
        <property type="term" value="P:IMP salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006166">
        <property type="term" value="P:purine ribonucleoside salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01467">
        <property type="entry name" value="Hypx_phosphoribosyltr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026597">
        <property type="entry name" value="HGPRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRibTrfase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00156">
        <property type="entry name" value="Pribosyltran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="SSF53271"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00103">
        <property type="entry name" value="PUR_PYR_PR_TRANSFER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0328">Glycosyltransferase</keyword>
    <keyword id="KW-0660">Purine salvage</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000415487" description="Hypoxanthine/guanine phosphoribosyltransferase">
        <location>
            <begin position="1"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01467"/>
        </source>
    </evidence>
    <sequence length="183" mass="20417" checksum="9E0CCCCABC9694A1" modified="2011-07-27" version="1">MLLEETLKSCPTVKRGDYEYFIHPISDGVPIVNPKLLREVATRIIKEVDFENIDKIVTAEAMGIPLVTTLSLYTDVPYVIMRKREYKLKGEIPVHQETGYSKGQLYLNGIEKGDRVLIVDDVISTGGTMIAIINALKKAGAEIKDIVCVIERGEGKKIVEEKTGYKIKTLVKLDVKDGKVVIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>F6BB55</accession>
    <name>F6BB55_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Dihydroorotase</fullName>
            <shortName evidence="3">DHOase</shortName>
            <ecNumber evidence="3">3.5.2.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">pyrC</name>
        <name evidence="5" type="ordered locus">Metig_0384</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the reversible cyclization of carbamoyl aspartate to dihydroorotate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>(S)-dihydroorotate + H2O = H(+) + N-carbamoyl-L-aspartate</text>
            <dbReference type="Rhea" id="RHEA:24296"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30864"/>
            <dbReference type="ChEBI" id="CHEBI:32814"/>
            <dbReference type="EC" id="3.5.2.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="3">Binds 2 Zn(2+) ions per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 3/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the metallo-dependent hydrolases superfamily. DHOase family. Class I DHOase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.5.2.3" evidence="3"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95940.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798549.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0384"/>
    <dbReference type="EnsemblBacteria" id="AEF95940">
        <property type="protein sequence ID" value="AEF95940"/>
        <property type="gene ID" value="Metig_0384"/>
    </dbReference>
    <dbReference type="GeneID" id="10643221"/>
    <dbReference type="KEGG" id="mig:Metig_0384"/>
    <dbReference type="HOGENOM" id="CLU_015572_1_1_2"/>
    <dbReference type="OMA" id="QHAQEPR"/>
    <dbReference type="OrthoDB" id="43469at2157"/>
    <dbReference type="UniPathway" id="UPA00070">
        <property type="reaction ID" value="UER00117"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004151">
        <property type="term" value="F:dihydroorotase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044205">
        <property type="term" value="P:'de novo' UMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00220_A">
        <property type="entry name" value="PyrC_classI_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006680">
        <property type="entry name" value="Amidohydro-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004722">
        <property type="entry name" value="DHOase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002195">
        <property type="entry name" value="Dihydroorotase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011059">
        <property type="entry name" value="Metal-dep_hydrolase_composite"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01979">
        <property type="entry name" value="Amidohydro_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51338">
        <property type="entry name" value="SSF51338"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="SSF51556"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00482">
        <property type="entry name" value="DIHYDROOROTASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00483">
        <property type="entry name" value="DIHYDROOROTASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Amidohydro-rel" evidence="4">
        <location>
            <begin position="48"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="3">
        <location>
            <begin position="59"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="active site" evidence="3">
        <location>
            <position position="303"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="57"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="59"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="90"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="138"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="169"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="228"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="303"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="307"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="modified residue" description="N6-carboxylysine" evidence="3">
        <location>
            <position position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEF95940.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="424" mass="48330" checksum="A84B8A7B0374CE68" modified="2011-07-27" version="1">MLLKNCRIIKNNKIIEGDILIDEENGRIEKIRKSIPYNGETIDLKNKIVISGVIDAHVHFRYGEEKKEDFISGSEAAINGGVCFAIDMPNNKPPITTKELFYKKYKEGKEKSKINIEFNFGVTENNYLETVEDAKAYKIFMVKSVGDLFISDYSKLKDILNQNKLFCIHAEHKDIIEENLKRCSLNSWTDHCKIRDKKSEVEAIKEIIKNLKIIDKIGKYKPHIHFCHVSTKEGLYLIKKAREELKNVKITVEVTPHHLYLNMDMAEDLKGLGKFNPPLRTKEDNIALIKGIVNKDVDIIATDHAPHIYEEKSNEPKNCPSGIPGIETFVPLVFNLVSKKLISLFDAAKMLSENPAKIFNIDNEIREGNLANLTIVDLDMEGEIRGDNFKSKAKFTPFEGWKVKGLPIYTVVNGSLYDAYGKII</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>F6BB57</accession>
    <name>F6BB57_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0386</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95942.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798551.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0386"/>
    <dbReference type="EnsemblBacteria" id="AEF95942">
        <property type="protein sequence ID" value="AEF95942"/>
        <property type="gene ID" value="Metig_0386"/>
    </dbReference>
    <dbReference type="GeneID" id="10643223"/>
    <dbReference type="KEGG" id="mig:Metig_0386"/>
    <dbReference type="HOGENOM" id="CLU_104519_0_0_2"/>
    <dbReference type="OMA" id="IFGHKMA"/>
    <dbReference type="OrthoDB" id="93562at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019206">
        <property type="entry name" value="DUF2085_TM"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09858">
        <property type="entry name" value="DUF2085"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="66"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="101"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="128"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF95942.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="155" mass="17862" checksum="71F8E5593954E536" modified="2011-07-27" version="1">MLKKFYATILISFIIFYAGIFLAPYTAYLGEGSEVFRWVSFFIYTIYSTICHQLPQRSYFIFGHKMAVCARCFGIYTGVLIGMLIYPFIKRLEDFKTPSKWYLIIALTPMAIDGTTQLIGLRESFNELRFITGFIAGFVGVFYILPVFLKILSKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>F6BB64</accession>
    <name>F6BB64_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Putative methylthioribose-1-phosphate isomerase</fullName>
            <shortName evidence="2">M1Pi</shortName>
            <shortName evidence="2">MTR-1-P isomerase</shortName>
            <ecNumber evidence="2">5.3.1.23</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">MTNA-like protein</fullName>
            <shortName evidence="2">aMTNA</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="2">S-methyl-5-thioribose-1-phosphate isomerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_0393</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the interconversion of methylthioribose-1-phosphate (MTR-1-P) into methylthioribulose-1-phosphate (MTRu-1-P).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>S-methyl-5-thio-alpha-D-ribose 1-phosphate = S-methyl-5-thio-D-ribulose 1-phosphate</text>
            <dbReference type="Rhea" id="RHEA:19989"/>
            <dbReference type="ChEBI" id="CHEBI:58533"/>
            <dbReference type="ChEBI" id="CHEBI:58548"/>
            <dbReference type="EC" id="5.3.1.23"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the EIF-2B alpha/beta/delta subunits family. MtnA subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.23" evidence="2"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95949.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0393"/>
    <dbReference type="EnsemblBacteria" id="AEF95949">
        <property type="protein sequence ID" value="AEF95949"/>
        <property type="gene ID" value="Metig_0393"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0393"/>
    <dbReference type="HOGENOM" id="CLU_016218_1_2_2"/>
    <dbReference type="OMA" id="RLWVDET"/>
    <dbReference type="OrthoDB" id="54988at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046523">
        <property type="term" value="F:S-methyl-5-thioribose-1-phosphate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019284">
        <property type="term" value="P:L-methionine salvage from S-adenosylmethionine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10470">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01678">
        <property type="entry name" value="Salvage_MtnA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000649">
        <property type="entry name" value="IF-2B-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005251">
        <property type="entry name" value="IF-M1Pi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042529">
        <property type="entry name" value="IF_2B-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011559">
        <property type="entry name" value="Initiation_fac_2B_a/b/d"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027363">
        <property type="entry name" value="M1Pi_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01008">
        <property type="entry name" value="IF-2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="SSF100950"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00524">
        <property type="entry name" value="eIF-2B_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00512">
        <property type="entry name" value="salvage_mtnA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="1 2 3" id="KW-0413">Isomerase</keyword>
    <keyword evidence="2" id="KW-0486">Methionine biosynthesis</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Substrate binding" evidence="2">
        <location>
            <begin position="55"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="2">
        <location>
            <begin position="238"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="2">
        <location>
            <position position="228"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="89"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="187"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="2">
        <location>
            <position position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF95949.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="328" mass="36869" checksum="ACE7A446D382A0E3" modified="2011-07-27" version="1">MVKKMRGKDLRPIIWDDDKKELILIDQRKLPHKLEYFTCKTYEDVAFAIKDMVVRGAPAIGISAAYGMALAEIHGEDIEKAYNTLKNTRPTAVNLFWALNRVMDAYDKGRSILEEAKKIHEEDIETCRKIGEIGEKLIEDGDTILTHCNAGALACSAYGTALSVIRFAHYNNKKIQVIADETRPRLQGAKLTAFELSYEGIPVKVIPDNMAGYLMSKGMIDKIIVGADRILSDYHVFNKIGTYSLAILAKYHNIPFYVAAPYSTFDFESKVEDVIVEERGEKEVAYIDGVRIIPEGVGVYNYAFDCTPPELITAIITEKGIIYPNEKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>F6BB80</accession>
    <name>F6BB80_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_0409</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95965.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF95965">
        <property type="protein sequence ID" value="AEF95965"/>
        <property type="gene ID" value="Metig_0409"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0409"/>
    <dbReference type="HOGENOM" id="CLU_212436_0_0_2"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="SSF57903"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF95965.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="53" mass="6360" checksum="9C37DBC1CA88F877" modified="2011-07-27" version="1">MLNNIEELDISKYVTIDLDALKTKTYKCPFCNKEFKYVGKKMMCPYCKRMIKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBB0</accession>
    <name>F6BBB0_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Putative asparagine synthetase [glutamine-hydrolyzing]</fullName>
            <ecNumber evidence="4">6.3.5.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Metig_1584</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>ATP + H2O + L-aspartate + L-glutamine = AMP + diphosphate + H(+) + L-asparagine + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:12228"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58048"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.5.4"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="6.3.5.4" evidence="4"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97117.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799710.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1584"/>
    <dbReference type="MEROPS" id="C44.001"/>
    <dbReference type="EnsemblBacteria" id="AEF97117">
        <property type="protein sequence ID" value="AEF97117"/>
        <property type="gene ID" value="Metig_1584"/>
    </dbReference>
    <dbReference type="GeneID" id="10644457"/>
    <dbReference type="KEGG" id="mig:Metig_1584"/>
    <dbReference type="HOGENOM" id="CLU_014658_4_0_2"/>
    <dbReference type="OMA" id="DWSGIYS"/>
    <dbReference type="OrthoDB" id="12911at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004066">
        <property type="term" value="F:asparagine synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006529">
        <property type="term" value="P:asparagine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006541">
        <property type="term" value="P:glutamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01991">
        <property type="entry name" value="Asn_Synthase_B_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00712">
        <property type="entry name" value="AsnB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006426">
        <property type="entry name" value="Asn_synth_AEB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001962">
        <property type="entry name" value="Asn_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033738">
        <property type="entry name" value="AsnB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017932">
        <property type="entry name" value="GATase_2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00733">
        <property type="entry name" value="Asn_synthase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001589">
        <property type="entry name" value="Asn_synthetase_glu-h"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="SSF56235"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01536">
        <property type="entry name" value="asn_synth_AEB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51278">
        <property type="entry name" value="GATASE_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="5" id="KW-0061">Asparagine biosynthesis</keyword>
    <keyword evidence="2 4 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 5" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="8" id="KW-0436">Ligase</keyword>
    <keyword evidence="1 4 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutamine amidotransferase type-2" evidence="7">
        <location>
            <begin position="2"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="active site" description="For GATase activity" evidence="5">
        <location>
            <position position="2"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="118"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="291"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="365"/>
            <end position="366"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR001589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR001589-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR001589-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEF97117.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="511" mass="59460" checksum="FA70026FEF225088" modified="2011-07-27" version="1">MCSISGIIIKDEEDPRSLRRDISKYLLTMMKILKHRGPDASGMLVDDEVIYFKDFEDVEKSIIADFGFGHNRLAIVGSATQPIPNEDESLWVICNGEIYNYIELREDLSNHDFKTDTDSEVIVHAYEEDMLNELDGDYAFAIYDKNNSTLILRRDTFGVKPLFYLDRDGYFAFASERKALWYLLREIEGIDGSPKHLNEYINRLSPNSELIYYLEDNKYTITNNIEKLRFDYLKYKSYETCKKELDNALWRAVWKRTRGIERVGIIYSGGVDSTLVAKMASEYCEVILYTVGTEESEDIRYAERAARDMGLKLRKKIIKEEEYERYIFEVAKAIDELDLMKIGVGIPIYIASEMAREDNIKVVLSGQGADELFGGYSRYERILREKGEEELKKALLNDVMNIYKVNLERDDHCTMANSVELRVPFLDKDVVRVALSIPVTYKMDEMRKRILRDIASKYIPDYIAYRPKKAAQYGSGSEKMIFKVARKYGYSKKEVNKFFEEVVLKKINETL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-02-10" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBD1</accession>
    <name>F6BBD1_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Integrase catalytic region</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_1605</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97138.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF97138">
        <property type="protein sequence ID" value="AEF97138"/>
        <property type="gene ID" value="Metig_1605"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_1605"/>
    <dbReference type="HOGENOM" id="CLU_079271_0_0_2"/>
    <dbReference type="OMA" id="ENWIIAF"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00665">
        <property type="entry name" value="rve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="2">
        <location>
            <begin position="151"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF97138.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="314" mass="37293" checksum="7D1FF95D88536A21" modified="2011-07-27" version="1">MVSRNNFASVGNKKRNVNNKNNMKGAKLNDKMIRKIIRWKEKGFETSRIAKKVGVTPRRVQQIWKEYRETGEIPKIKRRGRKPKKISKEEIDLVLKAVEEYKGTSAVHLEKYIENKHNIHIPHNRIYTILKEFDLTHKRKKKRKKKPKRFSASKPNEMWQMDFKIVDVDGVRYNLLLIIDDHSRFILHAGIYEEATTDVVISALEECFEKHGTPKKILTDNGTQFVPARGGISRFQKFLMEREILHIKTSIRHPQTIGKVERINREVEYRLDKFNNLKEFVEWHNQIKPHRSLSFKTPYEVYFENTKCNKEVIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBE0</accession>
    <name>F6BBE0_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(NiFe)-hydrogenase-3-type complex Eha, membrane protein EhaF</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1614</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97147.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799740.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1614"/>
    <dbReference type="EnsemblBacteria" id="AEF97147">
        <property type="protein sequence ID" value="AEF97147"/>
        <property type="gene ID" value="Metig_1614"/>
    </dbReference>
    <dbReference type="GeneID" id="10644487"/>
    <dbReference type="KEGG" id="mig:Metig_1614"/>
    <dbReference type="HOGENOM" id="CLU_1639963_0_0_2"/>
    <dbReference type="OMA" id="YTRGMDT"/>
    <dbReference type="OrthoDB" id="74022at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011313">
        <property type="entry name" value="Prd_NiFe_hyd_3_EhaF"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09879">
        <property type="entry name" value="DUF2106"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF019373">
        <property type="entry name" value="EhaF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="132"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF97147.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="154" mass="17486" checksum="9B7BBC85E475D926" modified="2011-07-27" version="1">MNKLSKIWNYLSKPHVVPRIFAGFLALVMIFGLLLPHELNKNQLYPKPVPQSQVLKTPLAPYDRGGIPLEKPAEIISQYPQFEPLLGKITAYLTPIAEWISKKTLYFGTTIVSTPGGILDEILYYTRGFDTILESTILLVSFIIFSWMLMNRDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBF8</accession>
    <name>F6BBF8_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">S-layer protein, C-terminal</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1632</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97165.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799757.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF97165">
        <property type="protein sequence ID" value="AEF97165"/>
        <property type="gene ID" value="Metig_1632"/>
    </dbReference>
    <dbReference type="GeneID" id="10644505"/>
    <dbReference type="KEGG" id="mig:Metig_1632"/>
    <dbReference type="HOGENOM" id="CLU_640322_0_0_2"/>
    <dbReference type="OMA" id="CVLALNQ"/>
    <dbReference type="OrthoDB" id="26454at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022651">
        <property type="entry name" value="S_layer_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006454">
        <property type="entry name" value="S_layer_MJ"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05124">
        <property type="entry name" value="S_layer_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01564">
        <property type="entry name" value="S_layer_MJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="S_layer_C" evidence="1">
        <location>
            <begin position="327"/>
            <end position="408"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF97165.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="428" mass="49405" checksum="6A240FA4D49E9902" modified="2011-07-27" version="1">MDIKKVGILFLMFIFLSFCSAFPTNAIVIANKDSVDYDYAKLLMNNMYSNKKVTVVGNHINITDTKVYYIPAYNKLKISENGETLIVEFENNNKSIQYKSLTYEREINFEKDKKVEFFGKLYKILDHDGDSLILGDEVKNITTNESFEYKNYKIVLKAVSFDKNELLVDVYKDNELIESVKLKKGKYHQVKDSDIGIIYNETYESLKRLYFNFNVYDAIKLVDGEEFPLNKDFIVYIRGNKIELEHKHPENLPENFKIMNFNVYVDSFGNITTFRVKTTHNYEEEIDDIQYLGDDVWGIKEGNKTYVFYCGEKYKNATFYFGCGKILDDNVLKVDSDLILIGGPVSNNITNQIKDKLKIKIDNNNPGKNRGVIQLIKNPYNPKHNILVLAGSDRNGTKACVLAVINGIYKNQDIMIVELDGDKVKVIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBH8</accession>
    <name>F6BBH8_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Type II site-specific deoxyribonuclease</fullName>
            <ecNumber evidence="1">3.1.21.4</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_1653</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.1.21.4" evidence="1"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97185.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799776.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1653"/>
    <dbReference type="REBASE" id="36262">
        <property type="entry name" value="Mig5ORF1652P"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF97185">
        <property type="protein sequence ID" value="AEF97185"/>
        <property type="gene ID" value="Metig_1653"/>
    </dbReference>
    <dbReference type="GeneID" id="10644526"/>
    <dbReference type="KEGG" id="mig:Metig_1653"/>
    <dbReference type="HOGENOM" id="CLU_756000_0_0_2"/>
    <dbReference type="OMA" id="PNAGEMR"/>
    <dbReference type="OrthoDB" id="58569at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009036">
        <property type="term" value="F:type II site-specific deoxyribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009307">
        <property type="term" value="P:DNA restriction-modification system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019045">
        <property type="entry name" value="Restrct_endonuc_II_TdeIII"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09520">
        <property type="entry name" value="RE_TdeIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF97185.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="371" mass="43812" checksum="434B0D4015DFFA6B" modified="2011-07-27" version="1">MLPEDKIEEIAIETIRVLVNRFRSLPDENSELRNMPFHLAFLKAFYKKIDIKNEDEAIRFLTLSSWFHGLSTTLGQSYFENVAHILSDGEKRTFNNYSIPKSAKMIIEDIITDLKSGSRKPNMKEEEKMLRRALFEKGNTNELERGLNFTADVYIETKEEVIMIELKSVRPNAGEMRGEKQKILYGKAYLMYQFPKKKVRYYLGFPFDPTSNNDPCGYDKERFASYLIEFSKYFDKDEFLIAGELWDFLSGEKDTMCEILKIINDIATPKFEEEFKIVNRFCFINEDALYTENAIDVKKFEKYIEILNKWKLYSEIELAKIVSKIGIKEIPNPYRKQFEKLINLSMFDDTGRYNKKRKEKLMGLYDKLEIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBI6</accession>
    <name>F6BBI6_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_0439</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95995.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF95995">
        <property type="protein sequence ID" value="AEF95995"/>
        <property type="gene ID" value="Metig_0439"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0439"/>
    <dbReference type="HOGENOM" id="CLU_810409_0_0_2"/>
    <dbReference type="OMA" id="WIDNRIE"/>
    <dbReference type="OrthoDB" id="76698at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF95995.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="342" mass="38757" checksum="E2B7851BEB41DDCF" modified="2011-07-27" version="1">MNLTMLRKCFTILLAILLLASPVSAFFSFDFSISQNRAVAGATIAAETIADAMVSNYIEENHPEYSDAYTVVSLIFDPTGKIIGKLSSKGLKYADNLYRYLKKADNGKGLVNPDVANKIFDKDLIEKLAEKENKGYGPKTVAKNTVKLLEEGIKPENINKLFKDAVENDKKVGWIDNRIETLEENEVDIDTINDFMEKSSNPYDDLRHLNCKVATLVALGISKDNLNKIIKNSKNIKDIYNCKEDLKKLKKMGITKENLNKIIENLKDIKDITKIRKTIGDLKRRKISKESINKLIENGYDLEKVKEIIKELRNKGVPSKIIDGLIKKEYSLKEISAFNKFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBK3</accession>
    <name>F6BBK3_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ATPase associated with various cellular activities AAA_5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0456</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96012.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798621.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0456"/>
    <dbReference type="EnsemblBacteria" id="AEF96012">
        <property type="protein sequence ID" value="AEF96012"/>
        <property type="gene ID" value="Metig_0456"/>
    </dbReference>
    <dbReference type="GeneID" id="10643293"/>
    <dbReference type="KEGG" id="mig:Metig_0456"/>
    <dbReference type="HOGENOM" id="CLU_018678_0_1_2"/>
    <dbReference type="OMA" id="TRFTEPD"/>
    <dbReference type="OrthoDB" id="43030at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001270">
        <property type="entry name" value="ClpA/B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045427">
        <property type="entry name" value="MoxR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041538">
        <property type="entry name" value="RavA-like_AAA_lid"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17868">
        <property type="entry name" value="AAA_lid_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20030">
        <property type="entry name" value="bpMoxR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00300">
        <property type="entry name" value="CLPPROTEASEA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA" evidence="1">
        <location>
            <begin position="30"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96012.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="374" mass="43531" checksum="89BC4F3B436F549A" modified="2011-07-27" version="1">MELQEIRRELNDYFLERREEIDIALTALIANEHCIFLGNPGVAKSQLIRAIASHINANYFEKLITRFSTEDELFGPLSIKELKENDRFVRKTENYLPNAEIAFLDEIFKANSSILNALLSIMNERIYHNGDVIQKVPLISLFGASNELPEENELLAFYDRFLFRKKVDRIKSMLNLEKLIDLNENYKPKTTMDIDEIKKLREKIEKVDISNIKRYLIDIKMTLENEGIYISDRRFKKSVKAVKAYAFLNGKDNADENDLDILRHIYWDEPNEFYKVSITIFKISNHFSGFALEQREILDALMGELKKINKDRLPLGGIEYRKVLEILGKINSIAITLKDVKRKAEENGKPSEFVEEVLKEAEGLKNYVEGLLKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBM5</accession>
    <name>F6BBM5_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">tRNA(Ile2) 2-agmatinylcytidine synthetase TiaS</fullName>
            <shortName evidence="1">tRNA(Ile2)-agm2C synthetase</shortName>
            <ecNumber evidence="1">6.3.4.22</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">tRNA(Ile2) agmatidine synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">tiaS</name>
        <name evidence="4" type="ordered locus">Metig_0478</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">ATP-dependent agmatine transferase that catalyzes the formation of 2-agmatinylcytidine (agm2C) at the wobble position (C34) of tRNA(Ile2), converting the codon specificity from AUG to AUA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>agmatine + ATP + cytidine(34) in tRNA(Ile2) + H2O = 2-agmatinylcytidine(34) in tRNA(Ile2) + AMP + 2 H(+) + 2 phosphate</text>
            <dbReference type="Rhea" id="RHEA:43608"/>
            <dbReference type="Rhea" id="RHEA-COMP:10625"/>
            <dbReference type="Rhea" id="RHEA-COMP:10626"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58145"/>
            <dbReference type="ChEBI" id="CHEBI:82748"/>
            <dbReference type="ChEBI" id="CHEBI:83545"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.4.22"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TiaS family.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.22" evidence="1"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96034.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798642.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0478"/>
    <dbReference type="EnsemblBacteria" id="AEF96034">
        <property type="protein sequence ID" value="AEF96034"/>
        <property type="gene ID" value="Metig_0478"/>
    </dbReference>
    <dbReference type="GeneID" id="10643315"/>
    <dbReference type="KEGG" id="mig:Metig_0478"/>
    <dbReference type="HOGENOM" id="CLU_675459_0_0_2"/>
    <dbReference type="OMA" id="GMCTTYL"/>
    <dbReference type="OrthoDB" id="58019at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016879">
        <property type="term" value="F:ligase activity, forming carbon-nitrogen bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002101">
        <property type="term" value="P:tRNA wobble cytosine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01892">
        <property type="entry name" value="tRNA_Ile2_agm2C_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013696">
        <property type="entry name" value="DUF1743"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004365">
        <property type="entry name" value="NA-bd_OB_tRNA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024913">
        <property type="entry name" value="tRNA_Ile2__agm2C_synt"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08489">
        <property type="entry name" value="DUF1743"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01336">
        <property type="entry name" value="tRNA_anti-codon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1" id="KW-0436">Ligase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="DUF1743" evidence="3">
        <location>
            <begin position="147"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="domain" description="OB" evidence="2">
        <location>
            <begin position="272"/>
            <end position="346"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF08489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEF96034.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="418" mass="48266" checksum="0EA0277484E8B7E3" modified="2011-07-27" version="1">MFIGIDDTDSPNKYCTTYIATLLVEKFKEMGYNVEMPKLIRMNPTVKYKTRGNGGVSIRIIEDKKLSDNEKREIKDIVIEYVENYADFEYETTNPGIVFLDEENYAKNKDLLNSYYKKVLYDIVSIDYVESILKKINAEYIKYKKGYGIIGALGAISSNPPYTYELLAYRKRENWGKERFVDEKSVFEMDEKTFPFTFNNVDYDEGKSIIAPHTKCPVLYGIRGIDKDILIEAMKMIKSEEIDRYMIFKTNQGTDVHLRYMKIKDIYPNTGVIVYGKVVKEPKNLPGGHVVFRVSDGTGEIDCIAYEPTKKFRNIVRKLIVGDLVGVYGTVREKPFEINIEKLKIIKLAKKYTKDKKCECGGTLKAKGKKGGYKCRKCGKVIKYDEIKLIEVERDITEGFYEVPPSARRHLSKPILLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBN3</accession>
    <name>F6BBN3_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Ureidoglycolate lyase</fullName>
            <ecNumber evidence="2">4.3.2.3</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0486</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EC" id="4.3.2.3" evidence="2"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96042.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0486"/>
    <dbReference type="EnsemblBacteria" id="AEF96042">
        <property type="protein sequence ID" value="AEF96042"/>
        <property type="gene ID" value="Metig_0486"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0486"/>
    <dbReference type="HOGENOM" id="CLU_028458_4_2_2"/>
    <dbReference type="OMA" id="PPEPIIF"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050385">
        <property type="term" value="F:ureidoglycolate lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.850.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011234">
        <property type="entry name" value="Fumarylacetoacetase-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036663">
        <property type="entry name" value="Fumarylacetoacetase_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01557">
        <property type="entry name" value="FAA_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56529">
        <property type="entry name" value="SSF56529"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAA_hydrolase" evidence="1">
        <location>
            <begin position="34"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96042.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="231" mass="26301" checksum="F59FFB61C89848A4" modified="2011-07-27" version="1">MDSIIFCRTTILKSKFNKMKMVKYMDIKNIKPTKIICVGLNYIDHAKELNMEIPEEPIIFMKPPSSIIYHEDTIIRPKISKRVDYEVELAVVIGKKGKNIKKNEADNYILGYTILNDVTARDLQAKDGQWTRAKSFDTFCPIGPRIVKDIDPMNLNIECRVNGEIKQKSNTKNMIFDVYELIEFVSSIMTLYPGDIISTGTPPGVGELKKGDVVECEIEGIGVLRNYVDDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-06-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBQ5</accession>
    <name>F6BBQ5_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_0508</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96064.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798672.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96064">
        <property type="protein sequence ID" value="AEF96064"/>
        <property type="gene ID" value="Metig_0508"/>
    </dbReference>
    <dbReference type="GeneID" id="10643345"/>
    <dbReference type="KEGG" id="mig:Metig_0508"/>
    <dbReference type="HOGENOM" id="CLU_1965564_0_0_2"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF96064.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="127" mass="14351" checksum="75051179BB6B5DB6" modified="2011-07-27" version="1">MKVIINIKCPKDDADGTHWEDYTIEITEGGGSFPVTYANCKIVSAYVPVICCDCGERFDAEVEINEGEKVAQKVKDMDQEILWPISYEGNCPNCGNPVEFIIDMWEYPQGLIETLVKDYSSNVKFIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-09-29" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBR7</accession>
    <name>F6BBR7_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="5">DNA binding domain protein, excisionase family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Metig_1665</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97197.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF97197">
        <property type="protein sequence ID" value="AEF97197"/>
        <property type="gene ID" value="Metig_1665"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_1665"/>
    <dbReference type="HOGENOM" id="CLU_082093_0_1_2"/>
    <dbReference type="OMA" id="QWATERD"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000150">
        <property type="term" value="F:DNA strand exchange activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03769">
        <property type="entry name" value="SR_IS607_transposase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1390">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009061">
        <property type="entry name" value="DNA-bd_dom_put_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041657">
        <property type="entry name" value="HTH_17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041718">
        <property type="entry name" value="IS607_transposase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006118">
        <property type="entry name" value="Recombinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006119">
        <property type="entry name" value="Resolv_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036162">
        <property type="entry name" value="Resolvase-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010093">
        <property type="entry name" value="SinI_DNA-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12728">
        <property type="entry name" value="HTH_17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00239">
        <property type="entry name" value="Resolvase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00857">
        <property type="entry name" value="Resolvase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46955">
        <property type="entry name" value="SSF46955"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53041">
        <property type="entry name" value="SSF53041"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01764">
        <property type="entry name" value="excise"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00397">
        <property type="entry name" value="RECOMBINASES_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51736">
        <property type="entry name" value="RECOMBINASES_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0229">DNA integration</keyword>
    <keyword evidence="3" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Resolvase/invertase-type recombinase catalytic" evidence="4">
        <location>
            <begin position="62"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEF97197.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="209" mass="24488" checksum="928D17F5C5F38973" modified="2011-07-27" version="1">MSLERLYTMKEACELLGVHIKTLQRWDREGKIKCVRTVGGKRRVPESEIKRILGIKDKEQRKIIGYARVSSNTQKDDLERQIDAIKSYAKDRGWNIEILKDVGSGLSEKRKNYRKLLKMVINREVEKVIIAYPDRLTRFGFETLKEFFKSYGTEVIVINKKCKAPQEELVEDLITIISYFARKLYGMRSHKYKKLIKTVKEIVGENNAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBW6</accession>
    <name>F6BBW6_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Putative gamma-glutamylcyclotransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_1714</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97246.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799835.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF97246">
        <property type="protein sequence ID" value="AEF97246"/>
        <property type="gene ID" value="Metig_1714"/>
    </dbReference>
    <dbReference type="GeneID" id="10644588"/>
    <dbReference type="KEGG" id="mig:Metig_1714"/>
    <dbReference type="HOGENOM" id="CLU_2217094_0_0_2"/>
    <dbReference type="OMA" id="EIFDDYE"/>
    <dbReference type="OrthoDB" id="103617at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd06661">
        <property type="entry name" value="GGCT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045038">
        <property type="entry name" value="AIG2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009288">
        <property type="entry name" value="AIG2-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013024">
        <property type="entry name" value="GGCT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036568">
        <property type="entry name" value="GGCT-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31544">
        <property type="entry name" value="PTHR31544"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06094">
        <property type="entry name" value="GGACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110857">
        <property type="entry name" value="SSF110857"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GGACT" evidence="2">
        <location>
            <begin position="9"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00030602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF06094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF97246.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="112" mass="13511" checksum="6C5C9431DB506AA8" modified="2011-07-27" version="1">MHKNKKFNVFAYGELMKEERLKELINRVPKMKKGKIFGFEKFFDEGIGYYGIKKKEGSVVEGVILMDITEEELRIFDDYEDEGIYYFREKTKAYDEEGNEYDVYVYLRVKEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBX8</accession>
    <name>F6BBX8_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ribosomal RNA large subunit methyltransferase E</fullName>
            <ecNumber evidence="2">2.1.1.166</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">23S rRNA Um2552 methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="2">rRNA (uridine-2'-O-)-methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2" type="primary">rlmE</name>
        <name evidence="5" type="ordered locus">Metig_1726</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Specifically methylates the uridine in position 2552 of 23S rRNA at the 2'-O position of the ribose in the fully assembled 50S ribosomal subunit.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>S-adenosyl-L-methionine + uridine(2552) in 23S rRNA = 2'-O-methyluridine(2552) in 23S rRNA + H(+) + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:42720"/>
            <dbReference type="Rhea" id="RHEA-COMP:10202"/>
            <dbReference type="Rhea" id="RHEA-COMP:10203"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74478"/>
            <dbReference type="EC" id="2.1.1.166"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class I-like SAM-binding methyltransferase superfamily. RNA methyltransferase RlmE family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.166" evidence="2"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97258.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799847.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1726"/>
    <dbReference type="EnsemblBacteria" id="AEF97258">
        <property type="protein sequence ID" value="AEF97258"/>
        <property type="gene ID" value="Metig_1726"/>
    </dbReference>
    <dbReference type="GeneID" id="10644600"/>
    <dbReference type="KEGG" id="mig:Metig_1726"/>
    <dbReference type="HOGENOM" id="CLU_009422_4_4_2"/>
    <dbReference type="OMA" id="HRQTDHL"/>
    <dbReference type="OrthoDB" id="105478at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008650">
        <property type="term" value="F:rRNA (uridine-2'-O-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01547">
        <property type="entry name" value="RNA_methyltr_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002877">
        <property type="entry name" value="RNA_MeTrfase_FtsJ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015507">
        <property type="entry name" value="rRNA-MeTfrase_E"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01728">
        <property type="entry name" value="FtsJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005461">
        <property type="entry name" value="23S_rRNA_mtase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1 2 5" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="2 3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="FtsJ" evidence="4">
        <location>
            <begin position="26"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="2 3">
        <location>
            <position position="161"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="58"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="60"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="78"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="96"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="121"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR005461-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEF97258.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="245" mass="28462" checksum="B6A59E97F2C1C278" modified="2011-07-27" version="1">MGRKDKRWILQRKKDFYYNLAKKQKYRSRATFKLFQLNEKFRFMKEGDIVVDLGCAPGGWLQAAREIVGDRGFVVGVDLQSVKPLPYDNVKTIKGDMTKEETIQKIREILYPAKPTVVISDASPNISGVWDVDHARSIELTTIALKIATQLLKERGNFVVKVFQGDMFMDYVSLVEKYFEKVYPTKPRASRKESSEVYVVAKRYTGKPWEEEEPIKKEEKIEEQIGTEELLAKKIKEMRKLKANK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBZ0</accession>
    <name>F6BBZ0_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_0515</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96071.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798679.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96071">
        <property type="protein sequence ID" value="AEF96071"/>
        <property type="gene ID" value="Metig_0515"/>
    </dbReference>
    <dbReference type="GeneID" id="10643352"/>
    <dbReference type="KEGG" id="mig:Metig_0515"/>
    <dbReference type="HOGENOM" id="CLU_1329475_0_0_2"/>
    <dbReference type="OrthoDB" id="144568at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF96071.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="206" mass="24132" checksum="0759F3444F41D5D9" modified="2011-07-27" version="1">MEEVVAPNYSLPEKLRQHGGLDDKVLKTYAERVRSMEKKERFYKYISNNGTVIYHRGNKWRGLGEDVRISKEEYVKYRIKQVEDVKYAVNLTEKEKEQIINDILDELEELGVDTKEILKNLLKQEMREELEKGNRIAPIFWLSMDEQGNPCGEGIYKPLTLKDIQNCIKELREEGIAKAHEPVSIQKINIIKDTSNAIFLSIFWDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-06-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBZ1</accession>
    <name>F6BBZ1_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_0516</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96072.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798680.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96072">
        <property type="protein sequence ID" value="AEF96072"/>
        <property type="gene ID" value="Metig_0516"/>
    </dbReference>
    <dbReference type="GeneID" id="10643353"/>
    <dbReference type="KEGG" id="mig:Metig_0516"/>
    <dbReference type="HOGENOM" id="CLU_2911526_0_0_2"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF96072.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="61" mass="7194" checksum="C801741CB7B4B922" modified="2011-07-27" version="1">MKEYKIGDKSVWIAYDGHSKKYKIEVRKGVEVVGEMECSKKQGERIIEFLELLGVEKIWDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>F6BC04</accession>
    <name>F6BC04_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcriptional regulator, TrmB</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0530</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96085.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798693.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0530"/>
    <dbReference type="EnsemblBacteria" id="AEF96085">
        <property type="protein sequence ID" value="AEF96085"/>
        <property type="gene ID" value="Metig_0530"/>
    </dbReference>
    <dbReference type="GeneID" id="10643368"/>
    <dbReference type="KEGG" id="mig:Metig_0530"/>
    <dbReference type="HOGENOM" id="CLU_140786_1_0_2"/>
    <dbReference type="OMA" id="FTLETIM"/>
    <dbReference type="OrthoDB" id="96961at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002831">
        <property type="entry name" value="Tscrpt_reg_TrmB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01978">
        <property type="entry name" value="TrmB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TrmB" evidence="1">
        <location>
            <begin position="24"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96085.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="130" mass="15180" checksum="D12F7B683072DA5B" modified="2011-07-27" version="1">MGNKLILKTPCTTFTLENLMCCTFGLKVFDVVVYFEILKNGPSRINKIAEKLNRDRSTVQRAVQNLMNVGLVKRKQVNIKEGGYFYIYEALPFEEVKRIIKETVTQWCESVKQWIDEIEVEDVINEELKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>F6BC48</accession>
    <name>F6BC48_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Phenylalanine--tRNA ligase beta subunit</fullName>
            <ecNumber evidence="10">6.1.1.20</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Phenylalanyl-tRNA synthetase beta subunit</fullName>
            <shortName evidence="10">PheRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">pheT</name>
        <name evidence="12" type="ordered locus">Metig_0575</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + H(+) + L-phenylalanyl-tRNA(Phe)</text>
            <dbReference type="Rhea" id="RHEA:19413"/>
            <dbReference type="Rhea" id="RHEA-COMP:9668"/>
            <dbReference type="Rhea" id="RHEA-COMP:9699"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58095"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78531"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.20"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="4 10">Tetramer of two alpha and two beta subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 10">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the phenylalanyl-tRNA synthetase beta subunit family. Type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.20" evidence="10"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96129.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798736.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0575"/>
    <dbReference type="EnsemblBacteria" id="AEF96129">
        <property type="protein sequence ID" value="AEF96129"/>
        <property type="gene ID" value="Metig_0575"/>
    </dbReference>
    <dbReference type="GeneID" id="10643413"/>
    <dbReference type="KEGG" id="mig:Metig_0575"/>
    <dbReference type="HOGENOM" id="CLU_020279_3_0_2"/>
    <dbReference type="OMA" id="FPGRCAN"/>
    <dbReference type="OrthoDB" id="55024at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004826">
        <property type="term" value="F:phenylalanine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006432">
        <property type="term" value="P:phenylalanyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00769">
        <property type="entry name" value="PheRS_beta_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00284">
        <property type="entry name" value="Phe_tRNA_synth_beta2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005146">
        <property type="entry name" value="B3/B4_tRNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009061">
        <property type="entry name" value="DNA-bd_dom_put_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045060">
        <property type="entry name" value="Phe-tRNA-ligase_IIc_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004531">
        <property type="entry name" value="Phe-tRNA-synth_IIc_bsu_arc_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020825">
        <property type="entry name" value="Phe-tRNA_synthase-like_B3/B4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022918">
        <property type="entry name" value="Phe_tRNA_ligase_beta2_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041616">
        <property type="entry name" value="PheRS_beta_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040659">
        <property type="entry name" value="PhetRS_B1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005147">
        <property type="entry name" value="tRNA_synthase_B5-dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10947">
        <property type="entry name" value="PTHR10947"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03483">
        <property type="entry name" value="B3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03484">
        <property type="entry name" value="B5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18262">
        <property type="entry name" value="PhetRS_B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17759">
        <property type="entry name" value="tRNA_synthFbeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00873">
        <property type="entry name" value="B3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00874">
        <property type="entry name" value="B5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46955">
        <property type="entry name" value="SSF46955"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="SSF55681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00471">
        <property type="entry name" value="pheT_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51483">
        <property type="entry name" value="B5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10 12" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 10 12" id="KW-0436">Ligase</keyword>
    <keyword evidence="6 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8 10" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="B5" evidence="11">
        <location>
            <begin position="273"/>
            <end position="348"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AEF96129.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="548" mass="62681" checksum="380EB751CAE36D5E" modified="2011-07-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>F6BC60</accession>
    <name>F6BC60_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ADP-specific phosphofructokinase</fullName>
            <ecNumber evidence="3">2.7.1.146</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">ADP-dependent phosphofructokinase</fullName>
            <shortName evidence="3">ADP-Pfk</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">pfkC</name>
        <name evidence="4" type="ordered locus">Metig_1734</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the phosphorylation of fructose 6-phosphate to fructose 1,6-bisphosphate using ADP as the phosphate donor.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>ADP + beta-D-fructose 6-phosphate = AMP + beta-D-fructose 1,6-bisphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:20105"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <dbReference type="ChEBI" id="CHEBI:57634"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.146"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="3">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Carbohydrate degradation; glycolysis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the carbohydrate kinase PfkC family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.146" evidence="3"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97266.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799855.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1734"/>
    <dbReference type="EnsemblBacteria" id="AEF97266">
        <property type="protein sequence ID" value="AEF97266"/>
        <property type="gene ID" value="Metig_1734"/>
    </dbReference>
    <dbReference type="GeneID" id="10644608"/>
    <dbReference type="KEGG" id="mig:Metig_1734"/>
    <dbReference type="HOGENOM" id="CLU_046643_0_0_2"/>
    <dbReference type="OMA" id="NRIFEFR"/>
    <dbReference type="OrthoDB" id="15153at2157"/>
    <dbReference type="UniPathway" id="UPA00109"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043844">
        <property type="term" value="F:ADP-specific phosphofructokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008443">
        <property type="term" value="F:phosphofructokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006000">
        <property type="term" value="P:fructose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00561">
        <property type="entry name" value="ADP_PFKinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007666">
        <property type="entry name" value="ADP_PFK/GK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015990">
        <property type="entry name" value="ADP_PFK/GK_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011790">
        <property type="entry name" value="ADP_PFK_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21208">
        <property type="entry name" value="PTHR21208"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04587">
        <property type="entry name" value="ADP_PFK_GK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015883">
        <property type="entry name" value="ADP-Pfk_glckin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="SSF53613"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02045">
        <property type="entry name" value="P_fruct_ADP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51255">
        <property type="entry name" value="ADPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 3" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="3 4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0808">Transferase</keyword>
    <feature type="active site" description="Proton acceptor" evidence="3">
        <location>
            <position position="443"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="270"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="300"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="443"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEF97266.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="464" mass="53111" checksum="07D610A77EEC1E7F" modified="2011-07-27" version="1">MEIKEYLNNLSNLSIFLAYNANVDTIKYLNDKDVQELIDLFDVNEIIKKIEEYPRIIKSPLDFVARLIHSMKTGKPAEVPLYDDEEINKWFNRFKYDEERMGGQVGIISNLLSILGLKKVIAYTPILSKKQAEMFVNKDNLLYPSVVDGNLILKKPIESYREDDPLKINRIFEYKKGVKFRLGDEEITTPQANRFIVASRPENLRIEIRDELREKLPKIGDMVDCAILSGYQAIKEEYKDGKTGDYYLKKAKEDIKLLKKNNKNLKVHLEFASIPNRAIRKKIADNLLPNVDCVGMDETELANVLNVIGYDKLSNEILKKSWVEDVVEGSKILLEEYDLEIVQVHTMYYIMYLCREDNPLTNEELKKTLEFATILASTKAALGNIGDVDDLKVGLEVPYNEYRDLLASIVAELSKKPENKDYRMLLVPSRLVKNPKSTVGLGDTISSGAFVGYVSFLKNKLNSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCA0</accession>
    <name>F6BCA0_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_1774</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97306.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799895.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1774"/>
    <dbReference type="EnsemblBacteria" id="AEF97306">
        <property type="protein sequence ID" value="AEF97306"/>
        <property type="gene ID" value="Metig_1774"/>
    </dbReference>
    <dbReference type="GeneID" id="10644648"/>
    <dbReference type="KEGG" id="mig:Metig_1774"/>
    <dbReference type="HOGENOM" id="CLU_507744_0_0_2"/>
    <dbReference type="OMA" id="FEDYEPV"/>
    <dbReference type="OrthoDB" id="7749at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1360">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002846">
        <property type="entry name" value="NRD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038982">
        <property type="entry name" value="NrpR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036984">
        <property type="entry name" value="NrpR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013668">
        <property type="entry name" value="RNase_R_HTH_12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41964">
        <property type="entry name" value="PTHR41964"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08461">
        <property type="entry name" value="HTH_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01995">
        <property type="entry name" value="NRD1_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH_12" evidence="2">
        <location>
            <begin position="10"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="domain" description="NRD1_2" evidence="1">
        <location>
            <begin position="85"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="domain" description="NRD1_2" evidence="1">
        <location>
            <begin position="320"/>
            <end position="534"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF97306.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="540" mass="60592" checksum="2FE42BCD3F57CCC2" modified="2011-07-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCB7</accession>
    <name>F6BCB7_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 4">3,4-dihydroxy-2-butanone 4-phosphate synthase</fullName>
            <shortName evidence="3 4">DHBP synthase</shortName>
            <ecNumber evidence="3 4">4.1.99.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">ribB</name>
        <name evidence="5" type="ordered locus">Metig_1791</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3 4">Catalyzes the conversion of D-ribulose 5-phosphate to formate and 3,4-dihydroxy-2-butanone 4-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3 4">
            <text>D-ribulose 5-phosphate = (2S)-2-hydroxy-3-oxobutyl phosphate + formate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18457"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:58121"/>
            <dbReference type="ChEBI" id="CHEBI:58830"/>
            <dbReference type="EC" id="4.1.99.12"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3 4">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="3 4">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="3 4">Binds 2 divalent metal cations per subunit. Magnesium or manganese.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3 4">Cofactor biosynthesis; riboflavin biosynthesis; 2-hydroxy-3-oxobutyl phosphate from D-ribulose 5-phosphate: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 4">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 4">Belongs to the DHBP synthase family.</text>
    </comment>
    <dbReference type="EC" id="4.1.99.12" evidence="3 4"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97323.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799912.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1791"/>
    <dbReference type="EnsemblBacteria" id="AEF97323">
        <property type="protein sequence ID" value="AEF97323"/>
        <property type="gene ID" value="Metig_1791"/>
    </dbReference>
    <dbReference type="GeneID" id="10644668"/>
    <dbReference type="KEGG" id="mig:Metig_1791"/>
    <dbReference type="HOGENOM" id="CLU_020273_3_2_2"/>
    <dbReference type="OMA" id="DAGGLIC"/>
    <dbReference type="OrthoDB" id="72536at2157"/>
    <dbReference type="UniPathway" id="UPA00275">
        <property type="reaction ID" value="UER00399"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008686">
        <property type="term" value="F:3,4-dihydroxy-2-butanone-4-phosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009231">
        <property type="term" value="P:riboflavin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00180">
        <property type="entry name" value="RibB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017945">
        <property type="entry name" value="DHBP_synth_RibB-like_a/b_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000422">
        <property type="entry name" value="DHBP_synthase_RibB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00926">
        <property type="entry name" value="DHBP_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55821">
        <property type="entry name" value="SSF55821"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00506">
        <property type="entry name" value="ribB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0456">Lyase</keyword>
    <keyword evidence="3 4" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3 4" id="KW-0464">Manganese</keyword>
    <keyword evidence="2 3 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3 4" id="KW-0686">Riboflavin biosynthesis</keyword>
    <feature type="region of interest" description="D-ribulose 5-phosphate binding" evidence="3">
        <location>
            <begin position="25"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="D-ribulose 5-phosphate binding" evidence="3">
        <location>
            <begin position="161"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="26"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="30"/>
        </location>
        <ligand>
            <name>D-ribulose 5-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58121"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="164"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="site" description="Essential for catalytic activity" evidence="3">
        <location>
            <position position="147"/>
        </location>
    </feature>
    <feature type="site" description="Essential for catalytic activity" evidence="3">
        <location>
            <position position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEF97323.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="228" mass="25808" checksum="FFAFBA684EA2A867" modified="2011-07-27" version="1">MNIVEKAMQALKNGKIVLVYDADDREGETDMVVASEKITSEHIRIMRKDGGGLICTAIHPEFCKKLGIPFMVDILDFASQKFKVLKELYPNDIPYDEKSSFSITVNHRKTFTGITDNDRALTIKSLAQLCKEKRFDDFGKEFRSPGHVTLLRAADGLVKKRQGHTEMTVALAEMAGLTPITTICEMMGDDGNAMSKSETKKYAEKYNLVYLNGEELINYYLDYIEKKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCE7</accession>
    <name>F6BCE7_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Dimethylmenaquinone methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_0604</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96158.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798764.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0604"/>
    <dbReference type="EnsemblBacteria" id="AEF96158">
        <property type="protein sequence ID" value="AEF96158"/>
        <property type="gene ID" value="Metig_0604"/>
    </dbReference>
    <dbReference type="GeneID" id="10643442"/>
    <dbReference type="KEGG" id="mig:Metig_0604"/>
    <dbReference type="HOGENOM" id="CLU_072626_3_1_2"/>
    <dbReference type="OMA" id="VNVPVVC"/>
    <dbReference type="OrthoDB" id="103576at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16841">
        <property type="entry name" value="RraA_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005493">
        <property type="entry name" value="RraA/RraA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036704">
        <property type="entry name" value="RraA/RraA-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03737">
        <property type="entry name" value="RraA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89562">
        <property type="entry name" value="SSF89562"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF96158.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="193" mass="20703" checksum="6B1FDB2BECB8F91E" modified="2011-07-27" version="1">MKILNTFSVPNLCDAGARVLKGIKPINPMQKIVVGEALTVKTSPHDWGTVVKAIKYARNKIIVVDANGGDIAVWGGLASTNAKLKGVLGIVIDGGIRDVEDINALKFPAFAKHIVPNAGKPLDEGEINVPITCGGELINPKDIIVGDCNGVAVIKREELSEIIENAKAIKEKETNIRNKILRGMDLGDILRLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCJ0</accession>
    <name>F6BCJ0_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 11">Aspartate-semialdehyde dehydrogenase</fullName>
            <shortName evidence="11">ASA dehydrogenase</shortName>
            <shortName evidence="11">ASADH</shortName>
            <ecNumber evidence="7 11">1.2.1.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Aspartate-beta-semialdehyde dehydrogenase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">asd</name>
        <name evidence="13" type="ordered locus">Metig_0651</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes the NADPH-dependent formation of L-aspartate-semialdehyde (L-ASA) by the reductive dephosphorylation of L-aspartyl-4-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 11">
            <text>L-aspartate 4-semialdehyde + NADP(+) + phosphate = 4-phospho-L-aspartate + H(+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:24284"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57535"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:537519"/>
            <dbReference type="EC" id="1.2.1.11"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 11">Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; (S)-tetrahydrodipicolinate from L-aspartate: step 2/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 11">Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-homoserine from L-aspartate: step 2/3.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4 11">Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 2/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6 11">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5 11">Belongs to the aspartate-semialdehyde dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.11" evidence="7 11"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96201.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798804.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0651"/>
    <dbReference type="EnsemblBacteria" id="AEF96201">
        <property type="protein sequence ID" value="AEF96201"/>
        <property type="gene ID" value="Metig_0651"/>
    </dbReference>
    <dbReference type="GeneID" id="10643491"/>
    <dbReference type="KEGG" id="mig:Metig_0651"/>
    <dbReference type="HOGENOM" id="CLU_049966_1_0_2"/>
    <dbReference type="OMA" id="CEEEMKM"/>
    <dbReference type="OrthoDB" id="26718at2157"/>
    <dbReference type="UniPathway" id="UPA00034">
        <property type="reaction ID" value="UER00016"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00050">
        <property type="reaction ID" value="UER00463"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00051">
        <property type="reaction ID" value="UER00464"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004073">
        <property type="term" value="F:aspartate-semialdehyde dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071266">
        <property type="term" value="P:'de novo' L-methionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019877">
        <property type="term" value="P:diaminopimelate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009097">
        <property type="term" value="P:isoleucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009089">
        <property type="term" value="P:lysine biosynthetic process via diaminopimelate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009088">
        <property type="term" value="P:threonine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02121">
        <property type="entry name" value="ASADH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005676">
        <property type="entry name" value="Asp_semi-ald_DH_pep-lack"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012080">
        <property type="entry name" value="Asp_semialdehyde_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000534">
        <property type="entry name" value="Semialdehyde_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012280">
        <property type="entry name" value="Semialdhyde_DH_dimer_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01118">
        <property type="entry name" value="Semialdhyde_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02774">
        <property type="entry name" value="Semialdhyde_dhC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00859">
        <property type="entry name" value="Semialdhyde_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00978">
        <property type="entry name" value="asd_EA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="11" id="KW-0220">Diaminopimelate biosynthesis</keyword>
    <keyword evidence="9 11" id="KW-0457">Lysine biosynthesis</keyword>
    <keyword evidence="10 11" id="KW-0486">Methionine biosynthesis</keyword>
    <keyword evidence="11" id="KW-0521">NADP</keyword>
    <keyword evidence="11 13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 11" id="KW-0791">Threonine biosynthesis</keyword>
    <feature type="domain" description="Semialdhyde_dh" evidence="12">
        <location>
            <begin position="3"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-thioester intermediate" evidence="11">
        <location>
            <position position="150"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="11">
        <location>
            <position position="243"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="10"/>
            <end position="13"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="37"/>
            <end position="38"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="111"/>
        </location>
        <ligand>
            <name>phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="177"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="180"/>
            <end position="181"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="203"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="206"/>
        </location>
        <ligand>
            <name>phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="236"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="326"/>
            <end position="327"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00013120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AEF96201.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="347" mass="38534" checksum="B3A8CEE2FD865D2A" modified="2011-07-27" version="1">MIKVGVLGATGMVGQRFIQMLADHPMFELEVLAASPRSAGKKYKDAAYWYQAEPIPEEIGEMVVVPTDPRHEAFKDVDIVFSALPADLAREIEPAFAKEGKLVFSNASAMRMEKDVPLIVPEVNEEHFKLIEIQRENRKWDGAIVTNPNCSTICLVITLKPILDKFGIEAVNVTTLQAVSGAGYDGVPSMAIIDNVIPYIKNEEEKMQTESLKILGEFDGSEVKFADFKVGASCNRICVIDGHVESIFVKTKEPAEPEDIKEVMDKFDPLKDLDLPTYAKPIVVKEEPDRPQPRLDRNEGNGMSIVVGRIRKDPIFTVKYTALEHNTIRGAAGASVLNAELFVKKYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCJ1</accession>
    <name>F6BCJ1_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_0652</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96202.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798805.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0652"/>
    <dbReference type="EnsemblBacteria" id="AEF96202">
        <property type="protein sequence ID" value="AEF96202"/>
        <property type="gene ID" value="Metig_0652"/>
    </dbReference>
    <dbReference type="GeneID" id="10643492"/>
    <dbReference type="KEGG" id="mig:Metig_0652"/>
    <dbReference type="HOGENOM" id="CLU_076814_1_0_2"/>
    <dbReference type="OMA" id="IGKKLMW"/>
    <dbReference type="OrthoDB" id="112466at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007003">
        <property type="entry name" value="DUF655"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40734">
        <property type="entry name" value="PTHR40734"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04919">
        <property type="entry name" value="DUF655"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF96202.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="209" mass="25340" checksum="5B926005091AAD90" modified="2011-07-27" version="1">MVKKYFKAKKRKDFEDYAWVLDYLPYGHPDDPRPIHERKPIAQAIGEKQFVLMELVLKDDTEVELAERVYIGRGKRDKVDYVARMIKYDDLTNTAKTELLYVVMEAVKNNEERFINFLNKCQSITPKKHMLELLPEIGKKHMWQIIEEREKKPFESFKDFEERTHKDIVRVIAKRIVKELEEDQKYYLFVKWKRGLILNEEKMTFYLKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCP8</accession>
    <name>F6BCP8_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Molybdopterin biosynthesis MoaE protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_0709</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96259.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798862.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0709"/>
    <dbReference type="EnsemblBacteria" id="AEF96259">
        <property type="protein sequence ID" value="AEF96259"/>
        <property type="gene ID" value="Metig_0709"/>
    </dbReference>
    <dbReference type="GeneID" id="10643549"/>
    <dbReference type="KEGG" id="mig:Metig_0709"/>
    <dbReference type="HOGENOM" id="CLU_089568_1_2_2"/>
    <dbReference type="OMA" id="WPLQRVS"/>
    <dbReference type="OrthoDB" id="62565at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006777">
        <property type="term" value="P:Mo-molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00756">
        <property type="entry name" value="MoaE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1170.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036563">
        <property type="entry name" value="MoaE_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003448">
        <property type="entry name" value="Mopterin_biosynth_MoaE"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02391">
        <property type="entry name" value="MoaE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54690">
        <property type="entry name" value="SSF54690"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF96259.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="146" mass="16881" checksum="83BE7091F60F3BE5" modified="2011-07-27" version="1">MIKITKEDFNVEEEIKKMLEKHKNIGGVVTFVGVVRNVGIKDGEEKEVEKLEFECYEKMAIKKLEEIREEAMKKYDIIDAIIIHRIGTLKVGEKIVLIAVGAKHRKDAFLACEYLIDELKKVVPIWKKEYAKDGSYWVEYSGSTTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCR2</accession>
    <name>F6BCR2_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">5'-nucleotidase SurE</fullName>
            <ecNumber evidence="4">3.1.3.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Nucleoside 5'-monophosphate phosphohydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">surE</name>
        <name evidence="6" type="ordered locus">Metig_0723</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Nucleotidase that shows phosphatase activity on nucleoside 5'-monophosphates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>a ribonucleoside 5'-phosphate + H2O = a ribonucleoside + phosphate</text>
            <dbReference type="Rhea" id="RHEA:12484"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:18254"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58043"/>
            <dbReference type="EC" id="3.1.3.5"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
        <text evidence="4">Binds 1 divalent metal cation per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the SurE nucleotidase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.5" evidence="4"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96273.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0723"/>
    <dbReference type="EnsemblBacteria" id="AEF96273">
        <property type="protein sequence ID" value="AEF96273"/>
        <property type="gene ID" value="Metig_0723"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0723"/>
    <dbReference type="HOGENOM" id="CLU_045192_1_3_2"/>
    <dbReference type="OMA" id="DCVHIAL"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106411">
        <property type="term" value="F:XMP 5'-nucleosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1210.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00060">
        <property type="entry name" value="SurE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030048">
        <property type="entry name" value="SurE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002828">
        <property type="entry name" value="SurE-like_Pase/nucleotidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036523">
        <property type="entry name" value="SurE-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30457">
        <property type="entry name" value="PTHR30457"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01975">
        <property type="entry name" value="SurE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64167">
        <property type="entry name" value="SSF64167"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00087">
        <property type="entry name" value="surE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SurE" evidence="5">
        <location>
            <begin position="4"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="9"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="10"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="42"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="98"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEF96273.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="265" mass="29384" checksum="3DCE4560974DA401" modified="2011-07-27" version="1">MLDILLVNDDGIYSNGLIALKDALLEKFNARITIVAPTNQQSGIGRAISLFEPLRITKTKLADGSWGYAVSGTPTDCVILGIYEILKKVPDVVISGINIGENLGTEITTSGTLGAAFEGAHHGAKALASSLQVTSDHLKFKEGETPIDFTVPARITANVVEKMLDYDFPCDVVNLNIPEGATEKTPIEITRLARKMYTTHVEERIDPRGRSYYWIDGYPILEEEEDTDVYVVRRKGHISLTPLTLDTTIKNLEEFKKKYERILNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCU6</accession>
    <name>F6BCU6_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Carbonic anhydrase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0761</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the beta-class carbonic anhydrase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96307.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0761"/>
    <dbReference type="EnsemblBacteria" id="AEF96307">
        <property type="protein sequence ID" value="AEF96307"/>
        <property type="gene ID" value="Metig_0761"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0761"/>
    <dbReference type="HOGENOM" id="CLU_084253_3_0_2"/>
    <dbReference type="OMA" id="AIFTCMD"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004089">
        <property type="term" value="F:carbonate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001765">
        <property type="entry name" value="Carbonic_anhydrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036874">
        <property type="entry name" value="Carbonic_anhydrase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43175">
        <property type="entry name" value="PTHR43175"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00484">
        <property type="entry name" value="Pro_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00947">
        <property type="entry name" value="Pro_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53056">
        <property type="entry name" value="SSF53056"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96307.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="159" mass="17507" checksum="90DC7F8C2F8662F0" modified="2011-07-27" version="1">MILNTNGGINLGNVKPKKKLAIVTCMDARLVNFLSEKLGVKRGDAKVIKNAGNIITDDVIRSLVVAIYCLGVEKVMVVGHTDCGMKSIDVEEIKKKMIERGANPYFTPNLKCWLGKIDDEEKNVIEGVNIIKNHPAIPKDITVEGYLIDVETGELKKLC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-06-02" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCW0</accession>
    <name>F6BCW0_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_0775</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96321.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798924.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96321">
        <property type="protein sequence ID" value="AEF96321"/>
        <property type="gene ID" value="Metig_0775"/>
    </dbReference>
    <dbReference type="GeneID" id="10643615"/>
    <dbReference type="KEGG" id="mig:Metig_0775"/>
    <dbReference type="HOGENOM" id="CLU_1607166_0_0_2"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF96321.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="165" mass="19707" checksum="57161DED7AB6C49F" modified="2011-07-27" version="1">MRINNNINLNDFKTNNFSKEHESLKNLPKEFINFDEIGKGCWSQLRTVDMLDVKDNSFILMEITNLKNTLYNKSKKFKISVEIAKEFIKSEYREKLMESLLLLSLLTNNLNNKRITFKLILYDKKEDIFAYEYLKSYLNSILQEGDKCKITHIEDFIKYLNKQIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCY8</accession>
    <name>F6BCY8_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cobyrinate a,c-diamide synthase</fullName>
            <ecNumber evidence="3">6.3.5.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Cobyrinic acid a,c-diamide synthetase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="3">Ni-sirohydrochlorin a,c-diamide synthase</fullName>
            <ecNumber evidence="3">6.3.5.12</ecNumber>
        </alternativeName>
        <alternativeName>
            <fullName evidence="3">Ni-sirohydrochlorin a,c-diamide synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">cbiA</name>
        <name evidence="3" type="synonym">cfbB</name>
        <name evidence="6" type="ordered locus">Metig_0804</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the ATP-dependent amidation of the two carboxylate groups at positions a and c of cobyrinate, using either L-glutamine or ammonia as the nitrogen source. Involved in the biosynthesis of the unique nickel-containing tetrapyrrole coenzyme F430, the prosthetic group of methyl-coenzyme M reductase (MCR), which plays a key role in methanogenesis and anaerobic methane oxidation. Catalyzes the ATP-dependent amidation of the two carboxylate groups at positions a and c of Ni-sirohydrochlorin, using L-glutamine or ammonia as the nitrogen source.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>2 ATP + 2 H2O + 2 L-glutamine + Ni-sirohydrochlorin = 2 ADP + 2 H(+) + 2 L-glutamate + Ni-sirohydrochlorin a,c-diamide + 2 phosphate</text>
            <dbReference type="Rhea" id="RHEA:52896"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:136841"/>
            <dbReference type="ChEBI" id="CHEBI:136887"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.5.12"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>2 ATP + cob(II)yrinate + 2 H2O + 2 L-glutamine = 2 ADP + cob(II)yrinate a,c diamide + 2 H(+) + 2 L-glutamate + 2 phosphate</text>
            <dbReference type="Rhea" id="RHEA:26289"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:58537"/>
            <dbReference type="ChEBI" id="CHEBI:58894"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.5.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 3">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Cofactor biosynthesis; adenosylcobalamin biosynthesis; cob(II)yrinate a,c-diamide from sirohydrochlorin (anaerobic route): step 10/10.</text>
    </comment>
    <comment type="domain">
        <text evidence="3">Comprises of two domains. The C-terminal domain contains the binding site for glutamine and catalyzes the hydrolysis of this substrate to glutamate and ammonia. The N-terminal domain is anticipated to bind ATP, and cobyrinate or Ni-sirohydrochlorin, and catalyzes the ultimate synthesis of the diamide product. The ammonia produced via the glutaminase domain is probably translocated to the adjacent domain via a molecular tunnel, where it reacts with an activated intermediate.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="3">The a and c carboxylates of cobyrinate and Ni-sirohydrochlorin are activated for nucleophilic attack via formation of a phosphorylated intermediate by ATP. CbiA catalyzes first the amidation of the c-carboxylate, and then that of the a-carboxylate.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CobB/CbiA family.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.11" evidence="3"/>
    <dbReference type="EC" id="6.3.5.12" evidence="3"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96349.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798951.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0804"/>
    <dbReference type="EnsemblBacteria" id="AEF96349">
        <property type="protein sequence ID" value="AEF96349"/>
        <property type="gene ID" value="Metig_0804"/>
    </dbReference>
    <dbReference type="GeneID" id="10643645"/>
    <dbReference type="KEGG" id="mig:Metig_0804"/>
    <dbReference type="HOGENOM" id="CLU_022752_2_0_2"/>
    <dbReference type="OMA" id="MYLTNSI"/>
    <dbReference type="OrthoDB" id="14023at2157"/>
    <dbReference type="UniPathway" id="UPA00148">
        <property type="reaction ID" value="UER00231"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042242">
        <property type="term" value="F:cobyrinic acid a,c-diamide synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006541">
        <property type="term" value="P:glutamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015948">
        <property type="term" value="P:methanogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00027">
        <property type="entry name" value="CobB_CbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004484">
        <property type="entry name" value="CbiA_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002586">
        <property type="entry name" value="CobQ/CobB/MinD/ParA_Nub-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011698">
        <property type="entry name" value="GATase_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43873">
        <property type="entry name" value="PTHR43873"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01656">
        <property type="entry name" value="CbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07685">
        <property type="entry name" value="GATase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="SSF52317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00379">
        <property type="entry name" value="cobB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword evidence="2 3" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-0484">Methanogenesis</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CbiA" evidence="4">
        <location>
            <begin position="4"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="domain" description="GATase_3" evidence="5">
        <location>
            <begin position="247"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="3">
        <location>
            <position position="326"/>
        </location>
    </feature>
    <feature type="site" description="Increases nucleophilicity of active site Cys" evidence="3">
        <location>
            <position position="424"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF07685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEF96349.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="443" mass="49579" checksum="5872E047F493D546" modified="2011-07-27" version="1">MRRVVIAGTSSMVGKTTISTGIMKALSKKYNVQPYKVGPDYIDPTYHTIATNNKSRNLDSFFMSGEQIKALFKKHSKDKDISVIEGVRGLYEGISPYNDIGSTASVAKALNAPVILLMNARSLTRSAAAIIKGFRAFDSQVNIKGVIFNMVRGEKHIKKLEEAVKYYIPDIEIIGAIPRDESLNVPQRHLGLIPTPENKKALEEKINLWGEIVEKYLDLDKLVEISDVDDFEVNGGELWEINKNKCKIGVAYDEVFNFYYWDNFDALEENGAKIEFFSPLNDDDIPNCDIIYIGGGYPEEFAEKLSSNKDMLDAIRNFDGKIYGECGGLMYLTNSINGVEMLGLLDCDAVMTKNVQGLSYVEGEFVENCVIGKKGLKFRAHEFHYSKLINIREKIFAYKINRGRGIINSMDGIKKGNVLGGYAHQHCVANPYFASTMVNSVDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-06-02" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>F6BD03</accession>
    <name>F6BD03_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">HNH endonuclease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0819</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96364.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798966.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96364">
        <property type="protein sequence ID" value="AEF96364"/>
        <property type="gene ID" value="Metig_0819"/>
    </dbReference>
    <dbReference type="GeneID" id="10643660"/>
    <dbReference type="KEGG" id="mig:Metig_0819"/>
    <dbReference type="HOGENOM" id="CLU_851571_0_0_2"/>
    <dbReference type="OMA" id="CKQCHYR"/>
    <dbReference type="OrthoDB" id="95121at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00085">
        <property type="entry name" value="HNHc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002711">
        <property type="entry name" value="HNH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003615">
        <property type="entry name" value="HNH_nuc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036280">
        <property type="entry name" value="Multihaem_cyt_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01844">
        <property type="entry name" value="HNH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00507">
        <property type="entry name" value="HNHc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48695">
        <property type="entry name" value="SSF48695"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0540">Nuclease</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HNHc" evidence="1">
        <location>
            <begin position="12"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="domain" description="HNHc" evidence="1">
        <location>
            <begin position="138"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96364.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="276" mass="32581" checksum="338E1B7D562BAA78" modified="2011-07-27" version="1">MGNVKNKGLSSRIRKKILERDEYKCNICGHKYLQIHILKNGNLSILCKQCHYRAHISDKINEYSNFLFTVVQKILESKLVITINSKSENILIYNKDLIKDVVLRKLKYVLSHKITKIIMEEIDKDIETELRVVSEDIKEQLILKNRHNCDKCGYSYLEIHHIDKNSENNNFENLITLCRECHRNIHYDLYQKTGVGNVEKGFNFLFSRIYEYIDSHLNFEDSVIVDVKSSNNESIEIEFDNIENLNQIFGNKNIDDIFNELNKKISNYIQYLIPIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-06-02" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>F6BD24</accession>
    <name>F6BD24_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0840</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96385.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798987.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96385">
        <property type="protein sequence ID" value="AEF96385"/>
        <property type="gene ID" value="Metig_0840"/>
    </dbReference>
    <dbReference type="GeneID" id="10643681"/>
    <dbReference type="KEGG" id="mig:Metig_0840"/>
    <dbReference type="HOGENOM" id="CLU_123707_0_0_2"/>
    <dbReference type="OMA" id="KKTCMEL"/>
    <dbReference type="OrthoDB" id="107493at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="31"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96385.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="177" mass="20896" checksum="4092B28AA792546F" modified="2011-07-27" version="1">MLSFEDYPSIEEIKKEKEILHQEGIKKIEYLKKIRADLDKLRSQYDDEILNNQLSIKIKKLEDELANTIANLAKIDTALKILDATEYILENNIFGDKWGIITSKIKFEELMDIIVDENFDLARIYKKLYDLAGINDKKILKKIENLNQELLDEPKEEHLEKNDSIVDRTKNIKKFLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>F6BD28</accession>
    <name>F6BD28_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Type 2 DNA topoisomerase 6 subunit A</fullName>
            <ecNumber evidence="7">5.6.2.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Type II DNA topoisomerase VI subunit A</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">top6A</name>
        <name evidence="9" type="ordered locus">Metig_0845</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Relaxes both positive and negative superturns and exhibits a strong decatenase activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>ATP-dependent breakage, passage and rejoining of double-stranded DNA.</text>
            <dbReference type="EC" id="5.6.2.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 7">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homodimer. Heterotetramer of two Top6A and two Top6B chains.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the TOP6A family.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.2" evidence="7"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96389.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798991.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0845"/>
    <dbReference type="EnsemblBacteria" id="AEF96389">
        <property type="protein sequence ID" value="AEF96389"/>
        <property type="gene ID" value="Metig_0845"/>
    </dbReference>
    <dbReference type="GeneID" id="10643686"/>
    <dbReference type="KEGG" id="mig:Metig_0845"/>
    <dbReference type="HOGENOM" id="CLU_037229_1_0_2"/>
    <dbReference type="OMA" id="NWGEARF"/>
    <dbReference type="OrthoDB" id="22842at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003918">
        <property type="term" value="F:DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006265">
        <property type="term" value="P:DNA topological change"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006268">
        <property type="term" value="P:DNA unwinding involved in DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00223">
        <property type="entry name" value="TOPRIM_TopoIIB_SPO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00132">
        <property type="entry name" value="Top6A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002815">
        <property type="entry name" value="Spo11/TopoVI_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013049">
        <property type="entry name" value="Spo11/TopoVI_A_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036078">
        <property type="entry name" value="Spo11/TopoVI_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004085">
        <property type="entry name" value="TopoVI_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034136">
        <property type="entry name" value="TOPRIM_Topo6A/Spo11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10848">
        <property type="entry name" value="PTHR10848"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04406">
        <property type="entry name" value="TP6A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01550">
        <property type="entry name" value="TOP6AFAMILY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01552">
        <property type="entry name" value="TPISMRASE6A"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56726">
        <property type="entry name" value="SSF56726"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 7 9" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4 7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0799">Topoisomerase</keyword>
    <feature type="domain" description="TP6A_N" evidence="8">
        <location>
            <begin position="71"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="7">
        <location>
            <position position="98"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="193"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="245"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AEF96389.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="365" mass="41660" checksum="366F95A5B802CB3B" modified="2011-07-27" version="1">MVISKSREIARKKILELANKMYEDLLKGKRPKIKLPIRSLTNARFDSETGTFMLMGKEKERTLTVNQAKIFAQTVKMLEFSKQLLDSNDFSTLREAYYVSKNWGEARFDDQQPSNNVIEDLEAALDVLREDLGFIPEEDGSAVVGPLKIIDRTPEGEEIKVDCTKLGTGAYNIPNDVTRLEFETNADFILAIETAGMFARLNAEKFWDKHNCILVSLKGVPARATRRFIKRLNEEHDIPVLVFTDGDPYGYLNIYRTLKVGSGKAVHLADKLAVPSARLIGVTPQDIIDYDLPTHPLKEQDIKRIKDGLKNDDFVKSFPEWQKALKQMLDMKVRAEQQSLAKYGLKYVVDTYLPEKIKDEKTWLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>F6BD40</accession>
    <name>F6BD40_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Hydrogenase expression/formation protein HypE</fullName>
            <ecNumber evidence="4">2.7.4.16</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metig_0857</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the HypE family.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.16" evidence="4"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96401.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799003.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0857"/>
    <dbReference type="EnsemblBacteria" id="AEF96401">
        <property type="protein sequence ID" value="AEF96401"/>
        <property type="gene ID" value="Metig_0857"/>
    </dbReference>
    <dbReference type="GeneID" id="10643698"/>
    <dbReference type="KEGG" id="mig:Metig_0857"/>
    <dbReference type="HOGENOM" id="CLU_049733_0_0_2"/>
    <dbReference type="OMA" id="CGNGGKE"/>
    <dbReference type="OrthoDB" id="34102at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009030">
        <property type="term" value="F:thiamine-phosphate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd02197">
        <property type="entry name" value="HypE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.650.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011854">
        <property type="entry name" value="HypE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010918">
        <property type="entry name" value="PurM-like_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036676">
        <property type="entry name" value="PurM-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016188">
        <property type="entry name" value="PurM-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036921">
        <property type="entry name" value="PurM-like_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30303">
        <property type="entry name" value="PTHR30303"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00586">
        <property type="entry name" value="AIRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02769">
        <property type="entry name" value="AIRS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005644">
        <property type="entry name" value="Hdrgns_mtr_HypE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55326">
        <property type="entry name" value="SSF55326"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56042">
        <property type="entry name" value="SSF56042"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02124">
        <property type="entry name" value="hypE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="AIRS" evidence="2">
        <location>
            <begin position="41"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="domain" description="AIRS_C" evidence="3">
        <location>
            <begin position="162"/>
            <end position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEF96401.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="334" mass="35699" checksum="7CF9F589B7217C32" modified="2011-07-27" version="1">MKITRMHGAGGKVMQDLIKEVILSNLEKTSVNGGIGLECLDDASTIPIGDKEIVFTVDGHTVKPIFFPGGDIGRLAVCGTVNDLAVMGAKPLALSLSLILPEGFEIEKLDKIMKSINEACKEADVAVITGDTKISNVDDIIISSAGIGIVDKGKAIRDSGMKDGDAIIVSGNIGEHGLAILLSRERFEFDADIKSDVAPLNKMIEMVLNEGIEVHAMKDPTRGGLANALNEMAEKSGLGIDIYEDKIPISEEVQSMADILGIDPLTVANEGKVVMAVKREDAKRCLEILRKHPLGKNAEIIGYATKEHKGVIMETIVGKRIIDTPIGDPIPRVC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>F6BD94</accession>
    <name>F6BD94_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">50S ribosomal protein L29</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">rpl29</name>
        <name evidence="6" type="ordered locus">Metig_0912</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the universal ribosomal protein uL29 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96455.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799057.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0912"/>
    <dbReference type="EnsemblBacteria" id="AEF96455">
        <property type="protein sequence ID" value="AEF96455"/>
        <property type="gene ID" value="Metig_0912"/>
    </dbReference>
    <dbReference type="GeneID" id="10643756"/>
    <dbReference type="KEGG" id="mig:Metig_0912"/>
    <dbReference type="HOGENOM" id="CLU_158491_2_2_2"/>
    <dbReference type="OMA" id="ELRDMTP"/>
    <dbReference type="OrthoDB" id="125807at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.310">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00374">
        <property type="entry name" value="Ribosomal_L29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001854">
        <property type="entry name" value="Ribosomal_L29/L35"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036049">
        <property type="entry name" value="Ribosomal_L29/L35_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018254">
        <property type="entry name" value="Ribosomal_L29_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00831">
        <property type="entry name" value="Ribosomal_L29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46561">
        <property type="entry name" value="SSF46561"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00012">
        <property type="entry name" value="L29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00579">
        <property type="entry name" value="RIBOSOMAL_L29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 6" id="KW-0689">Ribosomal protein</keyword>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="7"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEF96455.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="69" mass="7926" checksum="C6AD9CDA28AF7437" modified="2011-07-27" version="1">MAILRASEIREMSIEEMKEKLVELKKELLKENVHKAVSGAPSNPGRVKELKRTIARILTIMNEKKRQIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDD6</accession>
    <name>F6BDD6_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 4">Proteasome subunit alpha</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">20S proteasome alpha subunit</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="2">Proteasome core protein PsmA</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2" type="primary">psmA</name>
        <name evidence="7" type="ordered locus">Metig_0954</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 4">Component of the proteasome core, a large protease complex with broad specificity involved in protein degradation.</text>
    </comment>
    <comment type="activity regulation">
        <text evidence="2">The formation of the proteasomal ATPase PAN-20S proteasome complex, via the docking of the C-termini of PAN into the intersubunit pockets in the alpha-rings, triggers opening of the gate for substrate entry. Interconversion between the open-gate and close-gate conformations leads to a dynamic regulation of the 20S proteasome proteolysis activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 4">The 20S proteasome core is composed of 14 alpha and 14 beta subunits that assemble into four stacked heptameric rings, resulting in a barrel-shaped structure. The two inner rings, each composed of seven catalytic beta subunits, are sandwiched by two outer rings, each composed of seven alpha subunits. The catalytic chamber with the active sites is on the inside of the barrel. Has a gated structure, the ends of the cylinder being occluded by the N-termini of the alpha-subunits. Is capped at one or both ends by the proteasome regulatory ATPase, PAN.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 3 4">Belongs to the peptidase T1A family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96497.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799099.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0954"/>
    <dbReference type="EnsemblBacteria" id="AEF96497">
        <property type="protein sequence ID" value="AEF96497"/>
        <property type="gene ID" value="Metig_0954"/>
    </dbReference>
    <dbReference type="GeneID" id="10643800"/>
    <dbReference type="KEGG" id="mig:Metig_0954"/>
    <dbReference type="HOGENOM" id="CLU_035750_4_1_2"/>
    <dbReference type="OMA" id="FQVEYAR"/>
    <dbReference type="OrthoDB" id="57654at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019773">
        <property type="term" value="C:proteasome core complex, alpha-subunit complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004298">
        <property type="term" value="F:threonine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010498">
        <property type="term" value="P:proteasomal protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03756">
        <property type="entry name" value="proteasome_alpha_archeal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00289_A">
        <property type="entry name" value="Proteasome_A_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023332">
        <property type="entry name" value="Proteasome_alpha-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019982">
        <property type="entry name" value="Proteasome_asu_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000426">
        <property type="entry name" value="Proteasome_asu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10584">
        <property type="entry name" value="Proteasome_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00948">
        <property type="entry name" value="Proteasome_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="SSF56235"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03633">
        <property type="entry name" value="arc_protsome_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00388">
        <property type="entry name" value="PROTEASOME_ALPHA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51475">
        <property type="entry name" value="PROTEASOME_ALPHA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 2 3" id="KW-0647">Proteasome</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PROTEASOME_ALPHA_1" evidence="6">
        <location>
            <begin position="9"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="243"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS00388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEF96497.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="273" mass="30666" checksum="FA47715B65AD771F" modified="2011-07-27" version="1">MQMVPASAYDRAITVFSPEGRLYQVEYAREAVRRGTTAIGIKCKNGVVLAVDRRITSNLIEISSIEKIFQIDDHIAAATSGLVADARVLVDRARIEAQINRVTYGEPITVEALAKKICDIKQAYTQHGGARPFGVALLIAGIDRHSARLFETDPSGALIEYKATAIGSGRPIAMEILEKKYNENLTIEEAMELAIYVLSKTTEELKPENVDMVVIKDDEKMVERIPYEKIKELVDTVKEKIKQEKEKEKQEEENKEENGDNTEENNEKEENPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDG7</accession>
    <name>F6BDG7_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">2-amino-5-formylamino-6-ribosylaminopyrimidin-4(3H)-one 5'-monophosphate deformylase</fullName>
            <shortName evidence="6">FAPy deformylase</shortName>
            <ecNumber evidence="6">3.5.1.102</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Formamide hydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">arfB</name>
        <name evidence="7" type="ordered locus">Metig_0986</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes the hydrolysis of the formamide of 2-amino-5-formylamino-6-ribosylamino-4(3H)-pyrimidinone 5'-monophosphate (FAPy) to form 2,5-diamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate (APy).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>2-amino-5-formylamino-6-(5-phospho-D-ribosylamino)pyrimidin-4(3H)-one + H2O = 2,5-diamino-6-(1-D-ribosylamino)pyrimidin-4(3H)-one 5'-phosphate + formate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:27282"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:57258"/>
            <dbReference type="ChEBI" id="CHEBI:59545"/>
            <dbReference type="EC" id="3.5.1.102"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
        <cofactor evidence="6">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="6">Requires an additional second metal ion that could be Fe(2+) or Zn(2+).</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
        <text evidence="6">Requires one Fe(2+) ion for activity.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="6">Cofactor biosynthesis; coenzyme F420 biosynthesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="6">Cofactor biosynthesis; riboflavin biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the creatininase superfamily. FAPy deformylase family.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.102" evidence="6"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96528.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799130.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0986"/>
    <dbReference type="EnsemblBacteria" id="AEF96528">
        <property type="protein sequence ID" value="AEF96528"/>
        <property type="gene ID" value="Metig_0986"/>
    </dbReference>
    <dbReference type="GeneID" id="10643833"/>
    <dbReference type="KEGG" id="mig:Metig_0986"/>
    <dbReference type="HOGENOM" id="CLU_1192640_0_0_2"/>
    <dbReference type="OMA" id="FLIINCH"/>
    <dbReference type="OrthoDB" id="84020at2157"/>
    <dbReference type="UniPathway" id="UPA00071"/>
    <dbReference type="UniPathway" id="UPA00275"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043729">
        <property type="term" value="F:2-amino-5-formylamino-6-(5-phosphoribosylamino)pyrimidin-4(3H)-one formate-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008198">
        <property type="term" value="F:ferrous iron binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052645">
        <property type="term" value="P:F420-0 metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009231">
        <property type="term" value="P:riboflavin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10310">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02116">
        <property type="entry name" value="FAPy_deform"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024087">
        <property type="entry name" value="Creatininase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003785">
        <property type="entry name" value="Creatininase/forma_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024901">
        <property type="entry name" value="FAPy_deformylase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35005">
        <property type="entry name" value="PTHR35005"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02633">
        <property type="entry name" value="Creatininase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102215">
        <property type="entry name" value="SSF102215"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 6" id="KW-0408">Iron</keyword>
    <keyword evidence="2 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0862">Zinc</keyword>
    <feature type="binding site" evidence="6">
        <location>
            <position position="28"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="30"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="39"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="107"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEF96528.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="227" mass="25288" checksum="72F716A18FE70F15" modified="2011-07-27" version="1">MQLRLSSGKIIDEKVHEIGIIALGSFLENHGSALPIDTDAKIASYIALKTAILTGAKFLGVVIPSTEYHYVKHGIHNTPEEVVDFIKFLVKEGKKIGIKKFLIVNCHGGNVLIKGFLENLEKEFDVEIKMKNITFTHAATEEVSVGYVVGIVDENKLDEHTFNKYPEIGMVGLKEARENNKFIDEEAKVVEKEGVKIDKKLGEKILNQTIKECVEIVKLWVVKGNGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDH2</accession>
    <name>F6BDH2_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_0991</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96533.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799135.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0991"/>
    <dbReference type="EnsemblBacteria" id="AEF96533">
        <property type="protein sequence ID" value="AEF96533"/>
        <property type="gene ID" value="Metig_0991"/>
    </dbReference>
    <dbReference type="GeneID" id="10643838"/>
    <dbReference type="KEGG" id="mig:Metig_0991"/>
    <dbReference type="HOGENOM" id="CLU_094462_0_0_2"/>
    <dbReference type="OMA" id="PCFTDGN"/>
    <dbReference type="OrthoDB" id="92216at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF96533.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="217" mass="25844" checksum="C73E7A70E4A56DE7" modified="2011-07-27" version="1">MYEKIKNLFFEEIKSDDLLNLSENFYEDAREYLKNNDDEKEVERAKYYLRELRKLRIYKALYGDRTNLLEEEREILSLIEDIEYKKEEEVPKTEDLIEENVEDEVVGEDLGFDDTIEESYENFEDGENEEVGEDVSRDIENVNAQPKHINIKKDIDVVRVIHNFPAFTDGDYCYMLKKNDVVALNREIATILEKHNIVKKVNVYENEKKDKKILSIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDH3</accession>
    <name>F6BDH3_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DNA polymerase sliding clamp</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Proliferating cell nuclear antigen homolog</fullName>
            <shortName evidence="3">PCNA</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">pcn</name>
        <name evidence="8" type="ordered locus">Metig_0992</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Sliding clamp subunit that acts as a moving platform for DNA processing. Responsible for tethering the catalytic subunit of DNA polymerase and other proteins to DNA during high-speed replication.</text>
    </comment>
    <comment type="function">
        <text evidence="5">Sliding clamp subunit. Responsible for tethering the catalytic subunit of DNA polymerase to DNA during high-speed replication.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homotrimer. The subunits circularize to form a toroid; DNA passes through its center. Replication factor C (RFC) is required to load the toroid on the DNA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 3 4">Belongs to the PCNA family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96534.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799136.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0992"/>
    <dbReference type="EnsemblBacteria" id="AEF96534">
        <property type="protein sequence ID" value="AEF96534"/>
        <property type="gene ID" value="Metig_0992"/>
    </dbReference>
    <dbReference type="GeneID" id="10643839"/>
    <dbReference type="KEGG" id="mig:Metig_0992"/>
    <dbReference type="HOGENOM" id="CLU_043978_1_0_2"/>
    <dbReference type="OMA" id="TIRKDPN"/>
    <dbReference type="OrthoDB" id="70433at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030337">
        <property type="term" value="F:DNA polymerase processivity factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006275">
        <property type="term" value="P:regulation of DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00317">
        <property type="entry name" value="DNApol_clamp_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000730">
        <property type="entry name" value="Pr_cel_nuc_antig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022649">
        <property type="entry name" value="Pr_cel_nuc_antig_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022659">
        <property type="entry name" value="Pr_cel_nuc_antig_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022648">
        <property type="entry name" value="Pr_cel_nuc_antig_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11352:SF0">
        <property type="entry name" value="PTHR11352:SF0"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02747">
        <property type="entry name" value="PCNA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00705">
        <property type="entry name" value="PCNA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00339">
        <property type="entry name" value="PCNACYCLIN"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00590">
        <property type="entry name" value="pcna"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01251">
        <property type="entry name" value="PCNA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0235">DNA replication</keyword>
    <keyword evidence="2 3 4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PCNA_N" evidence="6">
        <location>
            <begin position="1"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="domain" description="PCNA_C" evidence="7">
        <location>
            <begin position="124"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEF96534.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="247" mass="27533" checksum="AFA09224C4BCC635" modified="2011-07-27" version="1">MFRAVISEAKEFKKVIDAASNLLDEICFEVNEEGIKARAMDPSHVALVSIDIPKEAFEEYEADVHDIGVDLEAFKKIMNRAKAKDKLILELDEEKNKLNVIFKGAATRKFALALYDVSSSNLQVPDIEYPNEILIKAGPFVDALKDADLVNDHVTLKVEDGKFIVYAKGDLNESETIFEEDSESIISMNIGENAKSTFNLEYLRDMTKAASSSDILKIYLGTDMPVKIEYDVAGANLTFLLAPRIES</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDK2</accession>
    <name>F6BDK2_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="7">TrkA-N domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Metig_1021</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Part of a potassium transport system.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96563.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799165.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1021"/>
    <dbReference type="EnsemblBacteria" id="AEF96563">
        <property type="protein sequence ID" value="AEF96563"/>
        <property type="gene ID" value="Metig_1021"/>
    </dbReference>
    <dbReference type="GeneID" id="10643875"/>
    <dbReference type="KEGG" id="mig:Metig_1021"/>
    <dbReference type="HOGENOM" id="CLU_046525_2_3_2"/>
    <dbReference type="OMA" id="RPSIVDY"/>
    <dbReference type="OrthoDB" id="116057at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015079">
        <property type="term" value="F:potassium ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006036">
        <property type="entry name" value="K_uptake_TrkA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006037">
        <property type="entry name" value="RCK_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036721">
        <property type="entry name" value="RCK_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003148">
        <property type="entry name" value="RCK_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02080">
        <property type="entry name" value="TrkA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02254">
        <property type="entry name" value="TrkA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00335">
        <property type="entry name" value="KUPTAKETRKA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF116726">
        <property type="entry name" value="SSF116726"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51202">
        <property type="entry name" value="RCK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51201">
        <property type="entry name" value="RCK_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0406">Ion transport</keyword>
    <keyword evidence="4" id="KW-0520">NAD</keyword>
    <keyword evidence="3" id="KW-0630">Potassium</keyword>
    <keyword evidence="2" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="RCK N-terminal" evidence="5">
        <location>
            <begin position="2"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="domain" description="RCK C-terminal" evidence="6">
        <location>
            <begin position="136"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEF96563.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="217" mass="23879" checksum="9C1DD9F20D648132" modified="2011-07-27" version="1">MYIIIAGIGRVGYTLAKSLSGKGYDLVLIDIDKEKCKTISAELDALVIHGDCTKTKILEDAGIEDADIFIAVTGKEEINLMSSLIAKDYGVAKTIARVSEPEYKDVFERLGVDVVISPELVAANYIEKLIERPGVVDLAIVGRGDAEILELVIPQNSKVANKKIKDLGRPKDYLIIAVYEDKELKIPDGNTELKPGERILVLVKSDSVDEIRKFFTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDL2</accession>
    <name>F6BDL2_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 9">Argininosuccinate synthase</fullName>
            <ecNumber evidence="2 9">6.3.4.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8 9">Citrulline--aspartate ligase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">argG</name>
        <name evidence="10" type="ordered locus">Metig_1031</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>ATP + L-aspartate + L-citrulline = 2-(N(omega)-L-arginino)succinate + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10932"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57472"/>
            <dbReference type="ChEBI" id="CHEBI:57743"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.4.5"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 9">Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 2/3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the argininosuccinate synthase family. Type 1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.5" evidence="2 9"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96573.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799175.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1031"/>
    <dbReference type="EnsemblBacteria" id="AEF96573">
        <property type="protein sequence ID" value="AEF96573"/>
        <property type="gene ID" value="Metig_1031"/>
    </dbReference>
    <dbReference type="GeneID" id="10643885"/>
    <dbReference type="KEGG" id="mig:Metig_1031"/>
    <dbReference type="HOGENOM" id="CLU_032784_4_0_2"/>
    <dbReference type="OMA" id="QCEVVTF"/>
    <dbReference type="OrthoDB" id="49443at2157"/>
    <dbReference type="UniPathway" id="UPA00068">
        <property type="reaction ID" value="UER00113"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004055">
        <property type="term" value="F:argininosuccinate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006526">
        <property type="term" value="P:arginine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01999">
        <property type="entry name" value="Argininosuccinate_Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00005">
        <property type="entry name" value="Arg_succ_synth_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001518">
        <property type="entry name" value="Arginosuc_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018223">
        <property type="entry name" value="Arginosuc_synth_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023434">
        <property type="entry name" value="Arginosuc_synth_type_1_subfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024074">
        <property type="entry name" value="AS_cat/multimer_dom_body"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11587">
        <property type="entry name" value="PTHR11587"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00764">
        <property type="entry name" value="Arginosuc_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69864">
        <property type="entry name" value="SSF69864"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00032">
        <property type="entry name" value="argG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00564">
        <property type="entry name" value="ARGININOSUCCIN_SYN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00565">
        <property type="entry name" value="ARGININOSUCCIN_SYN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="3 9" id="KW-0055">Arginine biosynthesis</keyword>
    <keyword evidence="7 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 9 10" id="KW-0436">Ligase</keyword>
    <keyword evidence="6 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="9"/>
            <end position="17"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="87"/>
        </location>
        <ligand>
            <name>L-citrulline</name>
            <dbReference type="ChEBI" id="CHEBI:57743"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="117"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="119"/>
        </location>
        <ligand>
            <name>L-aspartate</name>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="123"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="124"/>
        </location>
        <ligand>
            <name>L-aspartate</name>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="127"/>
        </location>
        <ligand>
            <name>L-citrulline</name>
            <dbReference type="ChEBI" id="CHEBI:57743"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="174"/>
        </location>
        <ligand>
            <name>L-citrulline</name>
            <dbReference type="ChEBI" id="CHEBI:57743"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="183"/>
        </location>
        <ligand>
            <name>L-citrulline</name>
            <dbReference type="ChEBI" id="CHEBI:57743"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="258"/>
        </location>
        <ligand>
            <name>L-citrulline</name>
            <dbReference type="ChEBI" id="CHEBI:57743"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="270"/>
        </location>
        <ligand>
            <name>L-citrulline</name>
            <dbReference type="ChEBI" id="CHEBI:57743"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00029916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AEF96573.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="398" mass="44870" checksum="0498304DFFAEBE68" modified="2011-07-27" version="1">MSEKIAVLAYSGGLDTSCCLKLLEDHYGYKVVSACVDVGQPEEELKEPEEKAKKLGVLEHYTIDAKEEFARDYIFRAIKANAMYEGYPLSTALARPLIALKIVELAKKIGAEAVAHGCTGKGNDQFRFETVIRIKAPELKIIAPIRDLNLTRKEEIEYAKQKGIPIPTESKSFSIDENLWGRSIEGGVLEDPMVEPPEEAFAWTKNPKECTEEEIVEIEFENGVPVAINGEKLEPVELIKKANEIAGRNGVGRIDIIEDRIIGLKSRETYECPGAVMLITAHKALEQLVLTRDELRFKEIVDSLYGELIYRGLWFDPLREDLDAFIDKTQERVTGKVKLKLYAGSLRVIGRESPYALYSESLVSFDDKELDQREIIGMVKYHGLQAALYEKVKNKLQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDM5</accession>
    <name>F6BDM5_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">DNA binding protein, Tfx family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1045</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96586.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799188.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1045"/>
    <dbReference type="EnsemblBacteria" id="AEF96586">
        <property type="protein sequence ID" value="AEF96586"/>
        <property type="gene ID" value="Metig_1045"/>
    </dbReference>
    <dbReference type="GeneID" id="10643899"/>
    <dbReference type="KEGG" id="mig:Metig_1045"/>
    <dbReference type="HOGENOM" id="CLU_125807_0_1_2"/>
    <dbReference type="OMA" id="VKVPYST"/>
    <dbReference type="OrthoDB" id="96651at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription, initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1190.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007630">
        <property type="entry name" value="RNA_pol_sigma70_r4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029291">
        <property type="entry name" value="Tfx_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004645">
        <property type="entry name" value="Tfx_DNA-bd_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018384">
        <property type="entry name" value="Tfx_DNA-bd_euryarc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036657">
        <property type="entry name" value="Tfx_DNA-bd_sf_arc"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04545">
        <property type="entry name" value="Sigma70_r4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14601">
        <property type="entry name" value="TFX_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004932">
        <property type="entry name" value="DNA_bind_Tfx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89915">
        <property type="entry name" value="SSF89915"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00721">
        <property type="entry name" value="tfx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH cro/C1-type" evidence="1">
        <location>
            <begin position="27"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96586.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="143" mass="16675" checksum="BFCBAC10B2C45607" modified="2011-07-27" version="1">MDLEKKKKNFAKSFLTEMQIKVLELRRKGLTQEEIAKMLGTSRANISMIEKRAKENVEKAKNTLRIYNQIFAPLVINIEKDTDVLKIPEIVFKKADEKGIHVKYSTLDILEFIRKNAKNYVEKRVVKESFKIYVLENGELDIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDN2</accession>
    <name>F6BDN2_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_1052</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96593.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799194.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1052"/>
    <dbReference type="EnsemblBacteria" id="AEF96593">
        <property type="protein sequence ID" value="AEF96593"/>
        <property type="gene ID" value="Metig_1052"/>
    </dbReference>
    <dbReference type="GeneID" id="10643906"/>
    <dbReference type="KEGG" id="mig:Metig_1052"/>
    <dbReference type="HOGENOM" id="CLU_1269936_0_0_2"/>
    <dbReference type="OMA" id="CGWACPG"/>
    <dbReference type="OrthoDB" id="92246at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12801">
        <property type="entry name" value="Fer4_5"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="58"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="103"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="155"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="2">
        <location>
            <begin position="58"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="2">
        <location>
            <begin position="159"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF96593.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="217" mass="25035" checksum="B3B3A0E70711DC3B" modified="2011-07-27" version="1">MDLKFKRILSLIVKYGVFIVAIPTLAIVILRCPYVIPFLACDMCPVVDCPSKYHRREFVVFALGYIALFRKDFCAWVCPYGTLNDIIYKIRRKLSKKTFKIPLELKLSLNFLKYVVFVFAIIAILNGNPRYYVPLHTSSLITSIKLAFMTAGNAYYTRLALILVGLLLGIVIPRFWCRFLCPYGTTFQLIKKGLRKLKNGYKNEHKCEKCSKCGNYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDN5</accession>
    <name>F6BDN5_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Isopropylmalate/citramalate/homocitrate synthase</fullName>
            <ecNumber evidence="7">2.3.1.182</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Metig_1057</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="4">Amino-acid biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the alpha-IPM synthase/homocitrate synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.182" evidence="7"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96596.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799197.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1057"/>
    <dbReference type="EnsemblBacteria" id="AEF96596">
        <property type="protein sequence ID" value="AEF96596"/>
        <property type="gene ID" value="Metig_1057"/>
    </dbReference>
    <dbReference type="GeneID" id="10643911"/>
    <dbReference type="KEGG" id="mig:Metig_1057"/>
    <dbReference type="HOGENOM" id="CLU_022158_0_1_2"/>
    <dbReference type="OMA" id="NTMRMLV"/>
    <dbReference type="OrthoDB" id="10632at2157"/>
    <dbReference type="UniPathway" id="UPA00098">
        <property type="reaction ID" value="UER00358"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043714">
        <property type="term" value="F:(R)-citramalate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003852">
        <property type="term" value="F:2-isopropylmalate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009098">
        <property type="term" value="P:leucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013709">
        <property type="entry name" value="2-isopropylmalate_synth_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002034">
        <property type="entry name" value="AIPM/Hcit_synth_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011830">
        <property type="entry name" value="LEU1_arch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036230">
        <property type="entry name" value="LeuA_allosteric_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000891">
        <property type="entry name" value="PYR_CT"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00682">
        <property type="entry name" value="HMGL-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08502">
        <property type="entry name" value="LeuA_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00917">
        <property type="entry name" value="LeuA_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110921">
        <property type="entry name" value="SSF110921"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02090">
        <property type="entry name" value="LEU1_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00815">
        <property type="entry name" value="AIPM_HOMOCIT_SYNTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00816">
        <property type="entry name" value="AIPM_HOMOCIT_SYNTH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50991">
        <property type="entry name" value="PYR_CT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="3" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="3" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Pyruvate carboxyltransferase" evidence="6">
        <location>
            <begin position="24"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00029440"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEF96596.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="518" mass="56217" checksum="650C0ABF8A1BFF19" modified="2011-07-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDV0</accession>
    <name>F6BDV0_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1122</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96661.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1122"/>
    <dbReference type="EnsemblBacteria" id="AEF96661">
        <property type="protein sequence ID" value="AEF96661"/>
        <property type="gene ID" value="Metig_1122"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_1122"/>
    <dbReference type="HOGENOM" id="CLU_2490475_0_0_2"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="57"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96661.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="86" mass="9513" checksum="770D90D08140264F" modified="2011-07-27" version="1">MKVLYMITIFLFIFGFISSLMLNINTNSNNNSKVGNTPNFKLASAPLTLILTNNLKLIFLMLAGAITFGLSTFINLIAHVHSRLPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDW9</accession>
    <name>F6BDW9_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcriptional regulator, ArsR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1141</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96680.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1141"/>
    <dbReference type="EnsemblBacteria" id="AEF96680">
        <property type="protein sequence ID" value="AEF96680"/>
        <property type="gene ID" value="Metig_1141"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_1141"/>
    <dbReference type="HOGENOM" id="CLU_670165_0_0_2"/>
    <dbReference type="OMA" id="LHYEFKL"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041664">
        <property type="entry name" value="AAA_16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001845">
        <property type="entry name" value="HTH_ArsR_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13191">
        <property type="entry name" value="AAA_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01022">
        <property type="entry name" value="HTH_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00418">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50987">
        <property type="entry name" value="HTH_ARSR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH arsR-type" evidence="1">
        <location>
            <begin position="319"/>
            <end position="408"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96680.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="408" mass="46007" checksum="BB4F000D9E50C335" modified="2011-07-27" version="1">MLMLDPIDFIYNSASATKNAMHRLKLKTNPFSEKPIRGNTKFFVGRTSELREIADIFGAAQYGSVVNAAIVGTKGIGKSSLLNIIYYAAKKQGHWAVELTASQITPRQFLIQVLYNLIRDNLISIDGTICSEYIQHSQKIMEIYRKLVDYGDRTPVHYPRERIERDLDFLLNEVKEEDKLCIILVDEADQFAKKSCLSLLQFFHSFLYEDGILAFFAGSPTLMDDLTKISPAMRDRIPKIINMPPLSKEESYDLIRRRLEDAHIGNAKEFDPFTDAAIDKIVEECDGIPRKIIMTCSESISIALKKGVSKIDEDIVKMAMEVLGISVGHQILNHLTPAQSKIVRALAELGGSSTVTELAKYLGNSPGTIGTHLSDIYEMGYIYKERRGNNVYYTLSKELMDVLMNDND</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-06-02" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDX1</accession>
    <name>F6BDX1_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_1143</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96682.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799283.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96682">
        <property type="protein sequence ID" value="AEF96682"/>
        <property type="gene ID" value="Metig_1143"/>
    </dbReference>
    <dbReference type="GeneID" id="10644001"/>
    <dbReference type="KEGG" id="mig:Metig_1143"/>
    <dbReference type="HOGENOM" id="CLU_800796_0_0_2"/>
    <dbReference type="OMA" id="YRAKKAW"/>
    <dbReference type="OrthoDB" id="42050at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF96682.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="356" mass="42387" checksum="484E2C7D57155896" modified="2011-07-27" version="1">MKIIYNSPEDFLTEKLWLIPTIRENTLELNENGTFEVVNNIPIIRSLATPSLSKTISRLIYLIKDKMVMFKKIEKANWLKNLFNYIHEKYFDKSNNNYYRAKKAWYRMIGDLFEDIKNWNFKKVRGKVISLLRVLSPILVFDVHNITKWNHTKTFVKFIEELTKNDIVVIIRCPIEAIDYAKKLFKNAKINTIAAVKYYAKHLGYYISNRVAKYLAEISKGNLNVIRLILIHSKKELKTLRDIKIPWLKILPKIVPKKYEKIVEIACELRKFKIKDIEKRVDLKLPTIYEYLNDLVDMGIISKTKIRKTIRFSIKLDREKLIKLLDKLPRKPILLLFTINNILEPIPRLEKFRVFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDY5</accession>
    <name>F6BDY5_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Ammonium transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Metig_1157</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ammonia transporter channel (TC 1.A.11.2) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96696.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799297.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1157"/>
    <dbReference type="EnsemblBacteria" id="AEF96696">
        <property type="protein sequence ID" value="AEF96696"/>
        <property type="gene ID" value="Metig_1157"/>
    </dbReference>
    <dbReference type="GeneID" id="10644015"/>
    <dbReference type="KEGG" id="mig:Metig_1157"/>
    <dbReference type="HOGENOM" id="CLU_000445_33_1_2"/>
    <dbReference type="OMA" id="CAGSDVM"/>
    <dbReference type="OrthoDB" id="66605at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005887">
        <property type="term" value="C:integral component of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008519">
        <property type="term" value="F:ammonium transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3430.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029020">
        <property type="entry name" value="Ammonium/urea_transptr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018047">
        <property type="entry name" value="Ammonium_transpt_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024041">
        <property type="entry name" value="NH4_transpt_AmtB-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002229">
        <property type="entry name" value="RhesusRHD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00909">
        <property type="entry name" value="Ammonium_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00342">
        <property type="entry name" value="RHESUSRHD"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01219">
        <property type="entry name" value="AMMONIUM_TRANSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0924">Ammonia transport</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="40"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="81"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="108"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="146"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="186"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="217"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="249"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="298"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="332"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="domain" description="Ammonium_transp" evidence="9">
        <location>
            <begin position="8"/>
            <end position="380"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AEF96696.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="383" mass="39951" checksum="73879925901C487F" modified="2011-07-27" version="1">MEGIDVFFFLWAASLIFFMKAGFIALEIGQFSHRNAAYHCVLKLLDLAAVFIGYLAIGYGISYGLENIIPLVFGGFDVELGAWFMKMVMFAAAAVTIITGGVAERIKILPYFIGALIVGGILYPIVEHLVWGGGFEALGIAFHDYAGSGAVHLFGGLVGLMAAWALGPRAKKYINGIPQALPGHNIPLAVLGAFILAFGWYGFNIGSAASVGDGLELTRVALATTMALAGGIIGGALSSRNDPLYTANGMCAGLVAVCSGVDLFTPIGALIVGLLAGLQQPFTFKFIEEKLKIDDVCAIGPVHAMSGLIGVICAGIPFLLRDPSATSFVGQVLGAVIISLIAIIGGAILYKGLDATIGLRVSKEVEEVGLDEGVLQVSAYTND</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDY9</accession>
    <name>F6BDY9_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">DNA primase small subunit PriS</fullName>
            <ecNumber evidence="10">2.7.7.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">priS</name>
        <name evidence="13" type="ordered locus">Metig_1161</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalytic subunit of DNA primase, an RNA polymerase that catalyzes the synthesis of short RNA molecules used as primers for DNA polymerase during DNA replication. The small subunit contains the primase catalytic core and has DNA synthesis activity on its own. Binding to the large subunit stabilizes and modulates the activity, increasing the rate of DNA synthesis while decreasing the length of the DNA fragments, and conferring RNA synthesis capability. The DNA polymerase activity may enable DNA primase to also catalyze primer extension after primer synthesis. May also play a role in DNA repair.</text>
    </comment>
    <comment type="function">
        <text evidence="12">RNA polymerase that catalyzes the synthesis of short RNA molecules used as primers for DNA polymerase during DNA replication.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="10">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="10">Heterodimer of a small subunit (PriS) and a large subunit (PriL).</text>
    </comment>
    <comment type="similarity">
        <text evidence="10 11">Belongs to the eukaryotic-type primase small subunit family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.-" evidence="10"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96700.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799301.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1161"/>
    <dbReference type="EnsemblBacteria" id="AEF96700">
        <property type="protein sequence ID" value="AEF96700"/>
        <property type="gene ID" value="Metig_1161"/>
    </dbReference>
    <dbReference type="GeneID" id="10644021"/>
    <dbReference type="KEGG" id="mig:Metig_1161"/>
    <dbReference type="HOGENOM" id="CLU_056123_0_0_2"/>
    <dbReference type="OMA" id="GYHVHVR"/>
    <dbReference type="OrthoDB" id="95578at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990077">
        <property type="term" value="C:primosome complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003896">
        <property type="term" value="F:DNA primase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04860">
        <property type="entry name" value="AE_Prim_S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00700">
        <property type="entry name" value="DNA_primase_sml_arc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002755">
        <property type="entry name" value="DNA_primase_S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014052">
        <property type="entry name" value="DNA_primase_ssu_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023639">
        <property type="entry name" value="DNA_primase_ssu_PriS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10536">
        <property type="entry name" value="PTHR10536"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01896">
        <property type="entry name" value="DNA_primase_S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00335">
        <property type="entry name" value="primase_sml"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10 11" id="KW-0235">DNA replication</keyword>
    <keyword evidence="1 10 11" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="7 10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9 10" id="KW-0464">Manganese</keyword>
    <keyword evidence="6 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 10" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="2 10 11" id="KW-0639">Primosome</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 10" id="KW-0804">Transcription</keyword>
    <keyword evidence="3 10 11" id="KW-0808">Transferase</keyword>
    <feature type="active site" evidence="10">
        <location>
            <position position="97"/>
        </location>
    </feature>
    <feature type="active site" evidence="10">
        <location>
            <position position="99"/>
        </location>
    </feature>
    <feature type="active site" evidence="10">
        <location>
            <position position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU004224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AEF96700.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="345" mass="41181" checksum="412EE98EE6FE0EFE" modified="2011-07-27" version="1">MDKLNEIQNLYKEYYSYAIKNDLLEVPEGIEYREFGYGYFKKVDNRNISFKDEEEYKNWVLKNAPFHLYKSLSYMEFPDKSGGANKKKVFRREIAFDIDVHKTNKCRHSDDWVCDKCLNAAKEQAIYLIEEFLMPDFGLSKEDLQIAFSGNRGYHIYIKPKDEEIRNIIEHYEKPQRRFFIEYILGKNLNLNKVGSGWRRRLVKEFKKKRIATKQFEKEENWKKIIEKRKNKDKIYSIINETLNKLELDEKVMDDDIRLLRVINSLHGYTGFIVKEIKYKELDNFNPLKDAIFNGFNKKFYNVKIKQKVDKINIGDEIYSTKSKEIPASVLLFLFGHGVDFEIIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDZ3</accession>
    <name>F6BDZ3_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Cell division ATPase MinD</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Metig_1166</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ParA family. MinD subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96704.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799304.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1166"/>
    <dbReference type="EnsemblBacteria" id="AEF96704">
        <property type="protein sequence ID" value="AEF96704"/>
        <property type="gene ID" value="Metig_1166"/>
    </dbReference>
    <dbReference type="GeneID" id="10644026"/>
    <dbReference type="KEGG" id="mig:Metig_1166"/>
    <dbReference type="HOGENOM" id="CLU_037612_0_3_2"/>
    <dbReference type="OMA" id="CESAKAY"/>
    <dbReference type="OrthoDB" id="105856at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002586">
        <property type="entry name" value="CobQ/CobB/MinD/ParA_Nub-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010224">
        <property type="entry name" value="MinD_archaea"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025501">
        <property type="entry name" value="MinD_FleN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01656">
        <property type="entry name" value="CbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003092">
        <property type="entry name" value="MinD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01969">
        <property type="entry name" value="minD_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="5" id="KW-0132">Cell division</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CbiA" evidence="4">
        <location>
            <begin position="5"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEF96704.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="261" mass="27771" checksum="E50A744924346C23" modified="2011-07-27" version="1">MTVTIAIASGKGGTGKTTIAANLAVALSRFGKNVIVLDADIAMANLELILGLEGKPVTLNDVLAGTADIEEAIYEGPGGVKVVPAGISLERFRRANPERLEEVLTKLNEKADILIIDCPAGLGKDALVALSAAENLIVVVNPEISSISDALKIIAVARRLETNVLGAIINRVSNDSTELSAKAIETILEIPIMGIIPEDPNVRRSAAFGEPIVLRYPDSPAAQAIMEIAAKLVGKQYIPEKKEKESFIKRFIKGLFGRKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>F6BE01</accession>
    <name>F6BE01_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Diphthine synthase</fullName>
            <ecNumber evidence="6">2.1.1.98</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Diphthamide biosynthesis methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">dphB</name>
        <name evidence="9" type="ordered locus">Metig_1174</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">S-adenosyl-L-methionine-dependent methyltransferase that catalyzes the trimethylation of the amino group of the modified target histidine residue in translation elongation factor 2 (EF-2), to form an intermediate called diphthine. The three successive methylation reactions represent the second step of diphthamide biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>2-[(3S)-amino-3-carboxypropyl]-L-histidyl-[translation elongation factor 2] + 3 S-adenosyl-L-methionine = diphthine-[translation elongation factor 2] + 3 H(+) + 3 S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:36415"/>
            <dbReference type="Rhea" id="RHEA-COMP:9749"/>
            <dbReference type="Rhea" id="RHEA-COMP:10172"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:73995"/>
            <dbReference type="ChEBI" id="CHEBI:82696"/>
            <dbReference type="EC" id="2.1.1.98"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 6">Protein modification; peptidyl-diphthamide biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the diphthine synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.98" evidence="6"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96712.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799312.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1174"/>
    <dbReference type="EnsemblBacteria" id="AEF96712">
        <property type="protein sequence ID" value="AEF96712"/>
        <property type="gene ID" value="Metig_1174"/>
    </dbReference>
    <dbReference type="GeneID" id="10644034"/>
    <dbReference type="KEGG" id="mig:Metig_1174"/>
    <dbReference type="HOGENOM" id="CLU_066040_0_0_2"/>
    <dbReference type="OMA" id="TAGDPMV"/>
    <dbReference type="OrthoDB" id="102360at2157"/>
    <dbReference type="UniPathway" id="UPA00559"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004164">
        <property type="term" value="F:diphthine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017183">
        <property type="term" value="P:peptidyl-diphthamide biosynthetic process from peptidyl-histidine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd11647">
        <property type="entry name" value="DHP5_DphB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.950.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1010.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01084">
        <property type="entry name" value="Diphthine_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000878">
        <property type="entry name" value="4pyrrol_Mease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035996">
        <property type="entry name" value="4pyrrol_Methylase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014777">
        <property type="entry name" value="4pyrrole_Mease_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014776">
        <property type="entry name" value="4pyrrole_Mease_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004551">
        <property type="entry name" value="Dphthn_synthase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10882">
        <property type="entry name" value="PTHR10882"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00590">
        <property type="entry name" value="TP_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036432">
        <property type="entry name" value="Diphthine_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53790">
        <property type="entry name" value="SSF53790"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00522">
        <property type="entry name" value="dph5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6 9" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6 7" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4 6 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="TP_methylase" evidence="8">
        <location>
            <begin position="1"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="region of interest" description="S-adenosyl-L-methionine binding" evidence="6 7">
        <location>
            <begin position="113"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6 7">
        <location>
            <position position="9"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6 7">
        <location>
            <position position="85"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6 7">
        <location>
            <position position="88"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6 7">
        <location>
            <position position="164"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6 7">
        <location>
            <position position="207"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6 7">
        <location>
            <position position="232"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR036432-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AEF96712.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="255" mass="28907" checksum="A1BA3A50B24A5E8C" modified="2011-07-27" version="1">MLILAGLGLYDEKDMPLKTLEFAKKVDKIYAEFYTAVLTGTTIEKIEETLGRKIEVLSREKVEYETEKLIEEAKEKDIMFLTAGDPMVATTHVDLAVEAKKRGIDVIIINAPSIYSAVGITGLQLYKFGKTTSIVFPEPNYFPETPYDVIKENLKMGYHTLCLLDIHADKNKFMTANEGLKILLELEKRRKENVITENTKVVVVARAGSLKPKLVYGKIKDLINYDFGKPLHCIIIPGKLHFMEEEALKYLCENV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>F6BE21</accession>
    <name>F6BE21_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Formylmethanofuran dehydrogenase subunit B</fullName>
            <ecNumber evidence="2">1.2.99.5</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1194</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.2.99.5" evidence="2"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96732.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799331.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1194"/>
    <dbReference type="EnsemblBacteria" id="AEF96732">
        <property type="protein sequence ID" value="AEF96732"/>
        <property type="gene ID" value="Metig_1194"/>
    </dbReference>
    <dbReference type="GeneID" id="10644054"/>
    <dbReference type="KEGG" id="mig:Metig_1194"/>
    <dbReference type="HOGENOM" id="CLU_034348_0_0_2"/>
    <dbReference type="OMA" id="TAEVCHG"/>
    <dbReference type="OrthoDB" id="11387at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018493">
        <property type="term" value="F:formylmethanofuran dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015948">
        <property type="term" value="P:methanogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02761">
        <property type="entry name" value="MopB_FmdB-FwdB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016457">
        <property type="entry name" value="Formylmethanofuran_DH_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006656">
        <property type="entry name" value="Mopterin_OxRdtase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00384">
        <property type="entry name" value="Molybdopterin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005646">
        <property type="entry name" value="FwdB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03129">
        <property type="entry name" value="one_C_dehyd_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Molybdopterin" evidence="1">
        <location>
            <begin position="50"/>
            <end position="428"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96732.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="434" mass="48511" checksum="9019FCC9267B8440" modified="2011-07-27" version="1">MEIVKDVVCPFCGTLCDDIICYVENNHIVGTKNACRIGHSKFTHRDGAVRYTEPLMRENKKDDFKKVDWDTAIEKTAEILVNAKRPILYGFSATECHAHAYGMKLAEMVGGVVSNTAEVCHGPSVWALQDVGYPICTLGEVKNRADVVIYWGCNPMHAHPRHMGRYGVFARGFFRERGRSDRTLIVVDPRKTDTAKLADIHLQVEQHKDYELISAMRAVLKDFELQVDKVAGIPVELIYEAVDILKNAQFGILFFGMGVTQSKSKHRNIDNAIQLVVDLNAYTKFSLIPMRGHYNVNGFNQVCTWVSGFPLCVDYSRGYPRFNPGDTSVTDLLIRKEADAMLNIASDPGAHFPNKAVEGMAEIPLIAIEPHRTPTTELANIILPPAIAGVECEGTAYRMDGVPIELKKVIEPPEGVLSDREIMKKIIRKVKELL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>F6BE38</accession>
    <name>F6BE38_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Amidophosphoribosyltransferase</fullName>
            <shortName evidence="6">ATase</shortName>
            <ecNumber evidence="6">2.4.2.14</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Glutamine phosphoribosylpyrophosphate amidotransferase</fullName>
            <shortName evidence="6">GPATase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">purF</name>
        <name evidence="12" type="ordered locus">Metig_0012</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes the formation of phosphoribosylamine from phosphoribosylpyrophosphate (PRPP) and glutamine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 7">
            <text>5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate = 5-phospho-alpha-D-ribose 1-diphosphate + H2O + L-glutamine</text>
            <dbReference type="Rhea" id="RHEA:14905"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:58681"/>
            <dbReference type="EC" id="2.4.2.14"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6 9">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="6 9">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6 10">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="6 10">Binds 1 [4Fe-4S] cluster per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 6 7">Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 6 7">In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.14" evidence="6"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95574.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798184.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0012"/>
    <dbReference type="MEROPS" id="C44.001"/>
    <dbReference type="EnsemblBacteria" id="AEF95574">
        <property type="protein sequence ID" value="AEF95574"/>
        <property type="gene ID" value="Metig_0012"/>
    </dbReference>
    <dbReference type="GeneID" id="10642847"/>
    <dbReference type="KEGG" id="mig:Metig_0012"/>
    <dbReference type="HOGENOM" id="CLU_022389_3_1_2"/>
    <dbReference type="OMA" id="ENAQPTF"/>
    <dbReference type="OrthoDB" id="37299at2157"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00124"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004044">
        <property type="term" value="F:amidophosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006541">
        <property type="term" value="P:glutamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009113">
        <property type="term" value="P:purine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00715">
        <property type="entry name" value="GPATase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01931">
        <property type="entry name" value="PurF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017932">
        <property type="entry name" value="GATase_2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRibTrfase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005854">
        <property type="entry name" value="PurF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035584">
        <property type="entry name" value="PurF_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13537">
        <property type="entry name" value="GATase_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00156">
        <property type="entry name" value="Pribosyltran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000485">
        <property type="entry name" value="Amd_phspho_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="SSF53271"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="SSF56235"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01134">
        <property type="entry name" value="purF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51278">
        <property type="entry name" value="GATASE_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="6" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="6 7 12" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="6 10" id="KW-0408">Iron</keyword>
    <keyword evidence="6 10" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="6 9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 6 7" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glutamine amidotransferase type-2" evidence="11">
        <location>
            <begin position="2"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="6 8">
        <location>
            <position position="2"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6 10">
        <location>
            <position position="258"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6 9">
        <location>
            <position position="305"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6 9">
        <location>
            <position position="367"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6 9">
        <location>
            <position position="368"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6 10">
        <location>
            <position position="404"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6 10">
        <location>
            <position position="453"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6 10">
        <location>
            <position position="456"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR000485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000485-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000485-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000485-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AEF95574.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="470" mass="52268" checksum="BB57FF7ACEB3DBDB" modified="2011-07-27" version="1">MCGIFGVYSNERINVAKKIYYGLFALQHRGQEGAGIATSDGRGFHHHKGIGLVSEVFTNNELQSLYGYIGIGHVRYSTTGDSTIENCQPFVVKSSFGPIAIAHNGDIVNSTILKRELERMGHIFISSTDSEVIAQLLVRELLKTEDIIEAIKNTAKRLIGAYSLLIMFNDKLIAVRDPNGFKPLCIGRDENCIYFASENCALDTVNAEFVRDIKPGEIVMVDDGEIKCYEIEKPCEEVNTELNLPVNDCIYNNCVSTCMFEYVYFARPDSIIDGISVYEVRRKIGRILAKEHPCDADIVSPVPDSGITFALGYSEELGIPYYEGLIKNRYVGRTFILPTQEERDLAVRLKLNPVKSILKDKKVVLVDDSIVRGTTSRRIVNMLKKAGAKEVHLRIGSPRIISPCFYGIDMATKEELIASSKTDEEICKYVNADSLGYLSIEGLVKAIGRKDLCLACVSGCYPTDVSCKFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F6BE78</accession>
    <name>F6BE78_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Carbohydrate kinase-related protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_1217</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96755.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799354.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1217"/>
    <dbReference type="EnsemblBacteria" id="AEF96755">
        <property type="protein sequence ID" value="AEF96755"/>
        <property type="gene ID" value="Metig_1217"/>
    </dbReference>
    <dbReference type="GeneID" id="10644077"/>
    <dbReference type="KEGG" id="mig:Metig_1217"/>
    <dbReference type="HOGENOM" id="CLU_979811_0_0_2"/>
    <dbReference type="OMA" id="HYIKPKI"/>
    <dbReference type="OrthoDB" id="45436at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052855">
        <property type="term" value="F:ADP-dependent NAD(P)H-hydrate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000631">
        <property type="entry name" value="CARKD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01256">
        <property type="entry name" value="Carb_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="SSF53613"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF96755.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="273" mass="29857" checksum="C014BD97E258E85F" modified="2011-07-27" version="1">MIIAGTMPIKGLELIRGKPTLKGNKIIINNKEFPISMGTGALIGAVLKTLEYFGEDKLYVITAGDIGEGDGSLKIYDALKDVDDDLLIIHYIKPKISKIKEIDFSPKIIADAGGMYVAKAANIGDKFYLFLPDVGELAFLADEKASHPAYVRGFISEVDDKEVPELIKRAYEKSKMPKYMVVKGEKDYVVEDGDIVETIDAPKIEAMECIGGTGDTLTGIISSLIYCGYKTKDACVLGCKINRMLGEVANAKPNTQVDELIHNIPKVLKHLNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>F6BE82</accession>
    <name>F6BE82_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">50S ribosomal protein L23</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">rpl23</name>
        <name evidence="8" type="ordered locus">Metig_1222</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Binds to 23S rRNA. One of the proteins that surrounds the polypeptide exit tunnel on the outside of the ribosome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Part of the 50S ribosomal subunit. Contacts protein L29.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6 7">Belongs to the universal ribosomal protein uL23 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96759.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799358.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1222"/>
    <dbReference type="EnsemblBacteria" id="AEF96759">
        <property type="protein sequence ID" value="AEF96759"/>
        <property type="gene ID" value="Metig_1222"/>
    </dbReference>
    <dbReference type="GeneID" id="10644083"/>
    <dbReference type="KEGG" id="mig:Metig_1222"/>
    <dbReference type="HOGENOM" id="CLU_037562_4_2_2"/>
    <dbReference type="OMA" id="MDPYKVI"/>
    <dbReference type="OrthoDB" id="113971at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01369_A">
        <property type="entry name" value="Ribosomal_L23_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01369_B">
        <property type="entry name" value="Ribosomal_L23_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019985">
        <property type="entry name" value="Ribosomal_L23"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012678">
        <property type="entry name" value="Ribosomal_L23/L15e_core_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001014">
        <property type="entry name" value="Ribosomal_L23/L25_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013025">
        <property type="entry name" value="Ribosomal_L25/23"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11620">
        <property type="entry name" value="PTHR11620"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00276">
        <property type="entry name" value="Ribosomal_L23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54189">
        <property type="entry name" value="SSF54189"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03636">
        <property type="entry name" value="uL23_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00050">
        <property type="entry name" value="RIBOSOMAL_L23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6 7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 6 7" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3 6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2 6" id="KW-0699">rRNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEF96759.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="86" mass="9942" checksum="F6763F57D02DFDF2" modified="2011-07-27" version="1">MDAFDVIKMPVISEKTMRLIEEENKLVFYVHRKATKEDIKRAMKELFNVEVEKVNTMITPKGEKKAYVKLKDGYDASEVAANLGIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>F6BE93</accession>
    <name>F6BE93_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Methyl-coenzyme M reductase subunit beta</fullName>
            <ecNumber evidence="8">2.8.4.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Coenzyme-B sulfoethylthiotransferase beta</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">Metig_1233</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Component of the methyl-coenzyme M reductase (MCR) I that catalyzes the reductive cleavage of methyl-coenzyme M (CoM-S-CH3 or 2-(methylthio)ethanesulfonate) using coenzyme B (CoB or 7-mercaptoheptanoylthreonine phosphate) as reductant which results in the production of methane and the mixed heterodisulfide of CoB and CoM (CoM-S-S-CoB). This is the final step in methanogenesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>coenzyme B + methyl-coenzyme M = coenzyme M-coenzyme B heterodisulfide + methane</text>
            <dbReference type="Rhea" id="RHEA:12532"/>
            <dbReference type="ChEBI" id="CHEBI:16183"/>
            <dbReference type="ChEBI" id="CHEBI:58286"/>
            <dbReference type="ChEBI" id="CHEBI:58411"/>
            <dbReference type="ChEBI" id="CHEBI:58596"/>
            <dbReference type="EC" id="2.8.4.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="1">
            <dbReference type="Rhea" id="RHEA:12533"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>coenzyme F430</name>
            <dbReference type="ChEBI" id="CHEBI:60540"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4 8">One-carbon metabolism; methyl-coenzyme M reduction; methane from methyl-coenzyme M: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Hexamer of two alpha, two beta, and two gamma chains.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">MCR is a hexamer of two alpha, two beta, and two gamma chains, forming a dimer of heterotrimers.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the methyl-coenzyme M reductase beta subunit family.</text>
    </comment>
    <dbReference type="EC" id="2.8.4.1" evidence="8"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96770.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799369.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1233"/>
    <dbReference type="EnsemblBacteria" id="AEF96770">
        <property type="protein sequence ID" value="AEF96770"/>
        <property type="gene ID" value="Metig_1233"/>
    </dbReference>
    <dbReference type="GeneID" id="10644096"/>
    <dbReference type="KEGG" id="mig:Metig_1233"/>
    <dbReference type="HOGENOM" id="CLU_617682_0_0_2"/>
    <dbReference type="OMA" id="TAMFEMG"/>
    <dbReference type="OrthoDB" id="25508at2157"/>
    <dbReference type="UniPathway" id="UPA00646">
        <property type="reaction ID" value="UER00699"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050524">
        <property type="term" value="F:coenzyme-B sulfoethylthiotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015948">
        <property type="term" value="P:methanogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.840.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.470">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008924">
        <property type="entry name" value="Me_CoM_Rdtase_asu/bsu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015823">
        <property type="entry name" value="Me_CoM_Rdtase_asu_N_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003179">
        <property type="entry name" value="Me_CoM_Rdtase_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022679">
        <property type="entry name" value="Me_CoM_Rdtase_bsu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022680">
        <property type="entry name" value="Me_CoM_Rdtase_bsu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009024">
        <property type="entry name" value="Me_CoM_Rdtase_Fd-like_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02241">
        <property type="entry name" value="MCR_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02783">
        <property type="entry name" value="MCR_beta_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000263">
        <property type="entry name" value="Meth_CoM_rd_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48081">
        <property type="entry name" value="SSF48081"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55088">
        <property type="entry name" value="SSF55088"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03257">
        <property type="entry name" value="met_CoM_red_bet"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0484">Methanogenesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="MCR_beta_N" evidence="10">
        <location>
            <begin position="6"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="domain" description="MCR_beta" evidence="9">
        <location>
            <begin position="189"/>
            <end position="437"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000951"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00002461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00011155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR000263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AEF96770.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="444" mass="47546" checksum="A56E89C2AD5295CE" modified="2011-07-27" version="1">MVKYEDKINLYDAKGNLVEENVPLEAISPLHNPTIQKIVKDIKRTVAVNLAGIENALKTGAVGGKACIIPGRTLDLPIVENAETIMDYVEKLLRVSPDDDTNLKLINGGKQMAVQLPSKRLEVAAEYSVSMLNTAMALKEAIIKTFDVDMFDAPMVHAAILGRYPQVPDYMGANIASLLGAPTNLEGLGYALRNIMVNHYVATTKKNIMNAVAFASIMEQTAMFEMGDAIGVFERLHLLGLAYQGLNADNLVIDLVKANGKNGTVGTVVASVVERALEDGVITEDKKLPSGFVMYKPVDVAKWNAYAAAGLVAAVIVNCGAARAAQNVASTILYYNDILEYETGLPGVDFGRAEGTAVGFSFFSHSIYGGGGPGIFHGNHVVTRHSKGFAIPPVCAAMCVDAGTQMFSPEKTSALVGAVYSAIDEFREPLKYVIEGALEIKDKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEE4</accession>
    <name>F6BEE4_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Aminotransferase</fullName>
            <ecNumber evidence="1">2.6.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_0044</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.-" evidence="1"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95605.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798214.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0044"/>
    <dbReference type="EnsemblBacteria" id="AEF95605">
        <property type="protein sequence ID" value="AEF95605"/>
        <property type="gene ID" value="Metig_0044"/>
    </dbReference>
    <dbReference type="GeneID" id="10642879"/>
    <dbReference type="KEGG" id="mig:Metig_0044"/>
    <dbReference type="HOGENOM" id="CLU_017584_4_3_2"/>
    <dbReference type="OMA" id="SVAMTGW"/>
    <dbReference type="OrthoDB" id="32104at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009058">
        <property type="term" value="P:biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004838">
        <property type="entry name" value="NHTrfase_class1_PyrdxlP-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00105">
        <property type="entry name" value="AA_TRANSFER_CLASS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotran_1_2" evidence="2">
        <location>
            <begin position="25"/>
            <end position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF95605.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="375" mass="41993" checksum="FD87CAD71940C9FB" modified="2011-07-27" version="1">MISDRCKNIKPSAIREIFNMANETSINLGIGEPDFDTPKHIVEAAKKALDEGRTHYTPNNGILKLREAICEKLKKDNNLDVNPNNVIVTCGASEALMLSIMALVNKGDEVLIPNPGFVSYFSLTELCEGKIKSVDLDENFNIDVDELNEKISNKTKCIILNSPSNPTGKVLDKDIIKGICEIAEDKGIIIISDEIYEKIIYDKKHYSPMQFTDNCILINGFSKAYAMTGWRIGYLAVSESLNKKYNLIENMIKIHQYGFACATSFAQYGALAALKGDQKCVEDMVKEFKRRRDLIVNGLKKFFKVYKPEGAFYVFPDVSEYGDGVEVAKKLIENGILCVPGIAFGENGKNYVRFSYATKYEDIEKALQIMDSVFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEH8</accession>
    <name>F6BEH8_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Putative fluoride ion transporter CrcB</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">crcB</name>
        <name evidence="7" type="ordered locus">Metig_0078</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Important for reducing fluoride concentration in the cell, thus reducing its toxicity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cell membrane</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the CrcB (TC 9.B.71) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95639.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798248.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0078"/>
    <dbReference type="EnsemblBacteria" id="AEF95639">
        <property type="protein sequence ID" value="AEF95639"/>
        <property type="gene ID" value="Metig_0078"/>
    </dbReference>
    <dbReference type="GeneID" id="10642913"/>
    <dbReference type="KEGG" id="mig:Metig_0078"/>
    <dbReference type="HOGENOM" id="CLU_114342_3_0_2"/>
    <dbReference type="OMA" id="FLMFESF"/>
    <dbReference type="OrthoDB" id="112898at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005887">
        <property type="term" value="C:integral component of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903425">
        <property type="term" value="F:fluoride transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00454">
        <property type="entry name" value="CrcB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003691">
        <property type="entry name" value="CrcB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28259">
        <property type="entry name" value="PTHR28259"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02537">
        <property type="entry name" value="CRCB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00494">
        <property type="entry name" value="crcB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="38"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="63"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="91"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEF95639.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="123" mass="13355" checksum="F0287A23D35689C8" modified="2011-07-27" version="1">MRELLLIGIGGFIGAILRYVIGGIVPVKFGLPTGTLTVNLIGSFILGFFMYSSLFYISSDAKFFIATGFCGALTTFSTFSYETFTLIEEGLLVKAIANILINVLGCLFMVYVGRTFSLMVFNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2020-06-17" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEL5</accession>
    <name>F6BEL5_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_0115</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95676.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF95676">
        <property type="protein sequence ID" value="AEF95676"/>
        <property type="gene ID" value="Metig_0115"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0115"/>
    <dbReference type="HOGENOM" id="CLU_2875077_0_0_2"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF95676.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="63" mass="6985" checksum="835CEFBDBBD52F62" modified="2011-07-27" version="1">MSVTGMETDDMFPMNPEGTEADDIQVQRGHLSMIVHVGQTPVKCHSKHQFTTTNLILSSIKIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEM9</accession>
    <name>F6BEM9_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 5">50S ribosomal protein L31e</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">rpl31e</name>
        <name evidence="6" type="ordered locus">Metig_1289</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL31 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the ribosomal protein L31e family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96826.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1289"/>
    <dbReference type="EnsemblBacteria" id="AEF96826">
        <property type="protein sequence ID" value="AEF96826"/>
        <property type="gene ID" value="Metig_1289"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_1289"/>
    <dbReference type="HOGENOM" id="CLU_112570_3_2_2"/>
    <dbReference type="OMA" id="MEERIYT"/>
    <dbReference type="OrthoDB" id="115384at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00463">
        <property type="entry name" value="Ribosomal_L31e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.440.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00410">
        <property type="entry name" value="Ribosomal_L31e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000054">
        <property type="entry name" value="Ribosomal_L31e"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020052">
        <property type="entry name" value="Ribosomal_L31e_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023621">
        <property type="entry name" value="Ribosomal_L31e_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10956">
        <property type="entry name" value="PTHR10956"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01198">
        <property type="entry name" value="Ribosomal_L31e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01380">
        <property type="entry name" value="Ribosomal_L31e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54575">
        <property type="entry name" value="SSF54575"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01144">
        <property type="entry name" value="RIBOSOMAL_L31E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 5 6" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00029527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00410"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEF96826.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="82" mass="9781" checksum="0A651FB22F579FF8" modified="2011-07-27" version="1">MEERIYTIPLRDIMNKSKRTKRAPRAIRKIREFLKRHMKAEIVKLDNSINEKVWERSIEKVPPRIRVKAVKEGDVVRATLAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEV2</accession>
    <name>F6BEV2_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 8 10">1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase</fullName>
            <ecNumber evidence="5 8 10">5.3.1.16</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">hisA</name>
        <name evidence="11" type="ordered locus">Metig_0128</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>1-(5-phospho-beta-D-ribosyl)-5-[(5-phospho-beta-D-ribosylamino)methylideneamino]imidazole-4-carboxamide = 5-[(5-phospho-1-deoxy-D-ribulos-1-ylimino)methylamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide</text>
            <dbReference type="Rhea" id="RHEA:15469"/>
            <dbReference type="ChEBI" id="CHEBI:58435"/>
            <dbReference type="ChEBI" id="CHEBI:58525"/>
            <dbReference type="EC" id="5.3.1.16"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 8 10">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 4/9.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 8 10">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 8 9">Belongs to the HisA/HisF family.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.16" evidence="5 8 10"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95688.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798297.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0128"/>
    <dbReference type="EnsemblBacteria" id="AEF95688">
        <property type="protein sequence ID" value="AEF95688"/>
        <property type="gene ID" value="Metig_0128"/>
    </dbReference>
    <dbReference type="GeneID" id="10642963"/>
    <dbReference type="KEGG" id="mig:Metig_0128"/>
    <dbReference type="HOGENOM" id="CLU_048577_1_1_2"/>
    <dbReference type="OMA" id="EWLHLVD"/>
    <dbReference type="OrthoDB" id="89881at2157"/>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00009"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003949">
        <property type="term" value="F:1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino]imidazole-4-carboxamide isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04732">
        <property type="entry name" value="HisA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01014">
        <property type="entry name" value="HisA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006062">
        <property type="entry name" value="His_biosynth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006063">
        <property type="entry name" value="HisA_bact_arch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044524">
        <property type="entry name" value="Isoase_HisA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023016">
        <property type="entry name" value="Isoase_HisA-like_bact"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43090">
        <property type="entry name" value="PTHR43090"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00977">
        <property type="entry name" value="His_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="SSF51366"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00007">
        <property type="entry name" value="TIGR00007"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="8 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 9" id="KW-0368">Histidine biosynthesis</keyword>
    <keyword evidence="7 8 10" id="KW-0413">Isomerase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Proton acceptor" evidence="8">
        <location>
            <position position="8"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="8">
        <location>
            <position position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000901"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00018464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AEF95688.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="240" mass="26234" checksum="ACE3CCB3E238ACF6" modified="2011-07-27" version="1">MIVIPAVDIKDQKCVQLIQGDPSKKHVELDNPVEVAKRWEEEGAKMLHLVDLDGAFEGRRKNKEILKKIIDEVNIPVEIGGGIRTIEDAMELIDIGAERIIIGTVAVQNPNFVEELSKKVDSKRIMVALDAKDGYVVIKGWKEKTKYTPVEMGKILQEKGAGSILFTNVNVEGLLKGIDVNPIKELVDSLDIPIVASGGVSSIDDLMKLKECGVEGVVVGSALYKGLIDLKEAIKVVEGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEV4</accession>
    <name>F6BEV4_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 9">Quinolinate synthase</fullName>
            <ecNumber evidence="2 9">2.5.1.72</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">nadA</name>
        <name evidence="10" type="ordered locus">Metig_0130</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the condensation of iminoaspartate with dihydroxyacetone phosphate to form quinolinate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>dihydroxyacetone phosphate + iminosuccinate = H(+) + 2 H2O + phosphate + quinolinate</text>
            <dbReference type="Rhea" id="RHEA:25888"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29959"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
            <dbReference type="ChEBI" id="CHEBI:77875"/>
            <dbReference type="EC" id="2.5.1.72"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="9">Binds 1 [4Fe-4S] cluster per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 9">Cofactor biosynthesis; NAD(+) biosynthesis; quinolinate from iminoaspartate: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the quinolinate synthase family. Type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.72" evidence="2 9"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95690.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798299.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0130"/>
    <dbReference type="EnsemblBacteria" id="AEF95690">
        <property type="protein sequence ID" value="AEF95690"/>
        <property type="gene ID" value="Metig_0130"/>
    </dbReference>
    <dbReference type="GeneID" id="10642965"/>
    <dbReference type="KEGG" id="mig:Metig_0130"/>
    <dbReference type="HOGENOM" id="CLU_047382_0_0_2"/>
    <dbReference type="OMA" id="CFCSTMN"/>
    <dbReference type="OrthoDB" id="18897at2157"/>
    <dbReference type="UniPathway" id="UPA00253">
        <property type="reaction ID" value="UER00327"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008987">
        <property type="term" value="F:quinolinate synthetase A activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009435">
        <property type="term" value="P:NAD biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10800">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00568">
        <property type="entry name" value="NadA_type2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003473">
        <property type="entry name" value="NadA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036094">
        <property type="entry name" value="NadA_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023066">
        <property type="entry name" value="Quinolinate_synth_type2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30573">
        <property type="entry name" value="PTHR30573"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02445">
        <property type="entry name" value="NadA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF142754">
        <property type="entry name" value="SSF142754"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00550">
        <property type="entry name" value="nadA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 9" id="KW-0408">Iron</keyword>
    <keyword evidence="8 9" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="6 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 9" id="KW-0662">Pyridine nucleotide biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9 10" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Iminoaspartate binding" evidence="9">
        <location>
            <begin position="111"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Iminoaspartate binding" evidence="9">
        <location>
            <begin position="198"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="22"/>
        </location>
        <ligand>
            <name>iminosuccinate</name>
            <dbReference type="ChEBI" id="CHEBI:77875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="39"/>
        </location>
        <ligand>
            <name>iminosuccinate</name>
            <dbReference type="ChEBI" id="CHEBI:77875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="85"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="128"/>
        </location>
        <ligand>
            <name>iminosuccinate</name>
            <dbReference type="ChEBI" id="CHEBI:77875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="172"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="215"/>
        </location>
        <ligand>
            <name>iminosuccinate</name>
            <dbReference type="ChEBI" id="CHEBI:77875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="264"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AEF95690.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="308" mass="35203" checksum="8176C23C8B1BCB43" modified="2011-07-27" version="1">MSDIVQRINQLKEEKNAVILAHNYQREEIQRIADFVGDSLELCIKAKETDADIIVFCGVDFMAETAKILNSDKKVLIPEIKDIECPMAHQLPAEVVRRAKEEHPDAMVVVYVNTLAETKALADATCTSANADRVVNSLDCDKVLFGPDRNLAYFVSKRTNKEIIPIPEDGHCYVHKKFTVEDVKRVRREYPNAELLVHPECDPEVQDLADYVLSTGGMIRHVLNSDKKEFIIGTECELITRIKLELEKTGEEKVLIPLRKDAICDPMKRITLEKVERCLREEKYEITLDEEIIKKARIAIERMLSVKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEW7</accession>
    <name>F6BEW7_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein family UPF0066</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metig_0143</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the tRNA methyltransferase O family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95703.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798312.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0143"/>
    <dbReference type="EnsemblBacteria" id="AEF95703">
        <property type="protein sequence ID" value="AEF95703"/>
        <property type="gene ID" value="Metig_0143"/>
    </dbReference>
    <dbReference type="GeneID" id="10642978"/>
    <dbReference type="KEGG" id="mig:Metig_0143"/>
    <dbReference type="HOGENOM" id="CLU_013458_2_2_2"/>
    <dbReference type="OMA" id="KIFEIGM"/>
    <dbReference type="OrthoDB" id="117039at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd09281">
        <property type="entry name" value="UPF0066"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023370">
        <property type="entry name" value="TrmO-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023368">
        <property type="entry name" value="UPF0066_cons_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040372">
        <property type="entry name" value="YaeB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036413">
        <property type="entry name" value="YaeB-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036414">
        <property type="entry name" value="YaeB_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12818">
        <property type="entry name" value="PTHR12818"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01980">
        <property type="entry name" value="TrmO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118196">
        <property type="entry name" value="SSF118196"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00104">
        <property type="entry name" value="tRNA_TsaA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01318">
        <property type="entry name" value="TSAA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51668">
        <property type="entry name" value="TSAA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="TsaA-like" evidence="3">
        <location>
            <begin position="5"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00033753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEF95703.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="127" mass="14875" checksum="8535308B8E4023D1" modified="2011-07-27" version="1">MEFRVFPIGKIKQRTNNTILEIFNEFKDGLDDLNEGNRILVFVWFDKSDTPNKRKILKVHPKGNINNPIRGVFSTRSPIRPNPIGIYNVKILKMENNRLYVEKIDAFDETPIIDIKIFSKELDCPEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEX9</accession>
    <name>F6BEX9_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_0157</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95715.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0157"/>
    <dbReference type="EnsemblBacteria" id="AEF95715">
        <property type="protein sequence ID" value="AEF95715"/>
        <property type="gene ID" value="Metig_0157"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0157"/>
    <dbReference type="HOGENOM" id="CLU_1259105_0_0_2"/>
    <dbReference type="OMA" id="CPYECQI"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030815">
        <property type="entry name" value="Arch_SelU_Cterm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR04569">
        <property type="entry name" value="arch_SelU_Cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF95715.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="221" mass="26293" checksum="98831221C5C446C7" modified="2011-07-27" version="1">MVILMIIFGLFGKTGCGKTEILRELKKHHPVVDIEECANTRGSILGDLYHLKQRSQEEFEKELYKQIKKAEEKGYCVVEYEGRKIGGVKKLKIPELFANLNNYTYKILVDCPYECQINRLLNQYLPKNEHEKRILISKFELLRNSLKREEILRTIDKIIELIEKDEYYNAALLIEEKLYREHYMRVIKKIQPDLIVYNEDLDKSVKIVDEFIKSKLKHVDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F6BF19</accession>
    <name>F6BF19_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Pyruvate ferredoxin/flavodoxin oxidoreductase, delta subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metig_1354</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="2">Heterotetramer of one alpha, one beta, one delta and one gamma chain.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96889.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799486.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1354"/>
    <dbReference type="EnsemblBacteria" id="AEF96889">
        <property type="protein sequence ID" value="AEF96889"/>
        <property type="gene ID" value="Metig_1354"/>
    </dbReference>
    <dbReference type="GeneID" id="10644217"/>
    <dbReference type="KEGG" id="mig:Metig_1354"/>
    <dbReference type="HOGENOM" id="CLU_139698_1_1_2"/>
    <dbReference type="OMA" id="GSWRTFK"/>
    <dbReference type="OrthoDB" id="125632at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016625">
        <property type="term" value="F:oxidoreductase activity, acting on the aldehyde or oxo group of donors, iron-sulfur protein as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011898">
        <property type="entry name" value="PorD_KorD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43724">
        <property type="entry name" value="PTHR43724"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02179">
        <property type="entry name" value="PorD_KorD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="3">
        <location>
            <begin position="25"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="3">
        <location>
            <begin position="55"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011595"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEF96889.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="85" mass="9514" checksum="BEDACE2B8D21D9A8" modified="2011-07-27" version="1">MVTIGAIIYEPGSSIRNKTGSWRVFRPILDADKCIKCENCYIFCPEGAIQEVDGKFTINYDYCKGCLICVNECPVNAITKVREGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>F6BF29</accession>
    <name>F6BF29_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_1364</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96899.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799496.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1364"/>
    <dbReference type="EnsemblBacteria" id="AEF96899">
        <property type="protein sequence ID" value="AEF96899"/>
        <property type="gene ID" value="Metig_1364"/>
    </dbReference>
    <dbReference type="GeneID" id="10644228"/>
    <dbReference type="KEGG" id="mig:Metig_1364"/>
    <dbReference type="HOGENOM" id="CLU_1691544_0_0_2"/>
    <dbReference type="OMA" id="SMLVIMD"/>
    <dbReference type="OrthoDB" id="95579at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010254">
        <property type="entry name" value="B12-dep_deHydtase_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012019">
        <property type="entry name" value="HcgB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11576">
        <property type="entry name" value="HcgB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005018">
        <property type="entry name" value="UCP005018"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF96899.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="163" mass="18065" checksum="DA4C3FFCAC42D0E5" modified="2011-07-27" version="1">MENLIKKAYTQSIKNKRRGDEEEEVEAIKRIILNAKKIIIATNNQKKFKVISDILSRVVNAKIEMLNINTNCADLTRMPAVSKGLIAVDTEEADVVIARGRLGVPGSGSMLVIMDGKGRILSASLSPSSVVHKEDIETRIKKEIIDALKRVGIELKTDDLRKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>F6BF31</accession>
    <name>F6BF31_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Radical SAM core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Metig_1366</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96901.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799498.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1366"/>
    <dbReference type="EnsemblBacteria" id="AEF96901">
        <property type="protein sequence ID" value="AEF96901"/>
        <property type="gene ID" value="Metig_1366"/>
    </dbReference>
    <dbReference type="GeneID" id="10644230"/>
    <dbReference type="KEGG" id="mig:Metig_1366"/>
    <dbReference type="HOGENOM" id="CLU_060920_0_0_2"/>
    <dbReference type="OMA" id="NAGFTCP"/>
    <dbReference type="OrthoDB" id="26475at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.30.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039661">
        <property type="entry name" value="ELP3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaB/NifB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032432">
        <property type="entry name" value="Radical_SAM_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023404">
        <property type="entry name" value="rSAM_horseshoe"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005911">
        <property type="entry name" value="YhcC-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11135">
        <property type="entry name" value="PTHR11135"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11135:SF1">
        <property type="entry name" value="PTHR11135:SF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16199">
        <property type="entry name" value="Radical_SAM_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01091">
        <property type="entry name" value="uncharacterized_CHP01210-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01212">
        <property type="entry name" value="TIGR01212"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="7">
        <location>
            <begin position="27"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEF96901.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="317" mass="37211" checksum="47E0A099DD76B787" modified="2011-07-27" version="1">MIDKSIIDEMYREGYRVAQYGIYTKKSKPYKTFKIPVDAGFSCPNKNNDDSGCIFCPKMGRPIAVKYCNIKYPLKEQIEMQMKKQKSKGIEKFYIYFYPGTNTYADLETLKELWDLALSYDDVIGLSIGTRPDCLEKEKLDILEEYVKEGYEIWIDLGIQTMHQKTLELLNRKHNVSDTIRAIKECKKRNILVCGHVILGLPNETWSEMMETARILSILEVDALKIYPLVVVKNTKLEEMYWRGEYRALDEKQYINLVCDFLEHLSPYVLIQRISKDKVPEDIKVSPEWSLSRLRILNEVNKELERRNSRQGAKYNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>F6BF36</accession>
    <name>F6BF36_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Diphthami_syn_2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1371</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96906.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799502.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1371"/>
    <dbReference type="EnsemblBacteria" id="AEF96906">
        <property type="protein sequence ID" value="AEF96906"/>
        <property type="gene ID" value="Metig_1371"/>
    </dbReference>
    <dbReference type="GeneID" id="10644236"/>
    <dbReference type="KEGG" id="mig:Metig_1371"/>
    <dbReference type="HOGENOM" id="CLU_010289_0_2_2"/>
    <dbReference type="OMA" id="NYALYWA"/>
    <dbReference type="OrthoDB" id="101215at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd01994">
        <property type="entry name" value="Alpha_ANH_like_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002761">
        <property type="entry name" value="Diphthami_syn_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030662">
        <property type="entry name" value="DPH6/MJ0570"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005237">
        <property type="entry name" value="MJ0570"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022427">
        <property type="entry name" value="MJ0570_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12196">
        <property type="entry name" value="PTHR12196"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01902">
        <property type="entry name" value="Diphthami_syn_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039123">
        <property type="entry name" value="Diphthamide_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03679">
        <property type="entry name" value="arCOG00187"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00290">
        <property type="entry name" value="MJ0570_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00289">
        <property type="entry name" value="TIGR00289"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Diphthami_syn_2" evidence="1">
        <location>
            <begin position="1"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96906.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="222" mass="25400" checksum="A383CD23B22BAA31" modified="2011-07-27" version="1">MKVASLYSGGKDSTYALWWALHQGWEVKYLVNVISKNKESYMFHIPNVELTDLISESVGIPLVRVYTKGEKEKEVLDLKKALEKLDIDGIVCGALASEYQKTRIDHICEEIGIKSFAPLWHKDQELILRDVAKFFDVRIVGVSAYGLGKEWLGKRITEENIDKLLKICERYQINKAFEGGEAETFVFDAPFFKKKIEVVDYEIIWDGVSGVYIVKDAKLVDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>F6BF43</accession>
    <name>F6BF43_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Acetyl-CoA decarbonylase/synthase complex subunit beta</fullName>
            <shortName evidence="9">ACDS complex subunit beta</shortName>
            <ecNumber evidence="9">2.3.1.169</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">ACDS complex acyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">cdhC</name>
        <name evidence="11" type="ordered locus">Metig_1378</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Part of a complex that catalyzes the reversible cleavage of acetyl-CoA, allowing autotrophic growth from CO(2). The alpha-epsilon complex generates CO from CO(2), while the beta subunit (this protein) combines the CO with CoA and a methyl group to form acetyl-CoA. The methyl group, which is incorporated into acetyl-CoA, is transferred to the beta subunit by a corrinoid iron-sulfur protein (the gamma-delta complex).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>acetyl-CoA + Co(I)-[corrinoid Fe-S protein] + H(+) = CO + CoA + methyl-Co(III)-[corrinoid Fe-S protein]</text>
            <dbReference type="Rhea" id="RHEA:45212"/>
            <dbReference type="Rhea" id="RHEA-COMP:11110"/>
            <dbReference type="Rhea" id="RHEA-COMP:11111"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17245"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:85033"/>
            <dbReference type="ChEBI" id="CHEBI:85035"/>
            <dbReference type="EC" id="2.3.1.169"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>[Ni-Fe-S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:60400"/>
        </cofactor>
        <text evidence="9">Binds 1 [Ni-Fe-S] cluster.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8 9">Monomer. The ACDS complex is made up of alpha, epsilon, beta, gamma and delta chains with a probable stoichiometry of (alpha(2)epsilon(2))(4)-beta(8)-(gamma(1)delta(1))(8).</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the CdhC family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.169" evidence="9"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96913.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799509.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1378"/>
    <dbReference type="EnsemblBacteria" id="AEF96913">
        <property type="protein sequence ID" value="AEF96913"/>
        <property type="gene ID" value="Metig_1378"/>
    </dbReference>
    <dbReference type="GeneID" id="10644251"/>
    <dbReference type="KEGG" id="mig:Metig_1378"/>
    <dbReference type="HOGENOM" id="CLU_613408_0_0_2"/>
    <dbReference type="OMA" id="YIEGIMH"/>
    <dbReference type="OrthoDB" id="26992at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016407">
        <property type="term" value="F:acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018492">
        <property type="term" value="F:carbon-monoxide dehydrogenase (acceptor) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043884">
        <property type="term" value="F:CO-methylating acetyl-CoA synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016151">
        <property type="term" value="F:nickel cation binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006084">
        <property type="term" value="P:acetyl-CoA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1650.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01138">
        <property type="entry name" value="CdhC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045822">
        <property type="entry name" value="ACS_CODH_B_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004461">
        <property type="entry name" value="CO_DH/Ac-CoA_synth_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038571">
        <property type="entry name" value="CO_DH/Ac-CoA_synth_bsu_3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023432">
        <property type="entry name" value="CO_DH/Ac-CoA_synth_bsu_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011254">
        <property type="entry name" value="Prismane-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42281">
        <property type="entry name" value="PTHR42281"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19436">
        <property type="entry name" value="ACS_CODH_B_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03598">
        <property type="entry name" value="CdhC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56821">
        <property type="entry name" value="SSF56821"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00316">
        <property type="entry name" value="cdhC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9 11" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5 9" id="KW-0408">Iron</keyword>
    <keyword evidence="6 9" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 9" id="KW-0533">Nickel</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="ACS_CODH_B_C" evidence="10">
        <location>
            <begin position="165"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="186"/>
        </location>
        <ligand>
            <name>[Ni-Fe-S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:60400"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="189"/>
        </location>
        <ligand>
            <name>[Ni-Fe-S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:60400"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="275"/>
        </location>
        <ligand>
            <name>[Ni-Fe-S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:60400"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="277"/>
        </location>
        <ligand>
            <name>[Ni-Fe-S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:60400"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00025865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF19436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AEF96913.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="464" mass="51873" checksum="B070CB87E9D62002" modified="2011-07-27" version="1">MFDDIPVSVGPMNEGERVRGPDMYVELAGPKSYGFELVRVGEASDKVEVIGKDINEMEEGTRTPFAIIVRVSGDNLEEDLEGVLERRIHEFFNYIEGIMHLNQRDSVWIRINKSSASKGLTLEHIGKVIQRLYKAEFPFIKSVDVTLITDPDKVKEELEKAREVYAKRDARARELSEEDVDVFYGCIMCQSFAPTHVCIITPDKPALCGGITYFDARAAAKIDPEGPIFEVPKGELLDEKLGIYSGVNKVVSEKSQGSIEEIALHSVMYKPSTSCGCFEAITFYIPEVDGFGIVHRDFKGETPMGVPFSSLAGQCSGGKQVEGFCGMCIEYMRSPKFFQADGGWQRVVWLPKDLKEKVKDAIPEDLYDKIATEEDVSNTEELAKFLKEKNHPVVERWGKVEEEEIEEEEFVEEAEEGFAVPTLELPAGFGGLPPGIKIILHNAVIKAEKIIITKEEPSKKSSKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>F6BF56</accession>
    <name>F6BF56_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0282 protein Metig_1391</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1391</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0282 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96926.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799522.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1391"/>
    <dbReference type="EnsemblBacteria" id="AEF96926">
        <property type="protein sequence ID" value="AEF96926"/>
        <property type="gene ID" value="Metig_1391"/>
    </dbReference>
    <dbReference type="GeneID" id="10644264"/>
    <dbReference type="KEGG" id="mig:Metig_1391"/>
    <dbReference type="HOGENOM" id="CLU_079268_0_0_2"/>
    <dbReference type="OMA" id="YHYDHHT"/>
    <dbReference type="OrthoDB" id="36408at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01406">
        <property type="entry name" value="UPF0282"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014426">
        <property type="entry name" value="UPF0282_hydrls"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43546:SF4">
        <property type="entry name" value="PTHR43546:SF4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004944">
        <property type="entry name" value="UCP004944_hydrls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="SSF56281"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96926.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="291" mass="34013" checksum="21FF96CC2EF0067A" modified="2011-07-27" version="1">MKIIPIASESLGVRSLATYVETEDVKVLIDPGVAIAPKRYGLPPNEIEFEQLRKIREKLNNFAKECDIITISHYHYDHYTPFHDDEYLDSKDYAKTLYKDKILLIKHPTEFINRSQMKRAKVFLENVKNIAKEINYADNKTFRFKNTQIKFSPPFPHGKDAKLGYVLLTTIKDKNFTFLHASDVQGVIFDELKDYIINECPDLIIMSGPPTYLINRFGKENLKKVDENLIDILKNTDAQLILDHHFLRDKNYKEKLSVDFKTIAEYIGEENYLLEAYRKDIKDGKQINFKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>F6BF62</accession>
    <name>F6BF62_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Exodeoxyribonuclease V</fullName>
            <ecNumber evidence="3">3.1.11.5</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_1397</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.1.11.5" evidence="3"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96932.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799528.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1397"/>
    <dbReference type="EnsemblBacteria" id="AEF96932">
        <property type="protein sequence ID" value="AEF96932"/>
        <property type="gene ID" value="Metig_1397"/>
    </dbReference>
    <dbReference type="GeneID" id="10644270"/>
    <dbReference type="KEGG" id="mig:Metig_1397"/>
    <dbReference type="HOGENOM" id="CLU_006651_0_0_2"/>
    <dbReference type="OMA" id="FYWEHLG"/>
    <dbReference type="OrthoDB" id="43490at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008854">
        <property type="term" value="F:exodeoxyribonuclease V activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029493">
        <property type="entry name" value="RecD-like_HHH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027785">
        <property type="entry name" value="UvrD-like_helicase_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14490">
        <property type="entry name" value="HHH_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13538">
        <property type="entry name" value="UvrD_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA" evidence="2">
        <location>
            <begin position="532"/>
            <end position="682"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="492"/>
            <end position="519"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF96932.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="1101" mass="130030" checksum="9A9782F3A724C223" modified="2011-07-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-09-29" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>F6BA97</accession>
    <name>F6BA97_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="3">AAA ATPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_0230</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the archaeal ATPase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95787.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798396.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF95787">
        <property type="protein sequence ID" value="AEF95787"/>
        <property type="gene ID" value="Metig_0230"/>
    </dbReference>
    <dbReference type="GeneID" id="10643065"/>
    <dbReference type="KEGG" id="mig:Metig_0230"/>
    <dbReference type="HOGENOM" id="CLU_068608_0_0_2"/>
    <dbReference type="OMA" id="VWNAIKI"/>
    <dbReference type="OrthoDB" id="50712at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011579">
        <property type="entry name" value="ATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01637">
        <property type="entry name" value="ATPase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA" evidence="2">
        <location>
            <begin position="21"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF95787.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="349" mass="41252" checksum="554E166DA60E1F47" modified="2011-07-27" version="1">MNLFNREKEMKEILSIIEFEPNFIYFIYGPINSGKTALINEIINNRLDKDKYVVFYINLRRYFISKYGDFVEVLFKEYEENKKPIELVKGLIKDTPMLFGIPIPKNTLETLLSKKEEKNIFDYITNILLEIKEDGKQPIIILDELQKIGDLKINGFLIYELFNYFISLTKELHLCHVFCLSSDSLFIEKVYSEAILNGRAKYLLVDDFDKETAIKFMDFLSERVLDKKLSNEEKELIYSYAGGKPVDIIYVIQKLKDKNLKEVLNYLLNDVKQKLKYFLEDVKEEDEKLYKEVLEALKIFKESYEVEDITIDKKVREFLVKGNILFLDPIEGILRPQSFLVWNAIKILI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAH4</accession>
    <name>F6BAH4_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="3">PilT protein domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_1452</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96987.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1452"/>
    <dbReference type="EnsemblBacteria" id="AEF96987">
        <property type="protein sequence ID" value="AEF96987"/>
        <property type="gene ID" value="Metig_1452"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_1452"/>
    <dbReference type="HOGENOM" id="CLU_023387_0_0_2"/>
    <dbReference type="OMA" id="FADMRMA"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004088">
        <property type="entry name" value="KH_dom_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009019">
        <property type="entry name" value="KH_sf_prok-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002716">
        <property type="entry name" value="PIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001482">
        <property type="entry name" value="T2SS/T4SS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00013">
        <property type="entry name" value="KH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01850">
        <property type="entry name" value="PIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00437">
        <property type="entry name" value="T2SSE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00670">
        <property type="entry name" value="PINc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54814">
        <property type="entry name" value="SSF54814"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="SSF88723"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PINc" evidence="2">
        <location>
            <begin position="40"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="domain" description="KH" evidence="1">
        <location>
            <begin position="522"/>
            <end position="587"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF96987.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="653" mass="74501" checksum="681E70820D6C13E6" modified="2011-07-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAH9</accession>
    <name>F6BAH9_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 11">Homoserine kinase</fullName>
            <shortName evidence="11">HK</shortName>
            <shortName evidence="11">HSK</shortName>
            <ecNumber evidence="3 11">2.7.1.39</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">thrB</name>
        <name evidence="14" type="ordered locus">Metig_1457</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes the ATP-dependent phosphorylation of L-homoserine to L-homoserine phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>ATP + L-homoserine = ADP + H(+) + O-phospho-L-homoserine</text>
            <dbReference type="Rhea" id="RHEA:13985"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57476"/>
            <dbReference type="ChEBI" id="CHEBI:57590"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.39"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 11">Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 4/5.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the GHMP kinase family. Homoserine kinase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.39" evidence="3 11"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96992.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1457"/>
    <dbReference type="EnsemblBacteria" id="AEF96992">
        <property type="protein sequence ID" value="AEF96992"/>
        <property type="gene ID" value="Metig_1457"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_1457"/>
    <dbReference type="HOGENOM" id="CLU_041243_1_1_2"/>
    <dbReference type="OMA" id="CANRIPH"/>
    <dbReference type="UniPathway" id="UPA00050">
        <property type="reaction ID" value="UER00064"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004413">
        <property type="term" value="F:homoserine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009088">
        <property type="term" value="P:threonine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.890">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00384">
        <property type="entry name" value="Homoser_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013750">
        <property type="entry name" value="GHMP_kinase_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036554">
        <property type="entry name" value="GHMP_kinase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006204">
        <property type="entry name" value="GHMP_kinase_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006203">
        <property type="entry name" value="GHMP_knse_ATP-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000870">
        <property type="entry name" value="Homoserine_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08544">
        <property type="entry name" value="GHMP_kinases_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00288">
        <property type="entry name" value="GHMP_kinases_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000676">
        <property type="entry name" value="Homoser_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55060">
        <property type="entry name" value="SSF55060"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00191">
        <property type="entry name" value="thrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00627">
        <property type="entry name" value="GHMP_KINASES_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="10 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 11 14" id="KW-0418">Kinase</keyword>
    <keyword evidence="8 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 11" id="KW-0791">Threonine biosynthesis</keyword>
    <keyword evidence="6 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GHMP_kinases_N" evidence="12">
        <location>
            <begin position="86"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="domain" description="GHMP_kinases_C" evidence="13">
        <location>
            <begin position="214"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="94"/>
            <end position="104"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00017858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF08544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="AEF96992.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="306" mass="33382" checksum="27423427F1915290" modified="2011-07-27" version="1">MGDKMRKVTVRSPATSANLGPGFDVFGLCLDEPYDIVSVEKMEENGIKIVVEGEKSEEIPTEPDKNTAGVVAKAMMKDFNIKEGIKIHITKGIKPGSGLGSSSASSAGVAVAINELFNLNLPKLKLVEYAALGEGVAAGSPHADNVAPAIFGGFTMVTNYNPLEILHIPVEMNVLIALPDIQISTKEAREILPKKIEMEDMINNVGKACGMVYSLFRNDIELFGKYMMVDKVVEPRRAKLIPKYEEVKEKVKDKVYGITISGAGPAIISIPKKEHLLEVKELFEEVWGRVYYTKVGNGVSVLEKDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAI2</accession>
    <name>F6BAI2_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1460</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96995.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799591.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1460"/>
    <dbReference type="EnsemblBacteria" id="AEF96995">
        <property type="protein sequence ID" value="AEF96995"/>
        <property type="gene ID" value="Metig_1460"/>
    </dbReference>
    <dbReference type="GeneID" id="10644333"/>
    <dbReference type="KEGG" id="mig:Metig_1460"/>
    <dbReference type="HOGENOM" id="CLU_036466_0_0_2"/>
    <dbReference type="OMA" id="DSWSNCN"/>
    <dbReference type="OrthoDB" id="20421at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="498"/>
            <end position="519"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96995.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="524" mass="58772" checksum="B925AB07B174B6C7" modified="2011-07-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAK4</accession>
    <name>F6BAK4_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 8 13">Glutamyl-tRNA reductase</fullName>
            <shortName evidence="8">GluTR</shortName>
            <ecNumber evidence="4 8 13">1.2.1.70</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">hemA</name>
        <name evidence="17" type="ordered locus">Metig_1483</name>
    </gene>
    <organism evidence="18">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the NADPH-dependent reduction of glutamyl-tRNA(Glu) to glutamate 1-semialdehyde (GSA).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8 13">
            <text>(S)-4-amino-5-oxopentanoate + NADP(+) + tRNA(Glu) = H(+) + L-glutamyl-tRNA(Glu) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:12344"/>
            <dbReference type="Rhea" id="RHEA-COMP:9663"/>
            <dbReference type="Rhea" id="RHEA-COMP:9680"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57501"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78520"/>
            <dbReference type="EC" id="1.2.1.70"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 8 13">Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; 5-aminolevulinate from L-glutamyl-tRNA(Glu): step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer.</text>
    </comment>
    <comment type="domain">
        <text evidence="8">Possesses an unusual extended V-shaped dimeric structure with each monomer consisting of three distinct domains arranged along a curved 'spinal' alpha-helix. The N-terminal catalytic domain specifically recognizes the glutamate moiety of the substrate. The second domain is the NADPH-binding domain, and the third C-terminal domain is responsible for dimerization.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="8">During catalysis, the active site Cys acts as a nucleophile attacking the alpha-carbonyl group of tRNA-bound glutamate with the formation of a thioester intermediate between enzyme and glutamate, and the concomitant release of tRNA(Glu). The thioester intermediate is finally reduced by direct hydride transfer from NADPH, to form the product GSA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 8 13">Belongs to the glutamyl-tRNA reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.70" evidence="4 8 13"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97017.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799612.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1483"/>
    <dbReference type="EnsemblBacteria" id="AEF97017">
        <property type="protein sequence ID" value="AEF97017"/>
        <property type="gene ID" value="Metig_1483"/>
    </dbReference>
    <dbReference type="GeneID" id="10644356"/>
    <dbReference type="KEGG" id="mig:Metig_1483"/>
    <dbReference type="HOGENOM" id="CLU_035113_0_0_2"/>
    <dbReference type="OMA" id="FAFKCAA"/>
    <dbReference type="OrthoDB" id="48038at2157"/>
    <dbReference type="UniPathway" id="UPA00251">
        <property type="reaction ID" value="UER00316"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008883">
        <property type="term" value="F:glutamyl-tRNA reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006782">
        <property type="term" value="P:protoporphyrinogen IX biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00087">
        <property type="entry name" value="Glu_tRNA_reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000343">
        <property type="entry name" value="4pyrrol_synth_GluRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015896">
        <property type="entry name" value="4pyrrol_synth_GluRdtase_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015895">
        <property type="entry name" value="4pyrrol_synth_GluRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018214">
        <property type="entry name" value="GluRdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036453">
        <property type="entry name" value="GluRdtase_dimer_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036343">
        <property type="entry name" value="GluRdtase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006151">
        <property type="entry name" value="Shikm_DH/Glu-tRNA_Rdtase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00745">
        <property type="entry name" value="GlutR_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05201">
        <property type="entry name" value="GlutR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01488">
        <property type="entry name" value="Shikimate_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000445">
        <property type="entry name" value="4pyrrol_synth_GluRdtase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69075">
        <property type="entry name" value="SSF69075"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69742">
        <property type="entry name" value="SSF69742"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01035">
        <property type="entry name" value="hemA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00747">
        <property type="entry name" value="GLUTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8 11" id="KW-0521">NADP</keyword>
    <keyword evidence="6 8 13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7 8 13" id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GlutR_N" evidence="16">
        <location>
            <begin position="6"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="domain" description="Shikimate_DH" evidence="15">
        <location>
            <begin position="144"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="domain" description="GlutR_dimer" evidence="14">
        <location>
            <begin position="290"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="8 10">
        <location>
            <begin position="38"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="8 10">
        <location>
            <begin position="87"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="8 9">
        <location>
            <position position="39"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8 10">
        <location>
            <position position="82"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8 10">
        <location>
            <position position="93"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8 11">
        <location>
            <begin position="161"/>
            <end position="166"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="site" description="Important for activity" evidence="8 12">
        <location>
            <position position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000445-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000445-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR000445-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR000445-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF01488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF05201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="AEF97017.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="394" mass="45222" checksum="CEC0C7E50989218C" modified="2011-07-27" version="1">MILLKADYKKYPTSKLEKLRMDEDEFYEKFDGCVLLQTCNRVEIYFDVDNLDEIDIDVDKFDILEGDEAVLHLLRLASGLESMIVGEDQILGQIKKSYLKAKELKKTTKFLDTIFLKAIHVGQRVRSETKINEGGVSIGSAAVELAEKIFGLKNKNVLLIGAGEIGTLVAKALKERHIKAIIKAIIIANRTYERAERLAKELSGIAIHFDKLKEALKYADIVISATGAPHQILTKEDLVDVGETIIIDIANPRDVDDSVRELPHIKLFTIDDLKLISEENLKKRKEEIPKVEKIIMEEFENLKRQIKKLEIQDFIKNFTTYIESIRRKEVEKAVNMLKNKNKSPEEILENFSKAFSKKIIYDFVNVIEERHLHGKDLFVEEVIKEICEKTKYSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAK6</accession>
    <name>F6BAK6_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">3-hydroxy-3-methylglutaryl coenzyme A reductase</fullName>
            <shortName evidence="5">HMG-CoA reductase</shortName>
            <ecNumber evidence="5">1.1.1.34</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Metig_1485</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 5">
            <text>(R)-mevalonate + CoA + 2 NADP(+) = (3S)-hydroxy-3-methylglutaryl-CoA + 2 H(+) + 2 NADPH</text>
            <dbReference type="Rhea" id="RHEA:15989"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:36464"/>
            <dbReference type="ChEBI" id="CHEBI:43074"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.34"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="5">Metabolic intermediate biosynthesis; (R)-mevalonate biosynthesis; (R)-mevalonate from acetyl-CoA: step 3/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the HMG-CoA reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.34" evidence="5"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97019.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799614.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1485"/>
    <dbReference type="EnsemblBacteria" id="AEF97019">
        <property type="protein sequence ID" value="AEF97019"/>
        <property type="gene ID" value="Metig_1485"/>
    </dbReference>
    <dbReference type="GeneID" id="10644358"/>
    <dbReference type="KEGG" id="mig:Metig_1485"/>
    <dbReference type="HOGENOM" id="CLU_001734_2_2_2"/>
    <dbReference type="OMA" id="DDKMTRA"/>
    <dbReference type="OrthoDB" id="26445at2157"/>
    <dbReference type="UniPathway" id="UPA00058">
        <property type="reaction ID" value="UER00103"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004420">
        <property type="term" value="F:hydroxymethylglutaryl-CoA reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015936">
        <property type="term" value="P:coenzyme A metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008299">
        <property type="term" value="P:isoprenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00643">
        <property type="entry name" value="HMG-CoA_reductase_classI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3270.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.770.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002202">
        <property type="entry name" value="HMG_CoA_Rdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023074">
        <property type="entry name" value="HMG_CoA_Rdtase_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023076">
        <property type="entry name" value="HMG_CoA_Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004554">
        <property type="entry name" value="HMG_CoA_Rdtase_eu_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023282">
        <property type="entry name" value="HMG_CoA_Rdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009023">
        <property type="entry name" value="HMG_CoA_Rdtase_NAD(P)-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009029">
        <property type="entry name" value="HMG_CoA_Rdtase_sub-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10572">
        <property type="entry name" value="PTHR10572"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00368">
        <property type="entry name" value="HMG-CoA_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00071">
        <property type="entry name" value="HMGCOARDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55035">
        <property type="entry name" value="SSF55035"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56542">
        <property type="entry name" value="SSF56542"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00533">
        <property type="entry name" value="HMG_CoA_R_NADP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00066">
        <property type="entry name" value="HMG_COA_REDUCTASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00318">
        <property type="entry name" value="HMG_COA_REDUCTASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50065">
        <property type="entry name" value="HMG_COA_REDUCTASE_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0521">NADP</keyword>
    <keyword evidence="4 5 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU361219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEF97019.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="406" mass="44046" checksum="457FB2194DEA657C" modified="2011-07-27" version="1">MPKNNDEIVEKMIKGEIKPYQLESMFDAKKATEIRRRFIEKVTNTTFGYIDKYSIDEEMAMKKNIENMIGAIQIPLGFAGPLKINGEYAKGEFYIPLATTEGALVASVNRGCSVITKCGGATVRVIADKMTRAPVIKTNSVVDAIRLRDWIKENFEKIKEVAESTTRHGKLIDINPILIVGRYVYPRFVYKTGDAMGMNMVTIATEKACNFIEEELKKEGITVHTVALSGNACVDKKPSGINLIEGRGKSIVAEVFLKEGYVQKYLKTTSKAIEQVNMYKNLIGSAISNSMGFNAHYANIIGAIFLATGQDEAHIVEGSLGITVAEATEGGLYFSVTLPDVPIGTVGGGTRVETQRECLEMLGCYGSDKALKFAEIVGGAVLAGELSLLGALAAGHLAKAHAELGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-06-02" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAL6</accession>
    <name>F6BAL6_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1495</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97029.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF97029">
        <property type="protein sequence ID" value="AEF97029"/>
        <property type="gene ID" value="Metig_1495"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_1495"/>
    <dbReference type="HOGENOM" id="CLU_202284_0_0_2"/>
    <dbReference type="OMA" id="HCLNCGI"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019216">
        <property type="entry name" value="DUF2116_Zn-ribbon"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09889">
        <property type="entry name" value="DUF2116"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004990">
        <property type="entry name" value="UCP004990"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="43"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF97029.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="62" mass="7308" checksum="E5AB3C2CB53F8FC9" modified="2011-07-27" version="1">MIPPHRHCLNCGISIPPEESFCSQKCKEEFIAKRKKMMRSQQIFFIAMLIIIFAYIAMVLFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAR7</accession>
    <name>F6BAR7_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Molybdenum ABC transporter, periplasmic molybdate-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0325</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95881.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798490.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0325"/>
    <dbReference type="EnsemblBacteria" id="AEF95881">
        <property type="protein sequence ID" value="AEF95881"/>
        <property type="gene ID" value="Metig_0325"/>
    </dbReference>
    <dbReference type="GeneID" id="10643161"/>
    <dbReference type="KEGG" id="mig:Metig_0325"/>
    <dbReference type="HOGENOM" id="CLU_1048814_0_0_2"/>
    <dbReference type="OMA" id="VENPKPY"/>
    <dbReference type="OrthoDB" id="63612at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015689">
        <property type="term" value="P:molybdate ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005950">
        <property type="entry name" value="ModA"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01256">
        <property type="entry name" value="modA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF95881.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="269" mass="29741" checksum="FEACA616EF9AB353" modified="2011-07-27" version="1">MKKSVVFLIFAIVVLSGCTSNQPTTKIEKFEDLGNDGVTISIGNPEHVPAGRYAMEVLDNLRKSNPKLTDKIIKNIVSKESNVRAVLDKVVSKEVDAGFVYRTDAYMEKDKVKIIKIPKEINVVPEYPIAVLKDSDDKERAEEFVKFVLSKEGQEILEKYGFIPIKNPQSYEPKKIGGEIIVYAAASLTDAFKEISNEFEKKTGCKVKLLFASSGSLRQRIEGGAVGGESGADVFASASLKHMNILKKEGFVDNYSIFAKNELVVITPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAT7</accession>
    <name>F6BAT7_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0284 protein Metig_0345</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0345</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0284 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95901.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798510.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0345"/>
    <dbReference type="EnsemblBacteria" id="AEF95901">
        <property type="protein sequence ID" value="AEF95901"/>
        <property type="gene ID" value="Metig_0345"/>
    </dbReference>
    <dbReference type="GeneID" id="10643182"/>
    <dbReference type="KEGG" id="mig:Metig_0345"/>
    <dbReference type="HOGENOM" id="CLU_053134_0_0_2"/>
    <dbReference type="OMA" id="TRWVAED"/>
    <dbReference type="OrthoDB" id="41909at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008939">
        <property type="term" value="F:nicotinate-nucleotide-dimethylbenzimidazole phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02439">
        <property type="entry name" value="DMB-PRT_CobT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01086">
        <property type="entry name" value="UPF0284"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003200">
        <property type="entry name" value="Nict_dMeBzImd_PRibTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002805">
        <property type="entry name" value="Nict_dMeBzImd_PRibTrfase_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036087">
        <property type="entry name" value="Nict_dMeBzImd_PRibTrfase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38811">
        <property type="entry name" value="PTHR38811"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02277">
        <property type="entry name" value="DBI_PRT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52733">
        <property type="entry name" value="SSF52733"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00303">
        <property type="entry name" value="TIGR00303"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF95901.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="360" mass="38505" checksum="A66BB5D6456F6563" modified="2011-07-27" version="1">MSLITINENGFLDEIKGKKGLFSCVISSIETTKYVPISGVHRDVIAYTPAADMELVVLGKSICLPTPPIDATGCPSPATITRACVDLKNIPALTIDAGCFVKPNVPYIKVNENPTGDIRKGKAMKNSKELFEKGYTLGKNLIYDCLIVGESVPGGTTTALGVLIGLGYDAEGKVSSGSVNNPHELKMEVVRSGLKSAKIEIGKSSVFDVLNAVGDTMMPTVAGMAIAAVENKKPVILAGGTQMAAVLAVIKEINKDAIKSGLISIGTTEFVLNDKNADLKGIVEQIGDVPILASKFYYEKAKIDGLRAYCRGSVKEGVGAGGIAAYAYINGLKPDDVRRYVENNYYRWYKNIINMEINKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAT9</accession>
    <name>F6BAT9_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">5-oxoprolinase (ATP-hydrolyzing)</fullName>
            <ecNumber evidence="2">3.5.2.9</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0347</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.5.2.9" evidence="2"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95903.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798512.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0347"/>
    <dbReference type="EnsemblBacteria" id="AEF95903">
        <property type="protein sequence ID" value="AEF95903"/>
        <property type="gene ID" value="Metig_0347"/>
    </dbReference>
    <dbReference type="GeneID" id="10643184"/>
    <dbReference type="KEGG" id="mig:Metig_0347"/>
    <dbReference type="HOGENOM" id="CLU_020413_0_0_2"/>
    <dbReference type="OMA" id="QVGMTNT"/>
    <dbReference type="OrthoDB" id="4422at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017168">
        <property type="term" value="F:5-oxoprolinase (ATP-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003692">
        <property type="entry name" value="Hydantoinase_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045079">
        <property type="entry name" value="Oxoprolinase_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11365">
        <property type="entry name" value="PTHR11365"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02538">
        <property type="entry name" value="Hydantoinase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Hydantoinase_B" evidence="1">
        <location>
            <begin position="2"/>
            <end position="513"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF95903.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="558" mass="61379" checksum="9A42EF52D2FBF063" modified="2011-07-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAX5</accession>
    <name>F6BAX5_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glycosyl transferase family 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1528</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97062.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799656.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1528"/>
    <dbReference type="EnsemblBacteria" id="AEF97062">
        <property type="protein sequence ID" value="AEF97062"/>
        <property type="gene ID" value="Metig_1528"/>
    </dbReference>
    <dbReference type="GeneID" id="10644401"/>
    <dbReference type="KEGG" id="mig:Metig_1528"/>
    <dbReference type="HOGENOM" id="CLU_023845_4_1_2"/>
    <dbReference type="OMA" id="FFCYCED"/>
    <dbReference type="OrthoDB" id="74894at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glyco_trans_2-like" evidence="1">
        <location>
            <begin position="6"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="domain" description="Glyco_trans_2-like" evidence="1">
        <location>
            <begin position="100"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF97062.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="356" mass="41917" checksum="75661F5720A4BFE7" modified="2011-07-27" version="1">MDPKVSIIILNWNGWRDTIECLESLYRINYDNYNVIVIDNNSEDESIEKIKQYCNGEIEVNSKFFKYSKENKPIKVFELTEDKARNGKFLDKEKYEQLNPNRRLILIKNKDNYGFAGGNNVGIKFALNVLNPKYVLLLNNDTVVDKDFLIEMVKVAESDERIGVVGPKVYFYSEPNKIQTVGVSVKKNPFNIVNIWNYNMIGYGEIDNGQYDVIKQIDSLVGCCILVKTKAINEFGLLDEKFFLYHEESDWLFRISKKYLMYCTPLSKIWHKYSASSGGEFSPTVVYYTTRNSVIFAKKHNNFFNYLLFLIWFLTYKHARRFINFVFLKRKPYLLKYYYKGILDGIIGKYGKRDLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAZ4</accession>
    <name>F6BAZ4_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 7">30S ribosomal protein S7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">rps7</name>
        <name evidence="9" type="ordered locus">Metig_1547</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5 7">One of the primary rRNA binding proteins, it binds directly to 16S rRNA where it nucleates assembly of the head domain of the 30S subunit. Is located at the subunit interface close to the decoding center.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 5 7">Part of the 30S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5 6">Belongs to the universal ribosomal protein uS7 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97081.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799675.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1547"/>
    <dbReference type="EnsemblBacteria" id="AEF97081">
        <property type="protein sequence ID" value="AEF97081"/>
        <property type="gene ID" value="Metig_1547"/>
    </dbReference>
    <dbReference type="GeneID" id="10644420"/>
    <dbReference type="KEGG" id="mig:Metig_1547"/>
    <dbReference type="HOGENOM" id="CLU_063975_0_0_2"/>
    <dbReference type="OMA" id="KMNIVER"/>
    <dbReference type="OrthoDB" id="80498at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd14867">
        <property type="entry name" value="uS7_Eukaryote"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.455.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00480_A">
        <property type="entry name" value="Ribosomal_S7_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000235">
        <property type="entry name" value="Ribosomal_S5/S7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005716">
        <property type="entry name" value="Ribosomal_S5/S7_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026018">
        <property type="entry name" value="Ribosomal_S7_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020606">
        <property type="entry name" value="Ribosomal_S7_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023798">
        <property type="entry name" value="Ribosomal_S7_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036823">
        <property type="entry name" value="Ribosomal_S7_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11205">
        <property type="entry name" value="PTHR11205"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00177">
        <property type="entry name" value="Ribosomal_S7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002122">
        <property type="entry name" value="RPS7p_RPS7a_RPS5e_RPS7o"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47973">
        <property type="entry name" value="SSF47973"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01028">
        <property type="entry name" value="uS7_euk_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00052">
        <property type="entry name" value="RIBOSOMAL_S7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 5 6" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="5 7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5 7" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="Ribosomal_S7" evidence="8">
        <location>
            <begin position="45"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003621"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AEF97081.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="190" mass="21629" checksum="C6699137227D60F8" modified="2011-07-27" version="1">MLEEIKIFGKWDPTEVVVRDPSLRRYINLTPIYVPHTAGRFTKKQFDKAKMSIVERLANKIMRKEHNTGKKLKALKIVEEAFEIIEKRTKQNPIQVLVDALENAGPREETTRISYGGITYLQSVDSSPSRRLDIALRNIALGAWQASHKNKKSIAECLAEEIIAAAKGDIQRSFAVRKKEETERVAQSAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAZ6</accession>
    <name>F6BAZ6_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Elongation factor 1-alpha</fullName>
            <shortName evidence="6">EF-1-alpha</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Elongation factor Tu</fullName>
            <shortName evidence="6">EF-Tu</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">tuf</name>
        <name evidence="8" type="ordered locus">Metig_1549</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-Tu/EF-1A subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97083.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799677.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1549"/>
    <dbReference type="EnsemblBacteria" id="AEF97083">
        <property type="protein sequence ID" value="AEF97083"/>
        <property type="gene ID" value="Metig_1549"/>
    </dbReference>
    <dbReference type="GeneID" id="10644422"/>
    <dbReference type="KEGG" id="mig:Metig_1549"/>
    <dbReference type="HOGENOM" id="CLU_007265_3_5_2"/>
    <dbReference type="OMA" id="AIRDMGM"/>
    <dbReference type="OrthoDB" id="13117at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00118_A">
        <property type="entry name" value="EF_Tu_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031157">
        <property type="entry name" value="G_TR_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009001">
        <property type="entry name" value="Transl_elong_EF1A/Init_IF2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004539">
        <property type="entry name" value="Transl_elong_EF1A_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004160">
        <property type="entry name" value="Transl_elong_EFTu/EF1A_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03143">
        <property type="entry name" value="GTP_EFTU_D3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="SSF50447"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50465">
        <property type="entry name" value="SSF50465"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00483">
        <property type="entry name" value="EF-1_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00301">
        <property type="entry name" value="G_TR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 6 8" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="5 6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="4 6" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Tr-type G" evidence="7">
        <location>
            <begin position="5"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="14"/>
            <end position="21"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="91"/>
            <end position="95"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="149"/>
            <end position="152"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEF97083.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="428" mass="46991" checksum="F441DE34ABAEA658" modified="2011-07-27" version="1">MAKQKPVLNVAFIGHVDAGKSTTIGRLLYDSGAIDPQVLEKLKREAAEKGKAGFEFAYVMDGLKEERERGVTIDIAHKKFETPKYEITIVDCPGHRDFIKNMITGASQADAAILVVDVNDHKTGIQPQTREHMFLARTLGINQIAVAINKMDTVNYSQEAYEAMKKMLSEQLLKILGYNPDNIDFIPVASYHGDNVVKKSDKMPWYNGPTLVEALDKFNPPEKPTNLPLRIPIQDVYSITGVGTVPVGRVETGILKPGDKVIFEPAGVQGEVKSIEMHHEQISQAEPGDNIGFNVRGVSKKDIKRGDVCGHPDNPPTVAEEFTAQIVVLQHPTVITVGYTPVFHAHTAQVACTFTEILKKLNPATGEVIEENPDFIKAGDAAIIKVRPTKPMVIENVKEIPQLGRFAIRDMGMTIAAGMAIDVKAKNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-09-29" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>F6BB06</accession>
    <name>F6BB06_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="5">DNA binding domain protein, excisionase family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Metig_1559</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97093.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799687.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF97093">
        <property type="protein sequence ID" value="AEF97093"/>
        <property type="gene ID" value="Metig_1559"/>
    </dbReference>
    <dbReference type="GeneID" id="10644432"/>
    <dbReference type="KEGG" id="mig:Metig_1559"/>
    <dbReference type="HOGENOM" id="CLU_082093_0_1_2"/>
    <dbReference type="OMA" id="RFIRFGY"/>
    <dbReference type="OrthoDB" id="78802at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000150">
        <property type="term" value="F:DNA strand exchange activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03769">
        <property type="entry name" value="SR_IS607_transposase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1390">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009061">
        <property type="entry name" value="DNA-bd_dom_put_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041657">
        <property type="entry name" value="HTH_17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041718">
        <property type="entry name" value="IS607_transposase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006118">
        <property type="entry name" value="Recombinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006119">
        <property type="entry name" value="Resolv_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036162">
        <property type="entry name" value="Resolvase-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010093">
        <property type="entry name" value="SinI_DNA-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12728">
        <property type="entry name" value="HTH_17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00239">
        <property type="entry name" value="Resolvase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00857">
        <property type="entry name" value="Resolvase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46955">
        <property type="entry name" value="SSF46955"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53041">
        <property type="entry name" value="SSF53041"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01764">
        <property type="entry name" value="excise"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00397">
        <property type="entry name" value="RECOMBINASES_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51736">
        <property type="entry name" value="RECOMBINASES_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0229">DNA integration</keyword>
    <keyword evidence="3" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Resolvase/invertase-type recombinase catalytic" evidence="4">
        <location>
            <begin position="62"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEF97093.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="209" mass="24546" checksum="C28D14F5C4BAAFA8" modified="2011-07-27" version="1">MSLERLYTMKEACELLGVHIKTLQRWDREGKIKCVRTVGGKRRVPESEIKRILGIKDKEQRKIIGYARVSSNTQKDDLERQIDAIKSYAKDRDWNIEILKDVGSGLSEKRKNYRKLLKMVINREVEKVIIAYPDRLTRFGFETLKEFFKSYGTEVIVINKKCKAPQEELVEDLITIISYFARKLYGMRSHKYKKLIKTVKEIVGENNAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F6BB07</accession>
    <name>F6BB07_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Transposase, IS605 OrfB family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Metig_1560</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97094.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799688.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1560"/>
    <dbReference type="EnsemblBacteria" id="AEF97094">
        <property type="protein sequence ID" value="AEF97094"/>
        <property type="gene ID" value="Metig_1560"/>
    </dbReference>
    <dbReference type="GeneID" id="10644433"/>
    <dbReference type="KEGG" id="mig:Metig_1560"/>
    <dbReference type="HOGENOM" id="CLU_032903_3_4_2"/>
    <dbReference type="OMA" id="WATPVDF"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032196">
        <property type="term" value="P:transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010095">
        <property type="entry name" value="Transposase_IS605_OrfB_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07282">
        <property type="entry name" value="OrfB_Zn_ribbon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01766">
        <property type="entry name" value="tspaseT_teng_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0815">Transposition</keyword>
    <feature type="domain" description="OrfB_Zn_ribbon" evidence="4">
        <location>
            <begin position="317"/>
            <end position="378"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF07282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEF97094.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="426" mass="50505" checksum="B542E10B0EF83108" modified="2011-07-27" version="1">MQNKNKLPSEIVLTYKIKHNYDLKNLLDGFMSTSQKAVDIIWENINWKEKQVKHRFKSKNKYKYYTTTRLIPEIPKDKDFKRELRNYLLNGWNFASHYVDGAIKVAYSTIESWKSNYLDGKRKRNKPTFKRPFVRVKNTLIKYDKENGTIRITIKARREYLILDIKNEWFFEKIKGFDIGEIILKDSEALITFKKPLNIFDKKVVIGVDSNLKSLDLFHPEEGWIRIDLSELRRIKNVYDVIIDKLKSIYKKAPKRISILLKKYYNRRKNRVEDFINKLTSQLSKLFPDAIFIFEDLDKPNMYKNSNFNRDLDRTSWREIAKKLEYKSIVFYVNPHYTSKTCPICGSKMESQEGQVVKCEKCGVFNRQFVGSFNIFKRGVKLVKKLLGGVGVLPERSDGNLKTVRFLCPWLGWRSMIYSPMNPEES</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>F6BB25</accession>
    <name>F6BB25_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Bifunctional protein GlmU</fullName>
            <ecNumber evidence="7">2.3.1.157</ecNumber>
            <ecNumber evidence="8">2.7.7.23</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">Metig_0354</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>H(+) + N-acetyl-alpha-D-glucosamine 1-phosphate + UTP = diphosphate + UDP-N-acetyl-alpha-D-glucosamine</text>
            <dbReference type="Rhea" id="RHEA:13509"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
            <dbReference type="ChEBI" id="CHEBI:57776"/>
            <dbReference type="EC" id="2.7.7.23"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>acetyl-CoA + alpha-D-glucosamine 1-phosphate = CoA + H(+) + N-acetyl-alpha-D-glucosamine 1-phosphate</text>
            <dbReference type="Rhea" id="RHEA:13725"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:57776"/>
            <dbReference type="ChEBI" id="CHEBI:58516"/>
            <dbReference type="EC" id="2.3.1.157"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Nucleotide-sugar biosynthesis; UDP-N-acetyl-alpha-D-glucosamine biosynthesis; N-acetyl-alpha-D-glucosamine 1-phosphate from alpha-D-glucosamine 6-phosphate (route II): step 2/2.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4">Nucleotide-sugar biosynthesis; UDP-N-acetyl-alpha-D-glucosamine biosynthesis; UDP-N-acetyl-alpha-D-glucosamine from N-acetyl-alpha-D-glucosamine 1-phosphate: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">In the C-terminal section; belongs to the transferase hexapeptide repeat family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">In the N-terminal section; belongs to the N-acetylglucosamine-1-phosphate uridyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.157" evidence="7"/>
    <dbReference type="EC" id="2.7.7.23" evidence="8"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95910.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0354"/>
    <dbReference type="EnsemblBacteria" id="AEF95910">
        <property type="protein sequence ID" value="AEF95910"/>
        <property type="gene ID" value="Metig_0354"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0354"/>
    <dbReference type="HOGENOM" id="CLU_029499_0_1_2"/>
    <dbReference type="OMA" id="TAIVEHK"/>
    <dbReference type="UniPathway" id="UPA00113">
        <property type="reaction ID" value="UER00532"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019134">
        <property type="term" value="F:glucosamine-1-phosphate N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003977">
        <property type="term" value="F:UDP-N-acetylglucosamine diphosphorylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006048">
        <property type="term" value="P:UDP-N-acetylglucosamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023915">
        <property type="entry name" value="Bifunctiontional_GlmU_arc-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001451">
        <property type="entry name" value="Hexapep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005835">
        <property type="entry name" value="NTP_transferase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011004">
        <property type="entry name" value="Trimer_LpxA-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00132">
        <property type="entry name" value="Hexapep"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00483">
        <property type="entry name" value="NTP_transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51161">
        <property type="entry name" value="SSF51161"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03992">
        <property type="entry name" value="Arch_glmU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="NTP_transferase" evidence="10">
        <location>
            <begin position="9"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001851"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00007707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00007947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00012225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00012457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00013414"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AEF95910.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="426" mass="47199" checksum="5082C0A1CC5E602B" modified="2011-07-27" version="1">MIIGEIMDAVILCAGKGERLRPLTENRPKPMIPVAGKPILEHIIEKVENLVDNIYLVVKYKKEVIIEHFKNRPKIKFLEQGEIDGTGQAVLTAKDYVDGDFLVLNGDIIFEDDLEKFLKCKNAMALTEVKNPENFGVVVLDKDNNIIELQEKPENPKSNLINAGIYKFEDEIFEILEDIEPSERGEVELTDAIKELIKDRKIKGIKLDGYWNDIGRPWDILDANKHLLGKIKTSIKGKVEENVVIKGEVIIEEGATIKANSVIEGPAIIKKGAVVGPLAYIRPNTVLMENTFVGNSSEVKGSIIMKNTKIPHLSYVGDSIIGENCNFGCNTITANLRFDDKPVKVNIKGKRIGSVRKLGVIMGDNVKTGIQVSFMPGVKVGSNCWIGANCLIDKDVESNTFVYKKEEKIIKTLNTIGGLSNMGIGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F6BB51</accession>
    <name>F6BB51_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF553 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_0380</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0252 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95936.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798545.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF95936">
        <property type="protein sequence ID" value="AEF95936"/>
        <property type="gene ID" value="Metig_0380"/>
    </dbReference>
    <dbReference type="GeneID" id="10643217"/>
    <dbReference type="KEGG" id="mig:Metig_0380"/>
    <dbReference type="HOGENOM" id="CLU_045499_0_0_2"/>
    <dbReference type="OMA" id="WIENDDI"/>
    <dbReference type="OrthoDB" id="66569at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007562">
        <property type="entry name" value="Transglutaminase-like_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04473">
        <property type="entry name" value="DUF553"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="SSF54001"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF553" evidence="2">
        <location>
            <begin position="94"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF95936.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="356" mass="42096" checksum="87F25CFFCA999E5F" modified="2011-07-27" version="1">MKRKNYKKYKRRKNYKTYKRRKTRKSVLKRIFGKINTFASLICIGIFVGVLGIILIGNHENKIESNNEYYGMNDKNKYVISEITNALNKNELQTISHLASQLKGRDISESIWNIILWEEENIKYDYPGKHRIGPYVKYPSDTLESSKGICIDYAVLTAGLLLDMGYEPYIILIDEKHDSGHAFCAVNISGTLYALDQHAPIYDINTHIHHLMLNHTISPIFEIHYFKLWIENDDIKYKYGKLYYNNPNNVLTRSDKEKISKDIMNYFISKYPNLKPDKTISNMEHEKYLPKGYSSGLYIYMPFDALYPAFGKYYGAWLDRCFTKEDELRTPINFRKYNRIWVKVDNKRIWVYLAKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F6BB86</accession>
    <name>F6BB86_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_0415</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95971.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798580.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0415"/>
    <dbReference type="EnsemblBacteria" id="AEF95971">
        <property type="protein sequence ID" value="AEF95971"/>
        <property type="gene ID" value="Metig_0415"/>
    </dbReference>
    <dbReference type="GeneID" id="10643252"/>
    <dbReference type="KEGG" id="mig:Metig_0415"/>
    <dbReference type="HOGENOM" id="CLU_712928_0_0_2"/>
    <dbReference type="OMA" id="IDVYEYN"/>
    <dbReference type="OrthoDB" id="55695at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019249">
        <property type="entry name" value="DUF2226"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09987">
        <property type="entry name" value="DUF2226"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF95971.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="422" mass="49504" checksum="13C1558A6CCE6A03" modified="2011-07-27" version="1">MIRVVEGKFVGTENDFNKAINTLSQDFKGYLRISVKKNGNFEEGYIFIKNGKIIGYYYTYDKELFGHDAKESIEKLMNSGYLVEIFEYDDEKLNLMQELEPEIFISDPKNIFKEKEEKRDEILNKPISEHKEDVEFLHIALNIPDGKLIKMNVNKDFKNYLKKGYTLVDVFRKVDSEYERGYIIFKDNEPIVGIYECSKGVLLGIDACPYLKKLLDDENAIIDVYEYSPTKVDIILEAYPKALLLKDENEKKKEKYEESKYEKVTKNEEKIDFGNVGETPISSKQNEDRKENINTELENLSREELLKRLNIKPPDENWVENMVRELTFPSESELEELRYKIKERIYNSIKELDGVRDVDVNIRLNCDYDGKIVCEGEVVVKTKKLLGIIKKDINAKEIENKINNTLKAYHYEFDSKIVIKIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>F6BB88</accession>
    <name>F6BB88_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">D-aminoacyl-tRNA deacylase</fullName>
            <ecNumber evidence="4">3.1.1.96</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">dtdA</name>
        <name evidence="5" type="ordered locus">Metig_0417</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">D-aminoacyl-tRNA deacylase with broad substrate specificity. By recycling D-aminoacyl-tRNA to D-amino acids and free tRNA molecules, this enzyme counteracts the toxicity associated with the formation of D-aminoacyl-tRNA entities in vivo.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>a D-aminoacyl-tRNA + H2O = a D-alpha-amino acid + a tRNA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13953"/>
            <dbReference type="Rhea" id="RHEA-COMP:10123"/>
            <dbReference type="Rhea" id="RHEA-COMP:10124"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:59871"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:79333"/>
            <dbReference type="EC" id="3.1.1.96"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>glycyl-tRNA(Ala) + H2O = glycine + H(+) + tRNA(Ala)</text>
            <dbReference type="Rhea" id="RHEA:53744"/>
            <dbReference type="Rhea" id="RHEA-COMP:9657"/>
            <dbReference type="Rhea" id="RHEA-COMP:13640"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78522"/>
            <dbReference type="EC" id="3.1.1.96"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="4">Binds 2 Zn(2+) ions per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the DtdA deacylase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.96" evidence="4"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95973.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798582.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0417"/>
    <dbReference type="EnsemblBacteria" id="AEF95973">
        <property type="protein sequence ID" value="AEF95973"/>
        <property type="gene ID" value="Metig_0417"/>
    </dbReference>
    <dbReference type="GeneID" id="10643254"/>
    <dbReference type="KEGG" id="mig:Metig_0417"/>
    <dbReference type="HOGENOM" id="CLU_056464_1_0_2"/>
    <dbReference type="OMA" id="CYEATHH"/>
    <dbReference type="OrthoDB" id="84039at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051499">
        <property type="term" value="F:D-aminoacyl-tRNA deacylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019478">
        <property type="term" value="P:D-amino acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00562">
        <property type="entry name" value="Deacylase_DtdA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018033">
        <property type="entry name" value="Deacylase_DtdA_archaea"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007508">
        <property type="entry name" value="DtdA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34667">
        <property type="entry name" value="PTHR34667"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04414">
        <property type="entry name" value="tRNA_deacylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016210">
        <property type="entry name" value="UCP016210"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEF95973.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="253" mass="28906" checksum="C226263A0D7CDCCB" modified="2011-07-27" version="1">MEYLLISSKKDLASQNIKNCIENYGFDVFEIDKELIQITQDDLPKVEAYIFLSKHRSESGKPTLTVHTPGNLTEDNSFGGNKEEICPCDPILNTLLLRNIFKYNNQYKEKIGIFDVSFEVVHHGPSDLNAPAVFVEIGSSEREWVIKEAGEIIAKSIVDTIDQIKNTEYAEGDKIIGFGGGHYAPRFTNLALNGYYVGYIVPKYASVSDRVLEQMINKQEFDYAAFDWKGLRGDDKRRYIKFLEERGVIWKKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>F6BB99</accession>
    <name>F6BB99_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Redox-active disulfide protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metig_0428</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glutaredoxin family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95984.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798593.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0428"/>
    <dbReference type="EnsemblBacteria" id="AEF95984">
        <property type="protein sequence ID" value="AEF95984"/>
        <property type="gene ID" value="Metig_0428"/>
    </dbReference>
    <dbReference type="GeneID" id="10643265"/>
    <dbReference type="KEGG" id="mig:Metig_0428"/>
    <dbReference type="HOGENOM" id="CLU_090389_20_2_2"/>
    <dbReference type="OMA" id="CDDIEVK"/>
    <dbReference type="OrthoDB" id="110723at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004362">
        <property type="term" value="F:glutathione-disulfide reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045454">
        <property type="term" value="P:cell redox homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011767">
        <property type="entry name" value="GLR_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004502">
        <property type="entry name" value="Thio_glut"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012336">
        <property type="entry name" value="Thioredoxin-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13192">
        <property type="entry name" value="Thioredoxin_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00411">
        <property type="entry name" value="redox_disulf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00195">
        <property type="entry name" value="GLUTAREDOXIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0249">Electron transport</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Thioredoxin" evidence="3">
        <location>
            <begin position="1"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEF95984.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="85" mass="9406" checksum="1FE76B75831730DE" modified="2011-07-27" version="1">MLIEVFTSPMCPHCPAAKKVVEDVVGKLSCDDIEVKYIDVTEDPGSVDKYSIMAVPTIVVDGEVAFVGAPSKEQFENYLREKLNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-09-29" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBC3</accession>
    <name>F6BBC3_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="3">DNA polymerase beta domain protein region</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_1597</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97130.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799723.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF97130">
        <property type="protein sequence ID" value="AEF97130"/>
        <property type="gene ID" value="Metig_1597"/>
    </dbReference>
    <dbReference type="GeneID" id="10644470"/>
    <dbReference type="KEGG" id="mig:Metig_1597"/>
    <dbReference type="HOGENOM" id="CLU_130257_1_1_2"/>
    <dbReference type="OMA" id="WANVIDE"/>
    <dbReference type="OrthoDB" id="86612at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041633">
        <property type="entry name" value="Polbeta"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18765">
        <property type="entry name" value="Polbeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="SSF81301"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Polbeta" evidence="2">
        <location>
            <begin position="10"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF18765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF97130.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="130" mass="15300" checksum="9F0063D0B28A1382" modified="2011-07-27" version="1">MLVSKEKIKKKIKEILAEKDEVIFAYLHGSFNEIYFRDVDIAVYVDENKVGDFLDYELKISAEIEKTVRLPVDVKVLNSAPLSFKYRAIKGELLISKNEEVRLRFIERVLMEYLDFKPIEEKIIEEILTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBC7</accession>
    <name>F6BBC7_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Metig_1601</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97134.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1601"/>
    <dbReference type="EnsemblBacteria" id="AEF97134">
        <property type="protein sequence ID" value="AEF97134"/>
        <property type="gene ID" value="Metig_1601"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_1601"/>
    <dbReference type="HOGENOM" id="CLU_646573_0_0_2"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000030">
        <property type="term" value="F:mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007873">
        <property type="entry name" value="Glycosyltransferase_ALG3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05208">
        <property type="entry name" value="ALG3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="39"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="63"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="94"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="178"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="228"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="259"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="297"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="320"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="351"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="394"/>
            <end position="412"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEF97134.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="424" mass="48866" checksum="4998CB260285F85C" modified="2011-07-27" version="1">MVKMGKKFLVGITSIITKLKGKQSRMRNKESSNRNLERIFKFFLAILLFKYSLEFYWTGLLTYPWDIALMMSMHFIAVLLLIDSVLEIFNTSFLSYIGAGLLLSSVLVLFTVYFKEPHFGTDGILFSRYAIDLLFNGENPYSHSMLPAYEKYGLNYRWTTPTLEGGIVDRFSYPPLGFLIFIPLFLLGIPDLNATTILFFVLTLLFLCYEARRLWLLPVIVMFLDPNLFLFSVGGVYDIIWVFFLLLAMKFFYEKQRVLSAVFLGLAISVKQTPALTIPFILIYLLKASGMKDTFKYALTVGATFIIISLPFLLWDPKSYLAGVLAPLSGIMHGMGLASLVYFGHINLHPAFFRVAVSVILATLLALYWINFDKLKHSCWFMPMLILWFHMRSLQNYFISFVPVAMYIFILWQKGKIRGIKHED</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBG5</accession>
    <name>F6BBG5_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Alanine--tRNA ligase</fullName>
            <ecNumber evidence="11">6.1.1.7</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Alanyl-tRNA synthetase</fullName>
            <shortName evidence="11">AlaRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">alaS</name>
        <name evidence="13" type="ordered locus">Metig_1640</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editing domain.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala)</text>
            <dbReference type="Rhea" id="RHEA:12540"/>
            <dbReference type="Rhea" id="RHEA-COMP:9657"/>
            <dbReference type="Rhea" id="RHEA-COMP:9923"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57972"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78497"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="11">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="11">Consists of three domains; the N-terminal catalytic domain, the editing domain and the C-terminal C-Ala domain. The editing domain removes incorrectly charged amino acids, while the C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 11">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.7" evidence="11"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97172.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799764.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1640"/>
    <dbReference type="EnsemblBacteria" id="AEF97172">
        <property type="protein sequence ID" value="AEF97172"/>
        <property type="gene ID" value="Metig_1640"/>
    </dbReference>
    <dbReference type="GeneID" id="10644513"/>
    <dbReference type="KEGG" id="mig:Metig_1640"/>
    <dbReference type="HOGENOM" id="CLU_004485_4_0_2"/>
    <dbReference type="OMA" id="YHHTMFE"/>
    <dbReference type="OrthoDB" id="7896at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004813">
        <property type="term" value="F:alanine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002161">
        <property type="term" value="F:aminoacyl-tRNA editing activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006419">
        <property type="term" value="P:alanyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00036_A">
        <property type="entry name" value="Ala_tRNA_synth_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002318">
        <property type="entry name" value="Ala-tRNA-lgiase_IIc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018162">
        <property type="entry name" value="Ala-tRNA-ligase_IIc_anticod-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018165">
        <property type="entry name" value="Ala-tRNA-synth_IIc_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018164">
        <property type="entry name" value="Ala-tRNA-synth_IIc_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022429">
        <property type="entry name" value="Ala-tRNA_lgiase_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003156">
        <property type="entry name" value="DHHA1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018163">
        <property type="entry name" value="Thr/Ala-tRNA-synth_IIc_edit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012947">
        <property type="entry name" value="tRNA_SAD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02272">
        <property type="entry name" value="DHHA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01411">
        <property type="entry name" value="tRNA-synt_2c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07973">
        <property type="entry name" value="tRNA_SAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00980">
        <property type="entry name" value="TRNASYNTHALA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00863">
        <property type="entry name" value="tRNA_SAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101353">
        <property type="entry name" value="SSF101353"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="SSF50447"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55186">
        <property type="entry name" value="SSF55186"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="SSF55681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03683">
        <property type="entry name" value="A-tRNA_syn_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00344">
        <property type="entry name" value="alaS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50860">
        <property type="entry name" value="AA_TRNA_LIGASE_II_ALA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 11 13" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="7 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 11 13" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9 11" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 11" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2 11" id="KW-0820">tRNA-binding</keyword>
    <keyword evidence="6 11" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="AA_TRNA_LIGASE_II_ALA" evidence="12">
        <location>
            <begin position="59"/>
            <end position="742"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="595"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="599"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="699"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="703"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AEF97172.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="893" mass="102243" checksum="9F0FAADB704DF0AA" modified="2011-07-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBH7</accession>
    <name>F6BBH7_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Site-specific DNA-methyltransferase (cytosine-N(4)-specific)</fullName>
            <ecNumber evidence="3">2.1.1.113</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Metig_1652</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 2'-deoxycytidine in DNA + S-adenosyl-L-methionine = an N(4)-methyl-2'-deoxycytidine in DNA + H(+) + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:16857"/>
            <dbReference type="Rhea" id="RHEA-COMP:11369"/>
            <dbReference type="Rhea" id="RHEA-COMP:13674"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:85452"/>
            <dbReference type="ChEBI" id="CHEBI:137933"/>
            <dbReference type="EC" id="2.1.1.113"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the N(4)/N(6)-methyltransferase family. N(4) subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.113" evidence="3"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97184.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="REBASE" id="36263">
        <property type="entry name" value="M.Mig5ORF1652P"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF97184">
        <property type="protein sequence ID" value="AEF97184"/>
        <property type="gene ID" value="Metig_1652"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_1652"/>
    <dbReference type="HOGENOM" id="CLU_039482_0_0_2"/>
    <dbReference type="OMA" id="KHGKICK"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008170">
        <property type="term" value="F:N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015667">
        <property type="term" value="F:site-specific DNA-methyltransferase (cytosine-N4-specific) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002941">
        <property type="entry name" value="DNA_methylase_N4/N6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017985">
        <property type="entry name" value="MeTrfase_CN4_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01555">
        <property type="entry name" value="N6_N4_Mtase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00093">
        <property type="entry name" value="N4_MTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N6_N4_Mtase" evidence="7">
        <location>
            <begin position="63"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEF97184.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="531" mass="64070" checksum="4ECBC04564B01FEB" modified="2011-07-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBI0</accession>
    <name>F6BBI0_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_0433</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95989.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798598.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0433"/>
    <dbReference type="EnsemblBacteria" id="AEF95989">
        <property type="protein sequence ID" value="AEF95989"/>
        <property type="gene ID" value="Metig_0433"/>
    </dbReference>
    <dbReference type="GeneID" id="10643270"/>
    <dbReference type="KEGG" id="mig:Metig_0433"/>
    <dbReference type="HOGENOM" id="CLU_167520_0_0_2"/>
    <dbReference type="OMA" id="ENDLFGH"/>
    <dbReference type="OrthoDB" id="100749at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027396">
        <property type="entry name" value="DsrEFH-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003787">
        <property type="entry name" value="Sulphur_relay_DsrE/F-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02635">
        <property type="entry name" value="DrsE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75169">
        <property type="entry name" value="SSF75169"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF95989.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="116" mass="13369" checksum="74390083B57F8691" modified="2011-07-27" version="1">MKTAFLVFSYEKNGKPNMPIMFHTLLFANEMKEKGDEVKIILEGEGVNWAKDLLNEDHPLKNHFEKLKDDFVVCEACASMFGVKEKIEGKLKLDNDLFGHVSLKKYLDDGYQIIEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBK7</accession>
    <name>F6BBK7_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Radical SAM domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Metig_0460</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96016.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798625.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0460"/>
    <dbReference type="EnsemblBacteria" id="AEF96016">
        <property type="protein sequence ID" value="AEF96016"/>
        <property type="gene ID" value="Metig_0460"/>
    </dbReference>
    <dbReference type="GeneID" id="10643297"/>
    <dbReference type="KEGG" id="mig:Metig_0460"/>
    <dbReference type="HOGENOM" id="CLU_058377_0_0_2"/>
    <dbReference type="OMA" id="CIYCELG"/>
    <dbReference type="OrthoDB" id="73459at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040084">
        <property type="entry name" value="GTPase_Obg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01083">
        <property type="entry name" value="Uncharacterised_Radical_SAM_Su"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="6">
        <location>
            <begin position="6"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEF96016.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="312" mass="35885" checksum="140311B617158BA5" modified="2011-07-27" version="1">MIVYGPVPSRRLGLSLGIDPIYYTCTFDCIYCQLGRTRYLVSSPKEIPKEILEKFPTDEDIYNEVKKIVDNCRGIDYITFAGSGEPTLSPYLKDAIERLKEFNIPICVITNSSLLKYKKVRNALKETDLVSATLTSTNQETFEKIHRTKIKLKDVVDGLIKFRRVAKETILNIELMVLEGINDDNEVIYKLKEIIDKINPNNIEINTPIRPPCEKYAKKVSYGRLKEIKEILGEKAYIIETCKKKYKESLNSNFLERISSILKIRPCTVEDLANVLGLHVSEVGKYLYALKNSENLECTEIDGKKYYFIKIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBM6</accession>
    <name>F6BBM6_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 4">DNA repair and recombination protein RadB</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">radB</name>
        <name evidence="6" type="ordered locus">Metig_0479</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3 4">Involved in DNA repair and in homologous recombination. May regulate the cleavage reactions of the branch-structured DNA. Has a very weak ATPase activity that is not stimulated by DNA. Binds DNA but does not promote DNA strands exchange.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic RecA-like protein family. RadB subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96035.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798643.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0479"/>
    <dbReference type="EnsemblBacteria" id="AEF96035">
        <property type="protein sequence ID" value="AEF96035"/>
        <property type="gene ID" value="Metig_0479"/>
    </dbReference>
    <dbReference type="GeneID" id="10643316"/>
    <dbReference type="KEGG" id="mig:Metig_0479"/>
    <dbReference type="HOGENOM" id="CLU_041732_2_0_2"/>
    <dbReference type="OMA" id="GNTLEHW"/>
    <dbReference type="OrthoDB" id="93716at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00350">
        <property type="entry name" value="RadB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013632">
        <property type="entry name" value="DNA_recomb/repair_Rad51_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011939">
        <property type="entry name" value="DNA_repair_and_recomb_RadB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020588">
        <property type="entry name" value="RecA_ATP-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08423">
        <property type="entry name" value="Rad51"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003336">
        <property type="entry name" value="RadB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02237">
        <property type="entry name" value="recomb_radB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50162">
        <property type="entry name" value="RECA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0227">DNA damage</keyword>
    <keyword evidence="4" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RECA_2" evidence="5">
        <location>
            <begin position="1"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00018143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00024641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEF96035.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="213" mass="24453" checksum="A2CE3E4F3EEE237C" modified="2011-07-27" version="1">MLKNILKGEIEKKTITQLYGPPGAGKTNICIISSINTIKKGKKVVYIDTEGSLSLERIKQICPNDFEDILKNMIIYEPYDFEEQTEVIEKKVPLLNNVGLIVVDGIASLYRLELSDDVNKNARLNRILGRQILNLLKVAKKNDLAILITNQIRDTLDGFEATGGRILEYWSKTIVRIEKYEQYREAILEKHRYAKEGEKVRFKIVDRGIEIID</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-06-02" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBN2</accession>
    <name>F6BBN2_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0485</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96041.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96041">
        <property type="protein sequence ID" value="AEF96041"/>
        <property type="gene ID" value="Metig_0485"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0485"/>
    <dbReference type="HOGENOM" id="CLU_2766084_0_0_2"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="23"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96041.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="69" mass="7542" checksum="AFE90C1A810CB3E3" modified="2011-07-27" version="1">MMPLMKVDTGDLRRRTLLSVKPMGGAGVPVTGMETDDMFPMNPEGTEADDIQVQRGHLSMIVHVGQTPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-06-02" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBR1</accession>
    <name>F6BBR1_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_1659</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97191.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF97191">
        <property type="protein sequence ID" value="AEF97191"/>
        <property type="gene ID" value="Metig_1659"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_1659"/>
    <dbReference type="HOGENOM" id="CLU_2712840_0_0_2"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF97191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="73" mass="8582" checksum="C40C989ABB012E27" modified="2011-07-27" version="1">MSFRYIYIGLYKDKKNRWVELGVISERSDENLKTVRFLCPRQGQRHSCVADDAQVQYGYLLTNTHIGQTPLLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBX3</accession>
    <name>F6BBX3_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Probable GTP-binding protein EngB</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">engB</name>
        <name evidence="9" type="ordered locus">Metig_1721</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Necessary for normal cell division and for the maintenance of normal septation.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. EngB GTPase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97253.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799842.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1721"/>
    <dbReference type="EnsemblBacteria" id="AEF97253">
        <property type="protein sequence ID" value="AEF97253"/>
        <property type="gene ID" value="Metig_1721"/>
    </dbReference>
    <dbReference type="GeneID" id="10644595"/>
    <dbReference type="KEGG" id="mig:Metig_1721"/>
    <dbReference type="HOGENOM" id="CLU_033732_3_0_2"/>
    <dbReference type="OMA" id="PNHYDWA"/>
    <dbReference type="OrthoDB" id="73254at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000917">
        <property type="term" value="P:division septum assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01876">
        <property type="entry name" value="YihA_EngB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00321">
        <property type="entry name" value="GTPase_EngB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030393">
        <property type="entry name" value="G_ENGB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019987">
        <property type="entry name" value="GTP-bd_ribosome_bio_YsxC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51706">
        <property type="entry name" value="G_ENGB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="7" id="KW-0132">Cell division</keyword>
    <keyword evidence="6 7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0717">Septation</keyword>
    <feature type="domain" description="EngB-type G" evidence="8">
        <location>
            <begin position="22"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AEF97253.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="220" mass="25709" checksum="E365D93DBC68C636" modified="2011-07-27" version="1">MTDFFERYKKIKSVKIKSKKLEKPKVVVAGRSNVGKSTFVRHITGKNVRVGKRPGVTLKINEYDMGNYILVDMPGFGFMHGIDKKVQEKIKDEIVHYIEGKRDEIATAIQIIDAKSFLEIVERWDQRGEIPIDIEMFDFLNELELNPILVVNKMDKIKKTQWDEHLNKIVETLGYKPPWSQWEFVVPAILKEKKGLDEIKHKIIERVNKFKSQKTVDIDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>F6BC24</accession>
    <name>F6BC24_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="8">ABC-type transporter, integral membrane subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Metig_0551</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the binding-protein-dependent transport system permease family. FecCD subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96105.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798712.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0551"/>
    <dbReference type="EnsemblBacteria" id="AEF96105">
        <property type="protein sequence ID" value="AEF96105"/>
        <property type="gene ID" value="Metig_0551"/>
    </dbReference>
    <dbReference type="GeneID" id="10643389"/>
    <dbReference type="KEGG" id="mig:Metig_0551"/>
    <dbReference type="HOGENOM" id="CLU_013016_0_0_2"/>
    <dbReference type="OMA" id="WFAGSFT"/>
    <dbReference type="OrthoDB" id="36067at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3470.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037294">
        <property type="entry name" value="ABC_BtuC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000522">
        <property type="entry name" value="ABC_transptr_permease_BtuC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30472">
        <property type="entry name" value="PTHR30472"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01032">
        <property type="entry name" value="FecCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81345">
        <property type="entry name" value="SSF81345"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="59"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="91"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="119"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="152"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="179"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="205"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="244"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="287"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="321"/>
            <end position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEF96105.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="347" mass="37440" checksum="7A6FDEF6335FCC92" modified="2011-07-27" version="1">MKIRVGLLSIFLLFILFSLLILSITNGTIKIEPKNFYNYVLEGTTGNPIYDKIIEKCRIPRTVGAVFAGMGLAVAGLLMQSLFRNPLADPYLIGVSGGASLGVALYVFTSLLFKFGIPHSIWGFIISAYLGSLLSMFVVLSLAKKVKQITTLLIVGLMIGYISSGLITIVIAFSDFVGVNNDVLASFMMWGYGSLSSLTMKQSLIMGVLVVICCFITYSLSKFLDAYLLGENYAKSVGIDIKKLRILIILISCMITATIVAFAGPIGFIGLVCPITARLLCGTSKHIYVIPNAMLIGAIFLIVADILVRPGIVIPTTSSELPLMCPLAIIGSPIAIIIYIKRKIMGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-06-02" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>F6BC50</accession>
    <name>F6BC50_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0577</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96131.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96131">
        <property type="protein sequence ID" value="AEF96131"/>
        <property type="gene ID" value="Metig_0577"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0577"/>
    <dbReference type="HOGENOM" id="CLU_100473_0_0_2"/>
    <dbReference type="OMA" id="FGIHIIL"/>
    <dbReference type="OrthoDB" id="65276at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038880">
        <property type="entry name" value="MJ0871-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38139">
        <property type="entry name" value="PTHR38139"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="41"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="74"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="100"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="156"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="194"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="231"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="268"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96131.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="292" mass="32631" checksum="A9B6893788477AE3" modified="2011-07-27" version="1">MMVLEIFSKVLPLIFLGVLLANIMCHLNILYKLQKYIKNKYFPILAVYFVSSTSGHFLLNNLCDKGELSKENLMPLYFLGMFSFGIHIILFFAIPMATSLGLYVGGLYVLIKFLVTCMYLLFGILMLRKNNTQINVDFNFKSGGIKAIIRDTLKQYARVLMSFVPSVLIITYLIEHGLLEIVENLADSVLDALGLPSTVLIIMLTGLATISGAIGIASGLLSGNVLTPNEILFSLLVAEFLNKITIYLRKNLPLQLSIFGKIGIKLATLHLLIYEIPLLIVIVVWYLVFIRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-06-02" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>F6BC51</accession>
    <name>F6BC51_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">4-vinyl reductase 4VR</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0578</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96132.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798739.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96132">
        <property type="protein sequence ID" value="AEF96132"/>
        <property type="gene ID" value="Metig_0578"/>
    </dbReference>
    <dbReference type="GeneID" id="10643416"/>
    <dbReference type="KEGG" id="mig:Metig_0578"/>
    <dbReference type="HOGENOM" id="CLU_128702_0_0_2"/>
    <dbReference type="OMA" id="NIGKRIC"/>
    <dbReference type="OrthoDB" id="114615at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019642">
        <property type="entry name" value="DUF2507"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024096">
        <property type="entry name" value="NO_sig/Golgi_transp_ligand-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004096">
        <property type="entry name" value="V4R"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10702">
        <property type="entry name" value="DUF2507"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02830">
        <property type="entry name" value="V4R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00989">
        <property type="entry name" value="V4R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111126">
        <property type="entry name" value="SSF111126"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="V4R" evidence="1">
        <location>
            <begin position="84"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96132.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="148" mass="16330" checksum="687D2F139CC89FF6" modified="2011-07-27" version="1">MAQIPLDILKNTNRPTLGDSINTVLFRIIRFADLEKYLGDGANAVLYASGKKFGESLDLKSVDDVIEFCKDNKIGIVEIVNENPLQVRVYECITCSGLPKMGKTLCHFEGGFIAGCLENIFGKKVQVKETHCAGLGHEYCQFDVKILG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>F6BC59</accession>
    <name>F6BC59_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Probable glucose-6-phosphate isomerase</fullName>
            <shortName evidence="7">GPI</shortName>
            <ecNumber evidence="7">5.3.1.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Phosphoglucose isomerase</fullName>
            <shortName evidence="7">PGI</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Phosphohexose isomerase</fullName>
            <shortName evidence="7">PHI</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">pgi</name>
        <name evidence="8" type="ordered locus">Metig_1733</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the reversible isomerization of glucose-6-phosphate to fructose-6-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 7">
            <text>alpha-D-glucose 6-phosphate = beta-D-fructose 6-phosphate</text>
            <dbReference type="Rhea" id="RHEA:11816"/>
            <dbReference type="ChEBI" id="CHEBI:57634"/>
            <dbReference type="ChEBI" id="CHEBI:58225"/>
            <dbReference type="EC" id="5.3.1.9"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="7">Carbohydrate biosynthesis; gluconeogenesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 7">Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 2/4.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the GPI family.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.9" evidence="7"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97265.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1733"/>
    <dbReference type="EnsemblBacteria" id="AEF97265">
        <property type="protein sequence ID" value="AEF97265"/>
        <property type="gene ID" value="Metig_1733"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_1733"/>
    <dbReference type="HOGENOM" id="CLU_037303_1_0_2"/>
    <dbReference type="OMA" id="CPAYAYG"/>
    <dbReference type="OrthoDB" id="24853at2157"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00181"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00138"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097367">
        <property type="term" value="F:carbohydrate derivative binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004347">
        <property type="term" value="F:glucose-6-phosphate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd05015">
        <property type="entry name" value="SIS_PGI_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05016">
        <property type="entry name" value="SIS_PGI_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00473">
        <property type="entry name" value="G6P_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001672">
        <property type="entry name" value="G6P_Isomerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018189">
        <property type="entry name" value="Phosphoglucose_isomerase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046348">
        <property type="entry name" value="SIS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035476">
        <property type="entry name" value="SIS_PGI_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035482">
        <property type="entry name" value="SIS_PGI_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11469">
        <property type="entry name" value="PTHR11469"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00342">
        <property type="entry name" value="PGI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00662">
        <property type="entry name" value="G6PISOMERASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53697">
        <property type="entry name" value="SSF53697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00174">
        <property type="entry name" value="P_GLUCOSE_ISOMERASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51463">
        <property type="entry name" value="P_GLUCOSE_ISOMERASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 7" id="KW-0312">Gluconeogenesis</keyword>
    <keyword evidence="4 7" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="5 7 8" id="KW-0413">Isomerase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Proton donor" evidence="7">
        <location>
            <position position="272"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="293"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="401"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00029321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEF97265.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="420" mass="47610" checksum="452B0216D1E33A7F" modified="2011-07-27" version="1">MIMQWTDVLGAEGVSTKDIENMGNEIENARMGFMEKIKNGEIGFTNLLNVDLGNYDEIKKYMEEFDYILIIGMGGSILGTEAIHEGINGLYYNEHGFPKVYFLDNSDPEKTCRVLNLIDLEKTLIFVVSKSGNTLETLANFSIIKNILEKKVKNYEKNIIAITNGGYLKKLAEKGGYILFNIPENVSGRYSVFSDVGLAPLSCLGINIKELISGAKIMDKLCKKEDVFENPALMSATIHYLMYKKRKNISVIMPYAERLYKFGMWYRQLWAESLGKDGIGQTPLVSLGAKDQHSLLQLYMDGPKDKIVTFIRVNTFSEDIKTEYGEGNLNMHNLSEIINSEQLATEMSLTNHKVPNVKIILEELNETTLGMLLYMYEMQTAFSGELFGVNSFDQPAVEEEKKLTWRLLEEGNYGNKRISK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>F6BC61</accession>
    <name>F6BC61_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Aminotransferase</fullName>
            <ecNumber evidence="1">2.6.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_1735</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.-" evidence="1"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97267.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799856.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1735"/>
    <dbReference type="EnsemblBacteria" id="AEF97267">
        <property type="protein sequence ID" value="AEF97267"/>
        <property type="gene ID" value="Metig_1735"/>
    </dbReference>
    <dbReference type="GeneID" id="10644609"/>
    <dbReference type="KEGG" id="mig:Metig_1735"/>
    <dbReference type="HOGENOM" id="CLU_017584_4_5_2"/>
    <dbReference type="OMA" id="YPHMPTG"/>
    <dbReference type="OrthoDB" id="32104at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009058">
        <property type="term" value="P:biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004838">
        <property type="entry name" value="NHTrfase_class1_PyrdxlP-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00105">
        <property type="entry name" value="AA_TRANSFER_CLASS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotran_1_2" evidence="2">
        <location>
            <begin position="46"/>
            <end position="369"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF97267.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="418" mass="47845" checksum="1AD1C67B6EA4A7A9" modified="2011-07-27" version="1">MESYIQNLFAERIGGKKFGKEDVIYKFEKIKRAKQEAMKRHPDMELIDMGVGEPDEMADPEVIKVLCEEAKKWENRGYADNGIQELKDAVPPYMEKVYGVKDIDPVNEVIHSMGSKPALAYITSAFINPGDVTLMTVPGYPVTATHTKWYGGEVYNLPLLEENDFLPDLESIPEDIKKRAKILYLNYPNNPTGAQATKKFYKEVVDFAFENEVIVVQDAAYGALVYDGKPLSFLSVKDAKEVGVEIHSFSKAYNMTGWRLAFLVGNELIIKAFATVKDNFDSGQFIPIQKAGIYCLQHPEITERVRQKYERRLRKMVKILNEVGFKARMPGGTFYLYVKSPRRANGIEFETAEDFSQYLIKEKLISTVPWDDAGHYLRLAACFVAKDENGNPTTEEKYEDMVLEEFKRRLEGMEFEFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>F6BC63</accession>
    <name>F6BC63_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Nitrogen regulatory protein P-II</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1737</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the P(II) protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97269.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799858.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1737"/>
    <dbReference type="EnsemblBacteria" id="AEF97269">
        <property type="protein sequence ID" value="AEF97269"/>
        <property type="gene ID" value="Metig_1737"/>
    </dbReference>
    <dbReference type="GeneID" id="10644611"/>
    <dbReference type="KEGG" id="mig:Metig_1737"/>
    <dbReference type="HOGENOM" id="CLU_082268_0_1_2"/>
    <dbReference type="OMA" id="LMIRTIV"/>
    <dbReference type="OrthoDB" id="113533at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030234">
        <property type="term" value="F:enzyme regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006808">
        <property type="term" value="P:regulation of nitrogen utilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002187">
        <property type="entry name" value="N-reg_PII"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011322">
        <property type="entry name" value="N-reg_PII-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015867">
        <property type="entry name" value="N-reg_PII/ATP_PRibTrfase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017918">
        <property type="entry name" value="N-reg_PII_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30115">
        <property type="entry name" value="PTHR30115"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00543">
        <property type="entry name" value="P-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00340">
        <property type="entry name" value="PIIGLNB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00938">
        <property type="entry name" value="P-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54913">
        <property type="entry name" value="SSF54913"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00638">
        <property type="entry name" value="PII_GLNB_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51343">
        <property type="entry name" value="PII_GLNB_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU003936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF97269.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="105" mass="11589" checksum="135360E90130688B" modified="2011-07-27" version="1">MKLIKAIVRPEKVDDIVEALEKAGYPSFTKIDVVGRGKQGGLKVGEIFYDELPKTMLLIGVNDDEVDEVVEIIKTNAYTGNFGDGKVFIQPIEEAYTIRTGEKGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>F6BC88</accession>
    <name>F6BC88_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase</fullName>
            <ecNumber evidence="9">4.1.3.44</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">tRNA wyosine derivatives biosynthesis protein Taw1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">taw1</name>
        <name evidence="11" type="ordered locus">Metig_1762</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Component of the wyosine derivatives biosynthesis pathway that catalyzes the condensation of N-methylguanine with 2 carbon atoms from pyruvate to form the tricyclic 4-demethylwyosine (imG-14) on guanosine-37 of tRNA(Phe).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>N(1)-methylguanosine(37) in tRNA(Phe) + pyruvate + S-adenosyl-L-methionine = 4-demethylwyosine(37) in tRNA(Phe) + 5'-deoxyadenosine + CO2 + H2O + L-methionine</text>
            <dbReference type="Rhea" id="RHEA:36347"/>
            <dbReference type="Rhea" id="RHEA-COMP:10164"/>
            <dbReference type="Rhea" id="RHEA-COMP:10165"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:64315"/>
            <dbReference type="ChEBI" id="CHEBI:73542"/>
            <dbReference type="EC" id="4.1.3.44"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="9">Binds 2 [4Fe-4S] clusters. Binds 1 [4Fe-4S] cluster coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the TYW1 family.</text>
    </comment>
    <dbReference type="EC" id="4.1.3.44" evidence="9"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97294.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799883.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1762"/>
    <dbReference type="EnsemblBacteria" id="AEF97294">
        <property type="protein sequence ID" value="AEF97294"/>
        <property type="gene ID" value="Metig_1762"/>
    </dbReference>
    <dbReference type="GeneID" id="10644636"/>
    <dbReference type="KEGG" id="mig:Metig_1762"/>
    <dbReference type="HOGENOM" id="CLU_007952_3_0_2"/>
    <dbReference type="OMA" id="HRCLQMT"/>
    <dbReference type="OrthoDB" id="36053at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102521">
        <property type="term" value="F:tRNA-4-demethylwyosine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01921">
        <property type="entry name" value="TYW1_archaea"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013917">
        <property type="entry name" value="tRNA_wybutosine-synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034556">
        <property type="entry name" value="tRNA_wybutosine-synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023993">
        <property type="entry name" value="TYW1_archaea"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13930">
        <property type="entry name" value="PTHR13930"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08608">
        <property type="entry name" value="Wyosine_form"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00284">
        <property type="entry name" value="tRNA_wybutosine-synthesizing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03972">
        <property type="entry name" value="rSAM_TYW1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 9" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 9" id="KW-0408">Iron</keyword>
    <keyword evidence="7 9" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="8 9" id="KW-0456">Lyase</keyword>
    <keyword evidence="5 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4 9" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="Radical SAM core" evidence="10">
        <location>
            <begin position="43"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="24"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="37"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="50"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="60"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="64"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="67"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AEF97294.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="310" mass="36658" checksum="272BD901C66BE361" modified="2011-07-27" version="1">MEEIYQILRKQRYQIKNHTAVKLCGWVRKCLLENKRCYKSKFYGIETHRCIQCTPAVVWCQQNCIFCWRVLPSDINMQGSLKEPKWDEPEVVFEQILKMHRTMIMGYKGVIDRVGEEKFEEALNPRHVAISLSGEPTLYPYLDELIKIFHKNGFSTFVVSNGILTDVIEKIEPTQLYVSLDAYDVESYKRICRGKEEYWEKILNTLDILGEKKRTCIRTTVIRHLNDDILKFVDLYERANVNFIEIKSYMNVGYSRRRLKLEDMLKPEEILELAKILEENSCYKFVDKSSDSRVALLVNENKKINPKIDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCA1</accession>
    <name>F6BCA1_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 8">Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B</fullName>
            <shortName evidence="8">Asp/Glu-ADT subunit B</shortName>
            <ecNumber evidence="8">6.3.5.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">gatB</name>
        <name evidence="10" type="ordered locus">Metig_1775</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp-tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction takes place in the presence of glutamine and ATP through an activated phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA(Gln).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>ATP + H2O + L-aspartyl-tRNA(Asn) + L-glutamine = ADP + 2 H(+) + L-asparaginyl-tRNA(Asn) + L-glutamate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:14513"/>
            <dbReference type="Rhea" id="RHEA-COMP:9674"/>
            <dbReference type="Rhea" id="RHEA-COMP:9677"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:78515"/>
            <dbReference type="ChEBI" id="CHEBI:78516"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>ATP + H2O + L-glutamine + L-glutamyl-tRNA(Gln) = ADP + H(+) + L-glutamate + L-glutaminyl-tRNA(Gln) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:17521"/>
            <dbReference type="Rhea" id="RHEA-COMP:9681"/>
            <dbReference type="Rhea" id="RHEA-COMP:9684"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:78520"/>
            <dbReference type="ChEBI" id="CHEBI:78521"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="8">Heterotrimer of A, B and C subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the GatB/GatE family. GatB subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.-" evidence="8"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97307.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799896.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1775"/>
    <dbReference type="EnsemblBacteria" id="AEF97307">
        <property type="protein sequence ID" value="AEF97307"/>
        <property type="gene ID" value="Metig_1775"/>
    </dbReference>
    <dbReference type="GeneID" id="10644649"/>
    <dbReference type="KEGG" id="mig:Metig_1775"/>
    <dbReference type="HOGENOM" id="CLU_019240_0_0_2"/>
    <dbReference type="OMA" id="ARKWWMG"/>
    <dbReference type="OrthoDB" id="21200at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050566">
        <property type="term" value="F:asparaginyl-tRNA synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050567">
        <property type="term" value="F:glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.410">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.380">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00121">
        <property type="entry name" value="GatB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004413">
        <property type="entry name" value="Apn/Gln-ADT_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017959">
        <property type="entry name" value="Asn/Gln-tRNA_amidoTrfase_suB/E"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006075">
        <property type="entry name" value="Asn/Gln-tRNA_Trfase_suB/E_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018027">
        <property type="entry name" value="Asn/Gln_amidotransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003789">
        <property type="entry name" value="Asn/Gln_tRNA_amidoTrase-B-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042114">
        <property type="entry name" value="GatB_C_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023168">
        <property type="entry name" value="GatB_Yqey_C_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017958">
        <property type="entry name" value="Gln-tRNA_amidoTrfase_suB_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014746">
        <property type="entry name" value="Gln_synth/guanido_kin_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11659">
        <property type="entry name" value="PTHR11659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02934">
        <property type="entry name" value="GatB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02637">
        <property type="entry name" value="GatB_Yqey"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00845">
        <property type="entry name" value="GatB_Yqey"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55931">
        <property type="entry name" value="SSF55931"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89095">
        <property type="entry name" value="SSF89095"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00133">
        <property type="entry name" value="gatB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01234">
        <property type="entry name" value="GATB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 8" id="KW-0436">Ligase</keyword>
    <keyword evidence="5 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GatB_Yqey" evidence="9">
        <location>
            <begin position="314"/>
            <end position="467"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AEF97307.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="470" mass="53793" checksum="B83D2CC3569B6B5A" modified="2011-07-27" version="1">MEDDVKMKCGLEIHVQVDTESKLFCKCPTNYKDVPPNTNVCPVCMGLPGAKPMPPNKKAVDVAIMVAKMLGCEIVINKDIYFQRKHYDYPDLPSGYQRTSVPIGVNGNFMGIGITEVHLEEDPGQYKPDLGLVDYNRSGTPLIEIVTEPDIKSPEEAREFLKQLMRLFRYIGCLRGEGSMRADVNISINYKGVQGNRVEVKNINSIKGVYKVLKYELIRQKNIIRRGGTVKRETRAFLESQMITKSMREKETAEDYRYIPDPDIQPIVIDEKWVKEVEEKMPETPLEKEKRFIREYGIREDDAKILVSDLELADAFEKVVAGLGKEKENINLAVTWIRNELKRVLQYNKIDFFESNIKVEHLIELIKLIKDKVISQKIGKKVIELLVEYRGEKSPKQIIDELGLTVITDEDTLEKACDEAIKNNPKAVEDYLNGNKPALNFLMGQVMKLTRGRADPKKVVEILKKKLDNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCB4</accession>
    <name>F6BCB4_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Sua5/YciO/YrdC/YwlC family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1788</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97320.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799909.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1788"/>
    <dbReference type="EnsemblBacteria" id="AEF97320">
        <property type="protein sequence ID" value="AEF97320"/>
        <property type="gene ID" value="Metig_1788"/>
    </dbReference>
    <dbReference type="GeneID" id="10644665"/>
    <dbReference type="KEGG" id="mig:Metig_1788"/>
    <dbReference type="HOGENOM" id="CLU_031397_3_2_2"/>
    <dbReference type="OMA" id="MLYPTDT"/>
    <dbReference type="OrthoDB" id="52427at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003725">
        <property type="term" value="F:double-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017945">
        <property type="entry name" value="DHBP_synth_RibB-like_a/b_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006070">
        <property type="entry name" value="YrdC-like_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01300">
        <property type="entry name" value="Sua5_yciO_yrdC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55821">
        <property type="entry name" value="SSF55821"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00057">
        <property type="entry name" value="TIGR00057"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51163">
        <property type="entry name" value="YRDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="YrdC-like" evidence="1">
        <location>
            <begin position="14"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF97320.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="210" mass="23644" checksum="0958ACA327285AA6" modified="2011-07-27" version="1">MIIKINEMNEKEKMEAIKKIQKMILEGKVVICGTDTLYGICVNALDENAVKKVYKIKKRDFGKYISISLKDKEEIEKYAYVNDVARKIIDKFMPGPITIILKKKDVIPDIVAKDYIGIRVPDNDVVRELAIVPLTSTSANISGEKSPTSVDEISEEIKKNVDVIVDTGKCKYSKPSTIIKICESGKIELVREGAIPFKEIVKYCQILSNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCD3</accession>
    <name>F6BCD3_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DNA/RNA-binding protein Alba</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">albA</name>
        <name evidence="9" type="ordered locus">Metig_0590</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Binds double-stranded DNA tightly but without sequence specificity. It is distributed uniformly and abundantly on the chromosome, suggesting a role in chromatin architecture. However, it does not significantly compact DNA. Binds rRNA and mRNA in vivo. May play a role in maintaining the structural and functional stability of RNA, and, perhaps, ribosomes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Chromosome</location>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="7">Acetylated. Deacetylation by the SIR2-homolog deacetylase may regulate its activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the histone-like Alba family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96144.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798751.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0590"/>
    <dbReference type="EnsemblBacteria" id="AEF96144">
        <property type="protein sequence ID" value="AEF96144"/>
        <property type="gene ID" value="Metig_0590"/>
    </dbReference>
    <dbReference type="GeneID" id="10643428"/>
    <dbReference type="KEGG" id="mig:Metig_0590"/>
    <dbReference type="HOGENOM" id="CLU_110989_1_0_2"/>
    <dbReference type="OMA" id="QFNEGAK"/>
    <dbReference type="OrthoDB" id="111461at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.110.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01122">
        <property type="entry name" value="AlbA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036882">
        <property type="entry name" value="Alba-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013795">
        <property type="entry name" value="DNA/RNA-bd_Alba"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002775">
        <property type="entry name" value="DNA/RNA-bd_Alba-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01918">
        <property type="entry name" value="Alba"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028732">
        <property type="entry name" value="Alba"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82704">
        <property type="entry name" value="SSF82704"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00285">
        <property type="entry name" value="TIGR00285"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0007">Acetylation</keyword>
    <keyword evidence="2 7" id="KW-0158">Chromosome</keyword>
    <keyword evidence="3 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Alba" evidence="8">
        <location>
            <begin position="5"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-acetyllysine" evidence="7">
        <location>
            <position position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AEF96144.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="91" mass="10098" checksum="BC3B298CA00338A3" modified="2011-07-27" version="1">MSDNNAVYIGNKSIMNYVLAVITQFNTQGAKEVKIKARGKAISRAVDVAEMVRNRFLTDVKVKEINIGTDKIENEDGKVVNVSTIEIVLTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCD9</accession>
    <name>F6BCD9_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">50S ribosomal protein L39e</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">rpl39e</name>
        <name evidence="5" type="ordered locus">Metig_0596</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein eL39 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96150.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798757.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0596"/>
    <dbReference type="EnsemblBacteria" id="AEF96150">
        <property type="protein sequence ID" value="AEF96150"/>
        <property type="gene ID" value="Metig_0596"/>
    </dbReference>
    <dbReference type="GeneID" id="10643434"/>
    <dbReference type="KEGG" id="mig:Metig_0596"/>
    <dbReference type="HOGENOM" id="CLU_181948_4_0_2"/>
    <dbReference type="OMA" id="KMRYWRR"/>
    <dbReference type="OrthoDB" id="131584at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1620.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00629">
        <property type="entry name" value="Ribosomal_L39e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000077">
        <property type="entry name" value="Ribosomal_L39"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023626">
        <property type="entry name" value="Ribosomal_L39e_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00832">
        <property type="entry name" value="Ribosomal_L39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48662">
        <property type="entry name" value="SSF48662"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 5" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEF96150.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="51" mass="6040" checksum="916260FA9F5B3BEB" modified="2011-07-27" version="1">MASNKPLGKKLRLAKALKQNRRVPLFVILKTKGRVRSHPKMRFWRRSKLKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCE9</accession>
    <name>F6BCE9_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">2,5-diamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate reductase</fullName>
            <ecNumber evidence="6">1.1.1.302</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">2,5-diamino-6-(5-phospho-D-ribosylamino)pyrimidin-4(3H)-one reductase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">2,5-diamino-6-ribitylamino-4(3H)-pyrimidinone 5'-phosphate synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">Metig_0606</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2,5-diamino-6-(1-D-ribitylamino)pyrimidin-4(3H)-one 5'-phosphate + NAD(+) = 2,5-diamino-6-(1-D-ribosylamino)pyrimidin-4(3H)-one 5'-phosphate + H(+) + NADH</text>
            <dbReference type="Rhea" id="RHEA:27274"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:58890"/>
            <dbReference type="ChEBI" id="CHEBI:59545"/>
            <dbReference type="EC" id="1.1.1.302"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>2,5-diamino-6-(1-D-ribitylamino)pyrimidin-4(3H)-one 5'-phosphate + NADP(+) = 2,5-diamino-6-(1-D-ribosylamino)pyrimidin-4(3H)-one 5'-phosphate + H(+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:27278"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:58890"/>
            <dbReference type="ChEBI" id="CHEBI:59545"/>
            <dbReference type="EC" id="1.1.1.302"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Cofactor biosynthesis; riboflavin biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the HTP reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.302" evidence="6"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96160.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798766.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0606"/>
    <dbReference type="EnsemblBacteria" id="AEF96160">
        <property type="protein sequence ID" value="AEF96160"/>
        <property type="gene ID" value="Metig_0606"/>
    </dbReference>
    <dbReference type="GeneID" id="10643444"/>
    <dbReference type="KEGG" id="mig:Metig_0606"/>
    <dbReference type="HOGENOM" id="CLU_036590_4_1_2"/>
    <dbReference type="OMA" id="CECGQEV"/>
    <dbReference type="OrthoDB" id="72994at2157"/>
    <dbReference type="UniPathway" id="UPA00275"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008703">
        <property type="term" value="F:5-amino-6-(5-phosphoribosylamino)uracil reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009231">
        <property type="term" value="P:riboflavin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.430.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024072">
        <property type="entry name" value="DHFR-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006401">
        <property type="entry name" value="Rib_reduct_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011549">
        <property type="entry name" value="RibD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002734">
        <property type="entry name" value="RibDG_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01872">
        <property type="entry name" value="RibD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53597">
        <property type="entry name" value="SSF53597"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01508">
        <property type="entry name" value="rib_reduct_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00227">
        <property type="entry name" value="ribD_Cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RibD_C" evidence="11">
        <location>
            <begin position="3"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="91"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00000692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012851"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00015035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00030073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00031630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AEF96160.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="220" mass="24794" checksum="71DFCDA7145A89CF" modified="2011-07-27" version="1">MKPYVISNVGMTLDGKLATIANDSRISGEEDLKRVHQIRKEVDGIMVGIGTVLKDDPRLTVHKIPAKKEDNPVRIVVDSKLRVPLNARVLNKDAKTIIATTEEINEEKEEKIKKLTEMGVEVIKVGKGKVDLRKLMEELYKRGIKKILLEGGGTLNWGMFKENLVDEVRVFIAPKIFGGKDAPTYVDGEGFKTVDECVKLELKNFYRMDDGIVLEFKVKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCG0</accession>
    <name>F6BCG0_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0621</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96171.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0621"/>
    <dbReference type="EnsemblBacteria" id="AEF96171">
        <property type="protein sequence ID" value="AEF96171"/>
        <property type="gene ID" value="Metig_0621"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0621"/>
    <dbReference type="HOGENOM" id="CLU_108786_0_0_2"/>
    <dbReference type="OMA" id="RAAPICY"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007501">
        <property type="entry name" value="DUF531"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04407">
        <property type="entry name" value="DUF531"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="16"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96171.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="221" mass="25287" checksum="28A3EDD9D483DA05" modified="2011-07-27" version="1">MLFATTNFLFLMLFLYFYYLIMKVSMLLIIYVIFCFLLILKVIFMQKKSLKRMTLILYNSYDKNRWHEAHKRAIARAAPICYAFDCNLAIMEFPCKKEDILSIETTIGNSGEYLHKLIEKNRFLIIDKYLPQLGIPIVATSKPDKKKAITPMDVVNLLTKKPCGILVGLGRHGLPKDIVKMGEYHLDITGKGVSLETCTAIATIPAVISTYINAKFGYQQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCL7</accession>
    <name>F6BCL7_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Methyltransferase cognate corrinoid protein</fullName>
            <ecNumber evidence="3">2.1.1.13</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_0678</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.1.1.13" evidence="3"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96228.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798831.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0678"/>
    <dbReference type="EnsemblBacteria" id="AEF96228">
        <property type="protein sequence ID" value="AEF96228"/>
        <property type="gene ID" value="Metig_0678"/>
    </dbReference>
    <dbReference type="GeneID" id="10643518"/>
    <dbReference type="KEGG" id="mig:Metig_0678"/>
    <dbReference type="HOGENOM" id="CLU_082102_2_0_2"/>
    <dbReference type="OMA" id="MTSTMDN"/>
    <dbReference type="OrthoDB" id="95903at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031419">
        <property type="term" value="F:cobalamin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008705">
        <property type="term" value="F:methionine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1240.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003759">
        <property type="entry name" value="Cbl-bd_cap"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006158">
        <property type="entry name" value="Cobalamin-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036724">
        <property type="entry name" value="Cobalamin-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036594">
        <property type="entry name" value="Meth_synthase_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02310">
        <property type="entry name" value="B12-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02607">
        <property type="entry name" value="B12-binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01018">
        <property type="entry name" value="B12-binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47644">
        <property type="entry name" value="SSF47644"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52242">
        <property type="entry name" value="SSF52242"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51332">
        <property type="entry name" value="B12_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51337">
        <property type="entry name" value="B12_BINDING_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="B12-binding N-terminal" evidence="2">
        <location>
            <begin position="1"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="domain" description="B12-binding" evidence="1">
        <location>
            <begin position="91"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF96228.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="222" mass="24368" checksum="04E7171079583E30" modified="2011-07-27" version="1">MVSTDILNELSESVVNLDENRALKAANEALKAGIPAHIAIMDGLCKGMKIVGDKYEKKEYFVPEVLIASNVMNKAIDVLKPHVKLDKDDIPAKVVIGVVEGDIHEIGKNIVAMMLEAGGFEVHDLGRNVPCEKFVDYAIEINADIIVMSTMMTTTMYNMKKVIDVLKERGVRDKFIVMIGGGPVSSAFAKEIGADAYTDNANEALKVAKELYKKRKGKNIKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCL8</accession>
    <name>F6BCL8_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Alanine--glyoxylate transaminase</fullName>
            <ecNumber evidence="3">2.6.1.44</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_0679</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <dbReference type="EC" id="2.6.1.44" evidence="3"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96229.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798832.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0679"/>
    <dbReference type="EnsemblBacteria" id="AEF96229">
        <property type="protein sequence ID" value="AEF96229"/>
        <property type="gene ID" value="Metig_0679"/>
    </dbReference>
    <dbReference type="GeneID" id="10643519"/>
    <dbReference type="KEGG" id="mig:Metig_0679"/>
    <dbReference type="HOGENOM" id="CLU_027686_1_1_2"/>
    <dbReference type="OMA" id="KNWLPIM"/>
    <dbReference type="OrthoDB" id="20398at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008453">
        <property type="term" value="F:alanine-glyoxylate transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000192">
        <property type="entry name" value="Aminotrans_V_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024169">
        <property type="entry name" value="SP_NH2Trfase/AEP_transaminase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00266">
        <property type="entry name" value="Aminotran_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000524">
        <property type="entry name" value="SPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotran_5" evidence="2">
        <location>
            <begin position="13"/>
            <end position="330"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF96229.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="382" mass="41932" checksum="28FD885D6725581B" modified="2011-07-27" version="1">MKINTDKLLMIPGPTMVPAEVLNAMALPIIGHRTAEFGELLVDTTEKMKKVFQTKNDTYIITGSGTAAMDMAILNVIDKGDKVLNIVNGNFGERFAKITLAHKGESIKLENEWGDIAEPEAVKELLDKHDDIKAVTVVHNETSTGVKNPIEEIGKIVKDYDALYIVDTVSSLGGDYVDVDKFNIDICVTGSQKCLAAPPGLSAITVSEKAWEVIEKTEKKSLYLDLREYKKRYDEKKQTPYTPAVSLIYALNVALDLVLEEGLENRFKRHEKLARATRAGLEAMGIELFAKERARSVTVTSAYLPEGVEDKEFRGLLSKKYNVVIAGGQAHLSGKIFRIGHMGTAKEVHILATLGAIEMALKDLGVKVEGSGVEAAKEILME</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCP6</accession>
    <name>F6BCP6_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Carbamoyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metig_0707</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NodU/CmcH family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96257.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798860.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0707"/>
    <dbReference type="EnsemblBacteria" id="AEF96257">
        <property type="protein sequence ID" value="AEF96257"/>
        <property type="gene ID" value="Metig_0707"/>
    </dbReference>
    <dbReference type="GeneID" id="10643547"/>
    <dbReference type="KEGG" id="mig:Metig_0707"/>
    <dbReference type="HOGENOM" id="CLU_014411_2_0_2"/>
    <dbReference type="OMA" id="RTEMDYL"/>
    <dbReference type="OrthoDB" id="34157at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009058">
        <property type="term" value="P:biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.870.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031730">
        <property type="entry name" value="Carbam_trans_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038152">
        <property type="entry name" value="Carbam_trans_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003696">
        <property type="entry name" value="Carbtransf_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16861">
        <property type="entry name" value="Carbam_trans_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02543">
        <property type="entry name" value="Carbam_trans_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="SSF53067"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Carbam_trans_N" evidence="2">
        <location>
            <begin position="2"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="domain" description="Carbam_trans_N" evidence="2">
        <location>
            <begin position="77"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="domain" description="Carbam_trans_C" evidence="3">
        <location>
            <begin position="354"/>
            <end position="518"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF16861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEF96257.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="519" mass="58413" checksum="B906AFB59E924B85" modified="2011-07-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCS3</accession>
    <name>F6BCS3_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Methyltransferase type 11</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Metig_0734</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96284.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0734"/>
    <dbReference type="EnsemblBacteria" id="AEF96284">
        <property type="protein sequence ID" value="AEF96284"/>
        <property type="gene ID" value="Metig_0734"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0734"/>
    <dbReference type="HOGENOM" id="CLU_014042_0_0_2"/>
    <dbReference type="OMA" id="VMDVFDY"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002478">
        <property type="entry name" value="PUA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019614">
        <property type="entry name" value="SAM-dep_methyl-trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10672">
        <property type="entry name" value="Methyltrans_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00359">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="SSF88697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 5" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PUA" evidence="4">
        <location>
            <begin position="9"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEF96284.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="395" mass="45460" checksum="FD4F7E9F9FCB410C" modified="2011-07-27" version="1">MKLFGDGMVKIEIDKRAYNSIQNFSRLIYTNAVVNKEDLPDKEDIITLTYKGKFVAKAIYSPKSVIIKVLTTKDEEINKEFFYNRIKKANDYRTKILNFKDTYRMIYAEADGLPTIILDKYNEIGAMQVSSKVMERYLDIIVECLEEFGLETIYVKRGKKGDKIKERVYGDKSKFETIIEEGYENQKPAKFKVNVKGHKTGFFLDQRNNRLDLRDYIKEGDRVLDICCYTGGFSVHAALEGAEVVGVDISNKAIEVAEENMELNGIPKDKYEFIVGDAFAVMEEMIEDGEKFDVVILDPPAFTTTKKNLKNALRAYSTLNYLGIKLAKRILVTCSCSHHVDRETFRKTVVSSAYRAKKELRQIGGYRTQSPDHIITMANKNLEYLKCLFFHINEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCV5</accession>
    <name>F6BCV5_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Methanogenesis marker protein 11</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0770</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96316.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798919.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0770"/>
    <dbReference type="EnsemblBacteria" id="AEF96316">
        <property type="protein sequence ID" value="AEF96316"/>
        <property type="gene ID" value="Metig_0770"/>
    </dbReference>
    <dbReference type="GeneID" id="10643610"/>
    <dbReference type="KEGG" id="mig:Metig_0770"/>
    <dbReference type="HOGENOM" id="CLU_065511_0_0_2"/>
    <dbReference type="OMA" id="NKTQNCV"/>
    <dbReference type="OrthoDB" id="67932at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013696">
        <property type="entry name" value="DUF1743"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017674">
        <property type="entry name" value="Methan_mark_11"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08489">
        <property type="entry name" value="DUF1743"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03280">
        <property type="entry name" value="methan_mark_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF1743" evidence="1">
        <location>
            <begin position="146"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96316.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="299" mass="32886" checksum="4A9B3EFED139206B" modified="2011-07-27" version="1">MEDLYYKRIVAMVDKGLNLVELVEEHPCPNGAEWVVYQYQRTSPLIVSAWREGNKHHFVTKIGKAELNLIPSLSAAGIEEVFLKGDNVHIVHAGLAGAGVGAEMRKKAENVVDVIIHQKGGGSKLGKAEVITPKMEKVIVGIDDTDTKEEGATWVLANEIGKVVEKKGYGYYMDHTIVQLYPGNPNKTQNCVSIALTFAVYPKYKYKIDDLIKKELKEKSLSDDTAMAIYYGITPSKSMKLFTLRAKRELVSIEDARAVAMQNDIRIIKVNGEGGIIGAVAALGLAEHHDIAAKLPEDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCV9</accession>
    <name>F6BCV9_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ATPase_AAA_core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0774</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96320.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798923.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96320">
        <property type="protein sequence ID" value="AEF96320"/>
        <property type="gene ID" value="Metig_0774"/>
    </dbReference>
    <dbReference type="GeneID" id="10643614"/>
    <dbReference type="KEGG" id="mig:Metig_0774"/>
    <dbReference type="HOGENOM" id="CLU_049298_0_0_2"/>
    <dbReference type="OMA" id="YFIDNPF"/>
    <dbReference type="OrthoDB" id="38516at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13304">
        <property type="entry name" value="AAA_21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATPase_AAA_core" evidence="1">
        <location>
            <begin position="21"/>
            <end position="320"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96320.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="386" mass="44626" checksum="48BF1E6750869179" modified="2011-07-27" version="1">MKFEFYNIGCIENVELELKPFTLIAGVNQTGKSFILKCIYGILNSHGFNISELDKVLEDILKKSNIKKEEVNKLLEESDLVGAIVLFLLGLGLSYKLIKRNREGLQNKLKWIFQQKNLGNIVNKFSNSNEGIIKIITKDEVYTIKIPKDKDEIKTDKDELILKAGWANFISSPILLDLEKGIATYREYYPNNYGIPDIYWDILRDIRNVGKAEKIELIDIYKKIENIIGGKFEYEMGKGFVYKRGDKEFHINLVAYGIKIFGIIQMLIERNLITKGSVLILEEPEVHLHPSLRFKLVDLLKDLANSGVYVVISTHSPEIVRYVEYLINTKKMDIKNCSFLHLKLNEKTLTSYGKSKGDYKELNEIMKSLTEDYFDIVVREELEMED</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-06-02" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCZ3</accession>
    <name>F6BCZ3_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0809</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96354.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798956.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96354">
        <property type="protein sequence ID" value="AEF96354"/>
        <property type="gene ID" value="Metig_0809"/>
    </dbReference>
    <dbReference type="GeneID" id="10643650"/>
    <dbReference type="KEGG" id="mig:Metig_0809"/>
    <dbReference type="HOGENOM" id="CLU_1529236_0_0_2"/>
    <dbReference type="OrthoDB" id="142156at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="153"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96354.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="175" mass="19682" checksum="FFB43B1234A6C3CA" modified="2011-07-27" version="1">MKEGWSIKPYHKVIFLILALMFITQSYAYLTVKYSILEANGIPLSSPYNFTVLVSKENTITNPIVEETPYARFVIYVENKNDEWNLYTNATTINGYSSIKYKAELFNASGEHPAGVDNNPTTVYGDSDGEFWYWGGIPDSDFGSNQTPVKSPIPLGAVILTLIIIPLITLRRMVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>F6BD15</accession>
    <name>F6BD15_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Imidazoleglycerol-phosphate dehydratase</fullName>
            <shortName evidence="5">IGPD</shortName>
            <ecNumber evidence="5">4.2.1.19</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">hisB</name>
        <name evidence="6" type="ordered locus">Metig_0831</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate = 3-(imidazol-4-yl)-2-oxopropyl phosphate + H2O</text>
            <dbReference type="Rhea" id="RHEA:11040"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:57766"/>
            <dbReference type="ChEBI" id="CHEBI:58278"/>
            <dbReference type="EC" id="4.2.1.19"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 5">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 6/9.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the imidazoleglycerol-phosphate dehydratase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.19" evidence="5"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96376.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798978.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0831"/>
    <dbReference type="EnsemblBacteria" id="AEF96376">
        <property type="protein sequence ID" value="AEF96376"/>
        <property type="gene ID" value="Metig_0831"/>
    </dbReference>
    <dbReference type="GeneID" id="10643672"/>
    <dbReference type="KEGG" id="mig:Metig_0831"/>
    <dbReference type="HOGENOM" id="CLU_044308_2_0_2"/>
    <dbReference type="OMA" id="ARHGLFD"/>
    <dbReference type="OrthoDB" id="91418at2157"/>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00011"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004424">
        <property type="term" value="F:imidazoleglycerol-phosphate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07914">
        <property type="entry name" value="IGPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00076">
        <property type="entry name" value="HisB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038494">
        <property type="entry name" value="IGPD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000807">
        <property type="entry name" value="ImidazoleglycerolP_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020565">
        <property type="entry name" value="ImidazoleglycerP_deHydtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23133">
        <property type="entry name" value="PTHR23133"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00475">
        <property type="entry name" value="IGPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00954">
        <property type="entry name" value="IGP_DEHYDRATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00955">
        <property type="entry name" value="IGP_DEHYDRATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 5" id="KW-0368">Histidine biosynthesis</keyword>
    <keyword evidence="4 5 6" id="KW-0456">Lyase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEF96376.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="190" mass="21308" checksum="403BEFAEC99BFE51" modified="2011-07-27" version="1">MRVVEVKRETNETKIELKINIDGTGKYDINTGIPFFDHVLSSFAKHGAFDLYIKVEGDLEVDDHHTVEDVGICLGMALDKIEKKNINRFGWAIVPMDEARAMVAIDLGGRPYVVDNYVPKRDKVGNLSTENVKHFFEALANNAKINIHFEVVGENEHHKIEALFKAFGVALDMATRIDERKGVVSTKGVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F6BD38</accession>
    <name>F6BD38_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ABC transporter related protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Metig_0855</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96399.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799001.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0855"/>
    <dbReference type="EnsemblBacteria" id="AEF96399">
        <property type="protein sequence ID" value="AEF96399"/>
        <property type="gene ID" value="Metig_0855"/>
    </dbReference>
    <dbReference type="GeneID" id="10643696"/>
    <dbReference type="KEGG" id="mig:Metig_0855"/>
    <dbReference type="HOGENOM" id="CLU_072513_0_0_2"/>
    <dbReference type="OMA" id="LADIECM"/>
    <dbReference type="OrthoDB" id="45551at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="1"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="36"/>
            <end position="43"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEF96399.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="259" mass="28928" checksum="D472398F4F9ED582" modified="2011-07-27" version="1">MDIKEITILGGYDKFGNPERVRELTIKKGEILGVVGPTGSGKSNLISDIEQLAQGDTISKRRILVNGEVPPVEMRRDPRKRRIAQLSQNMHFLADMTVEEFLLMHAKSRGIEKDGIVDKVIKLANKLTGEPIDKDCPLTILSGGQSRSLMVADIAVISDSPIVLIDEIENAGIKKHEALELLAGHGKIVLVITHDPVLALITDRRVVMKNGGMKEVIETTEEEKEVSRRLGEIDNWMLMLREKVRHGERLTLKELELFC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>F6BD47</accession>
    <name>F6BD47_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">50S ribosomal protein L13</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">rpl13</name>
        <name evidence="5" type="ordered locus">Metig_0864</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">This protein is one of the early assembly proteins of the 50S ribosomal subunit, although it is not seen to bind rRNA by itself. It is important during the early stages of 50S assembly.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 4">Belongs to the universal ribosomal protein uL13 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96408.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799010.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0864"/>
    <dbReference type="EnsemblBacteria" id="AEF96408">
        <property type="protein sequence ID" value="AEF96408"/>
        <property type="gene ID" value="Metig_0864"/>
    </dbReference>
    <dbReference type="GeneID" id="10643707"/>
    <dbReference type="KEGG" id="mig:Metig_0864"/>
    <dbReference type="HOGENOM" id="CLU_076922_1_0_2"/>
    <dbReference type="OMA" id="GMLPWKT"/>
    <dbReference type="OrthoDB" id="87367at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015934">
        <property type="term" value="C:large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00392">
        <property type="entry name" value="Ribosomal_L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1180.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01366">
        <property type="entry name" value="Ribosomal_L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005822">
        <property type="entry name" value="Ribosomal_L13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023563">
        <property type="entry name" value="Ribosomal_L13_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005755">
        <property type="entry name" value="Ribosomal_L13_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036899">
        <property type="entry name" value="Ribosomal_L13_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11545">
        <property type="entry name" value="PTHR11545"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11545:SF3">
        <property type="entry name" value="PTHR11545:SF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00572">
        <property type="entry name" value="Ribosomal_L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002181">
        <property type="entry name" value="Ribosomal_L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52161">
        <property type="entry name" value="SSF52161"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01077">
        <property type="entry name" value="L13_A_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00783">
        <property type="entry name" value="RIBOSOMAL_L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="1 3 4" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003877"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEF96408.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="137" mass="15670" checksum="1350D7EEA1614B76" modified="2011-07-27" version="1">MVVVNAENAVVGRLASYVAKMALQGEEVIVVNAEKAIITGNREYILKKYLQKRMRKSITNPRRMGPKYPRRPDDIVRRIIRGMLPYKKPKGREAFKRVKVYVGVPEGITIDVNMVHKPNTNKYITVGELSKWLGAKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>F6BD61</accession>
    <name>F6BD61_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_0879</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96422.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0879"/>
    <dbReference type="EnsemblBacteria" id="AEF96422">
        <property type="protein sequence ID" value="AEF96422"/>
        <property type="gene ID" value="Metig_0879"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0879"/>
    <dbReference type="HOGENOM" id="CLU_078976_0_0_2"/>
    <dbReference type="OrthoDB" id="84268at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018763">
        <property type="entry name" value="DUF2334"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011330">
        <property type="entry name" value="Glyco_hydro/deAcase_b/a-brl"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10096">
        <property type="entry name" value="DUF2334"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88713">
        <property type="entry name" value="SSF88713"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF96422.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="275" mass="32556" checksum="31D5B13EFC9115D1" modified="2011-07-27" version="1">MKLLIYVLILILFIFSVCTIQNHPKTTVMYVINETKPQTNLGTKSPIETSWGSYLFEIHYKHISNKNPNYTSPIILVHDVSPLYYNELKEIVKIIDKHNYQNRTYLFVIVNHDNRYNLKNYPKFIKFLHKLEDDGYHIELHGYNHIDGEFNCNKEIAEEKIKKSLEILNECGFSNISYILPPRYEISKDAENVMLSYNLTIITKNYILTKTEKIPIKNREYTWYLPKSFTKIMLILAEFDYKTSNHQFYLSIHPKSINYGGGLKFLDEFLNETTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>F6BD68</accession>
    <name>F6BD68_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Arginine--tRNA ligase</fullName>
            <ecNumber evidence="8">6.1.1.19</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Arginyl-tRNA synthetase</fullName>
            <shortName evidence="8">ArgRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">argS</name>
        <name evidence="12" type="ordered locus">Metig_0886</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>ATP + L-arginine + tRNA(Arg) = AMP + diphosphate + L-arginyl-tRNA(Arg)</text>
            <dbReference type="Rhea" id="RHEA:20301"/>
            <dbReference type="Rhea" id="RHEA-COMP:9658"/>
            <dbReference type="Rhea" id="RHEA-COMP:9673"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:32682"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78513"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.19"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8 9">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.19" evidence="8"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96429.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799031.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0886"/>
    <dbReference type="EnsemblBacteria" id="AEF96429">
        <property type="protein sequence ID" value="AEF96429"/>
        <property type="gene ID" value="Metig_0886"/>
    </dbReference>
    <dbReference type="GeneID" id="10643730"/>
    <dbReference type="KEGG" id="mig:Metig_0886"/>
    <dbReference type="HOGENOM" id="CLU_006406_6_1_2"/>
    <dbReference type="OMA" id="NKPLHLG"/>
    <dbReference type="OrthoDB" id="7046at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004814">
        <property type="term" value="F:arginine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006420">
        <property type="term" value="P:arginyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00671">
        <property type="entry name" value="ArgRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00123">
        <property type="entry name" value="Arg_tRNA_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001278">
        <property type="entry name" value="Arg-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005148">
        <property type="entry name" value="Arg-tRNA-synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036695">
        <property type="entry name" value="Arg-tRNA-synth_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035684">
        <property type="entry name" value="ArgRS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008909">
        <property type="entry name" value="DALR_anticod-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11956">
        <property type="entry name" value="PTHR11956"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03485">
        <property type="entry name" value="Arg_tRNA_synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05746">
        <property type="entry name" value="DALR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00750">
        <property type="entry name" value="tRNA-synt_1d"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01038">
        <property type="entry name" value="TRNASYNTHARG"/>
    </dbReference>
    <dbReference type="SMART" id="SM01016">
        <property type="entry name" value="Arg_tRNA_synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00836">
        <property type="entry name" value="DALR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="SSF47323"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55190">
        <property type="entry name" value="SSF55190"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00456">
        <property type="entry name" value="argS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8 9" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="5 8 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 8 9" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 8 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 8 9" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Arg_tRNA_synt_N" evidence="11">
        <location>
            <begin position="4"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="domain" description="DALR_1" evidence="10">
        <location>
            <begin position="449"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'HIGH' region" evidence="8">
        <location>
            <begin position="120"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU363038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM01016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AEF96429.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="565" mass="64417" checksum="4A74231FC6143C7D" modified="2011-07-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>F6BD85</accession>
    <name>F6BD85_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">30S ribosomal protein S8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">rps8</name>
        <name evidence="6" type="ordered locus">Metig_0903</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">One of the primary rRNA binding proteins, it binds directly to 16S rRNA central domain where it helps coordinate assembly of the platform of the 30S subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Part of the 30S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4 5">Belongs to the universal ribosomal protein uS8 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96446.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799048.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0903"/>
    <dbReference type="EnsemblBacteria" id="AEF96446">
        <property type="protein sequence ID" value="AEF96446"/>
        <property type="gene ID" value="Metig_0903"/>
    </dbReference>
    <dbReference type="GeneID" id="10643747"/>
    <dbReference type="KEGG" id="mig:Metig_0903"/>
    <dbReference type="HOGENOM" id="CLU_098428_1_1_2"/>
    <dbReference type="OMA" id="LPAKNFG"/>
    <dbReference type="OrthoDB" id="91154at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01302_A">
        <property type="entry name" value="Ribosomal_S8_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000630">
        <property type="entry name" value="Ribosomal_S8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035987">
        <property type="entry name" value="Ribosomal_S8_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11758">
        <property type="entry name" value="PTHR11758"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00410">
        <property type="entry name" value="Ribosomal_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56047">
        <property type="entry name" value="SSF56047"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00053">
        <property type="entry name" value="RIBOSOMAL_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 5" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="4" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0699">rRNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEF96446.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="130" mass="14735" checksum="CF61DE496A82E8E3" modified="2011-07-27" version="1">MSLMDPLANALNHLTNCERVGKKVVYIKPASKLIGRVLKVMQDHGYIGEFEFIEDGRAGIFKVELIGKINKCGAIKPRYAVKKHEFEKFEKRYLPARDFGLLIVSTSQGIMTHYEAKEKGIGGRLISYVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>F6BD89</accession>
    <name>F6BD89_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">50S ribosomal protein L24</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">rpl24</name>
        <name evidence="4" type="ordered locus">Metig_0907</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Located at the polypeptide exit tunnel on the outside of the subunit.</text>
    </comment>
    <comment type="function">
        <text evidence="2">One of two assembly initiator proteins, it binds directly to the 5'-end of the 23S rRNA, where it nucleates assembly of the 50S subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the universal ribosomal protein uL24 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96450.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0907"/>
    <dbReference type="EnsemblBacteria" id="AEF96450">
        <property type="protein sequence ID" value="AEF96450"/>
        <property type="gene ID" value="Metig_0907"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0907"/>
    <dbReference type="HOGENOM" id="CLU_093240_2_1_2"/>
    <dbReference type="OMA" id="VRIMRGD"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015934">
        <property type="term" value="C:large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06089">
        <property type="entry name" value="KOW_RPL26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01326_A">
        <property type="entry name" value="Ribosomal_L24_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005824">
        <property type="entry name" value="KOW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041988">
        <property type="entry name" value="KOW_RPL26/RPL24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_L2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005825">
        <property type="entry name" value="Ribosomal_L24/26_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005756">
        <property type="entry name" value="Ribosomal_L26/L24P_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11143">
        <property type="entry name" value="PTHR11143"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00467">
        <property type="entry name" value="KOW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16906">
        <property type="entry name" value="Ribosomal_L26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00739">
        <property type="entry name" value="KOW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="SSF50104"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01080">
        <property type="entry name" value="rplX_A_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01108">
        <property type="entry name" value="RIBOSOMAL_L24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="2" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="KOW" evidence="3">
        <location>
            <begin position="47"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEF96450.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="121" mass="14117" checksum="52A1B342ADD7C98B" modified="2011-07-27" version="1">MVALTKSKQPRKQRKALFNAPLHLRNKVMSAMLSKELKEKLGKNSLPIRKGDVVKIMRGDFKGHQGEVVKVDYKRYRIYVEGAVNYRQDGKESLYPIHPSNVMIVKLYEGDEKRFKQIKKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-06-02" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDA0</accession>
    <name>F6BDA0_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0918</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96461.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799063.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96461">
        <property type="protein sequence ID" value="AEF96461"/>
        <property type="gene ID" value="Metig_0918"/>
    </dbReference>
    <dbReference type="GeneID" id="10643762"/>
    <dbReference type="KEGG" id="mig:Metig_0918"/>
    <dbReference type="HOGENOM" id="CLU_2152648_0_0_2"/>
    <dbReference type="OMA" id="TGEANRM"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96461.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="100" mass="11076" checksum="591026F98CB5AC3A" modified="2011-07-27" version="1">MSIITIFILMGIGILIGYLLFGRKRYYEIKAPKYAEISRGSNFLETAGAVAAGVLAAEAIEHIAEDMMDDEIIEESMEEFVDDIGDDMGDFVDDIGDLEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-09-29" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDA3</accession>
    <name>F6BDA3_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="3">AAA ATPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_0921</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the archaeal ATPase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96464.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799066.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96464">
        <property type="protein sequence ID" value="AEF96464"/>
        <property type="gene ID" value="Metig_0921"/>
    </dbReference>
    <dbReference type="GeneID" id="10643765"/>
    <dbReference type="KEGG" id="mig:Metig_0921"/>
    <dbReference type="HOGENOM" id="CLU_068608_0_0_2"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011579">
        <property type="entry name" value="ATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01637">
        <property type="entry name" value="ATPase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA" evidence="2">
        <location>
            <begin position="21"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF96464.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="360" mass="42908" checksum="84FC6E840FD1AEA8" modified="2011-07-27" version="1">MKFFNREKEIKEILSILEDEPNNIYFIFGSINSGKTTLIKHIIENKLNRDKYVVFYINLREHFISRYEDFIEVLFNTYEESFIEKIKRYFLSFIKDLPNNIDINSTILTGIPIPKNLINEFLSKKNSENVFKYLTKIFEDVKRKGKQPILIIDELQKIGDLKINGFLIYELFNFFIDLTKEKHICHVLCLSSDSLFIEKVYDEAMLRDRVDYILVDDFDEETALKFINFYAKEKNIKLDKKEKELIYSYVGGKPILIIKVINKMKIKNLSDILDELLKVEISKLENLLEDIKEGNYNVNYEEVIESLKLFKNTYSIPKSKIKKDIRNLLIKKNYLFLNPVNNEIKPQSFLIWNAIKEFYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDA8</accession>
    <name>F6BDA8_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CoB--CoM heterodisulfide reductase iron-sulfur subunit A</fullName>
            <ecNumber evidence="4">1.8.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Metig_0926</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Part of a complex that catalyzes the reversible reduction of CoM-S-S-CoB to the thiol-coenzymes H-S-CoM (coenzyme M) and H-S-CoB (coenzyme B).</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 4">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4">Cofactor metabolism; coenzyme M-coenzyme B heterodisulfide reduction; coenzyme B and coenzyme M from coenzyme M-coenzyme B heterodisulfide: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">The ferredoxin:CoB-CoM heterodisulfide reductase is composed of three subunits; HdrA, HdrB and HdrC.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the HdrA family.</text>
    </comment>
    <dbReference type="EC" id="1.8.-.-" evidence="4"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96469.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0926"/>
    <dbReference type="KEGG" id="mig:Metig_0926"/>
    <dbReference type="HOGENOM" id="CLU_020302_0_0_2"/>
    <dbReference type="OMA" id="CPSGAMQ"/>
    <dbReference type="UniPathway" id="UPA00647">
        <property type="reaction ID" value="UER00700"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039650">
        <property type="entry name" value="HdrA-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43498">
        <property type="entry name" value="PTHR43498"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12838">
        <property type="entry name" value="Fer4_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13187">
        <property type="entry name" value="Fer4_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="3 4" id="KW-0274">FAD</keyword>
    <keyword evidence="4" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0711">Selenium</keyword>
    <keyword evidence="6" id="KW-0712">Selenocysteine</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="5">
        <location>
            <begin position="236"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="5">
        <location>
            <begin position="284"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="5">
        <location>
            <begin position="575"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="5">
        <location>
            <begin position="608"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="non-standard amino acid" description="Selenocysteine" evidence="6">
        <location>
            <position position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU366072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEF96469.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="659" mass="71946" checksum="F0D145E9674DC884" modified="2011-07-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDE9</accession>
    <name>F6BDE9_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Metig_0967</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96510.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799112.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96510">
        <property type="protein sequence ID" value="AEF96510"/>
        <property type="gene ID" value="Metig_0967"/>
    </dbReference>
    <dbReference type="GeneID" id="10643813"/>
    <dbReference type="KEGG" id="mig:Metig_0967"/>
    <dbReference type="HOGENOM" id="CLU_1599045_0_0_2"/>
    <dbReference type="OMA" id="TCSLEMA"/>
    <dbReference type="OrthoDB" id="124884at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026432">
        <property type="entry name" value="Archaeo_ArtD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026392">
        <property type="entry name" value="Exo/Archaeosortase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019127">
        <property type="entry name" value="Exosortase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09721">
        <property type="entry name" value="Exosortase_EpsH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR04175">
        <property type="entry name" value="archaeo_artD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR04178">
        <property type="entry name" value="exo_archaeo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="6"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="58"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="90"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="125"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AEF96510.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="152" mass="17630" checksum="FE0DC7488A030036" modified="2011-07-27" version="1">MRDNKKYLLNFFVLLILIYLFLKNFEMEIAKLLSFVVGNILNIPYFENNLIYNGKVFSITSACTCLFELALFLSYVFATPKAPLKYKLAYVIFGILIINITNISRIIFILKNSNLADYHLIHDAISFIIFPVTLILNTIWVKILLKIKVVKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-06-02" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDF5</accession>
    <name>F6BDF5_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0973</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96516.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96516">
        <property type="protein sequence ID" value="AEF96516"/>
        <property type="gene ID" value="Metig_0973"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0973"/>
    <dbReference type="HOGENOM" id="CLU_1253590_0_0_2"/>
    <dbReference type="OMA" id="SCQLEWI"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96516.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="220" mass="26509" checksum="3F914CD126B8BA42" modified="2011-07-27" version="1">MIQRDGVGRFTMKYYTSLLICIFIVLFCGCYEKPYNKLEYHNTIINNNVIHNNMFLDHDRPKSYPNMYRFPKNYYKISDEMYPDVKKRDLPTLKYILNTIKLPPYEKNYYDCSEASCQLEWILEGYGFKVYLASGTRHTWLLVQLDSGEMVAVESTLLCENNYNPDCAIRYENYYYHPEFYADTPDMFLIPHNNKRMLITQLDWWNHPKNIKIKKKIFNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDG9</accession>
    <name>F6BDG9_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 9">Translation initiation factor 2 subunit alpha</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7 9">aIF2-alpha</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8 9">eIF-2-alpha</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">eif2a</name>
        <name evidence="11" type="ordered locus">Metig_0988</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 9">eIF-2 functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 9">Heterotrimer composed of an alpha, a beta and a gamma chain.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the eIF-2-alpha family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96530.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0988"/>
    <dbReference type="EnsemblBacteria" id="AEF96530">
        <property type="protein sequence ID" value="AEF96530"/>
        <property type="gene ID" value="Metig_0988"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0988"/>
    <dbReference type="HOGENOM" id="CLU_033458_0_2_2"/>
    <dbReference type="OMA" id="DVNEHQR"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04452">
        <property type="entry name" value="S1_IF2_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00231">
        <property type="entry name" value="eIF_2_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022967">
        <property type="entry name" value="S1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003029">
        <property type="entry name" value="S1_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044126">
        <property type="entry name" value="S1_IF2_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022964">
        <property type="entry name" value="TIF2_asu_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024055">
        <property type="entry name" value="TIF2_asu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024054">
        <property type="entry name" value="TIF2_asu_middle_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011488">
        <property type="entry name" value="TIF_2_asu"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10602">
        <property type="entry name" value="PTHR10602"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07541">
        <property type="entry name" value="EIF_2_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00575">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00316">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110993">
        <property type="entry name" value="SSF110993"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF116742">
        <property type="entry name" value="SSF116742"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50126">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9 11" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="6 9" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="S1 motif" evidence="10">
        <location>
            <begin position="23"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00030860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00033333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AEF96530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="274" mass="31218" checksum="60839AF9F39B6B86" modified="2011-07-27" version="1">MVLLSKIVIIGGVFMRRDFPEEGDIVIGTVIDVKPFGAFVELLEYPGKEGMVHISEVSSGWIKNIRDYIKKGQRVVAKVMRVNKEKGQIDLSLKRVTEQQKRAKVQEWKRFQRAEKMLEMAAQKLGKSLEEAWEEVGYLLEEEFGELYSAFEGLVIEGKDLLADLDIPQEWVDVLYEIAKENIELTNVKVEGFVELKSLAPNGVKIIKKALTNALKANPYEDVEVKITYVGSPKYRIEVIAPDYKSGEEVLKKVADKAIEYMEKHGGEGKFYRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDH6</accession>
    <name>F6BDH6_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcriptional regulator, TrmB</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_0995</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96537.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0995"/>
    <dbReference type="EnsemblBacteria" id="AEF96537">
        <property type="protein sequence ID" value="AEF96537"/>
        <property type="gene ID" value="Metig_0995"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0995"/>
    <dbReference type="HOGENOM" id="CLU_523401_0_0_2"/>
    <dbReference type="OMA" id="GFVFFDW"/>
    <dbReference type="OrthoDB" id="13479at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019494">
        <property type="entry name" value="FIST_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013702">
        <property type="entry name" value="FIST_domain_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002831">
        <property type="entry name" value="Tscrpt_reg_TrmB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08495">
        <property type="entry name" value="FIST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10442">
        <property type="entry name" value="FIST_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01978">
        <property type="entry name" value="TrmB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00897">
        <property type="entry name" value="FIST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01204">
        <property type="entry name" value="FIST_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FIST" evidence="1">
        <location>
            <begin position="38"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="domain" description="FIST_C" evidence="2">
        <location>
            <begin position="239"/>
            <end position="409"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF96537.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="532" mass="60926" checksum="2DC7A64B22B86A2B" modified="2011-07-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDH8</accession>
    <name>F6BDH8_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Small GTP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_0997</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96539.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799141.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0997"/>
    <dbReference type="EnsemblBacteria" id="AEF96539">
        <property type="protein sequence ID" value="AEF96539"/>
        <property type="gene ID" value="Metig_0997"/>
    </dbReference>
    <dbReference type="GeneID" id="10643844"/>
    <dbReference type="KEGG" id="mig:Metig_0997"/>
    <dbReference type="HOGENOM" id="CLU_077970_2_1_2"/>
    <dbReference type="OMA" id="RFEFMRE"/>
    <dbReference type="OrthoDB" id="80071at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF96539.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="162" mass="17870" checksum="98FF429F34AC9B3D" modified="2011-07-27" version="1">MSKEKRKNEIKVVVVGSSDVGKTTLMENLIGKIGKVEYKGTTVAIDYGSIEINNKKIHFFGTPGQKRFEFMRDIALNGADFVVLVIDATVSLGGVDREIIDALKNKGIPYLIFINKTDLVEKSKIEEIKNELKSLNVDILEGSALNREGIDKIVGCILSQVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDJ0</accession>
    <name>F6BDJ0_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Beta-lactamase domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1009</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96551.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799153.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1009"/>
    <dbReference type="EnsemblBacteria" id="AEF96551">
        <property type="protein sequence ID" value="AEF96551"/>
        <property type="gene ID" value="Metig_1009"/>
    </dbReference>
    <dbReference type="GeneID" id="10643863"/>
    <dbReference type="KEGG" id="mig:Metig_1009"/>
    <dbReference type="HOGENOM" id="CLU_030571_5_4_2"/>
    <dbReference type="OMA" id="GAWGTNC"/>
    <dbReference type="OrthoDB" id="103066at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="SSF56281"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Lactamase_B" evidence="1">
        <location>
            <begin position="11"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96551.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="205" mass="22967" checksum="A12C36430FC2A8C4" modified="2011-07-27" version="1">MIYKLNGVYYDANSYLIVGKKNILIDPGTPGNFEFLKNQIEEIVKDIDLVINTHCHYDHAGSDYLFEEYFDAPTIIHDSEVEHLKNGDKVTVSWLFNATFNPPKEIIPISEVKDELNKVGIEVIYTPGHTKGSISLVVEDKMITGDTLFAYGVGRWDLPTGDLAELRASIEKLERIAYQKGIAQIYPGHGEVGDLGAFGRAKMFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDK5</accession>
    <name>F6BDK5_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Probable cobalt-precorrin-6B C(15)-methyltransferase (decarboxylating)</fullName>
            <ecNumber evidence="3">2.1.1.196</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">cbiT</name>
        <name evidence="5" type="ordered locus">Metig_1024</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the methylation of C-15 in cobalt-precorrin-6B followed by the decarboxylation of C-12 to form cobalt-precorrin-7.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>Co-precorrin-6B + S-adenosyl-L-methionine = Co-precorrin-7 + CO2 + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:36067"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:70791"/>
            <dbReference type="ChEBI" id="CHEBI:72780"/>
            <dbReference type="EC" id="2.1.1.196"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Cofactor biosynthesis; adenosylcobalamin biosynthesis; cob(II)yrinate a,c-diamide from sirohydrochlorin (anaerobic route): step 8/10.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the methyltransferase superfamily. Archaeal-type CbiT family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.196" evidence="3"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96566.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799168.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1024"/>
    <dbReference type="EnsemblBacteria" id="AEF96566">
        <property type="protein sequence ID" value="AEF96566"/>
        <property type="gene ID" value="Metig_1024"/>
    </dbReference>
    <dbReference type="GeneID" id="10643878"/>
    <dbReference type="KEGG" id="mig:Metig_1024"/>
    <dbReference type="HOGENOM" id="CLU_094143_0_0_2"/>
    <dbReference type="OMA" id="RCKFVYA"/>
    <dbReference type="OrthoDB" id="82864at2157"/>
    <dbReference type="UniPathway" id="UPA00148">
        <property type="reaction ID" value="UER00229"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043776">
        <property type="term" value="F:cobalt-precorrin-6B C5-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008276">
        <property type="term" value="F:protein methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046140">
        <property type="term" value="P:corrin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00786">
        <property type="entry name" value="CbiT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023475">
        <property type="entry name" value="CbiT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014008">
        <property type="entry name" value="Cbl_synth_MTase_CbiT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025714">
        <property type="entry name" value="Methyltranfer_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13847">
        <property type="entry name" value="Methyltransf_31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02469">
        <property type="entry name" value="CbiT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3" id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword evidence="2 3 5" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltranfer_dom" evidence="4">
        <location>
            <begin position="31"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="S-adenosyl-L-methionine binding" evidence="3">
        <location>
            <begin position="40"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="16"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="61"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="89"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF13847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEF96566.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="181" mass="20094" checksum="2A389D93FB2CCB59" modified="2011-07-27" version="1">MIPDDEFIRVEGVPITKEEIRAVSIGKLRLNKDDVVVDIGCGSGGMTVEIAKRCKFVYAIDVDDKAIETTTKNLKKFGIKNCKVIKGNAKEILNDIDFNKAFIGGTKDIEEIIKTLNKKEIKHIVANTIVLENAIRVISLLEKYNYNVEVVNVSVSYGKKISIGYMMMAKNPITIITGKKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-06-02" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDV6</accession>
    <name>F6BDV6_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1128</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96667.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799268.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96667">
        <property type="protein sequence ID" value="AEF96667"/>
        <property type="gene ID" value="Metig_1128"/>
    </dbReference>
    <dbReference type="GeneID" id="10643986"/>
    <dbReference type="KEGG" id="mig:Metig_1128"/>
    <dbReference type="HOGENOM" id="CLU_1405969_0_0_2"/>
    <dbReference type="OMA" id="ILIIPDY"/>
    <dbReference type="OrthoDB" id="89679at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="81"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96667.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="188" mass="21787" checksum="E44C4743FE026784" modified="2011-07-27" version="1">MKIGITKMYEDIANLIGLKDYEIVNPYKEKCNCDFLIISKGYKEGVKKLNPNAEIFEVKSATFKDLIETLEELKKLGIGNKEKINNSIEFLKNKEKRIKKLAKNLNVKVNPKTDFVRKVVEDLNLEISDDGILIIPDYLFDGKERDDAIILKTHNYDLGLVERIEDRYLQMVNNLRGFYEGSYKGKCK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDX9</accession>
    <name>F6BDX9_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Transposase, IS605 OrfB family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Metig_1151</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96690.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799291.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1151"/>
    <dbReference type="EnsemblBacteria" id="AEF96690">
        <property type="protein sequence ID" value="AEF96690"/>
        <property type="gene ID" value="Metig_1151"/>
    </dbReference>
    <dbReference type="GeneID" id="10644009"/>
    <dbReference type="KEGG" id="mig:Metig_1151"/>
    <dbReference type="HOGENOM" id="CLU_032903_3_4_2"/>
    <dbReference type="OMA" id="GFPHIRS"/>
    <dbReference type="OrthoDB" id="75656at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032196">
        <property type="term" value="P:transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010095">
        <property type="entry name" value="Transposase_IS605_OrfB_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07282">
        <property type="entry name" value="OrfB_Zn_ribbon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01766">
        <property type="entry name" value="tspaseT_teng_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0815">Transposition</keyword>
    <feature type="domain" description="OrfB_Zn_ribbon" evidence="4">
        <location>
            <begin position="317"/>
            <end position="378"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF07282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEF96690.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="434" mass="51068" checksum="8CFC186143329AC2" modified="2011-07-27" version="1">MQNKNKLPSEIVLTYKIKHNYDLKNLLDGFMSTSQKAVDIIWENINWKEKQVKHRFKSKNKYKYYTTTRLIPEIPKDKDFKRELRNYLLNGWNFASHYVDGAIKVAYSTIESWKSNYLDGKRKRNKPTFKRPFVRVKNTLIKYDKENGTIRITIKARREYLVLNIKNEWFFEKIRGFDIGEIILKDNEALITFKKPLNIFDKKVVIGVDSNLKSLDLFHPEEGWIRVDLSELHRIKKVYDVIIDKLKSIYKKAPKRIGILLKKYYNRRKNRVEDFINKLTSQLSKLFPNAIFIFEDLDKLSMYKNSNFNRDLDRTSWREIAKKLEYKSIVFYVNPHYTSKTCPVCGSKMESQEGQVVTCEKCGVFNRQFVGSFNIFKRGVKLVKKLLGGVGAPVAGVEVDDLLPNEPRGELRPMSPKSIVRVNLSESTFIHIYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>F6BE52</accession>
    <name>F6BE52_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Helicase c2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Metig_0027</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95588.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798198.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0027"/>
    <dbReference type="EnsemblBacteria" id="AEF95588">
        <property type="protein sequence ID" value="AEF95588"/>
        <property type="gene ID" value="Metig_0027"/>
    </dbReference>
    <dbReference type="GeneID" id="10642862"/>
    <dbReference type="KEGG" id="mig:Metig_0027"/>
    <dbReference type="HOGENOM" id="CLU_012117_2_0_2"/>
    <dbReference type="OMA" id="QVNPVNE"/>
    <dbReference type="OrthoDB" id="31834at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003678">
        <property type="term" value="F:DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016818">
        <property type="term" value="F:hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006139">
        <property type="term" value="P:nucleobase-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006555">
        <property type="entry name" value="ATP-dep_Helicase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045028">
        <property type="entry name" value="DinG/Rad3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014013">
        <property type="entry name" value="Helic_SF1/SF2_ATP-bd_DinG/Rad3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006554">
        <property type="entry name" value="Helicase-like_DEXD_c2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006935">
        <property type="entry name" value="Helicase/UvrB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11472">
        <property type="entry name" value="PTHR11472"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13307">
        <property type="entry name" value="Helicase_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04851">
        <property type="entry name" value="ResIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00488">
        <property type="entry name" value="DEXDc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00491">
        <property type="entry name" value="HELICc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51193">
        <property type="entry name" value="HELICASE_ATP_BIND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0347">Helicase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="5">
        <location>
            <begin position="9"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="4">
        <location>
            <begin position="31"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEF95588.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="658" mass="76869" checksum="6933F1AA2E328AB3" modified="2011-07-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>F6BE67</accession>
    <name>F6BE67_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase</fullName>
            <ecNumber evidence="4">2.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">flpA</name>
        <name evidence="5" type="ordered locus">Metig_1206</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Involved in pre-rRNA and tRNA processing. Utilizes the methyl donor S-adenosyl-L-methionine to catalyze the site-specific 2'-hydroxyl methylation of ribose moieties in rRNA and tRNA. Site specificity is provided by a guide RNA that base pairs with the substrate. Methylation occurs at a characteristic distance from the sequence involved in base pairing with the guide RNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Interacts with nop5. Component of box C/D small ribonucleoprotein (sRNP) particles that contain rpl7ae, FlpA and nop5, plus a guide RNA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the methyltransferase superfamily. Fibrillarin family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="4"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96744.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799343.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1206"/>
    <dbReference type="EnsemblBacteria" id="AEF96744">
        <property type="protein sequence ID" value="AEF96744"/>
        <property type="gene ID" value="Metig_1206"/>
    </dbReference>
    <dbReference type="GeneID" id="10644066"/>
    <dbReference type="KEGG" id="mig:Metig_1206"/>
    <dbReference type="HOGENOM" id="CLU_059055_2_0_2"/>
    <dbReference type="OMA" id="INMATHR"/>
    <dbReference type="OrthoDB" id="58340at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00351">
        <property type="entry name" value="RNA_methyltransf_FlpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000692">
        <property type="entry name" value="Fibrillarin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020813">
        <property type="entry name" value="Fibrillarin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01269">
        <property type="entry name" value="Fibrillarin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006540">
        <property type="entry name" value="Nop17p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00052">
        <property type="entry name" value="FIBRILLARIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01206">
        <property type="entry name" value="Fibrillarin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00566">
        <property type="entry name" value="FIBRILLARIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4 5" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="2 4 5" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 4" id="KW-0819">tRNA processing</keyword>
    <feature type="region of interest" description="S-adenosyl-L-methionine binding" evidence="4">
        <location>
            <begin position="87"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="S-adenosyl-L-methionine binding" evidence="4">
        <location>
            <begin position="105"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="region of interest" description="S-adenosyl-L-methionine binding" evidence="4">
        <location>
            <begin position="130"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="S-adenosyl-L-methionine binding" evidence="4">
        <location>
            <begin position="150"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00351"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEF96744.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="228" mass="25932" checksum="F70CFFD4CF629B50" modified="2011-07-27" version="1">MEDIKIKEIFENIYEVDLGDGLRRIATKSLVEGKRVYGEKLIKVNGVEYRIWNPNKSKLAAAIINGLKVMPIKKGTKVLYLGASAGTTPSHVADIVEKSPIYSVEYSPRIMREFLEVCEDRKNLIPILGDANKPQEYAFIVEKVDVIYEDVAQPNQAEILIKNAKWFLKKGGYGMISIKARSIDVTKDPKEIFKEQKEILENGGFKIVDKVNIEPFEKDHVMFVGIWE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-06-02" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>F6BE77</accession>
    <name>F6BE77_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_1216</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96754.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799353.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96754">
        <property type="protein sequence ID" value="AEF96754"/>
        <property type="gene ID" value="Metig_1216"/>
    </dbReference>
    <dbReference type="GeneID" id="10644076"/>
    <dbReference type="KEGG" id="mig:Metig_1216"/>
    <dbReference type="HOGENOM" id="CLU_1192631_0_0_2"/>
    <dbReference type="OrthoDB" id="139907at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF96754.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="232" mass="27568" checksum="7975C6CA8379E70B" modified="2011-07-27" version="1">MIIILFEGVLDFLFFKSFFTKFLKFKNVGNEGNKEWFDKLLDYGYFEDLKNFDTSKIAVLREKGDKFEVMLISVGGKDSFKDAIRAFIEGHRILEKKIFGKIEKIIVVVDDDAKDKIGEIEKLVENKNLKDKFESLVLNGELEDVVLLIYEVIKDRLNDNDKKAIKEFFDIISKRYDEDKEKHNKKRKVSALHTAIGYRCYGHLFDELFEKFELDDLDNIEEFKKFYRIFKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>F6BE80</accession>
    <name>F6BE80_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">50S ribosomal protein L3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">rpl3</name>
        <name evidence="8" type="ordered locus">Metig_1220</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">One of the primary rRNA binding proteins, it binds directly near the 3'-end of the 23S rRNA, where it nucleates assembly of the 50S subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Part of the 50S ribosomal subunit. Forms a cluster with proteins L14 and L24e.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the universal ribosomal protein uL3 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96757.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799356.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1220"/>
    <dbReference type="EnsemblBacteria" id="AEF96757">
        <property type="protein sequence ID" value="AEF96757"/>
        <property type="gene ID" value="Metig_1220"/>
    </dbReference>
    <dbReference type="GeneID" id="10644081"/>
    <dbReference type="KEGG" id="mig:Metig_1220"/>
    <dbReference type="HOGENOM" id="CLU_033361_2_0_2"/>
    <dbReference type="OMA" id="HQRTEYN"/>
    <dbReference type="OrthoDB" id="31722at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.960.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01325_A">
        <property type="entry name" value="Ribosomal_L3_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045077">
        <property type="entry name" value="L3_arc_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000597">
        <property type="entry name" value="Ribosomal_L3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019928">
        <property type="entry name" value="Ribosomal_L3_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019926">
        <property type="entry name" value="Ribosomal_L3_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044892">
        <property type="entry name" value="Ribosomal_L3_dom_3_arc_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11363">
        <property type="entry name" value="PTHR11363"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00297">
        <property type="entry name" value="Ribosomal_L3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="SSF50447"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03626">
        <property type="entry name" value="L3_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00474">
        <property type="entry name" value="RIBOSOMAL_L3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 6 8" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3 6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2 6" id="KW-0699">rRNA-binding</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEF96757.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="335" mass="37956" checksum="BCFF16FCE29FB579" modified="2011-07-27" version="1">MGMKRNRPRRGSLAFSPRKRAKRPVPRIRSWPDEDKVRLQAFPVYKAGMSHALVKENNPNSPNANQEVFTPITILEAPDINVFAIRVYGKDTRGLKTLTEVWADELDKELARKIKTLPKKEERKTVDELDNLIDKIADVRVLVHTNPKLTCLPKKKPEVLEIRIGGKDIQERLNYAKEILGKKVSITDVFSEGEFVDTIAITKGKGFQGQVKRWGVKIQFGKHQRKGVGRHVGSIGPWTPKRVMWTVPMPGQMGYHQRTEYNKRILKIGNNGEEITPKGGFLHYGVIRNNYVVVKGSVPGPAKRLVVLRSAIRPVEDRFGLPEITYISTTSKQGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEA9</accession>
    <name>F6BEA9_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CRISPR system Cms endoribonuclease Csm3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">CRISPR type III A-associated RAMP protein Csm3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Metig_1249</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CRISPR-associated Csm3 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96786.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1249"/>
    <dbReference type="EnsemblBacteria" id="AEF96786">
        <property type="protein sequence ID" value="AEF96786"/>
        <property type="gene ID" value="Metig_1249"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_1249"/>
    <dbReference type="HOGENOM" id="CLU_067743_0_0_2"/>
    <dbReference type="OMA" id="ICKIFGP"/>
    <dbReference type="OrthoDB" id="98478at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013412">
        <property type="entry name" value="CRISPR-assoc_RAMP_Csm3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005537">
        <property type="entry name" value="RAMP_III_fam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03787">
        <property type="entry name" value="RAMPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02582">
        <property type="entry name" value="cas7_TM1809"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0051">Antiviral defense</keyword>
    <keyword evidence="4" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00033183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEF96786.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="342" mass="39393" checksum="405EEEEEF045136C" modified="2011-07-27" version="1">MKENKKVMKMENITLKGKVIISGVIELLTGLHIGGLKETLKIGGTDDPVIKDAFGRIYIPGSSLKGKIRCLLEKSMGKGKVKVVIKVKKEIKEGDKSRYEVKEDKEETYELKDFNTDIVDKLIEEYKSKGYTEDKGYSIESHITALPCSCGDCVICKIFGPHDSKKIEEPRRVVFRDAYLVKEINDNEKNRNNSNNEVIEIGEKKYKILDENDEELNEYLEIKPENIIDRVKGVAQHPRFIERVSAGAKFYFEIVFNVYKKEDKELLKELITGMKLLEDDYLGGSGSRGYGKIKFENLKIVNRPKEYYEGNEKAEKVTDVKDLDELKNLNKLEEKIKEIWNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEB0</accession>
    <name>F6BEB0_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CRISPR system Cms protein Csm4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Metig_1250</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CRISPR-associated Csm4 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96787.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799386.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1250"/>
    <dbReference type="EnsemblBacteria" id="AEF96787">
        <property type="protein sequence ID" value="AEF96787"/>
        <property type="gene ID" value="Metig_1250"/>
    </dbReference>
    <dbReference type="GeneID" id="10644113"/>
    <dbReference type="KEGG" id="mig:Metig_1250"/>
    <dbReference type="HOGENOM" id="CLU_062371_0_0_2"/>
    <dbReference type="OMA" id="NSKYHFG"/>
    <dbReference type="OrthoDB" id="68179at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005510">
        <property type="entry name" value="Csm4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040932">
        <property type="entry name" value="Csm4_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17953">
        <property type="entry name" value="Csm4_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01903">
        <property type="entry name" value="cas5_csm4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0051">Antiviral defense</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Csm4_C" evidence="5">
        <location>
            <begin position="265"/>
            <end position="355"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00016109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF17953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEF96787.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="358" mass="41689" checksum="97353CF5E902A2E6" modified="2011-07-27" version="1">MKKLVILKPSVNSKYHFGSGDLEKSDVIFHSSNLFSAIVNNFVLLYGEDYKEEINKKVEELAENLRLSSLFIKIKNTFLLPKPEYPIFYLLKNENYMKIKPKDVKKIKFVTLKAYKEIVMEKLKEIGYGSKNYYINCLFDNNLDEYFNKRVTKSIFATEEEIEEIKNIEIFKRYDEEKNTIDRICERPAEGQLYKVEFIKFDKNVELYFIIDYPEYLNKEIQASIRLLADEGLGGKRSIGSGHFEKVEIKDIKEFKDFNELFNTQGELNILLGVGIPKEDEISNIKYYKLLELGGYIYSTTKPELVTKHRRSLMALSEGSVVKAFEGCIKNVKPKNSSENIHPVYTHGKPITLPLVEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEB8</accession>
    <name>F6BEB8_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_1258</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96795.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799393.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1258"/>
    <dbReference type="EnsemblBacteria" id="AEF96795">
        <property type="protein sequence ID" value="AEF96795"/>
        <property type="gene ID" value="Metig_1258"/>
    </dbReference>
    <dbReference type="GeneID" id="10644121"/>
    <dbReference type="KEGG" id="mig:Metig_1258"/>
    <dbReference type="HOGENOM" id="CLU_160441_0_0_2"/>
    <dbReference type="OMA" id="QAYFVDP"/>
    <dbReference type="OrthoDB" id="113752at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008032">
        <property type="entry name" value="DUF749"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035933">
        <property type="entry name" value="MTH1880"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05370">
        <property type="entry name" value="DUF749"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75412">
        <property type="entry name" value="SSF75412"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF96795.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="101" mass="11689" checksum="374668B934F4DB86" modified="2011-07-27" version="1">MGEFVATLLTILKVEDGLNSELSDFIRVRAAIENRELKNDDVVAVFNIQGTTSYQVFFLDDDTTVEEIKEKLKKISVRLNYDSEEVLKRYINARRGKNERC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F6BED1</accession>
    <name>F6BED1_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">SirA-like domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1271</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96808.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799406.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1271"/>
    <dbReference type="EnsemblBacteria" id="AEF96808">
        <property type="protein sequence ID" value="AEF96808"/>
        <property type="gene ID" value="Metig_1271"/>
    </dbReference>
    <dbReference type="GeneID" id="10644134"/>
    <dbReference type="KEGG" id="mig:Metig_1271"/>
    <dbReference type="HOGENOM" id="CLU_165255_0_1_2"/>
    <dbReference type="OMA" id="VVGDYKP"/>
    <dbReference type="OrthoDB" id="124510at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.110.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001455">
        <property type="entry name" value="TusA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036868">
        <property type="entry name" value="TusA-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01206">
        <property type="entry name" value="TusA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64307">
        <property type="entry name" value="SSF64307"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01148">
        <property type="entry name" value="UPF0033"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UPF0033" evidence="1">
        <location>
            <begin position="3"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS01148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96808.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="70" mass="7824" checksum="AFC4692F32E27939" modified="2011-07-27" version="1">MKIDVTGDICPVPVLKTKKALEQLKEGEELEVVGDYKPALENIKRFAESNGYTVVTAEETENGFRIVIKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEE0</accession>
    <name>F6BEE0_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Isopropylmalate/isohomocitrate dehydrogenase</fullName>
            <ecNumber evidence="5">1.1.1.87</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Metig_0040</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the isocitrate and isopropylmalate dehydrogenases family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.87" evidence="5"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95601.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798210.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0040"/>
    <dbReference type="EnsemblBacteria" id="AEF95601">
        <property type="protein sequence ID" value="AEF95601"/>
        <property type="gene ID" value="Metig_0040"/>
    </dbReference>
    <dbReference type="GeneID" id="10642875"/>
    <dbReference type="KEGG" id="mig:Metig_0040"/>
    <dbReference type="HOGENOM" id="CLU_031953_0_1_2"/>
    <dbReference type="OMA" id="EYDLGAR"/>
    <dbReference type="OrthoDB" id="33452at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003862">
        <property type="term" value="F:3-isopropylmalate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047046">
        <property type="term" value="F:homoisocitrate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009098">
        <property type="term" value="P:leucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019818">
        <property type="entry name" value="IsoCit/isopropylmalate_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024084">
        <property type="entry name" value="IsoPropMal-DH-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011828">
        <property type="entry name" value="LEU3_arc"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00180">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01329">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02088">
        <property type="entry name" value="LEU3_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00470">
        <property type="entry name" value="IDH_IMDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Iso_dh" evidence="4">
        <location>
            <begin position="3"/>
            <end position="324"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM01329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEF95601.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="335" mass="37300" checksum="08EC2F155112CBAF" modified="2011-07-27" version="1">MKKICVIEGDGIGKEVVPAAIKVLDEVGDFEYIYAEAGLECLKKYGDAIPDETIEKAKECDAVLFGAITSPKPTEYKGKYRSPILTLRKELNLYANVRPINDFKNIDLVIIRENTEDVYVGREYYDEEKEIAIAERVISKKGSERIIKFAFEYAINNNRKKVSCIHKANVLRITDGLFLRIFNEIGEKYKDKIDYDDYLVDATAMYLVKSPEMFDVIVTTNLFGDILSDEASALIGGLGLAPSANIGDEYGLFEPVHGSAPDIAGKGIANPIATILSAAMMLDYFGMKEKGNKIRNAVKRVLEKGKVTPDLGGNLKTSEVVDEIIKELRGYSRVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEE2</accession>
    <name>F6BEE2_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Hydrogenase accessory protein HypB</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0042</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95603.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798212.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0042"/>
    <dbReference type="EnsemblBacteria" id="AEF95603">
        <property type="protein sequence ID" value="AEF95603"/>
        <property type="gene ID" value="Metig_0042"/>
    </dbReference>
    <dbReference type="GeneID" id="10642877"/>
    <dbReference type="KEGG" id="mig:Metig_0042"/>
    <dbReference type="HOGENOM" id="CLU_056148_0_1_2"/>
    <dbReference type="OMA" id="ECHLDAH"/>
    <dbReference type="OrthoDB" id="76245at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016151">
        <property type="term" value="F:nickel cation binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051604">
        <property type="term" value="P:protein maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05390">
        <property type="entry name" value="HypB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003495">
        <property type="entry name" value="CobW/HypB/UreG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004392">
        <property type="entry name" value="Hyd_mat_HypB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30134">
        <property type="entry name" value="PTHR30134"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02492">
        <property type="entry name" value="cobW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00073">
        <property type="entry name" value="hypB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="cobW" evidence="1">
        <location>
            <begin position="38"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF95603.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="224" mass="24533" checksum="6C6C1AEBF5342766" modified="2011-07-27" version="1">MHLVGVLSIGKDIIKANKKLADKNRKKLNEHGVVAFDFMGAIGSGKTALIEYLIDKLKDKYKIACIAGDVIAKFDAERMERHGVKVIPLNTGKECHLDAHLVSHALQDLNLDEIDVLFIENVGNLICPADFDLGTHKRIIVVSVTEGDDTVEKHPLIFKTADLAIINKVDLADAVGADAEKMKEDAKKINGDLEVILTSLKTGEGLDKVVDFIESAIKEVKENK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEH1</accession>
    <name>F6BEH1_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Probable deoxyhypusine synthase</fullName>
            <shortName evidence="7">DHS</shortName>
            <ecNumber evidence="7">2.5.1.46</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">dys</name>
        <name evidence="8" type="ordered locus">Metig_0071</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3 7">Catalyzes the NAD-dependent oxidative cleavage of spermidine and the subsequent transfer of the butylamine moiety of spermidine to the epsilon-amino group of a specific lysine residue of the eIF-5A precursor protein to form the intermediate deoxyhypusine residue.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>[eIF5A protein]-L-lysine + spermidine = [eIF5A protein]-deoxyhypusine + propane-1,3-diamine</text>
            <dbReference type="Rhea" id="RHEA:33299"/>
            <dbReference type="Rhea" id="RHEA-COMP:10143"/>
            <dbReference type="Rhea" id="RHEA-COMP:10144"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57484"/>
            <dbReference type="ChEBI" id="CHEBI:57834"/>
            <dbReference type="ChEBI" id="CHEBI:82657"/>
            <dbReference type="EC" id="2.5.1.46"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 7">
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4 7">Protein modification; eIF5A hypusination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5 7">Belongs to the deoxyhypusine synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.46" evidence="7"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95632.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798241.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0071"/>
    <dbReference type="EnsemblBacteria" id="AEF95632">
        <property type="protein sequence ID" value="AEF95632"/>
        <property type="gene ID" value="Metig_0071"/>
    </dbReference>
    <dbReference type="GeneID" id="10642906"/>
    <dbReference type="KEGG" id="mig:Metig_0071"/>
    <dbReference type="HOGENOM" id="CLU_039781_0_0_2"/>
    <dbReference type="OMA" id="FWSYFCQ"/>
    <dbReference type="OrthoDB" id="35308at2157"/>
    <dbReference type="UniPathway" id="UPA00354"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034038">
        <property type="term" value="F:deoxyhypusine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008612">
        <property type="term" value="P:peptidyl-lysine modification to peptidyl-hypusine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.910.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00153">
        <property type="entry name" value="DHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022899">
        <property type="entry name" value="Deoxyhypus_synthase_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002773">
        <property type="entry name" value="Deoxyhypusine_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036982">
        <property type="entry name" value="Deoxyhypusine_synthase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11703">
        <property type="entry name" value="PTHR11703"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01916">
        <property type="entry name" value="DS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="SSF52467"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00321">
        <property type="entry name" value="dhys"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0386">Hypusine biosynthesis</keyword>
    <keyword evidence="7" id="KW-0520">NAD</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 8" id="KW-0808">Transferase</keyword>
    <feature type="active site" description="Nucleophile" evidence="7">
        <location>
            <position position="309"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00002823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00009892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEF95632.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="337" mass="38237" checksum="009448FCDEC5EA62" modified="2011-07-27" version="1">MDPKDIVLKKSDTEGLKEIPIEGPWLDDEISLEDAVKNYYEKIGFQATHLGKAIKIWKKIEEKRKNGDITVFFGYTSNIVSSGLREIIAYLAKHKKIDVIVTTAGGIEEDFIKCLKPFILGDWNVNGKFLREKGINRIGNIFVPNDRYIEFETYMTEFFDRILEMEKKEQRVITASEFCYELGKFMDEKLGREKEKSILYWAYKNNIPIFCPAITDGSIGDMLYFYKKSKKDETLKIDVANDIVKLNDIAINAKETACIVLGGSLPKHSIINANLFREGTDYAIYITTAVPWDGSLSGAPPEEGVSWGKIGAKADYVEIWADATLVFPILVYCVMKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-09-29" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEI5</accession>
    <name>F6BEI5_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">PRC-barrel domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0085</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95646.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798255.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF95646">
        <property type="protein sequence ID" value="AEF95646"/>
        <property type="gene ID" value="Metig_0085"/>
    </dbReference>
    <dbReference type="GeneID" id="10642920"/>
    <dbReference type="KEGG" id="mig:Metig_0085"/>
    <dbReference type="HOGENOM" id="CLU_174517_0_0_2"/>
    <dbReference type="OMA" id="AEHSPIM"/>
    <dbReference type="OrthoDB" id="112213at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027275">
        <property type="entry name" value="PRC-brl_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011033">
        <property type="entry name" value="PRC_barrel-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05239">
        <property type="entry name" value="PRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50346">
        <property type="entry name" value="SSF50346"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PRC" evidence="1">
        <location>
            <begin position="5"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF95646.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="82" mass="8932" checksum="AD9AF7822FF9AC31" modified="2011-07-27" version="1">MKMPIRALFGRSIVGNLGSIIGTVEDIIIDEETGKVISLSVEPSPQSPIPPNDENYTLIPFKIVNAIKDVVVIDESKITKKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEJ9</accession>
    <name>F6BEJ9_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Hydroxylamine reductase</fullName>
            <ecNumber evidence="6">1.7.99.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Hybrid-cluster protein</fullName>
            <shortName evidence="6">HCP</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Prismane protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">hcp</name>
        <name evidence="7" type="ordered locus">Metig_0099</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes the reduction of hydroxylamine to form NH(3) and H(2)O.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>A + H2O + NH4(+) = AH2 + H(+) + hydroxylamine</text>
            <dbReference type="Rhea" id="RHEA:22052"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15429"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="EC" id="1.7.99.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="6">Binds 1 [4Fe-4S] cluster.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>hybrid [4Fe-2O-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:60519"/>
        </cofactor>
        <text evidence="6">Binds 1 hybrid [4Fe-2O-2S] cluster.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the HCP family.</text>
    </comment>
    <dbReference type="EC" id="1.7.99.1" evidence="6"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95660.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798269.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0099"/>
    <dbReference type="EnsemblBacteria" id="AEF95660">
        <property type="protein sequence ID" value="AEF95660"/>
        <property type="gene ID" value="Metig_0099"/>
    </dbReference>
    <dbReference type="GeneID" id="10642934"/>
    <dbReference type="KEGG" id="mig:Metig_0099"/>
    <dbReference type="HOGENOM" id="CLU_038344_2_0_2"/>
    <dbReference type="OMA" id="CAYAQGM"/>
    <dbReference type="OrthoDB" id="41554at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050418">
        <property type="term" value="F:hydroxylamine reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01914">
        <property type="entry name" value="HCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2030">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00069">
        <property type="entry name" value="Hydroxylam_reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004137">
        <property type="entry name" value="HCP/CODH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010048">
        <property type="entry name" value="Hydroxylam_reduct"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016099">
        <property type="entry name" value="Prismane-like_a/b-sand"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011254">
        <property type="entry name" value="Prismane-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016100">
        <property type="entry name" value="Prismane_a-bundle"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30109">
        <property type="entry name" value="PTHR30109"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30109:SF0">
        <property type="entry name" value="PTHR30109:SF0"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03063">
        <property type="entry name" value="Prismane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000076">
        <property type="entry name" value="HCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56821">
        <property type="entry name" value="SSF56821"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01703">
        <property type="entry name" value="hybrid_clust"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="1 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 6" id="KW-0408">Iron</keyword>
    <keyword evidence="5 6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="6">
        <location>
            <position position="12"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="15"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="24"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="30"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="244"/>
        </location>
        <ligand>
            <name>hybrid [4Fe-2O-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:60519"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="268"/>
        </location>
        <ligand>
            <name>hybrid [4Fe-2O-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:60519"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="312"/>
        </location>
        <ligand>
            <name>hybrid [4Fe-2O-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:60519"/>
        </ligand>
    </feature>
    <feature type="binding site" description="via persulfide group" evidence="6">
        <location>
            <position position="403"/>
        </location>
        <ligand>
            <name>hybrid [4Fe-2O-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:60519"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="431"/>
        </location>
        <ligand>
            <name>hybrid [4Fe-2O-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:60519"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="456"/>
        </location>
        <ligand>
            <name>hybrid [4Fe-2O-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:60519"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="491"/>
        </location>
        <ligand>
            <name>hybrid [4Fe-2O-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:60519"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="493"/>
        </location>
        <ligand>
            <name>hybrid [4Fe-2O-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:60519"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="Cysteine persulfide" evidence="6">
        <location>
            <position position="403"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEF95660.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="548" mass="61351" checksum="8896723D3539FBF7" modified="2011-07-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEK0</accession>
    <name>F6BEK0_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">4Fe-4S ferredoxin iron-sulfur binding domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0100</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95661.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF95661">
        <property type="protein sequence ID" value="AEF95661"/>
        <property type="gene ID" value="Metig_0100"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0100"/>
    <dbReference type="HOGENOM" id="CLU_074768_0_0_2"/>
    <dbReference type="OMA" id="KMEVPCC"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12837">
        <property type="entry name" value="Fer4_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="10"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="44"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF95661.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="232" mass="26109" checksum="3F7274B68D03CB23" modified="2011-07-27" version="1">MVIFMKITRYRVEVDQDKCIGYEECGLCIKACEENILIPDPKTKKVKVNKEREIYCDGIGSCLDVCPVDAIKIIKEEVEVDEHHSHSGHKCPGSMAMTLKRDRKSDEDDNVEMGSELTNWPIQLHLLNPMAPYFKNAELVISADCVPFAYANFHKKFLKGKVLAIGCPKLDDTSEYAEKIRAIVKLNNIKKVDVVIMSVPCCHGLYALVKKALDGLDCEIKRHIISIDGKAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEK3</accession>
    <name>F6BEK3_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">GDP dissociation inhibitor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_0103</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95664.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0103"/>
    <dbReference type="EnsemblBacteria" id="AEF95664">
        <property type="protein sequence ID" value="AEF95664"/>
        <property type="gene ID" value="Metig_0103"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0103"/>
    <dbReference type="HOGENOM" id="CLU_668371_0_0_2"/>
    <dbReference type="OMA" id="ALHMIPH"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF95664.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="441" mass="48879" checksum="29A16B3D994DB2F6" modified="2011-07-27" version="1">MKNVYLDNLLLINVHKCPCIIKQFQTIIQHYDFMRDNMRIGIVGGGLGGLLTAALLSKNHDVVIFEKLPFVGGRFTNIEYKGFQLTTGALHMIPHGNDGYLARLLRECGCNVKIINSNPDGLFRVNGKDYAYKDLFNLVGLKDKLKALKMAANLKIGNVDKSMSFGEFLESVDLALKVGNAFTGWALSLTAYETPMEEIIEIAKNYHKFGGPGIPIGGCKGVIDELVRAIKNNNGIIKVECEVKSIEIEDDKAYVIGEELNEEFDVVVSNLSPKLTEKISNVKIIKGKEPRPSKGIKICVATKEGLIKHNGVLFTPECERINGLNQVTNVDKSLAPEGYHLVMTHQTQLTNNVKKEIDLGLEDIERLFNGKDYEILHIQSYRDEWPVNHASNGTDLDNIVNDKLYLVGDGAKGKGGIEVEGIAMGVMKVVEYINSLNKTKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEL0</accession>
    <name>F6BEL0_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Rubrerythrin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0110</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95671.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798280.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0110"/>
    <dbReference type="EnsemblBacteria" id="AEF95671">
        <property type="protein sequence ID" value="AEF95671"/>
        <property type="gene ID" value="Metig_0110"/>
    </dbReference>
    <dbReference type="GeneID" id="10642945"/>
    <dbReference type="KEGG" id="mig:Metig_0110"/>
    <dbReference type="HOGENOM" id="CLU_113705_0_0_2"/>
    <dbReference type="OMA" id="NFEGECK"/>
    <dbReference type="OrthoDB" id="104203at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01046">
        <property type="entry name" value="Rubrerythrin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1260.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012347">
        <property type="entry name" value="Ferritin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009040">
        <property type="entry name" value="Ferritin-like_diiron"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009078">
        <property type="entry name" value="Ferritin-like_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003251">
        <property type="entry name" value="Rubrerythrin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045236">
        <property type="entry name" value="Rubrerythrin-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02915">
        <property type="entry name" value="Rubrerythrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47240">
        <property type="entry name" value="SSF47240"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50905">
        <property type="entry name" value="FERRITIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ferritin-like diiron" evidence="1">
        <location>
            <begin position="13"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF95671.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="141" mass="15938" checksum="4B574869D2BBD5D0" modified="2011-07-27" version="1">MELELINEHKIGVTKGTELEKEVQANFEGECKEVGLYLAMARQAQREGLPEVAEVLMRIAMEEAEHAAHFAEMNGIISENLKENIEMMLKGECMANKEKKAAATKAKELGIDPAHDFFDESSRDEARHAKMLKGILDRYFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEL8</accession>
    <name>F6BEL8_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">N-glycosylase/DNA lyase</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">8-oxoguanine DNA glycosylase</fullName>
            <ecNumber evidence="2">3.2.2.-</ecNumber>
        </alternativeName>
        <alternativeName>
            <fullName evidence="2">AGOG</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="2">DNA-(apurinic or apyrimidinic site) lyase</fullName>
            <shortName evidence="2">AP lyase</shortName>
            <ecNumber evidence="2">4.2.99.18</ecNumber>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metig_1278</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">DNA repair enzyme that is part of the base excision repair (BER) pathway; protects from oxidative damage by removing the major product of DNA oxidation, 8-oxoguanine (GO), from single- and double-stranded DNA substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>2'-deoxyribonucleotide-(2'-deoxyribose 5'-phosphate)-2'-deoxyribonucleotide-DNA = a 3'-end 2'-deoxyribonucleotide-(2,3-dehydro-2,3-deoxyribose 5'-phosphate)-DNA + a 5'-end 5'-monophospho-2'-deoxyribonucleoside-DNA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:66592"/>
            <dbReference type="Rhea" id="RHEA-COMP:13180"/>
            <dbReference type="Rhea" id="RHEA-COMP:16897"/>
            <dbReference type="Rhea" id="RHEA-COMP:17067"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:136412"/>
            <dbReference type="ChEBI" id="CHEBI:157695"/>
            <dbReference type="ChEBI" id="CHEBI:167181"/>
            <dbReference type="EC" id="4.2.99.18"/>
        </reaction>
    </comment>
    <comment type="domain">
        <text evidence="2">Contains two alpha-helical subdomains, with the 8-oxoguanine binding site located in a cleft at their interface. Contains a helix-hairpin-helix (HhH) structural motif and a Gly/Pro-rich sequence followed by a conserved Asp (HhH-GPD motif).</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the archaeal N-glycosylase/DNA lyase (AGOG) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.2.2.-" evidence="2"/>
    <dbReference type="EC" id="4.2.99.18" evidence="2"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96815.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799413.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1278"/>
    <dbReference type="EnsemblBacteria" id="AEF96815">
        <property type="protein sequence ID" value="AEF96815"/>
        <property type="gene ID" value="Metig_1278"/>
    </dbReference>
    <dbReference type="GeneID" id="10644141"/>
    <dbReference type="KEGG" id="mig:Metig_1278"/>
    <dbReference type="HOGENOM" id="CLU_085935_0_0_2"/>
    <dbReference type="OMA" id="VKMFGYA"/>
    <dbReference type="OrthoDB" id="108705at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140078">
        <property type="term" value="F:class I DNA-(apurinic or apyrimidinic site) endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000702">
        <property type="term" value="F:oxidized base lesion DNA N-glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01168">
        <property type="entry name" value="AGOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016544">
        <property type="entry name" value="AGOG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015254">
        <property type="entry name" value="AGOG-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011257">
        <property type="entry name" value="DNA_glycosylase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09171">
        <property type="entry name" value="AGOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF008955">
        <property type="entry name" value="AGOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48150">
        <property type="entry name" value="SSF48150"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0227">DNA damage</keyword>
    <keyword evidence="1 2" id="KW-0228">DNA excision</keyword>
    <keyword evidence="2" id="KW-0234">DNA repair</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 4" id="KW-0456">Lyase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="6"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with DNA" evidence="2">
        <location>
            <position position="158"/>
        </location>
    </feature>
    <feature type="active site" evidence="2">
        <location>
            <position position="190"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="38"/>
        </location>
        <ligand>
            <name>8-oxoguanine</name>
            <dbReference type="ChEBI" id="CHEBI:52617"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="66"/>
        </location>
        <ligand>
            <name>8-oxoguanine</name>
            <dbReference type="ChEBI" id="CHEBI:52617"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="77"/>
        </location>
        <ligand>
            <name>8-oxoguanine</name>
            <dbReference type="ChEBI" id="CHEBI:52617"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="162"/>
        </location>
        <ligand>
            <name>8-oxoguanine</name>
            <dbReference type="ChEBI" id="CHEBI:52617"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="188"/>
        </location>
        <ligand>
            <name>8-oxoguanine</name>
            <dbReference type="ChEBI" id="CHEBI:52617"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="224"/>
        </location>
        <ligand>
            <name>8-oxoguanine</name>
            <dbReference type="ChEBI" id="CHEBI:52617"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="228"/>
        </location>
        <ligand>
            <name>8-oxoguanine</name>
            <dbReference type="ChEBI" id="CHEBI:52617"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEF96815.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="257" mass="30658" checksum="8AB5A75BCC077397" modified="2011-07-27" version="1">MNKGKIDKCKEKIEELKSILKELGIDTAKELEENVDLQYLYLKNLQKNLKDDELFIKLVILNALVSYQLSSTGELWWKEFSNYWSEIEIKDDIFKEYKDFLVNCKGNRRLLNAKMKRIDKIEPFLKNLGIGDIKKYYKNMNVFRNHLANQLKTKKESKTIVFTVKMFGYASRISFNKFIPYPMEIEIPKDSRIEKYTRKFTNEEPIKFWKKIGREINLPPLHIDSILWPVLGKSHEVRELLKKHCKKSDLIFKLVEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEP4</accession>
    <name>F6BEP4_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Phosphoribosyl-ATP pyrophosphatase</fullName>
            <shortName evidence="8">PRA-PH</shortName>
            <ecNumber evidence="8">3.6.1.31</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">hisE</name>
        <name evidence="9" type="ordered locus">Metig_1304</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>1-(5-phospho-beta-D-ribosyl)-ATP + H2O = 1-(5-phospho-beta-D-ribosyl)-5'-AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:22828"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:59457"/>
            <dbReference type="ChEBI" id="CHEBI:73183"/>
            <dbReference type="EC" id="3.6.1.31"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 8">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/9.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the PRA-PH family.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.31" evidence="8"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96841.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799438.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1304"/>
    <dbReference type="EnsemblBacteria" id="AEF96841">
        <property type="protein sequence ID" value="AEF96841"/>
        <property type="gene ID" value="Metig_1304"/>
    </dbReference>
    <dbReference type="GeneID" id="10644167"/>
    <dbReference type="KEGG" id="mig:Metig_1304"/>
    <dbReference type="HOGENOM" id="CLU_123337_1_2_2"/>
    <dbReference type="OMA" id="FTHEKGE"/>
    <dbReference type="OrthoDB" id="110768at2157"/>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00007"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004636">
        <property type="term" value="F:phosphoribosyl-ATP diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd11534">
        <property type="entry name" value="NTP-PPase_HisIE_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01020">
        <property type="entry name" value="HisE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008179">
        <property type="entry name" value="HisE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021130">
        <property type="entry name" value="PRib-ATP_PPHydrolase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01503">
        <property type="entry name" value="PRA-PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03188">
        <property type="entry name" value="histidine_hisI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="6 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 8" id="KW-0368">Histidine biosynthesis</keyword>
    <keyword evidence="5 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AEF96841.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="96" mass="11152" checksum="C737CF0BC4ED4767" modified="2011-07-27" version="1">MDVLREVYAIIQQRIKEKPEGSYVAKLTTDDKKKAINKICEKIGEEATELILAAKDNNKDEVIYESADLIFHIMVLLAYLGIEYDELMAEFERRKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>F6BER6</accession>
    <name>F6BER6_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="8 19">Adenosylcobinamide-GDP ribazoletransferase</fullName>
            <ecNumber evidence="7 19">2.7.8.26</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18 19">Cobalamin synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17 19">Cobalamin-5'-phosphate synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="primary">cobS</name>
        <name evidence="20" type="ordered locus">Metig_1326</name>
    </gene>
    <organism evidence="21">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="16 19">Joins adenosylcobinamide-GDP and alpha-ribazole to generate adenosylcobalamin (Ado-cobalamin). Also synthesizes adenosylcobalamin 5'-phosphate from adenosylcobinamide-GDP and alpha-ribazole 5'-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 19">
            <text>adenosylcob(III)inamide-GDP + alpha-ribazole 5'-phosphate = adenosylcob(III)alamin 5'-phosphate + GMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23560"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57918"/>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
            <dbReference type="ChEBI" id="CHEBI:60487"/>
            <dbReference type="ChEBI" id="CHEBI:60493"/>
            <dbReference type="EC" id="2.7.8.26"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 19">
            <text>adenosylcob(III)inamide-GDP + alpha-ribazole = adenosylcob(III)alamin + GMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16049"/>
            <dbReference type="ChEBI" id="CHEBI:10329"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:18408"/>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
            <dbReference type="ChEBI" id="CHEBI:60487"/>
            <dbReference type="EC" id="2.7.8.26"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3 19">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="5 19">Cofactor biosynthesis; adenosylcobalamin biosynthesis; adenosylcobalamin from cob(II)yrinate a,c-diamide: step 7/7.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="19">Cell membrane</location>
            <topology evidence="19">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6 19">Belongs to the CobS family.</text>
    </comment>
    <dbReference type="EC" id="2.7.8.26" evidence="7 19"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96863.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1326"/>
    <dbReference type="EnsemblBacteria" id="AEF96863">
        <property type="protein sequence ID" value="AEF96863"/>
        <property type="gene ID" value="Metig_1326"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_1326"/>
    <dbReference type="HOGENOM" id="CLU_057426_2_0_2"/>
    <dbReference type="OMA" id="FMDSMDG"/>
    <dbReference type="UniPathway" id="UPA00148">
        <property type="reaction ID" value="UER00238"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051073">
        <property type="term" value="F:adenosylcobinamide-GDP ribazoletransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008818">
        <property type="term" value="F:cobalamin 5'-phosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00719">
        <property type="entry name" value="CobS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003805">
        <property type="entry name" value="CobS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34148">
        <property type="entry name" value="PTHR34148"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02654">
        <property type="entry name" value="CobS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00317">
        <property type="entry name" value="cobS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 19" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10 19" id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword evidence="13 19" id="KW-0460">Magnesium</keyword>
    <keyword evidence="15 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 19" id="KW-0808">Transferase</keyword>
    <keyword evidence="12 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14 19" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="47"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="71"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="121"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="190"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="239"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00010561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00013200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00015850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00025228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00032605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00032853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="AEF96863.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="275" mass="30635" checksum="1569A196F98EE0DC" modified="2011-07-27" version="1">MVLGMVDFQYKNTKSETMIEDIRNLIAFMTRIPIGTENFDFEKIAEYFFIIPIIGLIIGGFGALLAYILSYIFPSTIVGIIVMFFLLYVQGFHHVDGLADFGDAWMLMGSPKRKLEVMKDVYMGIGGLIFVFFVELISIFAIIYAIEFSVVYILIAEICSRLGMLACACCGTPCSEGTGRYFVKKSDEKFIVMGLMLTLLIVFPLAGKLGVLCVILAILTGGYIARVANKHFRCVNGDVLGATNEITRMVVLLGIVFYLYANQSLQPLHNLIQMI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>F6BES1</accession>
    <name>F6BES1_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ribose-phosphate pyrophosphokinase</fullName>
            <shortName evidence="2">RPPK</shortName>
            <ecNumber evidence="2">2.7.6.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">5-phospho-D-ribosyl alpha-1-diphosphate</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="2">Phosphoribosyl diphosphate synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="2">Phosphoribosyl pyrophosphate synthase</fullName>
            <shortName evidence="2">P-Rib-PP synthase</shortName>
            <shortName evidence="2">PRPP synthase</shortName>
            <shortName evidence="2">PRPPase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2" type="primary">prs</name>
        <name evidence="6" type="ordered locus">Metig_1331</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Involved in the biosynthesis of the central metabolite phospho-alpha-D-ribosyl-1-pyrophosphate (PRPP) via the transfer of pyrophosphoryl group from ATP to 1-hydroxyl of ribose-5-phosphate (Rib-5-P).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>ATP + D-ribose 5-phosphate = 5-phospho-alpha-D-ribose 1-diphosphate + AMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15609"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.7.6.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="2">Binds 2 Mg(2+) ions per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Metabolic intermediate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate from D-ribose 5-phosphate (route I): step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ribose-phosphate pyrophosphokinase family. Class III (archaeal) subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.6.1" evidence="2"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96868.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1331"/>
    <dbReference type="EnsemblBacteria" id="AEF96868">
        <property type="protein sequence ID" value="AEF96868"/>
        <property type="gene ID" value="Metig_1331"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_1331"/>
    <dbReference type="HOGENOM" id="CLU_033546_2_2_2"/>
    <dbReference type="OMA" id="FGWARQD"/>
    <dbReference type="UniPathway" id="UPA00087">
        <property type="reaction ID" value="UER00172"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004749">
        <property type="term" value="F:ribose phosphate diphosphokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006015">
        <property type="term" value="P:5-phosphoribose 1-diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009165">
        <property type="term" value="P:nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009156">
        <property type="term" value="P:ribonucleoside monophosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00583_A">
        <property type="entry name" value="RibP_PPkinase_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029099">
        <property type="entry name" value="Pribosyltran_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRibTrfase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005946">
        <property type="entry name" value="Rib-P_diPkinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037514">
        <property type="entry name" value="Rib-P_diPkinase_arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10210">
        <property type="entry name" value="PTHR10210"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00156">
        <property type="entry name" value="Pribosyltran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13793">
        <property type="entry name" value="Pribosyltran_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="SSF53271"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01251">
        <property type="entry name" value="ribP_PPkin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 6" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1 2 3" id="KW-0545">Nucleotide biosynthesis</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Pribosyltran_N" evidence="5">
        <location>
            <begin position="1"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="domain" description="Pribosyltran" evidence="4">
        <location>
            <begin position="131"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="region of interest" description="Ribose-5-phosphate binding" evidence="2">
        <location>
            <begin position="216"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="active site" evidence="2">
        <location>
            <position position="186"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="34"/>
            <end position="36"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="92"/>
            <end position="93"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="125"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="163"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="188"/>
        </location>
        <ligand>
            <name>D-ribose 5-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="212"/>
        </location>
        <ligand>
            <name>D-ribose 5-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU004324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF13793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEF96868.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="285" mass="31350" checksum="E392DF2B8638AD66" modified="2011-07-27" version="1">MIIIPGSNSQDLAFRVAKLTNSKLARVESKRFPDGEIYVRVHDDVKDEDVVIIQTQNRQNDGIIETILLCDALRDEGANSITVVAPYLAYARQDKKFNPGEPISIRALAKIYSDICDKLITINPHETHIERFFEIPFIYGDAVPKLAEYVKDKLNNPIALAPDKGAIEFAKTAAEILNCEYDYLEKTRISPTEIQIAPKNLDAEGRDVLIVDDIISTGGTMATAIKLLKEQGAKKIIAACVHPVLIGDALNKLYAGGADEVVGTDTFLSEVSKVSVDDVIAKLLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>F6BES2</accession>
    <name>F6BES2_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Hydrogenase maturation protease HycI</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1332</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96869.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1332"/>
    <dbReference type="MEROPS" id="A31.003"/>
    <dbReference type="EnsemblBacteria" id="AEF96869">
        <property type="protein sequence ID" value="AEF96869"/>
        <property type="gene ID" value="Metig_1332"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_1332"/>
    <dbReference type="HOGENOM" id="CLU_099037_4_1_2"/>
    <dbReference type="OMA" id="EMCAANP"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008047">
        <property type="term" value="F:enzyme activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06067">
        <property type="entry name" value="H2MP_MemB-H2evol"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004420">
        <property type="entry name" value="Pept_A31_hyd_mat_HycI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023430">
        <property type="entry name" value="Pept_HybD-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000671">
        <property type="entry name" value="Peptidase_A31"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30302">
        <property type="entry name" value="PTHR30302"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01750">
        <property type="entry name" value="HycI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00446">
        <property type="entry name" value="HYDRGNUPTAKE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53163">
        <property type="entry name" value="SSF53163"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00142">
        <property type="entry name" value="hycI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00072">
        <property type="entry name" value="hydrog_prot"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96869.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="208" mass="23430" checksum="4222C30A9C5FDF8D" modified="2011-07-27" version="1">MLFKILRRHIIKLKISHIIIFHLIVAINFLGVVMEALGLKEFLENCEKLVIMGIGNEMKGDDGVGIYIVKEIAKRFGKDVNDINSEVIKISENIILLNCGTVPENFTDVLKRESPTHIIMVDAALMNEEIGTIKVINPEDIADVGFSTHALPLSIIVKYIKKSINTKIIVIGIEPKIIDFDKPLSDEVRRCADNFVEYLIKIINTIFC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>F6BET1</accession>
    <name>F6BET1_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CRISPR system Cms protein Csm4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Metig_1343</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CRISPR-associated Csm4 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96878.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1343"/>
    <dbReference type="EnsemblBacteria" id="AEF96878">
        <property type="protein sequence ID" value="AEF96878"/>
        <property type="gene ID" value="Metig_1343"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_1343"/>
    <dbReference type="HOGENOM" id="CLU_062371_0_1_2"/>
    <dbReference type="OMA" id="SAYFIVE"/>
    <dbReference type="OrthoDB" id="69746at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005510">
        <property type="entry name" value="Csm4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040932">
        <property type="entry name" value="Csm4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005537">
        <property type="entry name" value="RAMP_III_fam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17953">
        <property type="entry name" value="Csm4_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03787">
        <property type="entry name" value="RAMPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01903">
        <property type="entry name" value="cas5_csm4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0051">Antiviral defense</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Csm4_C" evidence="5">
        <location>
            <begin position="223"/>
            <end position="310"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00016109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF17953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEF96878.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="314" mass="36415" checksum="ADF24FAD10C39F56" modified="2011-07-27" version="1">MVKIMQMKAIKLYFQTPLYMGGERGAIIPVHSDTIFGAIINALIYLNVDIEPFVGAFEENRFSISSAFPFRGEKLFFPKPFHINSYINKKIDKDPNLEGYLKLKKFKKKKFFDKENFEKMINGKIPDLDEINYEEKYGYKVADIPKIVLDRITVNSHLYHISQVFFEDNAGLYFLFQGDDEIFKNYIKPAIRLLGDEGIGGKRTWGLGKFKPKFEDIALKTQESDLFVTLSLTIPKNKNVLHLWDFIKRGDWIFTRNGKPRRKPTIMMVKEGSILNKDPGEIIDLDNYGNFSGEIGHKVLVNAKSFLIPVRWKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEU9</accession>
    <name>F6BEU9_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Pyrrolo-quinoline quinone repeat-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0124</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95685.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798294.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0124"/>
    <dbReference type="EnsemblBacteria" id="AEF95685">
        <property type="protein sequence ID" value="AEF95685"/>
        <property type="gene ID" value="Metig_0124"/>
    </dbReference>
    <dbReference type="GeneID" id="25395051"/>
    <dbReference type="KEGG" id="mig:Metig_0124"/>
    <dbReference type="HOGENOM" id="CLU_407501_0_0_2"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018391">
        <property type="entry name" value="PQQ_beta_propeller_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002372">
        <property type="entry name" value="PQQ_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_supfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13360">
        <property type="entry name" value="PQQ_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00564">
        <property type="entry name" value="PQQ"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="SSF48452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="SSF50998"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="13"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="59"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="105"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="197"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF95685.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="674" mass="77558" checksum="08EAF6BD9ED1E799" modified="2011-07-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEV0</accession>
    <name>F6BEV0_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Fe(3+)-transporting ATPase</fullName>
            <ecNumber evidence="5">3.6.3.30</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Metig_0125</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.6.3.30" evidence="5"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95686.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798295.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0125"/>
    <dbReference type="EnsemblBacteria" id="AEF95686">
        <property type="protein sequence ID" value="AEF95686"/>
        <property type="gene ID" value="Metig_0125"/>
    </dbReference>
    <dbReference type="GeneID" id="10642960"/>
    <dbReference type="KEGG" id="mig:Metig_0125"/>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_2"/>
    <dbReference type="OMA" id="IMMQKIM"/>
    <dbReference type="OrthoDB" id="55756at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="2"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="34"/>
            <end position="41"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEF95686.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="236" mass="26034" checksum="DDC0F75BEF98880B" modified="2011-07-27" version="1">MLKVNKLHAGYGKLEIVHGISLHVNEEEIVTIIGPNGCGKSTFLKSIFGLTTIMDGEIIFNNKNITNMRPDLIAREGIGYVPQLDNVFPNLTVEENLEMGGYILPKSQLKERMEEVYDFFEVLREKKDVLAGSLSGGERQMLAMARALMAKPKLLLLDEPTAGLSPKMVSTVLKKIREITDMGVGILLVEQNAKKALEISDRGYVFAGGVVVYEGKASDILNHKEIGELYLGKKAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEV1</accession>
    <name>F6BEV1_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Ribosomal-protein-alanine acetyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metig_0127</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95687.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0127"/>
    <dbReference type="EnsemblBacteria" id="AEF95687">
        <property type="protein sequence ID" value="AEF95687"/>
        <property type="gene ID" value="Metig_0127"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0127"/>
    <dbReference type="HOGENOM" id="CLU_013985_23_0_2"/>
    <dbReference type="OMA" id="SGEIMGY"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031415">
        <property type="term" value="C:NatA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004596">
        <property type="term" value="F:peptide alpha-N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006474">
        <property type="term" value="P:N-terminal protein amino acid acetylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006464">
        <property type="entry name" value="AcTrfase_RimI/Ard1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045047">
        <property type="entry name" value="Ard1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23091">
        <property type="entry name" value="PTHR23091"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01575">
        <property type="entry name" value="rimI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="3">
        <location>
            <begin position="2"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEF95687.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="159" mass="19089" checksum="F7BDCD779936D832" modified="2011-07-27" version="1">MIRIRKFKLEDLDRIEEIEKQSFKKTYPRFLLTHLYTNFPDGFIIAEINNKIVGYAIGTIEWGNGHIVSIAVDREFRNRGIGTALIEHLERYFFERCNVKYIVLEVRVSNKTARMFYYKRGYVDKRFLPNYYDDGEDAILMIKKRKNLETNYPIIVSMW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-06-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEX6</accession>
    <name>F6BEX6_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_0154</name>
        <name evidence="2" type="ordered locus">Metig_0616</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95712.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96167.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798321.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF95712">
        <property type="protein sequence ID" value="AEF95712"/>
        <property type="gene ID" value="Metig_0154"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96167">
        <property type="protein sequence ID" value="AEF96167"/>
        <property type="gene ID" value="Metig_0616"/>
    </dbReference>
    <dbReference type="GeneID" id="10643455"/>
    <dbReference type="KEGG" id="mig:Metig_0154"/>
    <dbReference type="KEGG" id="mig:Metig_0616"/>
    <dbReference type="HOGENOM" id="CLU_2802249_0_0_2"/>
    <dbReference type="OrthoDB" id="4247at2182"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF95712.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96167.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="67" mass="8058" checksum="12C2C488A9882B97" modified="2011-07-27" version="1">MDAVKIVITLIDKYLTDKIEQPKRRGPKGYDLVLKLRLLAYGVLKGLYYTRELVRYLKKYYKKPDYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-06-02" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEZ0</accession>
    <name>F6BEZ0_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0169</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95726.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798335.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF95726">
        <property type="protein sequence ID" value="AEF95726"/>
        <property type="gene ID" value="Metig_0169"/>
    </dbReference>
    <dbReference type="GeneID" id="10643004"/>
    <dbReference type="KEGG" id="mig:Metig_0169"/>
    <dbReference type="HOGENOM" id="CLU_181980_0_0_2"/>
    <dbReference type="OMA" id="HAWDLYR"/>
    <dbReference type="OrthoDB" id="126390at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="67"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF95726.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="103" mass="12155" checksum="95736A0A83199D35" modified="2011-07-27" version="1">MFGRGWFGRGRGFWRHYIPSTVGGRYRYIGPCRCGMGPHAYYEDESGRIVHAWDLYRVPIATSTTDRTYLVDRLKELEEEKAIIEDEIAEIKKRLNEIEKQNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>F6BF14</accession>
    <name>F6BF14_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 5">RNA 3'-terminal phosphate cyclase</fullName>
            <shortName evidence="5">RNA cyclase</shortName>
            <shortName evidence="5">RNA-3'-phosphate cyclase</shortName>
            <ecNumber evidence="5">6.5.1.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">rtcA</name>
        <name evidence="8" type="ordered locus">Metig_0193</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the conversion of 3'-phosphate to a 2',3'-cyclic phosphodiester at the end of RNA. The mechanism of action of the enzyme occurs in 3 steps: (A) adenylation of the enzyme by ATP; (B) transfer of adenylate to an RNA-N3'P to produce RNA-N3'PP5'A; (C) and attack of the adjacent 2'-hydroxyl on the 3'-phosphorus in the diester linkage to produce the cyclic end product. The biological role of this enzyme is unknown but it is likely to function in some aspects of cellular RNA processing.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>a 3'-end 3'-phospho-ribonucleotide-RNA + ATP = a 3'-end 2',3'-cyclophospho-ribonucleotide-RNA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:23976"/>
            <dbReference type="Rhea" id="RHEA-COMP:10463"/>
            <dbReference type="Rhea" id="RHEA-COMP:10464"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:83062"/>
            <dbReference type="ChEBI" id="CHEBI:83064"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.5.1.4"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the RNA 3'-terminal cyclase family. Type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.5.1.4" evidence="5"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95750.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798359.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0193"/>
    <dbReference type="EnsemblBacteria" id="AEF95750">
        <property type="protein sequence ID" value="AEF95750"/>
        <property type="gene ID" value="Metig_0193"/>
    </dbReference>
    <dbReference type="GeneID" id="10643028"/>
    <dbReference type="KEGG" id="mig:Metig_0193"/>
    <dbReference type="HOGENOM" id="CLU_027882_0_0_2"/>
    <dbReference type="OMA" id="PKPGLSH"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003963">
        <property type="term" value="F:RNA-3'-phosphate cyclase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.65.10.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00200">
        <property type="entry name" value="RTC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013791">
        <property type="entry name" value="RNA3'-term_phos_cycl_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023797">
        <property type="entry name" value="RNA3'_phos_cyclase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037136">
        <property type="entry name" value="RNA3'_phos_cyclase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000228">
        <property type="entry name" value="RNA3'_term_phos_cyc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017770">
        <property type="entry name" value="RNA3'_term_phos_cyc_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020719">
        <property type="entry name" value="RNA3'_term_phos_cycl-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013792">
        <property type="entry name" value="RNA3'P_cycl/enolpyr_Trfase_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036553">
        <property type="entry name" value="RPTC_insert"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11096">
        <property type="entry name" value="PTHR11096"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11096:SF0">
        <property type="entry name" value="PTHR11096:SF0"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01137">
        <property type="entry name" value="RTC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05189">
        <property type="entry name" value="RTC_insert"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005378">
        <property type="entry name" value="RNA3'_term_phos_cycl_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52913">
        <property type="entry name" value="SSF52913"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55205">
        <property type="entry name" value="SSF55205"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03399">
        <property type="entry name" value="RNA_3prim_cycl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01287">
        <property type="entry name" value="RTC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 5 8" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RTC" evidence="6">
        <location>
            <begin position="8"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="domain" description="RTC_insert" evidence="7">
        <location>
            <begin position="185"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="active site" description="Tele-AMP-histidine intermediate" evidence="5">
        <location>
            <position position="302"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="100"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="278"/>
            <end position="282"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00021428"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF05189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEF95750.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="337" mass="36825" checksum="EB3AB3D58F5F5C4A" modified="2011-07-27" version="1">MIVIDGSYLEGGGQIVRTSIALSAITQKPVKIINIRKKRKNKGLSYQHIACVKAVKKLCNADVDGLYVGSEELTFYPNKISPKSFKLDIGTAGSISLVIQTLLPISAVIKKPTTITITGGTDVKNAPPIDYMKNVVLKVLSCVGVDANIEVLKRGFYPEGGGVVKLTLKPSKIKEIELIEHSEDKKVEGIAYAQNLTDDIPKRMRKSCSELLMKNGYLPNIKLENSKGISTGAGIVLWCDTVGESCLGEKGLRSEIVGKMAAERLLEELKSGMALDKYMGDQIIPFLAMGKGRVGVSKITNHTRTNIYVVENFFDVKFEIEEYNENGCNGHIIKTEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>F6BF83</accession>
    <name>F6BF83_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Oxidoreductase/nitrogenase component 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_1418</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96953.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799549.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1418"/>
    <dbReference type="EnsemblBacteria" id="AEF96953">
        <property type="protein sequence ID" value="AEF96953"/>
        <property type="gene ID" value="Metig_1418"/>
    </dbReference>
    <dbReference type="GeneID" id="10644291"/>
    <dbReference type="KEGG" id="mig:Metig_1418"/>
    <dbReference type="HOGENOM" id="CLU_025876_1_1_2"/>
    <dbReference type="OMA" id="NICKLSY"/>
    <dbReference type="OrthoDB" id="14587at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000510">
        <property type="entry name" value="Nase/OxRdtase_comp1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00148">
        <property type="entry name" value="Oxidored_nitro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Oxidored_nitro" evidence="2">
        <location>
            <begin position="27"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="269"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF96953.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="467" mass="53471" checksum="42496220604C4664" modified="2011-07-27" version="1">MQYKEQKIRICSNLPLHRQSGIPGKVCGALRIANQIKGCIPILHSPVGCAFQRKINTFAPYEITYNLPCTNLTEVDTVYGGHEKLVEKILEVNKKYHPELILIISTCAPDLIGEDYDITLDEVRDMIDAKIIYDTGNAKRAPVGMQSVLYSLATQVMEEQDKIEKSLNICKLSYHRTDLVDEFVDILKEMGAHVNGVYFNNNTVEDIKNIPRAELNLVDFPADWVLYVEKKFGTKYLVPPRLKIEDSLTTLNGFAKYLYAIADILDLDTNVIEKRKKEADEKLEKYRNKLKGKKIASGFSVHGGAMQTLIEDVGMECPVLILKTRRMSLMMKENAIKHISNSMIRFIENHQGYAPEVLINPTTEEEIKAMKEHGIELCVGGMENNMFEYLRNGIKLFDMRRMMQISKMGFSNSINIAREAYKIISKEPKSHLLLGNIDYSMKYPSLSNYWANIVDIFQTCWYCDYKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>F6BF85</accession>
    <name>F6BF85_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Nucleoside recognition domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_1420</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96955.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799551.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1420"/>
    <dbReference type="EnsemblBacteria" id="AEF96955">
        <property type="protein sequence ID" value="AEF96955"/>
        <property type="gene ID" value="Metig_1420"/>
    </dbReference>
    <dbReference type="GeneID" id="10644293"/>
    <dbReference type="KEGG" id="mig:Metig_1420"/>
    <dbReference type="HOGENOM" id="CLU_048086_2_0_2"/>
    <dbReference type="OMA" id="HQFPYYA"/>
    <dbReference type="OrthoDB" id="59004at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011642">
        <property type="entry name" value="Gate_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038880">
        <property type="entry name" value="MJ0871-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38139">
        <property type="entry name" value="PTHR38139"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07670">
        <property type="entry name" value="Gate"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="83"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="116"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="178"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="218"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="251"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="287"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="domain" description="Gate" evidence="2">
        <location>
            <begin position="16"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="domain" description="Gate" evidence="2">
        <location>
            <begin position="175"/>
            <end position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF96955.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="308" mass="34232" checksum="E629C78222D559AC" modified="2011-07-27" version="1">MDVLSYLTQTILLSSVGIIIAGIIEETNLLSIIKKITKPLCLISNLPEECVVALLGNFINPTVGKSMLAKFYKENKINSRETIVTTIISPLPIILGESIFRVQLPLAIVLLGYKLGTIYVLFNMLSGFLMALIGIIYSNIAFERKFLNIDTNNNEKIIFNRAVITKGIKKSIKLLKKVIPMIVIFTLLIHCLMTFGLIDIIKEIFTPIFSILDLPGEAVMVLVANFAHFSAGYATVSILIKNGVLNEKQALLTLLIGNIIGITMIYLKHSIGTYVALFGRFGLKLAMINYTISIIIKIFLIMLIMLMF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>F6BA83</accession>
    <name>F6BA83_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="6">NADH-ubiquinone oxidoreductase chain 4L</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Metig_0216</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95773.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798382.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0216"/>
    <dbReference type="EnsemblBacteria" id="AEF95773">
        <property type="protein sequence ID" value="AEF95773"/>
        <property type="gene ID" value="Metig_0216"/>
    </dbReference>
    <dbReference type="GeneID" id="10643051"/>
    <dbReference type="KEGG" id="mig:Metig_0216"/>
    <dbReference type="HOGENOM" id="CLU_082058_2_1_2"/>
    <dbReference type="OMA" id="YTIGFKR"/>
    <dbReference type="OrthoDB" id="108209at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039428">
        <property type="entry name" value="NUOK/Mnh_C1-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00420">
        <property type="entry name" value="Oxidored_q2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0830">Ubiquinone</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="32"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="79"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEF95773.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="118" mass="12707" checksum="6BDEE5234BEA3D9C" modified="2011-07-27" version="1">MELQVASFITAGLLIVIGLYGVFFVDNVIKKIIALSAMGNGVNLVLIAMGYNGGVVPIKLPNMPFEVFASKSAYPLPQALVLTNIVIEASMLAIMLALSMVLYKKYKTLKASVILKED</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-09-29" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>F6BA96</accession>
    <name>F6BA96_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="3">AAA ATPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_0229</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the archaeal ATPase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95786.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798395.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF95786">
        <property type="protein sequence ID" value="AEF95786"/>
        <property type="gene ID" value="Metig_0229"/>
    </dbReference>
    <dbReference type="GeneID" id="10643064"/>
    <dbReference type="KEGG" id="mig:Metig_0229"/>
    <dbReference type="HOGENOM" id="CLU_068608_0_0_2"/>
    <dbReference type="OMA" id="YVIEECK"/>
    <dbReference type="OrthoDB" id="50712at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011579">
        <property type="entry name" value="ATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01637">
        <property type="entry name" value="ATPase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA" evidence="2">
        <location>
            <begin position="21"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF95786.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="359" mass="42378" checksum="89A5A69A89EB4AD1" modified="2011-07-27" version="1">MDLFNREKEINEIMSIIESEPNFIYFLYGPINSGKTALINEIINNRLDKDKYVVFYFDLREIFISKYDDFIEVLFEEYNENKKPVEIIKGLIKDTPLLFGVPVPKNTLEELLNKKTTKNVFRYITNILLEIKKSGKIPIIILDELQKIGDLKINGFLIYELFNYFISLTKHKHLCHVFCLSSDSLFIEKVYSEAILNGRAKYLLVDDFDKETAIKFMDFLSKRILNKKLSNEEKELIYSYVGGKSIDIIYIIQELKNKNLEEILDYLLKDAIQKLDMFLEILNYSKPKIEIGNEIIELKKEDIINALRLFKEKYMINKKEIPTPVYMYLVKKNILFLNPVEGTLRPQSFLIWNGIKRLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAA9</accession>
    <name>F6BAA9_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Methyltransferase type 12</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_0242</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95799.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798408.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF95799">
        <property type="protein sequence ID" value="AEF95799"/>
        <property type="gene ID" value="Metig_0242"/>
    </dbReference>
    <dbReference type="GeneID" id="10643077"/>
    <dbReference type="KEGG" id="mig:Metig_0242"/>
    <dbReference type="HOGENOM" id="CLU_1331419_0_0_2"/>
    <dbReference type="OMA" id="QHYWEIG"/>
    <dbReference type="OrthoDB" id="37084at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF95799.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="207" mass="24774" checksum="3EC87306B859712F" modified="2011-07-27" version="1">MKFNPQVPHEHYINNYDKKHRWISYWYQINEVISTKPQKVLEIGPGNGTISNYLKKICKINVTTVDIDKNLNPDYVCSVTELKNIFKKDSFDTVLCAQVLEHLPFRYFEESLKNIQYVTKNYAIITLPHYGINISFKIKILFGEFNFVLCIPYPKEHKFDGQHYWEIGKKGYSLRRIEKIISKYFIILKNYLIPEHPYHRVFVLKKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAC4</accession>
    <name>F6BAC4_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Queuosine synthesis-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_0258</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95814.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798423.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0258"/>
    <dbReference type="EnsemblBacteria" id="AEF95814">
        <property type="protein sequence ID" value="AEF95814"/>
        <property type="gene ID" value="Metig_0258"/>
    </dbReference>
    <dbReference type="GeneID" id="10643093"/>
    <dbReference type="KEGG" id="mig:Metig_0258"/>
    <dbReference type="HOGENOM" id="CLU_064237_0_0_2"/>
    <dbReference type="OMA" id="PYRMYEH"/>
    <dbReference type="OrthoDB" id="54396at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012122">
        <property type="entry name" value="ATPase_PP-loop_MJ1016"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018317">
        <property type="entry name" value="QueC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06508">
        <property type="entry name" value="QueC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036670">
        <property type="entry name" value="ATPase_UCP036670"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF95814.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="285" mass="33258" checksum="2BDD720AAB1171E4" modified="2011-07-27" version="1">MEFSEWTKEKRKLNNFEELKEDILNNFKEQGLEDEKIVVMLSGGKDSSTALALAKDLGLNVHLCVHFVHKWSWELAKNEAERIAKKFNVPILFYDITDELRRRAIGAKGSSICRICKNIMKDKVVDIAREHGAKIIMTGDTALEKISGPIMDYLREKYGKVVYKKMELTPVPKKYKMMFFRPLIRLAHEDVLKLKEYYGIDIKRVHEVGDKIGFWREGCPMQYCDEDTIITEELLDELYELNKKITDIAREHGFRASIKLPSKKIIVVPKKKEYIDMIKKTLNIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAD1</accession>
    <name>F6BAD1_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="1 2">GTP cyclohydrolase III</fullName>
            <ecNumber evidence="1 2">3.5.4.29</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">gch3</name>
        <name evidence="3" type="ordered locus">Metig_0265</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 2">Catalyzes the formation of 2-amino-5-formylamino-6-ribofuranosylamino-4(3H)-pyrimidinone ribonucleotide monophosphate and inorganic phosphate from GTP. Also has an independent pyrophosphate phosphohydrolase activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 2">
            <text>GTP + 3 H2O = 2-amino-5-formylamino-6-(5-phospho-D-ribosylamino)pyrimidin-4(3H)-one + 2 H(+) + 2 phosphate</text>
            <dbReference type="Rhea" id="RHEA:22468"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57258"/>
            <dbReference type="EC" id="3.5.4.29"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the archaeal-type GTP cyclohydrolase family.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.29" evidence="1 2"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95821.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798430.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0265"/>
    <dbReference type="EnsemblBacteria" id="AEF95821">
        <property type="protein sequence ID" value="AEF95821"/>
        <property type="gene ID" value="Metig_0265"/>
    </dbReference>
    <dbReference type="GeneID" id="10643100"/>
    <dbReference type="KEGG" id="mig:Metig_0265"/>
    <dbReference type="HOGENOM" id="CLU_080076_0_0_2"/>
    <dbReference type="OMA" id="VQIAHID"/>
    <dbReference type="OrthoDB" id="56576at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043740">
        <property type="term" value="F:GTP cyclohydrolase IIa activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009058">
        <property type="term" value="P:biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00608">
        <property type="entry name" value="GTP_cyclohydro_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007839">
        <property type="entry name" value="GTP_CycHdrlase_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42202">
        <property type="entry name" value="PTHR42202"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05165">
        <property type="entry name" value="GCH_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF009265">
        <property type="entry name" value="GTP_cyclohydro_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1 2 3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00608"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRNR" id="PIRNR009265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF95821.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="270" mass="30394" checksum="F78D18CF33F59B56" modified="2011-07-27" version="1">MIQITVIQIDNYGPWTVTPHPRRESDLQALQARLYADLNLMFGMHKGLVFYTRFDNLIAITNGIDLLTHQKIQDSIKNRYPITISMVIASAETPYKAQKLATKKLQEHGSAQDESRREVLDVANELVMDGYVQLAHIDINNITERLTDLESAYDTYINVNKAKLMLMEELKKYDAMLFFIGGDNFMCPCNGMSEDDFLNVFEKIKEKCGVELKAGIGIGKTAEDASNLADIGLELIRAGKTGRQVCTLKQEGCEVLSSITTKTTNYNRLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAH2</accession>
    <name>F6BAH2_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Beta-ribofuranosylaminobenzene 5'-phosphate synthase</fullName>
            <shortName evidence="1">Beta-RFA-P synthase</shortName>
            <ecNumber evidence="1">2.4.2.54</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metig_1450</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the condensation of 4-aminobenzoate (pABA) with 5-phospho-alpha-D-ribose 1-diphosphate (PRPP) to produce beta-ribofuranosylaminobenzene 5'-phosphate (beta-RFA-P).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>4-hydroxybenzoate + 5-phospho-alpha-D-ribose 1-diphosphate + H(+) = 4-(beta-D-ribofuranosyl)phenol 5'-phosphate + CO2 + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:48556"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:17879"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:82767"/>
            <dbReference type="EC" id="2.4.2.54"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; 5,6,7,8-tetrahydromethanopterin biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the beta-RFA-P synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.54" evidence="1"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96985.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799581.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1450"/>
    <dbReference type="EnsemblBacteria" id="AEF96985">
        <property type="protein sequence ID" value="AEF96985"/>
        <property type="gene ID" value="Metig_1450"/>
    </dbReference>
    <dbReference type="GeneID" id="10644323"/>
    <dbReference type="KEGG" id="mig:Metig_1450"/>
    <dbReference type="HOGENOM" id="CLU_061764_0_0_2"/>
    <dbReference type="OMA" id="WGPTFYG"/>
    <dbReference type="OrthoDB" id="47243at2157"/>
    <dbReference type="UniPathway" id="UPA00065"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013750">
        <property type="entry name" value="GHMP_kinase_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006204">
        <property type="entry name" value="GHMP_kinase_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004422">
        <property type="entry name" value="RFAP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20861:SF6">
        <property type="entry name" value="PTHR20861:SF6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08544">
        <property type="entry name" value="GHMP_kinases_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00288">
        <property type="entry name" value="GHMP_kinases_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004884">
        <property type="entry name" value="Sugar_kin_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00144">
        <property type="entry name" value="beta_RFAP_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GHMP_kinases_N" evidence="2">
        <location>
            <begin position="88"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="domain" description="GHMP_kinases_C" evidence="3">
        <location>
            <begin position="231"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRNR" id="PIRNR004884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF08544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEF96985.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="333" mass="36533" checksum="5D4CBF5B3FEDD88E" modified="2011-07-27" version="1">MEIVSPSRIHMGLIDLNGSIGRVDGGVGLALENPNLIIEGKESEEIEIEFDKSILKNFNENYIKDVERRVKDAAEKILKHINEDGVKLLIKKSFPSHSGLGSGTQIALSTGKLVSLIYGKELNAYEIAKITGRGGTSGIGIGAFEMGGFLVDGGHSFGKGKDKEDFRPSSASKGVKPAPIIFRHDFNWDVVLIIPKGEHVYGNKEVDIFKKYCPVPLNEVQRICHLILMKMIPAVIEDDLNAFGEVVNELQTLGFKKVEVSLQSDIVKELIEELQRVGYAGLSSFGPTIYALGNVDLITEKANEILDKHGVDGEIIVTKANNVGYRVKYNINK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAH3</accession>
    <name>F6BAH3_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Phosphoribosyl-AMP cyclohydrolase</fullName>
            <shortName evidence="7">PRA-CH</shortName>
            <ecNumber evidence="7">3.5.4.19</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">hisI</name>
        <name evidence="9" type="ordered locus">Metig_1451</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the hydrolysis of the adenine ring of phosphoribosyl-AMP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>1-(5-phospho-beta-D-ribosyl)-5'-AMP + H2O = 1-(5-phospho-beta-D-ribosyl)-5-[(5-phospho-beta-D-ribosylamino)methylideneamino]imidazole-4-carboxamide</text>
            <dbReference type="Rhea" id="RHEA:20049"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:58435"/>
            <dbReference type="ChEBI" id="CHEBI:59457"/>
            <dbReference type="EC" id="3.5.4.19"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="7">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="7">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 7">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 3/9.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the PRA-CH family.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.19" evidence="7"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96986.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799582.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1451"/>
    <dbReference type="EnsemblBacteria" id="AEF96986">
        <property type="protein sequence ID" value="AEF96986"/>
        <property type="gene ID" value="Metig_1451"/>
    </dbReference>
    <dbReference type="GeneID" id="10644324"/>
    <dbReference type="KEGG" id="mig:Metig_1451"/>
    <dbReference type="HOGENOM" id="CLU_048577_5_3_2"/>
    <dbReference type="OMA" id="TGYRSCF"/>
    <dbReference type="OrthoDB" id="101275at2157"/>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00008"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004635">
        <property type="term" value="F:phosphoribosyl-AMP cyclohydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.810">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01021">
        <property type="entry name" value="HisI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026660">
        <property type="entry name" value="PRA-CH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002496">
        <property type="entry name" value="PRib_AMP_CycHydrolase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038019">
        <property type="entry name" value="PRib_AMP_CycHydrolase_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01502">
        <property type="entry name" value="PRA-CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141734">
        <property type="entry name" value="SSF141734"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="3 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 7" id="KW-0368">Histidine biosynthesis</keyword>
    <keyword evidence="5 7 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="PRA-CH" evidence="8">
        <location>
            <begin position="38"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="85"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="86"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="87"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="89"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="102"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="109"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AEF96986.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="125" mass="14437" checksum="7B739FBC8CF1CCFE" modified="2011-07-27" version="1">MSVDVDEIIKKLNPKFRNIEGKKLLIAIAVDEDKNVLMTAFMSEESLRKTLETGLMHYYSTSRDKIWMKGEESGNIQKVKEIYRDCDGDALLFVVEQKGVACHEGYYSCFHRKLEDGDIVIIDRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAK0</accession>
    <name>F6BAK0_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_1479</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97013.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799608.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1479"/>
    <dbReference type="EnsemblBacteria" id="AEF97013">
        <property type="protein sequence ID" value="AEF97013"/>
        <property type="gene ID" value="Metig_1479"/>
    </dbReference>
    <dbReference type="GeneID" id="10644352"/>
    <dbReference type="KEGG" id="mig:Metig_1479"/>
    <dbReference type="HOGENOM" id="CLU_097039_4_2_2"/>
    <dbReference type="OMA" id="HIHTFFM"/>
    <dbReference type="OrthoDB" id="127880at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.1140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003795">
        <property type="entry name" value="DUF192"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038695">
        <property type="entry name" value="Saro_0823-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37953">
        <property type="entry name" value="PTHR37953"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02643">
        <property type="entry name" value="DUF192"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF97013.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="115" mass="13874" checksum="A1625750C6D49DEB" modified="2011-07-27" version="1">MTNKKIKKVKLDDKEYKIILADNFLKRAFGLMFKDIKDNEAMLFLYKNRKLHIHTFFMKYPIDVIFLKDNRVVEVVKNLKPWRTYKCKNYSNAMLEMKSGNIDVEKLIGKKLEFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAL8</accession>
    <name>F6BAL8_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Alpha-ribazole phosphatase CobZ</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1497</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97031.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799625.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1497"/>
    <dbReference type="EnsemblBacteria" id="AEF97031">
        <property type="protein sequence ID" value="AEF97031"/>
        <property type="gene ID" value="Metig_1497"/>
    </dbReference>
    <dbReference type="GeneID" id="10644370"/>
    <dbReference type="KEGG" id="mig:Metig_1497"/>
    <dbReference type="HOGENOM" id="CLU_120791_0_0_2"/>
    <dbReference type="OMA" id="YDAKKPG"/>
    <dbReference type="OrthoDB" id="73343at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008962">
        <property type="term" value="F:phosphatidylglycerophosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06971">
        <property type="entry name" value="PgpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036681">
        <property type="entry name" value="PgpA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017577">
        <property type="entry name" value="Ribazole_CobZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007686">
        <property type="entry name" value="YutG/PgpA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04608">
        <property type="entry name" value="PgpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101307">
        <property type="entry name" value="SSF101307"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03161">
        <property type="entry name" value="ribazole_CobZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PgpA" evidence="1">
        <location>
            <begin position="11"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04608"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF97031.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="141" mass="15861" checksum="307CD18BCCB79EB7" modified="2011-07-27" version="1">MNILKILENFGITLEDLVDSGMELCICEEEEKEKIRKKLKETIIKELNNPNVSTLLIAAVKLEEEGKKGNLPFNYDEDPNYIYVDEVIGMAIANEIAGTRAIFNFRWYDAKKPGIIKELGLFLDDAIAGLIAGCMSKVFSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAM7</accession>
    <name>F6BAM7_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcriptional regulator TrmB</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0285</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95841.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0285"/>
    <dbReference type="EnsemblBacteria" id="AEF95841">
        <property type="protein sequence ID" value="AEF95841"/>
        <property type="gene ID" value="Metig_0285"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0285"/>
    <dbReference type="HOGENOM" id="CLU_082928_1_0_2"/>
    <dbReference type="OMA" id="RAKIYIY"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002831">
        <property type="entry name" value="Tscrpt_reg_TrmB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01978">
        <property type="entry name" value="TrmB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TrmB" evidence="1">
        <location>
            <begin position="45"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF95841.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="159" mass="18224" checksum="176C380731211BA0" modified="2011-07-27" version="1">MSNETPDKIDKNDSMNNQKTEQIKKTIENVDKALINSLASLLSSEVKAKIYIYLRKYGESTVDEIAHGTGIYPSTVREIILEMYEEGLVNRKKLEKEGLGKRPYLYSAIPPSEVVKKLSKSLQKKLNDVFMLDKKLKKKEIKIPFLPVKIIIDDSNKEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAN5</accession>
    <name>F6BAN5_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Formylmethanofuran dehydrogenase subunit E region</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Metig_0293</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95849.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798458.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0293"/>
    <dbReference type="EnsemblBacteria" id="AEF95849">
        <property type="protein sequence ID" value="AEF95849"/>
        <property type="gene ID" value="Metig_0293"/>
    </dbReference>
    <dbReference type="GeneID" id="10643128"/>
    <dbReference type="KEGG" id="mig:Metig_0293"/>
    <dbReference type="HOGENOM" id="CLU_087508_0_0_2"/>
    <dbReference type="OMA" id="IGFRACE"/>
    <dbReference type="OrthoDB" id="90623at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026328">
        <property type="entry name" value="FmdE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003814">
        <property type="entry name" value="FmdEsu_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000962">
        <property type="entry name" value="Znf_DskA_TraR"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02663">
        <property type="entry name" value="FmdE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01258">
        <property type="entry name" value="zf-dskA_traR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006578">
        <property type="entry name" value="FwdE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="FmdE" evidence="5">
        <location>
            <begin position="11"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="domain" description="DksA C4-type" evidence="4">
        <location>
            <begin position="158"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEF95849.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="193" mass="22734" checksum="0A255C848D721A4C" modified="2011-07-27" version="1">MDEELKKVIEFHGHFCPGLAIGYRVAKYVKNHFKKSEDEELVAIVENNACGVDAIQYMLSCTFGKGNLIFKDNGKHVYTFYSRESNRAIRIYVKKNFFEEFDDILRKFNTGKLSEEEMQIFNQRRKEISEKILSMDEKELFDVREVDIEPPRKARLFPSIKCQECGEYFMEIKGRVINGKIVCKECFEKLLKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAN9</accession>
    <name>F6BAN9_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_0297</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95853.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798462.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0297"/>
    <dbReference type="EnsemblBacteria" id="AEF95853">
        <property type="protein sequence ID" value="AEF95853"/>
        <property type="gene ID" value="Metig_0297"/>
    </dbReference>
    <dbReference type="GeneID" id="10643132"/>
    <dbReference type="KEGG" id="mig:Metig_0297"/>
    <dbReference type="HOGENOM" id="CLU_1100981_0_0_2"/>
    <dbReference type="OMA" id="SYDFDIC"/>
    <dbReference type="OrthoDB" id="59518at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF95853.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="226" mass="26488" checksum="43CA714DA5DF4CCE" modified="2011-07-27" version="1">MLGIATKLSQINQNALIELNNKVYKCFDYHVIDAEKTPLTNENILENLRNVVLTVQSRRKDVFELLDFYTSYDFDICIVLGNRHYLSDEEKKYKRFGILEVINKCLKIKDNLWIGTEGVEDIVQQIIEDNDLIAFHLYGCECNINAKKAIYVPFASEINKDVLKSMENYLKRRKNYNGNWQDYLISLKDIKKDFIKNIKSKNDIIVGYPIKPHVHEIMHFKEVFGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-09-29" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAP1</accession>
    <name>F6BAP1_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ATPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0299</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95855.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF95855">
        <property type="protein sequence ID" value="AEF95855"/>
        <property type="gene ID" value="Metig_0299"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0299"/>
    <dbReference type="HOGENOM" id="CLU_068608_0_0_2"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011579">
        <property type="entry name" value="ATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01637">
        <property type="entry name" value="ATPase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATPase_2" evidence="1">
        <location>
            <begin position="7"/>
            <end position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF95855.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="359" mass="42563" checksum="9243B6940A485F0E" modified="2011-07-27" version="1">MVIKMKFFNREKEINEILHILNKEPDDIYFIYGPLNSGKTILINHIITKKLDKKYIPFYINFRRRAIINVDNFIECLFEVDEKSKIDDFREYAKSLADLLVKGSKELSNYYLGMPKNFFEKKEKSGDIYQYIEYLFAELNKKGKIPILIFDELQMIKELTLNGKRPLLKSLFQFLVSLTKEQHLCHVFCLTSDSLFAEYVYNTGELGGRAKYLLVDDFDKETALKFMDFIAKENNIKLSDEEKELVYSYVGGKVKDIKYVVEESKFKNLKEILEIILKEEKQKLKYFLEDVKEKDDELYKNIIDALKIFKEKYEVEDISIPKKVREFLIKKNILFLNPIEGTLKPQSFLIWNAIKKVIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAT1</accession>
    <name>F6BAT1_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Phosphoserine phosphatase</fullName>
            <ecNumber evidence="4">3.1.3.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">O-phosphoserine phosphohydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">Metig_0339</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-serine biosynthesis; L-serine from 3-phospho-D-glycerate: step 3/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the HAD-like hydrolase superfamily. SerB family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.3" evidence="4"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95895.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798504.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0339"/>
    <dbReference type="EnsemblBacteria" id="AEF95895">
        <property type="protein sequence ID" value="AEF95895"/>
        <property type="gene ID" value="Metig_0339"/>
    </dbReference>
    <dbReference type="GeneID" id="10643176"/>
    <dbReference type="KEGG" id="mig:Metig_0339"/>
    <dbReference type="HOGENOM" id="CLU_036368_4_3_2"/>
    <dbReference type="OMA" id="CINEPDL"/>
    <dbReference type="OrthoDB" id="105892at2157"/>
    <dbReference type="UniPathway" id="UPA00135">
        <property type="reaction ID" value="UER00198"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036424">
        <property type="term" value="F:L-phosphoserine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006564">
        <property type="term" value="P:L-serine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004469">
        <property type="entry name" value="PSP"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00029">
        <property type="entry name" value="phosphoserine_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="SSF56784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00338">
        <property type="entry name" value="serB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="7 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0718">Serine biosynthesis</keyword>
    <feature type="active site" description="Nucleophile" evidence="11">
        <location>
            <position position="12"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="11">
        <location>
            <position position="14"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00031693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR604469-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AEF95895.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="212" mass="23515" checksum="DF40FA2B3B0044F1" modified="2011-07-27" version="1">MEGKKKKIILFDFDSTLVDCETIDEIAKEAGVEEEVKKITKEAMEGKLNFEQSLRKRVSLLKGLPVEKVEKAVSNIKLMNGAEETIKELKKRGYVIGVVSGGFDIAVNRIKEKLGLDYAYANELIEKDGILTGEVRGPVMSETAKGDILEEIARKEGVDLKDTVVVGDGANDISMFNKAGLKIAFCAKEILKEKADICIEKKDLREILKHID</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-06-02" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAU0</accession>
    <name>F6BAU0_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_0348</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95904.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798513.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF95904">
        <property type="protein sequence ID" value="AEF95904"/>
        <property type="gene ID" value="Metig_0348"/>
    </dbReference>
    <dbReference type="GeneID" id="10643185"/>
    <dbReference type="KEGG" id="mig:Metig_0348"/>
    <dbReference type="HOGENOM" id="CLU_1159061_0_0_2"/>
    <dbReference type="OrthoDB" id="132939at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF95904.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="239" mass="27703" checksum="0FD8B9A762908733" modified="2011-07-27" version="1">MRLEDLLNIEVPKIDLSEEQVLARVLGYIVLNTKYGRTYKGIYLSEQGLGHFFTIKKPEIGDFLIKGAFIGKNNILELSRESVFERTEIDEKTINEWKKVALQQPFITGIKEEFFYNEIENRKESRYVALRVLKIIETERNVCESCGILLPSDKDFCECGGRAITVPTLKCITIDKFEHLGYWTIVDYRYEIPSLKYPTLILGHVLPKFINETRSHGGKEYKYTHDILAPGLFVELDKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAW0</accession>
    <name>F6BAW0_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF58 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_1513</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97047.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799641.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1513"/>
    <dbReference type="EnsemblBacteria" id="AEF97047">
        <property type="protein sequence ID" value="AEF97047"/>
        <property type="gene ID" value="Metig_1513"/>
    </dbReference>
    <dbReference type="GeneID" id="10644386"/>
    <dbReference type="KEGG" id="mig:Metig_1513"/>
    <dbReference type="HOGENOM" id="CLU_520352_0_0_2"/>
    <dbReference type="OMA" id="VYPSNES"/>
    <dbReference type="OrthoDB" id="48221at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002881">
        <property type="entry name" value="DUF58"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01882">
        <property type="entry name" value="DUF58"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="SSF53300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="domain" description="DUF58" evidence="2">
        <location>
            <begin position="176"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF97047.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="429" mass="50615" checksum="34A54F280333FAD7" modified="2011-07-27" version="1">MKVKNRDLLILVSLYFYGSGYVIGNITPVLIGGIILIYLYLSDSIFKKRIEEFYSKEVKVESNELKEHETSKIFIKFYSKGLILKFNFFSNEGKVEILEEREEKDYKEYILEITPQKKGDFILKVSGEIFDRVELNKVFYKNSFKFKVLPSVKSLVSELEKGESLSSTLRFYVEPEINELKRYEIGDDIRRIDWKKSLMSNELIVRKVLYKEKSPIYYLLDAGYSMRKCVNKKRSKIGYALDILISFLKSKNIENDEYIFIYDEFKVRDIIFVRKTKVEDIINRLIIEPIKIERYIPSEITIEGGSRDLIIYKYLSKGKKFGILNCVQHLLKMIPGVVIIITDLDSNISPLIKGVRLLNRKGFRVIIFSLYSPSFNIDKIDEELLKKLYEHYISRKKIIENLRRCGALVIDISYSDKINKIIKRMRRRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAW1</accession>
    <name>F6BAW1_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ATPase associated with various cellular activities AAA_3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1514</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97048.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799642.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1514"/>
    <dbReference type="EnsemblBacteria" id="AEF97048">
        <property type="protein sequence ID" value="AEF97048"/>
        <property type="gene ID" value="Metig_1514"/>
    </dbReference>
    <dbReference type="GeneID" id="10644387"/>
    <dbReference type="KEGG" id="mig:Metig_1514"/>
    <dbReference type="HOGENOM" id="CLU_034716_2_0_2"/>
    <dbReference type="OMA" id="SRAWAWL"/>
    <dbReference type="OrthoDB" id="49734at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011703">
        <property type="entry name" value="ATPase_AAA-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041628">
        <property type="entry name" value="ChlI/MoxR_AAA_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07726">
        <property type="entry name" value="AAA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17863">
        <property type="entry name" value="AAA_lid_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA" evidence="1">
        <location>
            <begin position="31"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF97048.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="309" mass="35084" checksum="26A8928AB06DE8D5" modified="2011-07-27" version="1">MNAFEMIKKEIKKNIIGLDDIIRDLFVCLVSEGHILLEGAPGLAKTTLAKSFAESLNLKFSRIQGCPDLLPSDLTGGEIYRLQSGTFEFIEGPIFANIVLVDEINRMSPKTQSGLLEAMEERTVTIGRKTYSLPRPFIVIATQNPVEFEGTYPLSTAQIDRFMMKINMNYLSEEEEFEVLKMKLHNGGDEIKPVVDKEMLNNIIKEITNIHVSLPILKYIKDIIVKTRKDERLILGASTRAGIHLLKVAKVYAYLDNRTYVIPDDVKNNVVKVLSHRLIVDYEEESSAEEILKDILKDVEVPKGDFRFH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAW9</accession>
    <name>F6BAW9_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Glycosyl transferase group 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_1522</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97056.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799650.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF97056">
        <property type="protein sequence ID" value="AEF97056"/>
        <property type="gene ID" value="Metig_1522"/>
    </dbReference>
    <dbReference type="GeneID" id="10644395"/>
    <dbReference type="KEGG" id="mig:Metig_1522"/>
    <dbReference type="HOGENOM" id="CLU_009583_2_5_2"/>
    <dbReference type="OMA" id="VSHKYPP"/>
    <dbReference type="OrthoDB" id="66448at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028098">
        <property type="entry name" value="Glyco_trans_4-like_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13439">
        <property type="entry name" value="Glyco_transf_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glyco_trans_4-like_N" evidence="2">
        <location>
            <begin position="43"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="domain" description="Glycos_transf_1" evidence="1">
        <location>
            <begin position="158"/>
            <end position="330"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF97056.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="357" mass="40758" checksum="D80F323EA8E616AD" modified="2011-07-27" version="1">MNILFIARNYPPQVGGAERLNYELIQNLKNLMDIEVIPNKFGKYSPLFHIYAVFKAVLNRKNIEVVFLSDAYLSPIIPIIKLILKKPVVIKVHGLDITFSNKIYQLIIPRFVNMADKVICISNATKKECIRRGVSPDKCKVILIGINGKDPNDHMDEKAYSKFNIDEHKYILLSVGRLVERKGIHWFVENVIPKLIKKEKNITYVIAGDGPYRETIENIILSKGLEDYVKLLGKVSDDDLELLYNIADIFIMPNIPVDGDMEGFGIVVLEASSHGVPVIASDLEGIRDAVINGKTGFLVEPLNEDAYVEKILEMLDKVKKDRKMKENIKKLTLQNYNWDKISQEYYDVFCSVIKNKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAZ2</accession>
    <name>F6BAZ2_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Probable transcription termination protein NusA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">nusA</name>
        <name evidence="9" type="ordered locus">Metig_1545</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Participates in transcription termination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the NusA family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97079.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799673.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1545"/>
    <dbReference type="EnsemblBacteria" id="AEF97079">
        <property type="protein sequence ID" value="AEF97079"/>
        <property type="gene ID" value="Metig_1545"/>
    </dbReference>
    <dbReference type="GeneID" id="10644418"/>
    <dbReference type="KEGG" id="mig:Metig_1545"/>
    <dbReference type="HOGENOM" id="CLU_131906_1_0_2"/>
    <dbReference type="OMA" id="TDQIKYI"/>
    <dbReference type="OrthoDB" id="99842at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006353">
        <property type="term" value="P:DNA-templated transcription, termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031564">
        <property type="term" value="P:transcription antitermination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00945_A">
        <property type="entry name" value="NusA_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015946">
        <property type="entry name" value="KH_dom-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025249">
        <property type="entry name" value="KH_dom_NusA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009019">
        <property type="entry name" value="KH_sf_prok-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010212">
        <property type="entry name" value="NusA_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030842">
        <property type="entry name" value="NusA_bac"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22648">
        <property type="entry name" value="PTHR22648"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13184">
        <property type="entry name" value="KH_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54814">
        <property type="entry name" value="SSF54814"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01952">
        <property type="entry name" value="nusA_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5 6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="1 6" id="KW-0806">Transcription termination</keyword>
    <feature type="domain" description="KH" evidence="8">
        <location>
            <begin position="30"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="159"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="164"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AEF97079.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="196" mass="22457" checksum="F909C7CEDFBFCE90" modified="2011-07-27" version="1">MKVRLTTEEIMKIGYFEKLSGATVIDCICDDDRIVFVVKEGDIGAAIGKGGENVKTAMEKFGKKIDIIEYSSDLKKFIKNIFAPIELEDVWFKKINGELIAYVRINPKFRRAVIGNRGRNIERAVQIARRHTDVKNIRVIVGNRRRPFRKRFGGKKVLRDTSKESVEMQAQATKTEETNTEQQQTETTNTTTEENQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-06-02" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>F6BB48</accession>
    <name>F6BB48_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_0377</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95933.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798542.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF95933">
        <property type="protein sequence ID" value="AEF95933"/>
        <property type="gene ID" value="Metig_0377"/>
    </dbReference>
    <dbReference type="GeneID" id="10643214"/>
    <dbReference type="KEGG" id="mig:Metig_0377"/>
    <dbReference type="HOGENOM" id="CLU_083800_0_0_2"/>
    <dbReference type="OMA" id="RGVFSWT"/>
    <dbReference type="OrthoDB" id="52283at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF95933.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="225" mass="25890" checksum="EA9C82C0F29EC366" modified="2011-07-27" version="1">MKPKYALRKDMVAEFTLNKSFNTYRGRVIKADFNGPLEGVVMVNKKEHVYFYPLRALHMIRPLNCIPTNVVPKTSLPTNPKNVHVKEALSRIVGRTLKVCYKNPKTSYLGRLLGFTRGVFSWTLALEIHGETVLLINPSYISYYGTKWILPKNNAPFKPPKLMNLTKTTNYLKRCLLDEVKLEPNYPRINIEDKVYLYPYGIVSNDKILADHVATLLKEQGFIID</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>F6BB63</accession>
    <name>F6BB63_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">30S ribosomal protein S15</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">rps15</name>
        <name evidence="7" type="ordered locus">Metig_0392</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="3">Part of the 30S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 4">Belongs to the universal ribosomal protein uS15 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95948.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798557.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0392"/>
    <dbReference type="EnsemblBacteria" id="AEF95948">
        <property type="protein sequence ID" value="AEF95948"/>
        <property type="gene ID" value="Metig_0392"/>
    </dbReference>
    <dbReference type="GeneID" id="10643229"/>
    <dbReference type="KEGG" id="mig:Metig_0392"/>
    <dbReference type="HOGENOM" id="CLU_090139_2_0_2"/>
    <dbReference type="OMA" id="EEHPKDM"/>
    <dbReference type="OrthoDB" id="98179at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00353">
        <property type="entry name" value="Ribosomal_S15p_S13e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01343_A">
        <property type="entry name" value="Ribosomal_S15_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012606">
        <property type="entry name" value="Ribosomal_S13/S15_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000589">
        <property type="entry name" value="Ribosomal_S15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023029">
        <property type="entry name" value="Ribosomal_S15P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009068">
        <property type="entry name" value="S15_NS1_RNA-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11885">
        <property type="entry name" value="PTHR11885"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08069">
        <property type="entry name" value="Ribosomal_S13_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00312">
        <property type="entry name" value="Ribosomal_S15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01386">
        <property type="entry name" value="Ribosomal_S13_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01387">
        <property type="entry name" value="Ribosomal_S15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47060">
        <property type="entry name" value="SSF47060"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00362">
        <property type="entry name" value="RIBOSOMAL_S15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="1 3 4" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Ribosomal_S13_N" evidence="6">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM01386"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEF95948.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="151" mass="17678" checksum="F874C7ED60184444" modified="2011-07-27" version="1">MARLHSDRRGSSGSKRPLRKEVPEWVTLKPEEVEELVVKLAKEGYQSAMIGMILRDTYGIPDVKLITGKKISQIMKEHGVYPEVPEDLLNLMRRAVNLRNHLEQHPKDLHSKRGLQLIESKIRRLVKYYKRKGVLPMDWRYTPEKARLLVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBB9</accession>
    <name>F6BBB9_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Molybdenum cofactor synthesis domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metig_1593</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the MoaB/Mog family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97126.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799719.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1593"/>
    <dbReference type="EnsemblBacteria" id="AEF97126">
        <property type="protein sequence ID" value="AEF97126"/>
        <property type="gene ID" value="Metig_1593"/>
    </dbReference>
    <dbReference type="GeneID" id="10644466"/>
    <dbReference type="KEGG" id="mig:Metig_1593"/>
    <dbReference type="HOGENOM" id="CLU_077358_2_3_2"/>
    <dbReference type="OMA" id="TGWDGIL"/>
    <dbReference type="OrthoDB" id="108340at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006777">
        <property type="term" value="P:Mo-molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00886">
        <property type="entry name" value="MogA_MoaB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.980.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012245">
        <property type="entry name" value="MoaB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036425">
        <property type="entry name" value="MoaB/Mog-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001453">
        <property type="entry name" value="MoaB/Mog_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008284">
        <property type="entry name" value="MoCF_biosynth_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43232">
        <property type="entry name" value="PTHR43232"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00994">
        <property type="entry name" value="MoCF_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006443">
        <property type="entry name" value="MoaB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00852">
        <property type="entry name" value="MoCF_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53218">
        <property type="entry name" value="SSF53218"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00177">
        <property type="entry name" value="molyb_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01078">
        <property type="entry name" value="MOCF_BIOSYNTHESIS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0501">Molybdenum cofactor biosynthesis</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MoCF_biosynth" evidence="3">
        <location>
            <begin position="11"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEF97126.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="163" mass="18162" checksum="CE66736AA888C8D8" modified="2011-07-27" version="1">MHEGISDIKYGVITVSDSRYNEMLKGNNKVEDKSGELLKNELNAIFHALIPDNKDMIKGVIDHVIDFFDVDCIVITGGTGISKRDTTPDVLKEIFEKELEGFKIIFHKISYEEIGTATILSRATAGIYKGRVIYALPGSVNACKTALKIIKKETGHILKHVRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBJ3</accession>
    <name>F6BBJ3_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ribose 1,5-bisphosphate isomerase</fullName>
            <shortName evidence="3">R15P isomerase</shortName>
            <shortName evidence="3">R15Pi</shortName>
            <ecNumber evidence="3">5.3.1.29</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Ribulose 1,5-bisphosphate synthase</fullName>
            <shortName evidence="3">RuBP synthase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metig_0446</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the isomerization of ribose 1,5-bisphosphate (R15P) to ribulose 1,5-bisphosphate (RuBP), the CO(2) acceptor and substrate for RubisCO. Functions in an archaeal AMP degradation pathway, together with AMP phosphorylase and RubisCO.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>alpha-D-ribose 1,5-bisphosphate = D-ribulose 1,5-bisphosphate</text>
            <dbReference type="Rhea" id="RHEA:32243"/>
            <dbReference type="ChEBI" id="CHEBI:57870"/>
            <dbReference type="ChEBI" id="CHEBI:68688"/>
            <dbReference type="EC" id="5.3.1.29"/>
        </reaction>
    </comment>
    <comment type="miscellaneous">
        <text evidence="3">Reaction proceeds via a cis-phosphoenolate intermediate.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the eIF-2B alpha/beta/delta subunits family. R15P isomerase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.29" evidence="3"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96002.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798611.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0446"/>
    <dbReference type="EnsemblBacteria" id="AEF96002">
        <property type="protein sequence ID" value="AEF96002"/>
        <property type="gene ID" value="Metig_0446"/>
    </dbReference>
    <dbReference type="GeneID" id="10643283"/>
    <dbReference type="KEGG" id="mig:Metig_0446"/>
    <dbReference type="HOGENOM" id="CLU_016218_2_1_2"/>
    <dbReference type="OMA" id="GDVIMTH"/>
    <dbReference type="OrthoDB" id="36100at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043917">
        <property type="term" value="F:ribose 1,5-bisphosphate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019323">
        <property type="term" value="P:pentose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10470">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02230">
        <property type="entry name" value="R15P_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000649">
        <property type="entry name" value="IF-2B-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042529">
        <property type="entry name" value="IF_2B-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011559">
        <property type="entry name" value="Initiation_fac_2B_a/b/d"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027363">
        <property type="entry name" value="M1Pi_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005250">
        <property type="entry name" value="Ribulose_e2b2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01008">
        <property type="entry name" value="IF-2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="SSF100950"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00524">
        <property type="entry name" value="eIF-2B_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00511">
        <property type="entry name" value="ribulose_e2b2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="4" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="2 3 4" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Substrate binding" evidence="3">
        <location>
            <begin position="17"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="3">
        <location>
            <begin position="201"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="3">
        <location>
            <position position="122"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="3">
        <location>
            <position position="191"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="60"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="227"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEF96002.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="303" mass="33940" checksum="37DD0570089519CA" modified="2011-07-27" version="1">MDIIKETYEKIKNMEVRGAGRIARAAANALKEYAETIMHLDDEEFKKKILEAGELLRSARPTAVSLPNAVKYVLNGLKDENPKESVIKKAEEFIISSKNATKNIGKIGAKRIRDGYTILTHCNSEAAIEVIKTAYHEGKDIKVFCTETRPRNQGYLTAKALCDEGIDVTLIVDSAVRYFIKEVDIVVVGADAITANGCLVNKIGTSQIALIAHEARIPFLTAAETYKFHPKTIVGELIEIEERNPEEVVEFEGKYKKIKIRNPAFDVTPSQYIDAIITEIGLIPPQGAWYIIEKYFGWIEHEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBK1</accession>
    <name>F6BBK1_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Precorrin-3B C17-methyltransferase</fullName>
            <ecNumber evidence="6">2.1.1.131</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Metig_0454</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; adenosylcobalamin biosynthesis.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.131" evidence="6"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96010.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798619.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0454"/>
    <dbReference type="EnsemblBacteria" id="AEF96010">
        <property type="protein sequence ID" value="AEF96010"/>
        <property type="gene ID" value="Metig_0454"/>
    </dbReference>
    <dbReference type="GeneID" id="10643291"/>
    <dbReference type="KEGG" id="mig:Metig_0454"/>
    <dbReference type="HOGENOM" id="CLU_047948_2_0_2"/>
    <dbReference type="OMA" id="VWDDRMV"/>
    <dbReference type="OrthoDB" id="49464at2157"/>
    <dbReference type="UniPathway" id="UPA00148"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030789">
        <property type="term" value="F:precorrin-3B C17-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd11646">
        <property type="entry name" value="Precorrin_3B_C17_MT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.950.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1010.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000878">
        <property type="entry name" value="4pyrrol_Mease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035996">
        <property type="entry name" value="4pyrrol_Methylase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014777">
        <property type="entry name" value="4pyrrole_Mease_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014776">
        <property type="entry name" value="4pyrrole_Mease_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006363">
        <property type="entry name" value="Cbl_synth_CobJ/CibH_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00590">
        <property type="entry name" value="TP_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53790">
        <property type="entry name" value="SSF53790"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01466">
        <property type="entry name" value="cobJ_cbiH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 6" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="TP_methylase" evidence="5">
        <location>
            <begin position="1"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEF96010.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="252" mass="28504" checksum="1BF82A415F609E82" modified="2011-07-27" version="1">MLYVVGIGPGGEEHLTKKAEEILKNVDLIVCYKGYKKFVERFNKPIYTTGMKKEIERVEYALRESKDKDVALVSSGDATIYGLASLAYELNEKKNYNVEIEVIPGITSASSCSAILGAPLNHDFVVLSLSDLLTPLDIILKRVRCALEGDFVIAIYNPMSKSRKEPFLKTIEIVKRYAEKNNTNYIIGIVKNAGRKNEEYKIITINELINNLEKYMEFIDMNTTLIIGNSNTKIIDGKMVTPRGYLNKYKIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBM9</accession>
    <name>F6BBM9_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_0482</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96038.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798646.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0482"/>
    <dbReference type="EnsemblBacteria" id="AEF96038">
        <property type="protein sequence ID" value="AEF96038"/>
        <property type="gene ID" value="Metig_0482"/>
    </dbReference>
    <dbReference type="GeneID" id="10643319"/>
    <dbReference type="KEGG" id="mig:Metig_0482"/>
    <dbReference type="HOGENOM" id="CLU_145179_0_0_2"/>
    <dbReference type="OMA" id="KYTIIEI"/>
    <dbReference type="OrthoDB" id="103140at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011322">
        <property type="entry name" value="N-reg_PII-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019296">
        <property type="entry name" value="Unchr_N-regulatory-PII-rel"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10126">
        <property type="entry name" value="Nit_Regul_Hom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54913">
        <property type="entry name" value="SSF54913"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF96038.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="111" mass="12533" checksum="9E4879477C94EEFF" modified="2011-07-27" version="1">MKVLVYLFIRSEDLGKAINALTEGGITGFFLYDYKGMSPQDWKGFLLNEDPESAVKIINDLSENAILIGTVLNTESVETLEKVIHDRLANDKYTIIEIPIEDIKVNMPDKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBS3</accession>
    <name>F6BBS3_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Serine/threonine protein kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Metig_1671</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97203.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1671"/>
    <dbReference type="EnsemblBacteria" id="AEF97203">
        <property type="protein sequence ID" value="AEF97203"/>
        <property type="gene ID" value="Metig_1671"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_1671"/>
    <dbReference type="HOGENOM" id="CLU_283650_0_0_2"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006468">
        <property type="term" value="P:protein phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018765">
        <property type="entry name" value="DUF2341"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10102">
        <property type="entry name" value="DUF2341"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="SSF56112"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="5">
        <location>
            <begin position="721"/>
            <end position="981"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="624"/>
            <end position="664"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="689"/>
            <end position="710"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="973"/>
            <end position="1010"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="696"/>
            <end position="710"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEF97203.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="1097" mass="123664" checksum="A474AC762B31CB0E" modified="2011-07-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBV6</accession>
    <name>F6BBV6_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">PDGLE domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Metig_1704</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97236.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799826.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1704"/>
    <dbReference type="EnsemblBacteria" id="AEF97236">
        <property type="protein sequence ID" value="AEF97236"/>
        <property type="gene ID" value="Metig_1704"/>
    </dbReference>
    <dbReference type="GeneID" id="10644577"/>
    <dbReference type="KEGG" id="mig:Metig_1704"/>
    <dbReference type="HOGENOM" id="CLU_137910_1_0_2"/>
    <dbReference type="OMA" id="SPMPDYA"/>
    <dbReference type="OrthoDB" id="126698at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025937">
        <property type="entry name" value="PDGLE_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13190">
        <property type="entry name" value="PDGLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="7"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="79"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="domain" description="PDGLE" evidence="6">
        <location>
            <begin position="13"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF13190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEF97236.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="104" mass="11081" checksum="F0C2FD34F7B70C07" modified="2011-07-27" version="1">MDNKKILLYGLVISLIIGVLAPFLACPNPDGLESTAEKIINEKTLHHNLQQIGLEEEGTVVPSPMPDYSIEGMGKVGEVIAMIVGIIIILAVAYGITVAIAKKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBW1</accession>
    <name>F6BBW1_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Magnesium transport protein CorA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">corA</name>
        <name evidence="8" type="ordered locus">Metig_1709</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Mediates influx of magnesium ions.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Membrane</location>
            <topology evidence="1 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the CorA metal ion transporter (MIT) (TC 1.A.35) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97241.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799830.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1709"/>
    <dbReference type="EnsemblBacteria" id="AEF97241">
        <property type="protein sequence ID" value="AEF97241"/>
        <property type="gene ID" value="Metig_1709"/>
    </dbReference>
    <dbReference type="GeneID" id="10644582"/>
    <dbReference type="KEGG" id="mig:Metig_1709"/>
    <dbReference type="HOGENOM" id="CLU_007127_0_0_2"/>
    <dbReference type="OMA" id="GILHTDH"/>
    <dbReference type="OrthoDB" id="43588at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015087">
        <property type="term" value="F:cobalt ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015095">
        <property type="term" value="F:magnesium ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045861">
        <property type="entry name" value="CorA_cytoplasmic_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045863">
        <property type="entry name" value="CorA_TM1_TM2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004488">
        <property type="entry name" value="Mg/Co-transport_prot_CorA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002523">
        <property type="entry name" value="MgTranspt_CorA/ZnTranspt_ZntB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01544">
        <property type="entry name" value="CorA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143865">
        <property type="entry name" value="SSF143865"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144083">
        <property type="entry name" value="SSF144083"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00383">
        <property type="entry name" value="corA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="256"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="290"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="141"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU362010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEF97241.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="317" mass="37343" checksum="965DBD95EAD65682" modified="2011-07-27" version="1">MLRVIGYDGVVKEITLDDINENYTLIWIDCYDPSNEELAKLSKKIEIDVNELSIGLDEQEVPRVEEEDDYYLIIYKAPLFEEDITTTSFGIFIKDNILLTIHSDKIKAIGRLYNLLKTKSPKILFERGIGFFLYNLLNQITRSYSRILLNLEDELDVLEDRLLVEYNKQITEEILQLRKMLVYFHKALISNKEVLSVLKRKYLPITTQEDRANFEDLYYDTLQLIDMETTYRELLVSMMDMCLSLENIRMNQVMKILTMVTTLFAVPMWITGIFGMNFKYMPLVDSPNGFWVVMGISLLVLFALAYVFGREKWFKLW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBX5</accession>
    <name>F6BBX5_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Methanogenesis marker 13 metalloprotein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1723</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97255.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799844.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1723"/>
    <dbReference type="EnsemblBacteria" id="AEF97255">
        <property type="protein sequence ID" value="AEF97255"/>
        <property type="gene ID" value="Metig_1723"/>
    </dbReference>
    <dbReference type="GeneID" id="10644597"/>
    <dbReference type="KEGG" id="mig:Metig_1723"/>
    <dbReference type="HOGENOM" id="CLU_782144_0_0_2"/>
    <dbReference type="OMA" id="CASMIIG"/>
    <dbReference type="OrthoDB" id="56870at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017675">
        <property type="entry name" value="CfbD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000510">
        <property type="entry name" value="Nase/OxRdtase_comp1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00148">
        <property type="entry name" value="Oxidored_nitro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03282">
        <property type="entry name" value="methan_mark_13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Oxidored_nitro" evidence="1">
        <location>
            <begin position="23"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF97255.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="355" mass="40093" checksum="8750F721A977FCDF" modified="2011-07-27" version="1">MILHPRPSPIAAAMYQLRDVGVDAIILHGPAGCCFRTARLLELDGVRVFTSAMDENDFIFGAMDKLRRTIEEVIEYLKEHKNDGNYMIGIVGTCASMIIGEDIWSVVDEYDATLIPVEVHSGLNDNTIGAINAMESCLKLGLIDEKEFERQKYMLKKATEIEKKRGMAKSKYIKPTYEDDLKDVIELFKNNMNKNPKVACVLNAKKETAYLFAHPLIKINEIFNNCINIGNLDENIGLKKIREDAKNILREFKVDYITGGLDEYPVTGEKAVEILKEIKPDIVVVSGVPHALPIEELKEEVDCITIGVSDGPRLYYPIKEYYDYAVIELDAHAKVLGKREVVKSRFGEILEYALK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBY0</accession>
    <name>F6BBY0_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Alpha-amylase</fullName>
            <ecNumber evidence="2">3.2.1.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1728</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.2.1.1" evidence="2"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97260.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799849.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1728"/>
    <dbReference type="EnsemblBacteria" id="AEF97260">
        <property type="protein sequence ID" value="AEF97260"/>
        <property type="gene ID" value="Metig_1728"/>
    </dbReference>
    <dbReference type="GeneID" id="10644602"/>
    <dbReference type="KEGG" id="mig:Metig_1728"/>
    <dbReference type="HOGENOM" id="CLU_033691_0_0_2"/>
    <dbReference type="OMA" id="GWRSPNY"/>
    <dbReference type="OrthoDB" id="45403at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004556">
        <property type="term" value="F:alpha-amylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011330">
        <property type="entry name" value="Glyco_hydro/deAcase_b/a-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004300">
        <property type="entry name" value="Glyco_hydro_57_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03065">
        <property type="entry name" value="Glyco_hydro_57"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88713">
        <property type="entry name" value="SSF88713"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glyco_hydro_57" evidence="1">
        <location>
            <begin position="5"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF97260.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="406" mass="48561" checksum="BEFB964B72E392FB" modified="2011-07-27" version="1">MLISFNFEVHQPRRLRKYIKKDSENLWERYVDDELNREIFNRVANKCYIPANRIILDLIDNYDFKISYSITGVFLEQAMAFNDDVLDLFKDLVKTGNVELICETYYHSLSSLFENHEEFVEEIKLHQKIIKEVFGYKPKVFRNTELIYHNKIAETIKKLGFEGIFTEGTEKVLEWRSPNYVYEALCGLKVLLRNYRLSDDIGFRFSCQNWEEYPLTADKYALWLSKTPGDCINIYMDYETFGEHQWRETGIFEFLKHLPQEIEKYEHLQFATPSEILKKCKTRGKIDVFEFSTISWADTERNVSAWLGNRMQKISFEKLKEIGKYIKEHKESIKNYEDVYKIYKILQTSDNFYYMCTKGLSDMDVHSYFSHFETPFDAYASYLNILYDFESYIVTNQKLNGYTFLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBY4</accession>
    <name>F6BBY4_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Starch synthase</fullName>
            <ecNumber evidence="5">2.4.1.21</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Metig_1732</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.4.1.21" evidence="5"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97264.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799853.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1732"/>
    <dbReference type="EnsemblBacteria" id="AEF97264">
        <property type="protein sequence ID" value="AEF97264"/>
        <property type="gene ID" value="Metig_1732"/>
    </dbReference>
    <dbReference type="GeneID" id="10644606"/>
    <dbReference type="KEGG" id="mig:Metig_1732"/>
    <dbReference type="HOGENOM" id="CLU_545885_0_0_2"/>
    <dbReference type="OMA" id="HNEAFKG"/>
    <dbReference type="OrthoDB" id="83551at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033201">
        <property type="term" value="F:alpha-1,4-glucan synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009011">
        <property type="term" value="F:starch synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013534">
        <property type="entry name" value="Starch_synth_cat_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08323">
        <property type="entry name" value="Glyco_transf_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 5" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glyco_transf_5" evidence="4">
        <location>
            <begin position="2"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="domain" description="Glycos_transf_1" evidence="3">
        <location>
            <begin position="299"/>
            <end position="447"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF08323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEF97264.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="522" mass="59136" checksum="B42ACA672F2A037D" modified="2011-07-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-06-02" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBY8</accession>
    <name>F6BBY8_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0513</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96069.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798677.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96069">
        <property type="protein sequence ID" value="AEF96069"/>
        <property type="gene ID" value="Metig_0513"/>
    </dbReference>
    <dbReference type="GeneID" id="10643350"/>
    <dbReference type="KEGG" id="mig:Metig_0513"/>
    <dbReference type="HOGENOM" id="CLU_2748193_0_0_2"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="5"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96069.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="70" mass="8345" checksum="854AC421702DA304" modified="2011-07-27" version="1">MGMGVKSILDEIRRLKRENAQLIKEKKELQKMRDELYAMMVEIEEQIQRNNARINKLIQEAKEVKRFGAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>F6BC15</accession>
    <name>F6BC15_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Cobaltochelatase., Magnesium chelatase</fullName>
            <ecNumber evidence="3">6.6.1.1</ecNumber>
            <ecNumber evidence="3">6.6.1.2</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_0542</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Mg-chelatase subunit H family.</text>
    </comment>
    <dbReference type="EC" id="6.6.1.1" evidence="3"/>
    <dbReference type="EC" id="6.6.1.2" evidence="3"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96096.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798703.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0542"/>
    <dbReference type="EnsemblBacteria" id="AEF96096">
        <property type="protein sequence ID" value="AEF96096"/>
        <property type="gene ID" value="Metig_0542"/>
    </dbReference>
    <dbReference type="GeneID" id="10643380"/>
    <dbReference type="KEGG" id="mig:Metig_0542"/>
    <dbReference type="HOGENOM" id="CLU_002017_1_0_2"/>
    <dbReference type="OMA" id="WETGQAM"/>
    <dbReference type="OrthoDB" id="344at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051116">
        <property type="term" value="F:cobaltochelatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016851">
        <property type="term" value="F:magnesium chelatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015995">
        <property type="term" value="P:chlorophyll biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd10150">
        <property type="entry name" value="CobN_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011771">
        <property type="entry name" value="BchH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011953">
        <property type="entry name" value="Cobalto_CobN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003672">
        <property type="entry name" value="CobN/Mg_chltase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44119">
        <property type="entry name" value="PTHR44119"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02514">
        <property type="entry name" value="CobN-Mg_chel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02025">
        <property type="entry name" value="BchH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02257">
        <property type="entry name" value="cobalto_cobN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="606"/>
            <end position="633"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010851"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF96096.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="1227" mass="141705" checksum="6E34357C165C26B5" modified="2011-07-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F6BC18</accession>
    <name>F6BC18_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">PKD domain containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0545</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96099.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0545"/>
    <dbReference type="EnsemblBacteria" id="AEF96099">
        <property type="protein sequence ID" value="AEF96099"/>
        <property type="gene ID" value="Metig_0545"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0545"/>
    <dbReference type="HOGENOM" id="CLU_398292_0_0_2"/>
    <dbReference type="OMA" id="ISGYIFI"/>
    <dbReference type="OrthoDB" id="16560at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008965">
        <property type="entry name" value="CBM2/CBM3_carb-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022409">
        <property type="entry name" value="PKD/Chitinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000601">
        <property type="entry name" value="PKD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035986">
        <property type="entry name" value="PKD_dom_sf"/>
    </dbReference>
    <dbReference type="SMART" id="SM00089">
        <property type="entry name" value="PKD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49299">
        <property type="entry name" value="SSF49299"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49384">
        <property type="entry name" value="SSF49384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50093">
        <property type="entry name" value="PKD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PKD" evidence="1">
        <location>
            <begin position="439"/>
            <end position="482"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96099.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="668" mass="76003" checksum="B400B831F89F8506" modified="2011-07-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>F6BC25</accession>
    <name>F6BC25_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="8">ABC-type transporter, integral membrane subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Metig_0552</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the binding-protein-dependent transport system permease family. FecCD subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96106.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798713.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0552"/>
    <dbReference type="EnsemblBacteria" id="AEF96106">
        <property type="protein sequence ID" value="AEF96106"/>
        <property type="gene ID" value="Metig_0552"/>
    </dbReference>
    <dbReference type="GeneID" id="10643390"/>
    <dbReference type="KEGG" id="mig:Metig_0552"/>
    <dbReference type="HOGENOM" id="CLU_013016_0_0_2"/>
    <dbReference type="OMA" id="LLIRNPM"/>
    <dbReference type="OrthoDB" id="36067at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3470.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037294">
        <property type="entry name" value="ABC_BtuC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000522">
        <property type="entry name" value="ABC_transptr_permease_BtuC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30472">
        <property type="entry name" value="PTHR30472"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01032">
        <property type="entry name" value="FecCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81345">
        <property type="entry name" value="SSF81345"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="52"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="95"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="122"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="147"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="196"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="239"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="281"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="312"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEF96106.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="337" mass="37057" checksum="F6083D3BC59AF4EF" modified="2011-07-27" version="1">MRKTLIILLLVILTSSLFVYGIFKGGNAKSISMEDVKNFLLYGSTGDKFKDILIWKIRIPPLITAIIVGAVLSVSGLKLQTLFRNLLASPYTTGISSGVVLGVAITIFLGFSFSSFLGVSDYVVGGWIGAAIALVIILFLASKIKDVSGVLVCTLLFGYFYYGIESYLINFADNVQIQEFWMYLQGSFSGTGWEDIKLMVVCSLIFLISSYLLSKHLNALLFGENYAKSFGLNIKQVRIIILFLSGFIVGVVIPFVGLIPFIGIASPYIARILMKTSDHRWTIPASMLIGMVISLMCYLVSIKLFAPRVVPVKSILDLLGGLLVVYLIYKSEKNMAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>F6BC34</accession>
    <name>F6BC34_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Na+/Ca+ antiporter, CaCA family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Metig_0561</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96115.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798722.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0561"/>
    <dbReference type="EnsemblBacteria" id="AEF96115">
        <property type="protein sequence ID" value="AEF96115"/>
        <property type="gene ID" value="Metig_0561"/>
    </dbReference>
    <dbReference type="GeneID" id="10643399"/>
    <dbReference type="KEGG" id="mig:Metig_0561"/>
    <dbReference type="HOGENOM" id="CLU_007948_0_1_2"/>
    <dbReference type="OMA" id="TRDYGVM"/>
    <dbReference type="OrthoDB" id="45269at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1420.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004481">
        <property type="entry name" value="K/Na/Ca-exchanger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004837">
        <property type="entry name" value="NaCa_Exmemb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044880">
        <property type="entry name" value="NCX_ion-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10846">
        <property type="entry name" value="PTHR10846"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01699">
        <property type="entry name" value="Na_Ca_ex"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00367">
        <property type="entry name" value="TIGR00367"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="38"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="74"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="108"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="158"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="196"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="225"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="254"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="279"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="domain" description="Na_Ca_ex" evidence="6">
        <location>
            <begin position="5"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="domain" description="Na_Ca_ex" evidence="6">
        <location>
            <begin position="162"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEF96115.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="298" mass="31973" checksum="3BA2B6ADFFC4DA53" modified="2011-07-27" version="1">MIINVLLFIIGLLLLSYGSDWFIVGSSRIAKLFRISDFVIGATIVAIGTSLPEIVTSVYAAWINSPGISTGNAIGSCIANVGLVLALSIIFSPIIIKESSVVKNTYAYILFLITAFILGFNGFSWIDGIILIFLFAIYLKYTIKNGKEKIDEVEETSYIRAALLLIIGLIGVILGSEMFVNGARNIALALGVSEKIVGFTLVALGTSLPELAVSIAAAKKKLGKMVLGNIVGSNVANIGMALGLSAIVTPLPPQNLEMGITLFMAVLLAMFAKRRKLGIFEGIVFLIMYCVFLYTLFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>F6BC42</accession>
    <name>F6BC42_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="1 6 7">Histidinol dehydrogenase</fullName>
            <shortName evidence="6 7">HDH</shortName>
            <ecNumber evidence="6 7">1.1.1.23</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">hisD</name>
        <name evidence="13" type="ordered locus">Metig_0569</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6 7">Catalyzes the sequential NAD-dependent oxidations of L-histidinol to L-histidinaldehyde and then to L-histidine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 7">
            <text>H2O + L-histidinol + 2 NAD(+) = 3 H(+) + L-histidine + 2 NADH</text>
            <dbReference type="Rhea" id="RHEA:20641"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57595"/>
            <dbReference type="ChEBI" id="CHEBI:57699"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.23"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6 11">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="6 11">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="6 7">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 9/9.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6 7 12">Belongs to the histidinol dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.23" evidence="6 7"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96123.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798730.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0569"/>
    <dbReference type="EnsemblBacteria" id="AEF96123">
        <property type="protein sequence ID" value="AEF96123"/>
        <property type="gene ID" value="Metig_0569"/>
    </dbReference>
    <dbReference type="GeneID" id="10643407"/>
    <dbReference type="KEGG" id="mig:Metig_0569"/>
    <dbReference type="HOGENOM" id="CLU_006732_3_2_2"/>
    <dbReference type="OMA" id="MVTGPGN"/>
    <dbReference type="OrthoDB" id="55330at2157"/>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00014"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004399">
        <property type="term" value="F:histidinol dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06572">
        <property type="entry name" value="Histidinol_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01024">
        <property type="entry name" value="HisD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001692">
        <property type="entry name" value="Histidinol_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022695">
        <property type="entry name" value="Histidinol_DH_monofunct"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012131">
        <property type="entry name" value="Hstdl_DH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21256">
        <property type="entry name" value="PTHR21256"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00815">
        <property type="entry name" value="Histidinol_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000099">
        <property type="entry name" value="Histidinol_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00083">
        <property type="entry name" value="HOLDHDRGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="SSF53720"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00069">
        <property type="entry name" value="hisD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00611">
        <property type="entry name" value="HISOL_DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="5 6 7" id="KW-0368">Histidine biosynthesis</keyword>
    <keyword evidence="2 6 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 7 9" id="KW-0520">NAD</keyword>
    <keyword evidence="4 6 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6 11" id="KW-0862">Zinc</keyword>
    <feature type="active site" description="Proton acceptor" evidence="6 8">
        <location>
            <position position="323"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="6 8">
        <location>
            <position position="324"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6 9">
        <location>
            <position position="127"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6 9">
        <location>
            <position position="189"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6 9">
        <location>
            <position position="212"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6 10">
        <location>
            <position position="235"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6 10 11">
        <location>
            <position position="257"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6 10 11">
        <location>
            <position position="260"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6 10">
        <location>
            <position position="324"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6 10 11">
        <location>
            <position position="357"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6 10">
        <location>
            <position position="411"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6 10 11">
        <location>
            <position position="416"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00016531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR000099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000099-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000099-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000099-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR000099-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU004175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AEF96123.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="425" mass="46909" checksum="166556F442188F3C" modified="2011-07-27" version="1">MIIKKIKDLTEEEKSKIINRNKMNLENIIPTVQEILKNVREKGDEALRYYTKKFDGVDIEDFKVSKEEIENAYNKIDYKVIEAIEKAAENIKEFHEMQLKNLKEWEIEKNGIKAGQILRPIENVGCYVPGGRAFYPSTVLMTVIPAKVAGCERVIITSPPTKEGEGNPATLVAGDIAKADEIYKVGGAQAIGAMAYGTETIPKVDIIVGPGNVFVTAAKKLVYGDVAIDFPAGPSEVLIIADENANEEYVALDFLAQAEHDPNASCVITTTSMEKANSIKNKIIEEMEKAERREIIEKALENSAILVGDLDECIEFSNEYAPEHLEIITKNPREVLKKIKHAGSIFLGEYAPVPVGDYASGTNHVLPTSACARMYSGLSVETFLKKPTIQELTKEGLKNIADIVITLAEAEGLFGHAEAVRRRIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>F6BC64</accession>
    <name>F6BC64_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Nitrogen regulatory protein P-II</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1738</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the P(II) protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97270.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799859.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1738"/>
    <dbReference type="EnsemblBacteria" id="AEF97270">
        <property type="protein sequence ID" value="AEF97270"/>
        <property type="gene ID" value="Metig_1738"/>
    </dbReference>
    <dbReference type="GeneID" id="10644612"/>
    <dbReference type="KEGG" id="mig:Metig_1738"/>
    <dbReference type="HOGENOM" id="CLU_082268_0_1_2"/>
    <dbReference type="OMA" id="KYIPKRM"/>
    <dbReference type="OrthoDB" id="113533at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030234">
        <property type="term" value="F:enzyme regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006808">
        <property type="term" value="P:regulation of nitrogen utilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002187">
        <property type="entry name" value="N-reg_PII"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011322">
        <property type="entry name" value="N-reg_PII-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015867">
        <property type="entry name" value="N-reg_PII/ATP_PRibTrfase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017918">
        <property type="entry name" value="N-reg_PII_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30115">
        <property type="entry name" value="PTHR30115"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00543">
        <property type="entry name" value="P-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00340">
        <property type="entry name" value="PIIGLNB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00938">
        <property type="entry name" value="P-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54913">
        <property type="entry name" value="SSF54913"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00638">
        <property type="entry name" value="PII_GLNB_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51343">
        <property type="entry name" value="PII_GLNB_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU003936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF97270.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="129" mass="14114" checksum="B331113B8352A405" modified="2011-07-27" version="1">MKEVVAIIRPKMVSKTGKALEAVGFPAMTVIECFGRGKQKGYIDVNLPECCVDLQKVIEEGEKEGRYIKYIPKRMISIVVEDADVPLVVGIIMKVNRTGNHGDGKIFVLPVEEAIRIRTGETGNAAIGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-09-29" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>F6BC79</accession>
    <name>F6BC79_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ATPase_2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1753</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97285.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799874.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF97285">
        <property type="protein sequence ID" value="AEF97285"/>
        <property type="gene ID" value="Metig_1753"/>
    </dbReference>
    <dbReference type="GeneID" id="10644627"/>
    <dbReference type="KEGG" id="mig:Metig_1753"/>
    <dbReference type="HOGENOM" id="CLU_068608_0_0_2"/>
    <dbReference type="OMA" id="PREYIVF"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011579">
        <property type="entry name" value="ATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01637">
        <property type="entry name" value="ATPase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATPase_2" evidence="1">
        <location>
            <begin position="3"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF97285.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="356" mass="42147" checksum="5FAD26567F285988" modified="2011-07-27" version="1">MEFFNREKKIKEILNILESEPNFIYFIFGPINSGKTTLINHIITKKLDKRYIPFYINFRRRAIINVDNFIECLFEVDEKSKIEDFREYAKSLSDLLSKSSETVTNYYLGMPKNFFEKKEKSGDIYQYIEYLFAELNKKGKIPILIFDELQMIKELTLNGKRPLLKSLFQFLVSLTKEQHLCHVFCLTSDSLFAEYVYNTGELGGRAKYLLVDDFDKETALKFMDFLSEDILKRKLTKEEKELIYSYVGGKPVDIIYVIENLKHKTLNNILNEMLNGEISKLSYFLEEIEENDKEVHNFLINALKLFKDNYIIDDRKIKKNVRELLITKNILFLNPTNRTIKPQSFLTWNAIKKVIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>F6BC90</accession>
    <name>F6BC90_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Zinc/iron permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Metig_1764</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97296.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799885.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1764"/>
    <dbReference type="EnsemblBacteria" id="AEF97296">
        <property type="protein sequence ID" value="AEF97296"/>
        <property type="gene ID" value="Metig_1764"/>
    </dbReference>
    <dbReference type="GeneID" id="10644638"/>
    <dbReference type="KEGG" id="mig:Metig_1764"/>
    <dbReference type="HOGENOM" id="CLU_1187785_0_0_2"/>
    <dbReference type="OMA" id="IHTFIDG"/>
    <dbReference type="OrthoDB" id="73105at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046873">
        <property type="term" value="F:metal ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003689">
        <property type="entry name" value="ZIP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02535">
        <property type="entry name" value="Zip"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="37"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="61"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="100"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="157"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="184"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="212"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEF97296.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="233" mass="25972" checksum="B8E8B36A6EACD2FE" modified="2011-07-27" version="1">MLNEAIFVSLLSFVFMLMGELMAYYSVSLKYRYEYEAFSYGFILGVATLVLIPVGHFENSSLYVILGVLFVFLLDKYLAFCPLCKKYCEECGVNYMQVKLLYPISFFIHTFIDGLIIAMGFIGHLGLLLYLAILLHKLPAGFVLLSPLKSIYKNPLLIGVIVSFGTVLGTIVGLLLFSNIPIKPLISISGGVFIATFLLLASHVYEHAPEKVINPLILGFLLVGLLSLLRHHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F6BC94</accession>
    <name>F6BC94_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="3">AAA ATPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_1768</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the archaeal ATPase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97300.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF97300">
        <property type="protein sequence ID" value="AEF97300"/>
        <property type="gene ID" value="Metig_1768"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_1768"/>
    <dbReference type="HOGENOM" id="CLU_068608_0_0_2"/>
    <dbReference type="OMA" id="WCERELT"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011579">
        <property type="entry name" value="ATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01637">
        <property type="entry name" value="ATPase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA" evidence="2">
        <location>
            <begin position="42"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF97300.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="370" mass="43673" checksum="956DA3B0121E8F68" modified="2011-07-27" version="1">MLMISTPTTKKNLLNLVGGETMNLFNREKEMKEILSIIEFEPNFIYFIYGPINSGKTALINEIINNRLDKDRYVVFYFDLREIFISKYDDFIEVLFEEYEEDKNPIELVKDLIKDSPILFGIPTPKNTLETLLSKKGPKNVFKYITNILMKIKESGKQPIIILDELQKIGDLKINGFLIYELFNYFISLTKHKHLCHVFCLSSDSLFIEKVYNEAILNGRAKYLLVDDFDKETSFKFMDFLSERVLNKKLSNEEKELIYSHVGGKLVDIIYIIQELKNKNLEEILDYLLKDAKQKLKYFLEDVKEENEELYNKVLESLKIFKENYEVEDITINKKVREFLVKKNILFLNPIEGTLRPQSFLIWNGIKKIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCA2</accession>
    <name>F6BCA2_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 8">ATP phosphoribosyltransferase</fullName>
            <shortName evidence="8">ATP-PRT</shortName>
            <shortName evidence="8">ATP-PRTase</shortName>
            <ecNumber evidence="3 8">2.4.2.17</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">hisG</name>
        <name evidence="11" type="ordered locus">Metig_1776</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the condensation of ATP and 5-phosphoribose 1-diphosphate to form N'-(5'-phosphoribosyl)-ATP (PR-ATP). Has a crucial role in the pathway because the rate of histidine biosynthesis seems to be controlled primarily by regulation of HisG enzymatic activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>1-(5-phospho-beta-D-ribosyl)-ATP + diphosphate = 5-phospho-alpha-D-ribose 1-diphosphate + ATP</text>
            <dbReference type="Rhea" id="RHEA:18473"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:73183"/>
            <dbReference type="EC" id="2.4.2.17"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="activity regulation">
        <text evidence="8">Feedback inhibited by histidine.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 8">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 1/9.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the ATP phosphoribosyltransferase family. Long subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.17" evidence="3 8"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97308.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1776"/>
    <dbReference type="EnsemblBacteria" id="AEF97308">
        <property type="protein sequence ID" value="AEF97308"/>
        <property type="gene ID" value="Metig_1776"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_1776"/>
    <dbReference type="HOGENOM" id="CLU_038115_1_1_2"/>
    <dbReference type="OMA" id="YVMMDYD"/>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00006"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003879">
        <property type="term" value="F:ATP phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00079">
        <property type="entry name" value="HisG_Long"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013820">
        <property type="entry name" value="ATP_PRibTrfase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018198">
        <property type="entry name" value="ATP_PRibTrfase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001348">
        <property type="entry name" value="ATP_PRibTrfase_HisG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020621">
        <property type="entry name" value="ATP_PRibTrfase_HisG_long"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013115">
        <property type="entry name" value="HisG_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011322">
        <property type="entry name" value="N-reg_PII-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015867">
        <property type="entry name" value="N-reg_PII/ATP_PRibTrfase_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21403">
        <property type="entry name" value="PTHR21403"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01634">
        <property type="entry name" value="HisG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08029">
        <property type="entry name" value="HisG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54913">
        <property type="entry name" value="SSF54913"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00070">
        <property type="entry name" value="hisG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03455">
        <property type="entry name" value="HisG_C-term"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01316">
        <property type="entry name" value="ATP_P_PHORIBOSYLTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 8 11" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="7 8" id="KW-0368">Histidine biosynthesis</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 8 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="HisG" evidence="9">
        <location>
            <begin position="52"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="domain" description="HisG_C" evidence="10">
        <location>
            <begin position="216"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF08029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AEF97308.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="291" mass="32268" checksum="35D08AF3B88C80C2" modified="2011-07-27" version="1">MIILILLALPNKGRISEPVNRILEKAGLKITVQGRSLFAQTVDEEIKVMFARARDIPEFVADGVADVGVTGYDLVLERGVEDKVEFLLDFNFGKARLVIAAPEDSDINSIDDLRDGMKIATEFPNLTRKYLKSKGLNLEIIELSGATEIAPFIGVADLISDLTSTGTTLRLNRLKIIDEIVSSTTRLIANKESLKDEKKRKKINQIVTGIKSVLYAETKRLIMMNAPKDKVEEIRKIMPGMSGPTISEVLSDKNMVAIHAVVDEKEVFSLVNKLEELGARDILVVPIERIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCB2</accession>
    <name>F6BCB2_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_1786</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97318.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1786"/>
    <dbReference type="EnsemblBacteria" id="AEF97318">
        <property type="protein sequence ID" value="AEF97318"/>
        <property type="gene ID" value="Metig_1786"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_1786"/>
    <dbReference type="HOGENOM" id="CLU_043239_0_0_2"/>
    <dbReference type="OMA" id="WVRGCYF"/>
    <dbReference type="OrthoDB" id="8710at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002708">
        <property type="entry name" value="HcyBio"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016426">
        <property type="entry name" value="MA1821-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01837">
        <property type="entry name" value="HcyBio"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004698">
        <property type="entry name" value="UCP004698_CBS_MJ0100"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="SSF54631"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0129">CBS domain</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBS" evidence="2">
        <location>
            <begin position="394"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="2">
        <location>
            <begin position="455"/>
            <end position="511"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF97318.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="511" mass="56197" checksum="33B4E174D4AF361B" modified="2011-07-27" version="1">MKTIKEINEKIRSGDVVVVTAEEMIGIVEDVGPEKAAEEVDVVTTGTFGAMCSSGVFLNFGHADPPIKMLKIYLNGVECYGGLAAVDTYIGATQPNSDPDIDIDYGGAHVIEDLVAGKEVELVAEGYTTDCYPRKKVRTTITLDDINQAILVNPRNSYQTYNAATNSRDEKIYTYMGTLLPEFGNVNYSGAGQLNPLQNDYNRKTKTYNTIGIGTRIFLGGGIGYIIGEGTQHNPDNAFGTLMVKGDLKQMDRKYLRAATIPNYGSTLFVGVGVPIPVLNAEVAKCCAIRDRDIEVPIIDYGVPRRDRPVVARTNYEELRKGKITIEVEIDGKKVEKTIKTSSVSSYKMSREIAKELKDWILKGEFFLTERVDVLGKGSAKPMKSDVKLVGEILSKPPIVAPIGITIEEASRILIKNNINHLPIVDEFGRLVGIITSWDIARAIAQNKKSIEEIMTRNVITTTVNEPVDVVARKMDKYNISGVPVVDKENKVVGMVTSEDLSKLVGYTNKW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCD8</accession>
    <name>F6BCD8_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Queuosine synthesis-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_0595</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96149.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798756.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0595"/>
    <dbReference type="EnsemblBacteria" id="AEF96149">
        <property type="protein sequence ID" value="AEF96149"/>
        <property type="gene ID" value="Metig_0595"/>
    </dbReference>
    <dbReference type="GeneID" id="10643433"/>
    <dbReference type="KEGG" id="mig:Metig_0595"/>
    <dbReference type="HOGENOM" id="CLU_1381418_0_0_2"/>
    <dbReference type="OMA" id="FYDVTLV"/>
    <dbReference type="OrthoDB" id="71195at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018317">
        <property type="entry name" value="QueC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06508">
        <property type="entry name" value="QueC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF96149.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="197" mass="22727" checksum="3E635D7E9EAA608F" modified="2011-07-27" version="1">MEAYVLFSGGKDSSLSAIILKKLGYDVKLLTVNFGILDSYKYAQETAEILGLPHEVITLEREILEKSVDIILKDGYPSNGIQFIHKKVLEILADKYKILADGTRRDDKVPKLSYSEVQSLEMRKNIEYLTPLMGLGHRTIRKLVNEYFIISEKESEELPKSDYETEIRALMRLKGYDPLKYFPRHKQSRVIGLKKEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCF2</accession>
    <name>F6BCF2_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">tRNA-guanine(15) transglycosylase</fullName>
            <ecNumber evidence="3">2.4.2.48</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">7-cyano-7-deazaguanine tRNA-ribosyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="3">Archaeal tRNA-guanine transglycosylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">tgtA</name>
        <name evidence="5" type="ordered locus">Metig_0611</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Exchanges the guanine residue with 7-cyano-7-deazaguanine (preQ0) at position 15 in the dihydrouridine loop (D-loop) of archaeal tRNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>7-cyano-7-deazaguanine + guanosine(15) in tRNA = 7-cyano-7-carbaguanosine(15) in tRNA + guanine</text>
            <dbReference type="Rhea" id="RHEA:43164"/>
            <dbReference type="Rhea" id="RHEA-COMP:10371"/>
            <dbReference type="Rhea" id="RHEA-COMP:10372"/>
            <dbReference type="ChEBI" id="CHEBI:16235"/>
            <dbReference type="ChEBI" id="CHEBI:45075"/>
            <dbReference type="ChEBI" id="CHEBI:74269"/>
            <dbReference type="ChEBI" id="CHEBI:82850"/>
            <dbReference type="EC" id="2.4.2.48"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="3">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 3">tRNA modification; archaeosine-tRNA biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the archaeosine tRNA-ribosyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.48" evidence="3"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96163.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798769.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0611"/>
    <dbReference type="EnsemblBacteria" id="AEF96163">
        <property type="protein sequence ID" value="AEF96163"/>
        <property type="gene ID" value="Metig_0611"/>
    </dbReference>
    <dbReference type="GeneID" id="10643450"/>
    <dbReference type="KEGG" id="mig:Metig_0611"/>
    <dbReference type="HOGENOM" id="CLU_030083_0_0_2"/>
    <dbReference type="OMA" id="FPCSCPV"/>
    <dbReference type="OrthoDB" id="10236at2157"/>
    <dbReference type="UniPathway" id="UPA00393"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016763">
        <property type="term" value="F:pentosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006400">
        <property type="term" value="P:tRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.130.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.105">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1020.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01634">
        <property type="entry name" value="TgtA_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038370">
        <property type="entry name" value="ArcTGT_C1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002478">
        <property type="entry name" value="PUA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036974">
        <property type="entry name" value="PUA_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036511">
        <property type="entry name" value="TGT-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029402">
        <property type="entry name" value="TGT_C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038250">
        <property type="entry name" value="TGT_C2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004804">
        <property type="entry name" value="TgtA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002616">
        <property type="entry name" value="tRNA_ribo_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004521">
        <property type="entry name" value="Uncharacterised_CHP00451"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01472">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01702">
        <property type="entry name" value="TGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14810">
        <property type="entry name" value="TGT_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00359">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51713">
        <property type="entry name" value="SSF51713"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="SSF88697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00432">
        <property type="entry name" value="arcsn_tRNA_tgt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00449">
        <property type="entry name" value="tgt_general"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00451">
        <property type="entry name" value="unchar_dom_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0808">Transferase</keyword>
    <keyword evidence="2 3" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="PUA" evidence="4">
        <location>
            <begin position="574"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="3">
        <location>
            <position position="88"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="123"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="194"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="280"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="282"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="285"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEF96163.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="651" mass="75598" checksum="08CDE0CE7C9B2EF3" modified="2011-07-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCH5</accession>
    <name>F6BCH5_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Amino acid permease-associated region</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Metig_0636</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96186.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798791.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0636"/>
    <dbReference type="EnsemblBacteria" id="AEF96186">
        <property type="protein sequence ID" value="AEF96186"/>
        <property type="gene ID" value="Metig_0636"/>
    </dbReference>
    <dbReference type="GeneID" id="10643475"/>
    <dbReference type="KEGG" id="mig:Metig_0636"/>
    <dbReference type="HOGENOM" id="CLU_743174_0_0_2"/>
    <dbReference type="OMA" id="FAYDGWL"/>
    <dbReference type="OrthoDB" id="15038at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002293">
        <property type="entry name" value="AA/rel_permease1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13520">
        <property type="entry name" value="AA_permease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="9"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="38"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="80"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="113"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="138"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="175"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="204"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="249"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="321"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="351"/>
            <end position="372"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEF96186.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="375" mass="41729" checksum="925A1921063D49DC" modified="2011-07-27" version="1">MKYLTLKDAVFLTITSIIGGGIFVLSPLTYLIAGKCAIYGWILVLMISMVLVLPFAYATTKITKSGGPYKYVMRIFGKKVGTIFAYTLWFAGVTAISAVVSFFSIIFNIYFNFEYVGIVLVIFLTLLIINGVKVVGNFLRIFGTLTVITLLFIIFSNKFDLSVFDAKVNLFKVLVTSYFGLWTMTGWEGISIPSEAFKNPERDIGYGLIIGTFIIGILYLLYTLVVISSNITYSELGDVIGALIGNNSLIWVCILLIIGGCVFSWLFSLGWMPYALSHDKIFIPKFSDAFVKLRKGIPTNGVLLNSFIIATLSIYPSKTLVDLGMFLTLISYFVLYLSVFKEKVSAFKIKFVSLLAAVITLLIIAFRVYLLFNGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCK8</accession>
    <name>F6BCK8_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="4">ABC-type transporter, periplasmic subunit family 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metig_0669</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96219.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798822.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0669"/>
    <dbReference type="EnsemblBacteria" id="AEF96219">
        <property type="protein sequence ID" value="AEF96219"/>
        <property type="gene ID" value="Metig_0669"/>
    </dbReference>
    <dbReference type="GeneID" id="10643509"/>
    <dbReference type="KEGG" id="mig:Metig_0669"/>
    <dbReference type="HOGENOM" id="CLU_019602_18_2_2"/>
    <dbReference type="OMA" id="VMGEAIN"/>
    <dbReference type="OrthoDB" id="56199at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015276">
        <property type="term" value="F:ligand-gated ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001320">
        <property type="entry name" value="Iontro_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001638">
        <property type="entry name" value="Solute-binding_3/MltF_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00497">
        <property type="entry name" value="SBP_bac_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00062">
        <property type="entry name" value="PBPb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00079">
        <property type="entry name" value="PBPe"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="domain" description="PBPb" evidence="2">
        <location>
            <begin position="39"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="domain" description="PBPe" evidence="3">
        <location>
            <begin position="41"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEF96219.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="263" mass="30140" checksum="B7143EBF1502A43D" modified="2011-07-27" version="1">MRWNLYLVCLSILLFFVVTCGCFENEGYEGEIKTIKPGVLTVGIVNYNVPPFVFEKDGKLTGFDVELITEISRRLGLKPEFKRYDFHLLLPLIKANEIDCAISAISITQKRKQEVSFSRPYFETGEIIVVRSDDNRIKRDADLRGKKVGVIKGTTCEELAISISKSAPLTIVKYRDVDKMLDDLKNGEIDAIIYDEILVDGVIKERPYFKKVGRRLTSEYYGIAVNKNNEALLNAINEALFEMEKDGTYMKLYNKWFGNFTQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCN6</accession>
    <name>F6BCN6_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Formylmethanofuran--tetrahydromethanopterin formyltransferase</fullName>
            <shortName evidence="3">Ftr</shortName>
            <ecNumber evidence="3">2.3.1.101</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">H4MPT formyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">ftr</name>
        <name evidence="6" type="ordered locus">Metig_0697</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the reversible transfer of a formyl group from formylmethanofuran (formyl-MFR) to tetrahydromethanopterin (H(4)MPT) to produce 5-formyl tetrahydromethanopterin (5-formyl-H(4)MPT) and methanofuran (MFR).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>5,6,7,8-tetrahydromethanopterin + H(+) + N-formylmethanofuran = methanofuran + N(5)-formyl-5,6,7,8-tetrahydromethanopterin</text>
            <dbReference type="Rhea" id="RHEA:18061"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57727"/>
            <dbReference type="ChEBI" id="CHEBI:58018"/>
            <dbReference type="ChEBI" id="CHEBI:58103"/>
            <dbReference type="ChEBI" id="CHEBI:58151"/>
            <dbReference type="EC" id="2.3.1.101"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">One-carbon metabolism; methanogenesis from CO(2); 5,10-methenyl-5,6,7,8-tetrahydromethanopterin from CO(2): step 2/3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the FTR family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.101" evidence="3"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96247.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798850.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0697"/>
    <dbReference type="EnsemblBacteria" id="AEF96247">
        <property type="protein sequence ID" value="AEF96247"/>
        <property type="gene ID" value="Metig_0697"/>
    </dbReference>
    <dbReference type="GeneID" id="10643537"/>
    <dbReference type="KEGG" id="mig:Metig_0697"/>
    <dbReference type="HOGENOM" id="CLU_081314_0_0_2"/>
    <dbReference type="OMA" id="VIMCPAE"/>
    <dbReference type="OrthoDB" id="62652at2157"/>
    <dbReference type="UniPathway" id="UPA00640">
        <property type="reaction ID" value="UER00693"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030270">
        <property type="term" value="F:formylmethanofuran-tetrahydromethanopterin N-formyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019386">
        <property type="term" value="P:methanogenesis, from carbon dioxide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.520">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00579">
        <property type="entry name" value="FTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014053">
        <property type="entry name" value="ForMFR_H4MPT_ForTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002770">
        <property type="entry name" value="ForMFR_H4MPT_ForTrfase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023447">
        <property type="entry name" value="ForMFR_H4MPT_ForTrfase_fd-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022667">
        <property type="entry name" value="ForMFR_H4MPT_ForTrfase_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01913">
        <property type="entry name" value="FTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02741">
        <property type="entry name" value="FTR_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006414">
        <property type="entry name" value="Ftr_formyl_trnsf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55112">
        <property type="entry name" value="SSF55112"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03119">
        <property type="entry name" value="one_C_fhcD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0484">Methanogenesis</keyword>
    <keyword evidence="3" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="FTR" evidence="4">
        <location>
            <begin position="1"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="domain" description="FTR_C" evidence="5">
        <location>
            <begin position="148"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00579"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEF96247.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="301" mass="32302" checksum="CD8B827C35B9711D" modified="2011-07-27" version="1">MEINGVIIEDTFAEAFPIWVSRILVTAATERLAKIAATEATGFGCSVIMCPAEAGIEKYVPPTETPDGRPGYIIQICHPKKSELEHQMLERIGQCILTAPTTAAFDAMGDEAEEQLKVGFKLKFFGDGYEKKDKLGDRTIYRIPIMSGEFITESKFGVKKGVAGGNFFILAENQTAALVAAEAAVDAISSVDGVITPFPGGIVASGSKVGASNPKYKFMTATTNHKMCPTLRDVVEDSEVPEDVNGIYEIVIDGISEEAVKEAMKVGILAATKVPGVKKITAGNYGGKLGKYQIKLHDLFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCN9</accession>
    <name>F6BCN9_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">FAD synthase</fullName>
            <ecNumber evidence="4">2.7.7.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">FMN adenylyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">Flavin adenine dinucleotide synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">ribL</name>
        <name evidence="6" type="ordered locus">Metig_0700</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Catalyzes the transfer of the AMP portion of ATP to flavin mononucleotide (FMN) to produce flavin adenine dinucleotide (FAD) coenzyme.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>ATP + FMN + H(+) = diphosphate + FAD</text>
            <dbReference type="Rhea" id="RHEA:17237"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="EC" id="2.7.7.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4">Cofactor biosynthesis; FAD biosynthesis; FAD from FMN: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0047 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the archaeal FAD synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.2" evidence="4"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96250.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0700"/>
    <dbReference type="EnsemblBacteria" id="AEF96250">
        <property type="protein sequence ID" value="AEF96250"/>
        <property type="gene ID" value="Metig_0700"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0700"/>
    <dbReference type="HOGENOM" id="CLU_989055_0_0_2"/>
    <dbReference type="UniPathway" id="UPA00277">
        <property type="reaction ID" value="UER00407"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003919">
        <property type="term" value="F:FMN adenylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006747">
        <property type="term" value="P:FAD biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046444">
        <property type="term" value="P:FMN metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.460">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02115">
        <property type="entry name" value="FAD_synth_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004821">
        <property type="entry name" value="Cyt_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024902">
        <property type="entry name" value="FAD_synth_RibL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001602">
        <property type="entry name" value="UPF0047"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035917">
        <property type="entry name" value="YjbQ-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01467">
        <property type="entry name" value="CTP_transf_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01894">
        <property type="entry name" value="UPF0047"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111038">
        <property type="entry name" value="SSF111038"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00125">
        <property type="entry name" value="cyt_tran_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00149">
        <property type="entry name" value="TIGR00149_YjbQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0274">FAD</keyword>
    <keyword evidence="4" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="2 4" id="KW-0288">FMN</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="CTP_transf_like" evidence="5">
        <location>
            <begin position="11"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="14"/>
            <end position="15"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="19"/>
            <end position="22"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="99"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="127"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEF96250.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="281" mass="32136" checksum="C8B578D3901904C8" modified="2011-07-27" version="1">MEKEKKKIVVTAGTFDLLHPGHYETLKFAKSLGDELIVIIARDETVKKIKGRKPVIPEEQRRRMVEALKPVDKAILGSLKDKLEPILKIKPDVIVLGPDQTTFDVEELKNELAKHNIFPEIVKVKEYTKCPFHSSFDIVKEIVRRFGGCKMIYSTLSINTNKRFEIIDITEKINKIIKESNVKNGLLNIFVPHTTAGLLINENEPNLIKDIENMLKELIKDEDYNHDKIDNNARAHLMSCLLKSDLTIPIIDGKILGTWQSILFVECDGPRSREVIVSIRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCP0</accession>
    <name>F6BCP0_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Metig_0701</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the autoinducer-2 exporter (AI-2E) (TC 2.A.86) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96251.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798854.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0701"/>
    <dbReference type="EnsemblBacteria" id="AEF96251">
        <property type="protein sequence ID" value="AEF96251"/>
        <property type="gene ID" value="Metig_0701"/>
    </dbReference>
    <dbReference type="GeneID" id="10643541"/>
    <dbReference type="KEGG" id="mig:Metig_0701"/>
    <dbReference type="HOGENOM" id="CLU_041771_3_0_2"/>
    <dbReference type="OMA" id="EIAYVYQ"/>
    <dbReference type="OrthoDB" id="79655at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002549">
        <property type="entry name" value="AI-2E-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21716">
        <property type="entry name" value="PTHR21716"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01594">
        <property type="entry name" value="AI-2E_transport"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="52"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="136"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="193"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="221"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="255"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="288"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEF96251.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="333" mass="37769" checksum="E99B001A1B7CB0A3" modified="2011-07-27" version="1">MSIEEFKLLKRIIAIAVFLSCFYMVWPFIDVLAFSCAFAYMTKPIYDRLLPYLGKSLSALVCLLLFTIPTVILMLVVLKEILLYVQTIDTNAITNNILILLNYFGINRTSVGEFNQLLVQAWEFIKPSIKSMINQISLLPHIVIKIVMIFFTTYYFLKDGYKLKDAILYHVPEEYQYKTKIFLEYLNESYKNLFIGNAFTSLIIGIIAGIGYYLIGVPNALFLAILTGLFALLPIVGGWTVYIPLTIYYIISEDYIKALEIFIFGAVFLSTLPDFVIRPFVVKKEADLHPVLILIAFLIGPLTLGIKGFALGPMIVGALNAIFATKIKRKVEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCR3</accession>
    <name>F6BCR3_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Histone deacetylase superfamily</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0724</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96274.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798877.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0724"/>
    <dbReference type="EnsemblBacteria" id="AEF96274">
        <property type="protein sequence ID" value="AEF96274"/>
        <property type="gene ID" value="Metig_0724"/>
    </dbReference>
    <dbReference type="GeneID" id="10643564"/>
    <dbReference type="KEGG" id="mig:Metig_0724"/>
    <dbReference type="HOGENOM" id="CLU_007727_8_1_2"/>
    <dbReference type="OMA" id="CTSPAMG"/>
    <dbReference type="OrthoDB" id="34461at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.800.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000286">
        <property type="entry name" value="His_deacetylse"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023801">
        <property type="entry name" value="His_deacetylse_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037138">
        <property type="entry name" value="His_deacetylse_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023696">
        <property type="entry name" value="Ureohydrolase_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00850">
        <property type="entry name" value="Hist_deacetyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01270">
        <property type="entry name" value="HDASUPER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52768">
        <property type="entry name" value="SSF52768"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Hist_deacetyl" evidence="1">
        <location>
            <begin position="90"/>
            <end position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96274.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="277" mass="31324" checksum="BC589893029538D4" modified="2011-07-27" version="1">MKVVFPKDCIKYCIEGHPESPKRVELIYNALMDKVEFVDAKPAKKEDILLCHTEEHYNKVLNKDYFDWDTPVIEPYYPLLSAGSAIKAAEINGFSLSRPPGHHAGSDFLEGFCYFNNICIATKKLGKRTAILDLDVHHGNGTEDIVFGDEDILYVSIHQHPLYPGTGLKSEENCINFPLPPLTDEDLYLKTLKKALEHVVAFEPEVLAISLGFDTYIGDPLANFALKEESYEKIGKLVGDVVEEYNFNYFIVLEGGYSDKIGDLALKFFEGFVWSRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCU9</accession>
    <name>F6BCU9_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0764</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0047 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96310.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798913.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0764"/>
    <dbReference type="EnsemblBacteria" id="AEF96310">
        <property type="protein sequence ID" value="AEF96310"/>
        <property type="gene ID" value="Metig_0764"/>
    </dbReference>
    <dbReference type="GeneID" id="10643604"/>
    <dbReference type="KEGG" id="mig:Metig_0764"/>
    <dbReference type="HOGENOM" id="CLU_096980_1_1_2"/>
    <dbReference type="OMA" id="VRHTSCS"/>
    <dbReference type="OrthoDB" id="85388at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.460">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001602">
        <property type="entry name" value="UPF0047"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035917">
        <property type="entry name" value="YjbQ-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30615">
        <property type="entry name" value="PTHR30615"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01894">
        <property type="entry name" value="UPF0047"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004681">
        <property type="entry name" value="UCP004681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111038">
        <property type="entry name" value="SSF111038"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00149">
        <property type="entry name" value="TIGR00149_YjbQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01314">
        <property type="entry name" value="UPF0047"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96310.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="132" mass="14962" checksum="7764EFC4C059B913" modified="2011-07-27" version="1">MLFKYSIKTNSREEMIDITSYVEESVKKANVKEGVVVVFTPHTTSAITINENADPSVRRDIIAFLKDKIPRDYPFTHIEGNSDAHIKSSTFGCSLTVIITNGKLLLGTWQGIYFCEFDGPRHRNFYVKVIEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCV0</accession>
    <name>F6BCV0_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 6">2-(3-amino-3-carboxypropyl)histidine synthase</fullName>
            <ecNumber evidence="3 6">2.5.1.108</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Metig_0765</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes the first step of diphthamide biosynthesis, i.e. the transfer of the 3-amino-3-carboxypropyl group from S-adenosyl-L-methionine (SAM) to the C2 position of the imidazole ring of the target histidine residue in translation elongation factor 2 (EF-2).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 6">
            <text>L-histidyl-[translation elongation factor 2] + S-adenosyl-L-methionine = 2-[(3S)-amino-3-carboxypropyl]-L-histidyl-[translation elongation factor 2] + H(+) + S-methyl-5'-thioadenosine</text>
            <dbReference type="Rhea" id="RHEA:36783"/>
            <dbReference type="Rhea" id="RHEA-COMP:9748"/>
            <dbReference type="Rhea" id="RHEA-COMP:9749"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17509"/>
            <dbReference type="ChEBI" id="CHEBI:29979"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:73995"/>
            <dbReference type="EC" id="2.5.1.108"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2 6">Protein modification; peptidyl-diphthamide biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the DPH1/DPH2 family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.108" evidence="3 6"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96311.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798914.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0765"/>
    <dbReference type="EnsemblBacteria" id="AEF96311">
        <property type="protein sequence ID" value="AEF96311"/>
        <property type="gene ID" value="Metig_0765"/>
    </dbReference>
    <dbReference type="GeneID" id="10643605"/>
    <dbReference type="KEGG" id="mig:Metig_0765"/>
    <dbReference type="HOGENOM" id="CLU_037146_0_0_2"/>
    <dbReference type="OMA" id="PGQVLGC"/>
    <dbReference type="OrthoDB" id="87520at2157"/>
    <dbReference type="UniPathway" id="UPA00559"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090560">
        <property type="term" value="F:2-(3-amino-3-carboxypropyl)histidine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017183">
        <property type="term" value="P:peptidyl-diphthamide biosynthetic process from peptidyl-histidine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11840">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11850">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11860">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016435">
        <property type="entry name" value="DPH1/DPH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042263">
        <property type="entry name" value="DPH1/DPH2_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042264">
        <property type="entry name" value="DPH1/DPH2_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042265">
        <property type="entry name" value="DPH1/DPH2_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035435">
        <property type="entry name" value="DPH1/DPH2_euk_archaea"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022428">
        <property type="entry name" value="Dph2_arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10762">
        <property type="entry name" value="PTHR10762"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01866">
        <property type="entry name" value="Diphthamide_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004967">
        <property type="entry name" value="DPH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00032">
        <property type="entry name" value="Radical_SAM_3-amino-3-carboxyp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03682">
        <property type="entry name" value="arCOG04112"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00322">
        <property type="entry name" value="diphth2_R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4 6" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR004967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEF96311.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="319" mass="36761" checksum="994FDBDD756BE535" modified="2011-07-27" version="1">MWNLETERIINEIEKRNAKRILFQAPEGLKKDVEKEIEKIKKYFDEKGKSLELMIWGGTCFGGCDLVDNEVKHLGIDLIIHYGHEELEYAKPSIPTIFVPVYHIFDEEEKNEILKDIEDIIKEKNITTVATTIQYKELLKKYNPTIILGCRAEINKEGNVLFVGTGRFHPLMIAYKIKKSVTIYNPATREISSIEEDEINKLIKRRIGIISKLVINPPKKIGIVLSTKKGQCRKKIFEYVKDLLKNAGIDYISIVVNSLSPQSLIYDVDAYVIVACPRIVMDDSALYDKPILTPKELEMLIKGEFEYVFDEIKKEDFEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCX8</accession>
    <name>F6BCX8_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Anaerobic ribonucleoside-triphosphate reductase activating protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Metig_0794</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96339.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0794"/>
    <dbReference type="EnsemblBacteria" id="AEF96339">
        <property type="protein sequence ID" value="AEF96339"/>
        <property type="gene ID" value="Metig_0794"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0794"/>
    <dbReference type="HOGENOM" id="CLU_078147_2_1_2"/>
    <dbReference type="OMA" id="MDIKAPL"/>
    <dbReference type="OrthoDB" id="101349at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012840">
        <property type="entry name" value="NrdG2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01094">
        <property type="entry name" value="Uncharacterised_Radical_SAM_Su"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02495">
        <property type="entry name" value="NrdG2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="5">
        <location>
            <begin position="30"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEF96339.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="252" mass="29250" checksum="5B83523E0BE82A00" modified="2011-07-27" version="1">MLYKFYTYALNGENMEKIKVSGIVELSTIDYPKKCSAVIFLSDCNMKCKYCHNYEHITKNVYEMSAEEVFNDIDFLFAEAIVISGGEPTLQPKAIMELAKLAKSRGFPVKIDTNGTHPKVIEELIKNNLVDYVALDVKCRFDKYKELTEYDGDIKEKILRIINMCRENDVFIECRTTYIPTVMDKDDIEEIAKTVRNCNLYAIQQFDNEHAYDDEFKKIQPPKTKRLIELGNIAKKYIDNVVVRTFEGEIKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCY0</accession>
    <name>F6BCY0_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Citrate lyase ligase domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0796</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96341.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798944.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0796"/>
    <dbReference type="EnsemblBacteria" id="AEF96341">
        <property type="protein sequence ID" value="AEF96341"/>
        <property type="gene ID" value="Metig_0796"/>
    </dbReference>
    <dbReference type="GeneID" id="10643636"/>
    <dbReference type="KEGG" id="mig:Metig_0796"/>
    <dbReference type="HOGENOM" id="CLU_777585_0_0_2"/>
    <dbReference type="OMA" id="MGSGQYM"/>
    <dbReference type="OrthoDB" id="41648at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032266">
        <property type="entry name" value="HIGH_NTase1_ass"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008513">
        <property type="entry name" value="tRNA(Met)_cyd_acetate_ligase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37825">
        <property type="entry name" value="PTHR37825"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05636">
        <property type="entry name" value="HIGH_NTase1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16581">
        <property type="entry name" value="HIGH_NTase1_ass"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="2" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HIGH_NTase1_ass" evidence="1">
        <location>
            <begin position="141"/>
            <end position="343"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF16581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96341.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="347" mass="40241" checksum="300F1AF91668CF4D" modified="2011-07-27" version="1">MLKDFLKDREEIIKDAKAKDTKSFDEFKKIVEKLKERKGTDTIVADFTEYNPLHKGHKYCMDVGKRHGIFIAVVPGPLERSGRGIPYLVSRYIRAEMAIRAGADIVVEGPPMGIMGSGQYMQCLVKMFYKLNADIIPRGYIPEKTMEKVIDCINKGYHVAPKPYKIVCIETGEILGEKLGIDNYVIASMSKTIYKLNKLGEINFNPKFVFVRRLEGISGTKIREYIFSGDFEKAKEMLPPTTLEILKEYYDKGKLKDIILSRFEDRILETANEFELEKYLPEKIAKELEKHRPYESLDEIREAIPYGFSRHNKERILSKLEARIEKEIISEYVKNYPAKIRILYRSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCY3</accession>
    <name>F6BCY3_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">2-phosphoglycerate kinase</fullName>
            <shortName evidence="3">2PGK</shortName>
            <ecNumber evidence="3">2.7.2.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">pgk2</name>
        <name evidence="6" type="ordered locus">Metig_0799</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the phosphorylation of 2-phosphoglycerate to 2,3-diphosphoglycerate. Involved in the biosynthesis of cyclic 2,3-bisphosphoglycerate, a thermoprotectant.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>(2R)-2-phosphoglycerate + ATP = (2R)-2,3-bisphosphoglycerate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:42408"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58248"/>
            <dbReference type="ChEBI" id="CHEBI:58289"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.2.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Thermoadapter biosynthesis; cyclic 2,3-diphosphoglycerate biosynthesis; cyclic 2,3-diphosphoglycerate from 2-phospho-D-glycerate: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the 2-phosphoglycerate kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.2.16" evidence="3"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96344.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798947.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0799"/>
    <dbReference type="EnsemblBacteria" id="AEF96344">
        <property type="protein sequence ID" value="AEF96344"/>
        <property type="gene ID" value="Metig_0799"/>
    </dbReference>
    <dbReference type="GeneID" id="10643640"/>
    <dbReference type="KEGG" id="mig:Metig_0799"/>
    <dbReference type="HOGENOM" id="CLU_848909_0_0_2"/>
    <dbReference type="OMA" id="EMPFSKG"/>
    <dbReference type="OrthoDB" id="57277at2157"/>
    <dbReference type="UniPathway" id="UPA00551">
        <property type="reaction ID" value="UER00609"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016774">
        <property type="term" value="F:phosphotransferase activity, carboxyl group as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00769">
        <property type="entry name" value="2PGK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020872">
        <property type="entry name" value="2PKG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005144">
        <property type="entry name" value="ATP-cone_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03477">
        <property type="entry name" value="ATP-cone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51161">
        <property type="entry name" value="ATP_CONE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 6" id="KW-0418">Kinase</keyword>
    <keyword evidence="1 3 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="ATP-cone" evidence="5">
        <location>
            <begin position="8"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEF96344.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="307" mass="35115" checksum="9F9BFA6F869EF0AB" modified="2011-07-27" version="1">MGFEYLTNQIIVKDKDYEMPFSKGILARSLTAAGLKPSIAYSIAREIEGMLKDEGVKVISKDELRRRVYYYLIDKNYYHIAEKYLLWRRILKKHPIIILIGGASGVGTSTIAFEIASRLGIPSVIGTDSIREVMRRSISKDLVPMLYESSYTAWKAIRTPLPENCNIYITGFERHIEPVLIGVEGIIDRSLTEGLSVIIEGTHLVPGFISEKYTTMPNIIFIVLSLSSEELHKKRFSARARVSNRPMERYLKHFKIIRMINDYIVEQAKKNNVPVVENVSISQTVEKCLEIITERFMNLDDGNDDDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>F6BD19</accession>
    <name>F6BD19_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="3">CBS domain containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_0835</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96380.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798982.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0835"/>
    <dbReference type="EnsemblBacteria" id="AEF96380">
        <property type="protein sequence ID" value="AEF96380"/>
        <property type="gene ID" value="Metig_0835"/>
    </dbReference>
    <dbReference type="GeneID" id="10643676"/>
    <dbReference type="KEGG" id="mig:Metig_0835"/>
    <dbReference type="HOGENOM" id="CLU_982154_0_0_2"/>
    <dbReference type="OMA" id="GRIMFRT"/>
    <dbReference type="OrthoDB" id="46801at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003115">
        <property type="entry name" value="ParB/Sulfiredoxin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036086">
        <property type="entry name" value="ParB/Sulfiredoxin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016427">
        <property type="entry name" value="UCP004699_CBS/ParB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02195">
        <property type="entry name" value="ParBc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004699">
        <property type="entry name" value="UCP004699_CBS_ParB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00470">
        <property type="entry name" value="ParB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110849">
        <property type="entry name" value="SSF110849"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="SSF54631"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0129">CBS domain</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBS" evidence="2">
        <location>
            <begin position="8"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="2">
        <location>
            <begin position="66"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF96380.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="263" mass="29577" checksum="699C0A51996D34C6" modified="2011-07-27" version="1">MVKVSEYMTKKVDCLSPDNTVKDAIRFVKETGHDAFPVVVDGKLVGIVSVHDLVGKDENEKIGNIMTKREDMIVAKPDADIMDIGRLMFRTGFSKLPVVDDENNLVGIITNTDVIRSQIEKTTPEKLNKIVKTYENLGLKTRVERGYVSIDELKPTQSKVHEDELTGRMYELKKGLAEPIIAIKTKKSGYILIDGHHRAVAAYKLGIPKLDAYIIYIDTDKKLGIEKTAESQGLRTLKDIKIIDENNENSINTYTLKPKVNFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F6BD46</accession>
    <name>F6BD46_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">50S ribosomal protein L18e</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">rpl18e</name>
        <name evidence="6" type="ordered locus">Metig_0863</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein eL18 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96407.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799009.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0863"/>
    <dbReference type="EnsemblBacteria" id="AEF96407">
        <property type="protein sequence ID" value="AEF96407"/>
        <property type="gene ID" value="Metig_0863"/>
    </dbReference>
    <dbReference type="GeneID" id="10643706"/>
    <dbReference type="KEGG" id="mig:Metig_0863"/>
    <dbReference type="HOGENOM" id="CLU_146465_0_0_2"/>
    <dbReference type="OMA" id="WRLERPR"/>
    <dbReference type="OrthoDB" id="104254at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00329">
        <property type="entry name" value="Ribosomal_L18e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036227">
        <property type="entry name" value="L18e/L15P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001196">
        <property type="entry name" value="Ribosomal_L15_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000039">
        <property type="entry name" value="Ribosomal_L18e"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021131">
        <property type="entry name" value="Ribosomal_L18e/L15P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022947">
        <property type="entry name" value="Ribosomal_L18e_arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10934">
        <property type="entry name" value="PTHR10934"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17135">
        <property type="entry name" value="Ribosomal_L18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52080">
        <property type="entry name" value="SSF52080"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00475">
        <property type="entry name" value="RIBOSOMAL_L15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 6" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Ribosomal_L18e/L15P" evidence="5">
        <location>
            <begin position="3"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF17135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEF96407.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="120" mass="13209" checksum="D99F906EC3004489" modified="2011-07-27" version="1">MKKIRATNPRLVALIQKLKEEAYKNNAKIWKDIARRLAKPARNRAEVNVSKINRYTKEGDVVVVPGKVLGAGTINHKVTVAAFAFSKTAKQIIEAAGGRCITIEELVKENPKGSNVKIMA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>F6BD78</accession>
    <name>F6BD78_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">50S ribosomal protein L15</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">rpl15</name>
        <name evidence="8" type="ordered locus">Metig_0896</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Binds to the 23S rRNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4 5">Belongs to the universal ribosomal protein uL15 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96439.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799041.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0896"/>
    <dbReference type="EnsemblBacteria" id="AEF96439">
        <property type="protein sequence ID" value="AEF96439"/>
        <property type="gene ID" value="Metig_0896"/>
    </dbReference>
    <dbReference type="GeneID" id="10643740"/>
    <dbReference type="KEGG" id="mig:Metig_0896"/>
    <dbReference type="HOGENOM" id="CLU_109163_0_0_2"/>
    <dbReference type="OMA" id="WGRVGQH"/>
    <dbReference type="OrthoDB" id="121171at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015934">
        <property type="term" value="C:large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.990.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01341">
        <property type="entry name" value="Ribosomal_L15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036227">
        <property type="entry name" value="L18e/L15P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027386">
        <property type="entry name" value="Rbsml_prot_L15/27a_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030878">
        <property type="entry name" value="Ribosomal_L15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001196">
        <property type="entry name" value="Ribosomal_L15_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021131">
        <property type="entry name" value="Ribosomal_L18e/L15P"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00828">
        <property type="entry name" value="Ribosomal_L27A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52080">
        <property type="entry name" value="SSF52080"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00475">
        <property type="entry name" value="RIBOSOMAL_L15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 5" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="4" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="Ribosomal_L18e/L15P" evidence="7">
        <location>
            <begin position="29"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="13"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="13"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01341"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEF96439.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="143" mass="16038" checksum="4A88D41B3AACFC93" modified="2011-07-27" version="1">MIRKKKKIKKIRGSRTCGYGSHKKHRGAGSRGGRGRAGGFDHKWSYVIKYEPDRFGKYGFSRHPSLIKDLETINVGELEELVLKNKDKFEVEDGKIVVDVTELGFEKVLGRGKISTPMIVKAIEFSESAREKIESAGGEVVEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>F6BD88</accession>
    <name>F6BD88_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 7">30S ribosomal protein S4e</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">rps4e</name>
        <name evidence="10" type="ordered locus">Metig_0906</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the eukaryotic ribosomal protein eS4 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96449.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799051.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0906"/>
    <dbReference type="EnsemblBacteria" id="AEF96449">
        <property type="protein sequence ID" value="AEF96449"/>
        <property type="gene ID" value="Metig_0906"/>
    </dbReference>
    <dbReference type="GeneID" id="10643750"/>
    <dbReference type="KEGG" id="mig:Metig_0906"/>
    <dbReference type="HOGENOM" id="CLU_060400_0_0_2"/>
    <dbReference type="OMA" id="FPVGLMD"/>
    <dbReference type="OrthoDB" id="89943at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06087">
        <property type="entry name" value="KOW_RPS4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00165">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.740">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.290.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00485">
        <property type="entry name" value="Ribosomal_S4e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005824">
        <property type="entry name" value="KOW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041982">
        <property type="entry name" value="KOW_RPS4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_L2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000876">
        <property type="entry name" value="Ribosomal_S4e"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013845">
        <property type="entry name" value="Ribosomal_S4e_central_region"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038237">
        <property type="entry name" value="Ribosomal_S4e_central_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013843">
        <property type="entry name" value="Ribosomal_S4e_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018199">
        <property type="entry name" value="Ribosomal_S4e_N_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002942">
        <property type="entry name" value="S4_RNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036986">
        <property type="entry name" value="S4_RNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11581">
        <property type="entry name" value="PTHR11581"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00900">
        <property type="entry name" value="Ribosomal_S4e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08071">
        <property type="entry name" value="RS4NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01479">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002116">
        <property type="entry name" value="Ribosomal_S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00739">
        <property type="entry name" value="KOW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00363">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00528">
        <property type="entry name" value="RIBOSOMAL_S4E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 7 10" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3 7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="S4" evidence="8">
        <location>
            <begin position="45"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="domain" description="KOW" evidence="9">
        <location>
            <begin position="181"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00029531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AEF96449.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="244" mass="27783" checksum="0E6594599FA22079" modified="2011-07-27" version="1">MANKGPRRHLKRLAAPANWQIPRKVHKFVVRPLPGPHAMNESLPLLLIVRDILKYADNAREAKKIIKMGKILVDGRKRKEHKFPVGLMDVVSIPDTNEHFRALFDEKGRIVLRPTENPDVKLCRIRNKTVVKGGHIQLNLHDGRNHLIRVSDPTKAEEDVYKTGDVILMGIPEQEIKAHIPFEVGKLAYITGGKHIGEFAKIVDIEKRILYPDIITLENADGEKFKTIKDYVFVVGDEEPIIKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDA9</accession>
    <name>F6BDA9_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Methyl-viologen-reducing hydrogenase delta subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Metig_0927</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>2Fe-2S cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49601"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MvhD/VhuD family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96470.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0927"/>
    <dbReference type="KEGG" id="mig:Metig_0927"/>
    <dbReference type="HOGENOM" id="CLU_095272_2_0_2"/>
    <dbReference type="OMA" id="XGYGAAD"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003813">
        <property type="entry name" value="MvhD/FlpD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02662">
        <property type="entry name" value="FlpD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="5" id="KW-0249">Electron transport</keyword>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0711">Selenium</keyword>
    <keyword evidence="8" id="KW-0712">Selenocysteine</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="FlpD" evidence="7">
        <location>
            <begin position="6"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="non-standard amino acid" description="Selenocysteine" evidence="8">
        <location>
            <position position="14"/>
        </location>
    </feature>
    <feature type="non-standard amino acid" description="Selenocysteine" evidence="8">
        <location>
            <position position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEF96470.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="135" mass="15003" checksum="E074D0FC90A84457" modified="2011-07-27" version="1">MSEPIIVAFVCYQUGYGAADLAGTSRMQYPASVRPIRIPCTGKFDITYALRAFQKGADAVFVAGUKPHECAFETGNFRAEERVRFTKKLLDELGIGGERIEMYFMTAAEADKFVAAVNEMTERVKKLGPNPLKAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDC0</accession>
    <name>F6BDC0_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Tyrosine--tRNA ligase</fullName>
            <ecNumber evidence="8">6.1.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Tyrosyl-tRNA synthetase</fullName>
            <shortName evidence="8">TyrRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">tyrS</name>
        <name evidence="10" type="ordered locus">Metig_0938</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>ATP + L-tyrosine + tRNA(Tyr) = AMP + diphosphate + H(+) + L-tyrosyl-tRNA(Tyr)</text>
            <dbReference type="Rhea" id="RHEA:10220"/>
            <dbReference type="Rhea" id="RHEA-COMP:9706"/>
            <dbReference type="Rhea" id="RHEA-COMP:9707"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58315"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78536"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the class-I aminoacyl-tRNA synthetase family. TyrS type 3 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.1" evidence="8"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96481.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799083.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0938"/>
    <dbReference type="EnsemblBacteria" id="AEF96481">
        <property type="protein sequence ID" value="AEF96481"/>
        <property type="gene ID" value="Metig_0938"/>
    </dbReference>
    <dbReference type="GeneID" id="10643784"/>
    <dbReference type="KEGG" id="mig:Metig_0938"/>
    <dbReference type="HOGENOM" id="CLU_035267_0_1_2"/>
    <dbReference type="OMA" id="YIGFEIS"/>
    <dbReference type="OrthoDB" id="59062at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004831">
        <property type="term" value="F:tyrosine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006437">
        <property type="term" value="P:tyrosyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00805">
        <property type="entry name" value="TyrRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02008">
        <property type="entry name" value="Tyr_tRNA_synth_type3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002305">
        <property type="entry name" value="aa-tRNA-synth_Ic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002307">
        <property type="entry name" value="Tyr-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023684">
        <property type="entry name" value="Tyr-tRNA-ligase_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023617">
        <property type="entry name" value="Tyr-tRNA-ligase_arc/euk-type"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00579">
        <property type="entry name" value="tRNA-synt_1b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006588">
        <property type="entry name" value="TyrRS_arch_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01040">
        <property type="entry name" value="TRNASYNTHTYR"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00234">
        <property type="entry name" value="tyrS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8 9" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="5 8 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 8 9" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 8 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 8 9" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="short sequence motif" description="'HIGH' region" evidence="8">
        <location>
            <begin position="35"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'KMSKS' region" evidence="8">
        <location>
            <begin position="206"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="30"/>
        </location>
        <ligand>
            <name>L-tyrosine</name>
            <dbReference type="ChEBI" id="CHEBI:58315"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="149"/>
        </location>
        <ligand>
            <name>L-tyrosine</name>
            <dbReference type="ChEBI" id="CHEBI:58315"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="153"/>
        </location>
        <ligand>
            <name>L-tyrosine</name>
            <dbReference type="ChEBI" id="CHEBI:58315"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="156"/>
        </location>
        <ligand>
            <name>L-tyrosine</name>
            <dbReference type="ChEBI" id="CHEBI:58315"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="171"/>
        </location>
        <ligand>
            <name>L-tyrosine</name>
            <dbReference type="ChEBI" id="CHEBI:58315"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="209"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU363036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AEF96481.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="309" mass="35211" checksum="801D32B45C76EF41" modified="2011-07-27" version="1">MIDDILKNTLEVVSVEELKEVMEKKDKRAYIGFEPSGKIHLGHYLQIRKMIDLQNAGFDVVILLADLHAYLNQKGTMEEIKELAEYNKRCFEAMGVKAEYVYGSEFQLDKEYTLDVYKLALKTTLKRARRSMEVIAREDENPKVAEVIYPIMQVNDIKHLNVDVAVGGMEQRKIHMLARELLPSLGYKAPVCIHNPVLTGLDGEGKMSSSKGNFIAVDDEPDVIKSKIKKAYCPAKVVEGNPIMEIAKYFLEYPLTIKRPEKYGGDLVVNSYEELEKIFTEGKLHPMDLKNAVAEELIEMLSPIREKLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDD9</accession>
    <name>F6BDD9_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">DNA-directed RNA polymerase subunit Rpo12</fullName>
            <ecNumber evidence="8">2.7.7.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">DNA-directed RNA polymerase subunit P</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">rpo12</name>
        <name evidence="8" type="synonym">rpoP</name>
        <name evidence="9" type="ordered locus">Metig_0957</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">DNA-dependent RNA polymerase (RNAP) catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + RNA(n+1)</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="8">Binds 1 zinc ion.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Part of the RNA polymerase complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the archaeal Rpo12/eukaryotic RPC10 RNA polymerase subunit family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="8"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96500.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799102.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0957"/>
    <dbReference type="EnsemblBacteria" id="AEF96500">
        <property type="protein sequence ID" value="AEF96500"/>
        <property type="gene ID" value="Metig_0957"/>
    </dbReference>
    <dbReference type="GeneID" id="10643803"/>
    <dbReference type="KEGG" id="mig:Metig_0957"/>
    <dbReference type="HOGENOM" id="CLU_179456_2_1_2"/>
    <dbReference type="OrthoDB" id="129924at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed 5'-3' RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00615">
        <property type="entry name" value="RNApol_arch_Rpo12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006591">
        <property type="entry name" value="RNAP_P/RPABC4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029040">
        <property type="entry name" value="RPABC4/Spt4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023464">
        <property type="entry name" value="Rpo12"/>
    </dbReference>
    <dbReference type="SMART" id="SM00659">
        <property type="entry name" value="RPOLCX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63393">
        <property type="entry name" value="SSF63393"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1 8" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="5 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 8" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 8" id="KW-0804">Transcription</keyword>
    <keyword evidence="3 8" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 8" id="KW-0862">Zinc</keyword>
    <feature type="binding site" evidence="8">
        <location>
            <position position="9"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="24"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="27"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00615"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AEF96500.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="46" mass="5229" checksum="CDB088EAADB2E28C" modified="2011-07-27" version="1">MTEYKCINCKKVVTLDEIGSKAKCPYCSSKILIKLRPKTVKLVKAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-06-02" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDK4</accession>
    <name>F6BDK4_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_1023</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96565.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799167.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96565">
        <property type="protein sequence ID" value="AEF96565"/>
        <property type="gene ID" value="Metig_1023"/>
    </dbReference>
    <dbReference type="GeneID" id="10643877"/>
    <dbReference type="KEGG" id="mig:Metig_1023"/>
    <dbReference type="HOGENOM" id="CLU_2067823_0_0_2"/>
    <dbReference type="OrthoDB" id="115839at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF96565.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="118" mass="13566" checksum="60A22E261FD317C0" modified="2011-07-27" version="1">MDKIKITFENPKGIIISYINIKSSKLKEGDITLELLSEEVQSIEIPYNKDIEEGEITIKIKYKPFFEIGGKDIDIDDKNRDESVNVVNDVEYAKENEDNIIIDNDDEEDDNDVEIIIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDN3</accession>
    <name>F6BDN3_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Metig_1053</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the UPF0718 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96594.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799195.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1053"/>
    <dbReference type="EnsemblBacteria" id="AEF96594">
        <property type="protein sequence ID" value="AEF96594"/>
        <property type="gene ID" value="Metig_1053"/>
    </dbReference>
    <dbReference type="GeneID" id="10643907"/>
    <dbReference type="KEGG" id="mig:Metig_1053"/>
    <dbReference type="HOGENOM" id="CLU_975256_0_0_2"/>
    <dbReference type="OMA" id="TSLSFMM"/>
    <dbReference type="OrthoDB" id="76288at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005524">
        <property type="entry name" value="DUF318"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03773">
        <property type="entry name" value="ArsP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="17"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="58"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="91"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="118"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="167"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="198"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="234"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="263"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006386"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AEF96594.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="285" mass="31441" checksum="2CE873651E715EEC" modified="2011-07-27" version="1">MIDIYDIYLSLERSLNFFLRALPYLMLGFILSTYLKIKLKGDLRKKFVHVLDGSKKSIVLASFIGAILPLCSASGIPVASAMNSKGANLGVSFAFMVSASSINPIGILLTISLLGYKMVIVQVLASIVLSIFVGFIFYNEKAIFCGFETKNHNNSFFSAFIEQFRKLFPSIVLGFLISGFIMTYVPKETILLVLSNNVYTYFYVSIIRIFIFLCPYAMLPIIKTVAIEGIPKGLIVSFLISAPTLGLPILLPMKKYFGNRLTLKYVFGVVVGSGIIGITLDKIGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDR7</accession>
    <name>F6BDR7_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="5">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">Metig_1089</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>ATP + L-seryl-[protein] = ADP + H(+) + O-phospho-L-seryl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + L-threonyl-[protein] = ADP + H(+) + O-phospho-L-threonyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the protein kinase superfamily. RIO-type Ser/Thr kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="5"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96628.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1089"/>
    <dbReference type="EnsemblBacteria" id="AEF96628">
        <property type="protein sequence ID" value="AEF96628"/>
        <property type="gene ID" value="Metig_1089"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_1089"/>
    <dbReference type="HOGENOM" id="CLU_018693_1_0_2"/>
    <dbReference type="OMA" id="GYTNFRE"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106310">
        <property type="term" value="F:protein serine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006468">
        <property type="term" value="P:protein phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05144">
        <property type="entry name" value="RIO2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030484">
        <property type="entry name" value="Rio2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015285">
        <property type="entry name" value="RIO2_wHTH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000687">
        <property type="entry name" value="RIO_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018935">
        <property type="entry name" value="RIO_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09202">
        <property type="entry name" value="Rio2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00090">
        <property type="entry name" value="RIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="SSF56112"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01245">
        <property type="entry name" value="RIO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 14" id="KW-0418">Kinase</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 14" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="7 14" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="13">
        <location>
            <begin position="65"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="262"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="AEF96628.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="284" mass="33677" checksum="A69E88981A4B84EC" modified="2011-07-27" version="1">MRYHEWVPVDEIVKKTKMDEKDVLYRLKRLNRFDFVHRSRYGYRLSFGGYDALAMNAFIKKGIIKAVGSELGVGKEGDVYNILLEDGREAVLKFHKHGRTCFTRGKRYREYLADKHHISWLYTSRLTAEREFEILNELFPIVKVPEPIEQNRHAIIMGKIYGDELKRVDLEELGVDANKLFWDIIEEIKKAYELGYIHGDLSEFNILIDENGDFVIIDWPQAVSIYHPDAEFYLKRDIGNVVRHFKRYKIKEDVDKLYEYVTNKNKNEEDENDKNDENKGEDKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDS3</accession>
    <name>F6BDS3_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Integrase family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metig_1095</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96634.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799235.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96634">
        <property type="protein sequence ID" value="AEF96634"/>
        <property type="gene ID" value="Metig_1095"/>
    </dbReference>
    <dbReference type="GeneID" id="10643953"/>
    <dbReference type="KEGG" id="mig:Metig_1095"/>
    <dbReference type="HOGENOM" id="CLU_1472098_0_0_2"/>
    <dbReference type="OrthoDB" id="100194at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.443.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011010">
        <property type="entry name" value="DNA_brk_join_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013762">
        <property type="entry name" value="Integrase-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002104">
        <property type="entry name" value="Integrase_catalytic"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00589">
        <property type="entry name" value="Phage_integrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56349">
        <property type="entry name" value="SSF56349"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51898">
        <property type="entry name" value="TYR_RECOMBINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyr recombinase" evidence="3">
        <location>
            <begin position="26"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="123"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEF96634.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="218" mass="26041" checksum="D7B25927267F62DD" modified="2011-07-27" version="1">MTWEYQLTTTNENENTKNTQYKWDLGLKYHETKDKLIKEIENLKNKEILYKNDIKRLAYLIILLTQLRNGCRIGEAITAIVGFCSNIDRINWEYPIIAVKVEKRKDNHFREIILPKYIKKHDLEIIKNFILNLKDEYEKEENKEKAHKKIISKISMWAKRNLGINTHSLRYAYITYKAEKQRPAQIIAKITGHKNLDMIVDYTQTQLARKELLNEEDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2020-08-12" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDT6</accession>
    <name>F6BDT6_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_1108</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96647.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96647">
        <property type="protein sequence ID" value="AEF96647"/>
        <property type="gene ID" value="Metig_1108"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_1108"/>
    <dbReference type="HOGENOM" id="CLU_1801702_0_0_2"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF96647.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="143" mass="17056" checksum="AE1DC8BB69BC0387" modified="2011-07-27" version="1">MEDRDALNLINLKILRLDEEIMMDFDSEVRSISKIALKVCGYEIKNEIELKLKGRRRRKLSEKQINRINKLINMLELIKEFIERKEFKSSIDYEALFLISLKVHRIEEGILTNFEEEVDSILKMARKVGNYELRKRIEEILGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2020-08-12" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDU3</accession>
    <name>F6BDU3_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_1115</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96654.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96654">
        <property type="protein sequence ID" value="AEF96654"/>
        <property type="gene ID" value="Metig_1115"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_1115"/>
    <dbReference type="HOGENOM" id="CLU_1048128_0_0_2"/>
    <dbReference type="OMA" id="VYIDVFM"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF96654.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="269" mass="32339" checksum="0280FB189FD717D4" modified="2011-07-27" version="1">MVNVMHAKEMASKVLQDIYKNLDEYCKDLIRTDLVDVEFKGFYMRTLDKEKIFIRDLEDYEHLPDVEVENRKYKLKRVNLKHVDDGLMIIHLSSRKAKEYEFEVDSAYEVVYPTSDVTYEHRERILKWMELDDEDLDKLISEFDARVEDLLSGILVNSKIKKEVLVYVDVFMDLEKIKNYIESDEERIILWLHPMYLFSGDEVLKGIITYELSRYDKNLLEIYYKDVIEYCKEYKKLCNKNLKVLEKIKNIAIERNDTKSIEIIKEIQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-09-29" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDV3</accession>
    <name>F6BDV3_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1125</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96664.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799265.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96664">
        <property type="protein sequence ID" value="AEF96664"/>
        <property type="gene ID" value="Metig_1125"/>
    </dbReference>
    <dbReference type="GeneID" id="10643983"/>
    <dbReference type="KEGG" id="mig:Metig_1125"/>
    <dbReference type="HOGENOM" id="CLU_1140581_0_0_2"/>
    <dbReference type="OMA" id="WKGHTIL"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007404">
        <property type="entry name" value="YdjM-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04307">
        <property type="entry name" value="YdjM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="48"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="72"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="103"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="138"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="168"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="206"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96664.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="231" mass="26204" checksum="9CB0E6D7CD215E91" modified="2011-07-27" version="1">MDWKGHTILGLVFGLPFISSPEQIFLVLAGALYPDLDHDVKEDIVKRGIYISLGLVLINILIYYFKKEYFNLDLFILAVSVLLIYLIPYFANHRGITHTFWALIFVSVILGFLALKLTIFSPILAGIIVLLMVVNEKLLGKILIIALFGWIVFDILKPNPLVSGNFSYILPIAVGYLSHIVGDSTTPAGVKAFYPISNYKLRKKEGYVLILVWFLMVLYILERCNIVFINF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDV7</accession>
    <name>F6BDV7_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Radical SAM domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Metig_1129</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>2Fe-2S cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49601"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96668.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799269.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1129"/>
    <dbReference type="EnsemblBacteria" id="AEF96668">
        <property type="protein sequence ID" value="AEF96668"/>
        <property type="gene ID" value="Metig_1129"/>
    </dbReference>
    <dbReference type="GeneID" id="10643987"/>
    <dbReference type="KEGG" id="mig:Metig_1129"/>
    <dbReference type="HOGENOM" id="CLU_033172_0_0_2"/>
    <dbReference type="OMA" id="IMGFNPY"/>
    <dbReference type="OrthoDB" id="35292at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004076">
        <property type="term" value="F:biotin synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009102">
        <property type="term" value="P:biotin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010722">
        <property type="entry name" value="BATS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002684">
        <property type="entry name" value="Biotin_synth/BioAB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaB/NifB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023858">
        <property type="entry name" value="RSAM_HmdB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22976">
        <property type="entry name" value="PTHR22976"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06968">
        <property type="entry name" value="BATS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00330">
        <property type="entry name" value="HMD_cofactor_maturase_(HmdB-li"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00876">
        <property type="entry name" value="BATS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03957">
        <property type="entry name" value="rSAM_HmdB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="7" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="8">
        <location>
            <begin position="59"/>
            <end position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AEF96668.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="350" mass="39385" checksum="A7D8BF2D72314AAF" modified="2011-07-27" version="1">MVFKKIEENFEELKKGNEEFIKYGLIDKEDALKLFEINHWSDYLRLFNIASKVRDYFKKEIEITSTIHITNICKVNPKCHYCGFAAGTSREGYYKPFRISDEDIKKSAIAIEESGICRVSCSSAHGYGGREVLRALKIVKENTNLEVLVNAGADLTEETIKEMKKYGIDTICCNLETTNEELFNKVKPGEKLEDRIKVCKLVRKYDIELSSGLLIGIGESYEDRVEHLFYLKELGVGEIPIMGFNPYKDTPMENHPKCSALEQAKTIAITRLIFPDIRITSPTPTIGAELVQFALLGGASNLATVIPDNHPMNIKGVGNPRTGNLNEVIKMISELGLKPKLNLSKINSKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDW7</accession>
    <name>F6BDW7_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="3">ATP-grasp fold domain protein, DUF201-type</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_1139</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96678.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799279.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1139"/>
    <dbReference type="EnsemblBacteria" id="AEF96678">
        <property type="protein sequence ID" value="AEF96678"/>
        <property type="gene ID" value="Metig_1139"/>
    </dbReference>
    <dbReference type="GeneID" id="10643997"/>
    <dbReference type="KEGG" id="mig:Metig_1139"/>
    <dbReference type="HOGENOM" id="CLU_057102_0_0_2"/>
    <dbReference type="OMA" id="FLCFNKQ"/>
    <dbReference type="OrthoDB" id="44157at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003806">
        <property type="entry name" value="ATP-grasp_PylC-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005479">
        <property type="entry name" value="CbamoylP_synth_lsu-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016677">
        <property type="entry name" value="UCP016817_carboligase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02655">
        <property type="entry name" value="ATP-grasp_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016817">
        <property type="entry name" value="UCP016817_carboligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00867">
        <property type="entry name" value="CPSASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-grasp" evidence="2">
        <location>
            <begin position="112"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF96678.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="368" mass="42106" checksum="41432F5F65434E30" modified="2011-07-27" version="1">MRLLVVGVNTRPVVNSAKRLGFEVYSVSYYNPVDLNADKKIYFIDDYNHGHFRENYSEDELLKAAEKFVDEVDGILITSGIFEDKNSKTPNWDVIGNPPKKIKRMSNKYNTIKRLENLGYNVPLTYLAKNYSQLERYLDELKIAILKPISGSGGIGVTKISFSTIENMGAKEALEELNINIEFPILVQEYINSESFSASFIDTNFITFNRQIIENNMYVGNITPYSLNGRIFNVSKAINDFREIIETFELKGMNGIDFMIKDNEVCIIEINPRILGTFETIEKSAGVNLVKHIIEGKSANVRERFIKRIVFAKEKIIAKIKKKPFVYDIPKYNAVIEKNEPIATVIAKTNINHCIDSIYKECVEYEAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-06-02" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDZ8</accession>
    <name>F6BDZ8_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Citrate transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Metig_1171</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96709.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799309.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96709">
        <property type="protein sequence ID" value="AEF96709"/>
        <property type="gene ID" value="Metig_1171"/>
    </dbReference>
    <dbReference type="GeneID" id="10644031"/>
    <dbReference type="KEGG" id="mig:Metig_1171"/>
    <dbReference type="HOGENOM" id="CLU_063025_0_0_2"/>
    <dbReference type="OMA" id="ICSMFVT"/>
    <dbReference type="OrthoDB" id="49249at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004680">
        <property type="entry name" value="Cit_transptr-like_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03600">
        <property type="entry name" value="CitMHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="9"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="35"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="74"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="151"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="191"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="229"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="268"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="296"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="330"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="domain" description="CitMHS" evidence="7">
        <location>
            <begin position="20"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF03600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEF96709.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="357" mass="41285" checksum="1D8C3F4EE6A5D361" modified="2011-07-27" version="1">MVRINIDETIFLFFIFISILFLIFLIKPIEILDIVEWKTIFSLFYMLVVVNLLKDCGFLEWISLKIIEKTNRIFIVLILLTLLLSMFVTNDIALFVIIPITLIMTKYCNINLRKILMLEGISANIGSALTPFGNPQNLFIYYHYNLNLMEFIINMIPFEILGILILIPFLEFKKMNVDVNTVKTQKFKKEWLSYLAIFLLVIASILGIFNYIYLLPVILPIAFIKRIKIDYLFILTFCALFIDIEGLKRLGVIELFKISYENDILTMIYASLLSQIISNVPTTILLSNIYNNWLPIAYGVNVGGNGTLIASFANLITLRLSDGKISAIKFLMFGILVYILHLIALIIYFYVKNRFLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>F6BE03</accession>
    <name>F6BE03_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Sodium/hydrogen exchanger</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Metig_1176</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96714.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1176"/>
    <dbReference type="EnsemblBacteria" id="AEF96714">
        <property type="protein sequence ID" value="AEF96714"/>
        <property type="gene ID" value="Metig_1176"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_1176"/>
    <dbReference type="HOGENOM" id="CLU_005126_7_1_2"/>
    <dbReference type="OMA" id="EDKGDMQ"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015299">
        <property type="term" value="F:solute:proton antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006812">
        <property type="term" value="P:cation transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006153">
        <property type="entry name" value="Cation/H_exchanger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038770">
        <property type="entry name" value="Na+/solute_symporter_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00999">
        <property type="entry name" value="Na_H_Exchanger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="20"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="45"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="70"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="101"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="131"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="161"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="194"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="227"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="280"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="313"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="343"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="374"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="domain" description="Na_H_Exchanger" evidence="5">
        <location>
            <begin position="25"/>
            <end position="398"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEF96714.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="408" mass="44595" checksum="5D249B653D90D8F7" modified="2011-07-27" version="1">MSKYMQKTVYTMVHGEIVDEYLLFFIIIATVFIVPNLLRKFNVPAITSIMLAGILIGPHGLNILQVDGTLKIFSSFGAIFLMFLAGLEVDNETLREEFKNSMVVSLFSLLIPGVGGYLVGQYLGLGFAGSLLYSVIFASHSVGIVYALMDELKMVKTKLGTMMLSATILIDLITLILLSIVIKLSETNAITSDIWMFLIYAGLYIGGLLLTIPYLSKIIFKNLEKLHIQRIHFVLFIILISILIGEHLGLHPIVGAFITGVAVSEALTKEEYDELLNKNLNAIGYGFFIPIFFLVLGMETNVGVIFNLNNLELVLITIVSAVLLKLTSGLISLRILGYDKLKSFVGSLLTVPKISASLVAASVGRELGIISDDVFVAIVALSIVTAMITPIGVKSIVVKHLDRFLGDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>F6BE14</accession>
    <name>F6BE14_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">4Fe-4S ferredoxin iron-sulfur binding domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1187</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96725.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799324.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1187"/>
    <dbReference type="EnsemblBacteria" id="AEF96725">
        <property type="protein sequence ID" value="AEF96725"/>
        <property type="gene ID" value="Metig_1187"/>
    </dbReference>
    <dbReference type="GeneID" id="10644047"/>
    <dbReference type="KEGG" id="mig:Metig_1187"/>
    <dbReference type="HOGENOM" id="CLU_139698_11_4_2"/>
    <dbReference type="OMA" id="AGCVPFC"/>
    <dbReference type="OrthoDB" id="111853at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00037">
        <property type="entry name" value="Fer4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="26"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96725.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="54" mass="6006" checksum="AA669F75B372C53C" modified="2011-07-27" version="1">MRILDRCVGCGECVPFCPFEAIKTYGKAIIDKEKCTNCGICIKYCPISAIEEDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>F6BE19</accession>
    <name>F6BE19_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Molybdopterin oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Metig_1192</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96730.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799329.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1192"/>
    <dbReference type="EnsemblBacteria" id="AEF96730">
        <property type="protein sequence ID" value="AEF96730"/>
        <property type="gene ID" value="Metig_1192"/>
    </dbReference>
    <dbReference type="GeneID" id="10644052"/>
    <dbReference type="KEGG" id="mig:Metig_1192"/>
    <dbReference type="HOGENOM" id="CLU_039904_0_0_2"/>
    <dbReference type="OMA" id="GHCICNS"/>
    <dbReference type="OrthoDB" id="1029at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006656">
        <property type="entry name" value="Mopterin_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006963">
        <property type="entry name" value="Mopterin_OxRdtase_4Fe-4S_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006655">
        <property type="entry name" value="Mopterin_OxRdtase_prok_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027467">
        <property type="entry name" value="MopterinOxRdtase_cofactor_BS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04879">
        <property type="entry name" value="Molybdop_Fe4S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00384">
        <property type="entry name" value="Molybdopterin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00926">
        <property type="entry name" value="Molybdop_Fe4S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51669">
        <property type="entry name" value="4FE4S_MOW_BIS_MGD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00551">
        <property type="entry name" value="MOLYBDOPTERIN_PROK_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00490">
        <property type="entry name" value="MOLYBDOPTERIN_PROK_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="2 4" id="KW-0408">Iron</keyword>
    <keyword evidence="3 4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S Mo/W bis-MGD-type" evidence="5">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="8"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="11"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="15"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="43"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEF96730.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="398" mass="44418" checksum="0A680211C228EB11" modified="2011-07-27" version="1">MKIIHTICPSCSVGCGIDLIVENDKVVGTYPYKKHTINEGKNCLTGKNCYKIIYHEKRLKKPLIKKNGKFVEVSWDEALELIANKLKSYNPEDIGFIASGKCTNEDNYALKKFAETLGVKNIGHCICNSPKVGLDKETASIDDIENAEVILIFGDVFGQNALIGRKVIKAKEKGADVIVIDRIEKEITKLNSDEFIKVNDFVEFLSNPNDEIVKKLSEKNSIIIFNALTTEEECDLAYKLAEKTNSKILPIAKHCNTVGATMVGISALNKDEIINLIKSVKCLYIMGENPALIDDEALKNVEFLVVQDIIMSETAELADVVLPSACWAEKEGTFTNTERRTQKINKAVNPPGEALEDWRIIKNLAEKMGIDLGFSSVEDIQKNYVKFKQVSYSRSTIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F6BE54</accession>
    <name>F6BE54_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Methyltransferase type 11</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0029</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95590.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798200.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0029"/>
    <dbReference type="EnsemblBacteria" id="AEF95590">
        <property type="protein sequence ID" value="AEF95590"/>
        <property type="gene ID" value="Metig_0029"/>
    </dbReference>
    <dbReference type="GeneID" id="10642864"/>
    <dbReference type="KEGG" id="mig:Metig_0029"/>
    <dbReference type="HOGENOM" id="CLU_037990_14_1_2"/>
    <dbReference type="OMA" id="CIVPRES"/>
    <dbReference type="OrthoDB" id="45753at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013216">
        <property type="entry name" value="Methyltransf_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08241">
        <property type="entry name" value="Methyltransf_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransf_11" evidence="1">
        <location>
            <begin position="47"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF95590.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="200" mass="23150" checksum="C4BAF8A6C013E49D" modified="2011-07-27" version="1">MKNNHNPFDKYAEDYDKWYDENEEIYKLELEAIEKFIPKPKEKKKGLEIGVGTGRFAKPLGIKYGLEPSKSMAEIAKRRGIEVYEGIAENIPFDDGSFDYVLMTTTLCFLNDPIKGLNEIKRILKPNGVVIIGMIDKNSPLGIFYESKKEKSKFYKNAKFYSLNEVLNWLKELEFKNIIYNPVKLDKNDTGSFVVIRAEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>F6BE61</accession>
    <name>F6BE61_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Coenzyme F420 hydrogenase, subunit alpha</fullName>
            <ecNumber evidence="3">1.12.98.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_1200</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit family.</text>
    </comment>
    <dbReference type="EC" id="1.12.98.1" evidence="3"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96738.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799337.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1200"/>
    <dbReference type="EnsemblBacteria" id="AEF96738">
        <property type="protein sequence ID" value="AEF96738"/>
        <property type="gene ID" value="Metig_1200"/>
    </dbReference>
    <dbReference type="GeneID" id="10644060"/>
    <dbReference type="KEGG" id="mig:Metig_1200"/>
    <dbReference type="HOGENOM" id="CLU_044556_1_0_2"/>
    <dbReference type="OMA" id="FMPDFTR"/>
    <dbReference type="OrthoDB" id="32760at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050454">
        <property type="term" value="F:coenzyme F420 hydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008901">
        <property type="term" value="F:ferredoxin hydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016151">
        <property type="term" value="F:nickel cation binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.645.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017682">
        <property type="entry name" value="Coenz_F420_hydrogenase_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001501">
        <property type="entry name" value="Ni-dep_hyd_lsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018194">
        <property type="entry name" value="Ni-dep_hyd_lsu_Ni_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029014">
        <property type="entry name" value="NiFe-Hase_large"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00374">
        <property type="entry name" value="NiFeSe_Hases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56762">
        <property type="entry name" value="SSF56762"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03295">
        <property type="entry name" value="frhA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00507">
        <property type="entry name" value="NI_HGENASE_L_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00508">
        <property type="entry name" value="NI_HGENASE_L_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0533">Nickel</keyword>
    <keyword evidence="1 2 3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF96738.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="410" mass="45984" checksum="9E579550FA95E0AA" modified="2011-07-27" version="1">MGKVVEIHPTTRHEGHAKLVLKVDDEGIVEKGYYLSVTPVRGFEKFLVGKPAEFAPIAVMRFCGVCPVAHAISSVEAIEDACNITPPKDGLLLRELIGIANKMHSHPLHQFLISPDFVPEKDRVEFIKRVQKMRKIAQYIVDVVGGEGIHPPNIRIGGMAKNITESAKTKIYYKCKEYEKLAKEQIEYLVPIFESRTLTNGTELPEKLGYHDFGYIATHPTYGDRTAIDQSKVVEYTPFEFYEKDVALQASTTIPTYNGRLAEVGPRARFRKFFDFKEKGAMAINIARAYELEVLVKRAMEILDELNVNGKTLSDEPIIGDGEKAGLGVHEAARGHNTHQAAIDKRGNIVYYNAIVATTWNIPVISKAIEGSHYKFAEYVVRAYDPCISCATHMIVKDYDGRVVDEKILK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>F6BE69</accession>
    <name>F6BE69_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="4">CoA-substrate-specific enzyme activase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metig_1208</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96746.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799345.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1208"/>
    <dbReference type="EnsemblBacteria" id="AEF96746">
        <property type="protein sequence ID" value="AEF96746"/>
        <property type="gene ID" value="Metig_1208"/>
    </dbReference>
    <dbReference type="GeneID" id="10644068"/>
    <dbReference type="KEGG" id="mig:Metig_1208"/>
    <dbReference type="HOGENOM" id="CLU_066597_0_0_2"/>
    <dbReference type="OMA" id="GKGANYF"/>
    <dbReference type="OrthoDB" id="72761at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002731">
        <property type="entry name" value="ATPase_BadF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008275">
        <property type="entry name" value="CoA_E_activase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01869">
        <property type="entry name" value="BcrAD_BadFG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="SSF53067"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00241">
        <property type="entry name" value="CoA_E_activ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0408">Iron</keyword>
    <keyword evidence="2" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BcrAD_BadFG" evidence="3">
        <location>
            <begin position="3"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEF96746.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="244" mass="26841" checksum="FE1C5C098C4AB104" modified="2011-07-27" version="1">MILGIDVGSTTTKMVLMENNKIVDYKIKNIGVVVEEEDILKMVNEFEKEYSVDKVVATGYGRHKITFADRVVPEVIALGRGANYFFKEADGVVDIGGQDSKVIKIDKDGNVVDFILSDKCAAGTGKFLEKCIEILKIEDDINKYKSNNIAKISSMCAVFAESEIISLLAKKTPKENILMGVYESICNRIIPMVNKLGINNIVFSGGVAKNRVLAEVLEEKLNKSILIPKEPQIVCCVGAILKGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>F6BE81</accession>
    <name>F6BE81_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">50S ribosomal protein L4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">rpl4</name>
        <name evidence="6" type="ordered locus">Metig_1221</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Forms part of the polypeptide exit tunnel.</text>
    </comment>
    <comment type="function">
        <text evidence="4">One of the primary rRNA binding proteins, this protein initially binds near the 5'-end of the 23S rRNA. It is important during the early stages of 50S assembly. It makes multiple contacts with different domains of the 23S rRNA in the assembled 50S subunit and ribosome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the universal ribosomal protein uL4 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96758.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799357.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1221"/>
    <dbReference type="EnsemblBacteria" id="AEF96758">
        <property type="protein sequence ID" value="AEF96758"/>
        <property type="gene ID" value="Metig_1221"/>
    </dbReference>
    <dbReference type="GeneID" id="10644082"/>
    <dbReference type="KEGG" id="mig:Metig_1221"/>
    <dbReference type="HOGENOM" id="CLU_026535_0_0_2"/>
    <dbReference type="OMA" id="WHQKVNV"/>
    <dbReference type="OrthoDB" id="65833at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1370.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01328_A">
        <property type="entry name" value="Ribosomal_L4_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002136">
        <property type="entry name" value="Ribosomal_L4/L1e"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013000">
        <property type="entry name" value="Ribosomal_L4/L1e_euk/arc_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023574">
        <property type="entry name" value="Ribosomal_L4_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045240">
        <property type="entry name" value="Ribosomal_L4_euk/arch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019970">
        <property type="entry name" value="Ribosomall_L4-archaea"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19431">
        <property type="entry name" value="PTHR19431"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00573">
        <property type="entry name" value="Ribosomal_L4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52166">
        <property type="entry name" value="SSF52166"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03672">
        <property type="entry name" value="rpl4p_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00939">
        <property type="entry name" value="RIBOSOMAL_L1E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 6" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="4" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0699">rRNA-binding</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="46"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEF96758.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="254" mass="27941" checksum="0A315AA0836C9615" modified="2011-07-27" version="1">MKAVVYNLDGSKKGEIELPSIFETEYRPDLIKRAVISALTARLQPKGSDPLAGKRTSAESIGKGHGRARVKRTAQGWAAFVPQAVGGRRCHPPKVEKILWERINKKERLKAIMSAIAASANPELVKQRGHIIDNVPSIPLVVDNEFENLQKTKDVVNVFKALGLDKDVERAKKGIKIRAGRGKMRGRKYRKPKSVLVVVGDKCNAIKASRNLPGVDVITADNFGVIHIAPGAMAGRLTLWTESAIEKLSERFKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEC9</accession>
    <name>F6BEC9_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">6-carboxyhexanoate--CoA ligase</fullName>
            <ecNumber evidence="8">6.2.1.14</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Pimeloyl-CoA synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">bioW</name>
        <name evidence="9" type="ordered locus">Metig_1269</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the transformation of pimelate into pimeloyl-CoA with concomitant hydrolysis of ATP to AMP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>ATP + CoA + heptanedioate = 6-carboxyhexanoyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:14781"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:36165"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57360"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.2.1.14"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 8">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="8">Metabolic intermediate metabolism; pimeloyl-CoA biosynthesis; pimeloyl-CoA from pimelate: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 8">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the BioW family.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.14" evidence="8"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96806.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799404.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1269"/>
    <dbReference type="EnsemblBacteria" id="AEF96806">
        <property type="protein sequence ID" value="AEF96806"/>
        <property type="gene ID" value="Metig_1269"/>
    </dbReference>
    <dbReference type="GeneID" id="10644132"/>
    <dbReference type="KEGG" id="mig:Metig_1269"/>
    <dbReference type="HOGENOM" id="CLU_076858_0_0_2"/>
    <dbReference type="OMA" id="LCWSDDP"/>
    <dbReference type="OrthoDB" id="68002at2157"/>
    <dbReference type="UniPathway" id="UPA00999">
        <property type="reaction ID" value="UER00351"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042410">
        <property type="term" value="F:6-carboxyhexanoate-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009102">
        <property type="term" value="P:biotin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00668">
        <property type="entry name" value="BioW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005499">
        <property type="entry name" value="BioW"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03744">
        <property type="entry name" value="BioW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01204">
        <property type="entry name" value="bioW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 8" id="KW-0093">Biotin biosynthesis</keyword>
    <keyword evidence="3 8 9" id="KW-0436">Ligase</keyword>
    <keyword evidence="7 8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AEF96806.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="233" mass="26598" checksum="E4E46971C630F21E" modified="2011-07-27" version="1">MYSIKMRASKNGKHISGAERIVNKEEIELVANELIKRALMHENGTPDFINLKIEEIKEEIEYIDHLPIKTIECKNKREAREKARELLKNEGISDELIDYAFKIIDKGGMRGAAILNLRGERLEPDKERGVRVKNIDTTKELKEKILKEKLGTERTVDAIAIASKVIHLGIIAELCTSDNKSYTTGYVATKKGYFRITNLKEKGENGGRVFFVKNDVDIEDLINKLENKPFIIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-06-02" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEG4</accession>
    <name>F6BEG4_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">UbiA prenyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0064</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95625.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798234.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF95625">
        <property type="protein sequence ID" value="AEF95625"/>
        <property type="gene ID" value="Metig_0064"/>
    </dbReference>
    <dbReference type="GeneID" id="10642899"/>
    <dbReference type="KEGG" id="mig:Metig_0064"/>
    <dbReference type="HOGENOM" id="CLU_3057174_0_0_2"/>
    <dbReference type="OMA" id="NILIVAM"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="30"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF95625.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="54" mass="6115" checksum="B7E22E06B814E00F" modified="2011-07-27" version="1">MKKETKIKILIFINILIVAMTLWALLRGKILLTLVGIVVLINGLLTLKRAKEEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEH0</accession>
    <name>F6BEH0_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">ABC-type nitrate/sulfonate/bicarbonate transport system, periplasmic component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_0070</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95631.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0070"/>
    <dbReference type="EnsemblBacteria" id="AEF95631">
        <property type="protein sequence ID" value="AEF95631"/>
        <property type="gene ID" value="Metig_0070"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0070"/>
    <dbReference type="HOGENOM" id="CLU_028871_10_4_2"/>
    <dbReference type="OMA" id="PEGMWEN"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF95631.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="404" mass="45191" checksum="4BA929DD7D22295B" modified="2011-07-27" version="1">MQKNQKFYIVVFYLTHVVKGGDTIKKYVILLSSILIAGVLFSGCMQNETKTETPTLKVAYLPTDHHAALFVAAKEGNLFKDKYGIYMKEIEPKKKYELYENGKKVADVELVQVVEGGAKIMTLMAQNQIDIGLNGVPPAVFAIDKGTKAKIISALQGEGSAVVIRKDIPANNWEEFINWIKEQHANGKQVKIGYPLPISIQYVMIKKALEAEGITYTEDPNNKEAMVLLINCKGQKSMPQMLSQKELDAVIAWEPTPEVLKTEGIGKPIVYSKDLPPKGMWKDHPCCVIAASEDALNNKRDAVKAFLKLIVLATKEINENKELVIKDSAEWLGVDEKVEEESIPNMKFDTNPEPLKEGTYLFVDVMNKQGAMDGKLKGVSNKSEIDNILFDFSIYNEIMKELNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEH7</accession>
    <name>F6BEH7_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Precorrin-6y C5,15-methyltransferase (Decarboxylating), CbiE subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Metig_0077</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; adenosylcobalamin biosynthesis.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95638.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798247.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0077"/>
    <dbReference type="EnsemblBacteria" id="AEF95638">
        <property type="protein sequence ID" value="AEF95638"/>
        <property type="gene ID" value="Metig_0077"/>
    </dbReference>
    <dbReference type="GeneID" id="10642912"/>
    <dbReference type="KEGG" id="mig:Metig_0077"/>
    <dbReference type="HOGENOM" id="CLU_089162_2_0_2"/>
    <dbReference type="OMA" id="DPCFSGL"/>
    <dbReference type="OrthoDB" id="90646at2157"/>
    <dbReference type="UniPathway" id="UPA00148"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008276">
        <property type="term" value="F:protein methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd11644">
        <property type="entry name" value="Precorrin-6Y-MT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.950.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1010.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000878">
        <property type="entry name" value="4pyrrol_Mease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035996">
        <property type="entry name" value="4pyrrol_Methylase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014777">
        <property type="entry name" value="4pyrrole_Mease_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014776">
        <property type="entry name" value="4pyrrole_Mease_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012818">
        <property type="entry name" value="CbiE"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00590">
        <property type="entry name" value="TP_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53790">
        <property type="entry name" value="SSF53790"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02467">
        <property type="entry name" value="CbiE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 6" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="TP_methylase" evidence="5">
        <location>
            <begin position="1"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEF95638.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="209" mass="23831" checksum="F6E017E5D43CA5E3" modified="2011-07-27" version="1">MIYIVGIGPGDKRYLTLMAIEIVKNSDMVVGSKRALELFDIEEDKKCYLTKNLREELKEIINSTKNKNINIAILSTGDPCFSGLLKTILSLGVRKEDIEVISGISSIQIAAAKLKISWEDYEIITLHGKEENRKKLLNLIKNNQKVIFLPNNLKEDIEYLINNGVNPEKEMTVCENLTYENERIVRDSLKNLLKMDFSYLCVCVLEGDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEI7</accession>
    <name>F6BEI7_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 11">Methenyltetrahydromethanopterin cyclohydrolase</fullName>
            <ecNumber evidence="4 11">3.5.4.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10 11">Methenyl-H4MPT cyclohydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">mch</name>
        <name evidence="12" type="ordered locus">Metig_0087</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes the reversible interconversion of 5-formyl-H(4)MPT to methenyl-H(4)MPT(+).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 11">
            <text>5,10-methenyl-5,6,7,8-tetrahydromethanopterin + H2O = H(+) + N(5)-formyl-5,6,7,8-tetrahydromethanopterin</text>
            <dbReference type="Rhea" id="RHEA:19053"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58018"/>
            <dbReference type="ChEBI" id="CHEBI:58337"/>
            <dbReference type="EC" id="3.5.4.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="11">One-carbon metabolism; methanogenesis from CO(2); 5,10-methenyl-5,6,7,8-tetrahydromethanopterin from CO(2): step 3/3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 11">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the MCH family.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.27" evidence="4 11"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95648.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798257.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0087"/>
    <dbReference type="EnsemblBacteria" id="AEF95648">
        <property type="protein sequence ID" value="AEF95648"/>
        <property type="gene ID" value="Metig_0087"/>
    </dbReference>
    <dbReference type="GeneID" id="10642922"/>
    <dbReference type="KEGG" id="mig:Metig_0087"/>
    <dbReference type="HOGENOM" id="CLU_876031_0_0_2"/>
    <dbReference type="OMA" id="DKGMFAP"/>
    <dbReference type="OrthoDB" id="31274at2157"/>
    <dbReference type="UniPathway" id="UPA00640">
        <property type="reaction ID" value="UER00694"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018759">
        <property type="term" value="F:methenyltetrahydromethanopterin cyclohydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019386">
        <property type="term" value="P:methanogenesis, from carbon dioxide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00545">
        <property type="entry name" value="MCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00486">
        <property type="entry name" value="McH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003209">
        <property type="entry name" value="METHMP_CycHdrlase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02289">
        <property type="entry name" value="MCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03120">
        <property type="entry name" value="one_C_mch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 11 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9 11" id="KW-0484">Methanogenesis</keyword>
    <keyword evidence="7 11" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00020597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00030468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AEF95648.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="326" mass="35281" checksum="7793434AD948C9A2" modified="2011-07-27" version="1">MLSVNLKAFEIVKKMMENAEELNIAVKKLENGATVLDCGVNVPGSWEAGKLFTKICLGGLAHVGISLSPCECNGLNLPHVKVKTSHPAIATLGAQKAGWAIKVGKYFAMGSGPARALAKKPKETYEEIGYEDDADIAVLCLEASALPNEEVAEYVANQCNVEPSNVYLLVAPTASLVGSIQISGRVVENGTYKMLEVLDFDVKKVKFAAGIAPIAPIIGDDFAMMGATNDMVLYGGRTFYYIESDENDDIEALCKALPSCTSADYGKPFMEVFKAANYDFYKIDKGMFAPAVVTINDMRTGKVYTYGEINVEVLKKSLKIVELKKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-06-02" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEM2</accession>
    <name>F6BEM2_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_1282</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96819.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799416.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96819">
        <property type="protein sequence ID" value="AEF96819"/>
        <property type="gene ID" value="Metig_1282"/>
    </dbReference>
    <dbReference type="GeneID" id="10644145"/>
    <dbReference type="KEGG" id="mig:Metig_1282"/>
    <dbReference type="HOGENOM" id="CLU_2662346_0_0_2"/>
    <dbReference type="OrthoDB" id="144064at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF96819.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="75" mass="8721" checksum="37CC4775918EAE35" modified="2011-07-27" version="1">MIICLALQDVPNYKADILLNNNEEIKDVYIIEDNPEGYILVLDENDRITKIMKSSIIKIEYKKHDLVKNENSDVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2020-08-12" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEQ0</accession>
    <name>F6BEQ0_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_1310</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96847.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96847">
        <property type="protein sequence ID" value="AEF96847"/>
        <property type="gene ID" value="Metig_1310"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_1310"/>
    <dbReference type="HOGENOM" id="CLU_2802250_0_0_2"/>
    <dbReference type="OMA" id="DKTLIFM"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF96847.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="67" mass="7844" checksum="1219B8C00D254A37" modified="2011-07-27" version="1">MAKKSATLHVKVYYPGVVLERDFDFDVVAKLKEKYLRYLDTGRANGAILYNHEQKKFAIIDLKKFVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>F6BER1</accession>
    <name>F6BER1_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Chorismate mutase, type II</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_1321</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96858.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799455.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1321"/>
    <dbReference type="EnsemblBacteria" id="AEF96858">
        <property type="protein sequence ID" value="AEF96858"/>
        <property type="gene ID" value="Metig_1321"/>
    </dbReference>
    <dbReference type="GeneID" id="10644184"/>
    <dbReference type="KEGG" id="mig:Metig_1321"/>
    <dbReference type="HOGENOM" id="CLU_131518_4_0_2"/>
    <dbReference type="OMA" id="MDYNKEV"/>
    <dbReference type="OrthoDB" id="110308at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004106">
        <property type="term" value="F:chorismate mutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046417">
        <property type="term" value="P:chorismate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.59.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036263">
        <property type="entry name" value="Chorismate_II_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036979">
        <property type="entry name" value="CM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002701">
        <property type="entry name" value="CM_II_prokaryot"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01817">
        <property type="entry name" value="CM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00830">
        <property type="entry name" value="CM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48600">
        <property type="entry name" value="SSF48600"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51168">
        <property type="entry name" value="CHORISMATE_MUT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Chorismate mutase" evidence="2">
        <location>
            <begin position="2"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF96858.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="95" mass="11269" checksum="18061BF9D7D6100F" modified="2011-07-27" version="1">MNDAKTQLEAIRKRIDEIDEELIKLMAERTNFAKDIAMLKKALNMPINDSKREKEVYEKTRKLCNKYNFDEEIGVKIIKILVEHNKAIQSKYLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>F6BES3</accession>
    <name>F6BES3_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 13">Archaeal Lon protease</fullName>
            <ecNumber evidence="13">3.4.21.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">ATP-dependent protease La homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ordered locus">Metig_1333</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">ATP-dependent serine protease that mediates the selective degradation of mutant and abnormal proteins as well as certain short-lived regulatory proteins. Degrades polypeptides processively.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11 13">Homohexamer. Organized in a ring with a central cavity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 13">Cell membrane</location>
            <topology evidence="2 13">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the peptidase S16 family. Archaeal LonB subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.-" evidence="13"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96870.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799467.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1333"/>
    <dbReference type="MEROPS" id="S16.008"/>
    <dbReference type="EnsemblBacteria" id="AEF96870">
        <property type="protein sequence ID" value="AEF96870"/>
        <property type="gene ID" value="Metig_1333"/>
    </dbReference>
    <dbReference type="GeneID" id="10644196"/>
    <dbReference type="KEGG" id="mig:Metig_1333"/>
    <dbReference type="HOGENOM" id="CLU_392630_0_0_2"/>
    <dbReference type="OMA" id="IPCSHIS"/>
    <dbReference type="OrthoDB" id="7657at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004176">
        <property type="term" value="F:ATP-dependent peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030163">
        <property type="term" value="P:protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041699">
        <property type="entry name" value="AAA_32"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004663">
        <property type="entry name" value="Lon_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008269">
        <property type="entry name" value="Lon_proteolytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027065">
        <property type="entry name" value="Lon_Prtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000523">
        <property type="entry name" value="Mg_chelatse_chII-like_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025943">
        <property type="entry name" value="Sigma_54_int_dom_ATP-bd_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10046">
        <property type="entry name" value="PTHR10046"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13654">
        <property type="entry name" value="AAA_32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05362">
        <property type="entry name" value="Lon_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01078">
        <property type="entry name" value="Mg_chelatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00764">
        <property type="entry name" value="lon_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51786">
        <property type="entry name" value="LON_PROTEOLYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00676">
        <property type="entry name" value="SIGMA54_INTERACT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 13" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 12 13" id="KW-0645">Protease</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 12 13" id="KW-0720">Serine protease</keyword>
    <keyword evidence="7 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="116"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="143"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="domain" description="Lon proteolytic" evidence="14">
        <location>
            <begin position="456"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="active site" evidence="12">
        <location>
            <position position="550"/>
        </location>
    </feature>
    <feature type="active site" evidence="12">
        <location>
            <position position="593"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009579"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00026070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU369001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="AEF96870.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="645" mass="70957" checksum="43FDDE60B4E1A6AC" modified="2011-07-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEU1</accession>
    <name>F6BEU1_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="3">CBS domain containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_0116</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95677.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0116"/>
    <dbReference type="EnsemblBacteria" id="AEF95677">
        <property type="protein sequence ID" value="AEF95677"/>
        <property type="gene ID" value="Metig_0116"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0116"/>
    <dbReference type="HOGENOM" id="CLU_2044437_0_0_2"/>
    <dbReference type="OMA" id="CKHSKIE"/>
    <dbReference type="OrthoDB" id="104239at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="SSF54631"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0129">CBS domain</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBS" evidence="2">
        <location>
            <begin position="10"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="2">
        <location>
            <begin position="68"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF95677.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="123" mass="14225" checksum="D695C9FA8BD133D7" modified="2011-07-27" version="1">MIIMKIKEVMNKEFIELSPNDIGGEVVQMLYKKRKNYAPVVEDGKLVGWITTLDLLAGCKHSKIEDLMLFIDEIKVLKEEDELTEEIIEEMIKNEDIAYPVVNNNDEVVGTLSVFDLLKFYKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEU2</accession>
    <name>F6BEU2_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">DUF4040 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Metig_0117</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95678.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798287.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0117"/>
    <dbReference type="EnsemblBacteria" id="AEF95678">
        <property type="protein sequence ID" value="AEF95678"/>
        <property type="gene ID" value="Metig_0117"/>
    </dbReference>
    <dbReference type="GeneID" id="10642952"/>
    <dbReference type="KEGG" id="mig:Metig_0117"/>
    <dbReference type="HOGENOM" id="CLU_173139_2_1_2"/>
    <dbReference type="OMA" id="MAYLYYA"/>
    <dbReference type="OrthoDB" id="125479at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025383">
        <property type="entry name" value="MbhD-like_TM"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13244">
        <property type="entry name" value="DUF4040"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="6"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="29"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="53"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="domain" description="DUF4040" evidence="8">
        <location>
            <begin position="12"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF13244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AEF95678.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="80" mass="8745" checksum="2287BD3A8644C8B0" modified="2011-07-27" version="1">MEDFVAYVIMAIIVLSYISALLQKDLIKCVVFTGLGGLGLAYLYYVLLAPDVALTEAILGGAVLPAFFAFTVRRTQRIDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEU7</accession>
    <name>F6BEU7_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">3-isopropylmalate dehydratase small subunit</fullName>
            <ecNumber evidence="2">4.2.1.33</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">Alpha-IPM isomerase</fullName>
            <shortName evidence="2">IPMI</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="2">Isopropylmalate isomerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2" type="primary">leuD</name>
        <name evidence="4" type="ordered locus">Metig_0122</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>(2R,3S)-3-isopropylmalate = (2S)-2-isopropylmalate</text>
            <dbReference type="Rhea" id="RHEA:32287"/>
            <dbReference type="ChEBI" id="CHEBI:1178"/>
            <dbReference type="ChEBI" id="CHEBI:35121"/>
            <dbReference type="EC" id="4.2.1.33"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 2/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Heterodimer of LeuC and LeuD.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the LeuD family. LeuD type 2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.33" evidence="2"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95683.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798292.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0122"/>
    <dbReference type="EnsemblBacteria" id="AEF95683">
        <property type="protein sequence ID" value="AEF95683"/>
        <property type="gene ID" value="Metig_0122"/>
    </dbReference>
    <dbReference type="GeneID" id="10642957"/>
    <dbReference type="KEGG" id="mig:Metig_0122"/>
    <dbReference type="HOGENOM" id="CLU_081378_1_1_2"/>
    <dbReference type="OMA" id="DDVNTDY"/>
    <dbReference type="OrthoDB" id="86621at2157"/>
    <dbReference type="UniPathway" id="UPA00048">
        <property type="reaction ID" value="UER00071"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003861">
        <property type="term" value="F:3-isopropylmalate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009098">
        <property type="term" value="P:leucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01577">
        <property type="entry name" value="IPMI_Swivel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.19.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01032">
        <property type="entry name" value="LeuD_type2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015928">
        <property type="entry name" value="Aconitase/3IPM_dehydase_swvl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000573">
        <property type="entry name" value="AconitaseA/IPMdHydase_ssu_swvl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033940">
        <property type="entry name" value="IPMI_Swivel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011827">
        <property type="entry name" value="LeuD_type2/HacB/DmdB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00694">
        <property type="entry name" value="Aconitase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02087">
        <property type="entry name" value="LEUD_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="2" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="2" id="KW-0432">Leucine biosynthesis</keyword>
    <keyword evidence="1 2 4" id="KW-0456">Lyase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aconitase_C" evidence="3">
        <location>
            <begin position="48"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEF95683.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="168" mass="18398" checksum="9E9364733CD2F133" modified="2011-07-27" version="1">MVEKVIKGRAWVFGDNIDTDAILPARYLVYTTEEELAKFAMTGADPDFPKKVKKGDIIVGGKNFGCGSSREHAPIGLKGAGISLVIAESFARIFYRNAINIGLPLLECKDITKHVKEGDILEVDLDKGIIKNLTTGEELKAQKLPEFMMEILNDGGLMPHLKKKLGLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-02-10" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEX5</accession>
    <name>F6BEX5_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Integrase catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0152</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95711.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF95711">
        <property type="protein sequence ID" value="AEF95711"/>
        <property type="gene ID" value="Metig_0152"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0152"/>
    <dbReference type="HOGENOM" id="CLU_2044423_0_0_2"/>
    <dbReference type="OMA" id="WQMDSKI"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00665">
        <property type="entry name" value="rve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="1">
        <location>
            <begin position="145"/>
            <end position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF95711.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="269" mass="31641" checksum="2E4700FCA54D6EAA" modified="2011-07-27" version="1">MKGAKLNDKMIRKIIRWKEKGFETSRIAKKVGVTPRRVQQIWKEYRETGEIPKIKRRGRKPKKISKEEIDLVLKAVEEYKGTSAVHLEKYIESKHNIHIPHNRIYTILKEFDLIQKGKKKKKELDENLRFSLLACYASEKPKRFSASKPNEMWQMDSKIVDVDGVRYNLLLIIDDHSRFILHAGIYEEATTDVVISALEECFEKHGTPKKILTDNGTQFVPARGGISRFQKFLMEREILHIKTSIRHPQTIGKVERINREVEYRLDKFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-06-02" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEZ3</accession>
    <name>F6BEZ3_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Transcriptional regulator, XRE family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_0172</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95729.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798338.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF95729">
        <property type="protein sequence ID" value="AEF95729"/>
        <property type="gene ID" value="Metig_0172"/>
    </dbReference>
    <dbReference type="GeneID" id="10643007"/>
    <dbReference type="KEGG" id="mig:Metig_0172"/>
    <dbReference type="HOGENOM" id="CLU_133047_1_0_2"/>
    <dbReference type="OMA" id="CEKAVWY"/>
    <dbReference type="OrthoDB" id="125558at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF95729.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="100" mass="11300" checksum="8FFEB67B237F2174" modified="2011-07-27" version="1">MKTRCEVIASKVIPMIRGEIARELVNRGYAKKEVAELLGVTIAAVSQYTSEKRGATTSKRLKELVKEIVDDIEKGKISKEDLDDRFCMICSIIRKENIDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEZ9</accession>
    <name>F6BEZ9_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">dTDP-4-dehydrorhamnose reductase</fullName>
            <ecNumber evidence="2">1.1.1.133</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0178</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.1.1.133" evidence="2"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95735.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798344.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0178"/>
    <dbReference type="EnsemblBacteria" id="AEF95735">
        <property type="protein sequence ID" value="AEF95735"/>
        <property type="gene ID" value="Metig_0178"/>
    </dbReference>
    <dbReference type="GeneID" id="10643013"/>
    <dbReference type="KEGG" id="mig:Metig_0178"/>
    <dbReference type="HOGENOM" id="CLU_045518_2_1_2"/>
    <dbReference type="OMA" id="AGETTWH"/>
    <dbReference type="OrthoDB" id="43377at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008831">
        <property type="term" value="F:dTDP-4-dehydrorhamnose reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005913">
        <property type="entry name" value="dTDP_dehydrorham_reduct"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029903">
        <property type="entry name" value="RmlD-like-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10491">
        <property type="entry name" value="PTHR10491"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04321">
        <property type="entry name" value="RmlD_sub_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01214">
        <property type="entry name" value="rmlD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RmlD_sub_bind" evidence="1">
        <location>
            <begin position="9"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF95735.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="296" mass="34046" checksum="FE6AC006064A57F9" modified="2011-07-27" version="1">MEKVAVIGLGMIGYELVKKYLELDDYEVCIITRSDRGFFRDVEKYFVDITNENKIKETIKKINPDFVVNTAAMTNVDLCETEKDLAYKINALGVKYIGEICKEITCSLCHISTDYVFDGEKGNYVEEDEINPINYYGYTKAEGERLLRELDYDLISIVRISVPYCISPVKTNFFMWVLEKLRNNEVVNAVIDQWNTPTYVNELVEGIVKIYENDFNGLLHFGGGEKVSRYEFALKVAEIFGFDKSLIKPTKSSKLGWKAKRPKDTSLNSKKVEKLLKIELKDIGSALKELKNLKMR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>F6BF32</accession>
    <name>F6BF32_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Oxaloacetate decarboxylase alpha subunit</fullName>
            <ecNumber evidence="4">6.4.1.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metig_1367</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EC" id="6.4.1.1" evidence="4"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96902.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799499.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1367"/>
    <dbReference type="EnsemblBacteria" id="AEF96902">
        <property type="protein sequence ID" value="AEF96902"/>
        <property type="gene ID" value="Metig_1367"/>
    </dbReference>
    <dbReference type="GeneID" id="10644231"/>
    <dbReference type="KEGG" id="mig:Metig_1367"/>
    <dbReference type="HOGENOM" id="CLU_000395_4_2_2"/>
    <dbReference type="OMA" id="GYHALEM"/>
    <dbReference type="OrthoDB" id="19729at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008948">
        <property type="term" value="F:oxaloacetate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004736">
        <property type="term" value="F:pyruvate carboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006814">
        <property type="term" value="P:sodium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001882">
        <property type="entry name" value="Biotin_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000089">
        <property type="entry name" value="Biotin_lipoyl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003379">
        <property type="entry name" value="Carboxylase_cons_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005776">
        <property type="entry name" value="OadA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000891">
        <property type="entry name" value="PYR_CT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011053">
        <property type="entry name" value="Single_hybrid_motif"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00364">
        <property type="entry name" value="Biotin_lipoyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00682">
        <property type="entry name" value="HMGL-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02436">
        <property type="entry name" value="PYC_OADA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51230">
        <property type="entry name" value="SSF51230"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01108">
        <property type="entry name" value="oadA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00188">
        <property type="entry name" value="BIOTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50968">
        <property type="entry name" value="BIOTINYL_LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50991">
        <property type="entry name" value="PYR_CT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0092">Biotin</keyword>
    <keyword evidence="4" id="KW-0436">Ligase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pyruvate carboxyltransferase" evidence="3">
        <location>
            <begin position="2"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="domain" description="Lipoyl-binding" evidence="2">
        <location>
            <begin position="492"/>
            <end position="569"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEF96902.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="569" mass="64227" checksum="C54D0AA2EAF77B90" modified="2011-07-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>F6BF37</accession>
    <name>F6BF37_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 5">Glutamine synthetase</fullName>
            <ecNumber evidence="2 5">6.3.1.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Metig_1372</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>ATP + L-glutamate + NH4(+) = ADP + H(+) + L-glutamine + phosphate</text>
            <dbReference type="Rhea" id="RHEA:16169"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.1.2"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the glutamine synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.1.2" evidence="2 5"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96907.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799503.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1372"/>
    <dbReference type="EnsemblBacteria" id="AEF96907">
        <property type="protein sequence ID" value="AEF96907"/>
        <property type="gene ID" value="Metig_1372"/>
    </dbReference>
    <dbReference type="GeneID" id="10644237"/>
    <dbReference type="KEGG" id="mig:Metig_1372"/>
    <dbReference type="HOGENOM" id="CLU_017290_1_3_2"/>
    <dbReference type="OMA" id="PHPHEFE"/>
    <dbReference type="OrthoDB" id="47310at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004356">
        <property type="term" value="F:glutamate-ammonia ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006542">
        <property type="term" value="P:glutamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008147">
        <property type="entry name" value="Gln_synt_b-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036651">
        <property type="entry name" value="Gln_synt_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014746">
        <property type="entry name" value="Gln_synth/guanido_kin_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008146">
        <property type="entry name" value="Gln_synth_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027303">
        <property type="entry name" value="Gln_synth_gly_rich_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004809">
        <property type="entry name" value="Gln_synth_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027302">
        <property type="entry name" value="Gln_synth_N_conserv_site"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00120">
        <property type="entry name" value="Gln-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03951">
        <property type="entry name" value="Gln-synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01230">
        <property type="entry name" value="Gln-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54368">
        <property type="entry name" value="SSF54368"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55931">
        <property type="entry name" value="SSF55931"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00653">
        <property type="entry name" value="GlnA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00180">
        <property type="entry name" value="GLNA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00181">
        <property type="entry name" value="GLNA_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0436">Ligase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gln-synt_C" evidence="6">
        <location>
            <begin position="110"/>
            <end position="359"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU000384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU004356"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM01230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEF96907.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="448" mass="50782" checksum="842861DE2F495CB7" modified="2011-07-27" version="1">MSDIEKVIQFVKENKVKFLRLQFVDILGFPKNVAYPIKEGEKGLDELRDILENGVYFDGSSIAGFVGIQESDMILKPDVSTFSVLPWRPNEKSVARVICDVYRTEGKPFEGDPRSCLKKILNELKEEMNGEFFVGPEPEFFLLKRDPHNPHKWIPADDGGYFDVEPLDQAPDIRRDIVLALENLGFHVEASHHEVAPGQHEVDFKFDNALKTADSVVTFKTTIKAIAQKHGLKATFMPKPFFGMNGNGMHCHQSVWFNGEPSFYDPDAKYQLSETCMQYIAGILNHAKAIVAITNPTVNSYKRLVPGYEAPVNIAWANKNRSAIIRVPAARGKGTRIEFRAPDPTCNPYLAFTVMLAAGLDGIKKKMEAPEPVEKNIFKMSEEEKKQLGIESVPSNLKEALDELECDEVLQKALGKHIYENFMEIKRAEWDSFRTSITNWELDNYLVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F6BF55</accession>
    <name>F6BF55_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">4Fe-4S ferredoxin iron-sulfur binding domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1390</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96925.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799521.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1390"/>
    <dbReference type="EnsemblBacteria" id="AEF96925">
        <property type="protein sequence ID" value="AEF96925"/>
        <property type="gene ID" value="Metig_1390"/>
    </dbReference>
    <dbReference type="GeneID" id="10644263"/>
    <dbReference type="KEGG" id="mig:Metig_1390"/>
    <dbReference type="HOGENOM" id="CLU_139698_5_3_2"/>
    <dbReference type="OMA" id="CARMCPD"/>
    <dbReference type="OrthoDB" id="124457at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12838">
        <property type="entry name" value="Fer4_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="37"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96925.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="64" mass="7207" checksum="F12DE18131839E2B" modified="2011-07-27" version="1">MISINENFCKGCDICIEVCPKKVFEKSKKLNKKGIYPPIPVNEDKCVKCMLCVLQCPDQAISVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>F6BF59</accession>
    <name>F6BF59_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1394</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96929.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799525.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96929">
        <property type="protein sequence ID" value="AEF96929"/>
        <property type="gene ID" value="Metig_1394"/>
    </dbReference>
    <dbReference type="GeneID" id="10644267"/>
    <dbReference type="KEGG" id="mig:Metig_1394"/>
    <dbReference type="HOGENOM" id="CLU_2257406_0_0_2"/>
    <dbReference type="OMA" id="LEVWIVY"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="34"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="61"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96929.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="109" mass="12446" checksum="3B3AEB701E4185CB" modified="2011-07-27" version="1">MGFLKTLFKFLVLVFVGFIFTLIGVGLKGILGLICYAISFIIFLVAYAYLIFKIVTWKYFWYIAFPVLIIILILAPIDGIILTIALIILRNSLIKLKNVFEGEDENNHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-10-19" modified="2022-01-19" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>G1UBJ1</accession>
    <name>G1UBJ1_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">50S ribosomal protein L10</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">Acidic ribosomal protein P0 homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2" type="primary">rpl10</name>
        <name evidence="2" type="synonym">rplP0</name>
        <name evidence="5" type="ordered locus">Metig_0942</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Forms part of the ribosomal stalk, playing a central role in the interaction of the ribosome with GTP-bound translation factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Part of the 50S ribosomal subunit. Forms part of the ribosomal stalk which helps the ribosome interact with GTP-bound translation factors. Forms a heptameric L10(L12)2(L12)2(L12)2 complex, where L10 forms an elongated spine to which the L12 dimers bind in a sequential fashion.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the universal ribosomal protein uL10 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96485.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799087.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0942"/>
    <dbReference type="EnsemblBacteria" id="AEF96485">
        <property type="protein sequence ID" value="AEF96485"/>
        <property type="gene ID" value="Metig_0942"/>
    </dbReference>
    <dbReference type="GeneID" id="10643788"/>
    <dbReference type="KEGG" id="mig:Metig_0942"/>
    <dbReference type="HOGENOM" id="CLU_053173_0_0_2"/>
    <dbReference type="OMA" id="MAHVAEW"/>
    <dbReference type="OrthoDB" id="73593at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070180">
        <property type="term" value="F:large ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1730">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.105.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00280">
        <property type="entry name" value="Ribosomal_L10_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022909">
        <property type="entry name" value="50S_L10_arch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043141">
        <property type="entry name" value="Ribosomal_L10-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001790">
        <property type="entry name" value="Ribosomal_L10P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043164">
        <property type="entry name" value="RL10_insert_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040637">
        <property type="entry name" value="RL10P_insert"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00466">
        <property type="entry name" value="Ribosomal_L10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17777">
        <property type="entry name" value="RL10P_insert"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160369">
        <property type="entry name" value="SSF160369"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 5" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="2" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="RL10P_insert" evidence="4">
        <location>
            <begin position="119"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="303"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="314"/>
            <end position="331"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF17777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEF96485.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="342" mass="36901" checksum="8E113F19E99A359D" modified="2011-10-19" version="1">MTSAVATQRKIAPWKIEEVKTLKELLKSKPVVAIVDMMEVPARQLQEIRDKIRGKMILRMSRNSLIIRAIKEVAEETNNPEFAKLADYVEKGAAILVTDMNPFKLFKELEASKTPAPIKGGAIAPCDIKVQKGSTGMPPGPFLGELKSVGIPAAIEKGKIVIKEDKVVVKAGEVVSHKLATILSTLGIKPIKVGLNLLAAYEDGIIYTPDVLKIDEEEFISKIQNAYMHAVNLSVNAAIPTKATIEMLIQKAFLNARAVSVESAFLTDKTASDIIGKAYAQMLALAGKLSDDALDDDLRERLSASASAAAPAPAAEEKKEEEKKEEKKDDTATAAAGLALLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>F6BA78</accession>
    <name>F6BA78_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">CRISPR-associated protein Csc1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_0211</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95768.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798377.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0211"/>
    <dbReference type="EnsemblBacteria" id="AEF95768">
        <property type="protein sequence ID" value="AEF95768"/>
        <property type="gene ID" value="Metig_0211"/>
    </dbReference>
    <dbReference type="GeneID" id="10643046"/>
    <dbReference type="KEGG" id="mig:Metig_0211"/>
    <dbReference type="HOGENOM" id="CLU_1259135_0_0_2"/>
    <dbReference type="OMA" id="IGVGREC"/>
    <dbReference type="OrthoDB" id="64499at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017576">
        <property type="entry name" value="CRISPR-assoc_prot_Csc1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03159">
        <property type="entry name" value="cas_Csc1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF95768.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="210" mass="24677" checksum="8ED6DB13879F9AFB" modified="2011-07-27" version="1">MLRHYKITLLSPLFCYNKTEGGVASTEPFIGDIALDYALNFALAKKREYTYQIKDKPDYDEIKEFGFIWTIGRPIYYEKTPIFARKTSEISDYMVRRDIIEQMGKGLFKNYFHIQGIKEGSVFEASLLTFEDIKLPKTFTLRIGVGRECLLLFEEKKEKPEEIWLNLYTIKKIFGKDVKLKESQMLRFVLSHYIIGTGFKVEDLEKIFSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>F6BA94</accession>
    <name>F6BA94_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Nucleotide sugar dehydrogenase</fullName>
            <ecNumber evidence="7">1.1.1.22</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Metig_0227</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.1.1.22" evidence="7"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95784.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798393.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0227"/>
    <dbReference type="EnsemblBacteria" id="AEF95784">
        <property type="protein sequence ID" value="AEF95784"/>
        <property type="gene ID" value="Metig_0227"/>
    </dbReference>
    <dbReference type="GeneID" id="10643062"/>
    <dbReference type="KEGG" id="mig:Metig_0227"/>
    <dbReference type="HOGENOM" id="CLU_320207_0_0_2"/>
    <dbReference type="OMA" id="IKWEFLK"/>
    <dbReference type="OrthoDB" id="47911at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003979">
        <property type="term" value="F:UDP-glucose 6-dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016539">
        <property type="term" value="P:intein-mediated protein splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.28.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003586">
        <property type="entry name" value="Hint_dom_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003587">
        <property type="entry name" value="Hint_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036844">
        <property type="entry name" value="Hint_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027434">
        <property type="entry name" value="Homing_endonucl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006142">
        <property type="entry name" value="INTEIN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030934">
        <property type="entry name" value="Intein_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004042">
        <property type="entry name" value="Intein_endonuc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006141">
        <property type="entry name" value="Intein_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004860">
        <property type="entry name" value="LAGLIDADG_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017476">
        <property type="entry name" value="UDP-Glc/GDP-Man"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014027">
        <property type="entry name" value="UDP-Glc/GDP-Man_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036220">
        <property type="entry name" value="UDP-Glc/GDP-Man_DH_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014026">
        <property type="entry name" value="UDP-Glc/GDP-Man_DH_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001732">
        <property type="entry name" value="UDP-Glc/GDP-Man_DH_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14528">
        <property type="entry name" value="LAGLIDADG_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00984">
        <property type="entry name" value="UDPG_MGDP_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03720">
        <property type="entry name" value="UDPG_MGDP_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03721">
        <property type="entry name" value="UDPG_MGDP_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00379">
        <property type="entry name" value="INTEIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00305">
        <property type="entry name" value="HintC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00306">
        <property type="entry name" value="HintN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00984">
        <property type="entry name" value="UDPG_MGDP_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="SSF48179"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51294">
        <property type="entry name" value="SSF51294"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52413">
        <property type="entry name" value="SSF52413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55608">
        <property type="entry name" value="SSF55608"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01445">
        <property type="entry name" value="intein_Nterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03026">
        <property type="entry name" value="NDP-sugDHase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50818">
        <property type="entry name" value="INTEIN_C_TER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50819">
        <property type="entry name" value="INTEIN_ENDONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50817">
        <property type="entry name" value="INTEIN_N_TER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0068">Autocatalytic cleavage</keyword>
    <keyword evidence="4" id="KW-0520">NAD</keyword>
    <keyword evidence="3 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2" id="KW-0651">Protein splicing</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DOD-type homing endonuclease" evidence="6">
        <location>
            <begin position="472"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="domain" description="INTEIN_C_TER" evidence="5">
        <location>
            <begin position="697"/>
            <end position="719"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50818"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEF95784.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="900" mass="102741" checksum="4EBFEEF3B9913B0F" modified="2011-07-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAA7</accession>
    <name>F6BAA7_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Glycosyl transferase group 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_0240</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95797.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0240"/>
    <dbReference type="EnsemblBacteria" id="AEF95797">
        <property type="protein sequence ID" value="AEF95797"/>
        <property type="gene ID" value="Metig_0240"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0240"/>
    <dbReference type="HOGENOM" id="CLU_009583_37_1_2"/>
    <dbReference type="OMA" id="YTWENYG"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028098">
        <property type="entry name" value="Glyco_trans_4-like_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13439">
        <property type="entry name" value="Glyco_transf_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glyco_trans_4-like_N" evidence="2">
        <location>
            <begin position="82"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="domain" description="Glycos_transf_1" evidence="1">
        <location>
            <begin position="210"/>
            <end position="355"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF95797.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="386" mass="45121" checksum="FC2677F347B52E4F" modified="2011-07-27" version="1">MILHIINSDFGVKNTIGIRSYYITKEANNYFYIFCRNYNKNINLHNVKIKQIFPFAHYVMKFLTAIPIYIKKNFPSNEIKNNIFEFALISKVKDLNLKNVDVIHSWEFLPNFYRYIKSKNPDIKIIQDVPMALPNVLKDIKNYEKLFGKVEIPQYVKKSLNYIDYYISPSEFVKKSLINEGIDENKIFVVPFGVDVGRFKPIEKDHFGTFKVAFSGNVNNRKGILYLIQAWKELNLKNAELNLYGIVYPEVRKYLKNANKYNIKLYGFVKNIELELPKNHIYVFPSLLEGSAKSIYEALACGLPVITTENSGSVVEDGKDGFIIPVQNVEAIKEKILFFYENRNEIVKFGKNARKKAEKYTWENYGKKVNEIYELVNNHEQNFICP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAB5</accession>
    <name>F6BAB5_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Glycosyl transferase group 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_0248</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95805.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798414.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0248"/>
    <dbReference type="EnsemblBacteria" id="AEF95805">
        <property type="protein sequence ID" value="AEF95805"/>
        <property type="gene ID" value="Metig_0248"/>
    </dbReference>
    <dbReference type="GeneID" id="10643083"/>
    <dbReference type="KEGG" id="mig:Metig_0248"/>
    <dbReference type="HOGENOM" id="CLU_009583_2_5_2"/>
    <dbReference type="OMA" id="MTRTRFF"/>
    <dbReference type="OrthoDB" id="83551at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028098">
        <property type="entry name" value="Glyco_trans_4-like_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13439">
        <property type="entry name" value="Glyco_transf_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glyco_trans_4-like_N" evidence="2">
        <location>
            <begin position="17"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="domain" description="Glycos_transf_1" evidence="1">
        <location>
            <begin position="199"/>
            <end position="367"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF95805.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="392" mass="45683" checksum="630AA0B40FEA02F0" modified="2011-07-27" version="1">MKKIKLIIFPGYYLPHIGGLETHVDEFVKYLSKDKNFDITIFAPNIPKSKEFEVKYNNVKIIRYPAFEIIPNYPVPNMLSPKFWKLFFNLYKIDFDIVMTRTRFFSNTLLGFLFAKLRFNKKILIHVEHGSAFIKVQNEITNKLSYLYDKTIGKLIFKKSDYVIAISKAVKEFILNNFIKREIPIIYRGIEFEKIEKIKENEEIKNKFKGKLKLCFVGRLYKWKGVENIIKAYKILPENIKKDAVLIIVGYGEDLERLKKLANEYLNNGIYFLGKKDFEEAIGILKVCDIYIHSAYPGGGLSTSLLEAMVCGRAIVASPYEGGDEVIIDEKTGILLEDNNPEEIKKGIIKLIENKELIKIYGENAKKFVKENFSWDKAIEKYKEIFKELLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAC6</accession>
    <name>F6BAC6_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">DNA polymerase beta domain protein region</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0260</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95816.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798425.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF95816">
        <property type="protein sequence ID" value="AEF95816"/>
        <property type="gene ID" value="Metig_0260"/>
    </dbReference>
    <dbReference type="GeneID" id="10643095"/>
    <dbReference type="KEGG" id="mig:Metig_0260"/>
    <dbReference type="HOGENOM" id="CLU_130257_10_3_2"/>
    <dbReference type="OMA" id="YMNLKFY"/>
    <dbReference type="OrthoDB" id="108083at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002934">
        <property type="entry name" value="Polymerase_NTP_transf_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01909">
        <property type="entry name" value="NTP_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="SSF81301"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NTP_transf_2" evidence="1">
        <location>
            <begin position="13"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF95816.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="97" mass="11431" checksum="46F550861BD0C565" modified="2011-07-27" version="1">MKTLNEIKKILLEHKQELKEKYKVKSIAIFGSYARGEQTEKSDIDILVEFYEPIGLKIVDLRDYLEEILNIKVDLITKNSIQNPYIKKSIEEDLIYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAJ2</accession>
    <name>F6BAJ2_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_1471</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97005.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799601.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF97005">
        <property type="protein sequence ID" value="AEF97005"/>
        <property type="gene ID" value="Metig_1471"/>
    </dbReference>
    <dbReference type="GeneID" id="10644344"/>
    <dbReference type="KEGG" id="mig:Metig_1471"/>
    <dbReference type="HOGENOM" id="CLU_1507388_0_0_2"/>
    <dbReference type="OMA" id="CSCITEK"/>
    <dbReference type="OrthoDB" id="119790at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF97005.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="178" mass="21495" checksum="7AC5295857C632D3" modified="2011-07-27" version="1">MTYKLLYVFVEGNDDERFFERVIKPLLEKKYDHVQIIKYAEEKDEKIINWIKSIESMDWADYIYVADINNSPCITRRKEKIKQRYKNVDNNKIIIVIKKIESWYIAGISPELAKKLKIKLTHNIDANTITKEQFNNLIPKNDSRINFMIEILKHYSIEIARKKNKSFDYFMRKFIDAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAL0</accession>
    <name>F6BAL0_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Cell wall binding repeat 2-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_1489</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97023.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799618.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1489"/>
    <dbReference type="EnsemblBacteria" id="AEF97023">
        <property type="protein sequence ID" value="AEF97023"/>
        <property type="gene ID" value="Metig_1489"/>
    </dbReference>
    <dbReference type="GeneID" id="10644362"/>
    <dbReference type="KEGG" id="mig:Metig_1489"/>
    <dbReference type="HOGENOM" id="CLU_840944_0_0_2"/>
    <dbReference type="OMA" id="EYAYKIN"/>
    <dbReference type="OrthoDB" id="82075at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007253">
        <property type="entry name" value="Cell_wall-bd_2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04122">
        <property type="entry name" value="CW_binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF97023.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="337" mass="39035" checksum="9EDD3DC4030F02CF" modified="2011-07-27" version="1">MRYLKLITIILILIPCIDALTIGEKPSLVDTVIITNDNWADCLAVANYAYQSDAIILQTEKDNLNPKVEEIIKIVNPKNIIIIGGPEAISENVERKLEKYAPTVRIWGNDRAETFEKIIDYQLKNKKVSNESIYLNYCLVNGYDFDDVVTVSNFYVPYYASLKILNPKYTIRVYENDTVKIYEMHKNHKIFVGEYSKDCVFEFPGKIIIFKKPKYNVKYCYNNNLCKFGYEKIEVRDFREGILITKNTPTAMLLSKYLKVPVILDGHTIIYLEDDPIESSIAVAVDILVLKKAKELYENSGNAKQAIDEAKTQLWAKKLPVEKYNIPYEYARRYIEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAM5</accession>
    <name>F6BAM5_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phosphate-specific transport system accessory protein PhoU</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_0283</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Plays a role in the regulation of phosphate uptake.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the PhoU family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95839.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798448.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0283"/>
    <dbReference type="EnsemblBacteria" id="AEF95839">
        <property type="protein sequence ID" value="AEF95839"/>
        <property type="gene ID" value="Metig_0283"/>
    </dbReference>
    <dbReference type="GeneID" id="10643118"/>
    <dbReference type="KEGG" id="mig:Metig_0283"/>
    <dbReference type="HOGENOM" id="CLU_078518_3_0_2"/>
    <dbReference type="OMA" id="WKHGIET"/>
    <dbReference type="OrthoDB" id="76473at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030643">
        <property type="term" value="P:cellular phosphate ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045936">
        <property type="term" value="P:negative regulation of phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006817">
        <property type="term" value="P:phosphate ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.220">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028366">
        <property type="entry name" value="P_transport_PhoU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038078">
        <property type="entry name" value="PhoU-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026022">
        <property type="entry name" value="PhoU_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42930">
        <property type="entry name" value="PTHR42930"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01895">
        <property type="entry name" value="PhoU"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003107">
        <property type="entry name" value="PhoU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02135">
        <property type="entry name" value="phoU_full"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1" id="KW-0592">Phosphate transport</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="PhoU" evidence="2">
        <location>
            <begin position="18"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="domain" description="PhoU" evidence="2">
        <location>
            <begin position="119"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRNR" id="PIRNR003107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF95839.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="228" mass="26500" checksum="78D03D08FFD1ED8A" modified="2011-07-27" version="1">MTREAFYTKLEEIEEDVLKMGELCAKATTNAVKAFLDNDKELAKKIRKEDDEIDKMEIDIEEKCISLIALQHPVASDLREILTIIKIISKLEKVGDNASKIAKIVLKSELNIKRENEILNIMVDYLESMLRDALIAFKTKNEALAREVYNRDKKIDKLYEQLYREMISYMIENPKNITMATETIFVAKYLERNGNIIASIGDRVVYMITGERIKEEELEEKLEKDKNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAN0</accession>
    <name>F6BAN0_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Carbon monoxide dehydrogenase</fullName>
            <ecNumber evidence="10">1.2.7.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">Metig_0288</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">CODH oxidizes carbon monoxide coupled, via CooF, to the reduction of a hydrogen cation by a hydrogenase (possibly CooH).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>CO + H2O + 2 oxidized [2Fe-2S]-[ferredoxin] = CO2 + 2 H(+) + 2 reduced [2Fe-2S]-[ferredoxin]</text>
            <dbReference type="Rhea" id="RHEA:21040"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:17245"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="EC" id="1.2.7.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>[Ni-Fe-S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:60400"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the Ni-containing carbon monoxide dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.2.7.4" evidence="10"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95844.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798453.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0288"/>
    <dbReference type="EnsemblBacteria" id="AEF95844">
        <property type="protein sequence ID" value="AEF95844"/>
        <property type="gene ID" value="Metig_0288"/>
    </dbReference>
    <dbReference type="GeneID" id="10643123"/>
    <dbReference type="KEGG" id="mig:Metig_0288"/>
    <dbReference type="HOGENOM" id="CLU_030631_0_0_2"/>
    <dbReference type="OMA" id="MCCTGNE"/>
    <dbReference type="OrthoDB" id="5135at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018492">
        <property type="term" value="F:carbon-monoxide dehydrogenase (acceptor) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043885">
        <property type="term" value="F:carbon-monoxide dehydrogenase (ferredoxin) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016151">
        <property type="term" value="F:nickel cation binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006091">
        <property type="term" value="P:generation of precursor metabolites and energy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01915">
        <property type="entry name" value="CODH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2030">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016101">
        <property type="entry name" value="CO_DH_a-bundle"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010047">
        <property type="entry name" value="CODH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004137">
        <property type="entry name" value="HCP/CODH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016099">
        <property type="entry name" value="Prismane-like_a/b-sand"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011254">
        <property type="entry name" value="Prismane-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30109">
        <property type="entry name" value="PTHR30109"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03063">
        <property type="entry name" value="Prismane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005023">
        <property type="entry name" value="CODH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56821">
        <property type="entry name" value="SSF56821"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01702">
        <property type="entry name" value="CO_DH_cata"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 11" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="8 10 11" id="KW-0408">Iron</keyword>
    <keyword evidence="9 10 11" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="7 10 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0533">Nickel</keyword>
    <keyword evidence="10 12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="11">
        <location>
            <position position="42"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="50"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="51"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="54"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="59"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="73"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="265"/>
        </location>
        <ligand>
            <name>[Ni-4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:47739"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="301"/>
        </location>
        <ligand>
            <name>[Ni-4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:47739"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="339"/>
        </location>
        <ligand>
            <name>[Ni-4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:47739"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="446"/>
        </location>
        <ligand>
            <name>[Ni-4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:47739"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="476"/>
        </location>
        <ligand>
            <name>[Ni-4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:47739"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="517"/>
        </location>
        <ligand>
            <name>[Ni-4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:47739"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00002452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010689"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR005023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR005023-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AEF95844.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="625" mass="66840" checksum="D826198008245424" modified="2011-07-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAT5</accession>
    <name>F6BAT5_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Methanogenesis marker protein 15</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metig_0343</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95899.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798508.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0343"/>
    <dbReference type="EnsemblBacteria" id="AEF95899">
        <property type="protein sequence ID" value="AEF95899"/>
        <property type="gene ID" value="Metig_0343"/>
    </dbReference>
    <dbReference type="GeneID" id="10643180"/>
    <dbReference type="KEGG" id="mig:Metig_0343"/>
    <dbReference type="HOGENOM" id="CLU_666680_0_0_2"/>
    <dbReference type="OMA" id="ATCFRCA"/>
    <dbReference type="OrthoDB" id="22876at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002731">
        <property type="entry name" value="ATPase_BadF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008275">
        <property type="entry name" value="CoA_E_activase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017676">
        <property type="entry name" value="Methan_mark_15"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01869">
        <property type="entry name" value="BcrAD_BadFG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="SSF53067"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00241">
        <property type="entry name" value="CoA_E_activ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03286">
        <property type="entry name" value="methan_mark_15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0408">Iron</keyword>
    <keyword evidence="2" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BcrAD_BadFG" evidence="3">
        <location>
            <begin position="148"/>
            <end position="402"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEF95899.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="412" mass="44461" checksum="962F153368C8AE60" modified="2011-07-27" version="1">MVKIAELTCGAEYSGVQKEIEKAAAAVGGEIIFPEVDLDYIDKVEEYLGFEVASANLKLMFARAMSIIEGNTDAEAVFIATCFRCAEGALVRNEVRRLIQQHTDLPVVMYSFTERTKASELLTRMEALATIVERKSLLARKKQEGISLGIDSGSTTTKAVVMQDNEVVGTGWVYTRDVIESAKEAVDNALKEAGLKIEDIETIGTTGYGRHTIGEYFKADLIQEELTVNSKGAAFLADKQEGEATVIDIGGMDNKAISLNNAIPDSFTMGGICAGASGRFFEITARRLGVSIQELGELAAKGDWRKINMNSYCIVFGIQDLVTALAEGAKAEDVAAAAAHSVAEQIYEQQLQEVDVRDPVILVGGSSLLKGMVLALEEILGRKIIVPPYSQHIGAVGAALLASGFRYMKKKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAV4</accession>
    <name>F6BAV4_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">TatD-related deoxyribonuclease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1507</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the metallo-dependent hydrolases superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97041.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF97041">
        <property type="protein sequence ID" value="AEF97041"/>
        <property type="gene ID" value="Metig_1507"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_1507"/>
    <dbReference type="HOGENOM" id="CLU_061552_1_0_2"/>
    <dbReference type="OMA" id="WAAFTIY"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001130">
        <property type="entry name" value="TatD-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012022">
        <property type="entry name" value="UCP005295"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42658">
        <property type="entry name" value="PTHR42658"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01026">
        <property type="entry name" value="TatD_DNase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005295">
        <property type="entry name" value="UCP005295_TatD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="SSF51556"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRNR" id="PIRNR005295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF97041.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="253" mass="28379" checksum="DA4175F65CD46B97" modified="2011-07-27" version="1">MIDAHIHADTRPYEDFEMMALCMDGAITLAHDPFEMKSSDAWVSHVERLINNDVKRAQENGLKLFVCVGIHPRAIPKDYENAIEKIKDFVKNDVVVGIGEIGLEKATKEEKDVFIKQLLLAKELDLPAVVHTPRRNKEEITKIIIEEINSLDLKNEKIVIDHCNKNTVKDVLDIGCYAGLTVQPSKLTPMEAVEIVKEFGGEKILLDSDSSSAPSDILSVPKTVLKMKLNDVDKEIINLVSHENAKKLFNLTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-06-02" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBC4</accession>
    <name>F6BBC4_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1598</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97131.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF97131">
        <property type="protein sequence ID" value="AEF97131"/>
        <property type="gene ID" value="Metig_1598"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_1598"/>
    <dbReference type="HOGENOM" id="CLU_165881_0_0_2"/>
    <dbReference type="OMA" id="EDIMLAE"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="91"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF97131.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="120" mass="14128" checksum="100829912BACEEF3" modified="2011-07-27" version="1">MCGIMAVALPKLYELILKKVKNEKEAKEIYDIILELNKENKIIIKNELKDELKNELATKEDIYILEEKMNVMEERLMRYVDNKFNQLDKKITVGFVIIILLYILTNPNAIELIKLLFGLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBD7</accession>
    <name>F6BBD7_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1611</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97144.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799737.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1611"/>
    <dbReference type="EnsemblBacteria" id="AEF97144">
        <property type="protein sequence ID" value="AEF97144"/>
        <property type="gene ID" value="Metig_1611"/>
    </dbReference>
    <dbReference type="GeneID" id="10644484"/>
    <dbReference type="KEGG" id="mig:Metig_1611"/>
    <dbReference type="HOGENOM" id="CLU_174513_1_0_2"/>
    <dbReference type="OMA" id="CINFCIA"/>
    <dbReference type="OrthoDB" id="120584at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019214">
        <property type="entry name" value="DUF2109_membrane"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09882">
        <property type="entry name" value="DUF2109"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="29"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="53"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF97144.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="81" mass="8849" checksum="10649406D3E438D6" modified="2011-07-27" version="1">MDLSSIIIGVIAVICGLRVFLTQSRARKLLYLCCLNFCIAALIALYIKSPMGSLAAIVYLISSTLSSNAIAYTLEQMKKME</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBD8</accession>
    <name>F6BBD8_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1612</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97145.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799738.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1612"/>
    <dbReference type="EnsemblBacteria" id="AEF97145">
        <property type="protein sequence ID" value="AEF97145"/>
        <property type="gene ID" value="Metig_1612"/>
    </dbReference>
    <dbReference type="GeneID" id="10644485"/>
    <dbReference type="KEGG" id="mig:Metig_1612"/>
    <dbReference type="HOGENOM" id="CLU_170806_0_0_2"/>
    <dbReference type="OMA" id="LEIGMIG"/>
    <dbReference type="OrthoDB" id="124851at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019213">
        <property type="entry name" value="DUF2108_membrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011308">
        <property type="entry name" value="Prd_NiFe_hyd_3_EhaD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09881">
        <property type="entry name" value="DUF2108"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006581">
        <property type="entry name" value="EhaD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="29"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="57"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF97145.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="92" mass="9992" checksum="360CDE56A7F690CF" modified="2011-07-27" version="1">MDILPTVSAICCVLGAIGVIVHTNPINKIIMFAFLESGLIGLIVSCYYLDVAIVSSILEPIGTVVLLLGLMKYEVVKKSKKKYGRKLPILTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBH5</accession>
    <name>F6BBH5_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Succinate--CoA ligase [ADP-forming] subunit beta</fullName>
            <ecNumber evidence="6">6.2.1.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Succinyl-CoA synthetase subunit beta</fullName>
            <shortName evidence="6">SCS-beta</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">sucC</name>
        <name evidence="9" type="ordered locus">Metig_1650</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Succinyl-CoA synthetase functions in the citric acid cycle (TCA), coupling the hydrolysis of succinyl-CoA to the synthesis of either ATP or GTP and thus represents the only step of substrate-level phosphorylation in the TCA. The beta subunit provides nucleotide specificity of the enzyme and binds the substrate succinate, while the binding sites for coenzyme A and phosphate are found in the alpha subunit.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>ATP + CoA + succinate = ADP + phosphate + succinyl-CoA</text>
            <dbReference type="Rhea" id="RHEA:17661"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57292"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.2.1.5"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="6">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="6">Carbohydrate metabolism; tricarboxylic acid cycle; succinate from succinyl-CoA (ligase route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Heterotetramer of two alpha and two beta subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the succinate/malate CoA ligase beta subunit family.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.5" evidence="6"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97182.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799773.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1650"/>
    <dbReference type="EnsemblBacteria" id="AEF97182">
        <property type="protein sequence ID" value="AEF97182"/>
        <property type="gene ID" value="Metig_1650"/>
    </dbReference>
    <dbReference type="GeneID" id="10644523"/>
    <dbReference type="KEGG" id="mig:Metig_1650"/>
    <dbReference type="HOGENOM" id="CLU_037430_0_2_2"/>
    <dbReference type="OMA" id="ITACDEV"/>
    <dbReference type="OrthoDB" id="72275at2157"/>
    <dbReference type="UniPathway" id="UPA00223">
        <property type="reaction ID" value="UER00999"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004775">
        <property type="term" value="F:succinate-CoA ligase (ADP-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.261">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00558">
        <property type="entry name" value="Succ_CoA_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013650">
        <property type="entry name" value="ATP-grasp_succ-CoA_synth-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005811">
        <property type="entry name" value="CoA_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017866">
        <property type="entry name" value="Succ-CoA_synthase_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005809">
        <property type="entry name" value="Succ_CoA_synthase_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016102">
        <property type="entry name" value="Succinyl-CoA_synth-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11815">
        <property type="entry name" value="PTHR11815"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08442">
        <property type="entry name" value="ATP-grasp_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00549">
        <property type="entry name" value="Ligase_CoA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001554">
        <property type="entry name" value="SucCS_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52210">
        <property type="entry name" value="SSF52210"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01016">
        <property type="entry name" value="sucCoAbeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01217">
        <property type="entry name" value="SUCCINYL_COA_LIG_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 6 9" id="KW-0436">Ligase</keyword>
    <keyword evidence="5 6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 6 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 6" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="ATP-grasp" evidence="8">
        <location>
            <begin position="9"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding; shared with subunit alpha" evidence="6">
        <location>
            <begin position="305"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="45"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="52"/>
            <end position="54"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="91"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="94"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="99"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="191"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="205"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="248"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared with subunit alpha</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AEF97182.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="367" mass="41127" checksum="6BC5F7FA8CDF671A" modified="2011-07-27" version="1">MKLHEYEAKNIFKEYGIPIPEGFIISKEEISKIDDIGINKPVVVKAQVLVGGRGKAGGILFADNKEELKEKINELFSKEIKGEEVEKVLIEEKLPIKKEYYLGIVIDRTEKRPAIMFSTEGGVDIEEVAKRNPEKIVKYHIEPRKEFLPYSARWILKHAGIPSKEIPIVADVMYKLYKVFKEMDAVLAEINPLVITEDGKVYAADAVLNVDDDAYYRHDYSKFEEFGKKEKLEFAYVELDGDIGVIGNGAGLTLASMDIIKEFGGNPACFLDIGGGASAEIVKKALKKVLENPKVRGVFINILGGITRCDEVAKGIVEILKEHPNVKFAVRMMGTNEEEGRKILEENGIPYETSMEEAARKLMENLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBH9</accession>
    <name>F6BBH9_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_1654</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97186.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799777.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1654"/>
    <dbReference type="EnsemblBacteria" id="AEF97186">
        <property type="protein sequence ID" value="AEF97186"/>
        <property type="gene ID" value="Metig_1654"/>
    </dbReference>
    <dbReference type="GeneID" id="10644527"/>
    <dbReference type="KEGG" id="mig:Metig_1654"/>
    <dbReference type="HOGENOM" id="CLU_2243898_0_0_2"/>
    <dbReference type="OMA" id="CCILHAY"/>
    <dbReference type="OrthoDB" id="99408at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF97186.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="107" mass="12766" checksum="AD39A2B1C4F2746B" modified="2011-07-27" version="1">MKTLRFKKDKVIKISEELFPDELCERCGRCCILHAYKTENGLEVIYCQHLDKETKLCSVYKNRFEHGCLTVMEGIMAGVFPKDCPYVKDLESYEEPWFYRLIREENE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBI7</accession>
    <name>F6BBI7_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uroporphyrin-III C-methyltransferase</fullName>
            <ecNumber evidence="6">2.1.1.107</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Metig_0440</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the precorrin methyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.107" evidence="6"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95996.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798605.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0440"/>
    <dbReference type="EnsemblBacteria" id="AEF95996">
        <property type="protein sequence ID" value="AEF95996"/>
        <property type="gene ID" value="Metig_0440"/>
    </dbReference>
    <dbReference type="GeneID" id="10643277"/>
    <dbReference type="KEGG" id="mig:Metig_0440"/>
    <dbReference type="HOGENOM" id="CLU_011276_7_0_2"/>
    <dbReference type="OMA" id="TRPEQEE"/>
    <dbReference type="OrthoDB" id="67633at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004851">
        <property type="term" value="F:uroporphyrin-III C-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019354">
        <property type="term" value="P:siroheme biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11642">
        <property type="entry name" value="SUMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.950.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1010.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000878">
        <property type="entry name" value="4pyrrol_Mease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035996">
        <property type="entry name" value="4pyrrol_Methylase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014777">
        <property type="entry name" value="4pyrrole_Mease_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014776">
        <property type="entry name" value="4pyrrole_Mease_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006366">
        <property type="entry name" value="CobA/CysG_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003043">
        <property type="entry name" value="Uropor_MeTrfase_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00590">
        <property type="entry name" value="TP_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53790">
        <property type="entry name" value="SSF53790"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01469">
        <property type="entry name" value="cobA_cysG_Cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00839">
        <property type="entry name" value="SUMT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00840">
        <property type="entry name" value="SUMT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4 6" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2 4 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="TP_methylase" evidence="5">
        <location>
            <begin position="2"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEF95996.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="240" mass="26175" checksum="3E1B54244C3CC02A" modified="2011-07-27" version="1">MKVILVGAGPGDEELITLKGLKAIQNADVIVYDDLVGKGLLKYAKESAELIYVGKRKGKHSHKQEEINKILVEKAKEGKIVVRLKGGDPFVFGRGGEEILELKKHDIPYEVIPGITSAIAVPEVAGIPVTHRKVATSFTVVTGHEAEDKEHQQVKLSELNADTIIILMGITNLEKHVKELLKNPKRNENTPVAIITDGTTEKQKVVKGTLGNIVEKAKEHDIKPPGVIVVGEVVNVLDKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBI8</accession>
    <name>F6BBI8_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Transcriptional regulator NikR, CopG family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Metig_0441</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the transcriptional regulatory CopG/NikR family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95997.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798606.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0441"/>
    <dbReference type="EnsemblBacteria" id="AEF95997">
        <property type="protein sequence ID" value="AEF95997"/>
        <property type="gene ID" value="Metig_0441"/>
    </dbReference>
    <dbReference type="GeneID" id="10643278"/>
    <dbReference type="KEGG" id="mig:Metig_0441"/>
    <dbReference type="HOGENOM" id="CLU_113319_1_2_2"/>
    <dbReference type="OMA" id="YDWLSRI"/>
    <dbReference type="OrthoDB" id="113592at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1220.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027271">
        <property type="entry name" value="Acetolactate_synth/TF_NikR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013321">
        <property type="entry name" value="Arc_rbn_hlx_hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002145">
        <property type="entry name" value="CopG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010985">
        <property type="entry name" value="Ribbon_hlx_hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014864">
        <property type="entry name" value="TF_NikR_Ni-bd_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08753">
        <property type="entry name" value="NikR_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01402">
        <property type="entry name" value="RHH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47598">
        <property type="entry name" value="SSF47598"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="SSF55021"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="RHH_1" evidence="5">
        <location>
            <begin position="3"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="domain" description="NikR_C" evidence="6">
        <location>
            <begin position="53"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF08753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEF95997.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="131" mass="15362" checksum="6C59475BFC0B0B55" modified="2011-07-27" version="1">MERITMTIQRDLLKELEGITKELNKSRSEVIREALVDYVKKYNWLHRIESKAGDITLIYHPKVYKQILEIEKNYKDVVLVSFQSIVNDELLRVIVVKGSKERIIELTEKLKSLNDVKYAKLTTIGIPDINF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBQ7</accession>
    <name>F6BBQ7_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Sodium/hydrogen exchanger</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Metig_1655</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97187.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799778.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1655"/>
    <dbReference type="EnsemblBacteria" id="AEF97187">
        <property type="protein sequence ID" value="AEF97187"/>
        <property type="gene ID" value="Metig_1655"/>
    </dbReference>
    <dbReference type="GeneID" id="10644528"/>
    <dbReference type="KEGG" id="mig:Metig_1655"/>
    <dbReference type="HOGENOM" id="CLU_005912_9_3_2"/>
    <dbReference type="OMA" id="QIGMFVL"/>
    <dbReference type="OrthoDB" id="47913at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015299">
        <property type="term" value="F:solute:proton antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006812">
        <property type="term" value="P:cation transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006153">
        <property type="entry name" value="Cation/H_exchanger"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00999">
        <property type="entry name" value="Na_H_Exchanger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="32"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="57"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="95"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="184"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="217"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="245"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="284"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="308"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="353"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="385"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="domain" description="Na_H_Exchanger" evidence="5">
        <location>
            <begin position="13"/>
            <end position="413"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEF97187.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="419" mass="45085" checksum="EF3DD5EA76AD2EE6" modified="2011-07-27" version="1">MEYSIAIGYISLLLIAGSFIARIGEKMGLPDIPLLLLFGLFVGPVTGIISSSYAQNIFAIVGTVGLIVLLLVGAFEMRWIVLKRVLKTVLKLDTIALFISLAISGIIFNMIFKLPNLNPVGYLYGAINCATDPATLIPIFSKSNIDPEIAITLEAESVFNDPLGIVATMLGLSALGLGESTNPILEFLALAIGGGILGLVAGKIFEMVVLKEDFNEYIAPLSIGTAMAVWYVGEELIPKLIGYEFSGFMAVAIMGLYLGNVLTKYPKKSNETEKITEFCTDLSTLIRILIFVFLGASINLPLLKSYGFEGLLCAIGSIFIARPIAVFIATSVPPINESLKERLYIALEGPRGVVPAALAATVYSHIMANPSIIPVYIRQYMPPEAIAGSILVATFMTILLSVVIEGSWARPLADRLLKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBR2</accession>
    <name>F6BBR2_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">50S ribosomal protein L24e</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">rpl24e</name>
        <name evidence="6" type="ordered locus">Metig_1660</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Binds to the 23S rRNA.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="4">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Part of the 50S ribosomal subunit. Forms a cluster with proteins L3 and L14.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein eL24 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97192.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799783.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1660"/>
    <dbReference type="EnsemblBacteria" id="AEF97192">
        <property type="protein sequence ID" value="AEF97192"/>
        <property type="gene ID" value="Metig_1660"/>
    </dbReference>
    <dbReference type="GeneID" id="10644533"/>
    <dbReference type="KEGG" id="mig:Metig_1660"/>
    <dbReference type="HOGENOM" id="CLU_190191_0_0_2"/>
    <dbReference type="OMA" id="GTGKMYV"/>
    <dbReference type="OrthoDB" id="125162at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00472">
        <property type="entry name" value="Ribosomal_L24e_L24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.170.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00773">
        <property type="entry name" value="Ribosomal_L24e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038630">
        <property type="entry name" value="L24e/L24_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023438">
        <property type="entry name" value="Ribosomal_L24e"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000988">
        <property type="entry name" value="Ribosomal_L24e-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011017">
        <property type="entry name" value="TRASH_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10792">
        <property type="entry name" value="PTHR10792"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01246">
        <property type="entry name" value="Ribosomal_L24e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00746">
        <property type="entry name" value="TRASH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 6" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="4" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0699">rRNA-binding</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="TRASH" evidence="5">
        <location>
            <begin position="7"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C4-type" evidence="4">
        <location>
            <begin position="7"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="7"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="10"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="33"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="37"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEF97192.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="69" mass="8193" checksum="BA325BF6A5BEC4C6" modified="2011-07-27" version="1">MVEWKICSFCEREIEPGTGKMLVKKDGSVLYFCSSKCEKNYKMGRIPRKVKWTLTYHKLKESRKQSQSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBS2</accession>
    <name>F6BBS2_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Protein serine/threonine phosphatase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1670</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97202.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1670"/>
    <dbReference type="EnsemblBacteria" id="AEF97202">
        <property type="protein sequence ID" value="AEF97202"/>
        <property type="gene ID" value="Metig_1670"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_1670"/>
    <dbReference type="HOGENOM" id="CLU_034545_4_2_2"/>
    <dbReference type="OMA" id="MLNAQFF"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006470">
        <property type="term" value="P:protein dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00143">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015655">
        <property type="entry name" value="PP2C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036457">
        <property type="entry name" value="PPM-type_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13832">
        <property type="entry name" value="PTHR13832"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00331">
        <property type="entry name" value="PP2C_SIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00332">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81606">
        <property type="entry name" value="SSF81606"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPM-type phosphatase" evidence="1">
        <location>
            <begin position="98"/>
            <end position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF97202.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="340" mass="38612" checksum="607F5FF2CB373B2D" modified="2011-07-27" version="1">MEIIFFLINYFLNGENMDFKKIINKGLKKIFEFVESKEEGNHKIPTKLDIEEKNNIDNKYNIEIPEKPQNIEIPKIEPKLSEIKSTENINRNEIIFEENNAYGVSHKGNRENNEDYILIKKIKDIYILAVADGIGGHNAGDVASKMAVDILEEVITKNYNENLSIEEIKKLLKDAYNTAHNKIKENAIGDKEGMGTTLTTAIVKGDKCIIANCGDSRAYLIRDGEIVFRTKDHSLVQALIDEGHISEKEAMHHPMKNIITSALGLDEFKVDDYEWDLIDGDVLLMSSDGLHDYVEKETILKTVSSNNNSKDIVNELLKIALEKTKDNVSIIVYKKLTLKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBY1</accession>
    <name>F6BBY1_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glycoside hydrolase 15-related protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1729</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97261.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799850.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1729"/>
    <dbReference type="EnsemblBacteria" id="AEF97261">
        <property type="protein sequence ID" value="AEF97261"/>
        <property type="gene ID" value="Metig_1729"/>
    </dbReference>
    <dbReference type="GeneID" id="10644603"/>
    <dbReference type="KEGG" id="mig:Metig_1729"/>
    <dbReference type="HOGENOM" id="CLU_028187_0_0_2"/>
    <dbReference type="OMA" id="TWSHAMF"/>
    <dbReference type="OrthoDB" id="5867at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011613">
        <property type="entry name" value="GH15-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00723">
        <property type="entry name" value="Glyco_hydro_15"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="SSF48208"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glyco_hydro_15" evidence="1">
        <location>
            <begin position="285"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="domain" description="Glyco_hydro_15" evidence="1">
        <location>
            <begin position="343"/>
            <end position="612"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF97261.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="621" mass="72043" checksum="D0932CC414C6AAA5" modified="2011-07-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-06-02" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBZ3</accession>
    <name>F6BBZ3_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_0518</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96074.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798682.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96074">
        <property type="protein sequence ID" value="AEF96074"/>
        <property type="gene ID" value="Metig_0518"/>
    </dbReference>
    <dbReference type="GeneID" id="10643355"/>
    <dbReference type="KEGG" id="mig:Metig_0518"/>
    <dbReference type="HOGENOM" id="CLU_644987_0_0_2"/>
    <dbReference type="OMA" id="IFECKSK"/>
    <dbReference type="OrthoDB" id="104965at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF96074.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="442" mass="51070" checksum="304364C382BC186F" modified="2011-07-27" version="1">MGKESDNRTLDFHFKKYGRKGTSTEPTYKDIIEYITALISENIEDYQSQHTKLINIILNLVYPIKHFPHPLYDLGYRPKKGSTLGVKIVLDGIYYTENGNPAEITPDVILVNDNDTHVLIFECKSKSIKEEQLKKYLKLKENLHAIINKGYISVIKNPGLYKSDVSYISFDDLTTHPVLKDTEDIQILKVSPGEISLKHGSYENSKLNNIFPITWGEKEKPSYDLLICDAENNEEGSLFKMLILRELISMALFGHDEPEVGMVFSEKITLPKGEYSLESLEENNFKEIENILQKLCKRKCVIDKENKLLRVDKDIVILPEDKPLNANVFKLIDALDISINMNSLVEKEFTVGTLMDALYRSTSISIIDYFYKDYKNIVSNRIEKTLRLFSNRNLKKYLRKVSGKWRIKVIKDKRMARKFGESCKRELETIKGRQTTLDESAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-06-02" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>F6BC28</accession>
    <name>F6BC28_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">HEAT domain containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_0555</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96109.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798716.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96109">
        <property type="protein sequence ID" value="AEF96109"/>
        <property type="gene ID" value="Metig_0555"/>
    </dbReference>
    <dbReference type="GeneID" id="10643393"/>
    <dbReference type="KEGG" id="mig:Metig_0555"/>
    <dbReference type="HOGENOM" id="CLU_648317_0_0_2"/>
    <dbReference type="OMA" id="SMLLENC"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="SSF48371"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF96109.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="423" mass="49017" checksum="FF5311EEFF177C6D" modified="2011-07-27" version="1">MEYGLLNNINQLIQSNNYGERISSINLIVSKIGDKEVFKKVTPYLVKLLYDENMLVRVNALNSIKKIISEYPSIINSPEFEKLISHVYILGFTQCGIISKTLSSLINSIPKDTIKNIVTQLSYNLYSSDGLKRSLALFKISAISRYHPEYVKDILPEIMYLLTNDKLRLIQILVALMLEDFEGKETKMIFSKIPRLKYIPLDVDGFGLEVDVHPLIKIYFGSKHIGKEEIKKALEYLDDNDIIIEILSLSIIEHNVCALFELYNTKEIRDFVDKILNRLQCRYGIVRILSYLILSKIIAYKDIRNYLDEELINHCLSMLLENCEKFLMDRIFIKCYTLEAISTIYTNCENPKIKERIKEFCKKINIRDLACDHQNCYVYSILLLSSIGENVEDFITPDVYPDFVGGGKKLEKNHLLRYLGILN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>F6BC38</accession>
    <name>F6BC38_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0565</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96119.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798726.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0565"/>
    <dbReference type="EnsemblBacteria" id="AEF96119">
        <property type="protein sequence ID" value="AEF96119"/>
        <property type="gene ID" value="Metig_0565"/>
    </dbReference>
    <dbReference type="GeneID" id="10643403"/>
    <dbReference type="KEGG" id="mig:Metig_0565"/>
    <dbReference type="HOGENOM" id="CLU_067052_1_0_2"/>
    <dbReference type="OMA" id="CPAKLTP"/>
    <dbReference type="OrthoDB" id="67615at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002829">
        <property type="entry name" value="DUF116"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43801">
        <property type="entry name" value="PTHR43801"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01976">
        <property type="entry name" value="DUF116"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006594">
        <property type="entry name" value="UCP006594"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="48"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96119.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="218" mass="24822" checksum="25F90E34E390439E" modified="2011-07-27" version="1">MEILGINVLEIVGLFFILLIILLILMLLISIVVGAYLIKRNKLIFPKIFIYIADNFYSILLRLFLLIGTEETFYNVGIEFYNKYYEEQFKKSKNKVLILPHCLRDTKCPAKLTPNGVECVFCGRCCIGDIIKTAKENGYKVFIVPGSTFLKRILREVKPDGVFGVACSRDLFYGMNYLSRKGIPSQGQALIKDGCINTMVNVNELLQRLKESNANNNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>F6BC46</accession>
    <name>F6BC46_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Monosaccharide-transporting ATPase</fullName>
            <ecNumber evidence="5">3.6.3.17</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Metig_0573</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.6.3.17" evidence="5"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96127.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798734.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0573"/>
    <dbReference type="EnsemblBacteria" id="AEF96127">
        <property type="protein sequence ID" value="AEF96127"/>
        <property type="gene ID" value="Metig_0573"/>
    </dbReference>
    <dbReference type="GeneID" id="10643411"/>
    <dbReference type="KEGG" id="mig:Metig_0573"/>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_2"/>
    <dbReference type="OMA" id="EHDMRFI"/>
    <dbReference type="OrthoDB" id="42569at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="4"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="36"/>
            <end position="43"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEF96127.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="253" mass="28399" checksum="3A4A1625F6054E0C" modified="2011-07-27" version="1">MPILETKNLSKSFGGLKALNNVSIEIDESTLTLIIGPNGSGKSTFINVVSGFIPADSGRVIFDGKDITNKPPNEIYNYGIVRTFQTPQPLKEMTVLENLLIAERHIGESVLNALNYKKWLKMEEELVEKAFKILKFLKIDHLWDRKAGQLSGGQMKLLEIGRALMTNPKLIIMDEPIAGVAPGLAHEIFNHLIKLKERGITLLIVEHRLDIVLKYIDHLYVMFEGEVIAEGRGEKEIEKVLNDPKVLEVYVGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-06-02" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>F6BC53</accession>
    <name>F6BC53_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0580</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96134.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798741.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96134">
        <property type="protein sequence ID" value="AEF96134"/>
        <property type="gene ID" value="Metig_0580"/>
    </dbReference>
    <dbReference type="GeneID" id="10643418"/>
    <dbReference type="KEGG" id="mig:Metig_0580"/>
    <dbReference type="HOGENOM" id="CLU_1318547_0_0_2"/>
    <dbReference type="OMA" id="PIFWIFL"/>
    <dbReference type="OrthoDB" id="83788at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96134.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="197" mass="23152" checksum="51698B18C8A16FF8" modified="2011-07-27" version="1">MIKVYRIDGIWAILILGLILLLIIAGVFLLIPLAVLLGTYLLYRKFKVSIHEFIKKLRRKKIKIKDTSQSGEVKINFGRKIPIEDSSEISKTKEESKGEINEEIREFIEYLKNVGMEFKDGVLYYEGKKLYPTYRKSYPVNEIIKLDIPKDFDAIILGLKGFPWEPKFLYLIPIDEAKERMSIDELKKYEFKIKQIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-06-02" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>F6BC67</accession>
    <name>F6BC67_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_1741</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97273.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799862.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF97273">
        <property type="protein sequence ID" value="AEF97273"/>
        <property type="gene ID" value="Metig_1741"/>
    </dbReference>
    <dbReference type="GeneID" id="10644615"/>
    <dbReference type="KEGG" id="mig:Metig_1741"/>
    <dbReference type="HOGENOM" id="CLU_2271062_0_0_2"/>
    <dbReference type="OMA" id="CIVFYIK"/>
    <dbReference type="OrthoDB" id="104747at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF97273.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="102" mass="11890" checksum="9219E807CB3C63FA" modified="2011-07-27" version="1">MHVEYKMYDERGNPVKDKKFHCIVFYIKKSKEPSENDIMIEAVNVKNIPALVAKYMEGEVGCPGFRDPEEITNLETLKKYGVPEDIIATIKETYRKYGIDWV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCA6</accession>
    <name>F6BCA6_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Aspartate transaminase</fullName>
            <ecNumber evidence="2">2.6.1.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1780</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.6.1.1" evidence="2"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97312.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799901.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1780"/>
    <dbReference type="EnsemblBacteria" id="AEF97312">
        <property type="protein sequence ID" value="AEF97312"/>
        <property type="gene ID" value="Metig_1780"/>
    </dbReference>
    <dbReference type="GeneID" id="10644657"/>
    <dbReference type="KEGG" id="mig:Metig_1780"/>
    <dbReference type="HOGENOM" id="CLU_017584_4_2_2"/>
    <dbReference type="OMA" id="FGFECPP"/>
    <dbReference type="OrthoDB" id="32104at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004069">
        <property type="term" value="F:L-aspartate:2-oxoglutarate aminotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009058">
        <property type="term" value="P:biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotran_1_2" evidence="1">
        <location>
            <begin position="49"/>
            <end position="411"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF97312.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="432" mass="49226" checksum="88A3B55893461BFD" modified="2011-07-27" version="1">MRNNIVHVGADELTYEIREIVDIAKKIEKFGVEITWENIGDPVAKGEKIPLWIKEIVAELAMDDSSYAYCPTKGLLETREFLAELTNKRGGAQITAEDIIFFNGLGDAIAKIYGLLRKEARVIGPSPAYSTHSSAEGAHAGCPPITYYLDPENNWYPDLDDLRNKVKYNPSIAGILIINPDNPTGAVYPKKVLDEIVDIANEYDLFIISDEIYCNLVYNGKKTALLPEVIDDVCGISLKGISKEIPWPGARCGWMEVYNVDKDPIFKKYIESICRAKMVEVCSTTLPQKAIPRILSHKEYKKYLKERNAFYEKASNIAYNKLKDIEGIIVNRTNGAFYMSVVFREEYLNENQKLHVENEKLRAFVENLVKNVPYDKRFVYYLLASTGICVVPLTSFCSDLKGFRITLLEKNENKFNWIYETVSEKIKEYLNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCC2</accession>
    <name>F6BCC2_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Glycosyl transferase family 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Metig_1796</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97328.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799917.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1796"/>
    <dbReference type="EnsemblBacteria" id="AEF97328">
        <property type="protein sequence ID" value="AEF97328"/>
        <property type="gene ID" value="Metig_1796"/>
    </dbReference>
    <dbReference type="GeneID" id="10644673"/>
    <dbReference type="KEGG" id="mig:Metig_1796"/>
    <dbReference type="HOGENOM" id="CLU_023982_4_0_2"/>
    <dbReference type="OMA" id="DDILGWK"/>
    <dbReference type="OrthoDB" id="88899at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008963">
        <property type="term" value="F:phospho-N-acetylmuramoyl-pentapeptide-transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000715">
        <property type="entry name" value="Glycosyl_transferase_4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22926">
        <property type="entry name" value="PTHR22926"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00953">
        <property type="entry name" value="Glycos_transf_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="65"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="95"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="137"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="162"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="193"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="270"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEF97328.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="293" mass="32127" checksum="748236908CEEE59E" modified="2011-07-27" version="1">MEDLVIFMIVAFVLSVMLTKFIIKKMINVKYGIDLHKEEKIKVAEMGGLAPLITNIAILPFFNPYVLVVVMLSGIIGIIDDIAKLSPKEKLISLGMAAIPVGILLNMDIFHLFLLIVGVSITSNLTNMLAGFNGLEIGVGIISLIFLGLCLLIVGDVGGFQLVMVFVASYLGLFVFNKYPAKVFPGDVGTLPIGAFLATVAIWKGIILPFLIIMIPYILDASLKFYSAGVTRREEHKPTVLKNGKLYVEGGYLSLPRIILKKKPMKECEIVFVIWGISIFCGVLSLITTKILY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-09-29" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCF0</accession>
    <name>F6BCF0_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="5">DNA binding domain protein, excisionase family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Metig_0608</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96161.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798767.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96161">
        <property type="protein sequence ID" value="AEF96161"/>
        <property type="gene ID" value="Metig_0608"/>
    </dbReference>
    <dbReference type="GeneID" id="10643446"/>
    <dbReference type="KEGG" id="mig:Metig_0608"/>
    <dbReference type="HOGENOM" id="CLU_082093_0_1_2"/>
    <dbReference type="OMA" id="PQTAYRW"/>
    <dbReference type="OrthoDB" id="78802at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000150">
        <property type="term" value="F:DNA strand exchange activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03769">
        <property type="entry name" value="SR_IS607_transposase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1390">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009061">
        <property type="entry name" value="DNA-bd_dom_put_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041657">
        <property type="entry name" value="HTH_17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041718">
        <property type="entry name" value="IS607_transposase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006118">
        <property type="entry name" value="Recombinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006119">
        <property type="entry name" value="Resolv_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036162">
        <property type="entry name" value="Resolvase-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010093">
        <property type="entry name" value="SinI_DNA-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12728">
        <property type="entry name" value="HTH_17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00239">
        <property type="entry name" value="Resolvase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00857">
        <property type="entry name" value="Resolvase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46955">
        <property type="entry name" value="SSF46955"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53041">
        <property type="entry name" value="SSF53041"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01764">
        <property type="entry name" value="excise"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00397">
        <property type="entry name" value="RECOMBINASES_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51736">
        <property type="entry name" value="RECOMBINASES_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0229">DNA integration</keyword>
    <keyword evidence="3" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Resolvase/invertase-type recombinase catalytic" evidence="4">
        <location>
            <begin position="62"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEF96161.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="209" mass="24374" checksum="3C2EAFDA04B68F0E" modified="2011-07-27" version="1">MSLERLYTMKEACELLGVHIKTLQRWDREGKIKCVRTVGGKRRVPESEIKRILGIKDKEQRKIIGYARVSSNTQKDDLERQIDAIKSYAKDRGWNIEILKDVGSGLSEKRKNYRKLLKMVINREVEKVIIAYPDRLTRFGFETLKEFFKSYGTEVIVINKKCKAPQEELVEDLITVISHFAGKLYGRRSHKYKKLIKTVKEIVGENNAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCF3</accession>
    <name>F6BCF3_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Phosphoesterase</fullName>
            <ecNumber evidence="3">3.1.4.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Metig_0612</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the metallophosphoesterase superfamily. YfcE family.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.-" evidence="3"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96164.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798770.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0612"/>
    <dbReference type="EnsemblBacteria" id="AEF96164">
        <property type="protein sequence ID" value="AEF96164"/>
        <property type="gene ID" value="Metig_0612"/>
    </dbReference>
    <dbReference type="GeneID" id="10643451"/>
    <dbReference type="KEGG" id="mig:Metig_0612"/>
    <dbReference type="HOGENOM" id="CLU_063749_4_0_2"/>
    <dbReference type="OMA" id="IHCGDFV"/>
    <dbReference type="OrthoDB" id="78782at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110165">
        <property type="term" value="C:cellular anatomical entity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00841">
        <property type="entry name" value="MPP_YfcE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024654">
        <property type="entry name" value="Calcineurin-like_PHP_lpxH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041802">
        <property type="entry name" value="MPP_YfcE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020935">
        <property type="entry name" value="PdiEstase_YfcE_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000979">
        <property type="entry name" value="Phosphodiesterase_MJ0936/Vps29"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12850">
        <property type="entry name" value="Metallophos_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="SSF56300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00040">
        <property type="entry name" value="yfcE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01269">
        <property type="entry name" value="UPF0025"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metallophos_2" evidence="4">
        <location>
            <begin position="1"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU362039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF12850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEF96164.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="165" mass="18818" checksum="E44B5156500D047E" modified="2011-07-27" version="1">MKIGIMSDTHDYLPNIRKAIEIFNDEGVDLVIHCGDFVSLFVIKEFKNLKAKVIATYGNNDGERCKLKEWLKDVNEENIIDDYVSFEVDGLKFFVMHGTNKEVLDAIIASKKYDVVIYGHTHERVFNEKDGVLIINPGECCGYLTGKATIGIFDTKTKEYEEIEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCG3</accession>
    <name>F6BCG3_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="8">ATP synthase subunit c</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Metig_0624</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ATPase C chain family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96174.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798779.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0624"/>
    <dbReference type="EnsemblBacteria" id="AEF96174">
        <property type="protein sequence ID" value="AEF96174"/>
        <property type="gene ID" value="Metig_0624"/>
    </dbReference>
    <dbReference type="GeneID" id="10643463"/>
    <dbReference type="KEGG" id="mig:Metig_0624"/>
    <dbReference type="HOGENOM" id="CLU_1237912_0_0_2"/>
    <dbReference type="OMA" id="NFIQLGA"/>
    <dbReference type="OrthoDB" id="116293at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045263">
        <property type="term" value="C:proton-transporting ATP synthase complex, coupling factor F(o)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015078">
        <property type="term" value="F:proton transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015986">
        <property type="term" value="P:proton motive force-driven ATP synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.610">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000454">
        <property type="entry name" value="ATP_synth_F0_csu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038662">
        <property type="entry name" value="ATP_synth_F0_csu_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002379">
        <property type="entry name" value="ATPase_proteolipid_c-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035921">
        <property type="entry name" value="F/V-ATP_Csub_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00137">
        <property type="entry name" value="ATP-synt_C"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00124">
        <property type="entry name" value="ATPASEC"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81333">
        <property type="entry name" value="SSF81333"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="60"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="91"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="132"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="155"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="196"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="domain" description="ATP-synt_C" evidence="7">
        <location>
            <begin position="12"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="domain" description="ATP-synt_C" evidence="7">
        <location>
            <begin position="84"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="domain" description="ATP-synt_C" evidence="7">
        <location>
            <begin position="158"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEF96174.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="222" mass="21487" checksum="958034D81361D086" modified="2011-07-27" version="1">MVFENPLILGAIGAGLAVGIAGLGSGIGAGITGASGAGVVAEDPNKFGTAIVFQALPQTQGLYGFLVAILILFVFTSAPAWAMLAAGIGTGLAGLSAIGQGIASAAGLGAVAEDDSIFGKAMVFSVLPETQAIYGLLVAILLLVGVFSGPGVQEIAALGAGLAVGFAGLSGIGQGITAAGAIGATARDPDAMGKGLVLAVMPETFAIFGLLIAILIMLGIMF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCG8</accession>
    <name>F6BCG8_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 7">V-type ATP synthase beta chain</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6 7">V-ATPase subunit B</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">atpB</name>
        <name evidence="10" type="ordered locus">Metig_0629</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 7">Produces ATP from ADP in the presence of a proton gradient across the membrane. The archaeal beta chain is a regulatory subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the ATPase alpha/beta chains family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96179.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798784.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0629"/>
    <dbReference type="EnsemblBacteria" id="AEF96179">
        <property type="protein sequence ID" value="AEF96179"/>
        <property type="gene ID" value="Metig_0629"/>
    </dbReference>
    <dbReference type="GeneID" id="10643468"/>
    <dbReference type="KEGG" id="mig:Metig_0629"/>
    <dbReference type="HOGENOM" id="CLU_022916_0_0_2"/>
    <dbReference type="OMA" id="MLMMDSV"/>
    <dbReference type="OrthoDB" id="8899at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033178">
        <property type="term" value="C:proton-transporting two-sector ATPase complex, catalytic domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042777">
        <property type="term" value="P:proton motive force-driven plasma membrane ATP synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00310">
        <property type="entry name" value="ATP_synth_B_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020003">
        <property type="entry name" value="ATPase_a/bsu_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005724">
        <property type="entry name" value="ATPase_A1-cplx_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004100">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000194">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_nucl-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022879">
        <property type="entry name" value="V-ATPase_su_B/beta"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43389">
        <property type="entry name" value="PTHR43389"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00006">
        <property type="entry name" value="ATP-synt_ab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02874">
        <property type="entry name" value="ATP-synt_ab_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039114">
        <property type="entry name" value="V-ATPsynth_beta/V-ATPase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01041">
        <property type="entry name" value="ATP_syn_B_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00152">
        <property type="entry name" value="ATPASE_ALPHA_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0066">ATP synthesis</keyword>
    <keyword evidence="7" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="7" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ATP-synt_ab_N" evidence="9">
        <location>
            <begin position="13"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="domain" description="ATP-synt_ab" evidence="8">
        <location>
            <begin position="136"/>
            <end position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00031703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AEF96179.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="465" mass="51563" checksum="447E4D308B379291" modified="2011-07-27" version="1">MAAAEKTVEYTSVSRIAGPLLVVEGVKGAAYGEIVEVICPDGEKRTGQVLEARDDLAIVQVFEGTTGLSTNQTKVRFTGETAKIGVSMEMLGRIFNGAGKPIDGGPEIIPEKKLDINGYPLNPVSRKYPSDFIQTGISTIDGMNTLVRGQKLPIFSGSGLPHNQLAAQIARQAKVRGEGEQFAVVFAAMGITNEEANYFMEEFRKTGALEKAVVFINLADDPAIERILTPRIALTVAEYLAYEKDMHVLVILTDMTNYCEALREIAAARNEVPGRRGYPGYMYTDLATLYERAGRVKGRKGTITQIPILTMPHDDITHPIPDLTGYITEGQIVLSRELHRKGIYPPVDVLPSLSRLMGSGIGEGKTREDHSKVINQVYAAYAEGRSLRDLVAVVGEEALTDRDRAYLKFADEFEKRFVMQGKDEDRSIEETLDLAWELLAILPEEELKRIPDELIKKYHPKYRKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-06-02" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCH7</accession>
    <name>F6BCH7_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">UPF0056 membrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Metig_0638</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cell membrane</location>
            <topology evidence="7">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the UPF0056 (MarC) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96188.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798793.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96188">
        <property type="protein sequence ID" value="AEF96188"/>
        <property type="gene ID" value="Metig_0638"/>
    </dbReference>
    <dbReference type="GeneID" id="10643477"/>
    <dbReference type="KEGG" id="mig:Metig_0638"/>
    <dbReference type="HOGENOM" id="CLU_079909_1_0_2"/>
    <dbReference type="OMA" id="MMAIVML"/>
    <dbReference type="OrthoDB" id="88537at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002771">
        <property type="entry name" value="Multi_antbiot-R_MarC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33508">
        <property type="entry name" value="PTHR33508"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01914">
        <property type="entry name" value="MarC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00427">
        <property type="entry name" value="TIGR00427"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="6"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="45"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="111"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="147"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="181"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009784"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU362048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEF96188.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="209" mass="22724" checksum="A2731BC4C5EF3141" modified="2011-07-27" version="1">MDLNFFIYSFTSLFIIVDPIGLIPVFHLLTYYYSKDEQIEIIKSAIITATITLLIFAFFGTYIFDYFGITLDSFKVAGGLLLFKIAWDMLHAEMSKTKHSPREEVDIRMGGVAVVPLAIPLLAGPGAITTTIILMGKAQNLTDKTTVILSIISTMIISGLILSASDIVVKKLKVSGINAIVRIMGLILAAISVQIIFSGAYGLIKAITA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCM2</accession>
    <name>F6BCM2_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 10 11">Ribulose-phosphate 3-epimerase</fullName>
            <ecNumber evidence="7 10 11">5.1.3.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">rpe</name>
        <name evidence="15" type="ordered locus">Metig_0683</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the reversible epimerization of D-ribulose 5-phosphate to D-xylulose 5-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 10 11">
            <text>D-ribulose 5-phosphate = D-xylulose 5-phosphate</text>
            <dbReference type="Rhea" id="RHEA:13677"/>
            <dbReference type="ChEBI" id="CHEBI:57737"/>
            <dbReference type="ChEBI" id="CHEBI:58121"/>
            <dbReference type="EC" id="5.1.3.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10 13">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
        <text evidence="10 13">Binds 1 divalent metal cation per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="10">Carbohydrate degradation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6 10 11">Belongs to the ribulose-phosphate 3-epimerase family.</text>
    </comment>
    <dbReference type="EC" id="5.1.3.1" evidence="7 10 11"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96233.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798836.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0683"/>
    <dbReference type="EnsemblBacteria" id="AEF96233">
        <property type="protein sequence ID" value="AEF96233"/>
        <property type="gene ID" value="Metig_0683"/>
    </dbReference>
    <dbReference type="GeneID" id="10643523"/>
    <dbReference type="KEGG" id="mig:Metig_0683"/>
    <dbReference type="HOGENOM" id="CLU_054856_2_1_2"/>
    <dbReference type="OMA" id="WLQVDGG"/>
    <dbReference type="OrthoDB" id="78990at2157"/>
    <dbReference type="UniPathway" id="UPA00138"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004750">
        <property type="term" value="F:D-ribulose-phosphate 3-epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019323">
        <property type="term" value="P:pentose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006098">
        <property type="term" value="P:pentose-phosphate shunt"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00429">
        <property type="entry name" value="RPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02227">
        <property type="entry name" value="RPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026019">
        <property type="entry name" value="Ribul_P_3_epim"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000056">
        <property type="entry name" value="Ribul_P_3_epim-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11749">
        <property type="entry name" value="PTHR11749"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00834">
        <property type="entry name" value="Ribul_P_3_epim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001461">
        <property type="entry name" value="RPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="SSF51366"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01163">
        <property type="entry name" value="rpe"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01085">
        <property type="entry name" value="RIBUL_P_3_EPIMER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01086">
        <property type="entry name" value="RIBUL_P_3_EPIMER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 11" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="13" id="KW-0170">Cobalt</keyword>
    <keyword evidence="9 10 11" id="KW-0413">Isomerase</keyword>
    <keyword evidence="13" id="KW-0464">Manganese</keyword>
    <keyword evidence="8 10 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0862">Zinc</keyword>
    <feature type="region of interest" description="Substrate binding" evidence="10 14">
        <location>
            <begin position="139"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="10">
        <location>
            <begin position="172"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="10 14">
        <location>
            <begin position="194"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="10 12">
        <location>
            <position position="34"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="10 12">
        <location>
            <position position="172"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10 14">
        <location>
            <position position="7"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10 13">
        <location>
            <position position="32"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10 13">
        <location>
            <position position="34"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10 13 14">
        <location>
            <position position="65"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10 13">
        <location>
            <position position="172"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="174"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00009541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00013188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR001461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR001461-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR001461-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR001461-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="AEF96233.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="222" mass="24378" checksum="430E05A0397F7A66" modified="2011-07-27" version="1">MVMIGASILSADFGYLAEEVKKAEEAGVDFIHVDIMDGHFVPNLSIGIGIAKYVRDITKLPVDVHLMVENPEMFIEKFEGIDMITFHIETCKFPFRLINIIKNHGAKPIVALNPSTPLDTIEYILGEVYAVLVMTVEPGFSGQKFIRPMVKKIDNLKHKISKEGYDTKIFVDGGINTETASLAVNAGADALIAASAIYGKDDVRKAVEELKKAATITAVKPW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCQ5</accession>
    <name>F6BCQ5_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(R)-2-hydroxyacid dehydrogenase</fullName>
            <ecNumber evidence="3">1.1.1.272</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_0716</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LDH2/MDH2 oxidoreductase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.272" evidence="3"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96266.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798869.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0716"/>
    <dbReference type="EnsemblBacteria" id="AEF96266">
        <property type="protein sequence ID" value="AEF96266"/>
        <property type="gene ID" value="Metig_0716"/>
    </dbReference>
    <dbReference type="GeneID" id="10643556"/>
    <dbReference type="KEGG" id="mig:Metig_0716"/>
    <dbReference type="HOGENOM" id="CLU_040452_3_1_2"/>
    <dbReference type="OMA" id="TNTEPAM"/>
    <dbReference type="OrthoDB" id="32318at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050578">
        <property type="term" value="F:(R)-2-hydroxyacid dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1530.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043144">
        <property type="entry name" value="Mal/L-sulf/L-lact_DH-like_ah"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043143">
        <property type="entry name" value="Mal/L-sulf/L-lact_DH-like_NADP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036111">
        <property type="entry name" value="Mal/L-sulfo/L-lacto_DH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003767">
        <property type="entry name" value="Malate/L-lactate_DH-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11091">
        <property type="entry name" value="PTHR11091"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02615">
        <property type="entry name" value="Ldh_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89733">
        <property type="entry name" value="SSF89733"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF96266.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="347" mass="37461" checksum="48AB1104A07A02C0" modified="2011-07-27" version="1">MILLPENERKLIVDILTKYGVSEEDAKITADVFVDADLKGFTSHGIGRFPQYVEGLECGNINPNPNIKVVKETPATATIDGDFGLGQVVGKKAMELAIEKAKNVGIGAVATRNSNHFGIAGYYSEMAMREGLIGITITNTEPAMAPFGGKDKILGTNPVAIAFKGKKYVFSLDMATASIARGKILEMARLGKSIPEGCAVDKDGNLTTDPIKALEGSILPFGGPKGYGLALAIEALAAIGGAEMGTKVKGTANPKDKCTKGDLFMAIDPGAFWDREKFMEKVDELMDEIKNSEPAKGFSEVLIPGDIERMNMEKRKNGFEIDEVLYKRLKEICEKNGLDINDYVKVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCS1</accession>
    <name>F6BCS1_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0732</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SAM hydrolase / SAM-dependent halogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96282.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798885.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0732"/>
    <dbReference type="EnsemblBacteria" id="AEF96282">
        <property type="protein sequence ID" value="AEF96282"/>
        <property type="gene ID" value="Metig_0732"/>
    </dbReference>
    <dbReference type="GeneID" id="10643572"/>
    <dbReference type="KEGG" id="mig:Metig_0732"/>
    <dbReference type="HOGENOM" id="CLU_059734_1_1_2"/>
    <dbReference type="OMA" id="EGYVGAM"/>
    <dbReference type="OrthoDB" id="71537at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002747">
        <property type="entry name" value="SAM_Chlor/Fluor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023227">
        <property type="entry name" value="SAM_OH_AdoTrfase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023228">
        <property type="entry name" value="SAM_OH_AdoTrfase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35092">
        <property type="entry name" value="PTHR35092"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01887">
        <property type="entry name" value="SAM_adeno_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006779">
        <property type="entry name" value="UCP006779"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101852">
        <property type="entry name" value="SSF101852"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102522">
        <property type="entry name" value="SSF102522"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00024035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96282.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="256" mass="29069" checksum="21556DC24E6CE5C8" modified="2011-07-27" version="1">MVNPIITLTTDFGSNEGYVGAMKGKILSILREHSKSAEIIDITHEIKPFNIYHGAYVLLTFVPYFPPSIHVAVVDPTVGSERNPIVVITKNDHYLIGPDNGLFHHVVERLGIKRIIKIDEEKYAPSSTFHGRDIFAVVAAEIAVGKDIEGEELDSIVKLKTEPCIIHIDRFGNIITNIKKEDVNFEYGEKVKIKIKRNNKKEKIIECKFVKSYYEEEDEFLCLINSEGFLEISKFMDNAAELLGVDYLDEIEILNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCS7</accession>
    <name>F6BCS7_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">NIL domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0740</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96288.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798891.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0740"/>
    <dbReference type="EnsemblBacteria" id="AEF96288">
        <property type="protein sequence ID" value="AEF96288"/>
        <property type="gene ID" value="Metig_0740"/>
    </dbReference>
    <dbReference type="GeneID" id="10643579"/>
    <dbReference type="KEGG" id="mig:Metig_0740"/>
    <dbReference type="HOGENOM" id="CLU_152999_0_0_2"/>
    <dbReference type="OMA" id="VRDEDRC"/>
    <dbReference type="OrthoDB" id="111853at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018449">
        <property type="entry name" value="NIL_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09383">
        <property type="entry name" value="NIL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00930">
        <property type="entry name" value="NIL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="SSF55021"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="73"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="103"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96288.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="133" mass="14895" checksum="4FDDCAE8F25265E6" modified="2011-07-27" version="1">MKKKIILNVPPENVHKPLISKAILNTKISLNILRAKIESQKGHLVLELEGTEEQINKTLDFFSKYGEVVEIQKVIQKDEEKCIDCGSCVSLCPVDAIKMDEEFNVVFDDEKCIGCKSCIKACVVKAIKISESI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-06-02" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCT4</accession>
    <name>F6BCT4_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0747</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96295.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798898.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96295">
        <property type="protein sequence ID" value="AEF96295"/>
        <property type="gene ID" value="Metig_0747"/>
    </dbReference>
    <dbReference type="GeneID" id="10643586"/>
    <dbReference type="KEGG" id="mig:Metig_0747"/>
    <dbReference type="HOGENOM" id="CLU_2353230_0_0_2"/>
    <dbReference type="OMA" id="WIIVNTI"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="29"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96295.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="96" mass="10998" checksum="5C79726259CE1B2D" modified="2011-07-27" version="1">MDLTLFIIYFIFWIIVNTIESFRKISAIIGLFLLMGIFLKYMVFVPTLYNTGDVSLCANLITSLIYDFVQWALPPWAGFFIGSALSGNYNNIQQII</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCU1</accession>
    <name>F6BCU1_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_0755</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96302.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798905.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0755"/>
    <dbReference type="EnsemblBacteria" id="AEF96302">
        <property type="protein sequence ID" value="AEF96302"/>
        <property type="gene ID" value="Metig_0755"/>
    </dbReference>
    <dbReference type="GeneID" id="10643594"/>
    <dbReference type="KEGG" id="mig:Metig_0755"/>
    <dbReference type="HOGENOM" id="CLU_2392905_0_0_2"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF96302.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="93" mass="11114" checksum="F3D67D447F15D8A1" modified="2011-07-27" version="1">MEKKVITNKEFIDRFVKCLKNGTKFELKDYVVDGNVDILEIYKKIKEEIKDKEKLKELIEEKDDVIYVKINIEIRSSSFRVTYINDKLNIVIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCU4</accession>
    <name>F6BCU4_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Tyrosine protein kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0759</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96305.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798908.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0759"/>
    <dbReference type="EnsemblBacteria" id="AEF96305">
        <property type="protein sequence ID" value="AEF96305"/>
        <property type="gene ID" value="Metig_0759"/>
    </dbReference>
    <dbReference type="GeneID" id="10643598"/>
    <dbReference type="KEGG" id="mig:Metig_0759"/>
    <dbReference type="HOGENOM" id="CLU_095575_1_0_2"/>
    <dbReference type="OMA" id="KNFTSAM"/>
    <dbReference type="OrthoDB" id="104998at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006468">
        <property type="term" value="P:protein phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="SSF56112"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="1">
        <location>
            <begin position="28"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96305.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="217" mass="25052" checksum="1E2E93FA4891B8E4" modified="2011-07-27" version="1">MLEIKGYNEIIKNILNEEILKKLIDEGVVFKEVVGKGHRGVVLRGEFDGKDVAIKIPRKDTPKNTVLHEGKMLEIVNKVSVGPKVYRYSNDYLITEFVDGVALKYYVENLTKDDKEKLLKIVEEIFRQCVRLDLIGIDHGEIQGGKHILINDSKNKVWIIDFDKADIRFPRNFTSAISLLFGESPLSKKIKEILDVSEEEVMMLRKFAKMYKKKLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-09-29" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCW3</accession>
    <name>F6BCW3_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Class III signal peptide-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0778</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96324.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96324">
        <property type="protein sequence ID" value="AEF96324"/>
        <property type="gene ID" value="Metig_0778"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0778"/>
    <dbReference type="HOGENOM" id="CLU_1232782_0_0_2"/>
    <dbReference type="OMA" id="ITIGMEN"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007166">
        <property type="entry name" value="Class3_signal_pept_motif"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04021">
        <property type="entry name" value="Class_IIIsignal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96324.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="219" mass="24500" checksum="BA725F3C781153F6" modified="2011-07-27" version="1">MFIMFILKSFWYKNILVIIMKRGQLTLELILLLLGVVVGGTVVATQISNPNIGSGSEVKEFKNISMVAFSSPVPITTTDNNQNDVQIRSHTVYAKNININSTSSERNNELTIYDINGHIYKLYKKENRKGLIDKNDGNIIYIGEGESFSGKATKIIFRWKRGNSITLLIDGTNRTLKCKKVTLIANNIENPIKYTISHESGSGNFYLSFNATNATVIVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCY9</accession>
    <name>F6BCY9_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_0805</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96350.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96350">
        <property type="protein sequence ID" value="AEF96350"/>
        <property type="gene ID" value="Metig_0805"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0805"/>
    <dbReference type="HOGENOM" id="CLU_803184_0_0_2"/>
    <dbReference type="OMA" id="ASMMVYV"/>
    <dbReference type="OrthoDB" id="134112at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="17"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="50"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="294"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF96350.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="345" mass="40842" checksum="203AB16032DDC655" modified="2011-07-27" version="1">MNNVVDMKREFWEKLSWFFGIGSFLLIFIGLCLSLGLLKDILVNAGFDSSIGGILINAGVGFATASMMVYVALQVREIRKDRDLSLRKEHTEELRKKVVMPWIEELMTINDAKKDYSPYPKKYYPPYPKVKIGVPYKGEPFEVERIEPILFEDFLKNHATEELKNAHKKFKESSKKLYESTEKLKNNIEKFLKENRIEILESKEIYNIIKSNKINDKSRGIYFKYETLGFFLDNLLKETNEKIELSKSYRNNYPTLKFYYKDNKGNSTVYGECLCMEITEEKMRYIKNTIGNLLKEAKEKFKNDIKEIHNLIDDINNNKKIMYEELKKFEHKRIYDGNCEYIKYP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>F6BD11</accession>
    <name>F6BD11_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Phosphomethylpyrimidine kinase</fullName>
            <ecNumber evidence="2">2.7.4.7</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0827</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.7.4.7" evidence="2"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96372.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798974.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0827"/>
    <dbReference type="EnsemblBacteria" id="AEF96372">
        <property type="protein sequence ID" value="AEF96372"/>
        <property type="gene ID" value="Metig_0827"/>
    </dbReference>
    <dbReference type="GeneID" id="10643668"/>
    <dbReference type="KEGG" id="mig:Metig_0827"/>
    <dbReference type="HOGENOM" id="CLU_035788_0_0_2"/>
    <dbReference type="OMA" id="AWIEIPA"/>
    <dbReference type="OrthoDB" id="64383at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008972">
        <property type="term" value="F:phosphomethylpyrimidine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004789">
        <property type="term" value="F:thiamine-phosphate diphosphorylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009228">
        <property type="term" value="P:thiamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01169">
        <property type="entry name" value="HMPP_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.225.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036409">
        <property type="entry name" value="Aldolase_II/adducin_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045029">
        <property type="entry name" value="HMP/HMP-P_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004399">
        <property type="entry name" value="HMP/HMP-P_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013749">
        <property type="entry name" value="PM/HMP-P_kinase-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019293">
        <property type="entry name" value="ThiN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20858">
        <property type="entry name" value="PTHR20858"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08543">
        <property type="entry name" value="Phos_pyr_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10120">
        <property type="entry name" value="ThiP_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="SSF53613"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53639">
        <property type="entry name" value="SSF53639"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phos_pyr_kin" evidence="1">
        <location>
            <begin position="13"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96372.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="423" mass="47396" checksum="6F28BA5BE96F9261" modified="2011-07-27" version="1">MEKHNILIIGGYDPTGGAGVIADAKTAKVLGVNPLTITTSIIPQNNKAVYNKVDIPKKVIKEQLDAIFEDFDVSIVKTGVLNEDAINLILKYKKDYDFKIVCDPVLKSTTNYEFVDENLLEKHVDLFNECYLITPNEEEFNTIINFIEKNNLWKKFDKNPYVLVTGTNDKLITLKDKNVIETIKGKKIDKEVHGTGCVFSSAIASFLCRGEELIDAIKKAKDIVLASVVYATKTKYGYNSNPTYINKEKVIKNLSYALYLLKKINFSLIPEVGSNIAESLLLPNSFKDVAALTGRIIKNKLGGFYIVGDIEFGASEHIAKIILAAKNYDPQIRACMNIRYDEDLIDVLSEKFSISSFDRKLEPPNVSTMEWGTKYACEKFGGVPDIIYDKGGDGKEPMIRVLGVDAIDVVKKVAEIQRIYDRM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>F6BD23</accession>
    <name>F6BD23_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_0839</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96384.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798986.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0839"/>
    <dbReference type="EnsemblBacteria" id="AEF96384">
        <property type="protein sequence ID" value="AEF96384"/>
        <property type="gene ID" value="Metig_0839"/>
    </dbReference>
    <dbReference type="GeneID" id="10643680"/>
    <dbReference type="KEGG" id="mig:Metig_0839"/>
    <dbReference type="HOGENOM" id="CLU_145818_0_0_2"/>
    <dbReference type="OMA" id="YGGMKIR"/>
    <dbReference type="OrthoDB" id="107998at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016192">
        <property type="entry name" value="APOBEC/CMP_deaminase_Zn-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007154">
        <property type="entry name" value="DUF356"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04009">
        <property type="entry name" value="DUF356"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006606">
        <property type="entry name" value="UCP006606"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00903">
        <property type="entry name" value="CYT_DCMP_DEAMINASES_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF96384.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="128" mass="14807" checksum="0D11F460E4CE2A5B" modified="2011-07-27" version="1">MVLLLIRGDNYEKIKNALADVDRHAELTIIGKPRIILPEAADEILKHILGEIKKPCKKACLAKIEESAPKAIDRIRKIHPPAHIVVLSERYEMYEDLLRDFPRMPVLKGYYKSKKDSKKIKKNKHKNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>F6BD32</accession>
    <name>F6BD32_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">50S ribosomal protein L11</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">rpl11</name>
        <name evidence="8" type="ordered locus">Metig_0849</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Forms part of the ribosomal stalk which helps the ribosome interact with GTP-bound translation factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Part of the ribosomal stalk of the 50S ribosomal subunit. Interacts with L10 and the large rRNA to form the base of the stalk. L10 forms an elongated spine to which L12 dimers bind in a sequential fashion forming a multimeric L10(L12)X complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4 5">Belongs to the universal ribosomal protein uL11 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96393.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798995.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0849"/>
    <dbReference type="EnsemblBacteria" id="AEF96393">
        <property type="protein sequence ID" value="AEF96393"/>
        <property type="gene ID" value="Metig_0849"/>
    </dbReference>
    <dbReference type="GeneID" id="10643690"/>
    <dbReference type="KEGG" id="mig:Metig_0849"/>
    <dbReference type="HOGENOM" id="CLU_074237_4_0_2"/>
    <dbReference type="OMA" id="CKQFNAK"/>
    <dbReference type="OrthoDB" id="98187at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070180">
        <property type="term" value="F:large ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00349">
        <property type="entry name" value="Ribosomal_L11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.250">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00736">
        <property type="entry name" value="Ribosomal_L11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000911">
        <property type="entry name" value="Ribosomal_L11/L12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036796">
        <property type="entry name" value="Ribosomal_L11/L12_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020783">
        <property type="entry name" value="Ribosomal_L11_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036769">
        <property type="entry name" value="Ribosomal_L11_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020785">
        <property type="entry name" value="Ribosomal_L11_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020784">
        <property type="entry name" value="Ribosomal_L11_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11661">
        <property type="entry name" value="PTHR11661"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00298">
        <property type="entry name" value="Ribosomal_L11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03946">
        <property type="entry name" value="Ribosomal_L11_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00649">
        <property type="entry name" value="RL11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46906">
        <property type="entry name" value="SSF46906"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54747">
        <property type="entry name" value="SSF54747"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00359">
        <property type="entry name" value="RIBOSOMAL_L11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 5" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="4" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="Ribosomal_L11_N" evidence="7">
        <location>
            <begin position="6"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="domain" description="Ribosomal_L11" evidence="6">
        <location>
            <begin position="70"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010537"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF03946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEF96393.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="161" mass="17434" checksum="55646F033F271827" modified="2011-07-27" version="1">MAIEVVEVLVTGGKATAGPPLGPAIGPLGVNVMQVVNAINEKTKAYEGMQVPVKVKVDTEKRTFEIEVGIPPTSALIKKELGIEKGAHEPKHEVVGNLTMEQVVKIAKMKYEGMLSYTLKNAVKEVLGTCVSMGVNVEGKHPKEVTKLVDEGVYDEYFKEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>F6BD33</accession>
    <name>F6BD33_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 8">Adenylosuccinate synthetase</fullName>
            <shortName evidence="7">AMPSase</shortName>
            <shortName evidence="7">AdSS</shortName>
            <ecNumber evidence="7 8">6.3.4.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">IMP--aspartate ligase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">purA</name>
        <name evidence="9" type="ordered locus">Metig_0850</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first committed step in the biosynthesis of AMP from IMP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 8">
            <text>GTP + IMP + L-aspartate = GDP + 2 H(+) + N(6)-(1,2-dicarboxyethyl)-AMP + phosphate</text>
            <dbReference type="Rhea" id="RHEA:15753"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57567"/>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="6.3.4.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="7">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="7 8">Purine metabolism; AMP biosynthesis via de novo pathway; AMP from IMP: step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7 8">Belongs to the adenylosuccinate synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.4" evidence="7 8"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96394.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798996.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0850"/>
    <dbReference type="EnsemblBacteria" id="AEF96394">
        <property type="protein sequence ID" value="AEF96394"/>
        <property type="gene ID" value="Metig_0850"/>
    </dbReference>
    <dbReference type="GeneID" id="10643691"/>
    <dbReference type="KEGG" id="mig:Metig_0850"/>
    <dbReference type="HOGENOM" id="CLU_029848_0_0_2"/>
    <dbReference type="OMA" id="FHHAKPI"/>
    <dbReference type="OrthoDB" id="35648at2157"/>
    <dbReference type="UniPathway" id="UPA00075">
        <property type="reaction ID" value="UER00335"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004019">
        <property type="term" value="F:adenylosuccinate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044208">
        <property type="term" value="P:'de novo' AMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd03108">
        <property type="entry name" value="AdSS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.300.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.440.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.170.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00011">
        <property type="entry name" value="Adenylosucc_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018220">
        <property type="entry name" value="Adenylosuccin_syn_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042109">
        <property type="entry name" value="Adenylosuccinate_synth_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042110">
        <property type="entry name" value="Adenylosuccinate_synth_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042111">
        <property type="entry name" value="Adenylosuccinate_synth_dom3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001114">
        <property type="entry name" value="Adenylosuccinate_synthetase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11846">
        <property type="entry name" value="PTHR11846"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00709">
        <property type="entry name" value="Adenylsucc_synt"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00788">
        <property type="entry name" value="Adenylsucc_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01266">
        <property type="entry name" value="ADENYLOSUCCIN_SYN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 7 8" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1 7 8" id="KW-0436">Ligase</keyword>
    <keyword evidence="5 7 8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2 7 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 7 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4 7 8" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="IMP binding" evidence="7">
        <location>
            <begin position="13"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="region of interest" description="IMP binding" evidence="7">
        <location>
            <begin position="40"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="7">
        <location>
            <begin position="252"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="7">
        <location>
            <position position="13"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="7">
        <location>
            <position position="43"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="12"/>
            <end position="18"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="13"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="42"/>
            <end position="44"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="42"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="127"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="141"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="179"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="194"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="256"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="258"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="284"/>
            <end position="286"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="324"/>
            <end position="326"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AEF96394.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="339" mass="37129" checksum="3DAEC7A1DC48840B" modified="2011-07-27" version="1">MTCTIIVGGQWGDEGKGKIVSYLCEKDSPYIIARGGVGPNAGHTVEVDGEKYGIRMVPTGFPNKKVKLAIGAGVLVDPEVLLKEVEMLEKFDVRKRLIIDYRCGIIEEKHRLMDKSNEHLSKEIGSTGTGCGPANVDRALRILKQAKDIEALKEFLGDVSEEVNDAIDAGKNVMIEGTQGTLLSLYYGTYPYVTSKDTTASTFAADVGIGPTKVDEVIVVFKSFPTRVGCGPFPTEMPLEEAEKLGIVEYGTVTGRRRRVGYFDFELAKKVCRLNGATQIALTCLDKYDNACYGITEYGELTDKAIEFIEKIEKATGVPVTIISTGPELHQTIDRRDEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-06-02" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>F6BD34</accession>
    <name>F6BD34_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_0851</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96395.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96395">
        <property type="protein sequence ID" value="AEF96395"/>
        <property type="gene ID" value="Metig_0851"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0851"/>
    <dbReference type="HOGENOM" id="CLU_2191035_0_0_2"/>
    <dbReference type="OMA" id="RFVENWD"/>
    <dbReference type="OrthoDB" id="106607at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF96395.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="108" mass="12608" checksum="869CA1749E499AC6" modified="2011-07-27" version="1">MLGGGNIINPKKIWGELVRIAKEYFDSDREFYSKTKRGVYKIKYVSKDKVVIKKLRGKNDEVVGKKLFLNNFENLKEGKEWNCVPAVRAFLMLHPKIKEKDGVLVYEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>F6BD42</accession>
    <name>F6BD42_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">30S ribosomal protein S13</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">rps13</name>
        <name evidence="6" type="ordered locus">Metig_0859</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Located at the top of the head of the 30S subunit, it contacts several helices of the 16S rRNA. In the 70S ribosome it contacts the 23S rRNA (bridge B1a) and protein L5 of the 50S subunit (bridge B1b), connecting the 2 subunits; these bridges are implicated in subunit movement.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Part of the 30S ribosomal subunit. Forms a loose heterodimer with protein S19. Forms two bridges to the 50S subunit in the 70S ribosome.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4 5">Belongs to the universal ribosomal protein uS13 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96403.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799005.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0859"/>
    <dbReference type="EnsemblBacteria" id="AEF96403">
        <property type="protein sequence ID" value="AEF96403"/>
        <property type="gene ID" value="Metig_0859"/>
    </dbReference>
    <dbReference type="GeneID" id="10643702"/>
    <dbReference type="KEGG" id="mig:Metig_0859"/>
    <dbReference type="HOGENOM" id="CLU_103849_0_1_2"/>
    <dbReference type="OMA" id="IRAYRGI"/>
    <dbReference type="OrthoDB" id="84219at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.910.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01315">
        <property type="entry name" value="Ribosomal_S13_S18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027437">
        <property type="entry name" value="30s_Rbsml_prot_S13_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001892">
        <property type="entry name" value="Ribosomal_S13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010979">
        <property type="entry name" value="Ribosomal_S13-like_H2TH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019977">
        <property type="entry name" value="Ribosomal_S13_archaeal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018269">
        <property type="entry name" value="Ribosomal_S13_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00416">
        <property type="entry name" value="Ribosomal_S13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002134">
        <property type="entry name" value="Ribosomal_S13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46946">
        <property type="entry name" value="SSF46946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03629">
        <property type="entry name" value="uS13_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00646">
        <property type="entry name" value="RIBOSOMAL_S13_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50159">
        <property type="entry name" value="RIBOSOMAL_S13_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 5" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="4" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0699">rRNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003830"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEF96403.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="152" mass="17646" checksum="076A57B90053E1CF" modified="2011-07-27" version="1">MTQEQTDFKYLIRISRTDVDGKKPLVRALQEIYGIGEAMAKAIVRVAKLDEKKLAGYLTDEEVKKIEEILSDPAKFGIPSWMFNRRKDYHTGEDKHVIEADLTIAIQEDINRLKKIRCYRGIRHELGLPCRGQRTRSTFRRGQTVGVSRRKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>F6BD58</accession>
    <name>F6BD58_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="3">AAA ATPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_0876</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the archaeal ATPase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96419.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799021.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96419">
        <property type="protein sequence ID" value="AEF96419"/>
        <property type="gene ID" value="Metig_0876"/>
    </dbReference>
    <dbReference type="GeneID" id="10643720"/>
    <dbReference type="KEGG" id="mig:Metig_0876"/>
    <dbReference type="HOGENOM" id="CLU_068608_0_0_2"/>
    <dbReference type="OrthoDB" id="49429at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011579">
        <property type="entry name" value="ATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01637">
        <property type="entry name" value="ATPase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA" evidence="2">
        <location>
            <begin position="21"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF96419.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="364" mass="42803" checksum="A51FBFDE04490202" modified="2011-07-27" version="1">MKFFDRTEEINEILNILKKEPNDIYFIFGPINSGKTTLIKHIIENKLSNNYQVFYINFRTCLISEKREFIEAIFTTKKDTIFEKIKDKKEVLELLNKGIKIFTGVPIPEADFDNLFEKKINDAFQYLNDILLETKKSGKIPILIFDELQMIKEVVLNGQRYLLKELFQFLVSLTKEQHLCHVFCLSSDSLFIEYVYNAGELEGRAKYLLVNDFDKETALKFMNFLAVEYGIKLTNREKELIYSYVGGKAKDINYVVEEVKYKNLNEILDELLNNEILKLGIFLEKMDYIEAKINIDGNLIKVKKEDVLKALKLFKENYAVPIEKIPMHVRNFLVEENILFLNLLRRMLNPQSFLIWNAIKRVVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>F6BD76</accession>
    <name>F6BD76_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="9 10">Protein translocase subunit SecY</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Protein transport protein SEC61 subunit alpha homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">secY</name>
        <name evidence="13" type="ordered locus">Metig_0894</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9 10">The central subunit of the protein translocation channel SecYEG. Consists of two halves formed by TMs 1-5 and 6-10. These two domains form a lateral gate at the front which open onto the bilayer between TMs 2 and 7, and are clamped together by SecE at the back. The channel is closed by both a pore ring composed of hydrophobic SecY resides and a short helix (helix 2A) on the extracellular side of the membrane which forms a plug. The plug probably moves laterally to allow the channel to open. The ring and the pore may move independently.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the Sec protein translocase complex. Heterotrimer consisting of alpha (SecY), beta (SecG) and gamma (SecE) subunits. The heterotrimers can form oligomers, although 1 heterotrimer is thought to be able to translocate proteins. Interacts with the ribosome. May interact with SecDF, and other proteins may be involved.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cell membrane</location>
            <topology evidence="9">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 11">Membrane</location>
            <topology evidence="1 11">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9 12">Belongs to the SecY/SEC61-alpha family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96437.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0894"/>
    <dbReference type="EnsemblBacteria" id="AEF96437">
        <property type="protein sequence ID" value="AEF96437"/>
        <property type="gene ID" value="Metig_0894"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0894"/>
    <dbReference type="HOGENOM" id="CLU_031763_3_0_2"/>
    <dbReference type="OMA" id="KWGIGSG"/>
    <dbReference type="OrthoDB" id="13531at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0065002">
        <property type="term" value="P:intracellular protein transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006605">
        <property type="term" value="P:protein targeting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3370.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01465">
        <property type="entry name" value="SecY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026593">
        <property type="entry name" value="SecY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002208">
        <property type="entry name" value="SecY/SEC61-alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030659">
        <property type="entry name" value="SecY_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023201">
        <property type="entry name" value="SecY_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10906">
        <property type="entry name" value="PTHR10906"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00344">
        <property type="entry name" value="SecY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004557">
        <property type="entry name" value="SecY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103491">
        <property type="entry name" value="SSF103491"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00967">
        <property type="entry name" value="3a0501s007"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00755">
        <property type="entry name" value="SECY_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00756">
        <property type="entry name" value="SECY_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 9 11" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 9 11" id="KW-0811">Translocation</keyword>
    <keyword evidence="4 9 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9 11" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="33"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="71"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="114"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="142"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="173"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="213"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="251"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="320"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="377"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="401"/>
            <end position="419"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000537"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU004349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AEF96437.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="441" mass="48473" checksum="8D98C3ABA28413E4" modified="2011-07-27" version="1">MEEFLHRMKPILEKIPEVKSPDREIPFKEKLKWTGIVLILYFIMGTIDVYTGGAEIPAIFEFWQTVTASKMGTLITLGIGPIVTAGIIMQLLVGSELIRLDLSNPENRALFQGLQKLFAIVLCFVEAIMFVGAGAFGALTPAMMIILVLQLALGAILLIYLDEIVSKYGIGSGIGLFIAAGVSQTIFVGTFGPEGYLWKFFNSLVTGMPNIEYILPIIGTILVFLVVVYAESMRIEIPLAHGRIKGAVGKYPIKFIYVSNLPVILAAALFANIQLWGMFLDKMGLPILGHFVNGRPVDGLAYYLSTPYGITSVIADPLHAIIYTLAMIIFCIFFGIFWVETSGLDAKSMARRIGSLDMAIKGFRKSTKAIEQRLRRYIKPITVMSSAFVGLLAALADFTGALGGGTGVLLTVSIVYRMYEQLLQERITELHPTIAKLLNKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>F6BD79</accession>
    <name>F6BD79_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">50S ribosomal protein L30</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">rpl30</name>
        <name evidence="4" type="ordered locus">Metig_0897</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="2">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the universal ribosomal protein uL30 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96440.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799042.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0897"/>
    <dbReference type="EnsemblBacteria" id="AEF96440">
        <property type="protein sequence ID" value="AEF96440"/>
        <property type="gene ID" value="Metig_0897"/>
    </dbReference>
    <dbReference type="GeneID" id="10643741"/>
    <dbReference type="KEGG" id="mig:Metig_0897"/>
    <dbReference type="HOGENOM" id="CLU_055156_6_0_2"/>
    <dbReference type="OMA" id="KDYITWG"/>
    <dbReference type="OrthoDB" id="79065at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015934">
        <property type="term" value="C:large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01657">
        <property type="entry name" value="Ribosomal_L7_archeal_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1390.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01371_A">
        <property type="entry name" value="Ribosomal_L30_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036919">
        <property type="entry name" value="L30_ferredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005997">
        <property type="entry name" value="Ribosomal_L30_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018038">
        <property type="entry name" value="Ribosomal_L30_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016082">
        <property type="entry name" value="Ribosomal_L30_ferredoxin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039699">
        <property type="entry name" value="Ribosomal_L7/L30"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035808">
        <property type="entry name" value="Ribosomal_L7_euk_arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11524">
        <property type="entry name" value="PTHR11524"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00327">
        <property type="entry name" value="Ribosomal_L30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55129">
        <property type="entry name" value="SSF55129"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01309">
        <property type="entry name" value="uL30_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00634">
        <property type="entry name" value="RIBOSOMAL_L30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Ribosomal_L30" evidence="3">
        <location>
            <begin position="3"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00327"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEF96440.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="154" mass="17622" checksum="1095867EDBF83890" modified="2011-07-27" version="1">MAYAVVRVRGRVGVRKDIADTLKMLRLHRVNHCVIVPETDTFKGMLNKVKDYVTYGEINKDTLVKLILKRGRLPGNKRVNEEIIKEKMNMTVEELAEKLINGEIKLKDTPLKPVFRLHPPRKGYERGGIKKPFSVGGALGYRGEKINDLILRMI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>F6BD82</accession>
    <name>F6BD82_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">50S ribosomal protein L19e</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">rpl19e</name>
        <name evidence="9" type="ordered locus">Metig_0900</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Binds to the 23S rRNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5 6">Belongs to the eukaryotic ribosomal protein eL19 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96443.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799045.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0900"/>
    <dbReference type="EnsemblBacteria" id="AEF96443">
        <property type="protein sequence ID" value="AEF96443"/>
        <property type="gene ID" value="Metig_0900"/>
    </dbReference>
    <dbReference type="GeneID" id="10643744"/>
    <dbReference type="KEGG" id="mig:Metig_0900"/>
    <dbReference type="HOGENOM" id="CLU_083919_1_1_2"/>
    <dbReference type="OMA" id="NRVWIDP"/>
    <dbReference type="OrthoDB" id="109335at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070180">
        <property type="term" value="F:large ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01418">
        <property type="entry name" value="Ribosomal_L19e_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1650.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01475">
        <property type="entry name" value="Ribosomal_L19e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035970">
        <property type="entry name" value="60S_ribosomal_L19/L19e_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000196">
        <property type="entry name" value="Ribosomal_L19/L19e"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023638">
        <property type="entry name" value="Ribosomal_L19/L19e_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015972">
        <property type="entry name" value="Ribosomal_L19/L19e_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033936">
        <property type="entry name" value="Ribosomal_L19e_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039547">
        <property type="entry name" value="RPL19"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10722">
        <property type="entry name" value="PTHR10722"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01280">
        <property type="entry name" value="Ribosomal_L19e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01416">
        <property type="entry name" value="Ribosomal_L19e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48140">
        <property type="entry name" value="SSF48140"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00526">
        <property type="entry name" value="RIBOSOMAL_L19E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 5 6" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="2 5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="Ribosomal_L19e" evidence="8">
        <location>
            <begin position="2"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="52"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="54"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM01416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AEF96443.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="149" mass="17448" checksum="AA0FACE32417ECD5" modified="2011-07-27" version="1">MDVSVQRRMAADILKCGLDRVWIDPEQLDKVKAAITKDDIRALIKEGVIKKKQKKGISSARTKKLKEQKRKGRRRGPGSRRGAKGARTPKKERWINTIRPLRRLLKELRDNGTIDRRTYRRLYRMAKGGAFRSRSHMLLYMRDHDILAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDA1</accession>
    <name>F6BDA1_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Acetylornithine deacetylase or succinyl-diaminopimelate desuccinylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metig_0919</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96462.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0919"/>
    <dbReference type="EnsemblBacteria" id="AEF96462">
        <property type="protein sequence ID" value="AEF96462"/>
        <property type="gene ID" value="Metig_0919"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0919"/>
    <dbReference type="HOGENOM" id="CLU_021802_2_2_2"/>
    <dbReference type="OMA" id="STFEPTM"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010182">
        <property type="entry name" value="ArgE/DapE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036264">
        <property type="entry name" value="Bact_exopeptidase_dim_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002933">
        <property type="entry name" value="Peptidase_M20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011650">
        <property type="entry name" value="Peptidase_M20_dimer"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07687">
        <property type="entry name" value="M20_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01546">
        <property type="entry name" value="Peptidase_M20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55031">
        <property type="entry name" value="SSF55031"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01910">
        <property type="entry name" value="DapE-ArgE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="M20_dimer" evidence="3">
        <location>
            <begin position="205"/>
            <end position="315"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEF96462.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="424" mass="49010" checksum="384A3E6D807ECFAC" modified="2011-07-27" version="1">MTIRIKGVYMKDIIEETIKISSDLIKINSVNPTFGGKGEKEKAEYVLNKLGEYIKKYNIKNCTIKTYNVVDKYGIERPNIVAKFDFEKEKTLHIISHLDTVPEGDLSLWETNPYEPVIKDGMIYGRGSEDNHKAIVSSFLLLKMIFESNISPKYNLCLIFVSDEEDGSEYGLKYLLDNYEKEIFKKDDLIIVPDFGTPEGNYIEIAEKSILWIKFKIKGKQCHGSMPDDGINADILMFNFANGLYRRLYEKYNRKDGIFSPPQSTFEPTVIVNNVENTNTIPGYVEVCFDCRILPDYDVDEVLKFIEEYIKEFKKNIDKYLILYDKKNEDEIEITYEILKVEKSQRTKEDSTIVLELKDVIKKVLNKEPKLCGMGGGTVAAFLREKGYDTVVWGIGKETAHQPNEHIKIEDLIKMAKIFYEILK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDB3</accession>
    <name>F6BDB3_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Formylmethanofuran dehydrogenase subunit B</fullName>
            <ecNumber evidence="2">1.2.99.5</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0931</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.2.99.5" evidence="2"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96474.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0931"/>
    <dbReference type="KEGG" id="mig:Metig_0931"/>
    <dbReference type="HOGENOM" id="CLU_034348_0_0_2"/>
    <dbReference type="OMA" id="VCIDPHE"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018493">
        <property type="term" value="F:formylmethanofuran dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015948">
        <property type="term" value="P:methanogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02761">
        <property type="entry name" value="MopB_FmdB-FwdB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016457">
        <property type="entry name" value="Formylmethanofuran_DH_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006656">
        <property type="entry name" value="Mopterin_OxRdtase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00384">
        <property type="entry name" value="Molybdopterin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005646">
        <property type="entry name" value="FwdB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03129">
        <property type="entry name" value="one_C_dehyd_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0711">Selenium</keyword>
    <keyword evidence="2" id="KW-0712">Selenocysteine</keyword>
    <feature type="domain" description="Molybdopterin" evidence="1">
        <location>
            <begin position="51"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="non-standard amino acid" description="Selenocysteine" evidence="2">
        <location>
            <position position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96474.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="435" mass="48778" checksum="525CD749AAB73881" modified="2011-07-27" version="1">MVEVVKNVVCPFCGTLCDDLEILVEDGHIVGTRHACRIGNAKFMHFEGAVRYTEPLMRENKKDEFKKVDYETAIEETARLLVEAKLPLIYGFSATECHAHQYAVKLAEKVSAIIDNTASVUHGPSILGLQDVGYPVCTCGEVKNRADVIIYWASNPMHAHPRHMSRYGVFPRGFFRERGRKDRTLIVVDPRKTDTAKLADIHLQVEQHKDYELISAMRAALKGFELQVDEVAGIPVELIYEAVDICKNAQFGQLFFGMGVTMTKGKHRNIDNAIQLIIDLNAYTKFGLMPMRGHYNVNGFNQVLTWVTGYPFGVDFSRGYPRFNPGETCANDVLQRGETDMMLNIASDPGAHFPQKAVQHMARIPLVCIDPHETPTTHLANIIIPPAIAGVEVEGTAYRMDGVPIELKKVVEPPEGLLPDREILKMLIKKVDEMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDD3</accession>
    <name>F6BDD3_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Acetate--CoA ligase (ADP-forming)</fullName>
            <ecNumber evidence="2">6.2.1.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Metig_0951</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>acetate + ATP + CoA = acetyl-CoA + ADP + phosphate</text>
            <dbReference type="Rhea" id="RHEA:15081"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.2.1.13"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="6.2.1.13" evidence="2"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96494.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799096.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0951"/>
    <dbReference type="EnsemblBacteria" id="AEF96494">
        <property type="protein sequence ID" value="AEF96494"/>
        <property type="gene ID" value="Metig_0951"/>
    </dbReference>
    <dbReference type="GeneID" id="10643797"/>
    <dbReference type="KEGG" id="mig:Metig_0951"/>
    <dbReference type="HOGENOM" id="CLU_007415_3_1_2"/>
    <dbReference type="OMA" id="SFMSQSG"/>
    <dbReference type="OrthoDB" id="2789at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043758">
        <property type="term" value="F:acetate-CoA ligase (ADP-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.261">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014089">
        <property type="entry name" value="AcCoA-synth-alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003781">
        <property type="entry name" value="CoA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043938">
        <property type="entry name" value="Ligase_CoA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032875">
        <property type="entry name" value="Succ_CoA_lig_flav_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016102">
        <property type="entry name" value="Succinyl-CoA_synth-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13380">
        <property type="entry name" value="CoA_binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19045">
        <property type="entry name" value="Ligase_CoA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13607">
        <property type="entry name" value="Succ_CoA_lig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00881">
        <property type="entry name" value="CoA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52210">
        <property type="entry name" value="SSF52210"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02717">
        <property type="entry name" value="AcCoA-syn-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-grasp" evidence="5">
        <location>
            <begin position="492"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="454"/>
            <end position="481"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEF96494.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="701" mass="77704" checksum="19B2A8203205BC93" modified="2011-07-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-06-02" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDF4</accession>
    <name>F6BDF4_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_0972</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96515.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799117.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96515">
        <property type="protein sequence ID" value="AEF96515"/>
        <property type="gene ID" value="Metig_0972"/>
    </dbReference>
    <dbReference type="GeneID" id="55575284"/>
    <dbReference type="KEGG" id="mig:Metig_0972"/>
    <dbReference type="HOGENOM" id="CLU_978658_0_0_2"/>
    <dbReference type="OMA" id="WIPPYTT"/>
    <dbReference type="OrthoDB" id="56925at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF96515.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="264" mass="29747" checksum="B72A0C2795CDEED2" modified="2011-07-27" version="1">MRFLLLILLLTFLNLSFAGQIATMQIDYKLTGEITNLNPYSIFVAIPDDITYTEKTLPTSGDVTKVSVETINESELITFGTSILNGKIGYWIPPYTTVKITIYGVQPEIYALNVDDSQKGYDIVGPAVVNSIKVINLKENFPLSKRKGIKISNFKLYVWGSIIKDDDAGVLSIVLPTPLILDNYNRFYKIVGKNDPDVWISSYNKWFKEHVKKTDDVNLDMDDPLIPRMDYDVGVDIEVLDVPAIAFTTSSTQPIEFSYVMYGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDH1</accession>
    <name>F6BDH1_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">50S ribosomal protein L44e</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">rpl44e</name>
        <name evidence="4" type="ordered locus">Metig_0990</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Binds to the 23S rRNA.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="2">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 2 3">Belongs to the eukaryotic ribosomal protein eL42 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96532.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799134.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0990"/>
    <dbReference type="EnsemblBacteria" id="AEF96532">
        <property type="protein sequence ID" value="AEF96532"/>
        <property type="gene ID" value="Metig_0990"/>
    </dbReference>
    <dbReference type="GeneID" id="10643837"/>
    <dbReference type="KEGG" id="mig:Metig_0990"/>
    <dbReference type="HOGENOM" id="CLU_114645_3_0_2"/>
    <dbReference type="OMA" id="CKKHTIH"/>
    <dbReference type="OrthoDB" id="106603at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070180">
        <property type="term" value="F:large ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01476">
        <property type="entry name" value="Ribosomal_L44e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000552">
        <property type="entry name" value="Ribosomal_L44e"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011332">
        <property type="entry name" value="Ribosomal_zn-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10369">
        <property type="entry name" value="PTHR10369"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00935">
        <property type="entry name" value="Ribosomal_L44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57829">
        <property type="entry name" value="SSF57829"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01172">
        <property type="entry name" value="RIBOSOMAL_L44E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 3 4" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="2" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2" id="KW-0699">rRNA-binding</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="binding site" evidence="2">
        <location>
            <position position="11"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="14"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="70"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="73"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU000666"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEF96532.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="93" mass="11114" checksum="612DEAE9F9D9A486" modified="2011-07-27" version="1">MKMKKRIRRYCPYCKKHTEHIVERAKKRKASELTWGQRQFRRVLRGYGGYPRPLPDNAKPTKKLDLRYKCTVCGKMHTRANGGFRVSKFELVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDH5</accession>
    <name>F6BDH5_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0994</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96536.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0994"/>
    <dbReference type="EnsemblBacteria" id="AEF96536">
        <property type="protein sequence ID" value="AEF96536"/>
        <property type="gene ID" value="Metig_0994"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0994"/>
    <dbReference type="HOGENOM" id="CLU_893144_0_0_2"/>
    <dbReference type="OMA" id="SPKIINC"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96536.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="344" mass="39098" checksum="6102B94E0C05AD04" modified="2011-07-27" version="1">MNVTLILLYSLLEKLIYKLMVIIMIIPHRTPCLEGKINVEGEEEHLKLVLSHLLKKRKNEDNIKILPSGNAAIFIASKIVKDILGEGNILIPDMGGWRGFLRYPKMLNLNPIKIKTNLGVVDIEELISSIEKYNPKALFLTSLAGYLAPQPLKEIKKVCEEFGILFVEDVSGKIGDDCGYGDIVVCSMGSPKIINCEYGGFLAFDEEIKEELKKVNKLDELNALIKTYKVPNFFGLMKEEAKNAKKVYKKCVYFSNIIKSEMENAYFKDKDGVCVFLEYENPREVAKFVDSRVKLDNGKSIITKCPLYDRILRNGIVVEIKKMDVLNIDKDNIIEIVNILKKQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDI5</accession>
    <name>F6BDI5_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">UPF0146 protein Metig_1004</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_1004</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the UPF0146 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96546.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799148.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1004"/>
    <dbReference type="EnsemblBacteria" id="AEF96546">
        <property type="protein sequence ID" value="AEF96546"/>
        <property type="gene ID" value="Metig_1004"/>
    </dbReference>
    <dbReference type="GeneID" id="10643852"/>
    <dbReference type="KEGG" id="mig:Metig_1004"/>
    <dbReference type="HOGENOM" id="CLU_148458_0_0_2"/>
    <dbReference type="OMA" id="LIYSIRP"/>
    <dbReference type="OrthoDB" id="92832at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00341">
        <property type="entry name" value="UPF0146"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005353">
        <property type="entry name" value="UPF0146"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03686">
        <property type="entry name" value="UPF0146"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016725">
        <property type="entry name" value="UCP016725"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00341"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF96546.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="133" mass="15421" checksum="0A33694A593A6016" modified="2011-07-27" version="1">MNENVIFEFIFNYAKNNNYKKIVEIGVGFNINIAKKLHEFFDVCVVDINRNAIENAKKEGLNGFVDNVFSPNLSIYTNTDLIYSIRPPRDLQLFILDIAKKINADLIIRPLSNEFPVDLKLKNYNGEIFYVLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDL1</accession>
    <name>F6BDL1_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DNA-directed RNA polymerase subunit Rpo7</fullName>
            <ecNumber evidence="5">2.7.7.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">DNA-directed RNA polymerase subunit E</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">rpo7</name>
        <name evidence="5" type="synonym">rpoE</name>
        <name evidence="7" type="ordered locus">Metig_1030</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">DNA-dependent RNA polymerase (RNAP) catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + RNA(n+1)</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="5">Part of the RNA polymerase complex. Forms a stalk with Rpo4 that extends from the main structure.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="5">Forms 2 domains with an elongated structure; Rpo4 packs into the hinge region between the 2 domains.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the eukaryotic RPB7/RPC8 RNA polymerase subunit family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="5"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96572.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799174.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1030"/>
    <dbReference type="EnsemblBacteria" id="AEF96572">
        <property type="protein sequence ID" value="AEF96572"/>
        <property type="gene ID" value="Metig_1030"/>
    </dbReference>
    <dbReference type="GeneID" id="10643884"/>
    <dbReference type="KEGG" id="mig:Metig_1030"/>
    <dbReference type="HOGENOM" id="CLU_117966_0_0_2"/>
    <dbReference type="OMA" id="MRQPGLG"/>
    <dbReference type="OrthoDB" id="117241at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed 5'-3' RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription, initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00865">
        <property type="entry name" value="RNApol_arch_Rpo7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036898">
        <property type="entry name" value="RNA_pol_Rpb7-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004519">
        <property type="entry name" value="RNAP_E/RPC8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046399">
        <property type="entry name" value="RNApol_Rpo7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045113">
        <property type="entry name" value="Rpb7-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005576">
        <property type="entry name" value="Rpb7-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022967">
        <property type="entry name" value="S1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003029">
        <property type="entry name" value="S1_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12709">
        <property type="entry name" value="PTHR12709"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00575">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03876">
        <property type="entry name" value="SHS2_Rpb7-N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00316">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88798">
        <property type="entry name" value="SSF88798"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00448">
        <property type="entry name" value="rpoE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50126">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 5 7" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="5 7" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0804">Transcription</keyword>
    <keyword evidence="5 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="S1 motif" evidence="6">
        <location>
            <begin position="82"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEF96572.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="188" mass="21294" checksum="34BD4D5651D2BB1D" modified="2011-07-27" version="1">MYKILTISDVIRVPPNMFGRPLKETITNILREKYEGILDRDIGFILSIVDVKEIGEGKIIHGDGAAYHPVVFDALIYIPELHEVVEGEIVDIVEFGAFVRLGPLDGLIHISQIMDDYVTYDPKREAIIGKETGKVLEIGDRVRARIVAISLREERKRGSKIALTMRQPGLGKLEWIEEEKKKSQQSNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDL4</accession>
    <name>F6BDL4_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">7-carboxy-7-deazaguanine synthase</fullName>
            <shortName evidence="5">CDG synthase</shortName>
            <ecNumber evidence="5">4.3.99.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Archaeosine biosynthesis protein QueE</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">queE</name>
        <name evidence="7" type="ordered locus">Metig_1033</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the complex heterocyclic radical-mediated conversion of 6-carboxy-5,6,7,8-tetrahydropterin (CPH4) to 7-carboxy-7-deazaguanine (CDG), a step common to the biosynthetic pathways of all 7-deazapurine-containing compounds.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>6-carboxy-5,6,7,8-tetrahydropterin + H(+) = 7-carboxy-7-deazaguanine + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:27974"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:61032"/>
            <dbReference type="ChEBI" id="CHEBI:61036"/>
            <dbReference type="EC" id="4.3.99.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </cofactor>
        <text evidence="5">Binds 1 S-adenosyl-L-methionine per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="5">Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="pathway">
        <text evidence="5">Purine metabolism; 7-cyano-7-deazaguanine biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the radical SAM superfamily. 7-carboxy-7-deazaguanine synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="4.3.99.3" evidence="5"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96575.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799177.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1033"/>
    <dbReference type="EnsemblBacteria" id="AEF96575">
        <property type="protein sequence ID" value="AEF96575"/>
        <property type="gene ID" value="Metig_1033"/>
    </dbReference>
    <dbReference type="GeneID" id="10643887"/>
    <dbReference type="KEGG" id="mig:Metig_1033"/>
    <dbReference type="HOGENOM" id="CLU_066739_1_0_2"/>
    <dbReference type="OMA" id="IPQTHKM"/>
    <dbReference type="OrthoDB" id="64973at2157"/>
    <dbReference type="UniPathway" id="UPA00391"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016840">
        <property type="term" value="F:carbon-nitrogen lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904047">
        <property type="term" value="F:S-adenosyl-L-methionine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00917">
        <property type="entry name" value="QueE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024924">
        <property type="entry name" value="7-CO-7-deazaguanine_synth-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="3 5" id="KW-0408">Iron</keyword>
    <keyword evidence="4 5" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="5" id="KW-0456">Lyase</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="6">
        <location>
            <begin position="15"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="5">
        <location>
            <begin position="9"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="region of interest" description="S-adenosyl-L-methionine binding" evidence="5">
        <location>
            <begin position="34"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="24"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="28"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="32"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="35"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="86"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="88"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEF96575.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="244" mass="28195" checksum="C4A3CE35ECF6BBD3" modified="2011-07-27" version="1">MIREIFSSIMGEGKFVGRRFIFIRFKKCPLNCIYCDEVSKGEGECRVEVIPGTRNFKIYNEEEIEDKLVEIVNSLKTPDLFAVSFTGGEPLVYAEYLKKYSKILKEEGYKTYLESNGMYPGRVSFFDYASIDIKLPEHFEDIKEEDYKKLYKNELKTIEKLYNMGSDVFAKVIIMENTSVEFVENIAKDLSDIGNIMLCIQPVTPYGKVRRMPTQKKIFDIMSACGKHVDVMCTPQIHKLLGML</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDL9</accession>
    <name>F6BDL9_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Coenzyme F420 hydrogenase</fullName>
            <ecNumber evidence="4">1.12.98.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metig_1038</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.12.98.1" evidence="4"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96580.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1038"/>
    <dbReference type="EnsemblBacteria" id="AEF96580">
        <property type="protein sequence ID" value="AEF96580"/>
        <property type="gene ID" value="Metig_1038"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_1038"/>
    <dbReference type="HOGENOM" id="CLU_037958_0_0_2"/>
    <dbReference type="OMA" id="AGSRYNW"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050454">
        <property type="term" value="F:coenzyme F420 hydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007516">
        <property type="entry name" value="Co_F420_Hydgase/DH_bsu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045220">
        <property type="entry name" value="FRHB/FDHB/HCAR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007525">
        <property type="entry name" value="FrhB_FdhB_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31332">
        <property type="entry name" value="PTHR31332"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00037">
        <property type="entry name" value="Fer4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04432">
        <property type="entry name" value="FrhB_FdhB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04422">
        <property type="entry name" value="FrhB_FdhB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0408">Iron</keyword>
    <keyword evidence="2" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="3">
        <location>
            <begin position="18"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEF96580.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="366" mass="40704" checksum="1537FB63986FEDEA" modified="2011-07-27" version="1">MKKHRLKVKIMKSYLKLKEEVWDKNICSGCGACVAVCPVENIYFKQQSPVKFKCELCACTIDPAAETECPASAEFCKVTLYNVPCGACYDACPRTEEKLEIPIEEIGKYIEIFGAKSKMDIKYAQSGGAVTAILCNALDEGLIDGAIVVSEDKWTMEPKSVLATTKEELIKAAGSRYNWNVPILEALKEAVMVRKLEKLAIVGTPCVINAVFQILASDNDLLRPFKKAIRLKIGLFCTETFKYSELMAKIKEMGINPWEIKKMEIKKGKLAIDLLNGETKEIPLKEIEYCVRKGCSVCRDFTALVSDISAGNVGTPEGVTTLIVRNEWGKGFVERAILNGYLEKVDCEVNIDAIKKLAKKKMERGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDM6</accession>
    <name>F6BDM6_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein-L-isoaspartate O-methyltransferase</fullName>
            <ecNumber evidence="4">2.1.1.77</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">L-isoaspartyl protein carboxyl methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">Protein L-isoaspartyl methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">Protein-beta-aspartate methyltransferase</fullName>
            <shortName evidence="4">PIMT</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">pcm</name>
        <name evidence="5" type="ordered locus">Metig_1046</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 4">Catalyzes the methyl esterification of L-isoaspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3 4">
            <text>[protein]-L-isoaspartate + S-adenosyl-L-methionine = [protein]-L-isoaspartate alpha-methyl ester + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:12705"/>
            <dbReference type="Rhea" id="RHEA-COMP:12143"/>
            <dbReference type="Rhea" id="RHEA-COMP:12144"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:90596"/>
            <dbReference type="ChEBI" id="CHEBI:90598"/>
            <dbReference type="EC" id="2.1.1.77"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the methyltransferase superfamily. L-isoaspartyl/D-aspartyl protein methyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.77" evidence="4"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96587.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1046"/>
    <dbReference type="EnsemblBacteria" id="AEF96587">
        <property type="protein sequence ID" value="AEF96587"/>
        <property type="gene ID" value="Metig_1046"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_1046"/>
    <dbReference type="HOGENOM" id="CLU_055432_2_0_2"/>
    <dbReference type="OMA" id="TISAIHM"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004719">
        <property type="term" value="F:protein-L-isoaspartate (D-aspartate) O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030091">
        <property type="term" value="P:protein repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00090">
        <property type="entry name" value="PIMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000682">
        <property type="entry name" value="PCMT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11579">
        <property type="entry name" value="PTHR11579"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00080">
        <property type="entry name" value="pimt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01279">
        <property type="entry name" value="PCMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 5" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4 5" id="KW-0808">Transferase</keyword>
    <feature type="active site" evidence="4">
        <location>
            <position position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00025330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00029295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEF96587.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="214" mass="23934" checksum="FAB50223CC3F2B85" modified="2011-07-27" version="1">MIEKMKPIVERLMYEGYIKNKKVAEALLKVPRHEFVPEDLKEYAYIDSPLEIGFGQTISAIHMVAMMCDILDLKEGQKVLEVGTGCGYHAAVTAELVGKDGLVVTVERFPELAKRAEKTLRRLGYDNVIVICGDGTLGYEPLAPYDRIYVTAAGPKVPRPLIEQLKDGGKLLIPVGRYVQHLILIEKKDGKIIEKDFGEVSFVPLVGEEGWKDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDQ5</accession>
    <name>F6BDQ5_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="5">DNA-cytosine methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Metig_1077</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3 4">Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96616.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799217.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1077"/>
    <dbReference type="REBASE" id="36259">
        <property type="entry name" value="M.Mig5ORF1077P"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96616">
        <property type="protein sequence ID" value="AEF96616"/>
        <property type="gene ID" value="Metig_1077"/>
    </dbReference>
    <dbReference type="GeneID" id="10643931"/>
    <dbReference type="KEGG" id="mig:Metig_1077"/>
    <dbReference type="HOGENOM" id="CLU_006958_2_2_2"/>
    <dbReference type="OMA" id="EPFTGAN"/>
    <dbReference type="OrthoDB" id="58937at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001525">
        <property type="entry name" value="C5_MeTfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00145">
        <property type="entry name" value="DNA_methylase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00105">
        <property type="entry name" value="C5METTRFRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00675">
        <property type="entry name" value="dcm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51679">
        <property type="entry name" value="SAM_MT_C5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3 5" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2 3 5" id="KW-0808">Transferase</keyword>
    <feature type="active site" evidence="3">
        <location>
            <position position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEF96616.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="358" mass="40913" checksum="1D5BB07AC772AB76" modified="2011-07-27" version="1">MRFKFVDLFCGCGGFSRGFVEEGFEPVVAIEIEERAASAYALNFNGRLYEKRLNELVEKGISCYVGDILFNIPLTDKDVEILKKLGTYRGDINPVVINDDIREIHSYDILKICKKVDVVIGGPPCEAYTGANPNREKNPFDRLYKDEMGRLVLEYIRIVGDLQPEIFVMENVPGILEPEIKESIEKEFKKVGYDVYFNVLRAEEYGNPSSRRRVFVSNIEINPEKSEPVVVIKAIEDLMFKGRDVPNHEYVTLPRRVLKRLPKAKWGEGVVKFKGSCGVLDNYIKLHPYKIAPTVMGKRRFIHPFENRSLTPREQARLMGYPDYHLFVGGKDSQFNQIGESVPPTLSRAIAKVVKENL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDS0</accession>
    <name>F6BDS0_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Pyridoxal phosphate enzyme</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_1092</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96631.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799232.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1092"/>
    <dbReference type="EnsemblBacteria" id="AEF96631">
        <property type="protein sequence ID" value="AEF96631"/>
        <property type="gene ID" value="Metig_1092"/>
    </dbReference>
    <dbReference type="GeneID" id="10643947"/>
    <dbReference type="KEGG" id="mig:Metig_1092"/>
    <dbReference type="HOGENOM" id="CLU_055443_0_0_2"/>
    <dbReference type="OMA" id="RIDFCSK"/>
    <dbReference type="OrthoDB" id="37272at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009058">
        <property type="term" value="P:biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020033">
        <property type="entry name" value="PyrdxlP-dep_transferase_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03576">
        <property type="entry name" value="pyridox_MJ0158"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminotran_1_2" evidence="2">
        <location>
            <begin position="78"/>
            <end position="346"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF96631.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="365" mass="40545" checksum="22E4F46F92296FD3" modified="2011-07-27" version="1">MDDDNELLRLEKSRKLIREIIKKKGRNGIYDLTGLAGGFKITEEDKALLETYVGPAVYSEELNKLGLIHLRGNSKNHKVVGFNRTSSAILATILTIKPKKVVHYVPELPSHPSIPKSCKIVGAEYFEYDNVEETLKKIDEYTLTIITGATMDHKVINLDAMKEIINYAKKKNSIVFVDDASGARLRLLFNQPSALELGADLVVTSTDKLMDGPRGGLLGGLKELVNKIYTVGLSFGLEAQPPLMAGMVRGLESFNLQKLKRAFDRAKNIDLSKLDELNIQYEKTPTGFVIKKLDKNKDAHENEKDLIKVAMALLEDYGIVTITSVGMPGASKSIRIDLCSKDAERLSDEEIVNAIINSIKKVMKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-06-02" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDS8</accession>
    <name>F6BDS8_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1100</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96639.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799240.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96639">
        <property type="protein sequence ID" value="AEF96639"/>
        <property type="gene ID" value="Metig_1100"/>
    </dbReference>
    <dbReference type="GeneID" id="10643958"/>
    <dbReference type="KEGG" id="mig:Metig_1100"/>
    <dbReference type="HOGENOM" id="CLU_1405987_0_0_2"/>
    <dbReference type="OMA" id="WHYITST"/>
    <dbReference type="OrthoDB" id="95394at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="61"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96639.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="191" mass="22723" checksum="2A61CA7D137353E4" modified="2011-07-27" version="1">MDIGLILFIIILFLFPYLIVLMIVFNPYGILDYLLYKKYKKAKGEWHYITSTKIGMNRSRWIFILMVNIITLFFGLYILININHPDDEILTFSLIFLSIAIIYDKLNPTSGTVEIYKEGIAIYIKRFNILKPFLNYYLVLPWKFFKGYKVKSKNKTKYVILIPKSKLFFNIYLKDDGKVEEIIKNYLNPVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDT7</accession>
    <name>F6BDT7_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Acylphosphatase</fullName>
            <ecNumber evidence="1">3.6.1.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Metig_1109</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>an acyl phosphate + H2O = a carboxylate + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:14965"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29067"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:59918"/>
            <dbReference type="EC" id="3.6.1.7"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the acylphosphatase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.7" evidence="1"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96648.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799249.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96648">
        <property type="protein sequence ID" value="AEF96648"/>
        <property type="gene ID" value="Metig_1109"/>
    </dbReference>
    <dbReference type="GeneID" id="10643967"/>
    <dbReference type="KEGG" id="mig:Metig_1109"/>
    <dbReference type="HOGENOM" id="CLU_1656874_0_0_2"/>
    <dbReference type="OMA" id="ETREEPY"/>
    <dbReference type="OrthoDB" id="71068at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003998">
        <property type="term" value="F:acylphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020456">
        <property type="entry name" value="Acylphosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001792">
        <property type="entry name" value="Acylphosphatase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036046">
        <property type="entry name" value="Acylphosphatase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017968">
        <property type="entry name" value="Acylphosphatase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47268">
        <property type="entry name" value="PTHR47268"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00708">
        <property type="entry name" value="Acylphosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54975">
        <property type="entry name" value="SSF54975"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00150">
        <property type="entry name" value="ACYLPHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51160">
        <property type="entry name" value="ACYLPHOSPHATASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acylphosphatase-like" evidence="4">
        <location>
            <begin position="4"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="121"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="19"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU004168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEF96648.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="189" mass="22020" checksum="1524E37A50D8070C" modified="2011-07-27" version="1">MSTTYELIIYGKVQHVGFRDRIENIGKGLGIDGIVYNFKDGTVRILANFEDEDIKEFFKKSIKMLEKKDNLIKIDKIEEKELNAFIEFPEGINRISADDLLELNKKLDEGVKYIKLIFGSLEELNVKVDKQTEILEKLNVKMDKQTEILEKLNAKMDKQTEILTELKKGHDEVRETLNKMVEILEKILK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDU6</accession>
    <name>F6BDU6_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Argininosuccinate lyase</fullName>
            <shortName evidence="1">ASAL</shortName>
            <ecNumber evidence="1">4.3.2.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Arginosuccinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">argH</name>
        <name evidence="5" type="ordered locus">Metig_1118</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2-(N(omega)-L-arginino)succinate = fumarate + L-arginine</text>
            <dbReference type="Rhea" id="RHEA:24020"/>
            <dbReference type="ChEBI" id="CHEBI:29806"/>
            <dbReference type="ChEBI" id="CHEBI:32682"/>
            <dbReference type="ChEBI" id="CHEBI:57472"/>
            <dbReference type="EC" id="4.3.2.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 3/3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the lyase 1 family. Argininosuccinate lyase subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.3.2.1" evidence="1"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96657.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799258.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1118"/>
    <dbReference type="EnsemblBacteria" id="AEF96657">
        <property type="protein sequence ID" value="AEF96657"/>
        <property type="gene ID" value="Metig_1118"/>
    </dbReference>
    <dbReference type="GeneID" id="10643976"/>
    <dbReference type="KEGG" id="mig:Metig_1118"/>
    <dbReference type="HOGENOM" id="CLU_027272_2_3_2"/>
    <dbReference type="OMA" id="KKNPDVF"/>
    <dbReference type="OrthoDB" id="51806at2157"/>
    <dbReference type="UniPathway" id="UPA00068">
        <property type="reaction ID" value="UER00114"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004056">
        <property type="term" value="F:argininosuccinate lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042450">
        <property type="term" value="P:arginine biosynthetic process via ornithine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01359">
        <property type="entry name" value="Argininosuccinate_lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.275.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00006">
        <property type="entry name" value="Arg_succ_lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029419">
        <property type="entry name" value="Arg_succ_lyase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009049">
        <property type="entry name" value="Argininosuccinate_lyase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024083">
        <property type="entry name" value="Fumarase/histidase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000362">
        <property type="entry name" value="Fumarate_lyase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022761">
        <property type="entry name" value="Fumarate_lyase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008948">
        <property type="entry name" value="L-Aspartase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43814">
        <property type="entry name" value="PTHR43814"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14698">
        <property type="entry name" value="ASL_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00206">
        <property type="entry name" value="Lyase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00149">
        <property type="entry name" value="FUMRATELYASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48557">
        <property type="entry name" value="SSF48557"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00838">
        <property type="entry name" value="argH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="1" id="KW-0055">Arginine biosynthesis</keyword>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1 5" id="KW-0456">Lyase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Lyase_1" evidence="3">
        <location>
            <begin position="17"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="domain" description="ASL_C2" evidence="4">
        <location>
            <begin position="364"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="451"/>
            <end position="485"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF14698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEF96657.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="485" mass="55261" checksum="1E26F50FA66D996B" modified="2011-07-27" version="1">MNILRRGRLGNSVKNDVAKYTTSLDFDREIFEADILCDIAHVIMLMENNIISKEDAKKIIEGLKEIYKKGMENLNLDPSLDDIHMVIESELIKKLGEDVAGRMHTGRSRNDEVATDLRMALREKVLLILKMLINLEKDLLNMAKEHKETLMVGYTHLQHAQPTTFAHHLLSYVSAIERDILRLLDTYKRINISPLGSGAMATTGFDIDRERTKELLGFDGIIENSMDAVSARDFIAETMANLSILGTNLSKICEELILFSTYEFGIVEIANEYTSTSSIMPQKKNPDVAEIARAKLSTLNGNLISVLTILKALPNTYNRDLQEISPHLWKSVYTTIDTIKMIHGMLKTLKVNADRMRELANANYSTATELADTLVRECNIPFRTAHGIVGEVVRRAIEEKKDMIKVIYEVLEKYNLKIDEEKIKKALDPYENVKMRDVVGGPAPKEVERAIKSFKERINKYEKEVDEKIEKINKVKENLLSYEIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-06-02" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDV2</accession>
    <name>F6BDV2_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_1124</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96663.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799264.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96663">
        <property type="protein sequence ID" value="AEF96663"/>
        <property type="gene ID" value="Metig_1124"/>
    </dbReference>
    <dbReference type="GeneID" id="10643982"/>
    <dbReference type="KEGG" id="mig:Metig_1124"/>
    <dbReference type="HOGENOM" id="CLU_1029010_0_0_2"/>
    <dbReference type="OMA" id="DANIDIY"/>
    <dbReference type="OrthoDB" id="127440at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF96663.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="270" mass="30451" checksum="2ACEEC4C5B9C76C0" modified="2011-07-27" version="1">MKLCSKGILKSFAALVILGMVGVVFATPVAISESNKDITVQKVNIKVVKNTPTDQIVKVNGNLIKYHTNGKVNILETKNKNIKSEYLIKVTKIKENKYRVDVYKDGKLIKSEITNNNPIKVNLVDIPIPRPHAYIDIHSDKYNYNVGEFGKVTIDVKNSLNGLILGHTIYYLRIPDGVRYYYIVDGPQPDAVRTLKEGDYIFVPYLGIVKGPCTLLIWQSLDYSDYKKRIVVKVKYLNKGVFIFKSGDAIKGAFWGIGDQDYDSFTVYAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-06-02" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDV4</accession>
    <name>F6BDV4_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_1126</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96665.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799266.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96665">
        <property type="protein sequence ID" value="AEF96665"/>
        <property type="gene ID" value="Metig_1126"/>
    </dbReference>
    <dbReference type="GeneID" id="10643984"/>
    <dbReference type="KEGG" id="mig:Metig_1126"/>
    <dbReference type="HOGENOM" id="CLU_1159062_0_0_2"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF96665.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="239" mass="27055" checksum="44601C076B2475E8" modified="2011-07-27" version="1">MKKIIFLLLGFLITLSYTTSVYAKVKVSPNLEVVINGKKIPMNAERIEKLEKMINENDTSWGEVLKTIAPEYWKSLSSTEKQIFQKMKFQSEIKGGSSKIVRGKLYLKPIQLNSYDRTTYLKTPTPLLLAGQSDISPSPNFPIIEYSADTRVVLPPYFNVAYISAGATLNRKISDSDSEMVAFVCTSATYKSYIEAVSNYYVIKPGYYRVIGTHAVEDPRCMPSSWTGISYTDWEYVNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDY3</accession>
    <name>F6BDY3_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Histone acetyltransferase, ELP3 family</fullName>
            <ecNumber evidence="15">2.3.1.48</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="ordered locus">Metig_1155</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>acetyl-CoA + H2O + S-adenosyl-L-methionine + uridine(34) in tRNA = 5'-deoxyadenosine + carboxymethyluridine(34) in tRNA + CoA + 2 H(+) + L-methionine</text>
            <dbReference type="Rhea" id="RHEA:61020"/>
            <dbReference type="Rhea" id="RHEA-COMP:10407"/>
            <dbReference type="Rhea" id="RHEA-COMP:11727"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74882"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="1">
            <dbReference type="Rhea" id="RHEA:61021"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="12">Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">tRNA modification.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ELP3 family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.48" evidence="15"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96694.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799295.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1155"/>
    <dbReference type="EnsemblBacteria" id="AEF96694">
        <property type="protein sequence ID" value="AEF96694"/>
        <property type="gene ID" value="Metig_1155"/>
    </dbReference>
    <dbReference type="GeneID" id="10644013"/>
    <dbReference type="KEGG" id="mig:Metig_1155"/>
    <dbReference type="HOGENOM" id="CLU_025983_2_1_2"/>
    <dbReference type="OMA" id="TFETRPD"/>
    <dbReference type="OrthoDB" id="7724at2157"/>
    <dbReference type="UniPathway" id="UPA00068">
        <property type="reaction ID" value="UER00107"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106261">
        <property type="term" value="F:tRNA uridine(34) acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.30.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039661">
        <property type="entry name" value="ELP3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034687">
        <property type="entry name" value="ELP3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaB/NifB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032432">
        <property type="entry name" value="Radical_SAM_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023404">
        <property type="entry name" value="rSAM_horseshoe"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11135">
        <property type="entry name" value="PTHR11135"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11135:SF0">
        <property type="entry name" value="PTHR11135:SF0"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16199">
        <property type="entry name" value="Radical_SAM_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005669">
        <property type="entry name" value="Hist_AcTrfase_ELP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00344">
        <property type="entry name" value="ELP3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01211">
        <property type="entry name" value="ELP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="11 15" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="9 12" id="KW-0408">Iron</keyword>
    <keyword evidence="10 12" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="7 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="15" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="Radical SAM core" evidence="14">
        <location>
            <begin position="76"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="domain" description="N-acetyltransferase" evidence="13">
        <location>
            <begin position="398"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="93"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="98"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="101"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR005669-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="AEF96694.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="537" mass="62263" checksum="1384899E7D19A7E5" modified="2011-07-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>F6BE07</accession>
    <name>F6BE07_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1180</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0111 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96718.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799317.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1180"/>
    <dbReference type="EnsemblBacteria" id="AEF96718">
        <property type="protein sequence ID" value="AEF96718"/>
        <property type="gene ID" value="Metig_1180"/>
    </dbReference>
    <dbReference type="GeneID" id="10644040"/>
    <dbReference type="KEGG" id="mig:Metig_1180"/>
    <dbReference type="HOGENOM" id="CLU_104916_1_1_2"/>
    <dbReference type="OMA" id="LPNMRRE"/>
    <dbReference type="OrthoDB" id="89024at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002727">
        <property type="entry name" value="DUF47"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038078">
        <property type="entry name" value="PhoU-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018445">
        <property type="entry name" value="Put_Phosphate_transp_reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36536">
        <property type="entry name" value="PTHR36536"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01865">
        <property type="entry name" value="PhoU_div"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00153">
        <property type="entry name" value="TIGR00153"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008591"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96718.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="206" mass="24466" checksum="A220094454EB94C9" modified="2011-07-27" version="1">MSIFFFKRKPEKEVMELIKYHINLSKKAIEILKEFLDDKDKEHLNKITSIEKEGDEIRKKAIFKLYEAFLPSMRRELNYSIEILDEVLDSVNHGAKIYDLMTFELDNNIKEKCKLVLDMSIEMLDCLVNAVNAFEEGGNFSEHIRIIKQREEEIDDIRHEIYRYMVSMEINNFWEGKLMSDFVDMITNISDLIEDVANEFQVIYSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>F6BE32</accession>
    <name>F6BE32_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Methanogen marker protein 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metig_0006</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the MtxX family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95568.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0006"/>
    <dbReference type="EnsemblBacteria" id="AEF95568">
        <property type="protein sequence ID" value="AEF95568"/>
        <property type="gene ID" value="Metig_0006"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0006"/>
    <dbReference type="HOGENOM" id="CLU_086562_0_0_2"/>
    <dbReference type="OMA" id="GVDEGWT"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016764">
        <property type="entry name" value="MeTrfase_MtxX_xsu"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF019709">
        <property type="entry name" value="Methyltransf_MtxX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03270">
        <property type="entry name" value="methan_mark_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEF95568.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="250" mass="28566" checksum="E72E0A0A8CD4D677" modified="2011-07-27" version="1">MCSDSMYAIGIGEKKDEVLKAYNKLREEGIEVELINNPKTLVDKLLNREIDGAVRGSLSSSKVIPYLREKVGKFYRASILKNPFNGEIFLLSPVGIDDIREDKEGRVKDKIELINYAVNFLKRNNIEPKIALLSGGRLSDYGRSREVDKTIEECEEILRHFKKTDEKLDIRHNGILIEEVLKKGCNIIIAPDGISGNLMFRCLGLVCNLEGYGAILLSKEEINFFDTSRNATWKRYYNAIKFMEKLIKIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F6BE55</accession>
    <name>F6BE55_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Dihydropteroate synthase-related protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0030</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95591.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798201.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0030"/>
    <dbReference type="EnsemblBacteria" id="AEF95591">
        <property type="protein sequence ID" value="AEF95591"/>
        <property type="gene ID" value="Metig_0030"/>
    </dbReference>
    <dbReference type="GeneID" id="10642865"/>
    <dbReference type="KEGG" id="mig:Metig_0030"/>
    <dbReference type="HOGENOM" id="CLU_041129_0_0_2"/>
    <dbReference type="OMA" id="DMIDLGM"/>
    <dbReference type="OrthoDB" id="58231at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004150">
        <property type="term" value="F:dihydroneopterin aldolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006760">
        <property type="term" value="P:folic acid-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006156">
        <property type="entry name" value="Dihydroneopterin_aldolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005236">
        <property type="entry name" value="Dihydropt_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011005">
        <property type="entry name" value="Dihydropteroate_synth-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025595">
        <property type="entry name" value="DUF4346"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000489">
        <property type="entry name" value="Pterin-binding_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42844">
        <property type="entry name" value="PTHR42844"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14251">
        <property type="entry name" value="DUF4346"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00809">
        <property type="entry name" value="Pterin_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51717">
        <property type="entry name" value="SSF51717"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00284">
        <property type="entry name" value="TIGR00284"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50972">
        <property type="entry name" value="PTERIN_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pterin-binding" evidence="1">
        <location>
            <begin position="142"/>
            <end position="389"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF95591.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="524" mass="59943" checksum="1459DFB949250FFD" modified="2011-07-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>F6BE76</accession>
    <name>F6BE76_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ATPase_AAA_core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1215</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96753.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799352.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96753">
        <property type="protein sequence ID" value="AEF96753"/>
        <property type="gene ID" value="Metig_1215"/>
    </dbReference>
    <dbReference type="GeneID" id="10644075"/>
    <dbReference type="KEGG" id="mig:Metig_1215"/>
    <dbReference type="HOGENOM" id="CLU_063816_1_1_2"/>
    <dbReference type="OMA" id="ECDEANA"/>
    <dbReference type="OrthoDB" id="38516at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014555">
        <property type="entry name" value="RecF-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13304">
        <property type="entry name" value="AAA_21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF029347">
        <property type="entry name" value="RecF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATPase_AAA_core" evidence="1">
        <location>
            <begin position="26"/>
            <end position="331"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96753.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="380" mass="44453" checksum="86E5DB9930C06021" modified="2011-07-27" version="1">MEKLKWIKIENFRAFKKAEISDFGDINVFIGKNNTGKSTLLESIYLNLAQNNKDLLGIYPFRAIFSRRGISGIGRVRYSEIDDSEDVITSIVYYLFHSINIDKEELNKLMSSINSNLNEFELNAYFGEFPEDILKIFFREYVGYYSGDRDDIQPQIIISDGNNDILFSVVEKLPRNEIIYRIHSKSFLRFHIKRQNVLMVDEYIMRLRTLHSSPMFSFIKRIERYSKIDKEHLSKFLSDQLNTEIVDIIPKIFDIFLLLKDNKQMPVSLLGDGTKMALSYYYALSLEDTYVLLEEPENHLHPKLMDKIIDLIINSSQKNQIFITTHNLEFLQKILEKSYEKNINLKVFAFENLVDGIPNIEVYECDEANAALNKIGVDLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-06-02" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>F6BE91</accession>
    <name>F6BE91_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_1231</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96768.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799367.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96768">
        <property type="protein sequence ID" value="AEF96768"/>
        <property type="gene ID" value="Metig_1231"/>
    </dbReference>
    <dbReference type="GeneID" id="10644092"/>
    <dbReference type="KEGG" id="mig:Metig_1231"/>
    <dbReference type="HOGENOM" id="CLU_139132_2_0_2"/>
    <dbReference type="OrthoDB" id="138185at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF96768.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="110" mass="12307" checksum="D82E69DBE113A3E1" modified="2011-07-27" version="1">MIVVEEGRYGVFLKHIKVGDDLVVIIGGGEKEHIGSVSLMDGKLKTINRDNHKDYVISEYAAKLIYEKIKKPVLVICGIHIDNAKKEEIDILVNNAKKCIELFLMKGGEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>F6BE94</accession>
    <name>F6BE94_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Methyl-coenzyme M reductase operon protein D</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1234</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96771.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799370.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1234"/>
    <dbReference type="EnsemblBacteria" id="AEF96771">
        <property type="protein sequence ID" value="AEF96771"/>
        <property type="gene ID" value="Metig_1234"/>
    </dbReference>
    <dbReference type="GeneID" id="10644097"/>
    <dbReference type="KEGG" id="mig:Metig_1234"/>
    <dbReference type="HOGENOM" id="CLU_118415_0_0_2"/>
    <dbReference type="OMA" id="ELEVFPH"/>
    <dbReference type="OrthoDB" id="94905at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015948">
        <property type="term" value="P:methanogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003901">
        <property type="entry name" value="Me_CoM_Rdtase_D"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02505">
        <property type="entry name" value="MCR_D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005636">
        <property type="entry name" value="McrD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03260">
        <property type="entry name" value="met_CoM_red_D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0484">Methanogenesis</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96771.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="168" mass="19382" checksum="260E1432CB5CA701" modified="2011-07-27" version="1">MIEVEVFPHRYLKATTTEKFLNKVYALETTERIIIHGQPLPKTVYYGPAKGTPVNHSERKIISVGGVPVELTVMAGRFWITLKDDSELDKLKEICEELFPFGYNIKVGKFLKDKPTVTDYIKYGERFVNEIDKRLIGITDPRSKFESAVKIIPKEEEKEDEEKSSKEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEA4</accession>
    <name>F6BEA4_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Tetrahydromethanopterin S-methyltransferase subunit G</fullName>
            <ecNumber evidence="9">2.1.1.86</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">N5-methyltetrahydromethanopterin--coenzyme M methyltransferase subunit G</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">mtrG</name>
        <name evidence="10" type="ordered locus">Metig_1244</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Part of a complex that catalyzes the formation of methyl-coenzyme M and tetrahydromethanopterin from coenzyme M and methyl-tetrahydromethanopterin. This is an energy-conserving, sodium-ion translocating step.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>5-methyl-5,6,7,8-tetrahydromethanopterin + coenzyme M + 2 Na(+)(in) = 5,6,7,8-tetrahydromethanopterin + methyl-coenzyme M + 2 Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:53492"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:58103"/>
            <dbReference type="ChEBI" id="CHEBI:58116"/>
            <dbReference type="ChEBI" id="CHEBI:58286"/>
            <dbReference type="ChEBI" id="CHEBI:58319"/>
            <dbReference type="EC" id="2.1.1.86"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="9">One-carbon metabolism; methanogenesis from CO(2); methyl-coenzyme M from 5,10-methylene-5,6,7,8-tetrahydromethanopterin: step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">The complex is composed of 8 subunits; MtrA, MtrB, MtrC, MtrD, MtrE, MtrF, MtrG and MtrH.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cell membrane</location>
            <topology evidence="9">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the MtrG family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.86" evidence="9"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96781.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799380.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1244"/>
    <dbReference type="EnsemblBacteria" id="AEF96781">
        <property type="protein sequence ID" value="AEF96781"/>
        <property type="gene ID" value="Metig_1244"/>
    </dbReference>
    <dbReference type="GeneID" id="10644107"/>
    <dbReference type="KEGG" id="mig:Metig_1244"/>
    <dbReference type="HOGENOM" id="CLU_191926_0_0_2"/>
    <dbReference type="OMA" id="PQVIMDP"/>
    <dbReference type="OrthoDB" id="130315at2157"/>
    <dbReference type="UniPathway" id="UPA00640">
        <property type="reaction ID" value="UER00698"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030269">
        <property type="term" value="F:tetrahydromethanopterin S-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019386">
        <property type="term" value="P:methanogenesis, from carbon dioxide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01500">
        <property type="entry name" value="MtrG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005866">
        <property type="entry name" value="THM_MeTrfase_su_G"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04210">
        <property type="entry name" value="MtrG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006500">
        <property type="entry name" value="MtrG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01149">
        <property type="entry name" value="mtrG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 9" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 9" id="KW-0484">Methanogenesis</keyword>
    <keyword evidence="3 9 10" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="2 9" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="49"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AEF96781.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="75" mass="8570" checksum="AA7AA5FA709BFF00" modified="2011-07-27" version="1">MAEIPSVVTPTKDFKRIQEELDKIEEMIENTNAELYQRAGKKVGRDVGIVYGLVLGIIFLTVFYNIVLPIFLQIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-09-29" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEC7</accession>
    <name>F6BEC7_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="3">ATPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_1267</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96804.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799402.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96804">
        <property type="protein sequence ID" value="AEF96804"/>
        <property type="gene ID" value="Metig_1267"/>
    </dbReference>
    <dbReference type="GeneID" id="10644130"/>
    <dbReference type="KEGG" id="mig:Metig_1267"/>
    <dbReference type="HOGENOM" id="CLU_041137_3_0_2"/>
    <dbReference type="OMA" id="YNEDKFH"/>
    <dbReference type="OrthoDB" id="21452at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011579">
        <property type="entry name" value="ATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004256">
        <property type="entry name" value="DUF234_ATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011335">
        <property type="entry name" value="Restrct_endonuc-II-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01637">
        <property type="entry name" value="ATPase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03008">
        <property type="entry name" value="DUF234"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52980">
        <property type="entry name" value="SSF52980"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATPase_2" evidence="1">
        <location>
            <begin position="2"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="domain" description="DUF234" evidence="2">
        <location>
            <begin position="298"/>
            <end position="395"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF96804.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="442" mass="52604" checksum="84A52B027079B20E" modified="2011-07-27" version="1">MFINREEELKALSEKLNSNTFEFVVIYGRRRIGKTKLALKSVENKEHIYYLAVEGDNLKHFKRYASKVVPTIEYAREDWEAYFNFLKDKIIIIDEFPNLIKENPNILSLFQRIVDVHLKDTKTKLIILGSSISMMGEKVLSYKSPLYGRKTGVLKIKPLKFKHLKEFFPNATWKELVEIYGFADGIPYYLEKIKLPFWDYLEEEIKRVDSFLKYEVDFLMKYEFEEPTTYKKILEAISFGNHSLGEIKNYLGIKHSDITPYLKNLIETEFIEREIPITESPKTKRGRYYIKDNFIAFYFRYIFPNLSAIEEGIFEIGEIKNDYNQYLGFVFEKVAKEFLIELNKNNKLPFKFLNIGRWWKKGEEVDLIALNKNEKRVLFVEVKWKNLKGKDVKKILNDLKRKSELVGLDDYEKYYAIIGRRVENKEDKDCLLFDLGDFDRCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>F6BED8</accession>
    <name>F6BED8_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="3">AIR synthase related protein domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_0038</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95599.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798208.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0038"/>
    <dbReference type="EnsemblBacteria" id="AEF95599">
        <property type="protein sequence ID" value="AEF95599"/>
        <property type="gene ID" value="Metig_0038"/>
    </dbReference>
    <dbReference type="GeneID" id="10642873"/>
    <dbReference type="KEGG" id="mig:Metig_0038"/>
    <dbReference type="HOGENOM" id="CLU_948680_0_0_2"/>
    <dbReference type="OMA" id="MEGPYPL"/>
    <dbReference type="OrthoDB" id="35382at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009030">
        <property type="term" value="F:thiamine-phosphate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009228">
        <property type="term" value="P:thiamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.650.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010918">
        <property type="entry name" value="PurM-like_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036676">
        <property type="entry name" value="PurM-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016188">
        <property type="entry name" value="PurM-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036921">
        <property type="entry name" value="PurM-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006283">
        <property type="entry name" value="ThiL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011414">
        <property type="entry name" value="UCP036541_AIR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30270">
        <property type="entry name" value="PTHR30270"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00586">
        <property type="entry name" value="AIRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02769">
        <property type="entry name" value="AIRS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036541">
        <property type="entry name" value="UCP036541_AIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55326">
        <property type="entry name" value="SSF55326"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56042">
        <property type="entry name" value="SSF56042"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AIRS" evidence="1">
        <location>
            <begin position="65"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="domain" description="AIRS_C" evidence="2">
        <location>
            <begin position="175"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF95599.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="292" mass="32615" checksum="B51DCF6711D06286" modified="2011-07-27" version="1">MENIEFEIKYAIGHMMETNYPRREFWNMDDKIPNLISGIRAGDDAVVVGKSVINMEGPYPLKLGSKTALIHTACDVVAMGAKPKFALNAIQAENEEEIKIAVDGLKKQSIGLDIPIIGGNTQTVKELKSCICVAVFGELITDKVVKDGNAKEDDVLIMLGHPVEGDIGERIYKAKNKFDTYLEIVKNIDVHACKDASRGGWLCNLLEMLVKARKGVEIFSIPYPRATRYLGTYIISVNKEDVKDVLDIALQNRCPAVTFGKIKKELGLTIGNKEYIDEEKMKELIKNFPYKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEF7</accession>
    <name>F6BEF7_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Methanogenesis marker protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_0057</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95618.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798227.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0057"/>
    <dbReference type="EnsemblBacteria" id="AEF95618">
        <property type="protein sequence ID" value="AEF95618"/>
        <property type="gene ID" value="Metig_0057"/>
    </dbReference>
    <dbReference type="GeneID" id="10642892"/>
    <dbReference type="KEGG" id="mig:Metig_0057"/>
    <dbReference type="HOGENOM" id="CLU_073250_0_0_2"/>
    <dbReference type="OMA" id="VPMVGGH"/>
    <dbReference type="OrthoDB" id="57531at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd02192">
        <property type="entry name" value="PurM-like3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.650.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017668">
        <property type="entry name" value="Methan_mark_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010918">
        <property type="entry name" value="PurM-like_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036676">
        <property type="entry name" value="PurM-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016188">
        <property type="entry name" value="PurM-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036921">
        <property type="entry name" value="PurM-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011413">
        <property type="entry name" value="UCP036540_AIR"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00586">
        <property type="entry name" value="AIRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02769">
        <property type="entry name" value="AIRS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036540">
        <property type="entry name" value="UCP036540_AIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55326">
        <property type="entry name" value="SSF55326"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56042">
        <property type="entry name" value="SSF56042"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03267">
        <property type="entry name" value="methan_mark_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AIRS" evidence="1">
        <location>
            <begin position="45"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="domain" description="AIRS_C" evidence="2">
        <location>
            <begin position="199"/>
            <end position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF95618.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="323" mass="35531" checksum="AA14D1EB22381900" modified="2011-07-27" version="1">MNLKKLAEEIKNFEGVIRKKEIKNVVSNFVFEEEYDFDIIVDFGDDAAVVGIDGENAILLAADGIWGKLLEADPWWAGYCSVLVNANDIAAMGGKPVAMTNIISIKDCDIGREVLSGVKEGVKKFGIPMVGGHTHPDAQCNVLDVSITGIVKKDCILRSDNAKVGDKIIFAYDLDGKLHEKFKLNWDTTTMKPKKIVREQLRALTIIGEEKLANSCKDISNPGAIGTLGMLLEVSKKGGVVDVTKIPKPEDIPLNHWLKVYPGTAFVFTAKEENVKSIVEILEDANITAEVCGEVIKDRKLIISDGKEKEIVFDFEKEYICGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEG7</accession>
    <name>F6BEG7_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Endonuclease NucS</fullName>
            <ecNumber evidence="2">3.1.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">nucS</name>
        <name evidence="3" type="ordered locus">Metig_0067</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Cleaves both 3' and 5' ssDNA extremities of branched DNA structures.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NucS endonuclease family.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="2"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95628.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0067"/>
    <dbReference type="EnsemblBacteria" id="AEF95628">
        <property type="protein sequence ID" value="AEF95628"/>
        <property type="gene ID" value="Metig_0067"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0067"/>
    <dbReference type="HOGENOM" id="CLU_069350_1_0_2"/>
    <dbReference type="OMA" id="RCSVDYA"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000014">
        <property type="term" value="F:single-stranded DNA endodeoxyribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1350.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00722">
        <property type="entry name" value="NucS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002793">
        <property type="entry name" value="Endonuclease_NucS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011856">
        <property type="entry name" value="tRNA_endonuc-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38814">
        <property type="entry name" value="PTHR38814"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01939">
        <property type="entry name" value="NucS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1 2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0540">Nuclease</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF95628.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="283" mass="32351" checksum="E8B1504DC70372A1" modified="2011-07-27" version="1">MDCGGSIGKVNYLVNPKAEDIVELLASGIVNDSILVFFAFCRVRYDGRAKSELEPGDRIIIIKPDGSFLVHKNTKREPVNWQPPGSVVSWEVHDGKLVLKSVRKKPREILQVELIKVYHACSFQCEDYEELSLTGSEAEMAELIFENPSLIEDGFKPLFKEKQINHGIIDILGKDKSGRWVVIELKRRRADLQAVSQLKRYVECLKYEYGEGNIRGILVAPSLTSGAKKLLEEENLEFRELKPPKKERLKENKQTTLDFYGYANNLNNLSSFQIHRSDRKIKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEJ3</accession>
    <name>F6BEJ3_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">DsrE family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_0093</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95654.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798263.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0093"/>
    <dbReference type="EnsemblBacteria" id="AEF95654">
        <property type="protein sequence ID" value="AEF95654"/>
        <property type="gene ID" value="Metig_0093"/>
    </dbReference>
    <dbReference type="GeneID" id="10642928"/>
    <dbReference type="KEGG" id="mig:Metig_0093"/>
    <dbReference type="HOGENOM" id="CLU_151801_1_0_2"/>
    <dbReference type="OMA" id="PYISEYA"/>
    <dbReference type="OrthoDB" id="91267at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027396">
        <property type="entry name" value="DsrEFH-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003787">
        <property type="entry name" value="Sulphur_relay_DsrE/F-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02635">
        <property type="entry name" value="DrsE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75169">
        <property type="entry name" value="SSF75169"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF95654.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="118" mass="12939" checksum="3C187211310AB942" modified="2011-07-27" version="1">MKTLTIILKSGSMMNMDPNFALKLADAALKKGYNVNLFCYGEGVTVIKKGQNPKRFPNIEKICRNLMEKGLKVAACSTCANARGIYEEDAIDGCKIGSLTNDLSQFIVDSDKVITLTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F6BER3</accession>
    <name>F6BER3_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_1323</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96860.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799457.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="SMR" id="F6BER3"/>
    <dbReference type="STRING" id="880724.Metig_1323"/>
    <dbReference type="EnsemblBacteria" id="AEF96860">
        <property type="protein sequence ID" value="AEF96860"/>
        <property type="gene ID" value="Metig_1323"/>
    </dbReference>
    <dbReference type="GeneID" id="10644186"/>
    <dbReference type="KEGG" id="mig:Metig_1323"/>
    <dbReference type="HOGENOM" id="CLU_2730360_0_0_2"/>
    <dbReference type="OMA" id="VTGVSNY"/>
    <dbReference type="OrthoDB" id="122525at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005001">
        <property type="entry name" value="Hfq"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17209">
        <property type="entry name" value="Hfq"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="SSF50182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF96860.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="76" mass="9043" checksum="0135DCB0A0BBBA6B" modified="2011-07-27" version="1">MMKQNKSRKPKQIPNFEYARRFVGKNVRIYLRNGEVLDAKVTGVSNYEIMATVMKDNEIKNMLIFKHAIDYIEFEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>F6BES6</accession>
    <name>F6BES6_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">UPF0147 protein Metig_1336</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_1336</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the UPF0147 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96873.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799470.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1336"/>
    <dbReference type="EnsemblBacteria" id="AEF96873">
        <property type="protein sequence ID" value="AEF96873"/>
        <property type="gene ID" value="Metig_1336"/>
    </dbReference>
    <dbReference type="GeneID" id="10644199"/>
    <dbReference type="KEGG" id="mig:Metig_1336"/>
    <dbReference type="HOGENOM" id="CLU_165882_1_0_2"/>
    <dbReference type="OMA" id="RTIIWEV"/>
    <dbReference type="OrthoDB" id="116790at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1440.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00342">
        <property type="entry name" value="UPF0147"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023130">
        <property type="entry name" value="Ta0600-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005354">
        <property type="entry name" value="UPF0147"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03685">
        <property type="entry name" value="UPF0147"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF96873.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="92" mass="10386" checksum="5F7EFC799D88FC60" modified="2011-07-27" version="1">MFAPKKLTPEEKLQQIAMMLDEIINDTTVPRNIRAAAEKAKETILKEGEEPIVKSATAIQILDDISDDPNMPLHTRTQIWSIVSELETVKNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-09-29" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEV6</accession>
    <name>F6BEV6_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Tetratricopeptide TPR_1 repeat-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_0132</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95692.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798301.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF95692">
        <property type="protein sequence ID" value="AEF95692"/>
        <property type="gene ID" value="Metig_0132"/>
    </dbReference>
    <dbReference type="GeneID" id="10642967"/>
    <dbReference type="KEGG" id="mig:Metig_0132"/>
    <dbReference type="HOGENOM" id="CLU_2021527_0_0_2"/>
    <dbReference type="OMA" id="YAWNNRA"/>
    <dbReference type="OrthoDB" id="470at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001440">
        <property type="entry name" value="TPR_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_repeat"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00515">
        <property type="entry name" value="TPR_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="SSF48452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="2">
        <location>
            <begin position="31"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="2">
        <location>
            <begin position="65"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="2">
        <location>
            <begin position="99"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF95692.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="135" mass="15594" checksum="C3633C7D4A156D89" modified="2011-07-27" version="1">MRITKTALVLGVISIFVSMSMCLNFGYDPKAVEWYNKGWDLQDSGKYLEAIECYDKALEIEPDFVEAWNNKGLALYELGRYSEAIKCYDKALEIDPNFAVAWYNKGLALKAIGKYQEARKCFEKAYELDPRLKHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEW0</accession>
    <name>F6BEW0_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_0136</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95696.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798305.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0136"/>
    <dbReference type="EnsemblBacteria" id="AEF95696">
        <property type="protein sequence ID" value="AEF95696"/>
        <property type="gene ID" value="Metig_0136"/>
    </dbReference>
    <dbReference type="GeneID" id="10642971"/>
    <dbReference type="KEGG" id="mig:Metig_0136"/>
    <dbReference type="HOGENOM" id="CLU_040551_4_1_2"/>
    <dbReference type="OMA" id="ICQKDAF"/>
    <dbReference type="OrthoDB" id="90481at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.160.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002838">
        <property type="entry name" value="AIM24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036983">
        <property type="entry name" value="AIM24_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016031">
        <property type="entry name" value="Trp_RNA-bd_attenuator-like_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01987">
        <property type="entry name" value="AIM24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51219">
        <property type="entry name" value="SSF51219"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00266">
        <property type="entry name" value="TIGR00266"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF95696.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="222" mass="23981" checksum="D0C1CD5F4D10B60D" modified="2011-07-27" version="1">MRGKDYEFEIKYTPSYSLLEVKLDNQEINAETGAMVYMDTSIKVNTNLKGGLLGALKRAIVGESVFINTFSGSGRIGFAPSAPGDIVHHVLDGTLYAQSGSYLASSPNIEIDTKFGGAKTFFGGKGLFLIKLEGEGDIFLSSFGAIEEIELNDESIIVDNGHLVAFTEGLDYKLTKLGSLKSAILGGEGKVYEFSGTGKIYIQSRSFEAFVGTIMPYIEPKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEX3</accession>
    <name>F6BEX3_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Dual specificity protein phosphatase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_0149</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95709.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0149"/>
    <dbReference type="EnsemblBacteria" id="AEF95709">
        <property type="protein sequence ID" value="AEF95709"/>
        <property type="gene ID" value="Metig_0149"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0149"/>
    <dbReference type="HOGENOM" id="CLU_1700308_0_0_2"/>
    <dbReference type="OMA" id="RERYCEC"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="SSF52799"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF95709.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="153" mass="17759" checksum="97EB6221C5B89F2B" modified="2011-07-27" version="1">MVIIMGKCRHNGEVSIFGVRPASFPYFPFHLKDKIGGFVILDELWLMRWCEIIEYPMRIPTLYVPIEDYGIPTVEDMDLIVDFIKYHVSKEREVVVSCIGGHGRTGTVLAVWTGLNGVENPIEYVRERYCECAVETQEQEEFVEYYLSIRNIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEY1</accession>
    <name>F6BEY1_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Iron (Metal) dependent repressor, DtxR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Metig_0159</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DtxR/MntR family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95717.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798326.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0159"/>
    <dbReference type="EnsemblBacteria" id="AEF95717">
        <property type="protein sequence ID" value="AEF95717"/>
        <property type="gene ID" value="Metig_0159"/>
    </dbReference>
    <dbReference type="GeneID" id="10642994"/>
    <dbReference type="KEGG" id="mig:Metig_0159"/>
    <dbReference type="HOGENOM" id="CLU_069532_4_1_2"/>
    <dbReference type="OMA" id="YCIIERF"/>
    <dbReference type="OrthoDB" id="110491at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046914">
        <property type="term" value="F:transition metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.60.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001367">
        <property type="entry name" value="Fe_dep_repressor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036421">
        <property type="entry name" value="Fe_dep_repressor_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022687">
        <property type="entry name" value="HTH_DTXR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022689">
        <property type="entry name" value="Iron_dep_repressor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02742">
        <property type="entry name" value="Fe_dep_repr_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01325">
        <property type="entry name" value="Fe_dep_repress"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00529">
        <property type="entry name" value="HTH_DTXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47979">
        <property type="entry name" value="SSF47979"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50944">
        <property type="entry name" value="HTH_DTXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH dtxR-type" evidence="5">
        <location>
            <begin position="1"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50944"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEF95717.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="132" mass="15268" checksum="E32FC55214BC8064" modified="2011-07-27" version="1">MPSANMEDYLEKIYLFTKNKNRPIKTTELAKLLNVKPSAITDMAKKMSKEGYIIYEPYVGISLSEEGEKIAKKTLRKHRIIETFLTNFLGMDLDKAYEEACKIEHVVSDETVERLYSFMNKPKTCPHGENIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEY5</accession>
    <name>F6BEY5_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Acetolactate synthase</fullName>
            <ecNumber evidence="6">2.2.1.6</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Metig_0163</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the TPP enzyme family.</text>
    </comment>
    <dbReference type="EC" id="2.2.1.6" evidence="6"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95721.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0163"/>
    <dbReference type="EnsemblBacteria" id="AEF95721">
        <property type="protein sequence ID" value="AEF95721"/>
        <property type="gene ID" value="Metig_0163"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0163"/>
    <dbReference type="HOGENOM" id="CLU_013748_3_1_2"/>
    <dbReference type="OMA" id="IGVKFGC"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003984">
        <property type="term" value="F:acetolactate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006082">
        <property type="term" value="P:organic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901576">
        <property type="term" value="P:organic substance biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044272">
        <property type="term" value="P:sulfur compound biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012000">
        <property type="entry name" value="Thiamin_PyroP_enz_cen_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012001">
        <property type="entry name" value="Thiamin_PyroP_enz_TPP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045229">
        <property type="entry name" value="TPP_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011766">
        <property type="entry name" value="TPP_enzyme-bd_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18968">
        <property type="entry name" value="PTHR18968"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02775">
        <property type="entry name" value="TPP_enzyme_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00205">
        <property type="entry name" value="TPP_enzyme_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02776">
        <property type="entry name" value="TPP_enzyme_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="SSF52467"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="SSF52518"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0786">Thiamine pyrophosphate</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="TPP_enzyme_N" evidence="5">
        <location>
            <begin position="21"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="domain" description="TPP_enzyme_M" evidence="3">
        <location>
            <begin position="200"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="domain" description="TPP_enzyme_C" evidence="4">
        <location>
            <begin position="363"/>
            <end position="500"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU362132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEF95721.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="512" mass="57183" checksum="42318F7ACD6081E5" modified="2011-07-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F6BF02</accession>
    <name>F6BF02_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Methionine synthase vitamin-B12 independent</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0181</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95738.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798347.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0181"/>
    <dbReference type="EnsemblBacteria" id="AEF95738">
        <property type="protein sequence ID" value="AEF95738"/>
        <property type="gene ID" value="Metig_0181"/>
    </dbReference>
    <dbReference type="GeneID" id="10643016"/>
    <dbReference type="KEGG" id="mig:Metig_0181"/>
    <dbReference type="HOGENOM" id="CLU_040013_3_2_2"/>
    <dbReference type="OMA" id="DPDCGMR"/>
    <dbReference type="OrthoDB" id="21172at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003871">
        <property type="term" value="F:5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009086">
        <property type="term" value="P:methionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03311">
        <property type="entry name" value="CIMS_C_terminal_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002629">
        <property type="entry name" value="Met_Synth_C/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038071">
        <property type="entry name" value="UROD/MetE-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01717">
        <property type="entry name" value="Meth_synt_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51726">
        <property type="entry name" value="SSF51726"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Meth_synt_2" evidence="1">
        <location>
            <begin position="1"/>
            <end position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF95738.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="309" mass="34670" checksum="3C804C46268EB9FF" modified="2011-07-27" version="1">MITTVVGSYPVITKKPETIVEKIKNFLGMFDEYKYAIEKAVIDQLSAGIDIVSDGQVRGDMVEIFVNNMYGFEGKRVIGKIEYTKPIILNDIKYTLKILSKHGNGKGVKGIITGPCTIASSVRVEGYYSDNKDEKLIYDIAVALKKEVMAIQDYVKMIQIDEPILSTKLYDLDVARKAINLITKDIKVPVALHVCGDVVDIFEDLNEFNVDILDHEFASNRKNLEVLEIIEKKVGFGCVNTKSKKVEDVNEIKALIEEGIEILKNNDNLKNKDVKEFMLIDPDCGMRLLPIDIAYNKLKNMVAASKLIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>F6BF23</accession>
    <name>F6BF23_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">4Fe-4S ferredoxin iron-sulfur binding domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1358</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96893.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799490.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1358"/>
    <dbReference type="EnsemblBacteria" id="AEF96893">
        <property type="protein sequence ID" value="AEF96893"/>
        <property type="gene ID" value="Metig_1358"/>
    </dbReference>
    <dbReference type="GeneID" id="10644221"/>
    <dbReference type="KEGG" id="mig:Metig_1358"/>
    <dbReference type="HOGENOM" id="CLU_043374_3_3_2"/>
    <dbReference type="OMA" id="IKCMHCE"/>
    <dbReference type="OrthoDB" id="105930at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00037">
        <property type="entry name" value="Fer4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13247">
        <property type="entry name" value="Fer4_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="57"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96893.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="136" mass="15095" checksum="24B0E11035A473A1" modified="2011-07-27" version="1">MKVLANVELCVSCGKCERICPNNGIYVIDGIPIKCMHCEKSPCLNVCPEEAIERINGKVVLNKDKCVGCGLCMTVCPIGAIRFSLDVASKCNGCYDREEELCKLVCPTNALDELEHTIDNKQQNVISKFKKIYSYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>F6BF25</accession>
    <name>F6BF25_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 6">Cell division protein FtsZ</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">ftsZ</name>
        <name evidence="9" type="ordered locus">Metig_1360</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Essential cell division protein that forms a contractile ring structure (Z ring) at the future cell division site. The regulation of the ring assembly controls the timing and the location of cell division. One of the functions of the FtsZ ring is to recruit other cell division proteins to the septum to produce a new cell wall between the dividing cells. Binds GTP and shows GTPase activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer. Polymerizes to form a dynamic ring structure in a strictly GTP-dependent manner. Interacts directly with several other division proteins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
        <text evidence="5">Assembles at midcell at the inner surface of the cytoplasmic membrane.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5 6">Belongs to the FtsZ family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96895.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799492.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1360"/>
    <dbReference type="EnsemblBacteria" id="AEF96895">
        <property type="protein sequence ID" value="AEF96895"/>
        <property type="gene ID" value="Metig_1360"/>
    </dbReference>
    <dbReference type="GeneID" id="10644224"/>
    <dbReference type="KEGG" id="mig:Metig_1360"/>
    <dbReference type="HOGENOM" id="CLU_024865_0_1_2"/>
    <dbReference type="OMA" id="IMNQGGV"/>
    <dbReference type="OrthoDB" id="41117at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032153">
        <property type="term" value="C:cell division site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000917">
        <property type="term" value="P:division septum assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043093">
        <property type="term" value="P:FtsZ-dependent cytokinesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051258">
        <property type="term" value="P:protein polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02201">
        <property type="entry name" value="FtsZ_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1440">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00909">
        <property type="entry name" value="FtsZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000158">
        <property type="entry name" value="Cell_div_FtsZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020805">
        <property type="entry name" value="Cell_div_FtsZ_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045061">
        <property type="entry name" value="FtsZ/CetZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024757">
        <property type="entry name" value="FtsZ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008280">
        <property type="entry name" value="Tub_FtsZ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037103">
        <property type="entry name" value="Tubulin/FtsZ-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018316">
        <property type="entry name" value="Tubulin/FtsZ_2-layer-sand-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036525">
        <property type="entry name" value="Tubulin/FtsZ_GTPase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003008">
        <property type="entry name" value="Tubulin_FtsZ_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30314">
        <property type="entry name" value="PTHR30314"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12327">
        <property type="entry name" value="FtsZ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00091">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00423">
        <property type="entry name" value="CELLDVISFTSZ"/>
    </dbReference>
    <dbReference type="SMART" id="SM00864">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00865">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52490">
        <property type="entry name" value="SSF52490"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55307">
        <property type="entry name" value="SSF55307"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00065">
        <property type="entry name" value="ftsZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01134">
        <property type="entry name" value="FTSZ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01135">
        <property type="entry name" value="FTSZ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="5 6 9" id="KW-0132">Cell division</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 5 6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2 5 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5 6" id="KW-0717">Septation</keyword>
    <feature type="domain" description="Tubulin" evidence="7">
        <location>
            <begin position="27"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="domain" description="Tubulin_C" evidence="8">
        <location>
            <begin position="219"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="35"/>
            <end position="39"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="122"/>
            <end position="124"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="153"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="156"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="199"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AEF96895.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="363" mass="38460" checksum="528F28F9692DB31B" modified="2011-07-27" version="1">MRLVREALSKNNDELRKNLKDDFGEARILVVGCGGAGNNTIDRLMEIGIEGAETIAINTDKQHLEAIKADKKILIGSTLTRGLGAGGYPEIGRKAAELAKNVLEDVLKGADLVFVAAGMGGGTGTGSAPVVAEIAKENGAVVIGVVTYPFKIERARLKKADEGIERLTESCDTVIVIDNNKLVDLVPNLPMNDAFKVADEIIAQAVKGITETISVPSLINIDYADVKAVMTDGGVAMIGVGEVDSDSKGDRVQTVVKETLECPLLDIDYKGAKGAIIHITGGPDLTLKEANDIGEGITESLDPNANVIWGARIDPSMEGCIRVMAIITGVKSPNILGKDRGPKRIWPKPERKKTGSLGIDYIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>F6BF73</accession>
    <name>F6BF73_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ABC-type transporter, periplasmic subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Metig_1408</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial solute-binding protein 5 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96943.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799539.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1408"/>
    <dbReference type="EnsemblBacteria" id="AEF96943">
        <property type="protein sequence ID" value="AEF96943"/>
        <property type="gene ID" value="Metig_1408"/>
    </dbReference>
    <dbReference type="GeneID" id="10644281"/>
    <dbReference type="KEGG" id="mig:Metig_1408"/>
    <dbReference type="HOGENOM" id="CLU_017028_7_5_2"/>
    <dbReference type="OMA" id="CMKQMQE"/>
    <dbReference type="OrthoDB" id="6658at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030678">
        <property type="entry name" value="Peptide/Ni-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039424">
        <property type="entry name" value="SBP_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023765">
        <property type="entry name" value="SBP_5_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000914">
        <property type="entry name" value="SBP_5_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30290">
        <property type="entry name" value="PTHR30290"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00496">
        <property type="entry name" value="SBP_bac_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002741">
        <property type="entry name" value="MppA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01040">
        <property type="entry name" value="SBP_BACTERIAL_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="domain" description="SBP_bac_5" evidence="4">
        <location>
            <begin position="98"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="28"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="29"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEF96943.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="538" mass="61167" checksum="C5A30C93E8D29F98" modified="2011-07-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>F6BF76</accession>
    <name>F6BF76_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Oligopeptide/dipeptide ABC transporter, ATPase subunit</fullName>
            <ecNumber evidence="6">3.6.3.24</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Metig_1411</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.6.3.24" evidence="6"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96946.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799542.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1411"/>
    <dbReference type="EnsemblBacteria" id="AEF96946">
        <property type="protein sequence ID" value="AEF96946"/>
        <property type="gene ID" value="Metig_1411"/>
    </dbReference>
    <dbReference type="GeneID" id="10644284"/>
    <dbReference type="KEGG" id="mig:Metig_1411"/>
    <dbReference type="HOGENOM" id="CLU_000604_1_23_2"/>
    <dbReference type="OMA" id="HLTACHY"/>
    <dbReference type="OrthoDB" id="43111at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015833">
        <property type="term" value="P:peptide transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013563">
        <property type="entry name" value="Oligopep_ABC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08352">
        <property type="entry name" value="oligo_HPY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01727">
        <property type="entry name" value="oligo_HPY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="5">
        <location>
            <begin position="5"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="41"/>
            <end position="48"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEF96946.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="316" mass="35188" checksum="FD793CC54B7E8FA1" modified="2011-07-27" version="1">MGNLLVVNDLHVDFLLGDIKVKAVNGVNLEIKEGETLCLIGESGSGKSVLGLSILKLLPSNVNISGSILFNNKDLLKLPEKELRKIRGKEIAWVPQSPSTSLNPVLKVGYQVAEPMMLHLNMDKKSAWSKAVNLLNFFGIYPADKRAEEYPHQFSGGMRQRALVAMGTSTKPKLIIADEPTKGVDLIKKVNVVKLFEQIKRNNDKLSLLLITHDIPFAKKLADRVGVMYCGQVVEVSNAETFFDEPLHPYSKALINALPSRGLKPIKGSPPSMVNPPKGCKFHPRCEFATEKCRNEEPPFIEYNGNFVRCHLYDKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F6BA65</accession>
    <name>F6BA65_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0198</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95755.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798364.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0198"/>
    <dbReference type="EnsemblBacteria" id="AEF95755">
        <property type="protein sequence ID" value="AEF95755"/>
        <property type="gene ID" value="Metig_0198"/>
    </dbReference>
    <dbReference type="GeneID" id="10643033"/>
    <dbReference type="KEGG" id="mig:Metig_0198"/>
    <dbReference type="HOGENOM" id="CLU_565769_0_0_2"/>
    <dbReference type="OMA" id="HKIAIGN"/>
    <dbReference type="OrthoDB" id="21208at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007503">
        <property type="entry name" value="DUF530"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04409">
        <property type="entry name" value="DUF530"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="10"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF95755.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="479" mass="56222" checksum="45B6B979476C23E4" modified="2011-07-27" version="1">MESSELIRESNEFLDNLKRLKFEIEELLKKKLEDSVIEEYAKKLEENLKILEELKEKMELLGFDTPYTNVGKLKGFDSDELYEIMNYTSYLRRIANEKKGVLERIRHSVVSHKIALGHLLEDIGNKKIIQHLPYDGSYKISITGLSPYLINAYKEMLNILQSQGKGVVTSITLSLIVFKDGKREFKRIKIEDENYEDYIKKHYKDAIITSMKRNYSKNKLIDDQYVRRVLAVGYLNAYKDDIEKVIRDKLNELLTGEQKKMLETYKKIVGVEEEEDYEGGIFDMRVLDEKKVRELETIEKLEKEGLYRNGRPIEDLKIALDTEKEISKKVATEILTEQLSRDIFMYYLHKSPDERTRSNLFPSIMTTPSKAHLKWMKVSGIDVPKVLDLKFLLEKELPKYNIPLKDLGGLVLYLVYDWDKVEEFKFKKKNVEDLLKKIAPIESIKDILKDKDVDISKIEKYSKVKKEKTKKFLEALGKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>F6BA77</accession>
    <name>F6BA77_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">CRISPR-associated protein Csc2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_0210</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95767.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0210"/>
    <dbReference type="EnsemblBacteria" id="AEF95767">
        <property type="protein sequence ID" value="AEF95767"/>
        <property type="gene ID" value="Metig_0210"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0210"/>
    <dbReference type="HOGENOM" id="CLU_782145_0_0_2"/>
    <dbReference type="OMA" id="EWAYSIR"/>
    <dbReference type="OrthoDB" id="92065at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017574">
        <property type="entry name" value="CRISPR-assoc_prot_Csc2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18320">
        <property type="entry name" value="Csc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03157">
        <property type="entry name" value="cas_Csc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF95767.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="350" mass="40753" checksum="115A7BE1419C2E92" modified="2011-07-27" version="1">MTMAKQKKLVENGYKKYVEYDENIVNEFREKLYSIIPEEYFQETYTKRTPNVIKVATIRTTTGYLINRSTEPDEVISTTIGNKDVVVIPSRKLKSREKLTGLVLCRKFKVIHPEVEYNFIDKQEHLANPNSLVFGDSVTKTNEAVALPSRVIYEWAYSIRDKDEITEELTHNALSEDGTMWDKNEGSQRQSLYGIQYIKPGVYFPHFLTFYDITPEGFIHALISHLKTTRYGAQSNVMDANMKNEIIAIALDTFEPPVSSYLISKEFEGEVNFENVKEFVKNKLRENSSILIENEKLEELLKLIDEYLKDEEKLKQLYLKHLNDCINYLVECKIIKKEDVIKKLLEEMGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-09-29" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F6BA87</accession>
    <name>F6BA87_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Tetratricopeptide repeat-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0220</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95777.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798386.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF95777">
        <property type="protein sequence ID" value="AEF95777"/>
        <property type="gene ID" value="Metig_0220"/>
    </dbReference>
    <dbReference type="GeneID" id="10643055"/>
    <dbReference type="KEGG" id="mig:Metig_0220"/>
    <dbReference type="HOGENOM" id="CLU_620570_0_0_2"/>
    <dbReference type="OrthoDB" id="44881at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_repeat"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="SSF48452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="147"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF95777.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="441" mass="52267" checksum="0A3A52EDD6F65662" modified="2011-07-27" version="1">MNSLNKDEKINEFINYIGEGAFFYIKLLMAIGKCIEDNLSDEDDDLVDKLLFDENKDEDFDDDLVYEMAEETYKQWYKAWDLFKELKIKDSNLLEFYDKYNVDLERWMDDFVLVLKVLTRKDKKYVEKAKEIINYLIEHSSDEESKLIYLYELGHLYSLIGEYKKAEDIYREVIDKLPDYPITYIEYAQLLIETGRFGDAEKIMERYYENCEMVCDALYESVIGAIYLNLKERGINQISEKLRSYYNLRDKITDVTDRLYDEVGAERFKKSIKILEFPFENNTFFADEEEELAILADFMIFGSIDGVRTIDNVDESKYDNEILDALKKSKYSLFKVVSTNPKEHIVEIEDVFENKRYTVMDVMMSKTVDVGYLFTSRYVPFENYGIILVCGYAFSPKSETILLKKFKKLMKKLNYGDEETKKFIALHKLGKRYGDLMTALW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>F6BA91</accession>
    <name>F6BA91_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">4Fe-4S ferredoxin iron-sulfur binding domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0224</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95781.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0224"/>
    <dbReference type="EnsemblBacteria" id="AEF95781">
        <property type="protein sequence ID" value="AEF95781"/>
        <property type="gene ID" value="Metig_0224"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0224"/>
    <dbReference type="HOGENOM" id="CLU_108340_0_0_2"/>
    <dbReference type="OMA" id="YCHDFCP"/>
    <dbReference type="OrthoDB" id="108931at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12838">
        <property type="entry name" value="Fer4_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="42"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="84"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF95781.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="161" mass="18515" checksum="BE01B6BC46D93191" modified="2011-07-27" version="1">MLKNLAKIFITGIYENLERILFGKDRYTSIEMRNKILEGIELPRMVFEELCIGCGGCANACPTNAIEMVPIEPVKITEYYTKDKIPKIDAEKCVYCLYCHDFCPVFAVFNEISPIHPRHVGDECIKVDLSQVLKKPVEIREEQIKKIAKILSINLRHILTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAC1</accession>
    <name>F6BAC1_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Beta-lactamase domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_0255</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95811.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798420.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0255"/>
    <dbReference type="EnsemblBacteria" id="AEF95811">
        <property type="protein sequence ID" value="AEF95811"/>
        <property type="gene ID" value="Metig_0255"/>
    </dbReference>
    <dbReference type="GeneID" id="10643090"/>
    <dbReference type="KEGG" id="mig:Metig_0255"/>
    <dbReference type="HOGENOM" id="CLU_009673_5_1_2"/>
    <dbReference type="OMA" id="YLKAMLG"/>
    <dbReference type="OrthoDB" id="39954at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022712">
        <property type="entry name" value="Beta_Casp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011108">
        <property type="entry name" value="RMMBL"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10996">
        <property type="entry name" value="Beta-Casp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07521">
        <property type="entry name" value="RMMBL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01027">
        <property type="entry name" value="Beta-Casp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="SSF56281"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Lactamase_B" evidence="1">
        <location>
            <begin position="13"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="domain" description="Beta-Casp" evidence="2">
        <location>
            <begin position="232"/>
            <end position="346"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF95811.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="426" mass="48345" checksum="0913219596B43CD1" modified="2011-07-27" version="1">MKITFRGAAMEVGRSCIEIDSGESKILLDCGVKLTGEGPKYPILDNIKVDGVFVSHAHLDHVGALPILTHNQINAPIHSTPLTKEITKVLLKDSLKIEMVEHISPKFNMHDIKKCINLHIPTYYNKRRVFKDFVYEYFDAGHIPGSASILLDYGNKKILYTGDIKVRDTRLVKGADLSYANEDIDVLIIESTYGNSIHPDREMTEKRFLQKIKETINRGGVALIPVFAVNRAQEILLILAEEKFDVPIFVDGMAIEITEIMLRHSEYLRDAKKLEKAYSRVYVVKSRDEREKVIEYLKENGGIVVTTAGMLDGGPILHYLSEMWDNPENSLILTGYQVEDTNGRYLIETGYLIINGQEIKPKLEVTLFDFSAHAGMDELHEFVNKVNPETLIIQHGDELEVLTFKKWAIEKGFNVYTPKLGEVIRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAD7</accession>
    <name>F6BAD7_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Protease HtpX homolog</fullName>
            <ecNumber evidence="11">3.4.24.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">htpX</name>
        <name evidence="13" type="ordered locus">Metig_0271</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="11">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Cell membrane</location>
            <topology evidence="11">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the peptidase M48B family.</text>
    </comment>
    <dbReference type="EC" id="3.4.24.-" evidence="11"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95827.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798436.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0271"/>
    <dbReference type="EnsemblBacteria" id="AEF95827">
        <property type="protein sequence ID" value="AEF95827"/>
        <property type="gene ID" value="Metig_0271"/>
    </dbReference>
    <dbReference type="GeneID" id="10643106"/>
    <dbReference type="KEGG" id="mig:Metig_0271"/>
    <dbReference type="HOGENOM" id="CLU_042266_3_0_2"/>
    <dbReference type="OMA" id="AVCCTEG"/>
    <dbReference type="OrthoDB" id="62484at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00188">
        <property type="entry name" value="Pept_M48_protease_HtpX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022919">
        <property type="entry name" value="Pept_M48_protease_HtpX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001915">
        <property type="entry name" value="Peptidase_M48"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01435">
        <property type="entry name" value="Peptidase_M48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 11" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 11" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="3 11 13" id="KW-0645">Protease</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="7 11" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="31"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="140"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="177"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_M48" evidence="12">
        <location>
            <begin position="64"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="active site" evidence="11">
        <location>
            <position position="131"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="130"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="134"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="206"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AEF95827.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="292" mass="32349" checksum="AF42637EA72D758A" modified="2011-07-27" version="1">MFLENVKTIILLAVLTGMLYGICYLLHIPPVFAIIIALVPNLIAYFFCDKFVLWSYNARIVDEHEMPQLHRMVEKIAIKAGIPKPRVAIIETPTPNAFATGRSPKNAVVAVTTGILNLLTPEELEGVIAHEIGHIVHRDILISSIVATIAGAIMYIADWLQWSLLFGFGRDEEEESPLSLIATLAFMILAPIAATLIQLAISRQREFYADEEGGRLSNPIYLANALAKLEKGVAVYPMEKGSPSTAHMFIVNPFKGEAIAKLFSTHPPTEERIKRLLELARKMGIYTPTIGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAE2</accession>
    <name>F6BAE2_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Methyltransferase type 11</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_0276</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95832.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798441.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0276"/>
    <dbReference type="EnsemblBacteria" id="AEF95832">
        <property type="protein sequence ID" value="AEF95832"/>
        <property type="gene ID" value="Metig_0276"/>
    </dbReference>
    <dbReference type="GeneID" id="10643111"/>
    <dbReference type="KEGG" id="mig:Metig_0276"/>
    <dbReference type="HOGENOM" id="CLU_074702_1_0_2"/>
    <dbReference type="OMA" id="DVVYSIH"/>
    <dbReference type="OrthoDB" id="119044at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF95832.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="204" mass="22990" checksum="F7C090D1AEA70AA2" modified="2011-07-27" version="1">MKKKHLEIILDQLKPHPKPKVNLEQYTIEGRLASEILFFAKSDIEGSAVVDLGCGTGRLAIGAKLLNAKKVIGIDIDEESIEVAKENAKKANVDVDFYCMDVADVDVNFIKEICGDLKIVVVQNPPFGAQKRHADRIFLDKALEIGDVIYTIHNAATKDFVVKYVNEKGRTITHIFQGSFRIPHIYEFHKKEVVYIPVNIFRVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAF4</accession>
    <name>F6BAF4_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_1432</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96967.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799563.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1432"/>
    <dbReference type="EnsemblBacteria" id="AEF96967">
        <property type="protein sequence ID" value="AEF96967"/>
        <property type="gene ID" value="Metig_1432"/>
    </dbReference>
    <dbReference type="GeneID" id="10644305"/>
    <dbReference type="KEGG" id="mig:Metig_1432"/>
    <dbReference type="HOGENOM" id="CLU_108341_0_0_2"/>
    <dbReference type="OMA" id="CTEGMLD"/>
    <dbReference type="OrthoDB" id="68097at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016736">
        <property type="entry name" value="MJ1481_archaea"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09873">
        <property type="entry name" value="DUF2100"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF96967.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="217" mass="24986" checksum="80F1296A9DAC5FB5" modified="2011-07-27" version="1">MIAKRVEDSKNLIIKAISTISEIERMGKPSADSKTISYKDAKAGKINVDEFKKAIYALIEADEFLYKKAPLHELNEEEAKEFCRLILKAERHLNNVLKDFGFEFEEKEIDKNALYIVSNKKLFRKLKDKNPDLNVICTEGMLDIEDMKTINPNIPEKALEGIKKKIEITKNNIAKRIEKTKPSKVVVVVEDKADELIYNRAKELYNADKIDVNELLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-09-29" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAI7</accession>
    <name>F6BAI7_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Metallophosphoesterase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1466</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97000.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF97000">
        <property type="protein sequence ID" value="AEF97000"/>
        <property type="gene ID" value="Metig_1466"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_1466"/>
    <dbReference type="HOGENOM" id="CLU_041441_5_0_2"/>
    <dbReference type="OMA" id="AVPGNCD"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP_ApaH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="SSF56300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metallophos" evidence="1">
        <location>
            <begin position="3"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF97000.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="211" mass="23872" checksum="9B0DD77421F1D6C9" modified="2011-07-27" version="1">MGLTDLHGKLINFNKILRYKPDVILISGDITHFGRDFRIIDLLKSVNENVKVLAVPGNCDTKEAIEKLNKYNLNIDEKCNEINGIKFVGIGGSNRTPFNTPNEYEDEELFNKFLKGIKDIDKNTLKNNFILVTHAPPKNTMADRVGDTHVGSEAVRKIIEEYQPILCACGHIHESRCIDKIGETIIVNPSPDAFFVFDTKTKYLEIIELKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-06-02" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAI8</accession>
    <name>F6BAI8_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Acylphosphatase</fullName>
            <ecNumber evidence="1">3.6.1.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Metig_1467</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>an acyl phosphate + H2O = a carboxylate + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:14965"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29067"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:59918"/>
            <dbReference type="EC" id="3.6.1.7"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the acylphosphatase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.7" evidence="1"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97001.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799597.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF97001">
        <property type="protein sequence ID" value="AEF97001"/>
        <property type="gene ID" value="Metig_1467"/>
    </dbReference>
    <dbReference type="GeneID" id="10644340"/>
    <dbReference type="KEGG" id="mig:Metig_1467"/>
    <dbReference type="HOGENOM" id="CLU_1656874_0_0_2"/>
    <dbReference type="OrthoDB" id="111329at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003998">
        <property type="term" value="F:acylphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020456">
        <property type="entry name" value="Acylphosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001792">
        <property type="entry name" value="Acylphosphatase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036046">
        <property type="entry name" value="Acylphosphatase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017968">
        <property type="entry name" value="Acylphosphatase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47268">
        <property type="entry name" value="PTHR47268"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00708">
        <property type="entry name" value="Acylphosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54975">
        <property type="entry name" value="SSF54975"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00150">
        <property type="entry name" value="ACYLPHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51160">
        <property type="entry name" value="ACYLPHOSPHATASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acylphosphatase-like" evidence="4">
        <location>
            <begin position="4"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="121"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="19"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU004168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEF97001.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="159" mass="18758" checksum="792D46E189D4061D" modified="2011-07-27" version="1">MTITYELIIYGKVQHVGFRERLEDIGRGLGIDGIVYNYEDGSVRILANFPSERKKRLFKEFIKDLEEEDKLIKINKIEEKELNAYIEFPKGLNRISADDLLELNKKLDEGVKYIKLIFNELEEHKRLLIEIKDSMNELKEGINELNKKFDVLIDILKNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAL9</accession>
    <name>F6BAL9_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0292 protein Metig_0277</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_0277</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0292 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95833.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798442.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0277"/>
    <dbReference type="EnsemblBacteria" id="AEF95833">
        <property type="protein sequence ID" value="AEF95833"/>
        <property type="gene ID" value="Metig_0277"/>
    </dbReference>
    <dbReference type="GeneID" id="10643112"/>
    <dbReference type="KEGG" id="mig:Metig_0277"/>
    <dbReference type="HOGENOM" id="CLU_140789_0_0_2"/>
    <dbReference type="OMA" id="VMILTDF"/>
    <dbReference type="OrthoDB" id="99443at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01095">
        <property type="entry name" value="UPF0292"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006171">
        <property type="entry name" value="TOPRIM_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022972">
        <property type="entry name" value="UPF0292"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13662">
        <property type="entry name" value="Toprim_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00493">
        <property type="entry name" value="TOPRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50880">
        <property type="entry name" value="TOPRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Toprim" evidence="2">
        <location>
            <begin position="25"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF95833.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="134" mass="15672" checksum="39B2FA169847240C" modified="2011-07-27" version="1">MYSRMEYLIKLMEVIEELKEEVERGIPIIVEGKKDVKSLRNLGVDGIFITVSRTPIFEIADELIANNIREVILLTDFDKRGRQFARAIVDELQSRGIKVNTEIRKKIIKYSHGDLKDVESLYIYISRRIEGIRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAQ8</accession>
    <name>F6BAQ8_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="1 13">DNA ligase</fullName>
            <ecNumber evidence="13">6.5.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Polydeoxyribonucleotide synthase [ATP]</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">lig</name>
        <name evidence="16" type="ordered locus">Metig_0316</name>
    </gene>
    <organism evidence="17">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="13 14">Belongs to the ATP-dependent DNA ligase family.</text>
    </comment>
    <dbReference type="EC" id="6.5.1.1" evidence="13"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95872.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798481.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0316"/>
    <dbReference type="EnsemblBacteria" id="AEF95872">
        <property type="protein sequence ID" value="AEF95872"/>
        <property type="gene ID" value="Metig_0316"/>
    </dbReference>
    <dbReference type="GeneID" id="10643152"/>
    <dbReference type="KEGG" id="mig:Metig_0316"/>
    <dbReference type="HOGENOM" id="CLU_005138_6_0_2"/>
    <dbReference type="OMA" id="WLFEESY"/>
    <dbReference type="OrthoDB" id="52275at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003910">
        <property type="term" value="F:DNA ligase (ATP) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007049">
        <property type="term" value="P:cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071897">
        <property type="term" value="P:DNA biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00407">
        <property type="entry name" value="DNA_ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022865">
        <property type="entry name" value="DNA_ligae_ATP-dep_bac/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000977">
        <property type="entry name" value="DNA_ligase_ATP-dep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012309">
        <property type="entry name" value="DNA_ligase_ATP-dep_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012310">
        <property type="entry name" value="DNA_ligase_ATP-dep_cent"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016059">
        <property type="entry name" value="DNA_ligase_ATP-dep_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012308">
        <property type="entry name" value="DNA_ligase_ATP-dep_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036599">
        <property type="entry name" value="DNA_ligase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04679">
        <property type="entry name" value="DNA_ligase_A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01068">
        <property type="entry name" value="DNA_ligase_A_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04675">
        <property type="entry name" value="DNA_ligase_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117018">
        <property type="entry name" value="SSF117018"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00574">
        <property type="entry name" value="dnl1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00697">
        <property type="entry name" value="DNA_LIGASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00333">
        <property type="entry name" value="DNA_LIGASE_A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50160">
        <property type="entry name" value="DNA_LIGASE_A3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12 13" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3 13" id="KW-0132">Cell division</keyword>
    <keyword evidence="7 13" id="KW-0227">DNA damage</keyword>
    <keyword evidence="10 13" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="11 13" id="KW-0234">DNA repair</keyword>
    <keyword evidence="4 13" id="KW-0235">DNA replication</keyword>
    <keyword evidence="2 13 16" id="KW-0436">Ligase</keyword>
    <keyword evidence="9 13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA_LIGASE_A3" evidence="15">
        <location>
            <begin position="328"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="active site" description="N6-AMP-lysine intermediate" evidence="13">
        <location>
            <position position="250"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="248"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="255"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="270"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="299"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="340"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="432"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="438"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00013308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU004196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="AEF95872.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="576" mass="67225" checksum="21B4B308B5D7BAB1" modified="2011-07-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAR8</accession>
    <name>F6BAR8_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Molybdate ABC transporter, inner membrane subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Metig_0326</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cell membrane</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95882.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798491.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0326"/>
    <dbReference type="EnsemblBacteria" id="AEF95882">
        <property type="protein sequence ID" value="AEF95882"/>
        <property type="gene ID" value="Metig_0326"/>
    </dbReference>
    <dbReference type="GeneID" id="10643162"/>
    <dbReference type="KEGG" id="mig:Metig_0326"/>
    <dbReference type="HOGENOM" id="CLU_016047_14_1_2"/>
    <dbReference type="OMA" id="AQLTWDQ"/>
    <dbReference type="OrthoDB" id="97465at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015098">
        <property type="term" value="F:molybdate ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011867">
        <property type="entry name" value="ModB_ABC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02141">
        <property type="entry name" value="modB_ABC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0500">Molybdenum</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="7"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="50"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="88"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="121"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="202"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="230"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="7">
        <location>
            <begin position="50"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEF95882.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="256" mass="27908" checksum="7B01D8FFF4293FED" modified="2011-07-27" version="1">MQPITFRFLIGFSITVFILLIIMPFVALLFNILSNPLEELIDYKSILNPLILSVTTSLVSTIISLCVGIPLAYILTYKKFPLKDVFDTIVNLPLVVPPTVAGYLLLITFGRYGLIGYPLHLLGITIMFTTFAIVIAQTFVALPFMVRSIRASLQDIPSSLVDAAKTLGASEYEIFKEIILPLSKTGIISGIILTYARAMGEFGATVMVCGLLETLPIAIFNSAMGGNREIANILSLILIVISFGILTLFKRIAAKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAS0</accession>
    <name>F6BAS0_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_0328</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95884.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798493.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0328"/>
    <dbReference type="EnsemblBacteria" id="AEF95884">
        <property type="protein sequence ID" value="AEF95884"/>
        <property type="gene ID" value="Metig_0328"/>
    </dbReference>
    <dbReference type="GeneID" id="10643164"/>
    <dbReference type="KEGG" id="mig:Metig_0328"/>
    <dbReference type="HOGENOM" id="CLU_1623445_0_0_2"/>
    <dbReference type="OMA" id="ACGAGLK"/>
    <dbReference type="OrthoDB" id="104314at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF95884.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="164" mass="18028" checksum="4A3D6287CD08005C" modified="2011-07-27" version="1">MAVITIKKDFVEIKRKLMEKNVKTVMVIGCGICAKSSKTGGPEEVKEMKKMLIEEGFRIFEDENLPDSLEDGLCRYSAVEKLAKSVDVDSFDSILVLACGAGLKCIADNFKDKKIISGVNTIGIGIKEKLVCLACGDCGFDDGVCKRLAIIEEINKHLKRSYNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAS2</accession>
    <name>F6BAS2_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">3-dehydroquinate dehydratase</fullName>
            <shortName evidence="3">3-dehydroquinase</shortName>
            <ecNumber evidence="3">4.2.1.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Type I DHQase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="3">Type I dehydroquinase</fullName>
            <shortName evidence="3">DHQ1</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">aroD</name>
        <name evidence="4" type="ordered locus">Metig_0330</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Involved in the third step of the chorismate pathway, which leads to the biosynthesis of aromatic amino acids. Catalyzes the cis-dehydration of 3-dehydroquinate (DHQ) and introduces the first double bond of the aromatic ring to yield 3-dehydroshikimate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>3-dehydroquinate = 3-dehydroshikimate + H2O</text>
            <dbReference type="Rhea" id="RHEA:21096"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16630"/>
            <dbReference type="ChEBI" id="CHEBI:32364"/>
            <dbReference type="EC" id="4.2.1.10"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 3/7.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the type-I 3-dehydroquinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.10" evidence="3"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95886.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798495.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0330"/>
    <dbReference type="EnsemblBacteria" id="AEF95886">
        <property type="protein sequence ID" value="AEF95886"/>
        <property type="gene ID" value="Metig_0330"/>
    </dbReference>
    <dbReference type="GeneID" id="10643166"/>
    <dbReference type="KEGG" id="mig:Metig_0330"/>
    <dbReference type="HOGENOM" id="CLU_064444_2_1_2"/>
    <dbReference type="OMA" id="ATMAMGE"/>
    <dbReference type="OrthoDB" id="75928at2157"/>
    <dbReference type="UniPathway" id="UPA00053">
        <property type="reaction ID" value="UER00086"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003855">
        <property type="term" value="F:3-dehydroquinate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009073">
        <property type="term" value="P:aromatic amino acid family biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:cellular amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009423">
        <property type="term" value="P:chorismate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00502">
        <property type="entry name" value="DHQase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00214">
        <property type="entry name" value="AroD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018508">
        <property type="entry name" value="3-dehydroquinate_DH_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001381">
        <property type="entry name" value="DHquinase_I"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01487">
        <property type="entry name" value="DHquinase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01093">
        <property type="entry name" value="aroD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01028">
        <property type="entry name" value="DEHYDROQUINASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="3" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="2 3 4" id="KW-0456">Lyase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0704">Schiff base</keyword>
    <feature type="region of interest" description="3-dehydroquinate binding" evidence="3">
        <location>
            <begin position="29"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="3">
        <location>
            <position position="116"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with substrate" evidence="3">
        <location>
            <position position="142"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="56"/>
        </location>
        <ligand>
            <name>3-dehydroquinate</name>
            <dbReference type="ChEBI" id="CHEBI:32364"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="180"/>
        </location>
        <ligand>
            <name>3-dehydroquinate</name>
            <dbReference type="ChEBI" id="CHEBI:32364"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="200"/>
        </location>
        <ligand>
            <name>3-dehydroquinate</name>
            <dbReference type="ChEBI" id="CHEBI:32364"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="204"/>
        </location>
        <ligand>
            <name>3-dehydroquinate</name>
            <dbReference type="ChEBI" id="CHEBI:32364"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEF95886.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="218" mass="24689" checksum="C9D88064E7F5A6D6" modified="2011-07-27" version="1">MICIPIVDNNIDDALKNAEKALKEADIVEFRVDMLDDVNESAIEEMVKYPCIITVRADWEGGFWKKSNDERINLIKKAIECNAKFVDIELKEEKNKELVKFRDEIGSKTKIIISYHDFEKTPSCEELIKIVEEELKIGDIAKFAVMAKSKEDVLKVLNVINKYNGKIIGIAMGEEGKLTRILGVHFGSILTFASMEGKASAPGQIDVKKLKEIWKLIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAS7</accession>
    <name>F6BAS7_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0335</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95891.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF95891">
        <property type="protein sequence ID" value="AEF95891"/>
        <property type="gene ID" value="Metig_0335"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0335"/>
    <dbReference type="HOGENOM" id="CLU_1933261_0_0_2"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="5"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="27"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="61"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="94"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF95891.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="130" mass="15344" checksum="5EA7A5A81D103059" modified="2011-07-27" version="1">MIGIIFYLSVFIFSTIIAYFVPLNLDVLYVLILFFLILHSCIVVSFFRIFNKVSKKDIKMLTLYMASILIYGCISLHLLYLSIFKGFDDKIPKFISVSFILISIYIVIRYIKFRKGKNIKRHYLKIKHKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAU3</accession>
    <name>F6BAU3_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Taurine-transporting ATPase</fullName>
            <ecNumber evidence="5">3.6.3.36</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Metig_0351</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.6.3.36" evidence="5"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95907.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798516.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0351"/>
    <dbReference type="EnsemblBacteria" id="AEF95907">
        <property type="protein sequence ID" value="AEF95907"/>
        <property type="gene ID" value="Metig_0351"/>
    </dbReference>
    <dbReference type="GeneID" id="10643188"/>
    <dbReference type="KEGG" id="mig:Metig_0351"/>
    <dbReference type="HOGENOM" id="CLU_000604_1_22_2"/>
    <dbReference type="OMA" id="YSLFPWR"/>
    <dbReference type="OrthoDB" id="64935at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="4"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="40"/>
            <end position="47"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEF95907.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="254" mass="28864" checksum="AEC275340B36C0D9" modified="2011-07-27" version="1">MAKLELINITKKYITEKKEILAVDNVSLSVEENEFISIVGPSGCGKSTLLRIIAGLEKPTSGKVLLDGKEVKEPSAERGMVFQQYTLMPWRTVLKNVTFGLEIKKIPKEEQIKIAKKFIKMVGLEGFEDAYPYQLSGGMQQRVAIARTLANDPEIVLMDEPFGALDTQTRAILQNELLKIWQKEKKTVLFVTHSVDEAVYLSDRIVVMTARPGKIKKIIPIELKRPRDRTSIEFLEYKKKIVDELKDEVLKAMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAU9</accession>
    <name>F6BAU9_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">UPF0107 protein Metig_1502</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metig_1502</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the UPF0107 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97036.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799630.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1502"/>
    <dbReference type="EnsemblBacteria" id="AEF97036">
        <property type="protein sequence ID" value="AEF97036"/>
        <property type="gene ID" value="Metig_1502"/>
    </dbReference>
    <dbReference type="GeneID" id="10644375"/>
    <dbReference type="KEGG" id="mig:Metig_1502"/>
    <dbReference type="HOGENOM" id="CLU_141583_2_0_2"/>
    <dbReference type="OMA" id="CEPIVAT"/>
    <dbReference type="OrthoDB" id="108624at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd01356">
        <property type="entry name" value="AcnX_swivel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00078">
        <property type="entry name" value="UPF0107"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020794">
        <property type="entry name" value="AcnX_sub2_archaea_put"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012016">
        <property type="entry name" value="AcnX_sub2_put"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002840">
        <property type="entry name" value="AcnX_swivel_put"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01989">
        <property type="entry name" value="DUF126"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004966">
        <property type="entry name" value="UCP004966"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF126" evidence="3">
        <location>
            <begin position="24"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEF97036.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="129" mass="13922" checksum="3113E105E8DC6C1F" modified="2011-07-27" version="1">MELRGRSISKGIAEGIAIVSKEPFSFLGGVNEKGIIIDKNSELYGESLKDKIFVFPYGKGSTVGSYVIYGLAKRGILKGIVNKECEPIVSTGAILGKIPLVDKVDIEKIKNGDRIVVDGNRGIVIIKEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAX0</accession>
    <name>F6BAX0_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Glycosyl transferase group 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_1523</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97057.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799651.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1523"/>
    <dbReference type="EnsemblBacteria" id="AEF97057">
        <property type="protein sequence ID" value="AEF97057"/>
        <property type="gene ID" value="Metig_1523"/>
    </dbReference>
    <dbReference type="GeneID" id="10644396"/>
    <dbReference type="KEGG" id="mig:Metig_1523"/>
    <dbReference type="HOGENOM" id="CLU_009583_27_5_2"/>
    <dbReference type="OMA" id="VKFLDYV"/>
    <dbReference type="OrthoDB" id="83551at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028098">
        <property type="entry name" value="Glyco_trans_4-like_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13439">
        <property type="entry name" value="Glyco_transf_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glyco_trans_4-like_N" evidence="2">
        <location>
            <begin position="16"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="domain" description="Glycos_transf_1" evidence="1">
        <location>
            <begin position="185"/>
            <end position="348"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF97057.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="374" mass="43882" checksum="7CC88CFD2565ECD0" modified="2011-07-27" version="1">MKIGIISDRLNRPLTGIGNYVYNLINELSKIDKGRIYLINYKENNLFPELNKIIIKNPFEKFPKKPFYFWHLYLNYYLNRKKLDLDIIHSPENIPIFVKLKYQKKIITVHDIIPYIFPKTSIKLNYYRYKYIFPLILKTTDKIITDSYHTKKDLIKHFKIPEDKIKVIHLAANENYKPLKEEEINKIKRKYNLNYPVILYVGALSANKNIPTLLKAFYKLKKQGIKHKLVITGEKRWKYKSIFETIEKLNLQKDVIFTGYVPDEDLPALYNAADLFVYPSLYEGFGLPPLEAMACGTPVITSNTSSLPEVVGDAGIMVNPYDVDELANKMYNVLTNDGLREELSKKGLERAKLFSWKKCAEEHLKVYEEVYNMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAY0</accession>
    <name>F6BAY0_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ABC-type transporter, periplasmic subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1533</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97067.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799661.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1533"/>
    <dbReference type="EnsemblBacteria" id="AEF97067">
        <property type="protein sequence ID" value="AEF97067"/>
        <property type="gene ID" value="Metig_1533"/>
    </dbReference>
    <dbReference type="GeneID" id="10644406"/>
    <dbReference type="KEGG" id="mig:Metig_1533"/>
    <dbReference type="HOGENOM" id="CLU_038034_13_1_2"/>
    <dbReference type="OMA" id="WLMPEYA"/>
    <dbReference type="OrthoDB" id="90656at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002491">
        <property type="entry name" value="ABC_transptr_periplasmic_BD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01497">
        <property type="entry name" value="Peripla_BP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50983">
        <property type="entry name" value="FE_B12_PBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fe/B12 periplasmic-binding" evidence="1">
        <location>
            <begin position="68"/>
            <end position="343"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF97067.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="382" mass="42635" checksum="9994F10D3C02F6B9" modified="2011-07-27" version="1">MLKKIIIISLIVAVASIGLCGCMDKTGINSNITTQASTGKTSLSENTEKIKITDMLGREVEVPKEVNRIVAIGPGCLRLITYLNATDKVVGVEDSEKKWSIYGRPYRIAHPEFANLPTIGKAGPNPKPYPEEIIKVNPDVIFACYITKEQADDLQQKTGIPVVVLSYGRLATFNNKDLFKSIELAGKILGKEERAEEVIKFIKDCLTDLNNRTSDIPDSKKPKVYVGGIGFKGMHGIESTSCNYPPFMAVNAKNVVDELNQSSHVFINKEQLLKWDPDIIFIDEGGLKLVIEDYKKNPDYYNSLKAFKNGNVYGLLPYNFYTTNVGTALADAYFIGKVIYPDRFEDIDPEKKADEIYTFLVGKPVYSVMKEKLGGFKKLEFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAZ3</accession>
    <name>F6BAZ3_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 4">30S ribosomal protein S12</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">rps12</name>
        <name evidence="5" type="ordered locus">Metig_1546</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 4">With S4 and S5 plays an important role in translational accuracy. Located at the interface of the 30S and 50S subunits.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 4">Part of the 30S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 2 3">Belongs to the universal ribosomal protein uS12 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97080.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799674.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1546"/>
    <dbReference type="EnsemblBacteria" id="AEF97080">
        <property type="protein sequence ID" value="AEF97080"/>
        <property type="gene ID" value="Metig_1546"/>
    </dbReference>
    <dbReference type="GeneID" id="10644419"/>
    <dbReference type="KEGG" id="mig:Metig_1546"/>
    <dbReference type="HOGENOM" id="CLU_115574_0_1_2"/>
    <dbReference type="OMA" id="KFRWSQR"/>
    <dbReference type="OrthoDB" id="85293at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03367">
        <property type="entry name" value="Ribosomal_S23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00403_A">
        <property type="entry name" value="Ribosomal_S12_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006032">
        <property type="entry name" value="Ribosomal_S12/S23"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022863">
        <property type="entry name" value="Ribosomal_S12_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005680">
        <property type="entry name" value="Ribosomal_S23_euk/arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11652">
        <property type="entry name" value="PTHR11652"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00164">
        <property type="entry name" value="Ribosom_S12_S23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002133">
        <property type="entry name" value="Ribosomal_S12/S23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00982">
        <property type="entry name" value="uS12_E_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00055">
        <property type="entry name" value="RIBOSOMAL_S12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 3 5" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="2 4" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2 4" id="KW-0699">rRNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU004490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEF97080.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="148" mass="16757" checksum="EA18BDB6784D5E85" modified="2011-07-27" version="1">MSGSKSPRGEFAGRKLRLKRKKCRWHDYNYVRRVLRLKEKYDPLEGAPMARGIVIEKVGLEAKQPNSAIRKCVRVQLIKNGRVVTAFCPGNHAINFIDEHDEVIIEGIGGPKGPRAKGDIPGVKYKVIMVGKNSLRELVRGRQEKVKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-06-02" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>F6BB01</accession>
    <name>F6BB01_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_1554</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97088.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799682.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF97088">
        <property type="protein sequence ID" value="AEF97088"/>
        <property type="gene ID" value="Metig_1554"/>
    </dbReference>
    <dbReference type="GeneID" id="10644427"/>
    <dbReference type="KEGG" id="mig:Metig_1554"/>
    <dbReference type="HOGENOM" id="CLU_2645928_0_0_2"/>
    <dbReference type="OMA" id="IDFEMTY"/>
    <dbReference type="OrthoDB" id="118687at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF97088.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="74" mass="8424" checksum="4433D176CA541598" modified="2011-07-27" version="1">MKVRFKMNIPSKEEICESLKVDNTSEGIIIKVNCKQNNMEVYIETHSIGSLKNALDDLFVNYSMGESIVKIARD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>F6BB05</accession>
    <name>F6BB05_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Metig_1558</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97092.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799686.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1558"/>
    <dbReference type="EnsemblBacteria" id="AEF97092">
        <property type="protein sequence ID" value="AEF97092"/>
        <property type="gene ID" value="Metig_1558"/>
    </dbReference>
    <dbReference type="GeneID" id="10644431"/>
    <dbReference type="KEGG" id="mig:Metig_1558"/>
    <dbReference type="HOGENOM" id="CLU_000604_8_0_2"/>
    <dbReference type="OMA" id="FYRHQTQ"/>
    <dbReference type="OrthoDB" id="45576at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003838">
        <property type="entry name" value="ABC3_permease_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025857">
        <property type="entry name" value="MacB_PCD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02687">
        <property type="entry name" value="FtsX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12704">
        <property type="entry name" value="MacB_PCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="21"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="263"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="300"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="323"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="357"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="domain" description="MacB_PCD" evidence="8">
        <location>
            <begin position="21"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="domain" description="FtsX" evidence="7">
        <location>
            <begin position="295"/>
            <end position="389"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF12704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AEF97092.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="396" mass="43308" checksum="35465EC226E04086" modified="2011-07-27" version="1">MKFIDIIAFAGKNLTKKKTQSILTIIGIVIGITAMVSLISLGYGVQNYIQEETSKLGANKIQILPIKHFGVPPMHYFKYKDVKAVMDVKGVDKVLYGWYGGMNVKFGREERFANLFYAEPSALKSVYEDVGGYGIEKGRWLSDGDKYKCVVGYGVANNLFSKKLKVGDRIVINGKRFRIVGIMNQVGNPQDDNIIIIPLKVGEELLNKKGEYNYMMVKVKKGEDVKKVVEDIKNALKKSIGNDDFSVLTSDQLAKSLGGILDVLTLFVVGIAGISLLVGAVGISNTMYMSILERRKDIGILKALGAETTTILAIFMVESGFLGLFGGIIGLVIGLIIAKIIEIAAKNMGYGMVKAWISWELIVGVLLFSFIVGIVSGFFPARKGAKLDPIETLRGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>F6BB24</accession>
    <name>F6BB24_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Phosphoglucosamine mutase</fullName>
            <ecNumber evidence="5">5.4.2.10</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">glmM</name>
        <name evidence="11" type="ordered locus">Metig_0353</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the conversion of glucosamine-6-phosphate to glucosamine-1-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>alpha-D-glucosamine 1-phosphate = D-glucosamine 6-phosphate</text>
            <dbReference type="Rhea" id="RHEA:23424"/>
            <dbReference type="ChEBI" id="CHEBI:58516"/>
            <dbReference type="ChEBI" id="CHEBI:58725"/>
            <dbReference type="EC" id="5.4.2.10"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="5">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="PTM">
        <text evidence="5">Activated by phosphorylation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5 6">Belongs to the phosphohexose mutase family.</text>
    </comment>
    <dbReference type="EC" id="5.4.2.10" evidence="5"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95909.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798518.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0353"/>
    <dbReference type="EnsemblBacteria" id="AEF95909">
        <property type="protein sequence ID" value="AEF95909"/>
        <property type="gene ID" value="Metig_0353"/>
    </dbReference>
    <dbReference type="GeneID" id="10643190"/>
    <dbReference type="KEGG" id="mig:Metig_0353"/>
    <dbReference type="HOGENOM" id="CLU_016950_7_1_2"/>
    <dbReference type="OMA" id="KCSQVMY"/>
    <dbReference type="OrthoDB" id="58941at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008966">
        <property type="term" value="F:phosphoglucosamine mutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03087">
        <property type="entry name" value="PGM_like1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01554_A">
        <property type="entry name" value="GlmM_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005844">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016055">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-I/II/III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005845">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005846">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005843">
        <property type="entry name" value="A-D-PHexomutase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036900">
        <property type="entry name" value="A-D-PHexomutase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016066">
        <property type="entry name" value="A-D-PHexomutase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005841">
        <property type="entry name" value="Alpha-D-phosphohexomutase_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023666">
        <property type="entry name" value="GlmM_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024086">
        <property type="entry name" value="GlmM_arc-type"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02878">
        <property type="entry name" value="PGM_PMM_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02879">
        <property type="entry name" value="PGM_PMM_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02880">
        <property type="entry name" value="PGM_PMM_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00408">
        <property type="entry name" value="PGM_PMM_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00509">
        <property type="entry name" value="PGMPMM"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53738">
        <property type="entry name" value="SSF53738"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55957">
        <property type="entry name" value="SSF55957"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03990">
        <property type="entry name" value="Arch_GlmM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00710">
        <property type="entry name" value="PGM_PMM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5 11" id="KW-0413">Isomerase</keyword>
    <keyword evidence="3 5 6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2 5 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PGM_PMM_I" evidence="8">
        <location>
            <begin position="2"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="domain" description="PGM_PMM_II" evidence="9">
        <location>
            <begin position="144"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="domain" description="PGM_PMM_III" evidence="10">
        <location>
            <begin position="250"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="domain" description="PGM_PMM_IV" evidence="7">
        <location>
            <begin position="367"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="active site" description="Phosphoserine intermediate" evidence="5">
        <location>
            <position position="88"/>
        </location>
    </feature>
    <feature type="binding site" description="via phosphate group" evidence="5">
        <location>
            <position position="88"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="231"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="233"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="235"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="Phosphoserine" evidence="5">
        <location>
            <position position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AEF95909.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="454" mass="50636" checksum="F2C0EF4B903A1000" modified="2011-07-27" version="1">MKLFGTSGIRMKNLDPEIAYKVGFALSKKVNNVVIGRDTRTTGKLIESALIAGLLNGGSEVVTIGMVPTPVLGFSAREYDAGVMITASHNPPEYNGIKIFNNDGTAYRPSQEEEIENIVFSNGFKRASWDCVGDVWEDKRALKKYMDFILKNVNIETKFNVVVDCCNASGSLVSPYLFTDVGCHVISVNAHVDGRFIGRMPEPNEKNLKNTMAMIKGLNNKDGNYIGIAHDGDADRMIAIDEKGRMTDFDKLLAAFSRYIVEKTGKKIIITTVDASMVIDEYLEDLNVKVIRTKVGDVAVSEAMQKYNALFGGEPSGTWIHGDIHLTPDGILSGLRILEMMEFYGKKLYEIMDEIPSYINLREKVSCEDEKKKKVMEYVIEKGESLFKTTPETVDGARFNVEGGWVLIRPSGTEPYVRVRVEAKDNKTAKELLEKGIKLVNEGLKLGSVQYEYW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>F6BB31</accession>
    <name>F6BB31_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Coenzyme F420 hydrogenase</fullName>
            <ecNumber evidence="5">1.12.98.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Metig_0360</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.12.98.1" evidence="5"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95916.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798525.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0360"/>
    <dbReference type="EnsemblBacteria" id="AEF95916">
        <property type="protein sequence ID" value="AEF95916"/>
        <property type="gene ID" value="Metig_0360"/>
    </dbReference>
    <dbReference type="GeneID" id="10643197"/>
    <dbReference type="KEGG" id="mig:Metig_0360"/>
    <dbReference type="HOGENOM" id="CLU_037958_0_0_2"/>
    <dbReference type="OrthoDB" id="49654at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050454">
        <property type="term" value="F:coenzyme F420 hydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007516">
        <property type="entry name" value="Co_F420_Hydgase/DH_bsu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045220">
        <property type="entry name" value="FRHB/FDHB/HCAR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007525">
        <property type="entry name" value="FrhB_FdhB_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31332">
        <property type="entry name" value="PTHR31332"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04432">
        <property type="entry name" value="FrhB_FdhB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04422">
        <property type="entry name" value="FrhB_FdhB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0408">Iron</keyword>
    <keyword evidence="2" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FrhB_FdhB_N" evidence="3">
        <location>
            <begin position="11"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="domain" description="FrhB_FdhB_C" evidence="4">
        <location>
            <begin position="80"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF04432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEF95916.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="258" mass="28911" checksum="63D6080DA18ED59C" modified="2011-07-27" version="1">MNILSIKSNYDGQDGGFVTALTTYCLKEGILDAVVVVGANNWKPYPFIATKPEDILKASKSKYSISPNNKLLEYATENYDNVGLVGVPCQILGGNKFDLKMKIGLFCTKNFPYDAIKNIVDGLGIPIDRVEKMDIKKGKFIVKTSEIENLIKKKEMVKEIPIKEIEKFANPACRLCCDFSAEYADISVGSVGSEDGWNTVIIRNNNCKKIIDEMHEKGLVEIKLDVDVNAIQKLENIKKRNQEINKCSAYYAVCPYIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>F6BB40</accession>
    <name>F6BB40_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 11">Translation initiation factor 5A</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10 11">Hypusine-containing protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9 11">eIF-5A</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">eif5a</name>
        <name evidence="13" type="ordered locus">Metig_0369</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 11">Functions by promoting the formation of the first peptide bond.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 11">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the eIF-5A family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95925.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="SMR" id="F6BB40"/>
    <dbReference type="STRING" id="880724.Metig_0369"/>
    <dbReference type="EnsemblBacteria" id="AEF95925">
        <property type="protein sequence ID" value="AEF95925"/>
        <property type="gene ID" value="Metig_0369"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0369"/>
    <dbReference type="HOGENOM" id="CLU_102600_3_0_2"/>
    <dbReference type="OMA" id="QIMDMET"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045901">
        <property type="term" value="P:positive regulation of translational elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045905">
        <property type="term" value="P:positive regulation of translational termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00085">
        <property type="entry name" value="eIF_5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001884">
        <property type="entry name" value="IF5A-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_L2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019769">
        <property type="entry name" value="Trans_elong_IF5A_hypusine_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022847">
        <property type="entry name" value="Transl_elong_IF5A_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020189">
        <property type="entry name" value="Transl_elong_IF5A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013185">
        <property type="entry name" value="Transl_elong_KOW-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11673">
        <property type="entry name" value="PTHR11673"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08207">
        <property type="entry name" value="EFP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01287">
        <property type="entry name" value="eIF-5a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003025">
        <property type="entry name" value="eIF5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01376">
        <property type="entry name" value="eIF-5a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="SSF50104"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00037">
        <property type="entry name" value="eIF_5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00302">
        <property type="entry name" value="IF5A_HYPUSINE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 11" id="KW-0385">Hypusine</keyword>
    <keyword evidence="6 11 13" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="7 11" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="eIF-5a" evidence="12">
        <location>
            <begin position="71"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="modified residue" description="Hypusine" evidence="11">
        <location>
            <position position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00016327"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00032030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00032163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM01376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AEF95925.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="133" mass="14386" checksum="B541D9DE2E566D3E" modified="2011-07-27" version="1">MMAGTKQVNLGSLKKGQYIMIDGVPCEIVDISVSKPGKHGGAKARVTAIGIFEPIKKEYVGPTASKVEVPIIDKRKGQVLALMGDLVQIMDLETYETIELPIPEDMEGLEPGAEVEYIEAVGRYKITRVVGGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>F6BB42</accession>
    <name>F6BB42_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="5">tRNA (Adenine-N(1)-)-methyltransferase</fullName>
            <ecNumber evidence="5">2.1.1.36</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Metig_0371</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.1.1.36" evidence="5"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95927.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0371"/>
    <dbReference type="EnsemblBacteria" id="AEF95927">
        <property type="protein sequence ID" value="AEF95927"/>
        <property type="gene ID" value="Metig_0371"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0371"/>
    <dbReference type="HOGENOM" id="CLU_025402_0_1_2"/>
    <dbReference type="OMA" id="VVYPKDA"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031515">
        <property type="term" value="C:tRNA (m1A) methyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016429">
        <property type="term" value="F:tRNA (adenine-N1-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014816">
        <property type="entry name" value="tRNA_MeTrfase_Gcd14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12133">
        <property type="entry name" value="PTHR12133"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08704">
        <property type="entry name" value="GCD14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017269">
        <property type="entry name" value="GCD14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51620">
        <property type="entry name" value="SAM_TRM61"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 5" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2 5" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="S-adenosyl-L-methionine binding" evidence="4">
        <location>
            <begin position="99"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="120"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="174"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="190"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR017269-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEF95927.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="280" mass="31612" checksum="2C9739A3B4E66BC2" modified="2011-07-27" version="1">MMMKKKMLVDERGKKYLIDKKLERFGNDLGVVDLKGKKEGVIVKSHKGHEFYLVEPTIYDIVKKMKRTVTTLLPKDIGLIITHLGIREGETVVEAGTGSGALTIYLANAVGKTGKVITYEKRPEFAKVARKNLEIAGLVRKNQKIIGLDERDVDEDENSEEEEGLYNVIQKIGDVTKGIEEKDVDVIVLDLPDPWNVVPHAKKALNKARGRIATYVPYIEQAKKTVEALKEHGFFDIHTYECLVREIEISEKGVRPSTRMIGHTGYITVARVKPEEENEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>F6BB60</accession>
    <name>F6BB60_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Phosphoesterase RecJ domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metig_0389</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95945.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798554.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0389"/>
    <dbReference type="EnsemblBacteria" id="AEF95945">
        <property type="protein sequence ID" value="AEF95945"/>
        <property type="gene ID" value="Metig_0389"/>
    </dbReference>
    <dbReference type="GeneID" id="10643226"/>
    <dbReference type="KEGG" id="mig:Metig_0389"/>
    <dbReference type="HOGENOM" id="CLU_018957_0_0_2"/>
    <dbReference type="OMA" id="IGYGPDF"/>
    <dbReference type="OrthoDB" id="1910at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031072">
        <property type="term" value="F:heat shock protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd10719">
        <property type="entry name" value="DnaJ_zf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001667">
        <property type="entry name" value="DDH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038763">
        <property type="entry name" value="DHH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001305">
        <property type="entry name" value="HSP_DnaJ_Cys-rich_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036410">
        <property type="entry name" value="HSP_DnaJ_Cys-rich_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022967">
        <property type="entry name" value="S1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003029">
        <property type="entry name" value="S1_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01368">
        <property type="entry name" value="DHH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00575">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00316">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57938">
        <property type="entry name" value="SSF57938"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64182">
        <property type="entry name" value="SSF64182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50126">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51188">
        <property type="entry name" value="ZF_CR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CR-type" evidence="3">
        <location>
            <begin position="1"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="2">
        <location>
            <begin position="135"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="CR-type" evidence="1">
        <location>
            <begin position="1"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEF95945.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="761" mass="86350" checksum="8CAF903850D10BC5" modified="2011-07-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-06-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>F6BB73</accession>
    <name>F6BB73_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_0402</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95958.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798567.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF95958">
        <property type="protein sequence ID" value="AEF95958"/>
        <property type="gene ID" value="Metig_0402"/>
    </dbReference>
    <dbReference type="GeneID" id="10643239"/>
    <dbReference type="KEGG" id="mig:Metig_0402"/>
    <dbReference type="HOGENOM" id="CLU_2519835_0_0_2"/>
    <dbReference type="OMA" id="MNRERKY"/>
    <dbReference type="OrthoDB" id="116298at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF95958.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="83" mass="9525" checksum="383C058B9991407E" modified="2011-07-27" version="1">MVSEKIKQVLDEVQAQLKRDSKYIELVSTVEYLIELVEPSKRDMFKEALENAETIEDVNELIKALKLQIGAQGAKKYALMQQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBC0</accession>
    <name>F6BBC0_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Cobyrinic acid ac-diamide synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1594</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97127.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799720.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1594"/>
    <dbReference type="EnsemblBacteria" id="AEF97127">
        <property type="protein sequence ID" value="AEF97127"/>
        <property type="gene ID" value="Metig_1594"/>
    </dbReference>
    <dbReference type="GeneID" id="10644467"/>
    <dbReference type="KEGG" id="mig:Metig_1594"/>
    <dbReference type="HOGENOM" id="CLU_082962_0_0_2"/>
    <dbReference type="OMA" id="GCYCAVN"/>
    <dbReference type="OrthoDB" id="72034at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002586">
        <property type="entry name" value="CobQ/CobB/MinD/ParA_Nub-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014433">
        <property type="entry name" value="CooC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01656">
        <property type="entry name" value="CbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005647">
        <property type="entry name" value="CooC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CbiA" evidence="1">
        <location>
            <begin position="4"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF97127.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="252" mass="28397" checksum="7ABC6A3493F41FAC" modified="2011-07-27" version="1">MTKIIAISGKGGTGKTMFSTLLIKALSKKTNSILVIDADPDSNLPETLGVEVEKTVGDIREDLKKLVEENKLPPGITKQDYLEGKVYEIIVETENFDLLVMGRPEGSGCYCSVNNWLRQIIDTLAKSYEYVVIDTEAGLEHLSRRTTQNVDLMIVVTDASKRGIGTAKRIKKLANELEVKFKDIYVVANKVNEENEKIVEEYAKELGLNLIGKLPYNKEIAEYDLKGKPLFDLPDNNEVYRKVEEIVEKWII</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBG4</accession>
    <name>F6BBG4_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Transposase, IS605 OrfB family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Metig_1638</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">In the C-terminal section; belongs to the transposase 35 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97171.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799763.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1638"/>
    <dbReference type="EnsemblBacteria" id="AEF97171">
        <property type="protein sequence ID" value="AEF97171"/>
        <property type="gene ID" value="Metig_1638"/>
    </dbReference>
    <dbReference type="GeneID" id="10644511"/>
    <dbReference type="KEGG" id="mig:Metig_1638"/>
    <dbReference type="HOGENOM" id="CLU_032903_3_4_2"/>
    <dbReference type="OMA" id="RINIKYH"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032196">
        <property type="term" value="P:transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001959">
        <property type="entry name" value="Transposase_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010095">
        <property type="entry name" value="Transposase_IS605_OrfB_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01385">
        <property type="entry name" value="OrfB_IS605"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07282">
        <property type="entry name" value="OrfB_Zn_ribbon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01766">
        <property type="entry name" value="tspaseT_teng_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0815">Transposition</keyword>
    <feature type="domain" description="OrfB_IS605" evidence="5">
        <location>
            <begin position="193"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="domain" description="OrfB_Zn_ribbon" evidence="6">
        <location>
            <begin position="304"/>
            <end position="365"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF07282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEF97171.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="406" mass="48070" checksum="C1D2AC97A66F3AE7" modified="2011-07-27" version="1">MSNKTKSKGTQELSYQVVLSYKVSHNYPLKTFLIECKDKLNECIDMIWNNIEYTKKDKPKLPKSNEFKRNLRNKLLENWSYASHYIDGIIKTAYSILQSWASNYKRGYRTKTKPVVERLFVRVKTTLIKYDKERAEIRITIKPRKEYLTLNIKNEWFFDKVKDLTIGEIILKENEAFLTFKDNLNYSDKDIIVGVDSNLKSLDLFHPIEGWIRIDLTELHRLKEVYDKKIDGLKKLLKKCPLRVLRKINRLFERRRNRVKDFLHKLTTQLSRLFPDAIFVFEDLNKRRMYKNKHFNRRIDRANWNGIIEKISYKAIVILVNPAYTSTTCPICGSKMESQEGQVVCYNCSNSFNRQVVGCFNIFKRGLNVIKRFMGGSGVISERSDENLKTVRFLCPRQGQRSLLRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBK0</accession>
    <name>F6BBK0_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Methanogenesis marker 16 metalloprotein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0453</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96009.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798618.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0453"/>
    <dbReference type="EnsemblBacteria" id="AEF96009">
        <property type="protein sequence ID" value="AEF96009"/>
        <property type="gene ID" value="Metig_0453"/>
    </dbReference>
    <dbReference type="GeneID" id="10643290"/>
    <dbReference type="KEGG" id="mig:Metig_0453"/>
    <dbReference type="HOGENOM" id="CLU_061500_0_0_2"/>
    <dbReference type="OMA" id="GCGEINP"/>
    <dbReference type="OrthoDB" id="22946at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002708">
        <property type="entry name" value="HcyBio"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017677">
        <property type="entry name" value="Methan_mark_16"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01837">
        <property type="entry name" value="HcyBio"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03287">
        <property type="entry name" value="methan_mark_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="292"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="326"/>
            <end position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96009.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="389" mass="43904" checksum="1FAE88E275436F61" modified="2011-07-27" version="1">MDKVVVTIDELKKMIRNNEEDKIDEIDIVTAATCGIMSGTMAIFYIPMNDRFRKAEKVYLNGIRGFPGPCPNEYLGSVDVVVFGTEHNGDYGGGFLFKDLVKGKEVEVKVECDGKIYEREITLDEIPTARMIGTRMAFKNYVAVTNFGDEPIKTIFHRKFLRKGEASFSGCGEINPLQNMHVDEKELVGKKILLNGAEGVILGFGTRYSKEKPNIMISADMHNMDAYYLGGFITSTGPEVFNTIAIPIEVNEKHKEYLKKLDEDIDLPLTNVIGRKIVDVGKYSEVWKDVDLRPHINVYRCRNCDFCIVEELCPTKAVRRVEYLGGRRLPNEDCFGCGVCATSCPYGIYQMKLGSILGIPITCRQSDRERALKLAEELKERIIKGEFKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBK8</accession>
    <name>F6BBK8_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Cobyrinic acid ac-diamide synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0461</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96017.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798626.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0461"/>
    <dbReference type="EnsemblBacteria" id="AEF96017">
        <property type="protein sequence ID" value="AEF96017"/>
        <property type="gene ID" value="Metig_0461"/>
    </dbReference>
    <dbReference type="GeneID" id="10643298"/>
    <dbReference type="KEGG" id="mig:Metig_0461"/>
    <dbReference type="HOGENOM" id="CLU_067767_0_0_2"/>
    <dbReference type="OMA" id="MLVTEVK"/>
    <dbReference type="OrthoDB" id="50469at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002586">
        <property type="entry name" value="CobQ/CobB/MinD/ParA_Nub-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01656">
        <property type="entry name" value="CbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00037">
        <property type="entry name" value="Fer4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="61"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="85"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96017.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="269" mass="30001" checksum="02E5DE95E988D494" modified="2011-07-27" version="1">MKIGIVSGKGGVGKSSISTSLAKLFSRELNIIALDCDVDAPNFNLMFDVKDKKLIEVIYRDLYKINENCTFCGKCQDICKFGAIEEYKINPILCEGCGACELICEYNAIEPINHESGYIYEGNCGFPLIWGELEPGESGSGKIIEHIKRHAKQYKSELEIIDGPPGVGCPLFATVKDIDLALCIVEPTKSSVNDCLRLIETLRFFGIDYLIVENKKGMNSIDYPFKIFHSIPYDFEVPKLIANKILLCDGNGKASKAVKELYEKLKEFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-09-29" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBR0</accession>
    <name>F6BBR0_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Cell division ATPase MinD</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metig_1658</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97190.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799781.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF97190">
        <property type="protein sequence ID" value="AEF97190"/>
        <property type="gene ID" value="Metig_1658"/>
    </dbReference>
    <dbReference type="GeneID" id="10644531"/>
    <dbReference type="KEGG" id="mig:Metig_1658"/>
    <dbReference type="HOGENOM" id="CLU_037612_0_3_2"/>
    <dbReference type="OMA" id="KDMAIHL"/>
    <dbReference type="OrthoDB" id="105856at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025669">
        <property type="entry name" value="AAA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010224">
        <property type="entry name" value="MinD_archaea"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025501">
        <property type="entry name" value="MinD_FleN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13614">
        <property type="entry name" value="AAA_31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003092">
        <property type="entry name" value="MinD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01969">
        <property type="entry name" value="minD_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4" id="KW-0132">Cell division</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA_31" evidence="3">
        <location>
            <begin position="2"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEF97190.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="263" mass="29089" checksum="B83F8F69B934D877" modified="2011-07-27" version="1">MIITIASGKGGVGKTTTSANLSVALSKLGKRVLVIDGDISMANLGLIFNFEKKKPNLHDVLAGEAYVKDAIYKHKTGVYVLPTSLSIEGYKKSDLDLFPEVVNEVADEYDYVIIDAPAGLNRDMAMHLAIADKLMLIVTPELFSIVDGLRIKESAEMAGTPLMGIVLNRTGRDFAEMGEDEIEMLIKGRIIGNIPEDENIRTATLKKMTVLEYSPNSPSSKAYMELALRVVGSYVDIEKIEKMHKESFLQKIKRKITNVFSRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBR9</accession>
    <name>F6BBR9_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="4">TrkA-N domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metig_1667</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97199.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799790.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1667"/>
    <dbReference type="EnsemblBacteria" id="AEF97199">
        <property type="protein sequence ID" value="AEF97199"/>
        <property type="gene ID" value="Metig_1667"/>
    </dbReference>
    <dbReference type="GeneID" id="10644540"/>
    <dbReference type="KEGG" id="mig:Metig_1667"/>
    <dbReference type="HOGENOM" id="CLU_050982_0_1_2"/>
    <dbReference type="OMA" id="HIIVCGC"/>
    <dbReference type="OrthoDB" id="80000at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008324">
        <property type="term" value="F:cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006813">
        <property type="term" value="P:potassium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013099">
        <property type="entry name" value="K_chnl_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006037">
        <property type="entry name" value="RCK_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036721">
        <property type="entry name" value="RCK_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003148">
        <property type="entry name" value="RCK_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07885">
        <property type="entry name" value="Ion_trans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02080">
        <property type="entry name" value="TrkA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02254">
        <property type="entry name" value="TrkA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF116726">
        <property type="entry name" value="SSF116726"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51202">
        <property type="entry name" value="RCK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51201">
        <property type="entry name" value="RCK_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="32"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="64"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="domain" description="RCK N-terminal" evidence="2">
        <location>
            <begin position="109"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="domain" description="RCK C-terminal" evidence="3">
        <location>
            <begin position="252"/>
            <end position="337"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEF97199.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="338" mass="38111" checksum="FF1505D987E4EB0A" modified="2011-07-27" version="1">METSRKIVYTIILVFLLIFSYSFAIMKIEGLPFLDALYFSIITITTTGYGDYTPTTYEGRILTIIYLFFGIGIVMYLFGIIAQFIIEGEFKNLVRMRKMENRIKELKDHYIICGFGRIGRIVANKFKKEKIPFIVIDINKEILKEELNKDPNFNFIVGDARKDETLKKAKIEKAKGLITTLPTDADNVFITLSAKGLNPKIKVVAKADEEEAIKKLKRAGADKVVSPYMIGGLRLAEVALRPGILDFVSTFINIAKYEYGEDVELRKFIVEKGSEIDGKSLYESDIRKKVGLTILGIKKGDNLIINPPSDIIINAGDEIYAFGSGSQLKSLEKMVKNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBT8</accession>
    <name>F6BBT8_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein-export membrane protein SecD</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">secD</name>
        <name evidence="10" type="ordered locus">Metig_1686</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Involved in protein export.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Part of the protein translocation apparatus. Forms a complex with SecF.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cell membrane</location>
            <topology evidence="9">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the SecD/SecF family. SecD subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97218.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799808.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1686"/>
    <dbReference type="EnsemblBacteria" id="AEF97218">
        <property type="protein sequence ID" value="AEF97218"/>
        <property type="gene ID" value="Metig_1686"/>
    </dbReference>
    <dbReference type="GeneID" id="10644559"/>
    <dbReference type="KEGG" id="mig:Metig_1686"/>
    <dbReference type="HOGENOM" id="CLU_007894_5_1_2"/>
    <dbReference type="OMA" id="SAHLQMM"/>
    <dbReference type="OrthoDB" id="14000at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0065002">
        <property type="term" value="P:intracellular protein transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006605">
        <property type="term" value="P:protein targeting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01463_A">
        <property type="entry name" value="SecD_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022813">
        <property type="entry name" value="SecD/SecF_arch_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022646">
        <property type="entry name" value="SecD/SecF_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024912">
        <property type="entry name" value="SecD_arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30081">
        <property type="entry name" value="PTHR30081"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07549">
        <property type="entry name" value="Sec_GG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02355">
        <property type="entry name" value="SecD_SecF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 9" id="KW-0811">Translocation</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="239"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="267"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="295"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="334"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="362"/>
            <end position="381"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AEF97218.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="389" mass="41892" checksum="AD15032FA673801D" modified="2011-07-27" version="1">MKLLKDSKILILLISLVASILLIAFKGVSLGIDLSGGSIIVLKSERPMTDEEITLTVDILGKRLNANGLSDVRIYPRGNDEIIVEIPKSADLERIEKILSKQGVFVAKIDNITAYTGKDVKSVEIPQKIPQGNSWAYGVPFELTLEGAKKFAEVAKGKAYHRVELYMDGKLISAPVLAEELTDGKPHPRQVITVGPYPPTKEEEDEAMAIYTALKSGSLPVKVEIEYVSSISPTLGKEFVKGALIAGLLAFIAVGLVVGVRYKQPKIVLPILFTCASEILLILGFASLIDWKIDLASIAGIIAAVGTGVDDQIVITDEVLKGTKKLKKSIKRAFFIIFAAAGTTIAAMTPLFVMGVGMLKGFAITTIAGVLIGVFITRPAFARIIKYIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBU2</accession>
    <name>F6BBU2_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">tRNA-t(6)A37 methylthiotransferase</fullName>
            <ecNumber evidence="9">2.8.4.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">Metig_1690</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 9">Catalyzes the methylthiolation of N6-threonylcarbamoyladenosine (t(6)A), leading to the formation of 2-methylthio-N6-threonylcarbamoyladenosine (ms(2)t(6)A) at position 37 in tRNAs that read codons beginning with adenine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>[sulfur carrier]-SH + AH2 + N(6)-L-threonylcarbamoyladenosine(37) in tRNA + 2 S-adenosyl-L-methionine = 2-methylsulfanyl-N(6)-L-threonylcarbamoyladenosine(37) in tRNA + 5'-deoxyadenosine + [sulfur carrier]-H + A + 2 H(+) + L-methionine + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:37075"/>
            <dbReference type="Rhea" id="RHEA-COMP:10163"/>
            <dbReference type="Rhea" id="RHEA-COMP:11092"/>
            <dbReference type="Rhea" id="RHEA-COMP:14737"/>
            <dbReference type="Rhea" id="RHEA-COMP:14739"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:29917"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:64428"/>
            <dbReference type="ChEBI" id="CHEBI:74418"/>
            <dbReference type="ChEBI" id="CHEBI:74420"/>
            <dbReference type="EC" id="2.8.4.5"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="9">Binds 1 or 2 [4Fe-4S] cluster. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the methylthiotransferase family. CDKAL1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.8.4.5" evidence="9"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97222.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799812.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1690"/>
    <dbReference type="EnsemblBacteria" id="AEF97222">
        <property type="protein sequence ID" value="AEF97222"/>
        <property type="gene ID" value="Metig_1690"/>
    </dbReference>
    <dbReference type="GeneID" id="10644563"/>
    <dbReference type="KEGG" id="mig:Metig_1690"/>
    <dbReference type="HOGENOM" id="CLU_018697_4_2_2"/>
    <dbReference type="OMA" id="CEHFHIP"/>
    <dbReference type="OrthoDB" id="18705at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035598">
        <property type="term" value="F:N6-threonylcarbomyladenosine methylthiotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061712">
        <property type="term" value="F:tRNA (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-methylthiotransferase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.30.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaB/NifB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005839">
        <property type="entry name" value="Methylthiotransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020612">
        <property type="entry name" value="Methylthiotransferase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013848">
        <property type="entry name" value="Methylthiotransferase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038135">
        <property type="entry name" value="Methylthiotransferase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006466">
        <property type="entry name" value="MiaB-like_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023404">
        <property type="entry name" value="rSAM_horseshoe"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002792">
        <property type="entry name" value="TRAM_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01938">
        <property type="entry name" value="TRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00919">
        <property type="entry name" value="UPF0004"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01061">
        <property type="entry name" value="methylthiotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01578">
        <property type="entry name" value="MiaB-like-B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00089">
        <property type="entry name" value="TIGR00089"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51449">
        <property type="entry name" value="MTTASE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01278">
        <property type="entry name" value="MTTASE_RADICAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50926">
        <property type="entry name" value="TRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="7 9" id="KW-0408">Iron</keyword>
    <keyword evidence="8 9" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="6 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="MTTase N-terminal" evidence="11">
        <location>
            <begin position="1"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="domain" description="Radical SAM core" evidence="12">
        <location>
            <begin position="127"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="domain" description="TRAM" evidence="10">
        <location>
            <begin position="359"/>
            <end position="413"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU368081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AEF97222.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="413" mass="47308" checksum="24DFBFFD24936AB9" modified="2011-07-27" version="1">MKVYVEGYGCTLNTADTNIIENSLRDYGFEITSNIEDADLVIINTCVVRLETENRMFARINYFKSLNKKVVVAGCLAKALKKKVENLADLLIMPREAHLSGKILYKHFIEKSSIEKDINLEEKLKYLSPSLITPLPISEGCIGNCSYCIVKIARGRLVSYSREKLVEKAKELINSGTKCLLITAQDTACYGFDRNDNLPNLINDLCSIEGEFIMRIGMMHAKNVENILDDLIEAYKDEKVAKLLHLPLQSGDDDVLKAMKREYTVDEFISIVNEFRKKIKDLTFVTDIIVGFPTETEEAFENTLEVLRKLKPDYIHGAKYTSRKYTEAARMKQIDTKIRKRRSEILDKLRRELSYMNNKKYIGKKMRVLVTEEGKGITHNFKVVRFNKNAEIGTFKEIKITDAKTFGLFGELC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBV0</accession>
    <name>F6BBV0_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Precorrin-8X methylmutase CbiC/CobH</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Metig_1698</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; adenosylcobalamin biosynthesis.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97230.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1698"/>
    <dbReference type="EnsemblBacteria" id="AEF97230">
        <property type="protein sequence ID" value="AEF97230"/>
        <property type="gene ID" value="Metig_1698"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_1698"/>
    <dbReference type="HOGENOM" id="CLU_084703_1_0_2"/>
    <dbReference type="OMA" id="GAPIFCD"/>
    <dbReference type="UniPathway" id="UPA00148"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016993">
        <property type="term" value="F:precorrin-8X methylmutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003722">
        <property type="entry name" value="Cbl_synth_CobH/CbiC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036588">
        <property type="entry name" value="CobH/CbiC_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02570">
        <property type="entry name" value="CbiC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63965">
        <property type="entry name" value="SSF63965"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword evidence="3" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CbiC" evidence="4">
        <location>
            <begin position="61"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02570"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEF97230.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="260" mass="28980" checksum="2DDAD38155D31B0F" modified="2011-07-27" version="1">MAIIYLRGTAERSEAVHPILMKSKRSFELQNPSNAHDYGLEVVMLKFHEFMGAITKEGISIAEKSREIVKNKIRNVLGDKINSYSKEELSIIERVVHATADEEYAKLIYFKDNAVEEGVKAIKENKPIVVDINMIKAGIRYNNTYCFINDRKTYELAQKEQITRAVASIRIAKDLIDDGIVVIGNAPTALLEVIRMINEENIKPRVVIGVPVGFVQASESKELLRKTDVPSITTIGPKGGTPVAVSIINGIIAMSRDERV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBX6</accession>
    <name>F6BBX6_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Replication factor C small subunit</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Clamp loader small subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">Metig_1724</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the activator 1 small subunits family. RfcS subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97256.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799845.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1724"/>
    <dbReference type="EnsemblBacteria" id="AEF97256">
        <property type="protein sequence ID" value="AEF97256"/>
        <property type="gene ID" value="Metig_1724"/>
    </dbReference>
    <dbReference type="GeneID" id="10644598"/>
    <dbReference type="KEGG" id="mig:Metig_1724"/>
    <dbReference type="HOGENOM" id="CLU_015698_2_0_2"/>
    <dbReference type="OMA" id="NWRYEFR"/>
    <dbReference type="OrthoDB" id="37207at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016539">
        <property type="term" value="P:intein-mediated protein splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006314">
        <property type="term" value="P:intron homing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.28.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008921">
        <property type="entry name" value="DNA_pol3_clamp-load_cplx_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003586">
        <property type="entry name" value="Hint_dom_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003587">
        <property type="entry name" value="Hint_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036844">
        <property type="entry name" value="Hint_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027434">
        <property type="entry name" value="Homing_endonucl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006142">
        <property type="entry name" value="INTEIN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030934">
        <property type="entry name" value="Intein_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004042">
        <property type="entry name" value="Intein_endonuc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006141">
        <property type="entry name" value="Intein_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004860">
        <property type="entry name" value="LAGLIDADG_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013748">
        <property type="entry name" value="Rep_factorC_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14528">
        <property type="entry name" value="LAGLIDADG_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08542">
        <property type="entry name" value="Rep_fac_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00379">
        <property type="entry name" value="INTEIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00305">
        <property type="entry name" value="HintC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00306">
        <property type="entry name" value="HintN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48019">
        <property type="entry name" value="SSF48019"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51294">
        <property type="entry name" value="SSF51294"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55608">
        <property type="entry name" value="SSF55608"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01443">
        <property type="entry name" value="intein_Cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01445">
        <property type="entry name" value="intein_Nterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50818">
        <property type="entry name" value="INTEIN_C_TER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50819">
        <property type="entry name" value="INTEIN_ENDONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50817">
        <property type="entry name" value="INTEIN_N_TER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0068">Autocatalytic cleavage</keyword>
    <keyword evidence="6" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0404">Intron homing</keyword>
    <keyword evidence="6" id="KW-0540">Nuclease</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0651">Protein splicing</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DOD-type homing endonuclease" evidence="10">
        <location>
            <begin position="230"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="domain" description="INTEIN_C_TER" evidence="9">
        <location>
            <begin position="490"/>
            <end position="514"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00014164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00031749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50818"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AEF97256.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="756" mass="87198" checksum="42404E5CAFA0FF94" modified="2011-07-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBX9</accession>
    <name>F6BBX9_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Aldehyde ferredoxin oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_1727</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AOR/FOR family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97259.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799848.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1727"/>
    <dbReference type="EnsemblBacteria" id="AEF97259">
        <property type="protein sequence ID" value="AEF97259"/>
        <property type="gene ID" value="Metig_1727"/>
    </dbReference>
    <dbReference type="GeneID" id="10644601"/>
    <dbReference type="KEGG" id="mig:Metig_1727"/>
    <dbReference type="HOGENOM" id="CLU_440510_0_0_2"/>
    <dbReference type="OMA" id="CRFHRGW"/>
    <dbReference type="OrthoDB" id="5023at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016625">
        <property type="term" value="F:oxidoreductase activity, acting on the aldehyde or oxo group of donors, iron-sulfur protein as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.569.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.9.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013984">
        <property type="entry name" value="Ald_Fedxn_OxRdtase_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013983">
        <property type="entry name" value="Ald_Fedxn_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036503">
        <property type="entry name" value="Ald_Fedxn_OxRdtase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001203">
        <property type="entry name" value="OxRdtase_Ald_Fedxn_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036021">
        <property type="entry name" value="Tungsten_al_ferr_oxy-like_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01314">
        <property type="entry name" value="AFOR_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02730">
        <property type="entry name" value="AFOR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00790">
        <property type="entry name" value="AFOR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48310">
        <property type="entry name" value="SSF48310"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56228">
        <property type="entry name" value="SSF56228"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AFOR_N" evidence="2">
        <location>
            <begin position="1"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF97259.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="617" mass="71709" checksum="7C5BA54D657E872B" modified="2011-07-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>F6BC14</accession>
    <name>F6BC14_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Radical SAM domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Metig_0541</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the radical SAM superfamily. Anaerobic sulfatase-maturating enzyme family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96095.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798702.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96095">
        <property type="protein sequence ID" value="AEF96095"/>
        <property type="gene ID" value="Metig_0541"/>
    </dbReference>
    <dbReference type="GeneID" id="10643379"/>
    <dbReference type="KEGG" id="mig:Metig_0541"/>
    <dbReference type="HOGENOM" id="CLU_009273_3_2_2"/>
    <dbReference type="OMA" id="CYAREFL"/>
    <dbReference type="OrthoDB" id="18533at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaB/NifB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000385">
        <property type="entry name" value="MoaA_NifB_PqqE_Fe-S-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023867">
        <property type="entry name" value="Sulphatase_maturase_rSAM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43273">
        <property type="entry name" value="PTHR43273"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01384">
        <property type="entry name" value="thioether_bond_formation_requi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01305">
        <property type="entry name" value="MOAA_NIFB_PQQE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="7">
        <location>
            <begin position="1"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEF96095.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="277" mass="32302" checksum="7A68A93F03F3C109" modified="2011-07-27" version="1">MKFLILKITDRCNLNCTYCYSLNKNNKDMEFRIAKKAINYLLNFDDNIKIQFTGGEPLLNFKLIEKVVNYTEDKGNFRYAIQTNGTLLSDYIEEIKELNINVGVSIDGLEVNDVLRPYKEGGSSTLDTLKGIYLLKEYNIPFGVTIVITNKNLPHLEEFVNYLIALGVKSFSFDLLKPKKREDLKLMPNIREFEKVLNNLSKYPVYIKNLYQRKWDYCFLNRGDLLFVNEVGDIYPCPTLEGHFYMGNIENFNGELFKVKSKGCFAREYLYRLNGLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-06-02" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>F6BC20</accession>
    <name>F6BC20_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0547</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96101.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798708.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96101">
        <property type="protein sequence ID" value="AEF96101"/>
        <property type="gene ID" value="Metig_0547"/>
    </dbReference>
    <dbReference type="GeneID" id="10643385"/>
    <dbReference type="KEGG" id="mig:Metig_0547"/>
    <dbReference type="HOGENOM" id="CLU_1472084_0_0_2"/>
    <dbReference type="OMA" id="CGGLECN"/>
    <dbReference type="OrthoDB" id="98952at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008965">
        <property type="entry name" value="CBM2/CBM3_carb-bd_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49384">
        <property type="entry name" value="SSF49384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="157"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96101.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="185" mass="20131" checksum="F69336E15471290D" modified="2011-07-27" version="1">MKKFAALLILLILPTCMAIDISVNAPPKVKKGDLFTVEVYANNVSSCGGLECNIYLSDNLVVDDVITYDVGADYNLTMTMNKNRSAAIQYAFLTNPKNEDFKVGEFKIKAIKSGNATITIKAVASDSEGNAIKIPQKTVNLKITSNSTQKTEKNDSFLFNPITIIFAVLLVGLVAYIIKKVVGNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F6BC43</accession>
    <name>F6BC43_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="7">ABC-type transporter, integral membrane subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Metig_0570</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96124.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798731.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0570"/>
    <dbReference type="EnsemblBacteria" id="AEF96124">
        <property type="protein sequence ID" value="AEF96124"/>
        <property type="gene ID" value="Metig_0570"/>
    </dbReference>
    <dbReference type="GeneID" id="10643408"/>
    <dbReference type="KEGG" id="mig:Metig_0570"/>
    <dbReference type="HOGENOM" id="CLU_031365_1_0_2"/>
    <dbReference type="OMA" id="FGDVAYY"/>
    <dbReference type="OrthoDB" id="49280at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015658">
        <property type="term" value="F:branched-chain amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06581">
        <property type="entry name" value="TM_PBP1_LivM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001851">
        <property type="entry name" value="ABC_transp_permease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043428">
        <property type="entry name" value="LivM-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30482">
        <property type="entry name" value="PTHR30482"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02653">
        <property type="entry name" value="BPD_transp_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="39"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="86"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="124"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="165"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="220"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="245"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="270"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="301"/>
            <end position="319"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEF96124.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="343" mass="37844" checksum="3956B953531F859C" modified="2011-07-27" version="1">MIVDLIQLILIWFGLYLIVNLSLNIEFGYGGIPNFGKHLAVLLGAVVVGGIVNRILMLIFGINGDIVTASTMVSSVVNDLISQNPLYGIGILLLSIFLAIVLGLIIGALFILPSAKLKEDYLGVTLLAIAEVFFLSTYYTPSIIGGYYGASVPDILAFVSGEMRGFVFAGLILLIAFVIYLFTNKLLNTPYGRILRAMRENENVVIAFGRDIMKIRIKTVSIGTAIAAIAGVLFGFYSANVIGTAFTRVEWTFFPFLMVLLGGKGNNKGIILGTLIFVITKVLLETYKFEIKEFLHLPFEAVWLEYIIFGILVVLILYYRPEGILSEKPILTKPIKKIMHNQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>F6BC77</accession>
    <name>F6BC77_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_1751</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97283.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799872.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1751"/>
    <dbReference type="EnsemblBacteria" id="AEF97283">
        <property type="protein sequence ID" value="AEF97283"/>
        <property type="gene ID" value="Metig_1751"/>
    </dbReference>
    <dbReference type="GeneID" id="10644625"/>
    <dbReference type="KEGG" id="mig:Metig_1751"/>
    <dbReference type="HOGENOM" id="CLU_077587_0_0_2"/>
    <dbReference type="OMA" id="PGGWVIG"/>
    <dbReference type="OrthoDB" id="66950at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF97283.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="306" mass="35929" checksum="467FCCD8E704CB2D" modified="2011-07-27" version="1">MNHQNKKEIINELHKKIKEIRRDLNLEDVPLEIVDVEVKKENGKTSLIIYTLTRSDKSTIIGPGGWVVGKLREELKDRFDSITVEDYTDVLVYKKRLEERLRFFENNNLEFLKDVVHYLIKNEIPLKKEKVMVLVQCKHDLAILDILNKVYDVHAITYDGGGVVLPPKTKQKIEEFIKSKNIKHEYIKEKLSREKTLNLIDNYPCGFIKDIIKEKAKLCNIKYVFLTCLDGDYKIEDDIYFINFLKMFPIRLKKDNYLDFCPLLIQSYKSKNSNKIKIIDEIVSDVYNGLKEPTEATEEILKVVRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCC1</accession>
    <name>F6BCC1_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Coenzyme F420 hydrogenase, subunit alpha</fullName>
            <ecNumber evidence="2">1.12.98.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1795</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.12.98.1" evidence="2"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97327.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1795"/>
    <dbReference type="EnsemblBacteria" id="AEF97327">
        <property type="protein sequence ID" value="AEF97327"/>
        <property type="gene ID" value="Metig_1795"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_1795"/>
    <dbReference type="HOGENOM" id="CLU_044556_1_0_2"/>
    <dbReference type="OMA" id="HGIASCE"/>
    <dbReference type="OrthoDB" id="32760at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050454">
        <property type="term" value="F:coenzyme F420 hydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008901">
        <property type="term" value="F:ferredoxin hydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016151">
        <property type="term" value="F:nickel cation binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.645.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017682">
        <property type="entry name" value="Coenz_F420_hydrogenase_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001501">
        <property type="entry name" value="Ni-dep_hyd_lsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018194">
        <property type="entry name" value="Ni-dep_hyd_lsu_Ni_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029014">
        <property type="entry name" value="NiFe-Hase_large"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00374">
        <property type="entry name" value="NiFeSe_Hases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56762">
        <property type="entry name" value="SSF56762"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03295">
        <property type="entry name" value="frhA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00507">
        <property type="entry name" value="NI_HGENASE_L_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF97327.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="385" mass="42964" checksum="D718BE3F6C7A3BD6" modified="2011-07-27" version="1">MTNTIEIAPTTRHEGHAKLILEVNDAGIVTKAYYPNTTPVRGFETMLKGRPAEFAPIAVMRICGICQTTHGIASCEAIEDAIGCEIPEDGRLLRELVGLGNRMHSHPLHHLLIADDFIKPNEQDLKVEAIKLIQKMRKVGQLIVDIVGGEGIHPPNIVIGGMRTNITERAKSKIYYACREYEKAAYQMYELLEMLIERYLEEVGISDLGAHDYPYIATHTTFGDRNAIDWDNVTEIPAQRYYKNPEIAQTATNQIPLYCGVPVEGGPRARMVKFGNFKAGGSALDINIARAQENLGAVYRVFEILDELNLNGKTRVEPEYKDGFGIGVHEAPRATNVHMAEVGKDGRIKSYKIIAASTWNMPIVEKAIEGYPHQYAEVIMRAYDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCD2</accession>
    <name>F6BCD2_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_0589</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96143.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798750.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0589"/>
    <dbReference type="EnsemblBacteria" id="AEF96143">
        <property type="protein sequence ID" value="AEF96143"/>
        <property type="gene ID" value="Metig_0589"/>
    </dbReference>
    <dbReference type="GeneID" id="10643427"/>
    <dbReference type="KEGG" id="mig:Metig_0589"/>
    <dbReference type="HOGENOM" id="CLU_131306_1_1_2"/>
    <dbReference type="OMA" id="RTFCYAT"/>
    <dbReference type="OrthoDB" id="104786at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1440.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022803">
        <property type="entry name" value="Ribosomal_L5_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002739">
        <property type="entry name" value="UPF0201"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01877">
        <property type="entry name" value="RNA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55282">
        <property type="entry name" value="SSF55282"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF96143.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="149" mass="17334" checksum="54DEDC6D64B890AF" modified="2011-07-27" version="1">MVNYIIITATAHATEDEDKVLDAMAYFLPDVVGEDDVELEVVETDGYFGNPINVYKMTINKKANKVFKHIMDLLKSDERNINKLKKDIDLRIEKNKLYLRFDKQKAYLGECKLMDGDDVVRIVINFKVYVSKNKEEKVKERVLKELGIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCE2</accession>
    <name>F6BCE2_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="6">ATPase associated with various cellular activities AAA_5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Metig_0599</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Mg-chelatase subunits D/I family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96153.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0599"/>
    <dbReference type="EnsemblBacteria" id="AEF96153">
        <property type="protein sequence ID" value="AEF96153"/>
        <property type="gene ID" value="Metig_0599"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0599"/>
    <dbReference type="HOGENOM" id="CLU_016684_0_2_2"/>
    <dbReference type="OMA" id="RISAVCG"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045006">
        <property type="entry name" value="CHLI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041628">
        <property type="entry name" value="ChlI/MoxR_AAA_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000523">
        <property type="entry name" value="Mg_chelatse_chII-like_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32039">
        <property type="entry name" value="PTHR32039"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17863">
        <property type="entry name" value="AAA_lid_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01078">
        <property type="entry name" value="Mg_chelatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA" evidence="5">
        <location>
            <begin position="27"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="333"/>
            <end position="359"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEF96153.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="359" mass="40882" checksum="4C7A0A115E11D41C" modified="2011-07-27" version="1">MMNYPFTAIVGQEKMKKALILNAINPKIGGVLIRGEKGTAKSTTVRALADLLPEIEVVEGCPFNCDPNGDLCDICREKKERGELKTIKKKMKVINLPIGATEDRVIGTLDIEKAIKEGIKALDPGILAEANRNILYIDEVNLLDDHIVDILLDAAAMGWNIIEREGIKIKHPSRFILVGTMNPEEGELRPQILDRFGLMVDVEGLNDIKQRVEVIKRVEEFNENPEEFYKKYEEEQLKLREKIEKARELLKNVKISDDLLELISKICIELGIPTNRADITVARTAKTIAAFNGRDYVTVDDVKEACELALPHRMRRKPFEPPQLNKEKIEQIFNEFEEKNNEKNEDEKGNKENDKVKKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCF4</accession>
    <name>F6BCF4_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Zn_Tnp_IS1595 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0614</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96165.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0614"/>
    <dbReference type="EnsemblBacteria" id="AEF96165">
        <property type="protein sequence ID" value="AEF96165"/>
        <property type="gene ID" value="Metig_0614"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0614"/>
    <dbReference type="HOGENOM" id="CLU_2011585_0_0_2"/>
    <dbReference type="OMA" id="RQCISRI"/>
    <dbReference type="OrthoDB" id="97396at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024442">
        <property type="entry name" value="Transposase_Zn_ribbon"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12760">
        <property type="entry name" value="Zn_Tnp_IS1595"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zn_Tnp_IS1595" evidence="1">
        <location>
            <begin position="12"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96165.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="133" mass="15805" checksum="B281839AB093949F" modified="2011-07-27" version="1">MSVEVYKLFIPKDEECIEVIRRVRWGNGYICPYCGSKDVVLKGKERRKPYIQRYRCNSCKRHFNDLTGTIFAKKRITLGEMFYIAKNLGNSSINQMSKELGRDYRTVHSFAKEIMELSEKDNLLKKLLEKNWR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-06-02" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCF5</accession>
    <name>F6BCF5_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transposase IS4 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0615</name>
        <name evidence="3" type="ordered locus">Metig_1645</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96166.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97177.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798772.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96166">
        <property type="protein sequence ID" value="AEF96166"/>
        <property type="gene ID" value="Metig_0615"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF97177">
        <property type="protein sequence ID" value="AEF97177"/>
        <property type="gene ID" value="Metig_1645"/>
    </dbReference>
    <dbReference type="GeneID" id="10644518"/>
    <dbReference type="KEGG" id="mig:Metig_0615"/>
    <dbReference type="KEGG" id="mig:Metig_1645"/>
    <dbReference type="HOGENOM" id="CLU_071602_0_0_2"/>
    <dbReference type="OMA" id="NTEAARC"/>
    <dbReference type="OrthoDB" id="42868at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:transposition, DNA-mediated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002559">
        <property type="entry name" value="Transposase_11"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01609">
        <property type="entry name" value="DDE_Tnp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DDE_Tnp_1" evidence="1">
        <location>
            <begin position="97"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96166.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF97177.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="332" mass="39128" checksum="38DD85B22C123B29" modified="2011-07-27" version="1">MLKKMGSIVYMLFILLSEVNSCVELSKTLRIFGINISHDTINRILWDEGISPQERLFSYVRDFIIPDYNIIVIDDFVIDKIYSKSTEFTYYCWSNLHKRVVKGIHIVDCIVTNGKNIIPIDFRVYDKQRDGKTKNDLCREIISSLVEKGLNIRYICFDSWYSSKENLKLIDKFGLFYLCRIKRNRKIKLSKNGEWISIKELGEIPKSGLIVYLRKVGYVKLFCLSKNGKAVYYITNNLFMDFEEFQEVRNASWRIEEFHRGVKQCCNIGNFFVRKRLPVLGHISLAMRAFFILERIRIDKKITWYEFRRELNRIAVGNAIISLCKEAGLLLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-09-29" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCF7</accession>
    <name>F6BCF7_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ATPase_2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0617</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96168.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798773.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96168">
        <property type="protein sequence ID" value="AEF96168"/>
        <property type="gene ID" value="Metig_0617"/>
    </dbReference>
    <dbReference type="GeneID" id="10643456"/>
    <dbReference type="KEGG" id="mig:Metig_0617"/>
    <dbReference type="HOGENOM" id="CLU_068608_0_0_2"/>
    <dbReference type="OMA" id="REKETHR"/>
    <dbReference type="OrthoDB" id="44502at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011579">
        <property type="entry name" value="ATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01637">
        <property type="entry name" value="ATPase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATPase_2" evidence="1">
        <location>
            <begin position="3"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96168.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="363" mass="43209" checksum="AD00787930DF1B3E" modified="2011-07-27" version="1">MKFFNREKETHRILSIIKGEPRLIHFIYGPLNSGKSTLMREILANRLDKSKYIPFFIDFRTRNILNVDNFIECLFEVDEKSKIDNFREYAKSLADLLVKSSEEVSSYYLGMPIKIPKPFFEKIFNKKDKSGDVYQYVEYLFAKLNEKGKTPILIFDELQMIRKVTLNGNRLLLWSLFQFLVSLTKVQHLCHVFCLSSDSLFIEYVYNIGELENRADYILVDDFDKQTAIKFIDFLAKEYNINLSNKDKELIYSYVGGKPILIEVVIDRLRYEKLGEILNSMLRDEIQKLKYFLEDVKEEDEEFYKKIVDALRIFKEDYEVEDMEIPKKIREFLIKKNILFLNPQKGTLKPQSYLVWKSIKRVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCH1</accession>
    <name>F6BCH1_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Anthranilate synthase component 1</fullName>
            <ecNumber evidence="8">4.1.3.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">trpE</name>
        <name evidence="11" type="ordered locus">Metig_0632</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Part of a heterotetrameric complex that catalyzes the two-step biosynthesis of anthranilate, an intermediate in the biosynthesis of L-tryptophan. In the first step, the glutamine-binding beta subunit (TrpG) of anthranilate synthase (AS) provides the glutamine amidotransferase activity which generates ammonia as a substrate that, along with chorismate, is used in the second step, catalyzed by the large alpha subunit of AS (TrpE) to produce anthranilate. In the absence of TrpG, TrpE can synthesize anthranilate directly from chorismate and high concentrations of ammonia.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>chorismate + L-glutamine = anthranilate + H(+) + L-glutamate + pyruvate</text>
            <dbReference type="Rhea" id="RHEA:21732"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16567"/>
            <dbReference type="ChEBI" id="CHEBI:29748"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="EC" id="4.1.3.27"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 8">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 8">Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 1/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Heterotetramer consisting of two non-identical subunits: a beta subunit (TrpG) and a large alpha subunit (TrpE).</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 8">Belongs to the anthranilate synthase component I family.</text>
    </comment>
    <dbReference type="EC" id="4.1.3.27" evidence="8"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96182.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798787.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0632"/>
    <dbReference type="EnsemblBacteria" id="AEF96182">
        <property type="protein sequence ID" value="AEF96182"/>
        <property type="gene ID" value="Metig_0632"/>
    </dbReference>
    <dbReference type="GeneID" id="10643471"/>
    <dbReference type="KEGG" id="mig:Metig_0632"/>
    <dbReference type="HOGENOM" id="CLU_006493_9_3_2"/>
    <dbReference type="OMA" id="YMYFLEY"/>
    <dbReference type="OrthoDB" id="13784at2157"/>
    <dbReference type="UniPathway" id="UPA00035">
        <property type="reaction ID" value="UER00040"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004049">
        <property type="term" value="F:anthranilate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000162">
        <property type="term" value="P:tryptophan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.120.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005801">
        <property type="entry name" value="ADC_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019999">
        <property type="entry name" value="Anth_synth_I-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006805">
        <property type="entry name" value="Anth_synth_I_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010116">
        <property type="entry name" value="Anthranilate_synth_I_arc_typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015890">
        <property type="entry name" value="Chorismate_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11236">
        <property type="entry name" value="PTHR11236"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04715">
        <property type="entry name" value="Anth_synt_I_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00425">
        <property type="entry name" value="Chorismate_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00095">
        <property type="entry name" value="ANTSNTHASEI"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56322">
        <property type="entry name" value="SSF56322"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01820">
        <property type="entry name" value="TrpE-arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="6 8" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="7 8 11" id="KW-0456">Lyase</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0822">Tryptophan biosynthesis</keyword>
    <feature type="domain" description="Anth_synt_I_N" evidence="10">
        <location>
            <begin position="9"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="domain" description="Chorismate_bind" evidence="9">
        <location>
            <begin position="206"/>
            <end position="464"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU364045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF04715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AEF96182.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="477" mass="54624" checksum="9425A3B8D0640208" modified="2011-07-27" version="1">MIKKIKMEVSPLNVYKSIIGENTFLLESAEGVPKIARYSILGKAEGKIEFRNGKLKITPFTEFGEKVKHLEGKYNCPLDALREVRNEYLKHLKHVKEIPSHLRFKGGLVGYLAYDIVRYWIDLDKIQPRPVNDLNFPDAEFFIVKDFISFDLKEKTINLISDSREGIEKLEGILENAKKVDERPNATEENNNENNDNIEIKSNFEKEEFEDAVRKAKEYIYAGDIFQVVLSRRMDVDLRGYDLFEIYKRLREINPSPYMYYLDFGERKLIGSSPEILVKTDIRDGKRYIISRPIAGTIGRGKTEEEDKKLEEILLNDEKERAEHVMLVDLARNDVGKVSKFGTVEVDEFMVIEKYSHVQHIVSNVIGELKDNYDSFLAVKATFPAGTLSGAPKVRAMEIIEELEKTYRGAYGGGIGYFGWDDLMDLAITIRTIEVVNDKGYIQVGAGIVADSVPEKEWEETEKKGMAMVSTIKSIKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCM5</accession>
    <name>F6BCM5_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">CTP synthase</fullName>
            <ecNumber evidence="9">6.3.4.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Cytidine 5'-triphosphate synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Cytidine triphosphate synthetase</fullName>
            <shortName evidence="9">CTP synthetase</shortName>
            <shortName evidence="9">CTPS</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">UTP--ammonia ligase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">pyrG</name>
        <name evidence="12" type="ordered locus">Metig_0686</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen. Regulates intracellular CTP levels through interactions with the four ribonucleotide triphosphates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>ATP + H2O + L-glutamine + UTP = ADP + CTP + 2 H(+) + L-glutamate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:26426"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.4.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>ATP + NH4(+) + UTP = ADP + CTP + 2 H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:16597"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>H2O + L-glutamine = L-glutamate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:15889"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </reaction>
    </comment>
    <comment type="activity regulation">
        <text evidence="9">Allosterically activated by GTP, when glutamine is the substrate; GTP has no effect on the reaction when ammonia is the substrate. The allosteric effector GTP functions by stabilizing the protein conformation that binds the tetrahedral intermediate(s) formed during glutamine hydrolysis. Inhibited by the product CTP, via allosteric rather than competitive inhibition.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 9">Pyrimidine metabolism; CTP biosynthesis via de novo pathway; CTP from UDP: step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Homotetramer.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="9">CTPSs have evolved a hybrid strategy for distinguishing between UTP and CTP. The overlapping regions of the product feedback inhibitory and substrate sites recognize a common feature in both compounds, the triphosphate moiety. To differentiate isosteric substrate and product pyrimidine rings, an additional pocket far from the expected kinase/ligase catalytic site, specifically recognizes the cytosine and ribose portions of the product inhibitor.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the CTP synthase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.2" evidence="9"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96236.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798839.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0686"/>
    <dbReference type="MEROPS" id="C26.964"/>
    <dbReference type="EnsemblBacteria" id="AEF96236">
        <property type="protein sequence ID" value="AEF96236"/>
        <property type="gene ID" value="Metig_0686"/>
    </dbReference>
    <dbReference type="GeneID" id="10643526"/>
    <dbReference type="KEGG" id="mig:Metig_0686"/>
    <dbReference type="HOGENOM" id="CLU_011675_5_0_2"/>
    <dbReference type="OMA" id="EFNNAYR"/>
    <dbReference type="OrthoDB" id="14061at2157"/>
    <dbReference type="UniPathway" id="UPA00159">
        <property type="reaction ID" value="UER00277"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003883">
        <property type="term" value="F:CTP synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044210">
        <property type="term" value="P:'de novo' CTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006541">
        <property type="term" value="P:glutamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03113">
        <property type="entry name" value="CTPS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01746">
        <property type="entry name" value="GATase1_CTP_Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01227">
        <property type="entry name" value="PyrG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004468">
        <property type="entry name" value="CTP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017456">
        <property type="entry name" value="CTP_synthase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017926">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033828">
        <property type="entry name" value="GATase1_CTP_Synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11550">
        <property type="entry name" value="PTHR11550"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06418">
        <property type="entry name" value="CTP_synth_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00117">
        <property type="entry name" value="GATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="SSF52317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00337">
        <property type="entry name" value="PyrG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 9" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="4 9 12" id="KW-0436">Ligase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8 9" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CTP_synth_N" evidence="11">
        <location>
            <begin position="2"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="domain" description="GATase" evidence="10">
        <location>
            <begin position="299"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="region of interest" description="Amidoligase domain" evidence="9">
        <location>
            <begin position="1"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="region of interest" description="L-glutamine binding" evidence="9">
        <location>
            <begin position="385"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile; for glutamine hydrolysis" evidence="9">
        <location>
            <position position="384"/>
        </location>
    </feature>
    <feature type="active site" evidence="9">
        <location>
            <position position="510"/>
        </location>
    </feature>
    <feature type="active site" evidence="9">
        <location>
            <position position="512"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="12"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="13"/>
            <end position="18"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="53"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="70"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="138"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="145"/>
            <end position="147"/>
        </location>
        <ligand>
            <name>CTP</name>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <note>allosteric inhibitor</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="185"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="221"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="237"/>
            <end position="239"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="357"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="408"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="465"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF06418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AEF96236.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="538" mass="60614" checksum="01FCB7601617532D" modified="2011-07-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCR6</accession>
    <name>F6BCR6_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ATPase_AAA_core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0727</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96277.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798880.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96277">
        <property type="protein sequence ID" value="AEF96277"/>
        <property type="gene ID" value="Metig_0727"/>
    </dbReference>
    <dbReference type="GeneID" id="10643567"/>
    <dbReference type="KEGG" id="mig:Metig_0727"/>
    <dbReference type="HOGENOM" id="CLU_063816_1_1_2"/>
    <dbReference type="OMA" id="VISTHSI"/>
    <dbReference type="OrthoDB" id="38516at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13304">
        <property type="entry name" value="AAA_21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATPase_AAA_core" evidence="1">
        <location>
            <begin position="27"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96277.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="335" mass="38838" checksum="8934D34A6E0AC5C7" modified="2011-07-27" version="1">MLVNEIKNIKEFKGINKLKKPLGLGKFNILIGKNNSGKTTILESLFLFPSPGRVYKLTPHNKTILNFISGGRSGIKSLVYKYEGKGNIELNVNLNKKSHRWKITIFSEGNFEVFKDGKKCVGIYGDYLEFLKDNQKPIHGGNIEKISLDSLETVYFPYDTTFIRTLDNFLSHNEKRIIKENIHIKVANTISEVLDEKFTEIVLKKDGWYLRREDASYIHVDDMGDGVKKVVRTMLILELLKPKLILWDDFDTAMHPSMVKNLLNYLIKKDWQVVISTHSIDVLYYILDMDEDIRVITLRKTQSDILEHNTFSIEDIEDIIDANLDPRYVCQNFEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCR9</accession>
    <name>F6BCR9_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">N(4)-bis(aminopropyl)spermidine synthase</fullName>
            <ecNumber evidence="1">2.5.1.128</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Branched-chain polyamine synthase A</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">bpsA</name>
        <name evidence="3" type="ordered locus">Metig_0730</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in the biosynthesis of branched-chain polyamines, which support the growth of thermophiles under high-temperature conditions. Catalyzes the sequential condensation of spermidine with the aminopropyl groups of decarboxylated S-adenosylmethionines to produce N(4)-bis(aminopropyl)spermidine via N(4)-aminopropylspermidine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2 S-adenosyl 3-(methylsulfanyl)propylamine + spermidine = 2 H(+) + N(4)-bis(aminopropyl)spermidine + 2 S-methyl-5'-thioadenosine</text>
            <dbReference type="Rhea" id="RHEA:44132"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17509"/>
            <dbReference type="ChEBI" id="CHEBI:57443"/>
            <dbReference type="ChEBI" id="CHEBI:57834"/>
            <dbReference type="ChEBI" id="CHEBI:82771"/>
            <dbReference type="EC" id="2.5.1.128"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amine and polyamine biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the branched-chain polyamine synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.128" evidence="1"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96280.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798883.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0730"/>
    <dbReference type="EnsemblBacteria" id="AEF96280">
        <property type="protein sequence ID" value="AEF96280"/>
        <property type="gene ID" value="Metig_0730"/>
    </dbReference>
    <dbReference type="GeneID" id="10643570"/>
    <dbReference type="KEGG" id="mig:Metig_0730"/>
    <dbReference type="HOGENOM" id="CLU_042160_0_0_2"/>
    <dbReference type="OMA" id="KSYMFRI"/>
    <dbReference type="OrthoDB" id="23573at2157"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00131"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016765">
        <property type="term" value="F:transferase activity, transferring alkyl or aryl (other than methyl) groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006596">
        <property type="term" value="P:polyamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01947">
        <property type="entry name" value="Aminopropyltransf_BpsA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014435">
        <property type="entry name" value="BpsA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002723">
        <property type="entry name" value="BpsA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01861">
        <property type="entry name" value="DUF43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005895">
        <property type="entry name" value="UCP005895_mtase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1" id="KW-0620">Polyamine biosynthesis</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DUF43" evidence="2">
        <location>
            <begin position="106"/>
            <end position="350"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF96280.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="351" mass="40185" checksum="76A95C8417AEFF27" modified="2011-07-27" version="1">MKEIVAKVKERTSIPVYERSVENVISAIMTSGDFWSIVDVSEEPLPLVCDIIDVLKENGYVEIKENEIVLTNKGEEFAKELGIGPKINATCKCCEGRAVSLDDYKDLLERFKEIVKNRPQPLHEFDQGYVTPETTVSRVILMHSRGDLLNKEVFVLGDDDLTSIALMLSGLPKRIAVVDIDDRLIKFIEKTADEIGYNNLEILTLDIRKPLPDYLVGKFDTFITDPPETLNAVRTFIGRGIATLKGPRCAGYFGITRRESSIDKWRNLQKILINEFNVVITDIIRNFNHYINWGYEKETKAWRLAPVKVLPKDIWYRSYMYRIETLEGSKGLMEEMNVGQELYDDEESSTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>F6BD00</accession>
    <name>F6BD00_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Putative cobalt transport protein CbiM</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Energy-coupling factor transporter probable substrate-capture protein CbiM</fullName>
            <shortName evidence="11">ECF transporter S component CbiM</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">cbiM</name>
        <name evidence="12" type="ordered locus">Metig_0816</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Part of the energy-coupling factor (ECF) transporter complex CbiMNOQ involved in cobalt import.</text>
    </comment>
    <comment type="pathway">
        <text evidence="11">Cofactor biosynthesis; adenosylcobalamin biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Forms an energy-coupling factor (ECF) transporter complex composed of an ATP-binding protein (A component, CbiO), a transmembrane protein (T component, CbiQ) and 2 possible substrate-capture proteins (S components, CbiM and CbiN) of unknown stoichimetry.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Cell membrane</location>
            <topology evidence="11">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the CbiM family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96361.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0816"/>
    <dbReference type="EnsemblBacteria" id="AEF96361">
        <property type="protein sequence ID" value="AEF96361"/>
        <property type="gene ID" value="Metig_0816"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0816"/>
    <dbReference type="HOGENOM" id="CLU_052508_3_0_2"/>
    <dbReference type="OMA" id="ANVFSMG"/>
    <dbReference type="UniPathway" id="UPA00148"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015087">
        <property type="term" value="F:cobalt ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01462">
        <property type="entry name" value="CbiM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018024">
        <property type="entry name" value="CbiM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002751">
        <property type="entry name" value="CbiM/NikMN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43627">
        <property type="entry name" value="PTHR43627"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01891">
        <property type="entry name" value="CbiM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00123">
        <property type="entry name" value="cbiM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 11" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 11" id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword evidence="10 11" id="KW-0170">Cobalt</keyword>
    <keyword evidence="2 11" id="KW-0171">Cobalt transport</keyword>
    <keyword evidence="8 11" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="38"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="75"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="105"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="136"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="172"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022426"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AEF96361.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="228" mass="24381" checksum="590F67CC1350473D" modified="2011-07-27" version="1">MEGFLPPMWCVFWYVLSGIVVVYGIVQLNKIMKENPEYKPLIALAGAFMFVLSSLKMPSVTGSCSHPCGNGLGAVLFGPAVTAVLGAIVLLFQALLLAHGGITTLGANDFSMGIMGPFAGWLVWKALKGKINSTWAIMLAAIFADWVTYVTTSIQLALAYPIPDFSSAFAKFATIFAATQIPLAIAEGLLTAFLWDYIKMLRPDILLKLGVIDESEVPNVSVSPSGGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>F6BD04</accession>
    <name>F6BD04_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">ABC transporter ATP-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Metig_0820</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Part of an ABC transporter complex. Responsible for energy coupling to the transport system.</text>
    </comment>
    <comment type="function">
        <text evidence="3">Probably part of an ABC transporter complex. Responsible for energy coupling to the transport system.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the ABC transporter superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96365.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798967.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0820"/>
    <dbReference type="EnsemblBacteria" id="AEF96365">
        <property type="protein sequence ID" value="AEF96365"/>
        <property type="gene ID" value="Metig_0820"/>
    </dbReference>
    <dbReference type="GeneID" id="10643661"/>
    <dbReference type="KEGG" id="mig:Metig_0820"/>
    <dbReference type="HOGENOM" id="CLU_000604_1_22_2"/>
    <dbReference type="OMA" id="YALELCP"/>
    <dbReference type="OrthoDB" id="92866at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006824">
        <property type="term" value="P:cobalt ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03225">
        <property type="entry name" value="ABC_cobalt_CbiO_domain1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015856">
        <property type="entry name" value="ABC_transpr_CbiO/EcfA_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005876">
        <property type="entry name" value="Co_trans_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01166">
        <property type="entry name" value="cbiO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="1 4 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="6">
        <location>
            <begin position="4"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="37"/>
            <end position="44"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00025157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU364103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEF96365.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="280" mass="31069" checksum="BB9765CAAEF2D029" modified="2011-07-27" version="1">MNIIETRDLHFQYPDGTKALNGINFMAKKGEMIALLGPNGAGKSTLFLHFNGILKPTKGEVLIKGKPIKYNSKDLIEVRKTVGIVFQNPDDQLFAPTVKQDVAFGPLNLGLSEDEVERRVKEALKAVGMEGFGDKPPHHLSGGQKKRVAIAGILAMEPEVIVLDEPTAGLDPLGASQIMKLLYDLNRKGMTIIISTHDVDLVPIYADKVYVMSKGQIIKGGTPKEVFEDVETIRKANLRLPRVAHLVEILRDKDKLPIEMGFTIGEVRRNLKKYLQETHE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F6BD05</accession>
    <name>F6BD05_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">tRNA(Phe) (4-demethylwyosine(37)-C(7)) aminocarboxypropyltransferase</fullName>
            <ecNumber evidence="3">2.5.1.114</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">tRNA wyosine derivatives biosynthesis protein Taw2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">taw2</name>
        <name evidence="5" type="ordered locus">Metig_0821</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">S-adenosyl-L-methionine-dependent transferase that acts as a component of the wyosine derivatives biosynthesis pathway. Catalyzes the transfer of the alpha-amino-alpha-carboxypropyl (acp) group from S-adenosyl-L-methionine to 4-demethylwyosine (imG-14), forming 7-aminocarboxypropyl-demethylwyosine (wybutosine-86) at position 37 of tRNA(Phe).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>4-demethylwyosine(37) in tRNA(Phe) + S-adenosyl-L-methionine = 4-demethyl-7-[(3S)-3-amino-3-carboxypropyl]wyosine(37) in tRNA(Phe) + H(+) + S-methyl-5'-thioadenosine</text>
            <dbReference type="Rhea" id="RHEA:36355"/>
            <dbReference type="Rhea" id="RHEA-COMP:10164"/>
            <dbReference type="Rhea" id="RHEA-COMP:10378"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17509"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:64315"/>
            <dbReference type="ChEBI" id="CHEBI:73550"/>
            <dbReference type="EC" id="2.5.1.114"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the class I-like SAM-binding methyltransferase superfamily. TRM5/TYW2 family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.114" evidence="3"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96366.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0821"/>
    <dbReference type="EnsemblBacteria" id="AEF96366">
        <property type="protein sequence ID" value="AEF96366"/>
        <property type="gene ID" value="Metig_0821"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0821"/>
    <dbReference type="HOGENOM" id="CLU_022610_0_0_2"/>
    <dbReference type="OMA" id="IHYHETV"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102522">
        <property type="term" value="F:tRNA 4-demethylwyosine alpha-amino-alpha-carboxypropyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006400">
        <property type="term" value="P:tRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01922">
        <property type="entry name" value="TYW2_archaea"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030382">
        <property type="entry name" value="MeTrfase_TRM5/TYW2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030867">
        <property type="entry name" value="TYW2_archaea"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02475">
        <property type="entry name" value="Met_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51684">
        <property type="entry name" value="SAM_MT_TRM5_TYW2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="1 3" id="KW-0808">Transferase</keyword>
    <keyword evidence="3" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="SAM_MT_TRM5_TYW2" evidence="4">
        <location>
            <begin position="5"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="region of interest" description="S-adenosyl-L-methionine binding" evidence="3">
        <location>
            <begin position="154"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="80"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="87"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="127"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEF96366.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="252" mass="29830" checksum="CE856352F30B355B" modified="2011-07-27" version="1">MRIKYQKIGDILIVKDDLNEEEIKYLVEKTKCKTIAKYTAQITGDFRTPHLKILYGKETETIHKEHGCLFKIDVKKIMWSMGNIKERERMAKIGNKDEVVVDMFAGIGYFTIPMAKYSKPKKIYAIEINPDAYHYLCENIKLNKLDNVIPILSDNRKVELKDIADRIIMGYVHKTHKFLDKAFEFLKDMGIIHYHETVAEKIMKIRPIERLKFYAEKNNYKLVDYEIHKIKKYAPGVWHIVVDAEFERIQKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>F6BD36</accession>
    <name>F6BD36_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Fe-S cluster domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Metig_0853</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96397.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798999.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0853"/>
    <dbReference type="EnsemblBacteria" id="AEF96397">
        <property type="protein sequence ID" value="AEF96397"/>
        <property type="gene ID" value="Metig_0853"/>
    </dbReference>
    <dbReference type="GeneID" id="10643694"/>
    <dbReference type="KEGG" id="mig:Metig_0853"/>
    <dbReference type="HOGENOM" id="CLU_087825_0_0_2"/>
    <dbReference type="OMA" id="CMMQKVH"/>
    <dbReference type="OrthoDB" id="63018at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007202">
        <property type="entry name" value="4Fe-4S_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04060">
        <property type="entry name" value="FeS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51656">
        <property type="entry name" value="4FE4S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S" evidence="6">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="46"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEF96397.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="217" mass="25033" checksum="E3B96137FF93DCB7" modified="2011-07-27" version="1">MEERINAILNLLPKYNCKACGYKRCDLFAEALFKGEDVEKCPFLFREDFKENLDKIKELLRDVKTKLEKEEKEIIGLIDGYKADFILAPLPNEHSCRETLLILDKKEIEVGDIIRYRPLGCPITHFAKVIDKNYGLYVVHLIGPCHRIDDKEFNYKDVGIAMVVAFEGIVKEEKIPEVGKTVKFLPEHCMMQKVHSGVVVEVEGKRVYIEGIDLKVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-06-02" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>F6BD69</accession>
    <name>F6BD69_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0887</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96430.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799032.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96430">
        <property type="protein sequence ID" value="AEF96430"/>
        <property type="gene ID" value="Metig_0887"/>
    </dbReference>
    <dbReference type="GeneID" id="10643731"/>
    <dbReference type="KEGG" id="mig:Metig_0887"/>
    <dbReference type="HOGENOM" id="CLU_968416_0_0_2"/>
    <dbReference type="OMA" id="SAQLEWI"/>
    <dbReference type="OrthoDB" id="95135at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96430.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="291" mass="34077" checksum="7269FF483BA34D6F" modified="2011-07-27" version="1">MGLLDKILAVLVAILVVEMIIYTGIQTELYTNTKLRNLSLLDDLNHINTSNSIFINELNKTFNKTYPKMDEFPKNYYEVADKMYPDVKVRNIRTLYAILKNIKLPTYKEEYYDCSEASAQLEWILEGYGFKAYIAESPNPPPGEASLSNAGHMWVIVELDNGDLVAIESTYLTKNHYHPPGIIMGNNRKYLEYSYLYQMYLDYLNKYSNGNYILPKNLDDFIRHYLNPPIDDNYLNALSSYYYNPSKLCDTPEEYVLGEKHGNVIYYVPISQFDWWNHPENKHIKELFENN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2020-08-12" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDD4</accession>
    <name>F6BDD4_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_0952</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96495.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96495">
        <property type="protein sequence ID" value="AEF96495"/>
        <property type="gene ID" value="Metig_0952"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0952"/>
    <dbReference type="HOGENOM" id="CLU_3113141_0_0_2"/>
    <dbReference type="OMA" id="MKEPREG"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF96495.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="54" mass="6203" checksum="E95046ABEA8F184D" modified="2011-07-27" version="1">MKEPREGDELVYDGEFKIYLDKIAKEFLTEVNLILKRSFLRGEYLAVKGYGGGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDH4</accession>
    <name>F6BDH4_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcriptional regulator, LysR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_0993</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LysR transcriptional regulatory family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96535.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799137.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0993"/>
    <dbReference type="EnsemblBacteria" id="AEF96535">
        <property type="protein sequence ID" value="AEF96535"/>
        <property type="gene ID" value="Metig_0993"/>
    </dbReference>
    <dbReference type="GeneID" id="10643840"/>
    <dbReference type="KEGG" id="mig:Metig_0993"/>
    <dbReference type="HOGENOM" id="CLU_039613_6_1_2"/>
    <dbReference type="OMA" id="IEGPCHH"/>
    <dbReference type="OrthoDB" id="47614at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005119">
        <property type="entry name" value="LysR_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000847">
        <property type="entry name" value="Tscrpt_reg_HTH_LysR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00126">
        <property type="entry name" value="HTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03466">
        <property type="entry name" value="LysR_substrate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00039">
        <property type="entry name" value="HTHLYSR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50931">
        <property type="entry name" value="HTH_LYSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH lysR-type" evidence="2">
        <location>
            <begin position="3"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF96535.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="295" mass="32823" checksum="C09DFB37339546D4" modified="2011-07-27" version="1">MDPKISYFQTFLVAAKTKSFSKAAKKLGVTQGTVSNHIAALEKFFDAQLFLRTPEGVDLTPEGKILYEHSEKILDYINNAKQQMRILHEHPEGTIRVAASTTPGEHILPSIIREYRKEFKDVDFEITITDSEKCFKMLEEGSADIAAVGHLYDKNYDYVVIGKDRLVLIVPPNHPLAKKGVATLSDILKEDYIDREKGSGTREVIINALQDRGYSIMDLHVVMSLGSTSSVITAVSEGYGISIISEIAAKKAAEAGLVKIVPVEDLDLTRYLYLVKGKRPKNPSAVKSFWDFVSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-06-02" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDI0</accession>
    <name>F6BDI0_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_0999</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96541.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799143.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96541">
        <property type="protein sequence ID" value="AEF96541"/>
        <property type="gene ID" value="Metig_0999"/>
    </dbReference>
    <dbReference type="GeneID" id="10643846"/>
    <dbReference type="KEGG" id="mig:Metig_0999"/>
    <dbReference type="HOGENOM" id="CLU_024489_0_0_2"/>
    <dbReference type="OMA" id="FAYINKV"/>
    <dbReference type="OrthoDB" id="10251at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF96541.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="584" mass="68032" checksum="0C7B1B5C762B0C10" modified="2011-07-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDK7</accession>
    <name>F6BDK7_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">30S ribosomal protein S27ae</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">rps27ae</name>
        <name evidence="8" type="ordered locus">Metig_1026</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="6">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Part of the 30S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the eukaryotic ribosomal protein eS31 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96568.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799170.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1026"/>
    <dbReference type="EnsemblBacteria" id="AEF96568">
        <property type="protein sequence ID" value="AEF96568"/>
        <property type="gene ID" value="Metig_1026"/>
    </dbReference>
    <dbReference type="GeneID" id="10643880"/>
    <dbReference type="KEGG" id="mig:Metig_1026"/>
    <dbReference type="HOGENOM" id="CLU_179743_2_0_2"/>
    <dbReference type="OMA" id="GVFMAEH"/>
    <dbReference type="OrthoDB" id="123606at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00777">
        <property type="entry name" value="Ribosomal_S27ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002906">
        <property type="entry name" value="Ribosomal_S27a"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022845">
        <property type="entry name" value="Ribosomal_S27ae"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011332">
        <property type="entry name" value="Ribosomal_zn-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01599">
        <property type="entry name" value="Ribosomal_S27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01402">
        <property type="entry name" value="Ribosomal_S27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57829">
        <property type="entry name" value="SSF57829"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 6 8" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3 6" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Ribosomal_S27" evidence="7">
        <location>
            <begin position="6"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="24"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="27"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="42"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="45"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM01402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEF96568.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="58" mass="6784" checksum="2395F23206500545" modified="2011-07-27" version="1">MGKTQKYKYYKIEGDKVVRLKKFCPKCGPGVFMAEHLNRYACGRCGYTEWKNSGKKEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-06-02" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDN8</accession>
    <name>F6BDN8_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Nucleic acid binding OB-fold tRNA/helicase-type</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_1060</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96599.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799200.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96599">
        <property type="protein sequence ID" value="AEF96599"/>
        <property type="gene ID" value="Metig_1060"/>
    </dbReference>
    <dbReference type="GeneID" id="10643914"/>
    <dbReference type="KEGG" id="mig:Metig_1060"/>
    <dbReference type="HOGENOM" id="CLU_2230397_0_0_2"/>
    <dbReference type="OMA" id="VIGMENE"/>
    <dbReference type="OrthoDB" id="108635at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0347">Helicase</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF96599.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="105" mass="11809" checksum="C7DED284C32FFD87" modified="2011-07-27" version="1">MYKLKKISEIKDIDEKVAVIGRVKDIGEKIKVEDDSGEILVSLRKNSNDVQLNINSKVLVFGTTKLTPKGIIILGNVVVVDDADEELLMEFEDIIQKLNKKNKIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2020-06-17" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDP1</accession>
    <name>F6BDP1_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1063</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96602.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96602">
        <property type="protein sequence ID" value="AEF96602"/>
        <property type="gene ID" value="Metig_1063"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_1063"/>
    <dbReference type="HOGENOM" id="CLU_2550277_0_0_2"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="56"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96602.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="82" mass="9460" checksum="FCCFD88AC7EADCB2" modified="2011-07-27" version="1">MKKKMLVAVICITYGLLTKLMGNFDISIIIISTDFLAYGIYCYIRQQLFRKLYSKLHNIVNLLTIVFSFLVSLLILLIALIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDR5</accession>
    <name>F6BDR5_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">3-dehydroquinate synthase</fullName>
            <shortName evidence="3">DHQ synthase</shortName>
            <ecNumber evidence="3">1.4.1.24</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">3-dehydroquinate synthase II</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">aroB'</name>
        <name evidence="4" type="ordered locus">Metig_1087</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the oxidative deamination and cyclization of 2-amino-3,7-dideoxy-D-threo-hept-6-ulosonic acid (ADH) to yield 3-dehydroquinate (DHQ), which is fed into the canonical shikimic pathway of aromatic amino acid biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>2-amino-2,3,7-trideoxy-D-lyxo-hept-6-ulosonate + H2O + NAD(+) = 3-dehydroquinate + H(+) + NADH + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:25956"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:32364"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:58859"/>
            <dbReference type="EC" id="1.4.1.24"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the archaeal-type DHQ synthase family.</text>
    </comment>
    <dbReference type="EC" id="1.4.1.24" evidence="3"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96626.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799227.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1087"/>
    <dbReference type="EnsemblBacteria" id="AEF96626">
        <property type="protein sequence ID" value="AEF96626"/>
        <property type="gene ID" value="Metig_1087"/>
    </dbReference>
    <dbReference type="GeneID" id="10643941"/>
    <dbReference type="KEGG" id="mig:Metig_1087"/>
    <dbReference type="HOGENOM" id="CLU_056379_0_0_2"/>
    <dbReference type="OMA" id="HFGMAIK"/>
    <dbReference type="OrthoDB" id="29853at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003856">
        <property type="term" value="F:3-dehydroquinate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102042">
        <property type="term" value="F:dehydroquinate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009073">
        <property type="term" value="P:aromatic amino acid family biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:cellular amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01244">
        <property type="entry name" value="Arch_DHQ_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002812">
        <property type="entry name" value="DHQ_synth"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33563">
        <property type="entry name" value="PTHR33563"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01959">
        <property type="entry name" value="DHQS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006655">
        <property type="entry name" value="DHQ_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="2 3" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="3" id="KW-0520">NAD</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEF96626.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="361" mass="40386" checksum="67F73DD26A4FBD08" modified="2011-07-27" version="1">MKFAWVNVIGDDWEDRKEIVKCALESSIPTIMAKSEDIEKIKELGNIKVASNDLNADIVVINKNDDLEALREAKKMGKETAIYIPIESKDDEIFASEVSRLDFVDNIILEGRDWTIIPLENLIADLFNEDVKIIAAVNSIKEAKTAYEILEKGVDGVLLFPNDLNDIKELVELLENINAQRVELDVATIKRVEPVGSGDRVCIDTCSLMEIGEGMLIGSYSRGLFLVHSETVENPYVSTRPFRVNAGPVHAYILCPGNKTKYLSELKAGDKVLIVNKDGRAREAIVGRVKIEKRPLMLIEAEYKGDIIRTILQNAETIRLVREDGTPVSIVDLKEGDKVLIKPDENARHFGMAIKETIIEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDT3</accession>
    <name>F6BDT3_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Glutamate-1-semialdehyde 2,1-aminomutase</fullName>
            <shortName evidence="7">GSA</shortName>
            <ecNumber evidence="7">5.4.3.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Glutamate-1-semialdehyde aminotransferase</fullName>
            <shortName evidence="7">GSA-AT</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">hemL</name>
        <name evidence="9" type="ordered locus">Metig_1105</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>(S)-4-amino-5-oxopentanoate = 5-aminolevulinate</text>
            <dbReference type="Rhea" id="RHEA:14265"/>
            <dbReference type="ChEBI" id="CHEBI:57501"/>
            <dbReference type="ChEBI" id="CHEBI:356416"/>
            <dbReference type="EC" id="5.4.3.8"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 7">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2 7">Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; 5-aminolevulinate from L-glutamyl-tRNA(Glu): step 2/2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 7">Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. HemL subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.4.3.8" evidence="7"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96644.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1105"/>
    <dbReference type="EnsemblBacteria" id="AEF96644">
        <property type="protein sequence ID" value="AEF96644"/>
        <property type="gene ID" value="Metig_1105"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_1105"/>
    <dbReference type="HOGENOM" id="CLU_016922_1_5_2"/>
    <dbReference type="OMA" id="WGPLIFG"/>
    <dbReference type="UniPathway" id="UPA00251">
        <property type="reaction ID" value="UER00317"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042286">
        <property type="term" value="F:glutamate-1-semialdehyde 2,1-aminomutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006782">
        <property type="term" value="P:protoporphyrinogen IX biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00610">
        <property type="entry name" value="OAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00375">
        <property type="entry name" value="HemL_aminotrans_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004639">
        <property type="entry name" value="4pyrrol_synth_GluAld_NH2Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005814">
        <property type="entry name" value="Aminotrans_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00202">
        <property type="entry name" value="Aminotran_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00713">
        <property type="entry name" value="hemL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00600">
        <property type="entry name" value="AA_TRANSFER_CLASS_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 7 9" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6 7" id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword evidence="4 7 8" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="7">
        <location>
            <position position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AEF96644.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="424" mass="47321" checksum="7C16F7F5659A8EBE" modified="2011-07-27" version="1">MKLEKSKALFEEAQKYLVKGVNSPVRYFKPYPFFVQKAKDCYLFDVDGNKYIDYCLAYGPMVLGHANEAIINAVKEQLELGTAYGCPTEKEIILAKEVINRVPCAEMVRFVNSGTEATMSAIRLARGVTGRKKIIKFDGAYHGAHDYVLVKSGSGALTHGTPNSPGIPEETTKNTILVPFNDEEAIEKVIKDNKDEIACLIVEPVMGNVGCILPKEGYLKFLREITEENGIILIFDEVITGFRLAKGGAQEYYGVIPDLATLGKILGGGFPIGAIVGKKEIMEQMSPSGPIYQAGTFNGNPVSITAGIETLKQLDDKFYKETTKTAEKLANCLREVSERYNIPAKVYNIASMFQIYFNDKEVLNYEIAKQSDTERFMKYFWNLLENGVFVPPSQFECCFTSIKHNDEVVEKTMNAIEEAFKNLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDT4</accession>
    <name>F6BDT4_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_1106</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96645.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799246.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96645">
        <property type="protein sequence ID" value="AEF96645"/>
        <property type="gene ID" value="Metig_1106"/>
    </dbReference>
    <dbReference type="GeneID" id="10643964"/>
    <dbReference type="KEGG" id="mig:Metig_1106"/>
    <dbReference type="HOGENOM" id="CLU_2447688_0_0_2"/>
    <dbReference type="OrthoDB" id="61088at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF96645.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="89" mass="10368" checksum="79EFAF36B003151C" modified="2011-07-27" version="1">MDESERRKIIEEFINILEMLRPYAAEGSDEKTNIILTLKFLESGKVLDEDLLPIANKILEIAKRIGSYEMKREIELFLVGEKLKRLTKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDV1</accession>
    <name>F6BDV1_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1123</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96662.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96662">
        <property type="protein sequence ID" value="AEF96662"/>
        <property type="gene ID" value="Metig_1123"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_1123"/>
    <dbReference type="HOGENOM" id="CLU_1405986_0_0_2"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="47"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="92"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="120"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="150"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="173"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96662.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="193" mass="22846" checksum="CFB5159BB95953F6" modified="2011-07-27" version="1">MVSDRFFKIMWYLLAFCIIYLVIYGIIGIISNNIIDDDSIYNFVGIFQVFLIIFGYLSVGLCIGGLILVVIRTIWNGVLREYLKDKTSIIQLLLLDYLTTTWFIYIFILCLIVYSKLRNLGISMLVFSVGIWLYLYCWYVFMNYLDKKELRLFVPQIVGLLSTSIIIYKSENYVISVLILLLTSLFWYFWRKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDW8</accession>
    <name>F6BDW8_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">GTP cyclohydrolase MptA</fullName>
            <ecNumber evidence="3">3.5.4.39</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">GTP cyclohydrolase IV</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">mptA</name>
        <name evidence="4" type="ordered locus">Metig_1140</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Converts GTP to 7,8-dihydro-D-neopterin 2',3'-cyclic phosphate, the first intermediate in the biosynthesis of coenzyme methanopterin.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>GTP + H2O = 7,8-dihydroneopterin 2',3'-cyclic phosphate + diphosphate + formate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:25860"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:58854"/>
            <dbReference type="EC" id="3.5.4.39"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
        <text evidence="3">Binds 1 Fe(2+) ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Cofactor biosynthesis; 5,6,7,8-tetrahydromethanopterin biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the GTP cyclohydrolase IV family.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.39" evidence="3"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96679.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799280.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1140"/>
    <dbReference type="EnsemblBacteria" id="AEF96679">
        <property type="protein sequence ID" value="AEF96679"/>
        <property type="gene ID" value="Metig_1140"/>
    </dbReference>
    <dbReference type="GeneID" id="10643998"/>
    <dbReference type="KEGG" id="mig:Metig_1140"/>
    <dbReference type="HOGENOM" id="CLU_062816_1_0_2"/>
    <dbReference type="OMA" id="PCSQGMS"/>
    <dbReference type="OrthoDB" id="109805at2157"/>
    <dbReference type="UniPathway" id="UPA00065"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003933">
        <property type="term" value="F:GTP cyclohydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01527_A">
        <property type="entry name" value="GTP_cyclohydrol_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003801">
        <property type="entry name" value="GTP_cyclohydrolase_FolE2/MptA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022840">
        <property type="entry name" value="GTP_cyclohydrolase_MptA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36445">
        <property type="entry name" value="PTHR36445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02649">
        <property type="entry name" value="GCHY-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00294">
        <property type="entry name" value="TIGR00294"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 3" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="site" description="May be catalytically important" evidence="3">
        <location>
            <position position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEF96679.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="310" mass="35499" checksum="03C5F8800FD810BD" modified="2011-07-27" version="1">MKCDIQATEPDIKVPLTRVGITNLKKLVKIKRKNKRPIILLPTFEVFVDLPSFQKGVHMSRNPEVIEEIIEDAVEKDVYEMETICEEIVKKLFEKHEYATRAEVFMVSDFVLEEKSPVSKKHSQEVCKIMAKACGFKDGNKIKIKKLVGAEVVGITACPCAQNLLKEKSIENLKKQGFSDEEIKKIMDSVVIATHNQRGIGTILIEVPEGYNVEILRIIDIIKKSMSGEVYELLKRSDEAYVVETAHRNPKFVEDCAREMIKRIVETFDYLPDEARVLVRQVNQESIHRHDAFAERCATLGELRKELDVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDX2</accession>
    <name>F6BDX2_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="4">MCM family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metig_1144</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the MCM family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96683.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799284.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1144"/>
    <dbReference type="EnsemblBacteria" id="AEF96683">
        <property type="protein sequence ID" value="AEF96683"/>
        <property type="gene ID" value="Metig_1144"/>
    </dbReference>
    <dbReference type="GeneID" id="10644002"/>
    <dbReference type="KEGG" id="mig:Metig_1144"/>
    <dbReference type="HOGENOM" id="CLU_000995_7_2_2"/>
    <dbReference type="OMA" id="NAYTCDR"/>
    <dbReference type="OrthoDB" id="3067at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032508">
        <property type="term" value="P:DNA duplex unwinding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031327">
        <property type="entry name" value="MCM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018525">
        <property type="entry name" value="MCM_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001208">
        <property type="entry name" value="MCM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041562">
        <property type="entry name" value="MCM_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033762">
        <property type="entry name" value="MCM_OB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11630">
        <property type="entry name" value="PTHR11630"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00493">
        <property type="entry name" value="MCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17855">
        <property type="entry name" value="MCM_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17207">
        <property type="entry name" value="MCM_OB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01657">
        <property type="entry name" value="MCMFAMILY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00350">
        <property type="entry name" value="MCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00847">
        <property type="entry name" value="MCM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50051">
        <property type="entry name" value="MCM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MCM" evidence="3">
        <location>
            <begin position="276"/>
            <end position="478"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU004070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEF96683.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="677" mass="77188" checksum="BCB8E1B83BD11DAE" modified="2011-07-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDY2</accession>
    <name>F6BDY2_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Phosphatidate cytidylyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1154</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96693.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799294.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1154"/>
    <dbReference type="EnsemblBacteria" id="AEF96693">
        <property type="protein sequence ID" value="AEF96693"/>
        <property type="gene ID" value="Metig_1154"/>
    </dbReference>
    <dbReference type="GeneID" id="10644012"/>
    <dbReference type="KEGG" id="mig:Metig_1154"/>
    <dbReference type="HOGENOM" id="CLU_031477_4_2_2"/>
    <dbReference type="OrthoDB" id="91246at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="29"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="67"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="91"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="124"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96693.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="180" mass="20217" checksum="BE57A9573716983B" modified="2011-07-27" version="1">MREIYRQLIHMVFGSITAFSILYFGKKVIYPLFILTLAGIFLHFYLRKHYAPIISDLLRLCGRENEYGKGAVLFAVGVLIAVILVDNINAIFYAILVFSISDALATLVGIRGKIKIFGKTLEGFLAFFISACIILHSFGIYGVLVAFVGAFIELISKKIKIDDNLVLPIFLAFILNMINW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDZ0</accession>
    <name>F6BDZ0_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcriptional regulator, HxlR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1162</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96701.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799302.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1162"/>
    <dbReference type="EnsemblBacteria" id="AEF96701">
        <property type="protein sequence ID" value="AEF96701"/>
        <property type="gene ID" value="Metig_1162"/>
    </dbReference>
    <dbReference type="GeneID" id="10644022"/>
    <dbReference type="KEGG" id="mig:Metig_1162"/>
    <dbReference type="HOGENOM" id="CLU_2217109_0_0_2"/>
    <dbReference type="OMA" id="KWEAKWK"/>
    <dbReference type="OrthoDB" id="112375at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002577">
        <property type="entry name" value="HTH_HxlR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01638">
        <property type="entry name" value="HxlR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51118">
        <property type="entry name" value="HTH_HXLR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH hxlR-type" evidence="1">
        <location>
            <begin position="1"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96701.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="106" mass="12271" checksum="E36550FD28412608" modified="2011-07-27" version="1">MEDIFSLLGKKGVIDIIFKIKEGVNTFTTLKKSLDEEGKGVSTRTLAERLDELEHENIIQKESNKYILTKKGEELIEIIEKVKSWEAKWREVKIPRVILGMLGEKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-09-29" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>F6BE11</accession>
    <name>F6BE11_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="3">AAA ATPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_1184</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the archaeal ATPase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96722.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799321.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96722">
        <property type="protein sequence ID" value="AEF96722"/>
        <property type="gene ID" value="Metig_1184"/>
    </dbReference>
    <dbReference type="GeneID" id="10644044"/>
    <dbReference type="KEGG" id="mig:Metig_1184"/>
    <dbReference type="HOGENOM" id="CLU_068608_0_0_2"/>
    <dbReference type="OrthoDB" id="34326at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011579">
        <property type="entry name" value="ATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01637">
        <property type="entry name" value="ATPase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA" evidence="2">
        <location>
            <begin position="24"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF96722.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="342" mass="40775" checksum="B731A43B7BD8BD29" modified="2011-07-27" version="1">MQKFKFYDREKELNYLKTYCQLLPNSILFVYGPKSSGKSTVMKKVIKDLENRGDLVFFYYNLREYATPTKEEFLNIFFEKGDKKYIQNSLVIDIKVFKFGIEENFDFNNVSLNDVFRRIKEDINTVVKEKKIPILIIDELQKLKNIYFNGGKSLLNELFNLFVSLTKMEHLCHVICLTSDSLFINEIYTNSSLAETSEYYLIDWLKKEDIKRILKEEGFNEKEVNFAVNYVSLPYEITQLINNRKLGVSVEDTIRNWINVERDRLKYLIDTSDIEEERILNALKKFKDKIKISYVKDVKKEEFSVFKYLIENEILFYDVVNGIIKPHSLKKIYAIRRILEER</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>F6BE62</accession>
    <name>F6BE62_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Coenzyme F420-reducing hydrogenase delta subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_1201</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96739.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799338.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1201"/>
    <dbReference type="EnsemblBacteria" id="AEF96739">
        <property type="protein sequence ID" value="AEF96739"/>
        <property type="gene ID" value="Metig_1201"/>
    </dbReference>
    <dbReference type="GeneID" id="10644061"/>
    <dbReference type="KEGG" id="mig:Metig_1201"/>
    <dbReference type="HOGENOM" id="CLU_099037_0_2_2"/>
    <dbReference type="OMA" id="NWTLSEP"/>
    <dbReference type="OrthoDB" id="122958at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008047">
        <property type="term" value="F:enzyme activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06064">
        <property type="entry name" value="H2MP_F420-Reduc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004411">
        <property type="entry name" value="Pept_A31_F420-red_hyd_d"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023430">
        <property type="entry name" value="Pept_HybD-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000671">
        <property type="entry name" value="Peptidase_A31"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30302">
        <property type="entry name" value="PTHR30302"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01750">
        <property type="entry name" value="HycI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00446">
        <property type="entry name" value="HYDRGNUPTAKE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53163">
        <property type="entry name" value="SSF53163"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00130">
        <property type="entry name" value="frhD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00072">
        <property type="entry name" value="hydrog_prot"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF96739.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="166" mass="18272" checksum="392E6F57C340F616" modified="2011-07-27" version="1">MPEYLNKEILILGCGNVLFGDDGFGYAVVNKLNALKEKIPALNSNKIGIIDAGTGASHYILSLIDEDTPIKKIIIIDAIDFGLKPGELIKLYPNDLPKIKKYYIDAHDMPLAGLIKEINEKYGIEVVVIGCQSKNMTAPDICLELSEEVKNAVDRAVEMVLDELLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>F6BE68</accession>
    <name>F6BE68_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Nitrogenase iron protein</fullName>
            <ecNumber evidence="12">1.18.6.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Nitrogenase Fe protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Nitrogenase component II</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Nitrogenase reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">nifH</name>
        <name evidence="15" type="ordered locus">Metig_1207</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 12">The key enzymatic reactions in nitrogen fixation are catalyzed by the nitrogenase complex, which has 2 components: the iron protein and the molybdenum-iron protein.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 12">
            <text>16 ATP + 16 H2O + N2 + 8 reduced [2Fe-2S]-[ferredoxin] = 16 ADP + 6 H(+) + H2 + 2 NH4(+) + 8 oxidized [2Fe-2S]-[ferredoxin] + 16 phosphate</text>
            <dbReference type="Rhea" id="RHEA:21448"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17997"/>
            <dbReference type="ChEBI" id="CHEBI:18276"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="1.18.6.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="12">Binds 1 [4Fe-4S] cluster per dimer.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4 12">Homodimer.</text>
    </comment>
    <comment type="PTM">
        <text evidence="13">The reversible ADP-ribosylation of Arg inactivates the nitrogenase reductase and regulates nitrogenase activity.</text>
    </comment>
    <comment type="PTM">
        <text evidence="12">The reversible ADP-ribosylation of Arg-107 inactivates the nitrogenase reductase and regulates nitrogenase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 12 14">Belongs to the NifH/BchL/ChlL family.</text>
    </comment>
    <dbReference type="EC" id="1.18.6.1" evidence="12"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96745.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799344.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1207"/>
    <dbReference type="EnsemblBacteria" id="AEF96745">
        <property type="protein sequence ID" value="AEF96745"/>
        <property type="gene ID" value="Metig_1207"/>
    </dbReference>
    <dbReference type="GeneID" id="10644067"/>
    <dbReference type="KEGG" id="mig:Metig_1207"/>
    <dbReference type="HOGENOM" id="CLU_059373_0_0_2"/>
    <dbReference type="OMA" id="TEIAQCY"/>
    <dbReference type="OrthoDB" id="66372at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018697">
        <property type="term" value="F:carbonyl sulfide nitrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016163">
        <property type="term" value="F:nitrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009399">
        <property type="term" value="P:nitrogen fixation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00533">
        <property type="entry name" value="NifH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030655">
        <property type="entry name" value="NifH/chlL_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000392">
        <property type="entry name" value="NifH/frxC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005977">
        <property type="entry name" value="Nitrogenase_Fe_NifH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42864">
        <property type="entry name" value="PTHR42864"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00142">
        <property type="entry name" value="Fer4_NifH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000363">
        <property type="entry name" value="Nitrogenase_iron"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01287">
        <property type="entry name" value="nifH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00746">
        <property type="entry name" value="NIFH_FRXC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00692">
        <property type="entry name" value="NIFH_FRXC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51026">
        <property type="entry name" value="NIFH_FRXC_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 14" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="7 12 13" id="KW-0013">ADP-ribosylation</keyword>
    <keyword evidence="8 12 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 12 14" id="KW-0408">Iron</keyword>
    <keyword evidence="10 12 14" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="5 12 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 12" id="KW-0535">Nitrogen fixation</keyword>
    <keyword evidence="6 12 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12 14 15" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="8"/>
            <end position="15"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="104"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="140"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="modified residue" description="ADP-ribosylarginine; by dinitrogenase reductase ADP-ribosyltransferase" evidence="12 13">
        <location>
            <position position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR605977-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU003688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="AEF96745.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="288" mass="31463" checksum="BCFD8880136C3A24" modified="2011-07-27" version="1">MKQIAFYGKGGIGKSTTVCNIAAALADEGKRVMVVGCDPKHDCTSNLRGGEEIPTVLDTLREKGMEKMSLSDIEKRIDIDEIVYKGYKGIYCIEAGGPKPGYGCAGRGVIVAIDLLKKMNVFEELGVDVVLYDVLGDVVCGGFAMPLRMGLAEQIYIVTSSDYMAMYAANNICRGMKEFAKRGGSRLGGLIYNVRGSLDAEDIVTEFAKKLGTEIIGKIPNSLLIAEAEIEGKTVIEYAPDSEIASIYRELAKKIYKNKNGVIPNPLENEEIMQIGKRVKERIRSLRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>F6BE72</accession>
    <name>F6BE72_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1211</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96749.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799348.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1211"/>
    <dbReference type="EnsemblBacteria" id="AEF96749">
        <property type="protein sequence ID" value="AEF96749"/>
        <property type="gene ID" value="Metig_1211"/>
    </dbReference>
    <dbReference type="GeneID" id="10644071"/>
    <dbReference type="KEGG" id="mig:Metig_1211"/>
    <dbReference type="HOGENOM" id="CLU_1718221_0_0_2"/>
    <dbReference type="OMA" id="VNWIVNN"/>
    <dbReference type="OrthoDB" id="88957at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="63"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="94"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="127"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96749.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="152" mass="16112" checksum="C57F1D82A0AC6E53" modified="2011-07-27" version="1">MRISPLVAGLIGGFSAALLQAFFKVSPPPAYGICIACHTRDLVNWIVNAISGSTLFVAPVSKVFPVLTVVGIFVGAFIAANVHKEFKIKQTHNPIVGFVLGVLVVIFALLMGGCPLRETIRTAYGDVIALISLIAMFAGVIVASEVYLKRNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEA8</accession>
    <name>F6BEA8_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CRISPR system Cms protein Csm2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">CRISPR type III A-associated protein Csm2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Metig_1248</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">This subunit may be involved in monitoring complementarity of crRNA and target RNA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CRISPR-associated Csm2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96785.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799384.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96785">
        <property type="protein sequence ID" value="AEF96785"/>
        <property type="gene ID" value="Metig_1248"/>
    </dbReference>
    <dbReference type="GeneID" id="10644111"/>
    <dbReference type="KEGG" id="mig:Metig_1248"/>
    <dbReference type="HOGENOM" id="CLU_131491_2_0_2"/>
    <dbReference type="OMA" id="KPKLAYN"/>
    <dbReference type="OrthoDB" id="127804at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010149">
        <property type="entry name" value="CRISPR-assoc_prot_Csm2_III-A"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03750">
        <property type="entry name" value="Csm2_III-A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01870">
        <property type="entry name" value="cas_TM1810_Csm2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0051">Antiviral defense</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0694">RNA-binding</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00031723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEF96785.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="149" mass="18060" checksum="D971B1ADD3860F92" modified="2011-07-27" version="1">MGYSKYQNPNPKNENKAGKNSDKLKLLDEILNVNDKNIKKFLNYAEEFANKNLKYISFTKIRKFYDYLNEISPEDEDWIIKFAHLKPMVAYHYGKEKRNQGLFELKKLIDAVFDKIYNENDENKRKEMFKHFKKFFEAIIAYKRFYEGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEB9</accession>
    <name>F6BEB9_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_1259</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96796.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799394.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1259"/>
    <dbReference type="EnsemblBacteria" id="AEF96796">
        <property type="protein sequence ID" value="AEF96796"/>
        <property type="gene ID" value="Metig_1259"/>
    </dbReference>
    <dbReference type="GeneID" id="10644122"/>
    <dbReference type="KEGG" id="mig:Metig_1259"/>
    <dbReference type="HOGENOM" id="CLU_1736436_0_0_2"/>
    <dbReference type="OMA" id="MKIPKIY"/>
    <dbReference type="OrthoDB" id="84603at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019217">
        <property type="entry name" value="DUF2118"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09891">
        <property type="entry name" value="DUF2118"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF96796.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="153" mass="17748" checksum="58CAA5E2B770ED17" modified="2011-07-27" version="1">MKIPRLYVEGETEKNEGRKVVIENDGKVIRFLDEDEEYEGEGKVLYQVIYDDFDKYILLSKLTRDVLIQYPDKRTITYLKKGTELLEIPAEGYEVHPIVDFGCRVLEGHRLAALVTKKGEIRFVNTPVTGTVVFMKEVPAKRANYVFYILPEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEC4</accession>
    <name>F6BEC4_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Valine--tRNA ligase</fullName>
            <ecNumber evidence="6">6.1.1.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Valyl-tRNA synthetase</fullName>
            <shortName evidence="6">ValRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">valS</name>
        <name evidence="10" type="ordered locus">Metig_1264</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes the attachment of valine to tRNA(Val). As ValRS can inadvertently accommodate and process structurally similar amino acids such as threonine, to avoid such errors, it has a 'posttransfer' editing activity that hydrolyzes mischarged Thr-tRNA(Val) in a tRNA-dependent manner.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>ATP + L-valine + tRNA(Val) = AMP + diphosphate + L-valyl-tRNA(Val)</text>
            <dbReference type="Rhea" id="RHEA:10704"/>
            <dbReference type="Rhea" id="RHEA-COMP:9672"/>
            <dbReference type="Rhea" id="RHEA-COMP:9708"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57762"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78537"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.9"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="6">ValRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated threonine is translocated from the active site to the editing site.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the class-I aminoacyl-tRNA synthetase family. ValS type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.9" evidence="6"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96801.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799399.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1264"/>
    <dbReference type="EnsemblBacteria" id="AEF96801">
        <property type="protein sequence ID" value="AEF96801"/>
        <property type="gene ID" value="Metig_1264"/>
    </dbReference>
    <dbReference type="GeneID" id="10644127"/>
    <dbReference type="KEGG" id="mig:Metig_1264"/>
    <dbReference type="HOGENOM" id="CLU_001493_0_2_2"/>
    <dbReference type="OMA" id="FATKLWN"/>
    <dbReference type="OrthoDB" id="4914at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002161">
        <property type="term" value="F:aminoacyl-tRNA editing activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004832">
        <property type="term" value="F:valine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006438">
        <property type="term" value="P:valyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07962">
        <property type="entry name" value="Anticodon_Ia_Val"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02005">
        <property type="entry name" value="Val_tRNA_synth_type2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002300">
        <property type="entry name" value="aa-tRNA-synth_Ia"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033705">
        <property type="entry name" value="Anticodon_Ia_Val"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013155">
        <property type="entry name" value="M/V/L/I-tRNA-synth_anticd-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009008">
        <property type="entry name" value="Val/Leu/Ile-tRNA-synth_edit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022874">
        <property type="entry name" value="Valine-tRNA_ligase_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002303">
        <property type="entry name" value="Valyl-tRNA_ligase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11946">
        <property type="entry name" value="PTHR11946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08264">
        <property type="entry name" value="Anticodon_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00133">
        <property type="entry name" value="tRNA-synt_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00986">
        <property type="entry name" value="TRNASYNTHVAL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="SSF47323"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50677">
        <property type="entry name" value="SSF50677"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00422">
        <property type="entry name" value="valS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6 7" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="3 6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1 6 7" id="KW-0436">Ligase</keyword>
    <keyword evidence="2 6 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4 6 7" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="tRNA-synt_1" evidence="8">
        <location>
            <begin position="16"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="domain" description="Anticodon_1" evidence="9">
        <location>
            <begin position="614"/>
            <end position="762"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'HIGH' region" evidence="6">
        <location>
            <begin position="45"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'KMSKS' region" evidence="6">
        <location>
            <begin position="530"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="533"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF08264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AEF96801.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="887" mass="103829" checksum="CA27D9E739A6CAB0" modified="2011-07-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEF8</accession>
    <name>F6BEF8_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">PilT protein domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0058</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95619.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798228.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0058"/>
    <dbReference type="EnsemblBacteria" id="AEF95619">
        <property type="protein sequence ID" value="AEF95619"/>
        <property type="gene ID" value="Metig_0058"/>
    </dbReference>
    <dbReference type="GeneID" id="10642893"/>
    <dbReference type="KEGG" id="mig:Metig_0058"/>
    <dbReference type="HOGENOM" id="CLU_107892_1_0_2"/>
    <dbReference type="OMA" id="DEMIINY"/>
    <dbReference type="OrthoDB" id="118267at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041120">
        <property type="entry name" value="PIN_9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002716">
        <property type="entry name" value="PIN_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18477">
        <property type="entry name" value="PIN_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00670">
        <property type="entry name" value="PINc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="SSF88723"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PINc" evidence="1">
        <location>
            <begin position="2"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF95619.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="130" mass="15371" checksum="77DD9287DB4ABACA" modified="2011-07-27" version="1">MYLVVPDTNFLIYAFKHKINFDYELERALNAKYRVVILKCIYDELQKLQRELKGKEKLSVSLALKMIEKYGIIDYNKGTYTDEIIINFAKENKNVIVCTNDKELKNKLIDLNIPIIFVRQKNYFDVIGLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEG5</accession>
    <name>F6BEG5_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Carbamoyl-phosphate synthase (glutamine-hydrolyzing)</fullName>
            <ecNumber evidence="7">6.3.5.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Carbamoyl-phosphate synthetase ammonia chain</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="ordered locus">Metig_0065</name>
    </gene>
    <organism evidence="20">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4">Amino-acid biosynthesis; L-arginine biosynthesis; carbamoyl phosphate from bicarbonate: step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 1/3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the CarB family.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.5" evidence="7"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95626.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798235.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0065"/>
    <dbReference type="EnsemblBacteria" id="AEF95626">
        <property type="protein sequence ID" value="AEF95626"/>
        <property type="gene ID" value="Metig_0065"/>
    </dbReference>
    <dbReference type="GeneID" id="10642900"/>
    <dbReference type="KEGG" id="mig:Metig_0065"/>
    <dbReference type="HOGENOM" id="CLU_000513_3_4_2"/>
    <dbReference type="OMA" id="CVQAVFA"/>
    <dbReference type="OrthoDB" id="4638at2157"/>
    <dbReference type="UniPathway" id="UPA00068">
        <property type="reaction ID" value="UER00171"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00070">
        <property type="reaction ID" value="UER00115"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004088">
        <property type="term" value="F:carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044205">
        <property type="term" value="P:'de novo' UMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006526">
        <property type="term" value="P:arginine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd01424">
        <property type="entry name" value="MGS_CPS_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1030.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1380">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005480">
        <property type="entry name" value="CarbamoylP_synth_lsu_oligo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036897">
        <property type="entry name" value="CarbamoylP_synth_lsu_oligo_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005479">
        <property type="entry name" value="CbamoylP_synth_lsu-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005483">
        <property type="entry name" value="CbamoylP_synth_lsu_CPSase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011607">
        <property type="entry name" value="MGS-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036914">
        <property type="entry name" value="MGS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033937">
        <property type="entry name" value="MGS_CPS_CarB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02786">
        <property type="entry name" value="CPSase_L_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02142">
        <property type="entry name" value="MGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00098">
        <property type="entry name" value="CPSASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01096">
        <property type="entry name" value="CPSase_L_D3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00851">
        <property type="entry name" value="MGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48108">
        <property type="entry name" value="SSF48108"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52335">
        <property type="entry name" value="SSF52335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="SSF52440"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00866">
        <property type="entry name" value="CPSASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00867">
        <property type="entry name" value="CPSASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51855">
        <property type="entry name" value="MGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0055">Arginine biosynthesis</keyword>
    <keyword evidence="16" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 19" id="KW-0436">Ligase</keyword>
    <keyword evidence="12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="14" id="KW-0464">Manganese</keyword>
    <keyword evidence="11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-grasp" evidence="17">
        <location>
            <begin position="207"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="domain" description="MGS-like" evidence="18">
        <location>
            <begin position="465"/>
            <end position="602"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00009799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00011275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00012738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00030577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="AEF95626.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="602" mass="66539" checksum="84CC63F28E1B0E53" modified="2011-07-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEG6</accession>
    <name>F6BEG6_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Fructose-bisphosphatase</fullName>
            <ecNumber evidence="1">3.1.3.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metig_0066</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.1.3.11" evidence="1"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95627.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798236.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0066"/>
    <dbReference type="EnsemblBacteria" id="AEF95627">
        <property type="protein sequence ID" value="AEF95627"/>
        <property type="gene ID" value="Metig_0066"/>
    </dbReference>
    <dbReference type="GeneID" id="10642901"/>
    <dbReference type="KEGG" id="mig:Metig_0066"/>
    <dbReference type="HOGENOM" id="CLU_044118_5_0_2"/>
    <dbReference type="OMA" id="RWGDERY"/>
    <dbReference type="OrthoDB" id="99063at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042132">
        <property type="term" value="F:fructose 1,6-bisphosphate 1-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046855">
        <property type="term" value="P:inositol phosphate dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020583">
        <property type="entry name" value="Inositol_monoP_metal-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000760">
        <property type="entry name" value="Inositol_monophosphatase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00459">
        <property type="entry name" value="Inositol_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00377">
        <property type="entry name" value="IMPHPHTASES"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00629">
        <property type="entry name" value="IMP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00013093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEF95627.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="251" mass="28064" checksum="E83AA2D1E0CE3281" modified="2011-07-27" version="1">MKWNEIALKIAKDIEKEIMPLFGKEEASKFIGFSPSGDQTKLVDKKAEDIVLKHLLPLDVNIVSEESGNINKESEYTVVIDPVDGSYNFINGIPICGFSFAVFKKDEPIYTMIYEFVTKNVYEGIPKRGAYLNGEKIKVRPLKLESISLSFYGNQRINHIKNVKRVRILGAIAIELAYMAKGSLDGIVDIRRYIRPTDIVAGVVIAKEAGAIITDENGEELKFSLSATERLNVIAVNSKELLDIVLESIHE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEG8</accession>
    <name>F6BEG8_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Fructose-bisphosphate aldolase</fullName>
            <ecNumber evidence="2">4.1.2.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metig_0068</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DeoC/FbaB aldolase family.</text>
    </comment>
    <dbReference type="EC" id="4.1.2.13" evidence="2"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95629.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0068"/>
    <dbReference type="EnsemblBacteria" id="AEF95629">
        <property type="protein sequence ID" value="AEF95629"/>
        <property type="gene ID" value="Metig_0068"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0068"/>
    <dbReference type="HOGENOM" id="CLU_057069_2_2_2"/>
    <dbReference type="OMA" id="FVKVNYP"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004332">
        <property type="term" value="F:fructose-bisphosphate aldolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd00958">
        <property type="entry name" value="DhnA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002915">
        <property type="entry name" value="DeoC/FbaB/LacD_aldolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041720">
        <property type="entry name" value="FbaB-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01791">
        <property type="entry name" value="DeoC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038992">
        <property type="entry name" value="Aldolase_Ia"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01133">
        <property type="entry name" value="DeoC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0704">Schiff base</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEF95629.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="301" mass="33158" checksum="5AAED178EAA0F78B" modified="2011-07-27" version="1">MGKKDVIVPLDVPESARKKYIENYLELTKNTGRVMLFAGDQKMEHLNDDFYGEGIAKDDADPEHLFRIASKAKIGAFATQLGLIARYGMDYRKVPYIVKINSKTHLVKTSQRDPISRALIDVKDVVEFQNNSGLNILGVGYTIYPGSEYEHIMFAEASRIVYEAHKHGLIAIIWSYPRGKAVKNEKDPHLIAGAAGVAACLGADFVKVNYPECKNAAEAFKEAVLAAGRTKVLCAGGPSVDVKAFLQRLYEQIHVSGAAGNATGRNIHQKPLDEAIRMCNAIYAITVENKTVEEALKIYEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEL1</accession>
    <name>F6BEL1_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Bacteriohemerythrin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Metig_0111</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Oxygen-binding protein. May be involved in a storage mechanism or for delivery to oxygen-requiring enzymes. The oxygen-binding site contains two iron atoms.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the hemerythrin family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95672.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798281.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0111"/>
    <dbReference type="EnsemblBacteria" id="AEF95672">
        <property type="protein sequence ID" value="AEF95672"/>
        <property type="gene ID" value="Metig_0111"/>
    </dbReference>
    <dbReference type="GeneID" id="10642946"/>
    <dbReference type="KEGG" id="mig:Metig_0111"/>
    <dbReference type="HOGENOM" id="CLU_086902_2_2_2"/>
    <dbReference type="OMA" id="AKHFKHE"/>
    <dbReference type="OrthoDB" id="86598at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005344">
        <property type="term" value="F:oxygen carrier activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd12107">
        <property type="entry name" value="Hemerythrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00556">
        <property type="entry name" value="Hemerythrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023504">
        <property type="entry name" value="Bacteriohemerythrin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016131">
        <property type="entry name" value="Haemerythrin_Fe_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012312">
        <property type="entry name" value="Hemerythrin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035938">
        <property type="entry name" value="Hemerythrin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012827">
        <property type="entry name" value="Hemerythrin_metal-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01814">
        <property type="entry name" value="Hemerythrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47188">
        <property type="entry name" value="SSF47188"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02481">
        <property type="entry name" value="hemeryth_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00550">
        <property type="entry name" value="HEMERYTHRINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0408">Iron</keyword>
    <keyword evidence="4 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 6" id="KW-0561">Oxygen transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Hemerythrin" evidence="7">
        <location>
            <begin position="14"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="22"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="59"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="63"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="78"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="82"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="119"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010587"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022621"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEF95672.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="146" mass="17336" checksum="F29301E659F6DF3B" modified="2011-07-27" version="1">MKEIIKWDKSLETGIRAFDEEHKFLVKTLNEIYNLLEKGNKDEAKNILKKRVVEYAAKHFKHEEEIMEKYGYPNLERHKKTHEIFVKTVIEKLLPEIEKGSEEDFRSALSFLVGWLTMHIGKPDKDYGEWFKEKGITIDEDPIIID</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEM1</accession>
    <name>F6BEM1_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">PHP domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1281</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96818.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1281"/>
    <dbReference type="EnsemblBacteria" id="AEF96818">
        <property type="protein sequence ID" value="AEF96818"/>
        <property type="gene ID" value="Metig_1281"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_1281"/>
    <dbReference type="HOGENOM" id="CLU_106253_0_0_2"/>
    <dbReference type="OMA" id="GHNITNG"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004013">
        <property type="entry name" value="PHP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003141">
        <property type="entry name" value="Pol/His_phosphatase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016195">
        <property type="entry name" value="Pol/histidinol_Pase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02811">
        <property type="entry name" value="PHP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00481">
        <property type="entry name" value="POLIIIAc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89550">
        <property type="entry name" value="SSF89550"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="POLIIIAc" evidence="1">
        <location>
            <begin position="8"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96818.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="223" mass="25013" checksum="D8B7EBF0BA473729" modified="2011-07-27" version="1">MIGDKMRFDFHTHTVFSDGELIPSELVRRAIVLEHKAIAITDHADASNYKELIEKTILAKEELKKYWDIEVIVGVELTHIPPKSIPKMAKKAKDLGAEIVVVHGETVVEPVEEGTNYYASISEDVDILAHPGFIDDETAQNVKENGIFLEITSRRGHSLTNGYVLSVARKFDIPTLINTDTHAPEDLIDVEFAKKVGLGCGMSKKELENTLLHYPKELLKRIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-06-02" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEN9</accession>
    <name>F6BEN9_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1299</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96836.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799433.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96836">
        <property type="protein sequence ID" value="AEF96836"/>
        <property type="gene ID" value="Metig_1299"/>
    </dbReference>
    <dbReference type="GeneID" id="10644162"/>
    <dbReference type="KEGG" id="mig:Metig_1299"/>
    <dbReference type="HOGENOM" id="CLU_538218_0_0_2"/>
    <dbReference type="OMA" id="IPPIFRR"/>
    <dbReference type="OrthoDB" id="51931at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="292"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="309"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="338"/>
            <end position="355"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96836.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="506" mass="55725" checksum="E6C0C91BFD31C5C9" modified="2011-07-27" version="1">MKSIKILLILFTLTFPASASELTEYRVVVFYPNGTQTVYADWVLANVTERYGVNIFYSDTLQIPSSGNYTFKIPHKPFNISIFIDFNGQEVIYKIKNDYNFPLNINLNIINTTGSIRCGDHCINATLDSNDIKANFSIPPHSTGLLYIKPPALPFEVGKSYLTFNFTSPARVNITAPVTVSVEKEYNGIWHAKFEVKNLYNGSIFARIKTWYEVDGERYNLSSNFTAIEPYGEWNTSSSVESQSVPVFYIACKAVNITVWNVTINPAYPSNISNPGSKYILGKAIVMNKIIPKPPTGGGNGGGGGSLPPTPTTPTPTPTSTPTQPVKPTPPSKPTQTSTPETTPTPEQTSPTRTFPVKTPEVKETVTVTGVKINAVKLDKKTSTAIATFFTITLPLSTFLLSLIVSRPNVVDRGLFRPEEIATFGRTVYVPIKCNMGKILPGNVSFVVPDSELARDIHEYYDIPLRSAEAIAIAIKCNGRVFLSDFKAYKVALRLGLDAVYIPEML</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEP6</accession>
    <name>F6BEP6_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Ferripyochelin binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_1306</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96843.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799440.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1306"/>
    <dbReference type="EnsemblBacteria" id="AEF96843">
        <property type="protein sequence ID" value="AEF96843"/>
        <property type="gene ID" value="Metig_1306"/>
    </dbReference>
    <dbReference type="GeneID" id="10644169"/>
    <dbReference type="KEGG" id="mig:Metig_1306"/>
    <dbReference type="HOGENOM" id="CLU_064827_4_1_2"/>
    <dbReference type="OMA" id="YVTIGHN"/>
    <dbReference type="OrthoDB" id="84330at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001451">
        <property type="entry name" value="Hexapep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011004">
        <property type="entry name" value="Trimer_LpxA-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00132">
        <property type="entry name" value="Hexapep"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51161">
        <property type="entry name" value="SSF51161"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF96843.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="160" mass="17311" checksum="3616EE7C6ED5E33C" modified="2011-07-27" version="1">MKNVWIAKNATIVGDVELKEDVSVWYNAVLRGDLDKIIVGRGSNVQDNCVIHVSKGYPTIIGEYVSIGHGAVLHGCKIGNNVLVGMNTTILNGAKIGDNCIIGANALVTQNKEIPPNSLVLGVPGKVVRELSEEEIKSIKENALRYIDLAKKILIKEKII</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>F6BET5</accession>
    <name>F6BET5_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Peptidyl-prolyl cis-trans isomerase</fullName>
            <ecNumber evidence="2">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metig_1347</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 2">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FKBP-type PPIase family.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="2"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96882.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799479.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96882">
        <property type="protein sequence ID" value="AEF96882"/>
        <property type="gene ID" value="Metig_1347"/>
    </dbReference>
    <dbReference type="GeneID" id="10644210"/>
    <dbReference type="KEGG" id="mig:Metig_1347"/>
    <dbReference type="HOGENOM" id="CLU_098197_2_0_2"/>
    <dbReference type="OMA" id="FGQTEDH"/>
    <dbReference type="OrthoDB" id="91048at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046357">
        <property type="entry name" value="PPIase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001179">
        <property type="entry name" value="PPIase_FKBP_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00254">
        <property type="entry name" value="FKBP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50059">
        <property type="entry name" value="FKBP_PPIASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 2 4" id="KW-0413">Isomerase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0697">Rotamase</keyword>
    <feature type="domain" description="PPIase FKBP-type" evidence="3">
        <location>
            <begin position="5"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEF96882.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="153" mass="17057" checksum="FF8851EA6B70D830" modified="2011-07-27" version="1">MIKKGDKVKVHYIGRLVNGEIFDTSMEDVAKEEGIYNPERVYEPIEFVVGEGALIEGFEEAVIGMDIGEEKTVTIPPEKAYGERNEMLVQKVPLSAFEGADFEPEEGMMILAEGIPARITEVTDEYVTLDFNHELAGEDLVFTIIVVGKEDNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>F6BET7</accession>
    <name>F6BET7_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Energy conserving hydrogenase B integral membrane subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Metig_1349</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96884.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799481.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1349"/>
    <dbReference type="EnsemblBacteria" id="AEF96884">
        <property type="protein sequence ID" value="AEF96884"/>
        <property type="gene ID" value="Metig_1349"/>
    </dbReference>
    <dbReference type="GeneID" id="10644212"/>
    <dbReference type="KEGG" id="mig:Metig_1349"/>
    <dbReference type="HOGENOM" id="CLU_830576_0_0_2"/>
    <dbReference type="OMA" id="FPFYLAT"/>
    <dbReference type="OrthoDB" id="88886at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001694">
        <property type="entry name" value="NADH_UbQ_OxRdtase_su1/FPO"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00146">
        <property type="entry name" value="NADHdh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="66"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="109"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="149"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="185"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="237"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="271"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="303"/>
            <end position="321"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEF96884.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="322" mass="35777" checksum="65A4F401EA86C4D6" modified="2011-07-27" version="1">MIEEILSAIGVPLIAFSLSTWIPGIQRKIQARIQQRIGPSIATPGFWALFKFLYKIPKEPVAKLPNLYKALPLVSFVVLWAVFALTTLTHYHILSNEIGLVGLLKIEEMVYIIMGSLSNTVMGIRMPFIDECKGSNFLGTVKMTLEQLGAVRAFKMITIGSFPFYLATFIPFIPQHSIFLKDVVGEMFLFTLGGLFGALCYLIGYIIMLKEYPFSIMHTKADVIEGPTMEYSAKYRAFYLCVKELLMITLGSLFATLYLGIAPDINNPITIVINLAIAMLFPIIAAIVNAFTPVFTFRQVYPMSLFATTLGIIGVLCALLGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>F6BET8</accession>
    <name>F6BET8_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">NADH ubiquinone oxidoreductase 20 kDa subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1350</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96885.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799482.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1350"/>
    <dbReference type="EnsemblBacteria" id="AEF96885">
        <property type="protein sequence ID" value="AEF96885"/>
        <property type="gene ID" value="Metig_1350"/>
    </dbReference>
    <dbReference type="GeneID" id="10644213"/>
    <dbReference type="KEGG" id="mig:Metig_1350"/>
    <dbReference type="HOGENOM" id="CLU_055737_7_3_2"/>
    <dbReference type="OMA" id="WNPREAD"/>
    <dbReference type="OrthoDB" id="100307at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006137">
        <property type="entry name" value="NADH_UbQ_OxRdtase-like_20kDa"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01058">
        <property type="entry name" value="Oxidored_q6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0830">Ubiquinone</keyword>
    <feature type="domain" description="Oxidored_q6" evidence="1">
        <location>
            <begin position="22"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96885.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="148" mass="15829" checksum="BE2102030367797C" modified="2011-07-27" version="1">MSLKTLSRKNCIHVMLAYTGGCNGCDIEVVNCVLSPFYDAEQYNVLLTWNPREADILIVTGCVTKAVTKSLKKIYEEIPEPKAVVAVGACALMGGVYKHIGADLGTSSFIDGPVENVIPVDVKVPGCPPRPEDIITGIVKALPKILEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEU5</accession>
    <name>F6BEU5_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Probable membrane transporter protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Metig_0120</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the 4-toluene sulfonate uptake permease (TSUP) (TC 2.A.102) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95681.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798290.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0120"/>
    <dbReference type="EnsemblBacteria" id="AEF95681">
        <property type="protein sequence ID" value="AEF95681"/>
        <property type="gene ID" value="Metig_0120"/>
    </dbReference>
    <dbReference type="GeneID" id="10642955"/>
    <dbReference type="KEGG" id="mig:Metig_0120"/>
    <dbReference type="HOGENOM" id="CLU_045498_6_2_2"/>
    <dbReference type="OMA" id="MYHHIIN"/>
    <dbReference type="OrthoDB" id="111072at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002781">
        <property type="entry name" value="TM_pro_TauE-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01925">
        <property type="entry name" value="TauE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="6"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="45"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="79"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="109"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="139"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="179"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="211"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="247"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEF95681.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="268" mass="28768" checksum="F78E5A20C3B14358" modified="2011-07-27" version="1">MLFLIFILLGVVVGILSGLLGVGGGFIVVPALIYAFDYLNISEEFAVKMAFGTSLFVVFITSLVGAYKHSKLKNVDWKSAVIMGIMGMIGSYISGTIVVNYLSGELLRTIFGIVLIAISINMINYPKIKEVGDFVRPNLIYLLISGFLIGIFTGMVGLGGGVMAIPVMVLFLKFPIKKAIGTSLGMIILTSFGGLIPYLSADPNINLSQSLYCVGYVSLLVGLCIAIPSATFSSYGVKLSTKLDVRVLRRIFGVILFLVGLDLILNIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEV8</accession>
    <name>F6BEV8_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 5">Adenine deaminase</fullName>
            <shortName evidence="5">Adenase</shortName>
            <shortName evidence="5">Adenine aminase</shortName>
            <ecNumber evidence="3 5">3.5.4.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">ade</name>
        <name evidence="8" type="ordered locus">Metig_0134</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 5">
            <text>adenine + H(+) + H2O = hypoxanthine + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:23688"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16708"/>
            <dbReference type="ChEBI" id="CHEBI:17368"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="EC" id="3.5.4.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the metallo-dependent hydrolases superfamily. Adenine deaminase family.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.2" evidence="3 5"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95694.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0134"/>
    <dbReference type="EnsemblBacteria" id="AEF95694">
        <property type="protein sequence ID" value="AEF95694"/>
        <property type="gene ID" value="Metig_0134"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0134"/>
    <dbReference type="HOGENOM" id="CLU_027935_0_0_2"/>
    <dbReference type="OMA" id="TDHECFT"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000034">
        <property type="term" value="F:adenine deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006146">
        <property type="term" value="P:adenine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01518">
        <property type="entry name" value="Adenine_deamin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006679">
        <property type="entry name" value="Adenine_deam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026912">
        <property type="entry name" value="Adenine_deam_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006680">
        <property type="entry name" value="Amidohydro-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011059">
        <property type="entry name" value="Metal-dep_hydrolase_composite"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11113:SF2">
        <property type="entry name" value="PTHR11113:SF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13382">
        <property type="entry name" value="Adenine_deam_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01979">
        <property type="entry name" value="Amidohydro_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51338">
        <property type="entry name" value="SSF51338"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="SSF51556"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01178">
        <property type="entry name" value="ade"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0464">Manganese</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidohydro-rel" evidence="6">
        <location>
            <begin position="52"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="domain" description="Adenine_deam_C" evidence="7">
        <location>
            <begin position="387"/>
            <end position="543"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01518"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF13382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEF95694.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="552" mass="61947" checksum="E798E83C844FD7E4" modified="2011-07-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEZ8</accession>
    <name>F6BEZ8_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="6">dTDP-glucose 4,6-dehydratase</fullName>
            <ecNumber evidence="6">4.2.1.46</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Metig_0177</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </cofactor>
    </comment>
    <dbReference type="EC" id="4.2.1.46" evidence="6"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95734.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798343.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0177"/>
    <dbReference type="EnsemblBacteria" id="AEF95734">
        <property type="protein sequence ID" value="AEF95734"/>
        <property type="gene ID" value="Metig_0177"/>
    </dbReference>
    <dbReference type="GeneID" id="10643012"/>
    <dbReference type="KEGG" id="mig:Metig_0177"/>
    <dbReference type="HOGENOM" id="CLU_024851_0_0_2"/>
    <dbReference type="OMA" id="HNTHVDR"/>
    <dbReference type="OrthoDB" id="43299at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008460">
        <property type="term" value="F:dTDP-glucose 4,6-dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016539">
        <property type="term" value="P:intein-mediated protein splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009225">
        <property type="term" value="P:nucleotide-sugar metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05246">
        <property type="entry name" value="dTDP_GD_SDR_e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.28.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005888">
        <property type="entry name" value="dTDP_Gluc_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003586">
        <property type="entry name" value="Hint_dom_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003587">
        <property type="entry name" value="Hint_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036844">
        <property type="entry name" value="Hint_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027434">
        <property type="entry name" value="Homing_endonucl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006142">
        <property type="entry name" value="INTEIN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030934">
        <property type="entry name" value="Intein_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004042">
        <property type="entry name" value="Intein_endonuc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004860">
        <property type="entry name" value="LAGLIDADG_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016040">
        <property type="entry name" value="NAD(P)-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16363">
        <property type="entry name" value="GDP_Man_Dehyd"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14528">
        <property type="entry name" value="LAGLIDADG_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00379">
        <property type="entry name" value="INTEIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00305">
        <property type="entry name" value="HintC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00306">
        <property type="entry name" value="HintN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51294">
        <property type="entry name" value="SSF51294"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55608">
        <property type="entry name" value="SSF55608"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01181">
        <property type="entry name" value="dTDP_gluc_dehyt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01443">
        <property type="entry name" value="intein_Cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50818">
        <property type="entry name" value="INTEIN_C_TER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50819">
        <property type="entry name" value="INTEIN_ENDONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0068">Autocatalytic cleavage</keyword>
    <keyword evidence="6" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-0651">Protein splicing</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DOD-type homing endonuclease" evidence="5">
        <location>
            <begin position="240"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="domain" description="INTEIN_C_TER" evidence="4">
        <location>
            <begin position="421"/>
            <end position="445"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50818"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEF95734.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="694" mass="80084" checksum="7F46B0D9214E2B85" modified="2011-07-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F6BF27</accession>
    <name>F6BF27_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">4-vinyl reductase 4VR</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1362</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96897.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799494.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1362"/>
    <dbReference type="EnsemblBacteria" id="AEF96897">
        <property type="protein sequence ID" value="AEF96897"/>
        <property type="gene ID" value="Metig_1362"/>
    </dbReference>
    <dbReference type="GeneID" id="10644226"/>
    <dbReference type="KEGG" id="mig:Metig_1362"/>
    <dbReference type="HOGENOM" id="CLU_128702_0_0_2"/>
    <dbReference type="OMA" id="CWTNGYS"/>
    <dbReference type="OrthoDB" id="114615at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024096">
        <property type="entry name" value="NO_sig/Golgi_transp_ligand-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004096">
        <property type="entry name" value="V4R"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02830">
        <property type="entry name" value="V4R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00989">
        <property type="entry name" value="V4R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111126">
        <property type="entry name" value="SSF111126"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="V4R" evidence="1">
        <location>
            <begin position="96"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96897.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="161" mass="18376" checksum="C9EABAC82E192732" modified="2011-07-27" version="1">MKILQKEKIEQKERININFDDLDDVDRPTLGRSVDAVLFRVLKFSMLNYLGFSKNSKIYSAGKSFGKYLGVKSIEEMKEFFKAFGIGILEVVDENPLKIRIYECIDCSGLPNTGRPICFFEAGIIAGMLENILNESVHVNEVKCHATGHDYCEFEVRILKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>F6BF44</accession>
    <name>F6BF44_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ferredoxin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metig_1379</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Ferredoxins are iron-sulfur proteins that transfer electrons in a wide variety of metabolic reactions.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 2">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96914.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799510.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1379"/>
    <dbReference type="EnsemblBacteria" id="AEF96914">
        <property type="protein sequence ID" value="AEF96914"/>
        <property type="gene ID" value="Metig_1379"/>
    </dbReference>
    <dbReference type="GeneID" id="10644252"/>
    <dbReference type="KEGG" id="mig:Metig_1379"/>
    <dbReference type="HOGENOM" id="CLU_043374_3_3_2"/>
    <dbReference type="OMA" id="ILCRLCM"/>
    <dbReference type="OrthoDB" id="105930at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000813">
        <property type="entry name" value="7Fe_ferredoxin"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13247">
        <property type="entry name" value="Fer4_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00354">
        <property type="entry name" value="7FE8SFRDOXIN"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="2" id="KW-0249">Electron transport</keyword>
    <keyword evidence="2" id="KW-0408">Iron</keyword>
    <keyword evidence="2" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="3">
        <location>
            <begin position="4"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="3">
        <location>
            <begin position="64"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU365098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEF96914.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="150" mass="16750" checksum="57FB55ADFFE0E6CA" modified="2011-07-27" version="1">MNPKIIVLDPSKCSKCNDCINKCAEIHGISRVKKFDGIPIFCMQCETPPCKEICPVDAIYLKENIPIVNKEKCIGCGMCAIACPIGAIFVEEKVAHKCTLCLDVDRITPACIEACKDNALKLVCDESLEMIKEEKRKKLIKIISEKFEEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>F6BF47</accession>
    <name>F6BF47_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Acetyl-CoA decarbonylase/synthase complex subunit beta</fullName>
            <shortName evidence="9">ACDS complex subunit beta</shortName>
            <ecNumber evidence="9">2.3.1.169</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">ACDS complex acyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">cdhC</name>
        <name evidence="13" type="ordered locus">Metig_1382</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Part of a complex that catalyzes the reversible cleavage of acetyl-CoA, allowing autotrophic growth from CO(2). The alpha-epsilon complex generates CO from CO(2), while the beta subunit (this protein) combines the CO with CoA and a methyl group to form acetyl-CoA. The methyl group, which is incorporated into acetyl-CoA, is transferred to the beta subunit by a corrinoid iron-sulfur protein (the gamma-delta complex).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>acetyl-CoA + Co(I)-[corrinoid Fe-S protein] + H(+) = CO + CoA + methyl-Co(III)-[corrinoid Fe-S protein]</text>
            <dbReference type="Rhea" id="RHEA:45212"/>
            <dbReference type="Rhea" id="RHEA-COMP:11110"/>
            <dbReference type="Rhea" id="RHEA-COMP:11111"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17245"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:85033"/>
            <dbReference type="ChEBI" id="CHEBI:85035"/>
            <dbReference type="EC" id="2.3.1.169"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>[Ni-Fe-S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:60400"/>
        </cofactor>
        <text evidence="9">Binds 1 [Ni-Fe-S] cluster.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8 9">Monomer. The ACDS complex is made up of alpha, epsilon, beta, gamma and delta chains with a probable stoichiometry of (alpha(2)epsilon(2))(4)-beta(8)-(gamma(1)delta(1))(8).</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the CdhC family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.169" evidence="9"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96917.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1382"/>
    <dbReference type="EnsemblBacteria" id="AEF96917">
        <property type="protein sequence ID" value="AEF96917"/>
        <property type="gene ID" value="Metig_1382"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_1382"/>
    <dbReference type="HOGENOM" id="CLU_378422_0_0_2"/>
    <dbReference type="OMA" id="GPNQPIP"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016407">
        <property type="term" value="F:acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018492">
        <property type="term" value="F:carbon-monoxide dehydrogenase (acceptor) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043884">
        <property type="term" value="F:CO-methylating acetyl-CoA synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016151">
        <property type="term" value="F:nickel cation binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006084">
        <property type="term" value="P:acetyl-CoA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1650.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2030">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01138">
        <property type="entry name" value="CdhC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045822">
        <property type="entry name" value="ACS_CODH_B_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004461">
        <property type="entry name" value="CO_DH/Ac-CoA_synth_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038571">
        <property type="entry name" value="CO_DH/Ac-CoA_synth_bsu_3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023432">
        <property type="entry name" value="CO_DH/Ac-CoA_synth_bsu_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041350">
        <property type="entry name" value="CODH_A_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016099">
        <property type="entry name" value="Prismane-like_a/b-sand"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011254">
        <property type="entry name" value="Prismane-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42281">
        <property type="entry name" value="PTHR42281"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19436">
        <property type="entry name" value="ACS_CODH_B_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03598">
        <property type="entry name" value="CdhC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18537">
        <property type="entry name" value="CODH_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56821">
        <property type="entry name" value="SSF56821"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00316">
        <property type="entry name" value="cdhC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9 13" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 9" id="KW-0408">Iron</keyword>
    <keyword evidence="6 9" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 9" id="KW-0533">Nickel</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9 13" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="CODH_A_N" evidence="11">
        <location>
            <begin position="25"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="domain" description="ACS_CODH_B_C" evidence="12">
        <location>
            <begin position="449"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="7"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="470"/>
        </location>
        <ligand>
            <name>[Ni-Fe-S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:60400"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="473"/>
        </location>
        <ligand>
            <name>[Ni-Fe-S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:60400"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="559"/>
        </location>
        <ligand>
            <name>[Ni-Fe-S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:60400"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="561"/>
        </location>
        <ligand>
            <name>[Ni-Fe-S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:60400"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00025865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF18537"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF19436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AEF96917.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="730" mass="80565" checksum="E52953946CC109A3" modified="2011-07-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-06-02" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>F6BF49</accession>
    <name>F6BF49_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">4-vinyl reductase 4VR</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1384</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96919.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96919">
        <property type="protein sequence ID" value="AEF96919"/>
        <property type="gene ID" value="Metig_1384"/>
    </dbReference>
    <dbReference type="GeneID" id="10644257"/>
    <dbReference type="KEGG" id="mig:Metig_1384"/>
    <dbReference type="HOGENOM" id="CLU_128702_0_0_2"/>
    <dbReference type="OMA" id="GTTLCHF"/>
    <dbReference type="OrthoDB" id="114615at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024096">
        <property type="entry name" value="NO_sig/Golgi_transp_ligand-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004096">
        <property type="entry name" value="V4R"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02830">
        <property type="entry name" value="V4R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00989">
        <property type="entry name" value="V4R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111126">
        <property type="entry name" value="SSF111126"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="V4R" evidence="1">
        <location>
            <begin position="102"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96919.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="170" mass="19133" checksum="14A8D2BB2A219B45" modified="2011-07-27" version="1">MDKYLLMKRIDENIHNLIKNNSPKRDLGDLIPLSIFQAVRIGILTAGCGIEAIIYNIGKDIGKSVISNYVENSPTELLENFAEILKKTKIGILGIEKIDDDEMILSLRECVSCYNAPNVGTTLCHFEAGLISGTLESKLKRHVKVTETKCCGKGDDYCEFYVKIGERRYW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>F6BF66</accession>
    <name>F6BF66_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glutamine amidotransferase class-II</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1401</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96936.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799532.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1401"/>
    <dbReference type="EnsemblBacteria" id="AEF96936">
        <property type="protein sequence ID" value="AEF96936"/>
        <property type="gene ID" value="Metig_1401"/>
    </dbReference>
    <dbReference type="GeneID" id="10644274"/>
    <dbReference type="KEGG" id="mig:Metig_1401"/>
    <dbReference type="HOGENOM" id="CLU_059273_0_1_2"/>
    <dbReference type="OMA" id="YWVFAHN"/>
    <dbReference type="OrthoDB" id="44166at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006541">
        <property type="term" value="P:glutamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026869">
        <property type="entry name" value="EgtC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017932">
        <property type="entry name" value="GATase_2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13230">
        <property type="entry name" value="GATase_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="SSF56235"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51278">
        <property type="entry name" value="GATASE_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glutamine amidotransferase type-2" evidence="1">
        <location>
            <begin position="2"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96936.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="348" mass="40689" checksum="9BEFCFD564AA4588" modified="2011-07-27" version="1">MCELLGICFNKKVNVGLSLNSFKQRSNMHPNGWGIAFYPDEFVRVIKEPIKMNDALLAKCVRWINIKSKIFIAHIRKASAGKVSYVNTHPFVRKLGDKEIVFAHNGTLYDYWDLELDEFYPIGETDSEYAFCYLLSQIKNNKIEWGGEGFDALLDILYEINSCGPFNCLFSDGEYLFAYKDYEGRRNLYFLKRTPPYKPIKLIDEDYTVNLGDIKNPKEEGFIIATNPLTNENWQPFKNGELIVFKNGEMIYSNKRLSDLELKILKILRESPHRVSLRDIIKNLNDLPQNIEYDESVVKIGIRSLLDKEYIKQDGRDTVDWNDLEATFYTKPERRAEIDKKLKELRWS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>F6BF69</accession>
    <name>F6BF69_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_1404</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96939.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799535.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96939">
        <property type="protein sequence ID" value="AEF96939"/>
        <property type="gene ID" value="Metig_1404"/>
    </dbReference>
    <dbReference type="GeneID" id="10644277"/>
    <dbReference type="KEGG" id="mig:Metig_1404"/>
    <dbReference type="HOGENOM" id="CLU_712940_0_0_2"/>
    <dbReference type="OrthoDB" id="31645at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF96939.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="372" mass="43645" checksum="E2F6AE8439AC4FBC" modified="2011-07-27" version="1">MEVTNRAELVELLEVNMKRSYEDILDDTKLEPEQNIPKTYIIESNLNVKYLKNLGIQIKSLEDYRLYHIKFGEATYYLDAIDDRFWIFYSLQKSEISDKYINKLISPLKSRLDNLWLPNSILKSLLDNYTIRGVATKFIGSPVVDEESNRLSIDSLGYEAYELIKAINEISEEDLRNITNEKIKRSLKNLLKVKNYLRLSKAKIKFEDEENDEFVLEDVYYWGKFTVKGNDINKHFKIVNRVLDEYKDKLNLIESSLIDIESDNIGDKTPLLYEFKKDIEDLEAFVSYLTSLKQPFKIFGIPMKVEEDMFYISAVDLHNGDNFDLEITPWWIRMYLPKGSCGNTALRLLTNLQQTHDANTYLEVGINEEKIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-09-29" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAB4</accession>
    <name>F6BAB4_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Polysaccharide biosynthesis protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Metig_0247</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95804.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798413.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF95804">
        <property type="protein sequence ID" value="AEF95804"/>
        <property type="gene ID" value="Metig_0247"/>
    </dbReference>
    <dbReference type="GeneID" id="10643082"/>
    <dbReference type="KEGG" id="mig:Metig_0247"/>
    <dbReference type="HOGENOM" id="CLU_653173_0_0_2"/>
    <dbReference type="OMA" id="MGGVFAN"/>
    <dbReference type="OrthoDB" id="55352at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002797">
        <property type="entry name" value="Polysacc_synth"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01943">
        <property type="entry name" value="Polysacc_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="36"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="68"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="112"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="135"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="167"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="193"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="308"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="337"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="372"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="397"/>
            <end position="416"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEF95804.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="420" mass="49112" checksum="8FD34D4927DB60A4" modified="2011-07-27" version="1">MFKKQYSNLKDKIYKFSKRYSSKVGLDLPYFIKGGFWLSIGQFFGTLKGFILSIVFANFLSKEVFGQYSFIMTILSIATIFALLGMSVAVIQAVAKGYEGTYFRALKEIFKWSWLGSLFLLGFSVYEYLYGWFHLSLIFLILSSIFPFYAISGFYTTLLNGKKRFDILTKLSSLFNIISTILIVLTAYFTESVFWITIITVLIQIVINGYYSMFYVKRFITNNNIDEDSIKFGKSISLSIALSNIANNFDSMIIAYFLSFEELAIFKIVTSIPNQIKILAKTFIPMILPKIASEDLSKRDIMKHLKKFFVVVVFLIVVYLVLAPFIFKWFYPKYYDYVWLSMLFHLSFLSMLYILPYNYLIKEKEARLINKFYTYSSLILILLSFFGIYFYGLLGAIIARIFYRLLVMIITFLFFLKYCK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-06-02" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAE4</accession>
    <name>F6BAE4_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1422</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96957.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799553.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96957">
        <property type="protein sequence ID" value="AEF96957"/>
        <property type="gene ID" value="Metig_1422"/>
    </dbReference>
    <dbReference type="GeneID" id="10644295"/>
    <dbReference type="KEGG" id="mig:Metig_1422"/>
    <dbReference type="HOGENOM" id="CLU_203607_0_0_2"/>
    <dbReference type="OMA" id="ETAPYVQ"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="21"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96957.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="67" mass="7930" checksum="5461EC89B3635610" modified="2011-07-27" version="1">MDKLELVLTEALPYVSNLEYAKELILNSKDLEELKEKINNLLKEEKDITKKTDLRIILEKIEEIENE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2020-12-02" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAF5</accession>
    <name>F6BAF5_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Acylphosphatase</fullName>
            <ecNumber evidence="1">3.6.1.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metig_1433</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>an acyl phosphate + H2O = a carboxylate + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:14965"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29067"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:59918"/>
            <dbReference type="EC" id="3.6.1.7"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the acylphosphatase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.7" evidence="1"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96968.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96968">
        <property type="protein sequence ID" value="AEF96968"/>
        <property type="gene ID" value="Metig_1433"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_1433"/>
    <dbReference type="HOGENOM" id="CLU_1656874_0_0_2"/>
    <dbReference type="OMA" id="VYNHKDG"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003998">
        <property type="term" value="F:acylphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020456">
        <property type="entry name" value="Acylphosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001792">
        <property type="entry name" value="Acylphosphatase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036046">
        <property type="entry name" value="Acylphosphatase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017968">
        <property type="entry name" value="Acylphosphatase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47268">
        <property type="entry name" value="PTHR47268"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00708">
        <property type="entry name" value="Acylphosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54975">
        <property type="entry name" value="SSF54975"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00150">
        <property type="entry name" value="ACYLPHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51160">
        <property type="entry name" value="ACYLPHOSPHATASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acylphosphatase-like" evidence="3">
        <location>
            <begin position="16"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="31"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU004168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEF96968.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="151" mass="17621" checksum="672613E0289C6991" modified="2011-07-27" version="1">MLMNSWSNGDVFMPTTYELKIYGKVQHVGFRDRVENIGRGLGIDGVVYNHKDGTVRILANFDDEEIKELFKKNIKVLEKKDKLIKIEKIEEKELNAYIEFPDGINRISADDLIELNKKLDEGVKYIKLIFGEMEKMNEKLDKIVELLEKKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2020-08-12" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAF7</accession>
    <name>F6BAF7_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_1435</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96970.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96970">
        <property type="protein sequence ID" value="AEF96970"/>
        <property type="gene ID" value="Metig_1435"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_1435"/>
    <dbReference type="HOGENOM" id="CLU_1821028_0_0_2"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF96970.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="141" mass="16088" checksum="C5227F50AF709E63" modified="2011-07-27" version="1">MLFLTGCIKNNSTKSSNNESEIITIKITVLYDNPVSKNYLLPKNEPNSVSLYEYVKNESIPGASYFKKLKTFPLKKVGSNKYVAIIQLNISDLHENYTYCIGYPPLNYIDKNTCLVIKVTNNMPREINGTIHYWRSQLAIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAH8</accession>
    <name>F6BAH8_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Probable translation initiation factor IF-2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">infB</name>
        <name evidence="9" type="ordered locus">Metig_1456</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5 6 7">Function in general translation initiation by promoting the binding of the formylmethionine-tRNA to ribosomes. Seems to function along with eIF-2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6 7">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. IF-2 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96991.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799587.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1456"/>
    <dbReference type="EnsemblBacteria" id="AEF96991">
        <property type="protein sequence ID" value="AEF96991"/>
        <property type="gene ID" value="Metig_1456"/>
    </dbReference>
    <dbReference type="GeneID" id="10644329"/>
    <dbReference type="KEGG" id="mig:Metig_1456"/>
    <dbReference type="HOGENOM" id="CLU_002656_3_3_2"/>
    <dbReference type="OMA" id="FRQSKPA"/>
    <dbReference type="OrthoDB" id="17053at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10050">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00100_A">
        <property type="entry name" value="IF_2_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029459">
        <property type="entry name" value="EFTU-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004544">
        <property type="entry name" value="TF_aIF-2_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015760">
        <property type="entry name" value="TIF_IF2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023115">
        <property type="entry name" value="TIF_IF2_dom3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036925">
        <property type="entry name" value="TIF_IF2_dom3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43381">
        <property type="entry name" value="PTHR43381"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14578">
        <property type="entry name" value="GTP_EFTU_D4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11987">
        <property type="entry name" value="IF-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="SSF50447"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52156">
        <property type="entry name" value="SSF52156"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00491">
        <property type="entry name" value="aIF-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1 6 7" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="2 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3 6 7" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="8">
        <location>
            <begin position="3"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="12"/>
            <end position="19"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="76"/>
            <end position="80"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="130"/>
            <end position="133"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00024852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AEF96991.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="597" mass="66348" checksum="2F85745DF01C5E33" modified="2011-07-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAJ7</accession>
    <name>F6BAJ7_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Putative nickel-responsive regulator</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">Metig_1476</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 8">Transcriptional regulator.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
        </cofactor>
        <text evidence="8">Binds 1 nickel ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the transcriptional regulatory CopG/NikR family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97010.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799606.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1476"/>
    <dbReference type="EnsemblBacteria" id="AEF97010">
        <property type="protein sequence ID" value="AEF97010"/>
        <property type="gene ID" value="Metig_1476"/>
    </dbReference>
    <dbReference type="GeneID" id="10644349"/>
    <dbReference type="KEGG" id="mig:Metig_1476"/>
    <dbReference type="HOGENOM" id="CLU_113319_1_2_2"/>
    <dbReference type="OMA" id="AHNCLET"/>
    <dbReference type="OrthoDB" id="90041at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016151">
        <property type="term" value="F:nickel cation binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010045">
        <property type="term" value="P:response to nickel cation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1220.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00476">
        <property type="entry name" value="NikR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027271">
        <property type="entry name" value="Acetolactate_synth/TF_NikR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013321">
        <property type="entry name" value="Arc_rbn_hlx_hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002145">
        <property type="entry name" value="CopG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022988">
        <property type="entry name" value="Ni_resp_reg_NikR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010985">
        <property type="entry name" value="Ribbon_hlx_hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014864">
        <property type="entry name" value="TF_NikR_Ni-bd_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08753">
        <property type="entry name" value="NikR_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01402">
        <property type="entry name" value="RHH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47598">
        <property type="entry name" value="SSF47598"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="SSF55021"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 8" id="KW-0533">Nickel</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 8" id="KW-0804">Transcription</keyword>
    <keyword evidence="5 8" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="RHH_1" evidence="9">
        <location>
            <begin position="6"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="domain" description="NikR_C" evidence="10">
        <location>
            <begin position="57"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="80"/>
        </location>
        <ligand>
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="91"/>
        </location>
        <ligand>
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="93"/>
        </location>
        <ligand>
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="99"/>
        </location>
        <ligand>
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF08753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AEF97010.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="141" mass="16094" checksum="7AFF2861752256CE" modified="2011-07-27" version="1">MVDMDRISISLPTKLLNEFDEIIAERGYASRSEAIRDAIRDYIIKHKWIHSLEGERAGTINVIYDHHMSDVTEKLTEIQHDYTDIIVATLHVHLDHDHCMEVILVRGDAKQIKELTDKLTAQKGVKQVKLTVMVPGGKVPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAQ2</accession>
    <name>F6BAQ2_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Redox-active disulfide protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metig_0310</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glutaredoxin family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95866.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0310"/>
    <dbReference type="EnsemblBacteria" id="AEF95866">
        <property type="protein sequence ID" value="AEF95866"/>
        <property type="gene ID" value="Metig_0310"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0310"/>
    <dbReference type="HOGENOM" id="CLU_090389_20_2_2"/>
    <dbReference type="OMA" id="YGIMAVP"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004362">
        <property type="term" value="F:glutathione-disulfide reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045454">
        <property type="term" value="P:cell redox homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011767">
        <property type="entry name" value="GLR_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004502">
        <property type="entry name" value="Thio_glut"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012336">
        <property type="entry name" value="Thioredoxin-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13192">
        <property type="entry name" value="Thioredoxin_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00411">
        <property type="entry name" value="redox_disulf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00195">
        <property type="entry name" value="GLUTAREDOXIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0249">Electron transport</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Thioredoxin" evidence="3">
        <location>
            <begin position="1"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEF95866.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="86" mass="9438" checksum="20AB0B08CA1D0E8C" modified="2011-07-27" version="1">MGVIMVKVEVFTSPMCPHCPAAKRVVEEVAKEVSGIEVEHVDVMKEPERAAKYGIMAVPTIVIDGEVTFIGAPTKEALIEEIKKRM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAU4</accession>
    <name>F6BAU4_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Thermosome</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Metig_0352</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the TCP-1 chaperonin family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95908.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798517.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0352"/>
    <dbReference type="EnsemblBacteria" id="AEF95908">
        <property type="protein sequence ID" value="AEF95908"/>
        <property type="gene ID" value="Metig_0352"/>
    </dbReference>
    <dbReference type="GeneID" id="10643189"/>
    <dbReference type="KEGG" id="mig:Metig_0352"/>
    <dbReference type="HOGENOM" id="CLU_008891_7_3_2"/>
    <dbReference type="OMA" id="HPAANMI"/>
    <dbReference type="OrthoDB" id="11742at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03343">
        <property type="entry name" value="cpn60"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.560.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.7.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017998">
        <property type="entry name" value="Chaperone_TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002194">
        <property type="entry name" value="Chaperonin_TCP-1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002423">
        <property type="entry name" value="Cpn60/GroEL/TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027409">
        <property type="entry name" value="GroEL-like_apical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027413">
        <property type="entry name" value="GROEL-like_equatorial_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027410">
        <property type="entry name" value="TCP-1-like_intermed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012714">
        <property type="entry name" value="Thermosome_arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11353">
        <property type="entry name" value="PTHR11353"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00118">
        <property type="entry name" value="Cpn60_TCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00304">
        <property type="entry name" value="TCOMPLEXTCP1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48592">
        <property type="entry name" value="SSF48592"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52029">
        <property type="entry name" value="SSF52029"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54849">
        <property type="entry name" value="SSF54849"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02339">
        <property type="entry name" value="thermosome_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00750">
        <property type="entry name" value="TCP1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00751">
        <property type="entry name" value="TCP1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00995">
        <property type="entry name" value="TCP1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0143">Chaperone</keyword>
    <keyword evidence="2 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU004187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEF95908.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="541" mass="58963" checksum="0C46587599D0D2F8" modified="2011-07-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAU5</accession>
    <name>F6BAU5_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Transcription factor CBF/NF-Y/histone domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Metig_1498</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Binds and compact DNA (95 to 150 base pairs) to form nucleosome-like structures that contain positive DNA supercoils. Increases the resistance of DNA to thermal denaturation (in vitro).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the archaeal histone HMF family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97032.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799626.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1498"/>
    <dbReference type="EnsemblBacteria" id="AEF97032">
        <property type="protein sequence ID" value="AEF97032"/>
        <property type="gene ID" value="Metig_1498"/>
    </dbReference>
    <dbReference type="GeneID" id="10644371"/>
    <dbReference type="KEGG" id="mig:Metig_1498"/>
    <dbReference type="HOGENOM" id="CLU_2340228_0_0_2"/>
    <dbReference type="OMA" id="MKENTDM"/>
    <dbReference type="OrthoDB" id="110775at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003958">
        <property type="entry name" value="CBFA_NFYB_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00808">
        <property type="entry name" value="CBFD_NFYB_HMF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="SSF47113"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0158">Chromosome</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBFD_NFYB_HMF" evidence="7">
        <location>
            <begin position="2"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEF97032.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="97" mass="11283" checksum="8B227EEB0F630A66" modified="2011-07-27" version="1">MIPKGTIKRIMKKHTDMNISSEAVEELSNILEEIIVITTKTAEENARADNRKTIKARDIKKCDKERIREKIIELANRTEKMNILTREFLNVISSELE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAV1</accession>
    <name>F6BAV1_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1504</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97038.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1504"/>
    <dbReference type="EnsemblBacteria" id="AEF97038">
        <property type="protein sequence ID" value="AEF97038"/>
        <property type="gene ID" value="Metig_1504"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_1504"/>
    <dbReference type="HOGENOM" id="CLU_764231_0_0_2"/>
    <dbReference type="OMA" id="GPHHIFV"/>
    <dbReference type="OrthoDB" id="51573at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002760">
        <property type="entry name" value="O_anti_polymase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01901">
        <property type="entry name" value="O_anti_polymase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="42"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="95"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="131"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="199"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="241"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="317"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="343"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="367"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="390"/>
            <end position="407"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF97038.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="420" mass="47477" checksum="2DD156D2A3D68668" modified="2011-07-27" version="1">MVLKIKDFQNRSEAKSCKIQKILLNLFEKININQLEIKPNHLFLFLVCLFLVFSNVSITTSILVLLSSLFFYVSFEVGEKWGELNIKKSYNPHNIFWYKIGVFLILIGIIFTILDLIWVRGIPLFEPASRKFLNVQFTMLSHLLPLGWAIVVSSSKLNEISKNTLPKFNEISKIKHFLKDFCKFELSLFSDNTINIKRIIVYSLIFSMFIGLLGYRTQVMVLLLATAFSVYYSNKIKTKDVLLLFLLIFLVLFGLSVFRLYVLGVEGNPITSRINLTMSILDILIQNYAGYFNGVVHASIFSSWNLIPGPSSGPRTIIANSIGVGGVTITPTIFGAVVMDYGILGLIPYFGILGIIMGFCYKVAKKVKGICLGFYSIMVAYLLVGIETGILDFEVVLFYVIGFLLCLKEAFKNKITTTLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-09-29" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAV8</accession>
    <name>F6BAV8_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1511</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97045.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF97045">
        <property type="protein sequence ID" value="AEF97045"/>
        <property type="gene ID" value="Metig_1511"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_1511"/>
    <dbReference type="HOGENOM" id="CLU_2597741_0_0_2"/>
    <dbReference type="OMA" id="MDVEDYI"/>
    <dbReference type="OrthoDB" id="144069at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="5"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="27"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="55"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF97045.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="79" mass="9472" checksum="71E455286A1EE81D" modified="2011-07-27" version="1">MKYKTIFLIILVVYITILSIFVRTPDLYLNLILIGFLIEYEIFKPYFSKETQEKLNIIVYILIGIFILIVFRKIHEILS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAW3</accession>
    <name>F6BAW3_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_1516</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97050.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799644.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1516"/>
    <dbReference type="EnsemblBacteria" id="AEF97050">
        <property type="protein sequence ID" value="AEF97050"/>
        <property type="gene ID" value="Metig_1516"/>
    </dbReference>
    <dbReference type="GeneID" id="10644389"/>
    <dbReference type="KEGG" id="mig:Metig_1516"/>
    <dbReference type="HOGENOM" id="CLU_028321_0_0_2"/>
    <dbReference type="OMA" id="SDDTYVY"/>
    <dbReference type="OrthoDB" id="77279at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="444"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="121"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF97050.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="575" mass="67686" checksum="3534DCBF5EAECE42" modified="2011-07-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAW6</accession>
    <name>F6BAW6_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="4">ADP-L-glycero-D-manno-heptose-6-epimerase</fullName>
            <ecNumber evidence="4">5.1.3.20</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metig_1519</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EC" id="5.1.3.20" evidence="4"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97053.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799647.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1519"/>
    <dbReference type="EnsemblBacteria" id="AEF97053">
        <property type="protein sequence ID" value="AEF97053"/>
        <property type="gene ID" value="Metig_1519"/>
    </dbReference>
    <dbReference type="GeneID" id="10644392"/>
    <dbReference type="KEGG" id="mig:Metig_1519"/>
    <dbReference type="HOGENOM" id="CLU_007383_1_3_2"/>
    <dbReference type="OMA" id="LRDFVYI"/>
    <dbReference type="OrthoDB" id="43299at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008712">
        <property type="term" value="F:ADP-glyceromanno-heptose 6-epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011912">
        <property type="entry name" value="Heptose_epim"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02197">
        <property type="entry name" value="heptose_epim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="1 4" id="KW-0413">Isomerase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Epimerase" evidence="3">
        <location>
            <begin position="3"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEF97053.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="304" mass="34932" checksum="91F6AF0D83F72B2F" modified="2011-07-27" version="1">MKVLITGGAGFIGSNLALTLQKNYEVVVLDDFFSGHFKNLIGFEGDIISESILDVDLNQFKDVDVIFHQAAITDTTVSDQRLMMKINTEGFKRFLNFAIENNTTFIYASSAATYGNAPAPQKEEDAGKPNNIYGFSKWICDCIAKRCMEKYPDAHIVGLRYFNVFGPREQFKGKMASMVWQLMKQMLEGKRPRIFKWGEQKRDQVYVKDIVNINLLAMKAKESCIVNAGTGRAVSFNHIIEVLNSILGFEYEPEYIDNPYEEFYQNHTEADLTKAKKYLGYKPKWTFEDAVKDYVEWLKKNNYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAX8</accession>
    <name>F6BAX8_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">dCTP deaminase, dUMP-forming</fullName>
            <ecNumber evidence="2">3.5.4.30</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">Bifunctional dCTP deaminase:dUTPase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="2">DCD-DUT</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2" type="primary">dcd</name>
        <name evidence="4" type="ordered locus">Metig_1531</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Bifunctional enzyme that catalyzes both the deamination of dCTP to dUTP and the hydrolysis of dUTP to dUMP without releasing the toxic dUTP intermediate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>dCTP + 2 H2O = diphosphate + dUMP + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:19205"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61481"/>
            <dbReference type="ChEBI" id="CHEBI:246422"/>
            <dbReference type="EC" id="3.5.4.30"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Pyrimidine metabolism; dUMP biosynthesis; dUMP from dCTP: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homotrimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the dCTP deaminase family.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.30" evidence="2"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97065.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799659.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1531"/>
    <dbReference type="EnsemblBacteria" id="AEF97065">
        <property type="protein sequence ID" value="AEF97065"/>
        <property type="gene ID" value="Metig_1531"/>
    </dbReference>
    <dbReference type="GeneID" id="10644404"/>
    <dbReference type="KEGG" id="mig:Metig_1531"/>
    <dbReference type="HOGENOM" id="CLU_087476_2_1_2"/>
    <dbReference type="OMA" id="PPIERIN"/>
    <dbReference type="OrthoDB" id="91282at2157"/>
    <dbReference type="UniPathway" id="UPA00610">
        <property type="reaction ID" value="UER00667"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033973">
        <property type="term" value="F:dCTP deaminase (dUMP-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008829">
        <property type="term" value="F:dCTP deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006226">
        <property type="term" value="P:dUMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006229">
        <property type="term" value="P:dUTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07557">
        <property type="entry name" value="trimeric_dUTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00146">
        <property type="entry name" value="dCTP_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011962">
        <property type="entry name" value="dCTP_deaminase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029054">
        <property type="entry name" value="dUTPase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036157">
        <property type="entry name" value="dUTPase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033704">
        <property type="entry name" value="dUTPase_trimeric"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00692">
        <property type="entry name" value="dUTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51283">
        <property type="entry name" value="SSF51283"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02274">
        <property type="entry name" value="dCTP_deam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 2 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0546">Nucleotide metabolism</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="dUTPase" evidence="3">
        <location>
            <begin position="95"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="2">
        <location>
            <position position="145"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="117"/>
            <end position="122"/>
        </location>
        <ligand>
            <name>dCTP</name>
            <dbReference type="ChEBI" id="CHEBI:61481"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="135"/>
        </location>
        <ligand>
            <name>dCTP</name>
            <dbReference type="ChEBI" id="CHEBI:61481"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="143"/>
            <end position="145"/>
        </location>
        <ligand>
            <name>dCTP</name>
            <dbReference type="ChEBI" id="CHEBI:61481"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="163"/>
        </location>
        <ligand>
            <name>dCTP</name>
            <dbReference type="ChEBI" id="CHEBI:61481"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="177"/>
        </location>
        <ligand>
            <name>dCTP</name>
            <dbReference type="ChEBI" id="CHEBI:61481"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="184"/>
        </location>
        <ligand>
            <name>dCTP</name>
            <dbReference type="ChEBI" id="CHEBI:61481"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="188"/>
        </location>
        <ligand>
            <name>dCTP</name>
            <dbReference type="ChEBI" id="CHEBI:61481"/>
        </ligand>
    </feature>
    <feature type="site" description="Important for bifunctional activity" evidence="2">
        <location>
            <begin position="132"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEF97065.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="203" mass="23454" checksum="644FA31246F2CA1F" modified="2011-07-27" version="1">MILSDKDIFDYVKSKRIIIEPFNPDNVGPCSYDVTLGDEFIVYNDEVYDLEKELGYKRIKIKNSILVCPLNYNLTEEKEEYFKEKYNVDYVFEGGVLGTTNEYIELPNDICAQYQGRSSLGRVFLTSHQTAGWIDAGFKGKITLEIVAFDRPVILYVNQRIGQLIFSKLLSPADIGYSERKSSKYAYQKTVMPSLIYKDKQRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>F6BB15</accession>
    <name>F6BB15_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Glutamyl-tRNA(Gln) amidotransferase subunit A</fullName>
            <shortName evidence="7">Glu-ADT subunit A</shortName>
            <ecNumber evidence="7">6.3.5.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">gatA</name>
        <name evidence="9" type="ordered locus">Metig_1568</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>ATP + H2O + L-glutamine + L-glutamyl-tRNA(Gln) = ADP + H(+) + L-glutamate + L-glutaminyl-tRNA(Gln) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:17521"/>
            <dbReference type="Rhea" id="RHEA-COMP:9681"/>
            <dbReference type="Rhea" id="RHEA-COMP:9684"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:78520"/>
            <dbReference type="ChEBI" id="CHEBI:78521"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.5.7"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="7">Heterotrimer of A, B and C subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the amidase family. GatA subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.7" evidence="7"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97102.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799696.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1568"/>
    <dbReference type="EnsemblBacteria" id="AEF97102">
        <property type="protein sequence ID" value="AEF97102"/>
        <property type="gene ID" value="Metig_1568"/>
    </dbReference>
    <dbReference type="GeneID" id="10644441"/>
    <dbReference type="KEGG" id="mig:Metig_1568"/>
    <dbReference type="HOGENOM" id="CLU_009600_0_3_2"/>
    <dbReference type="OMA" id="EVSCPHF"/>
    <dbReference type="OrthoDB" id="12800at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030956">
        <property type="term" value="C:glutamyl-tRNA(Gln) amidotransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050567">
        <property type="term" value="F:glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1300.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00120">
        <property type="entry name" value="GatA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000120">
        <property type="entry name" value="Amidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020556">
        <property type="entry name" value="Amidase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023631">
        <property type="entry name" value="Amidase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036928">
        <property type="entry name" value="AS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004412">
        <property type="entry name" value="GatA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11895">
        <property type="entry name" value="PTHR11895"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01425">
        <property type="entry name" value="Amidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75304">
        <property type="entry name" value="SSF75304"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00132">
        <property type="entry name" value="gatA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00571">
        <property type="entry name" value="AMIDASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 7" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 7" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Amidase" evidence="8">
        <location>
            <begin position="5"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="7">
        <location>
            <position position="56"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="7">
        <location>
            <position position="131"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-ester intermediate" evidence="7">
        <location>
            <position position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AEF97102.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="432" mass="47756" checksum="42A43391E2B726F0" modified="2011-07-27" version="1">MIVERVQEYLEKIEKMKDINAYIEVNKERVLKEAEELEKNEKLKDKPLYGKIIAVKANINVKGYTISCASKTLENYIAPYDATVIKKLKANGALIIGMTNMDEFACGSSGETSYYGPTKNPKAEDRIPGGSSSGSAAAVAADLCDMALGSDTGGSIRNPASHCGVVGFKPSYGVVSRQGLCDLAMSFDQIGPITKNAEDALLLTNIIKGKDPSDSTTVETPKFEKEDISNYKVGVVKEFMEVADDKIRDKIEKGIEVFGDLGCEIVELSYKYVDLALPTYYLINYVEFFSATRKYDGRRYGYRIEEVCGEEVLRRILIGKHISEKEYSGKYYKRALQARNVMRKEMMRILKDVDVIVGPTVPKLPHKLGEKLSPMEMYAYDVLTVPTNICGICAGVVPCGDVNGIPVGLQIQAKPFEDQKVLSAMIAFEKAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F6BB22</accession>
    <name>F6BB22_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Molybdopterin-guanine dinucleotide biosynthesis protein B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Metig_1575</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97109.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1575"/>
    <dbReference type="EnsemblBacteria" id="AEF97109">
        <property type="protein sequence ID" value="AEF97109"/>
        <property type="gene ID" value="Metig_1575"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_1575"/>
    <dbReference type="HOGENOM" id="CLU_068199_0_1_2"/>
    <dbReference type="OMA" id="HCGYNCK"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006777">
        <property type="term" value="P:Mo-molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007202">
        <property type="entry name" value="4Fe-4S_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004435">
        <property type="entry name" value="MobB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04060">
        <property type="entry name" value="FeS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03205">
        <property type="entry name" value="MobB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00176">
        <property type="entry name" value="mobB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51656">
        <property type="entry name" value="4FE4S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S" evidence="5">
        <location>
            <begin position="138"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEF97109.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="233" mass="26010" checksum="26D93912DA35D45C" modified="2011-07-27" version="1">MFNIRCDTMRIIGVIGPKNSGKTTLICELLKKLPFKAAVIKHTCKDIDVDAEGTDSYKLKQFSNTTVFSTNKGTAFYKDKLSLEEILSKIDEDFVIVEGFKEELSKLNIPKIVMIKGNEGLELIDEHTITVIKDYDYDVEDVLKLVLEKATVPSFNLNCKHCGYDCKSFVSAVVKGEAKWNDCVLSSGIKIVVNGKIIPANPFVSKIIKNTIKAMIETLKGVDKPKNIEIKID</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>F6BB81</accession>
    <name>F6BB81_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">L-aspartate dehydrogenase</fullName>
            <ecNumber evidence="6">1.4.1.21</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">nadX</name>
        <name evidence="9" type="ordered locus">Metig_0410</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Specifically catalyzes the NAD or NADP-dependent dehydrogenation of L-aspartate to iminoaspartate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>H2O + L-aspartate + NAD(+) = H(+) + NADH + NH4(+) + oxaloacetate</text>
            <dbReference type="Rhea" id="RHEA:11788"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.4.1.21"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>H2O + L-aspartate + NADP(+) = H(+) + NADPH + NH4(+) + oxaloacetate</text>
            <dbReference type="Rhea" id="RHEA:11784"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.4.1.21"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="6">Cofactor biosynthesis; NAD(+) biosynthesis; iminoaspartate from L-aspartate (dehydrogenase route): step 1/1.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="6">The iminoaspartate product is unstable in aqueous solution and can decompose to oxaloacetate and ammonia.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the L-aspartate dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.4.1.21" evidence="6"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95966.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798575.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0410"/>
    <dbReference type="EnsemblBacteria" id="AEF95966">
        <property type="protein sequence ID" value="AEF95966"/>
        <property type="gene ID" value="Metig_0410"/>
    </dbReference>
    <dbReference type="GeneID" id="10643247"/>
    <dbReference type="KEGG" id="mig:Metig_0410"/>
    <dbReference type="HOGENOM" id="CLU_089550_0_0_2"/>
    <dbReference type="OMA" id="ECAGHSA"/>
    <dbReference type="OrthoDB" id="50102at2157"/>
    <dbReference type="UniPathway" id="UPA00253">
        <property type="reaction ID" value="UER00456"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033735">
        <property type="term" value="F:aspartate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016639">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-NH2 group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009435">
        <property type="term" value="P:NAD biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01265">
        <property type="entry name" value="NadX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005106">
        <property type="entry name" value="Asp/hSer_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002811">
        <property type="entry name" value="Asp_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022487">
        <property type="entry name" value="Asp_DH_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020626">
        <property type="entry name" value="Asp_DH_prok"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011182">
        <property type="entry name" value="L-Asp_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01958">
        <property type="entry name" value="DUF108"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03447">
        <property type="entry name" value="NAD_binding_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005227">
        <property type="entry name" value="Asp_dh_NAD_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03855">
        <property type="entry name" value="NAD_NadX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0520">NAD</keyword>
    <keyword evidence="3 6" id="KW-0521">NADP</keyword>
    <keyword evidence="4 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2 6" id="KW-0662">Pyridine nucleotide biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD_binding_3" evidence="8">
        <location>
            <begin position="8"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="domain" description="DUF108" evidence="7">
        <location>
            <begin position="167"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="218"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="124"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="190"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF03447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AEF95966.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="267" mass="28788" checksum="9F0B53B24F27406C" modified="2011-07-27" version="1">MLKIGIVGCGAIATFISKAIARKKVSNAKVVAYYDKNRHKAEKLSKITNADVCDSIDELVKKDIDLVVEAASIKAVEEVATKALSNNKDVLVMSVGAFVDKELFLKLYNLAKDKGRKIYLPSGAIAGIDAIKSLTFGKIEEVVLKTTKPVEGLKEALKDKGIDADAIKEPTVVFEGDVFDAIKQFPSNINVSVMLSIASEFPAKVIIVADPNAKTNNHEIFVKSSIGTLKVLIENVPCEENPKTSALAAYSAIRIIKDISEPIKIGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>F6BB82</accession>
    <name>F6BB82_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">PRC-barrel domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0411</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95967.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798576.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0411"/>
    <dbReference type="EnsemblBacteria" id="AEF95967">
        <property type="protein sequence ID" value="AEF95967"/>
        <property type="gene ID" value="Metig_0411"/>
    </dbReference>
    <dbReference type="GeneID" id="10643248"/>
    <dbReference type="KEGG" id="mig:Metig_0411"/>
    <dbReference type="HOGENOM" id="CLU_170070_0_0_2"/>
    <dbReference type="OMA" id="YTTTAIY"/>
    <dbReference type="OrthoDB" id="113119at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027275">
        <property type="entry name" value="PRC-brl_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011033">
        <property type="entry name" value="PRC_barrel-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05239">
        <property type="entry name" value="PRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50346">
        <property type="entry name" value="SSF50346"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PRC" evidence="1">
        <location>
            <begin position="3"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF95967.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="100" mass="10849" checksum="BE86EDEE8F5335AF" modified="2011-07-27" version="1">MAIKISDILDKQIYTTTARYVGKVYDAILDTEKSAISGIVVSDVSNGCLRDLVGDVDKKIVLPYSLITSIGNIILIKPPAKMLVKTPNAEKGKATIKQKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F6BB93</accession>
    <name>F6BB93_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="5">CoB/CoM heterodisulfide reductase, subunit C</fullName>
            <ecNumber evidence="5">1.8.98.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Metig_0422</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Cofactor metabolism; coenzyme M-coenzyme B heterodisulfide reduction; coenzyme B and coenzyme M from coenzyme M-coenzyme B heterodisulfide: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the HdrC family.</text>
    </comment>
    <dbReference type="EC" id="1.8.98.1" evidence="5"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95978.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798587.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0422"/>
    <dbReference type="EnsemblBacteria" id="AEF95978">
        <property type="protein sequence ID" value="AEF95978"/>
        <property type="gene ID" value="Metig_0422"/>
    </dbReference>
    <dbReference type="GeneID" id="10643259"/>
    <dbReference type="KEGG" id="mig:Metig_0422"/>
    <dbReference type="HOGENOM" id="CLU_121273_0_0_2"/>
    <dbReference type="OMA" id="DPDLWLC"/>
    <dbReference type="OrthoDB" id="102216at2157"/>
    <dbReference type="UniPathway" id="UPA00647">
        <property type="reaction ID" value="UER00700"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051912">
        <property type="term" value="F:CoB--CoM heterodisulfide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015948">
        <property type="term" value="P:methanogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1060.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017680">
        <property type="entry name" value="CoB/CoM_hetero-S_Rdtase_csu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009051">
        <property type="entry name" value="Helical_ferredxn"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13183">
        <property type="entry name" value="Fer4_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03290">
        <property type="entry name" value="CoB_CoM_SS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0484">Methanogenesis</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="4">
        <location>
            <begin position="26"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEF95978.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="195" mass="22267" checksum="0F40B2CE03B7F6CE" modified="2011-07-27" version="1">MLLKSEKDFDNNFVNEIIETGRTILGEGHVASFKACYQCGTCTGSCPSGRITAFKTRKLIKYAQFGMRKFAVESEDLWMCTTCYECYERCPREVKITDIIKVIRNIAAREGRMAKAHRMTALYVFKTGHAVPINEETRKARKSIGLSEVPPTTHKYPEVLEVIRGIMKDMKFCDIVGICTESMELKPVEWKDMSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>F6BB94</accession>
    <name>F6BB94_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="6">CoB/CoM heterodisulfide reductase, subunit B</fullName>
            <ecNumber evidence="6">1.8.98.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Metig_0423</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Part of a complex that catalyzes the reversible reduction of CoM-S-S-CoB to the thiol-coenzymes H-S-CoM (coenzyme M) and H-S-CoB (coenzyme B).</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Cofactor metabolism; coenzyme M-coenzyme B heterodisulfide reduction; coenzyme B and coenzyme M from coenzyme M-coenzyme B heterodisulfide: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the HdrB family.</text>
    </comment>
    <dbReference type="EC" id="1.8.98.1" evidence="6"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95979.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798588.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0423"/>
    <dbReference type="EnsemblBacteria" id="AEF95979">
        <property type="protein sequence ID" value="AEF95979"/>
        <property type="gene ID" value="Metig_0423"/>
    </dbReference>
    <dbReference type="GeneID" id="10643260"/>
    <dbReference type="KEGG" id="mig:Metig_0423"/>
    <dbReference type="HOGENOM" id="CLU_052147_1_0_2"/>
    <dbReference type="OMA" id="SCCGASH"/>
    <dbReference type="OrthoDB" id="73750at2157"/>
    <dbReference type="UniPathway" id="UPA00647">
        <property type="reaction ID" value="UER00700"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051912">
        <property type="term" value="F:CoB--CoM heterodisulfide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015948">
        <property type="term" value="P:methanogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017678">
        <property type="entry name" value="CoB/CoM_hetero-S_Rdtase_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004017">
        <property type="entry name" value="Cys_rich_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02754">
        <property type="entry name" value="CCG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03288">
        <property type="entry name" value="CoB_CoM_SS_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0484">Methanogenesis</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CCG" evidence="5">
        <location>
            <begin position="4"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="domain" description="CCG" evidence="5">
        <location>
            <begin position="147"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEF95979.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="293" mass="32310" checksum="44D06B3FA49804A1" modified="2011-07-27" version="1">MEFAFFLGCIAPNRYPGIEAATVKALKMLGIEVVDMEGASCCPAPGVFGSFDLKTWLALAARNLCVAEEMGMDIITICNGCYGSLFEANHILQENKEARDMVNKVLSKYGLEYKGTVKVRHLPEVLYYDLGVDAIAESVKKPLNINVAVHYGCHYLKPTDIKHLEGSERPKSLDELVEALGAKSIPYRDKNMCCGAGGGVRARNLDVALEMTDNKLRNMKEAGADCVTEVCPFCHLQFDRGQIEIKEKFGKEYNIPVIHYSQLLGLAMGMSPKDVALDLHFISTEPLIKKLGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-06-02" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBB4</accession>
    <name>F6BBB4_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_1588</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97121.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799714.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF97121">
        <property type="protein sequence ID" value="AEF97121"/>
        <property type="gene ID" value="Metig_1588"/>
    </dbReference>
    <dbReference type="GeneID" id="10644461"/>
    <dbReference type="KEGG" id="mig:Metig_1588"/>
    <dbReference type="HOGENOM" id="CLU_1582910_0_0_2"/>
    <dbReference type="OMA" id="KFINISY"/>
    <dbReference type="OrthoDB" id="102709at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF97121.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="168" mass="19809" checksum="C4B58D4E907834C6" modified="2011-07-27" version="1">MLTNNQKRLHYLIYLYTHNNRDSWIRQEVLFALIYFFIKNGVFDEPYDYSPSPYMWDDKPIYINISYEAMNDLDYLLDNEYIYEILLSTRGLNIPVKGYRIAKLVEYDFPNKEEIDKVLIEDGKVKPVELTDNGIVIKSKKGDDLRIDITKLGTVPYKSKSYILGVSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBB7</accession>
    <name>F6BBB7_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Methanogenesis marker protein 14</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_1591</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97124.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799717.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1591"/>
    <dbReference type="EnsemblBacteria" id="AEF97124">
        <property type="protein sequence ID" value="AEF97124"/>
        <property type="gene ID" value="Metig_1591"/>
    </dbReference>
    <dbReference type="GeneID" id="10644464"/>
    <dbReference type="KEGG" id="mig:Metig_1591"/>
    <dbReference type="HOGENOM" id="CLU_541447_0_0_2"/>
    <dbReference type="OMA" id="VMARCMN"/>
    <dbReference type="OrthoDB" id="49937at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008303">
        <property type="entry name" value="Methan_mark_14"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09887">
        <property type="entry name" value="DUF2114"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016937">
        <property type="entry name" value="UCP016937"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03285">
        <property type="entry name" value="methan_mark_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF97124.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="484" mass="52265" checksum="DE4700D5D52879BF" modified="2011-07-27" version="1">MGFLDSIRKIFKKSPKIHYAKSQTVDLIELKRNPYYIVASVELGNTTTKAIITATNMDTGKTYIVSKYVKMTRDVRKPKKGEEVFGKTLWGVELTKEAVADMVKEVLLKALEKGNLTINDLHFVVRSTGVTAGFASPEEVGAMIIALADGCLKAGVPPSKMAPAMSKNQLPKPFDEYSLMDKVIFDGAVTGVLPPTGKEVVANEMEGELVTAGIKVGSKWTEVDFRNPCMSIDFGTTLAGRITNDTLPYAKVIGNLCGLAGAIADSIARGSGLVSRDKGAALDIAKKCGDKPNKELAEEYAEKAHKYIIIDEVPPNVDRFGTVPVNPEAAKKAGTTLIGCDVGKNGSDLPKLEEIGKRIVEECNVSTLLYTLDLVSAEIAKRLVEFAYKKGLVNEKTAIGITGRAGITGEKPRLIIEKLKELDIWDNVESNVVFVEDGLALGASVMARCMNCLGTPKVPIGGNRGGGCILGLRRKWQKERGMIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-06-02" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBE4</accession>
    <name>F6BBE4_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1618</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97151.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799744.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF97151">
        <property type="protein sequence ID" value="AEF97151"/>
        <property type="gene ID" value="Metig_1618"/>
    </dbReference>
    <dbReference type="GeneID" id="10644491"/>
    <dbReference type="KEGG" id="mig:Metig_1618"/>
    <dbReference type="HOGENOM" id="CLU_2784017_0_0_2"/>
    <dbReference type="OMA" id="LICYILY"/>
    <dbReference type="OrthoDB" id="127466at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="30"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF97151.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="67" mass="7570" checksum="B21EEE900FE68F07" modified="2011-07-27" version="1">MTPEKKSGIVCLILSLIGFCILLITNSEVVTYMVFSIFAPMFIYGVGTFLIPPTRRKKEGQIPFRGW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBI2</accession>
    <name>F6BBI2_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Heavy metal transport/detoxification protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0435</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95991.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798600.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0435"/>
    <dbReference type="EnsemblBacteria" id="AEF95991">
        <property type="protein sequence ID" value="AEF95991"/>
        <property type="gene ID" value="Metig_0435"/>
    </dbReference>
    <dbReference type="GeneID" id="10643272"/>
    <dbReference type="KEGG" id="mig:Metig_0435"/>
    <dbReference type="HOGENOM" id="CLU_134973_10_3_2"/>
    <dbReference type="OMA" id="MCVKTIK"/>
    <dbReference type="OrthoDB" id="123956at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00371">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006121">
        <property type="entry name" value="HMA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036163">
        <property type="entry name" value="HMA_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00403">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55008">
        <property type="entry name" value="SSF55008"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50846">
        <property type="entry name" value="HMA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HMA" evidence="1">
        <location>
            <begin position="3"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF95991.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="71" mass="8130" checksum="99EFCF8A6483E170" modified="2011-07-27" version="1">MKELKLKIEGMSCGMCVNTIKRLLSELDGIINVDINLEEGIGIIKYDEEKINEDEILKNEAFELYPAKKIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBI4</accession>
    <name>F6BBI4_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">NADPH-dependent FMN reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0437</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95993.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798602.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0437"/>
    <dbReference type="EnsemblBacteria" id="AEF95993">
        <property type="protein sequence ID" value="AEF95993"/>
        <property type="gene ID" value="Metig_0437"/>
    </dbReference>
    <dbReference type="GeneID" id="10643274"/>
    <dbReference type="KEGG" id="mig:Metig_0437"/>
    <dbReference type="HOGENOM" id="CLU_050993_3_0_2"/>
    <dbReference type="OMA" id="NLGATFW"/>
    <dbReference type="OrthoDB" id="89591at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029039">
        <property type="entry name" value="Flavoprotein-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005025">
        <property type="entry name" value="FMN_Rdtase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03358">
        <property type="entry name" value="FMN_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52218">
        <property type="entry name" value="SSF52218"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FMN_red" evidence="1">
        <location>
            <begin position="1"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF95993.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="195" mass="21938" checksum="016A4DEEA2A7FAFE" modified="2011-07-27" version="1">MKVFGISGSPRLQGTHFAVNYALEYLKEKGVEVRYFSVSRKTINFCIHCDYCVKKKEGCIYKDDMEEVYENLIWADGVIIGTPVYQGNVSGQLKTLMDRCRAILAKNPKVLKDKIGMGIAIGGDRNGGQEIALRTIHDFYIINEMIPVGGGSFGANLGATFWSKDKGKKGIEEDEEGLRVLRKTLNRFYEVLKNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBJ5</accession>
    <name>F6BBJ5_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0285 protein Metig_0448</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0448</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0285 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96004.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798613.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0448"/>
    <dbReference type="EnsemblBacteria" id="AEF96004">
        <property type="protein sequence ID" value="AEF96004"/>
        <property type="gene ID" value="Metig_0448"/>
    </dbReference>
    <dbReference type="GeneID" id="10643285"/>
    <dbReference type="KEGG" id="mig:Metig_0448"/>
    <dbReference type="HOGENOM" id="CLU_846254_0_0_2"/>
    <dbReference type="OMA" id="CYSMGDG"/>
    <dbReference type="OrthoDB" id="33713at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01087">
        <property type="entry name" value="UPF0285"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016735">
        <property type="entry name" value="UPF0285_methan"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018783">
        <property type="entry name" value="UCP018783"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="SSF53067"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03281">
        <property type="entry name" value="methan_mark_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96004.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="325" mass="35419" checksum="88E886381C7CB48A" modified="2011-07-27" version="1">MITVGIDHGTSGITTCIKDGNEKIIFKLRRTELKNKSYIEELKKHVDLEDIDLMAVTYSMGDGINKILPIEKVKNRGVVNIEGVGEKVGGGTKVYDEIKNANIPAVVIPGLHRGIECMDERFRALYSHIASPEKISIAYNAYKTFGFEDFILSDISSNTVTLLIKDGKIFGGFDACIGAIGMLHGPLDLELIRKIDSGEITANEAFSKAGAVKIAKLYKGVEDTKMAIINNYFNDKNCKLAVDSLVLSVAMEINSLMFLNKSKNVVLAGSIGTLKHPINIPKMIKEYVDGNIFVLSGESGAVGSAMIAEDILKGKRDILGIEVEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBQ2</accession>
    <name>F6BBQ2_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0505</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96061.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0505"/>
    <dbReference type="EnsemblBacteria" id="AEF96061">
        <property type="protein sequence ID" value="AEF96061"/>
        <property type="gene ID" value="Metig_0505"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0505"/>
    <dbReference type="HOGENOM" id="CLU_1080143_0_0_2"/>
    <dbReference type="OMA" id="SEDIIMF"/>
    <dbReference type="OrthoDB" id="115057at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96061.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="257" mass="29669" checksum="E0DEAABD6A85FE3C" modified="2011-07-27" version="1">MGLFGKLKTKPNIQETPPNTPQQEPENPQNTEIQETKKEKTLNPIKILKKKKIDKEKENTALVALAPYKVKQKIIIWNTGRGEKIYGYPVAMIPHPNGMYVILYRRRCMDWLEELTVTIKSLITGKKEQYRAVHVPEECLDISEDIITIYAHSFAIVDNHTEIAIPLAKDPRERLAYLLAHQEAEMYRNALEKIGYELTNVIDSALNLNPTMRAYIGKETMSDKKKKTSKTKEFAGFDMSFSFDKFLKSLGDYDERE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBQ3</accession>
    <name>F6BBQ3_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0506</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96062.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96062">
        <property type="protein sequence ID" value="AEF96062"/>
        <property type="gene ID" value="Metig_0506"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0506"/>
    <dbReference type="HOGENOM" id="CLU_2140236_0_0_2"/>
    <dbReference type="OMA" id="ITYQDRI"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="10"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="27"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96062.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="109" mass="12865" checksum="8E782A38359E1A44" modified="2011-07-27" version="1">MMNENENFEGFEDISFEEEDIKIPDFGDDEPTEKPENTQPERKPETTPVQEEKLINFDELYKKIMIRIGERLKIIHNITYQDRIKILTVEILNDLELLNELIKAKLGGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBR5</accession>
    <name>F6BBR5_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Precorrin-6x reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metig_1663</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; adenosylcobalamin biosynthesis.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97195.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799786.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1663"/>
    <dbReference type="EnsemblBacteria" id="AEF97195">
        <property type="protein sequence ID" value="AEF97195"/>
        <property type="gene ID" value="Metig_1663"/>
    </dbReference>
    <dbReference type="GeneID" id="10644536"/>
    <dbReference type="KEGG" id="mig:Metig_1663"/>
    <dbReference type="HOGENOM" id="CLU_068627_0_0_2"/>
    <dbReference type="OMA" id="THPFAIN"/>
    <dbReference type="OrthoDB" id="94583at2157"/>
    <dbReference type="UniPathway" id="UPA00148"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016994">
        <property type="term" value="F:precorrin-6A reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003723">
        <property type="entry name" value="Precorrin-6x_reduct"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36925">
        <property type="entry name" value="PTHR36925"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02571">
        <property type="entry name" value="CbiJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00715">
        <property type="entry name" value="precor6x_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51014">
        <property type="entry name" value="COBK_CBIJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEF97195.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="254" mass="28301" checksum="3F1BBF9508FE1252" modified="2011-07-27" version="1">MKRILIMGGTSDANNIAKKLKELGNLFLITTTTTDFGGDIAKKYADVVISKPLDKDALKNVILNYSVDLLIDATHPFAVIASKNAVEVAKELNINYIRYERPSEIFKHEKIVYVKDFEEAAKKALEISNKKVFHMAGIKNLKTVVDIVGKDRVIARILPISINKALELLPPQNIVAMQGVFSKELNKYLILDYNCDVIITKESGDSGGFKEKILGALEADATPIVIKRPKIDYPVVFNDIDSLIGHIQNLDKNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBT4</accession>
    <name>F6BBT4_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">GMP synthase [glutamine-hydrolyzing] subunit A</fullName>
            <ecNumber evidence="3">6.3.5.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Glutamine amidotransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">guaAA</name>
        <name evidence="5" type="ordered locus">Metig_1682</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the synthesis of GMP from XMP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>ATP + H2O + L-glutamine + XMP = AMP + diphosphate + GMP + 2 H(+) + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:11680"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57464"/>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.5.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Purine metabolism; GMP biosynthesis; GMP from XMP (L-Gln route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Heterodimer composed of a glutamine amidotransferase subunit (A) and a GMP-binding subunit (B).</text>
    </comment>
    <dbReference type="EC" id="6.3.5.2" evidence="3"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97214.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799805.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1682"/>
    <dbReference type="MEROPS" id="C26.A31"/>
    <dbReference type="EnsemblBacteria" id="AEF97214">
        <property type="protein sequence ID" value="AEF97214"/>
        <property type="gene ID" value="Metig_1682"/>
    </dbReference>
    <dbReference type="GeneID" id="10644555"/>
    <dbReference type="KEGG" id="mig:Metig_1682"/>
    <dbReference type="HOGENOM" id="CLU_014340_1_4_2"/>
    <dbReference type="OMA" id="GPDMDRI"/>
    <dbReference type="OrthoDB" id="79931at2157"/>
    <dbReference type="UniPathway" id="UPA00189">
        <property type="reaction ID" value="UER00296"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003922">
        <property type="term" value="F:GMP synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006541">
        <property type="term" value="P:glutamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01742">
        <property type="entry name" value="GATase1_GMP_Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01510">
        <property type="entry name" value="GMP_synthase_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017926">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004739">
        <property type="entry name" value="GMP_synth_GATase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023686">
        <property type="entry name" value="GMP_synthase_A"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00117">
        <property type="entry name" value="GATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="SSF52317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00888">
        <property type="entry name" value="guaA_Nterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="1 3" id="KW-0332">GMP biosynthesis</keyword>
    <keyword evidence="3 5" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2 3" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GATase" evidence="4">
        <location>
            <begin position="4"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="3">
        <location>
            <position position="76"/>
        </location>
    </feature>
    <feature type="active site" evidence="3">
        <location>
            <position position="163"/>
        </location>
    </feature>
    <feature type="active site" evidence="3">
        <location>
            <position position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEF97214.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="189" mass="21287" checksum="C1288AC966264C39" modified="2011-07-27" version="1">MIVILNNGGQYVHRIYRSLKYMGVPSKIIPNTTPLEEIEQNEEIKGIILSGGPDIEKAKNCINIALNAKLPILGICLGHQLIAKAYGGEVGRAEAEEYASTKVYVIEENDLFKNVPKEFNAWASHKDEVKKVPEDFEILAYSDICEVEAMKHKKKPIYGVQFHPEVAHTEYGSEILKNFCKVCGFKFDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBT6</accession>
    <name>F6BBT6_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Small GTP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metig_1684</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97216.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799807.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1684"/>
    <dbReference type="EnsemblBacteria" id="AEF97216">
        <property type="protein sequence ID" value="AEF97216"/>
        <property type="gene ID" value="Metig_1684"/>
    </dbReference>
    <dbReference type="GeneID" id="10644557"/>
    <dbReference type="KEGG" id="mig:Metig_1684"/>
    <dbReference type="HOGENOM" id="CLU_044997_0_0_2"/>
    <dbReference type="OMA" id="SAKHPGQ"/>
    <dbReference type="OrthoDB" id="21317at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01896">
        <property type="entry name" value="DRG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045001">
        <property type="entry name" value="DRG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031167">
        <property type="entry name" value="G_OBG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031662">
        <property type="entry name" value="GTP-binding_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004095">
        <property type="entry name" value="TGS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012676">
        <property type="entry name" value="TGS-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43127">
        <property type="entry name" value="PTHR43127"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16897">
        <property type="entry name" value="MMR_HSR1_Xtn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02824">
        <property type="entry name" value="TGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81271">
        <property type="entry name" value="SSF81271"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51710">
        <property type="entry name" value="G_OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51880">
        <property type="entry name" value="TGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="OBG-type G" evidence="2">
        <location>
            <begin position="61"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="domain" description="TGS" evidence="3">
        <location>
            <begin position="288"/>
            <end position="363"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEF97216.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="367" mass="40879" checksum="085F8DC36F752970" modified="2011-07-27" version="1">MGIEEEIRRIEEELKNTPYNKATQKHIGRLKAKLAKLREEQNRQRGGGGGHGYAVKKSGDATVAFVGFPSVGKSTLLNKLTNAHSEVGAYAFTTLKIVPGIMEYKGAKIQVLDAPGIITGASSGKGRGTEVLAAVRSADLILLVVDVFSLEHLPVIEKELFNVGIRLDQQPPDVKIVRKERGGITVNTTVPLTHLDEETIVAILNEYKIHNADVVIREDITADQLIDVIMGNRVYIPSLVVVNKIDLATEEQLKEIEEKLKDRPHVLVSGHKEINLDKLKEAIYNTLGFIKIYLKPQGKKPDFDEPLIMLRGSTVRDVCNKLHRDFVENFRYAQVWGKSAKHPGQRVGLDHVLEDEDILTIVIKRSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBU8</accession>
    <name>F6BBU8_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Nicotinamide-nucleotide adenylyltransferase</fullName>
            <ecNumber evidence="3">2.7.7.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">NAD(+) diphosphorylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="3">NAD(+) pyrophosphorylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="3">NMN adenylyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Metig_1696</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>ATP + beta-nicotinamide D-ribonucleotide + H(+) = diphosphate + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:21360"/>
            <dbReference type="ChEBI" id="CHEBI:14649"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="EC" id="2.7.7.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from nicotinamide D-ribonucleotide: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the archaeal NMN adenylyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.1" evidence="3"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97228.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799818.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1696"/>
    <dbReference type="EnsemblBacteria" id="AEF97228">
        <property type="protein sequence ID" value="AEF97228"/>
        <property type="gene ID" value="Metig_1696"/>
    </dbReference>
    <dbReference type="GeneID" id="10644569"/>
    <dbReference type="KEGG" id="mig:Metig_1696"/>
    <dbReference type="HOGENOM" id="CLU_108783_0_0_2"/>
    <dbReference type="OMA" id="NSVWVSH"/>
    <dbReference type="OrthoDB" id="90722at2157"/>
    <dbReference type="UniPathway" id="UPA00253">
        <property type="reaction ID" value="UER00600"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000309">
        <property type="term" value="F:nicotinamide-nucleotide adenylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009435">
        <property type="term" value="P:NAD biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02166">
        <property type="entry name" value="NMNAT_Archaea"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00243">
        <property type="entry name" value="NMN_adenylyltr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004821">
        <property type="entry name" value="Cyt_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006418">
        <property type="entry name" value="NMN_Atrans_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01467">
        <property type="entry name" value="CTP_transf_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01527">
        <property type="entry name" value="arch_NMN_Atrans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00125">
        <property type="entry name" value="cyt_tran_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0520">NAD</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3 5" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="3" id="KW-0662">Pyridine nucleotide biosynthesis</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="CTP_transf_like" evidence="4">
        <location>
            <begin position="5"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEF97228.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="168" mass="19691" checksum="8EED37D56D2F2836" modified="2011-07-27" version="1">MRGFIIGRFQPFHNGHLNVIKKISKEVDELIIGIGSAQKSHTLDNPFTAGERIMMITKTLKNFDFQYYVIPINDIEFNAIWVSYVEALTPPFDVVYSGNSLVRELFEERGYMVKKPEMFNRKEYSGTEIRRRMLNGEEWEPLVPKEVAEVIKEIRGVERLIRLSEKDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBV1</accession>
    <name>F6BBV1_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_1699</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97231.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799821.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1699"/>
    <dbReference type="EnsemblBacteria" id="AEF97231">
        <property type="protein sequence ID" value="AEF97231"/>
        <property type="gene ID" value="Metig_1699"/>
    </dbReference>
    <dbReference type="GeneID" id="10644572"/>
    <dbReference type="KEGG" id="mig:Metig_1699"/>
    <dbReference type="HOGENOM" id="CLU_2766087_0_0_2"/>
    <dbReference type="OrthoDB" id="145at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF97231.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="69" mass="8091" checksum="2FCF806DD7032B6B" modified="2011-07-27" version="1">MGFIQTIPIYNNKPNILPYEKTVLELFKEKINKALKENKKLSIYCAVGYFFFEGLTELIIELSQVYLNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBV2</accession>
    <name>F6BBV2_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_1700</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97232.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799822.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF97232">
        <property type="protein sequence ID" value="AEF97232"/>
        <property type="gene ID" value="Metig_1700"/>
    </dbReference>
    <dbReference type="GeneID" id="10644573"/>
    <dbReference type="KEGG" id="mig:Metig_1700"/>
    <dbReference type="HOGENOM" id="CLU_081493_2_0_2"/>
    <dbReference type="OMA" id="SQFKSKQ"/>
    <dbReference type="OrthoDB" id="86918at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF97232.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="196" mass="22943" checksum="7EF5FA6C634955E6" modified="2011-07-27" version="1">MVREDGIIVPLKRLLEKYDEEKVKNLLNNFSCSKNPDVELFLKNTAILFEKLNKSRTYLIFKKDTKDILAYFTLALSILKIVDEEISKKTLKKLDGISKEKKEFSIYLIGQLGKNDKFWDEIKGDIILQEAIDTLCNANELVGGRIILVETLNNEKLIKFYENNGFKILQKRKDKKIDKETNNEIIIEYVQMYKII</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBW0</accession>
    <name>F6BBW0_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Signal recognition particle 19 kDa protein</fullName>
            <shortName evidence="5">SRP19</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">srp19</name>
        <name evidence="6" type="ordered locus">Metig_1708</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Involved in targeting and insertion of nascent membrane proteins into the cytoplasmic membrane. Binds directly to 7S RNA and mediates binding of the 54 kDa subunit of the SRP.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Part of the signal recognition particle protein translocation system, which is composed of SRP and FtsY. Archaeal SRP consists of a 7S RNA molecule of 300 nucleotides and two protein subunits: SRP54 and SRP19.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the SRP19 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97240.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799829.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1708"/>
    <dbReference type="EnsemblBacteria" id="AEF97240">
        <property type="protein sequence ID" value="AEF97240"/>
        <property type="gene ID" value="Metig_1708"/>
    </dbReference>
    <dbReference type="GeneID" id="10644581"/>
    <dbReference type="KEGG" id="mig:Metig_1708"/>
    <dbReference type="HOGENOM" id="CLU_169299_1_0_2"/>
    <dbReference type="OMA" id="VIWPAYL"/>
    <dbReference type="OrthoDB" id="121943at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048500">
        <property type="term" value="C:signal recognition particle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008312">
        <property type="term" value="F:7S RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006614">
        <property type="term" value="P:SRP-dependent cotranslational protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.56.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00305">
        <property type="entry name" value="SRP19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002778">
        <property type="entry name" value="Signal_recog_particle_SRP19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036521">
        <property type="entry name" value="SRP19-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022938">
        <property type="entry name" value="SRP19_arc-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17453">
        <property type="entry name" value="PTHR17453"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01922">
        <property type="entry name" value="SRP19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69695">
        <property type="entry name" value="SSF69695"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3 5" id="KW-0733">Signal recognition particle</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEF97240.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="86" mass="10195" checksum="4393AB8A8A3619BA" modified="2011-07-27" version="1">MIIWPNYIDKNKSRKEGRKVPKDIAIENPKLKEIEAALKKMGYNVKVYRDKCYPREHWRICGCIEVDAKAPKLQFLKKLCEVMKSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-06-02" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBW3</accession>
    <name>F6BBW3_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1711</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97243.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF97243">
        <property type="protein sequence ID" value="AEF97243"/>
        <property type="gene ID" value="Metig_1711"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_1711"/>
    <dbReference type="HOGENOM" id="CLU_711001_0_0_2"/>
    <dbReference type="OMA" id="CEIGINY"/>
    <dbReference type="OrthoDB" id="38332at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002760">
        <property type="entry name" value="O_anti_polymase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01901">
        <property type="entry name" value="O_anti_polymase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="50"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="80"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="103"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="128"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="183"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="222"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="314"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="345"/>
            <end position="370"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF97243.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="376" mass="43462" checksum="6E2D932AFFE0E1CF" modified="2011-07-27" version="1">MNKNKYCDNMRLEITNIKIDHPFFIVFIGHLILFLFAFLYMIMFKPPYDIQNFIKVILVVLGYLIAFYLGTRYNLKIKKILMLALFILSILITYGSYAITDNIFLSFLYAIFVLGILEGYLRYRDKINFNNLFLFIGIASFLLLVSIKKGIPLFNYNIRMSIVSDPLRLISSGALTYAGIGKYFLIAFVILVLLGYKVHVLILCVSYLIYKYRKKSLNVRQMVIFGILLILFIGFMGKVILASSNQAWKLNFMELILYRAYFDIMVLEKIVNYPEILLGKITFVPNGERLIGKLLFNYSHNITSTMFGPLYFDFGVFGVVFAFILGMMSKLIYEKGDDKIYSIYAGVLLAMCEIGINYGFLIVLLALFYVSSRLRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBY5</accession>
    <name>F6BBY5_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Integrase family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_0510</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96066.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798674.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96066">
        <property type="protein sequence ID" value="AEF96066"/>
        <property type="gene ID" value="Metig_0510"/>
    </dbReference>
    <dbReference type="GeneID" id="10643347"/>
    <dbReference type="KEGG" id="mig:Metig_0510"/>
    <dbReference type="HOGENOM" id="CLU_1472098_0_0_2"/>
    <dbReference type="OrthoDB" id="100194at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.443.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011010">
        <property type="entry name" value="DNA_brk_join_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013762">
        <property type="entry name" value="Integrase-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002104">
        <property type="entry name" value="Integrase_catalytic"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00589">
        <property type="entry name" value="Phage_integrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56349">
        <property type="entry name" value="SSF56349"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51898">
        <property type="entry name" value="TYR_RECOMBINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyr recombinase" evidence="2">
        <location>
            <begin position="1"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF96066.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="183" mass="21578" checksum="32870EE97F9026A5" modified="2011-07-27" version="1">MKDNWDMELDYEEAKKQLLKLIEQYRTKKPLYQKDKIALTYLLIGLIQLRNGCRIGEAVEGLIKIIETNEKEVKVKVEKRKDNTKRKIILPKEITNNDLETVKEVIESWKGKELRRIANNASKWFLTNLKINTHSLRYAYISHLGRQQIPAQIIASITKHKNMNMIMKYTQEKIADDVLRRIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>F6BC07</accession>
    <name>F6BC07_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">PHP domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_0533</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96088.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798695.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0533"/>
    <dbReference type="EnsemblBacteria" id="AEF96088">
        <property type="protein sequence ID" value="AEF96088"/>
        <property type="gene ID" value="Metig_0533"/>
    </dbReference>
    <dbReference type="GeneID" id="10643371"/>
    <dbReference type="KEGG" id="mig:Metig_0533"/>
    <dbReference type="HOGENOM" id="CLU_072983_0_0_2"/>
    <dbReference type="OMA" id="WEVGNAY"/>
    <dbReference type="OrthoDB" id="85871at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016195">
        <property type="entry name" value="Pol/histidinol_Pase-like"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89550">
        <property type="entry name" value="SSF89550"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF96088.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="231" mass="26958" checksum="26D5E32638DC7EE4" modified="2011-07-27" version="1">MKVDMHVHTIRSKCSLNNINLLKKICLKNNITPVIADHNTLTKTDFGITGEEIATDRGEFIGLFLNEEINEKDIFEAIDKVKEQGGLVYLPHPFDERRRRSLCKFNILDNKEFIKKVDIVEVFNSRCITNEPNIRAFEYAKKYDLLIGVGSDAHFIWEIGNAYINAEPFDRDNPREFLKLLSKYKKDMEILSKNLEKNPWISPKYYAKKGSNLNILLFSKITKKIRKKFNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F6BC11</accession>
    <name>F6BC11_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Methyltransferase small</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0537</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96092.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798699.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0537"/>
    <dbReference type="EnsemblBacteria" id="AEF96092">
        <property type="protein sequence ID" value="AEF96092"/>
        <property type="gene ID" value="Metig_0537"/>
    </dbReference>
    <dbReference type="GeneID" id="10643375"/>
    <dbReference type="KEGG" id="mig:Metig_0537"/>
    <dbReference type="HOGENOM" id="CLU_018398_7_2_2"/>
    <dbReference type="OMA" id="HMIDVNE"/>
    <dbReference type="OrthoDB" id="72076at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006807">
        <property type="term" value="P:nitrogen compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071704">
        <property type="term" value="P:organic substance metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007848">
        <property type="entry name" value="Small_mtfrase_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05175">
        <property type="entry name" value="MTS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="MTS" evidence="1">
        <location>
            <begin position="26"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96092.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="197" mass="22187" checksum="5CA1EAE768187310" modified="2011-07-27" version="1">MHYFSEKPTSKHNVKIIEDIIRGKHLIFKTDSGIFSPNKVDKGTKLLAEEVEVDKDDDVLDVGCGYGVIGIALADEVNSVVMTDINRRAVKLARENVKLNKLEDLNIEIYQGDLYEKVKHKKYDVIISNPPIKAGKKVIHRIIEEGKELLKDNGSLWVVIQTKHGAKSLAKFMEEVFGNVETVTIKGGYRILKSVKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>F6BC36</accession>
    <name>F6BC36_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 6">Phosphoribosylformylglycinamidine cyclo-ligase</fullName>
            <ecNumber evidence="2 6">6.3.3.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">AIR synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">AIRS</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Phosphoribosyl-aminoimidazole synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">purM</name>
        <name evidence="9" type="ordered locus">Metig_0563</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>2-formamido-N(1)-(5-O-phospho-beta-D-ribosyl)acetamidine + ATP = 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole + ADP + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:23032"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:137981"/>
            <dbReference type="ChEBI" id="CHEBI:147287"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.3.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 6">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 2/2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the AIR synthase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.3.1" evidence="2 6"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96117.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798724.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0563"/>
    <dbReference type="EnsemblBacteria" id="AEF96117">
        <property type="protein sequence ID" value="AEF96117"/>
        <property type="gene ID" value="Metig_0563"/>
    </dbReference>
    <dbReference type="GeneID" id="10643401"/>
    <dbReference type="KEGG" id="mig:Metig_0563"/>
    <dbReference type="HOGENOM" id="CLU_047116_0_0_2"/>
    <dbReference type="OMA" id="EPLFMTD"/>
    <dbReference type="OrthoDB" id="62508at2157"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00129"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004641">
        <property type="term" value="F:phosphoribosylformylglycinamidine cyclo-ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02196">
        <property type="entry name" value="PurM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.650.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00741">
        <property type="entry name" value="AIRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010918">
        <property type="entry name" value="PurM-like_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036676">
        <property type="entry name" value="PurM-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016188">
        <property type="entry name" value="PurM-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036921">
        <property type="entry name" value="PurM-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004733">
        <property type="entry name" value="PurM_cligase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10520">
        <property type="entry name" value="PTHR10520"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00586">
        <property type="entry name" value="AIRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02769">
        <property type="entry name" value="AIRS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55326">
        <property type="entry name" value="SSF55326"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56042">
        <property type="entry name" value="SSF56042"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00878">
        <property type="entry name" value="purM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 6 9" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AIRS" evidence="7">
        <location>
            <begin position="52"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="domain" description="AIRS_C" evidence="8">
        <location>
            <begin position="166"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AEF96117.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="341" mass="37684" checksum="D0E88B76BFB2F704" modified="2011-07-27" version="1">MVTYKDAGVDIYKEDRVIKALISQITFKRDDIKPAELEGHYAGAIEFGDYYLVLCTDGVGSKMIVAEMAKKFDTVPIDMIAMNVNDAICIGAEPVALVDYLAVEDIDEEIARQIGKGLNEGAKEANINIIGGETATLPHMIKGIDLAGTVLAIVRKDQIITGKDIKPGDVIVGLRSSGIHSNGLSLARKVFFEIAGMDIHDELPHGKTIAEELLTPTRIYVRPVLEMIKKVKVKGLAHITGGGFRKLRRLNKNVAYIIDNLPEVLPIFKEIQRLGNVPDEEMFRTFNMGIGFCVIVDKEDAEKVIEIANSYNIPAFVIGRIKEEPKNKVIVKYKGKEIHLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>F6BC56</accession>
    <name>F6BC56_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized conserved protein UCP006557, signal transduction</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_0583</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96137.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798744.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0583"/>
    <dbReference type="EnsemblBacteria" id="AEF96137">
        <property type="protein sequence ID" value="AEF96137"/>
        <property type="gene ID" value="Metig_0583"/>
    </dbReference>
    <dbReference type="GeneID" id="10643421"/>
    <dbReference type="KEGG" id="mig:Metig_0583"/>
    <dbReference type="HOGENOM" id="CLU_147797_0_0_2"/>
    <dbReference type="OMA" id="TPICGPY"/>
    <dbReference type="OrthoDB" id="92122at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012029">
        <property type="entry name" value="UCP006557"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09884">
        <property type="entry name" value="DUF2111"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006557">
        <property type="entry name" value="UCP006557_sign"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF96137.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="129" mass="14101" checksum="B552093DB745247D" modified="2011-07-27" version="1">MLKEVFKNADSKDLLPLAMAIHILVNKLPVTARSKEKPGVRLEKGKVVDDNYEGYVLKLAIEKGEVLRVTPIVGPYKGLPVIVVPIKDGDEVLGAIGAVDITAGIFEEVLLLARRPELSEFLPEDAFPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>F6BC85</accession>
    <name>F6BC85_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Peptidyl-prolyl cis-trans isomerase</fullName>
            <ecNumber evidence="2">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metig_1759</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 2">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FKBP-type PPIase family.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="2"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97291.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799880.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1759"/>
    <dbReference type="EnsemblBacteria" id="AEF97291">
        <property type="protein sequence ID" value="AEF97291"/>
        <property type="gene ID" value="Metig_1759"/>
    </dbReference>
    <dbReference type="GeneID" id="10644633"/>
    <dbReference type="KEGG" id="mig:Metig_1759"/>
    <dbReference type="HOGENOM" id="CLU_073526_0_0_2"/>
    <dbReference type="OMA" id="TMNQQWM"/>
    <dbReference type="OrthoDB" id="116879at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040825">
        <property type="entry name" value="FKBP26_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046357">
        <property type="entry name" value="PPIase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001179">
        <property type="entry name" value="PPIase_FKBP_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18046">
        <property type="entry name" value="FKBP26_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00254">
        <property type="entry name" value="FKBP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50059">
        <property type="entry name" value="FKBP_PPIASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 2 4" id="KW-0413">Isomerase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0697">Rotamase</keyword>
    <feature type="domain" description="PPIase FKBP-type" evidence="3">
        <location>
            <begin position="5"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEF97291.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="235" mass="26634" checksum="26436462FDC5F35B" modified="2011-07-27" version="1">MVEKGTLVKITYDGYVNGKLFDTTNEELAKKEGIYNPRMIYGPVTIAAGEGMVLPGLDEALLEMEVGEERELVLPPEKAFGKRDPSKVRVVPMKEFQRFNIRPIVGMPVNIDGNVGKVVSINSGRVLVDFNHELAGKEVKYRLKLEAVIEEPEEIVKELVKMYMPSINTDDLKIKMTKKTVTINLPEVCSFMNNIQTIKMAIANEIIKRLDVDKVSFVETFVKKKQDKEENENKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCA7</accession>
    <name>F6BCA7_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="3">tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_1781</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97313.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799902.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1781"/>
    <dbReference type="EnsemblBacteria" id="AEF97313">
        <property type="protein sequence ID" value="AEF97313"/>
        <property type="gene ID" value="Metig_1781"/>
    </dbReference>
    <dbReference type="GeneID" id="10644658"/>
    <dbReference type="KEGG" id="mig:Metig_1781"/>
    <dbReference type="HOGENOM" id="CLU_026481_1_1_2"/>
    <dbReference type="OMA" id="CLHINLG"/>
    <dbReference type="OrthoDB" id="57500at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034227">
        <property type="term" value="P:tRNA thio-modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002098">
        <property type="term" value="P:tRNA wobble uridine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000541">
        <property type="entry name" value="Ncs6/Tuc1/Ctu1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011063">
        <property type="entry name" value="TilS/TtcA_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035107">
        <property type="entry name" value="tRNA_thiolation_TtcA_Ctu1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020554">
        <property type="entry name" value="UPF0021_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01171">
        <property type="entry name" value="ATP_bind_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004976">
        <property type="entry name" value="ATPase_YdaO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00269">
        <property type="entry name" value="TIGR00269"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01263">
        <property type="entry name" value="UPF0021"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Rhodanese" evidence="2">
        <location>
            <begin position="14"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF97313.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="303" mass="34548" checksum="55582552E1C6DDB2" modified="2011-07-27" version="1">MKCKICKGEAYIKIKYPKMALCKEHFIEYFEKKVQKTIKKYKMIDPEDKVLIAISGGKDGAVVTYVLKKLGYNVECLHINLGIGDFSKKSLEYVKKQTSYIGVKCHIIDLKELTGKSIPEVKSRKPKCSICGTAKRYIVSKFAYDNGFNVMVTGHNLDDEAAFILNNVMNWSTEYLARQGPVLPQKGKFPKKVKPLYELTEEEISAYSSAVGLEVFSEKCPFAKKAITLEYKEMLNKLEETRPGTKYRFVMGYLKNRHLFEKEIEDIPLIECKVCGMSSSGEVCSFCKTWRLKEGIDLKIGND</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCH9</accession>
    <name>F6BCH9_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Metig_0640</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMEM19 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96190.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798795.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0640"/>
    <dbReference type="EnsemblBacteria" id="AEF96190">
        <property type="protein sequence ID" value="AEF96190"/>
        <property type="gene ID" value="Metig_0640"/>
    </dbReference>
    <dbReference type="GeneID" id="10643479"/>
    <dbReference type="KEGG" id="mig:Metig_0640"/>
    <dbReference type="HOGENOM" id="CLU_036918_2_2_2"/>
    <dbReference type="OMA" id="AYGKRTF"/>
    <dbReference type="OrthoDB" id="118524at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002794">
        <property type="entry name" value="DUF92_TMEM19"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13353">
        <property type="entry name" value="PTHR13353"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01940">
        <property type="entry name" value="DUF92"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00297">
        <property type="entry name" value="TIGR00297"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="36"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="84"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="109"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="152"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="181"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEF96190.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="230" mass="24612" checksum="E6EACE6AB5DB8E69" modified="2011-07-27" version="1">MDIISKFFLAVVIVCILALLIKKSDSLDNCGICASSIMAFIIILGAGLSWLVILLSFFILGVLVSKMGYSTKKKMGLAESRRTIRNVLANGLVPLLFVIMYCFGFKLALFGYIGSIAAATSDTFSSELGVLSKETPRLITTLKKVEKGTDGGITFFGTFMGLLGAFLIGVVSYLLFKSPDLIWIATISGILGNLADSLCGAVFERRGIMNKEHVNFIATLVGGICGLLLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCK3</accession>
    <name>F6BCK3_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Bifunctional enzyme Fae/Hps</fullName>
        </recommendedName>
        <domain>
            <recommendedName>
                <fullName evidence="3">5,6,7,8-tetrahydromethanopterin hydro-lyase</fullName>
                <ecNumber evidence="3">4.2.1.147</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="3">Formaldehyde-activating enzyme</fullName>
                <shortName evidence="3">Fae</shortName>
            </alternativeName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="3">3-hexulose-6-phosphate synthase</fullName>
                <shortName evidence="3">HPS</shortName>
                <ecNumber evidence="3">4.1.2.43</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="3">D-arabino-3-hexulose-6-phosphate formaldehyde lyase</fullName>
            </alternativeName>
        </domain>
    </protein>
    <gene>
        <name evidence="3" type="primary">fae-hps</name>
        <name evidence="5" type="ordered locus">Metig_0664</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the condensation of formaldehyde with tetrahydromethanopterin (H(4)MPT) to 5,10-methylenetetrahydromethanopterin.</text>
    </comment>
    <comment type="function">
        <text evidence="3">Catalyzes the reversible formation of ribulose-5-phosphate and formaldehyde from 3-hexulose-6-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>5,6,7,8-tetrahydromethanopterin + formaldehyde = 5,10-methylenetetrahydromethanopterin + H2O</text>
            <dbReference type="Rhea" id="RHEA:24678"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16842"/>
            <dbReference type="ChEBI" id="CHEBI:57818"/>
            <dbReference type="ChEBI" id="CHEBI:58103"/>
            <dbReference type="EC" id="4.2.1.147"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>D-ribulose 5-phosphate + formaldehyde = D-arabino-hex-3-ulose 6-phosphate</text>
            <dbReference type="Rhea" id="RHEA:25201"/>
            <dbReference type="ChEBI" id="CHEBI:16842"/>
            <dbReference type="ChEBI" id="CHEBI:58121"/>
            <dbReference type="ChEBI" id="CHEBI:58542"/>
            <dbReference type="EC" id="4.1.2.43"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Carbohydrate biosynthesis; D-ribose 5-phosphate biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the C-terminal section; belongs to the HPS/KGPDC family. HPS subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the N-terminal section; belongs to the formaldehyde-activating enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.147" evidence="3"/>
    <dbReference type="EC" id="4.1.2.43" evidence="3"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96214.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798817.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0664"/>
    <dbReference type="EnsemblBacteria" id="AEF96214">
        <property type="protein sequence ID" value="AEF96214"/>
        <property type="gene ID" value="Metig_0664"/>
    </dbReference>
    <dbReference type="GeneID" id="10643504"/>
    <dbReference type="KEGG" id="mig:Metig_0664"/>
    <dbReference type="HOGENOM" id="CLU_701335_0_0_2"/>
    <dbReference type="OMA" id="LWDPPYL"/>
    <dbReference type="OrthoDB" id="24053at2157"/>
    <dbReference type="UniPathway" id="UPA00293"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016840">
        <property type="term" value="F:carbon-nitrogen lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043801">
        <property type="term" value="F:hexulose-6-phosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016836">
        <property type="term" value="F:hydro-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004590">
        <property type="term" value="F:orotidine-5'-phosphate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006207">
        <property type="term" value="P:'de novo' pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016051">
        <property type="term" value="P:carbohydrate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04726">
        <property type="entry name" value="KGPDC_HPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01268">
        <property type="entry name" value="Fae_Hps"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020868">
        <property type="entry name" value="Fae/Hps"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014826">
        <property type="entry name" value="HCHO-activating_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037075">
        <property type="entry name" value="HCHO-activating_enzyme_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041710">
        <property type="entry name" value="HPS/KGPDC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001754">
        <property type="entry name" value="OMPdeCOase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08714">
        <property type="entry name" value="Fae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00215">
        <property type="entry name" value="OMPdecase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00934">
        <property type="entry name" value="OMPdecase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="SSF51366"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="1 3 5" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="OMPdecase" evidence="4">
        <location>
            <begin position="164"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="region of interest" description="Formaldehyde-activating enzyme" evidence="3">
        <location>
            <begin position="1"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="region of interest" description="3-hexulose-6-phosphate synthase" evidence="3">
        <location>
            <begin position="152"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="78"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEF96214.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="388" mass="43688" checksum="074018B8AC1E68CF" modified="2011-07-27" version="1">MIKFGEAVFGNEVKAVINLVIGKGKEMDEAFTNALTRTPCPLFANLRPNLIVKPLTLVVPRHPISSEIQDELLNGVIQYGVAKAIADLDLEEDLKIIATVSVPDVPLTTLTKRKLFQYYYGATKLAINRALNEYPSKEKIKKEKYRALHPLVGFRDVRLERPPYLQVALDVPTIENLEFILNALPKSDRIILEAGTPLIKKFGIEVIEHIREYFDGFIVADLKTLDTGRIEVRMAFESTANAVCISGVAPKSTILKGIHECQKCGIMSYLDMMNVENPINLYNSLKLKPDVVILHRGIDEETFGVKKEFSITKEDVDAILAIAGGVSPENIDELIKNYDILIVGRAITKSRDPGRVARMFVNRLGDDIEQYRLYLDEDEEIDIYSRVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCK7</accession>
    <name>F6BCK7_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_0668</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96218.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0668"/>
    <dbReference type="EnsemblBacteria" id="AEF96218">
        <property type="protein sequence ID" value="AEF96218"/>
        <property type="gene ID" value="Metig_0668"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0668"/>
    <dbReference type="HOGENOM" id="CLU_3057122_0_0_2"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF96218.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="53" mass="5908" checksum="F309A171EF0BD91B" modified="2011-07-27" version="1">MVGIMIKIKDENVGEVGEDYVSKPMIKKDTQILAIIGIAKKLAKYLKNEKMGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCL2</accession>
    <name>F6BCL2_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">30S ribosomal protein S3Ae</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Ribosomal protein S1e</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">rps3ae</name>
        <name evidence="6" type="ordered locus">Metig_0673</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3 4">Belongs to the eukaryotic ribosomal protein eS1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96223.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798826.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0673"/>
    <dbReference type="EnsemblBacteria" id="AEF96223">
        <property type="protein sequence ID" value="AEF96223"/>
        <property type="gene ID" value="Metig_0673"/>
    </dbReference>
    <dbReference type="GeneID" id="10643513"/>
    <dbReference type="KEGG" id="mig:Metig_0673"/>
    <dbReference type="HOGENOM" id="CLU_062507_1_0_2"/>
    <dbReference type="OMA" id="GQNAYTK"/>
    <dbReference type="OrthoDB" id="96138at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00359">
        <property type="entry name" value="Ribosomal_S3Ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030838">
        <property type="entry name" value="30S_S3Ae"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001593">
        <property type="entry name" value="Ribosomal_S3Ae"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018281">
        <property type="entry name" value="Ribosomal_S3Ae_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01015">
        <property type="entry name" value="Ribosomal_S3Ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01397">
        <property type="entry name" value="Ribosomal_S3Ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01191">
        <property type="entry name" value="RIBOSOMAL_S3AE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="1 3 4" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="209"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEF96223.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="231" mass="26617" checksum="B9927A0E407257D7" modified="2011-07-27" version="1">MAKMKARTVKGRKAKDTWKTKVWYNIYTAQEFGGVLIGQTPASDPSLVIGRVAEVSLRDLTNDPTKHMIRVYFKIYGVSGTNAVSQFVGHDTTREYLKSQIRRRRSKIDAIVDVRTKDGYKIRVKAITLTAVRARARQKTAIRKKMMEIIKAMSEEKTFPEYVQAMLMGEMANRIHEECKKIFPLKSVMIYKSEVLEFGKKENEGFVEEAKEEEQKEEVKEEQKEVVEAAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCM0</accession>
    <name>F6BCM0_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 9">tRNA-splicing ligase RtcB</fullName>
            <ecNumber evidence="9">6.5.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">rtcB</name>
        <name evidence="10" type="ordered locus">Metig_0681</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>a 3'-end 2',3'-cyclophospho-ribonucleotide-RNA + a 5'-end dephospho-ribonucleoside-RNA + GTP + H2O = a ribonucleotidyl-ribonucleotide-RNA + diphosphate + GMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:68080"/>
            <dbReference type="Rhea" id="RHEA-COMP:10464"/>
            <dbReference type="Rhea" id="RHEA-COMP:13936"/>
            <dbReference type="Rhea" id="RHEA-COMP:17355"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
            <dbReference type="ChEBI" id="CHEBI:83064"/>
            <dbReference type="ChEBI" id="CHEBI:138284"/>
            <dbReference type="ChEBI" id="CHEBI:173118"/>
            <dbReference type="EC" id="6.5.1.8"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>a 3'-end 3'-phospho-ribonucleotide-RNA + a 5'-end dephospho-ribonucleoside-RNA + GTP = a ribonucleotidyl-ribonucleotide-RNA + diphosphate + GMP</text>
            <dbReference type="Rhea" id="RHEA:68076"/>
            <dbReference type="Rhea" id="RHEA-COMP:10463"/>
            <dbReference type="Rhea" id="RHEA-COMP:13936"/>
            <dbReference type="Rhea" id="RHEA-COMP:17355"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
            <dbReference type="ChEBI" id="CHEBI:83062"/>
            <dbReference type="ChEBI" id="CHEBI:138284"/>
            <dbReference type="ChEBI" id="CHEBI:173118"/>
            <dbReference type="EC" id="6.5.1.8"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8 9">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="8 9">Binds 2 manganese ions per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 9">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the RtcB family.</text>
    </comment>
    <dbReference type="EC" id="6.5.1.-" evidence="9"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96231.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798834.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0681"/>
    <dbReference type="EnsemblBacteria" id="AEF96231">
        <property type="protein sequence ID" value="AEF96231"/>
        <property type="gene ID" value="Metig_0681"/>
    </dbReference>
    <dbReference type="GeneID" id="10643521"/>
    <dbReference type="KEGG" id="mig:Metig_0681"/>
    <dbReference type="HOGENOM" id="CLU_022279_0_1_2"/>
    <dbReference type="OMA" id="QTRGVEC"/>
    <dbReference type="OrthoDB" id="22545at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008452">
        <property type="term" value="F:RNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1860.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001233">
        <property type="entry name" value="RtcB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036025">
        <property type="entry name" value="RtcB-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11118">
        <property type="entry name" value="PTHR11118"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01139">
        <property type="entry name" value="RtcB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103365">
        <property type="entry name" value="SSF103365"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01288">
        <property type="entry name" value="UPF0027"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="9" id="KW-0436">Ligase</keyword>
    <keyword evidence="8 9" id="KW-0464">Manganese</keyword>
    <keyword evidence="8 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="GMP-histidine intermediate" evidence="6">
        <location>
            <position position="403"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="95"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="202"/>
            <end position="206"/>
        </location>
        <ligand>
            <name>GMP</name>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="203"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="234"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="328"/>
            <end position="329"/>
        </location>
        <ligand>
            <name>GMP</name>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="328"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="377"/>
            <end position="380"/>
        </location>
        <ligand>
            <name>GMP</name>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="384"/>
        </location>
        <ligand>
            <name>GMP</name>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="403"/>
            <end position="406"/>
        </location>
        <ligand>
            <name>GMP</name>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="479"/>
        </location>
        <ligand>
            <name>GMP</name>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00029282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00029311"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00033766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR601233-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR601233-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR601233-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU371113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AEF96231.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="480" mass="53056" checksum="80906EC92131711B" modified="2011-07-27" version="1">MKEYLKKVSDTIYELPMDYKECMKVPGRVFLSEILLESLEDDVLEQIANVACLPGIQKYSLAMPDCHYGYGFCIGGVAAFDVKGGVISPGGVGFDINCGVRLIRTNLTKEEVKPKIRELVSEIFKNVPSGLGSKGKIRITKNEIDDVLEEGAKWAINEGYGWDEDIKFLEEHGCMRDADASLVSDSAKKRGLPQLGSLGSGNHFLEIQYVDKVFDEETAEVFGIEENQVVVMVHTGSRGLGHQICADYIRVMEKAAKKYGIKLPDRQLACAPIESEEGIEYYKAMCCGANYAWANRQMITHWVRESFEKVFKTSAEDLEMNIIYDVAHNIAKIEEHVIDGKTKKVVVHRKGATRAFGPGSELIPKEYRKVGQPVIIPGDMGTASYLMHGTEKAMEETFGSTAHGAGRTLSRAKALKLWKGKEIKAKLEKEGIIVMADSKAVIAEECPEAYKSIDLVADVCHKSGISLKVSRMKPMGVVKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCN3</accession>
    <name>F6BCN3_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Lactaldehyde dehydrogenase</fullName>
            <ecNumber evidence="4">1.2.1.22</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Metig_0694</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(S)-lactaldehyde + H2O + NAD(+) = (S)-lactate + 2 H(+) + NADH</text>
            <dbReference type="Rhea" id="RHEA:14277"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16651"/>
            <dbReference type="ChEBI" id="CHEBI:18041"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.2.1.22"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Cofactor biosynthesis; coenzyme F420 biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homotetramer.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.22" evidence="4"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96244.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798847.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0694"/>
    <dbReference type="EnsemblBacteria" id="AEF96244">
        <property type="protein sequence ID" value="AEF96244"/>
        <property type="gene ID" value="Metig_0694"/>
    </dbReference>
    <dbReference type="GeneID" id="10643534"/>
    <dbReference type="KEGG" id="mig:Metig_0694"/>
    <dbReference type="HOGENOM" id="CLU_005391_1_0_2"/>
    <dbReference type="OMA" id="PFGGEKH"/>
    <dbReference type="OrthoDB" id="42527at2157"/>
    <dbReference type="UniPathway" id="UPA00071"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008911">
        <property type="term" value="F:lactaldehyde dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="SSF53720"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldedh" evidence="6">
        <location>
            <begin position="9"/>
            <end position="459"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00019663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEF96244.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="463" mass="50796" checksum="6AF8CE5B7DBF475F" modified="2011-07-27" version="1">MFINGEWIRREDLEVINPYSLEVIDKITSCSREETKYAIEVANEHKDVMKNLSPTKRYNLLFKIAEEIKKNKKELAKIISLDAGKPIRQSIIEVDRTITTFKLSAFYARELRGEVIPSERLIFTKREPVGVVGAITPFNFPLNLVAHKVGPAFAGGNTVVLHPSSKAPLIAIELAKIIEKVLKSMDIPLGVFNLVTGRGEVVGDEIVKNENVNMVSFTGSVEVGESIAKNAGMKKITLELGGSNPLIILKDCDLEKAVNATVKSSYLNAGQVCISVGRVIVEDEIADEFIKRIVEKSESLKVGDPLDEKTDVGPLITLESAERVESLVNQSVEEGGKVLCGGKREKTLYYPTVVEIDEDNILAKVEIFGPVTPIIRVEGLDEAIEVANNTKYGLHAGVFTNDINKALMVADALEVGGVMINDSPTFRQDNMPFGGVKKSGLGREGVKYAVEEMTEIKTIVINR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCN5</accession>
    <name>F6BCN5_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Isopropylmalate/citramalate/homocitrate synthase</fullName>
            <ecNumber evidence="4">2.3.1.182</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metig_0696</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the alpha-IPM synthase/homocitrate synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.182" evidence="4"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96246.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798849.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0696"/>
    <dbReference type="EnsemblBacteria" id="AEF96246">
        <property type="protein sequence ID" value="AEF96246"/>
        <property type="gene ID" value="Metig_0696"/>
    </dbReference>
    <dbReference type="GeneID" id="10643536"/>
    <dbReference type="KEGG" id="mig:Metig_0696"/>
    <dbReference type="HOGENOM" id="CLU_022158_4_2_2"/>
    <dbReference type="OMA" id="QIEAGFP"/>
    <dbReference type="OrthoDB" id="26145at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043714">
        <property type="term" value="F:(R)-citramalate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046912">
        <property type="term" value="F:acyltransferase activity, acyl groups converted into alkyl on transfer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019752">
        <property type="term" value="P:carboxylic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044249">
        <property type="term" value="P:cellular biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901566">
        <property type="term" value="P:organonitrogen compound biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002034">
        <property type="entry name" value="AIPM/Hcit_synth_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011830">
        <property type="entry name" value="LEU1_arch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000891">
        <property type="entry name" value="PYR_CT"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00682">
        <property type="entry name" value="HMGL-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02090">
        <property type="entry name" value="LEU1_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00815">
        <property type="entry name" value="AIPM_HOMOCIT_SYNTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00816">
        <property type="entry name" value="AIPM_HOMOCIT_SYNTH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50991">
        <property type="entry name" value="PYR_CT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Pyruvate carboxyltransferase" evidence="3">
        <location>
            <begin position="18"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEF96246.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="387" mass="43345" checksum="BBE39D004C10785C" modified="2011-07-27" version="1">MEWKAVCPYNPKLKLEDIYIYDTTLRDGEQTPGVCFTKEQKLEIARKLDELGIKQIEAGFPVVSKKEAECVKAIANEGLDAEILALSRVLKEDIDRALDCDVDGIITFIATSPLHLKYKLKKSLEEVEEMGMRAIEYAKDHGLFVAFSAEDATRTPIEDLIRIHKNAEEHGADRVHIADTTGCATPQSMEFICSELKKNLKKAHIGVHCHNDFGFAVINSIYGLIGGAKAVSTTVNGIGERAGNASLEELIMALITLYDVKLDLNLEVLTELSKMVEEYSGIKLPKNKPIVGEMVFYHESGIHVDAVIENPLTYEPFLPEVIGQKRKIVLGKHSGCRAIAYKLKEMGIEVSREELCEIVKRVKETREKGIFIDDEVFKKIVEEVIRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCP7</accession>
    <name>F6BCP7_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">UBA/THIF-type NAD/FAD binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0708</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96258.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798861.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0708"/>
    <dbReference type="EnsemblBacteria" id="AEF96258">
        <property type="protein sequence ID" value="AEF96258"/>
        <property type="gene ID" value="Metig_0708"/>
    </dbReference>
    <dbReference type="GeneID" id="10643548"/>
    <dbReference type="KEGG" id="mig:Metig_0708"/>
    <dbReference type="HOGENOM" id="CLU_013325_10_4_2"/>
    <dbReference type="OMA" id="MIYDALE"/>
    <dbReference type="OrthoDB" id="86909at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008641">
        <property type="term" value="F:ubiquitin-like modifier activating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045886">
        <property type="entry name" value="ThiF/MoeB/HesA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000594">
        <property type="entry name" value="ThiF_NAD_FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035985">
        <property type="entry name" value="Ubiquitin-activating_enz"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10953">
        <property type="entry name" value="PTHR10953"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00899">
        <property type="entry name" value="ThiF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69572">
        <property type="entry name" value="SSF69572"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ThiF" evidence="1">
        <location>
            <begin position="3"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96258.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="240" mass="26706" checksum="D658E578622B23B1" modified="2011-07-27" version="1">MRYSRQILIKNFGEEGQKKLRKAKVAIVGVGGLGCAVSQYLTAAGIGELVLIDYQTVEITNLNRQILYCEDDIGRLKVEVAKERLKCLNPEVEIKTYAEKLKEEFIKDVDVVVDCLDNFEGRYLLNEICVKNKIPFVHGAVENMHGQVTTIIPYETPCLRCIFNLKDRNETLPILGFAAGTIGTIQAGEVIKLLSGVGETLKNKLLIINLANNEFNLLNLKKNPKCSVCSKLCSNGDKDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCP9</accession>
    <name>F6BCP9_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0280 protein Metig_0710</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0710</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0280 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96260.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798863.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0710"/>
    <dbReference type="EnsemblBacteria" id="AEF96260">
        <property type="protein sequence ID" value="AEF96260"/>
        <property type="gene ID" value="Metig_0710"/>
    </dbReference>
    <dbReference type="GeneID" id="10643550"/>
    <dbReference type="KEGG" id="mig:Metig_0710"/>
    <dbReference type="HOGENOM" id="CLU_074757_0_0_2"/>
    <dbReference type="OMA" id="YGVCTSS"/>
    <dbReference type="OrthoDB" id="81284at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.520.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01079">
        <property type="entry name" value="UPF0280"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003374">
        <property type="entry name" value="ApbE-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037456">
        <property type="entry name" value="MA1715-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007183">
        <property type="entry name" value="UPF0280"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006421">
        <property type="entry name" value="UCP006421"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143631">
        <property type="entry name" value="SSF143631"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96260.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="249" mass="26950" checksum="1F8CF292AD5E7DB8" modified="2011-07-27" version="1">MNLNLIEERIIIKETNMLLKVDGKKYVKLARNTILKERMQLENYILRNPIFLTTFEPIDVEEDAPKIVKLMARASKNANVGPMAAVAGTISQLVMENLLKYGSRNVIAENGGDIALRCEKDVVVGLFAGTSPLSGEVGFKLKKEKIKNGYGVCTSSGTVGHSVSFGNADAVVVFAKEASIADAAATSIGNYAVGKEDEAINRCLEKAEEIENIDGVLVVVGKYAGKIGKIPQLVKTDKKAVLSEFFEII</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCR8</accession>
    <name>F6BCR8_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="5">6-pyruvoyltetrahydropterin synthase</fullName>
            <ecNumber evidence="5">4.2.3.12</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Metig_0729</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <dbReference type="EC" id="4.2.3.12" evidence="5"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96279.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798882.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0729"/>
    <dbReference type="EnsemblBacteria" id="AEF96279">
        <property type="protein sequence ID" value="AEF96279"/>
        <property type="gene ID" value="Metig_0729"/>
    </dbReference>
    <dbReference type="GeneID" id="10643569"/>
    <dbReference type="KEGG" id="mig:Metig_0729"/>
    <dbReference type="HOGENOM" id="CLU_111016_3_0_2"/>
    <dbReference type="OMA" id="IGQGACY"/>
    <dbReference type="OrthoDB" id="104644at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003874">
        <property type="term" value="F:6-pyruvoyltetrahydropterin synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.479.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007115">
        <property type="entry name" value="6-PTP_synth/QueD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038418">
        <property type="entry name" value="6-PTP_synth/QueD_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12589">
        <property type="entry name" value="PTHR12589"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01242">
        <property type="entry name" value="PTPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0456">Lyase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEF96279.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="166" mass="18709" checksum="3ECBC82DC6DCFE6D" modified="2011-07-27" version="1">MILELNGLHAGLRFSSAHIVFGHETCGVIHGHSYYVDVRICGEPSGEFGFVCDFKVIKSIVKEICETLDHKLMIPKNHPQTEYKIDNDSIYLKYCGDGKIKEYKIPLEDVVLLPLKSTTAEELSQFFSKCIVDKLKDMGLIDNIKWVEAVVNEGIGQGACYRLNIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCT0</accession>
    <name>F6BCT0_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0743</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96291.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0743"/>
    <dbReference type="EnsemblBacteria" id="AEF96291">
        <property type="protein sequence ID" value="AEF96291"/>
        <property type="gene ID" value="Metig_0743"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0743"/>
    <dbReference type="HOGENOM" id="CLU_3003192_0_0_2"/>
    <dbReference type="OMA" id="FILNIWV"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="31"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96291.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="58" mass="7183" checksum="FC131F502FCB5C45" modified="2011-07-27" version="1">MLKRKKYYGRDPLKKIMNDPEKREKIYKMLFILNIWVWFSMFLGAIIFIVLMYYSLKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCV1</accession>
    <name>F6BCV1_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Phosphomethylpyrimidine synthase</fullName>
            <ecNumber evidence="10">4.1.99.17</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Hydroxymethylpyrimidine phosphate synthase</fullName>
            <shortName evidence="10">HMP-P synthase</shortName>
            <shortName evidence="10">HMP-phosphate synthase</shortName>
            <shortName evidence="10">HMPP synthase</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Thiamine biosynthesis protein ThiC</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">thiC</name>
        <name evidence="11" type="ordered locus">Metig_0766</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 10">Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>5-amino-1-(5-phospho-beta-D-ribosyl)imidazole + S-adenosyl-L-methionine = 4-amino-2-methyl-5-(phosphooxymethyl)pyrimidine + 5'-deoxyadenosine + CO + formate + 3 H(+) + L-methionine</text>
            <dbReference type="Rhea" id="RHEA:24840"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:17245"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:58354"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:137981"/>
            <dbReference type="EC" id="4.1.99.17"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="10">Binds 1 [4Fe-4S] cluster per subunit. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="pathway">
        <text evidence="10">Cofactor biosynthesis; thiamine diphosphate biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the ThiC family.</text>
    </comment>
    <dbReference type="EC" id="4.1.99.17" evidence="10"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96312.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798915.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0766"/>
    <dbReference type="EnsemblBacteria" id="AEF96312">
        <property type="protein sequence ID" value="AEF96312"/>
        <property type="gene ID" value="Metig_0766"/>
    </dbReference>
    <dbReference type="GeneID" id="10643606"/>
    <dbReference type="KEGG" id="mig:Metig_0766"/>
    <dbReference type="HOGENOM" id="CLU_013181_2_2_2"/>
    <dbReference type="OMA" id="TWELFRD"/>
    <dbReference type="OrthoDB" id="25235at2157"/>
    <dbReference type="UniPathway" id="UPA00060"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016830">
        <property type="term" value="F:carbon-carbon lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009228">
        <property type="term" value="P:thiamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009229">
        <property type="term" value="P:thiamine diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.540">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00089">
        <property type="entry name" value="ThiC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037509">
        <property type="entry name" value="ThiC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038521">
        <property type="entry name" value="ThiC/Bza_core_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002817">
        <property type="entry name" value="ThiC/BzaA/B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30557">
        <property type="entry name" value="PTHR30557"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01964">
        <property type="entry name" value="ThiC_Rad_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00407">
        <property type="entry name" value="phosphomethylpyrimidine_syntha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00190">
        <property type="entry name" value="thiC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 10" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="7 10" id="KW-0408">Iron</keyword>
    <keyword evidence="8 10" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="9 10" id="KW-0456">Lyase</keyword>
    <keyword evidence="4 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="6 10" id="KW-0784">Thiamine biosynthesis</keyword>
    <keyword evidence="5 10" id="KW-0862">Zinc</keyword>
    <feature type="region of interest" description="Substrate binding" evidence="10">
        <location>
            <begin position="184"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="10">
        <location>
            <begin position="225"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="65"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="94"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="123"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="162"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="264"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="268"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="291"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="332"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="408"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="411"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="415"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AEF96312.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="426" mass="47158" checksum="655C04FE305A611E" modified="2011-07-27" version="1">MTQMIDAKNGIITKEMEFVAKEEGIEADKLRRLIAKGYVVIPKNINRDTKPVGIGEGLRTKINANIGTSPDFVDIDLEVRKAKIAEKYGADAIMDLSTGGDLKKIRKAIMEATNLPIGTVPIYEVGVEAKKKYGRVVDMDEDLIFNVIERQAKEGVDFMTLHCGITKESVETLKKSGRIMGVVSRGGSFLTAYILYHGEENPLYKNFDYLLDILKEYDVTISLGDGMRPGCLADNTDRAQIHELIILGELVDRCRERGVQCMVEGPGHVPLNNIDANIKIQKSICRNAPFYVLGPIVTDLAPGYDHITSAIGGAFAAYFGANFLCYVTPSEHVRIMTEEDVKEGVIAAKIAAQAADVAKGNAKAWEKEIAMAHARKNHDWEKQFELAIDKEKPKKMREEIPSVDEKACSICGEYCALLMVEELGKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCV2</accession>
    <name>F6BCV2_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">L-cysteine desulfidase</fullName>
            <ecNumber evidence="5">4.4.1.28</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">L-cysteine desulfhydrase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">Metig_0767</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>H2O + L-cysteine = H(+) + hydrogen sulfide + NH4(+) + pyruvate</text>
            <dbReference type="Rhea" id="RHEA:24931"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29919"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="EC" id="4.4.1.28"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homotrimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the L-cysteine desulfidase family.</text>
    </comment>
    <dbReference type="EC" id="4.4.1.28" evidence="5"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96313.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798916.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0767"/>
    <dbReference type="EnsemblBacteria" id="AEF96313">
        <property type="protein sequence ID" value="AEF96313"/>
        <property type="gene ID" value="Metig_0767"/>
    </dbReference>
    <dbReference type="GeneID" id="10643607"/>
    <dbReference type="KEGG" id="mig:Metig_0767"/>
    <dbReference type="HOGENOM" id="CLU_051840_0_0_2"/>
    <dbReference type="OMA" id="GAMIPVM"/>
    <dbReference type="OrthoDB" id="27747at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005130">
        <property type="entry name" value="Ser_deHydtase-like_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021144">
        <property type="entry name" value="UPF0597"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30501">
        <property type="entry name" value="PTHR30501"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03313">
        <property type="entry name" value="SDH_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006054">
        <property type="entry name" value="UCP006054"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="8" id="KW-0408">Iron</keyword>
    <keyword evidence="9" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="10" id="KW-0456">Lyase</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SDH_alpha" evidence="12">
        <location>
            <begin position="166"/>
            <end position="377"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00015549"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00032124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF03313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AEF96313.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="384" mass="41963" checksum="77F1F667EA344C3D" modified="2011-07-27" version="1">MDKKELMTKILKKEIVKALGCTEVGLIGYTIALCKPENPYEIEKINLTLDKGTFKNAFSVGVPNTNGFGILPAVVGGLLGDAEKRLLIFDGIEYSKELEGYIKDRLKIDVVDGDIYCKVVIKTKNGETKSTVIKGSHLNTKEEDEELKNAFKSLGLSDFLEYIEDIPKEVEDIILETIETNKELAKSEYLKLGNDELSYIVEKTTSACYERMKGTNKPAMAIAGSGNMGIMATMPIIAYHEINKGKDIERLIKSLTLSALVTIYATYHSSYISSMCGCVNRGGLGALCGLCYYIHGNDTNKITEAVKSFTANLVGIICDGGKIGCALKLASGCFAVYSSLFAEVPPNNGIVGKTFEECVKNISRIGHAMKPVDDEIINILKNKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2020-06-17" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCZ6</accession>
    <name>F6BCZ6_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_0812</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96357.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96357">
        <property type="protein sequence ID" value="AEF96357"/>
        <property type="gene ID" value="Metig_0812"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0812"/>
    <dbReference type="HOGENOM" id="CLU_1674011_0_0_2"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="135"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="110"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF96357.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="157" mass="17730" checksum="744E198846761545" modified="2011-07-27" version="1">MSKVHAEWDNEGTYEFDIPDGHYYYVVFNVTNTSCKGYFYLNSLSYSIEGGSSISTFSNNGIYKLTFKNDKYYMINVYTQNNHTTMNISTKDGHHVNFLVYDENGTLVYPSQNPPTTTTTNPQNDDSSSSTKTPIPLGAIILTLIAIPLIILRKKVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCZ8</accession>
    <name>F6BCZ8_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Methyltransferase type 11</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0814</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96359.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798961.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0814"/>
    <dbReference type="EnsemblBacteria" id="AEF96359">
        <property type="protein sequence ID" value="AEF96359"/>
        <property type="gene ID" value="Metig_0814"/>
    </dbReference>
    <dbReference type="GeneID" id="10643655"/>
    <dbReference type="KEGG" id="mig:Metig_0814"/>
    <dbReference type="HOGENOM" id="CLU_096708_0_0_2"/>
    <dbReference type="OMA" id="CLDMLYE"/>
    <dbReference type="OrthoDB" id="49237at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013216">
        <property type="entry name" value="Methyltransf_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08241">
        <property type="entry name" value="Methyltransf_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransf_11" evidence="1">
        <location>
            <begin position="45"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96359.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="233" mass="27001" checksum="5EC475F6274ED912" modified="2011-07-27" version="1">MRLAKYYDALAKEYDKVYSFEKLKWMREVENTLISKEIKKEFFVLDVGCGSGEQLKKLNNAVGLDISIEMAKIAHKKADKLVVVGNAECLPFKDNTFDCVISFFGALNHVNLDKALKEMKRILKKDGILIFTVANAYDIKWILRNIKRKGIKKTIKAIKNKKGELVRFSNRKRIKVKTKFYTLKDIEDALKKHNFEVKYTFGANITNTFIDKFIYRSFLKNFGSYIGVVAKKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2020-10-07" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>F6BD73</accession>
    <name>F6BD73_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_0891</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96434.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96434">
        <property type="protein sequence ID" value="AEF96434"/>
        <property type="gene ID" value="Metig_0891"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0891"/>
    <dbReference type="HOGENOM" id="CLU_2044423_0_0_2"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF96434.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="127" mass="15161" checksum="5281B24881346FB9" modified="2011-07-27" version="1">MKGAKLNDKKIRKIITWKEKGFETSRIAKKVGVTPRRVQQIWKEYRETGEIPKIKRRGRKPKKISKEEINLVLKAVEEYKGTSPVHLEKYIERKHNIHIPHNRIYTFLNADSIYMAFQSLRVYRSII</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDA6</accession>
    <name>F6BDA6_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Anaerobic ribonucleoside-triphosphate reductase</fullName>
            <ecNumber evidence="5">1.17.4.2</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Metig_0924</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.17.4.2" evidence="5"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96467.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799069.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0924"/>
    <dbReference type="EnsemblBacteria" id="AEF96467">
        <property type="protein sequence ID" value="AEF96467"/>
        <property type="gene ID" value="Metig_0924"/>
    </dbReference>
    <dbReference type="GeneID" id="10643770"/>
    <dbReference type="KEGG" id="mig:Metig_0924"/>
    <dbReference type="HOGENOM" id="CLU_002707_0_0_2"/>
    <dbReference type="OMA" id="YSYDRVP"/>
    <dbReference type="OrthoDB" id="5218at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008998">
        <property type="term" value="F:ribonucleoside-triphosphate reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01675">
        <property type="entry name" value="RNR_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005144">
        <property type="entry name" value="ATP-cone_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012833">
        <property type="entry name" value="NrdD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008926">
        <property type="entry name" value="RNR_R1-su_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03477">
        <property type="entry name" value="ATP-cone"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13597">
        <property type="entry name" value="NRDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48168">
        <property type="entry name" value="SSF48168"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02487">
        <property type="entry name" value="NrdD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51161">
        <property type="entry name" value="ATP_CONE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-cone" evidence="4">
        <location>
            <begin position="11"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEF96467.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="762" mass="87846" checksum="52729B9D1B368B50" modified="2011-07-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDB4</accession>
    <name>F6BDB4_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="3">HisA/hisF family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_0932</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the HisA/HisF family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96475.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799077.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0932"/>
    <dbReference type="EnsemblBacteria" id="AEF96475">
        <property type="protein sequence ID" value="AEF96475"/>
        <property type="gene ID" value="Metig_0932"/>
    </dbReference>
    <dbReference type="GeneID" id="10643778"/>
    <dbReference type="KEGG" id="mig:Metig_0932"/>
    <dbReference type="HOGENOM" id="CLU_048577_2_0_2"/>
    <dbReference type="OMA" id="LWVDAGF"/>
    <dbReference type="OrthoDB" id="84942at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003949">
        <property type="term" value="F:1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino]imidazole-4-carboxamide isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006062">
        <property type="entry name" value="His_biosynth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004650">
        <property type="entry name" value="HisA/F-archaeal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044524">
        <property type="entry name" value="Isoase_HisA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43090">
        <property type="entry name" value="PTHR43090"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00977">
        <property type="entry name" value="His_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="SSF51366"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00734">
        <property type="entry name" value="hisAF_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="2" id="KW-0368">Histidine biosynthesis</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF96475.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="222" mass="24825" checksum="8DDF7A32E67ACF34" modified="2011-07-27" version="1">MEIIPVLDIMRGLAVHGKSGKREEYKPLKSVLCNSSNPIEIAEKYKENGAETVYIADLDAIMEKGNNFEIIREIDMNKIVDAGVRDKDDLQNVLKICDKAIIGTETLRDLNLLKEKDIILSLDFKDGKLLNYDLDMILSYVDEKTPLIILDISSVGTQRGINVELIKDVINKVNNPIYIGGGIRDEEDVKIAYNLGVNGILIATAIHKGILNLRELIEKYGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDC6</accession>
    <name>F6BDC6_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">TATA-box-binding protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Box A-binding protein</fullName>
            <shortName evidence="6">BAP</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">TATA sequence-binding protein</fullName>
            <shortName evidence="6">TBP</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">TATA-box factor</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">tbp</name>
        <name evidence="8" type="ordered locus">Metig_0944</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5 6 7">General factor that plays a role in the activation of archaeal genes transcribed by RNA polymerase. Binds specifically to the TATA box promoter element which lies close to the position of transcription initiation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6 7">Belongs to the TBP family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96487.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799089.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0944"/>
    <dbReference type="EnsemblBacteria" id="AEF96487">
        <property type="protein sequence ID" value="AEF96487"/>
        <property type="gene ID" value="Metig_0944"/>
    </dbReference>
    <dbReference type="GeneID" id="10643790"/>
    <dbReference type="KEGG" id="mig:Metig_0944"/>
    <dbReference type="HOGENOM" id="CLU_060161_4_3_2"/>
    <dbReference type="OMA" id="NCEYEPE"/>
    <dbReference type="OrthoDB" id="83880at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription, initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04518">
        <property type="entry name" value="TBP_archaea"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00408">
        <property type="entry name" value="TATA_bind_prot_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000814">
        <property type="entry name" value="TBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033711">
        <property type="entry name" value="TBP_archaea"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030491">
        <property type="entry name" value="TBP_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012295">
        <property type="entry name" value="TBP_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10126">
        <property type="entry name" value="PTHR10126"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00352">
        <property type="entry name" value="TBP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00686">
        <property type="entry name" value="TIFACTORIID"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00351">
        <property type="entry name" value="TFIID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6 7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 6 7" id="KW-0804">Transcription</keyword>
    <keyword evidence="2 6 7" id="KW-0805">Transcription regulation</keyword>
    <feature type="repeat" description="1" evidence="6">
        <location>
            <begin position="7"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="repeat" description="2" evidence="6">
        <location>
            <begin position="98"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00025680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEF96487.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="181" mass="19961" checksum="5C52FF6E488AFAB5" modified="2011-07-27" version="1">MEPKIKIVNVVVSTQIGSDIDLEEAALILDNAEYEPEQFPGLVCRLSDPKVALLIFRSGKVNCTGAKSKEEAEVAIKKIIKELKDAGMDIIDNPEVKVQNMVATADLGIEPNLDDIALMIEGTEYEPEQFPGLVYRLDDPKVVVLIFGSGKVVITGLKREEDAEIALNKILETLREIEEII</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDE3</accession>
    <name>F6BDE3_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Phosphoesterase RecJ domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_0961</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96504.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799106.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0961"/>
    <dbReference type="EnsemblBacteria" id="AEF96504">
        <property type="protein sequence ID" value="AEF96504"/>
        <property type="gene ID" value="Metig_0961"/>
    </dbReference>
    <dbReference type="GeneID" id="10643807"/>
    <dbReference type="KEGG" id="mig:Metig_0961"/>
    <dbReference type="HOGENOM" id="CLU_070736_0_0_2"/>
    <dbReference type="OMA" id="CHHNADP"/>
    <dbReference type="OrthoDB" id="47681at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001667">
        <property type="entry name" value="DDH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038763">
        <property type="entry name" value="DHH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003156">
        <property type="entry name" value="DHHA1_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01368">
        <property type="entry name" value="DHH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02272">
        <property type="entry name" value="DHHA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64182">
        <property type="entry name" value="SSF64182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DHH" evidence="1">
        <location>
            <begin position="20"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="domain" description="DHHA1" evidence="2">
        <location>
            <begin position="220"/>
            <end position="311"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF96504.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="336" mass="37689" checksum="1D5A41C2F70A40FC" modified="2011-07-27" version="1">MEPLQENIENLLKFLKNDEILFLCHHNADPDAVGASIALKYLAKTLNPSGNFRISANSISKLSRNILEEVDEEVEIVEYPKLPKTVVLVDTSSTNQLSVDEDKLKECDVVLIDHHKKTNLVNLCKFYIVDEKSPSTCEIVSEIFRKLGIYPPKNIRIALLCGILYDTKHLKLANEMTFSILAWLIKGISFQKILYLLSQESDKSKRVAHLKACSRMEIREVDGITVALSHVSSHEASSAKTIVSIGADIAFVVAVRKREEEIRVSARCRKSVSKKVHLGSLMEKIGKELGGEGGGHAEAAGLNAPYDKKKKNKDDVIKEVLNLCFEMFKKEWENIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDF7</accession>
    <name>F6BDF7_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Class III signal peptide-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_0975</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96518.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799120.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0975"/>
    <dbReference type="EnsemblBacteria" id="AEF96518">
        <property type="protein sequence ID" value="AEF96518"/>
        <property type="gene ID" value="Metig_0975"/>
    </dbReference>
    <dbReference type="GeneID" id="10643821"/>
    <dbReference type="KEGG" id="mig:Metig_0975"/>
    <dbReference type="HOGENOM" id="CLU_143949_0_0_2"/>
    <dbReference type="OMA" id="YAGMTWD"/>
    <dbReference type="OrthoDB" id="110791at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007166">
        <property type="entry name" value="Class3_signal_pept_motif"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04021">
        <property type="entry name" value="Class_IIIsignal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF96518.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="125" mass="14226" checksum="4E99D7F940D9EC4B" modified="2011-07-27" version="1">MLFKRMAGQISLEFSLLVLVVLVVSMSLIVNIMNDLFNEEERIIDKIDIAAKTAVSLVNSHYNGTYANETLIYTGLTYNESNGSVYVTIHIIPKNAINKDVGDFIINYIYDNQHINRSKYNISIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-06-02" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDG2</accession>
    <name>F6BDG2_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_0980</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96523.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799125.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96523">
        <property type="protein sequence ID" value="AEF96523"/>
        <property type="gene ID" value="Metig_0980"/>
    </dbReference>
    <dbReference type="GeneID" id="10643827"/>
    <dbReference type="KEGG" id="mig:Metig_0980"/>
    <dbReference type="HOGENOM" id="CLU_878828_0_0_2"/>
    <dbReference type="OMA" id="NFCILNN"/>
    <dbReference type="OrthoDB" id="46385at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF96523.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="310" mass="36259" checksum="31B1A48C46E7C5B6" modified="2011-07-27" version="1">MERIYSKYGNISTLKEVISFLDNFTGIIKIDNAKLFYKNSKLILSTYNDREKDLEEIIKKLPKFFLIEIYRTSSNEIEELISHNKLIEKDHSVINFVEVNKLDINYGNGILINAYKNIFNYINGLCKVEFRFKKYKGDEGTIVYKDNEEILAIYKQKDKILEGKRALNKIKTIFAVSDVFAFIDKISEEELNKIIDKHPNAVLKVVVSFDELIKNIKSKEYKVVEDDSLLEVLTEEPSLIEIVGENAYIVSKNKNAMYAFLNDYDGDKAFRIIKNICILNKITFKIYPLTEKEFEMFKEFKKNRVKVSLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDG8</accession>
    <name>F6BDG8_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 7">Ribosome biogenesis protein Nop10</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">nop10</name>
        <name evidence="8" type="ordered locus">Metig_0987</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 7">Involved in ribosome biogenesis; more specifically in 18S rRNA pseudouridylation and in cleavage of pre-rRNA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the NOP10 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96529.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0987"/>
    <dbReference type="EnsemblBacteria" id="AEF96529">
        <property type="protein sequence ID" value="AEF96529"/>
        <property type="gene ID" value="Metig_0987"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0987"/>
    <dbReference type="HOGENOM" id="CLU_196480_1_0_2"/>
    <dbReference type="OMA" id="FRIRKCP"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030515">
        <property type="term" value="F:snoRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001522">
        <property type="term" value="P:pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00803">
        <property type="entry name" value="Nop10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007264">
        <property type="entry name" value="H/ACA_rnp_Nop10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036756">
        <property type="entry name" value="H/ACA_rnp_Nop10_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023532">
        <property type="entry name" value="Nop10_arc-typ"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04135">
        <property type="entry name" value="Nop10p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144210">
        <property type="entry name" value="SSF144210"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 7" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="5 7" id="KW-0698">rRNA processing</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEF96529.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="52" mass="6183" checksum="2A9A203726040CA3" modified="2011-07-27" version="1">MKKCLKCGRYTLKELCPNCGEKTMVPKPPKFSLEDRYGKYRRMLKKSLKNKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDH7</accession>
    <name>F6BDH7_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Roadblock/LC7 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0996</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96538.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799140.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0996"/>
    <dbReference type="EnsemblBacteria" id="AEF96538">
        <property type="protein sequence ID" value="AEF96538"/>
        <property type="gene ID" value="Metig_0996"/>
    </dbReference>
    <dbReference type="GeneID" id="10643843"/>
    <dbReference type="KEGG" id="mig:Metig_0996"/>
    <dbReference type="HOGENOM" id="CLU_118613_4_0_2"/>
    <dbReference type="OMA" id="NVGRIRY"/>
    <dbReference type="OrthoDB" id="103616at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060090">
        <property type="term" value="F:molecular adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032008">
        <property type="term" value="P:positive regulation of TOR signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037587">
        <property type="entry name" value="LAMTOR2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004942">
        <property type="entry name" value="Roadblock/LAMTOR2_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13323">
        <property type="entry name" value="PTHR13323"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03259">
        <property type="entry name" value="Robl_LC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00960">
        <property type="entry name" value="Robl_LC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Robl_LC7" evidence="1">
        <location>
            <begin position="2"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96538.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="114" mass="11948" checksum="865625B47D511A97" modified="2011-07-27" version="1">MIDRILADLNRTEGIKGSMVVGKDGLVIAAQLPSNIDSELVGAMASAAFGAAERTAAEIGQGKLEQAMIESEFGKTLMTDAGEGILVVLTDAKVNLGLIRISMKRAAEKIKNAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDI4</accession>
    <name>F6BDI4_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Intracellular protease, PfpI family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_1003</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C56 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96545.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799147.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1003"/>
    <dbReference type="EnsemblBacteria" id="AEF96545">
        <property type="protein sequence ID" value="AEF96545"/>
        <property type="gene ID" value="Metig_1003"/>
    </dbReference>
    <dbReference type="GeneID" id="10643851"/>
    <dbReference type="KEGG" id="mig:Metig_1003"/>
    <dbReference type="HOGENOM" id="CLU_000445_44_4_2"/>
    <dbReference type="OMA" id="IGKYGVP"/>
    <dbReference type="OrthoDB" id="98884at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006286">
        <property type="entry name" value="C56_PfpI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002818">
        <property type="entry name" value="DJ-1/PfpI"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01965">
        <property type="entry name" value="DJ-1_PfpI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="SSF52317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01382">
        <property type="entry name" value="PfpI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DJ-1_PfpI" evidence="2">
        <location>
            <begin position="33"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF96545.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="202" mass="22195" checksum="A5E0038AB241355D" modified="2011-07-27" version="1">MRGLIFLSILLLATICGCVEKKITNETEDDTVSVLIVIAPKDFRDEELFEPLQIFKENGFNVEVVSTTRGEHVGMLGGKITTTKTIDEVNAKDYDAIVIVGGIGSKEYLWDNVELQELVKEFYNDGKVVSAICLSPVVLARAGILKDKEATVYAAKEAIKELEKHGATYVDKGVVVDGNIVTAKNPNYAKDFGLEIVKLLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDJ2</accession>
    <name>F6BDJ2_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 13">Inosine-5'-monophosphate dehydrogenase</fullName>
            <shortName evidence="6">IMP dehydrogenase</shortName>
            <shortName evidence="6">IMPD</shortName>
            <shortName evidence="6">IMPDH</shortName>
            <ecNumber evidence="6 13">1.1.1.205</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">guaB</name>
        <name evidence="15" type="ordered locus">Metig_1011</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes the conversion of inosine 5'-phosphate (IMP) to xanthosine 5'-phosphate (XMP), the first committed and rate-limiting step in the de novo synthesis of guanine nucleotides, and therefore plays an important role in the regulation of cell growth.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 13">
            <text>H2O + IMP + NAD(+) = H(+) + NADH + XMP</text>
            <dbReference type="Rhea" id="RHEA:11708"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57464"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <dbReference type="EC" id="1.1.1.205"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </cofactor>
    </comment>
    <comment type="activity regulation">
        <text evidence="6">Mycophenolic acid (MPA) is a non-competitive inhibitor that prevents formation of the closed enzyme conformation by binding to the same site as the amobile flap. In contrast, mizoribine monophosphate (MZP) is a competitive inhibitor that induces the closed conformation. MPA is a potent inhibitor of mammalian IMPDHs but a poor inhibitor of the bacterial enzymes. MZP is a more potent inhibitor of bacterial IMPDH.</text>
    </comment>
    <comment type="pathway">
        <text evidence="6 13">Purine metabolism; XMP biosynthesis via de novo pathway; XMP from IMP: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6 12">Belongs to the IMPDH/GMPR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.205" evidence="6 13"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96553.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799155.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1011"/>
    <dbReference type="EnsemblBacteria" id="AEF96553">
        <property type="protein sequence ID" value="AEF96553"/>
        <property type="gene ID" value="Metig_1011"/>
    </dbReference>
    <dbReference type="GeneID" id="10643865"/>
    <dbReference type="KEGG" id="mig:Metig_1011"/>
    <dbReference type="HOGENOM" id="CLU_022552_2_1_2"/>
    <dbReference type="OMA" id="MGYCGAK"/>
    <dbReference type="OrthoDB" id="58700at2157"/>
    <dbReference type="UniPathway" id="UPA00601">
        <property type="reaction ID" value="UER00295"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003938">
        <property type="term" value="F:IMP dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006177">
        <property type="term" value="P:GMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00381">
        <property type="entry name" value="IMPDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01964">
        <property type="entry name" value="IMPDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005990">
        <property type="entry name" value="IMP_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015875">
        <property type="entry name" value="IMP_DH/GMP_Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001093">
        <property type="entry name" value="IMP_DH_GMPRt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11911">
        <property type="entry name" value="PTHR11911"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00478">
        <property type="entry name" value="IMPDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000130">
        <property type="entry name" value="IMPDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="SSF54631"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01302">
        <property type="entry name" value="IMP_dehydrog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00487">
        <property type="entry name" value="IMP_DH_GMP_RED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 11" id="KW-0129">CBS domain</keyword>
    <keyword evidence="6 13" id="KW-0332">GMP biosynthesis</keyword>
    <keyword evidence="2 6 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 9 13" id="KW-0520">NAD</keyword>
    <keyword evidence="4 6 12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3 6 10" id="KW-0630">Potassium</keyword>
    <keyword evidence="6 13" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBS" evidence="14">
        <location>
            <begin position="96"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="14">
        <location>
            <begin position="156"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="region of interest" description="IMP binding" evidence="6 8">
        <location>
            <begin position="339"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="region of interest" description="IMP binding" evidence="6 8">
        <location>
            <begin position="362"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="region of interest" description="IMP binding" evidence="6 8">
        <location>
            <begin position="386"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="active site" description="Thioimidate intermediate" evidence="6 7">
        <location>
            <position position="306"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="6 7">
        <location>
            <position position="405"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="247"/>
            <end position="249"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="247"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6 9">
        <location>
            <begin position="299"/>
            <end position="301"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6 10">
        <location>
            <position position="301"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6 10">
        <location>
            <position position="303"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6 8">
        <location>
            <position position="304"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6 10">
        <location>
            <position position="306"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6 8">
        <location>
            <position position="419"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="473"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
            <note>ligand shared between two tetrameric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="474"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
            <note>ligand shared between two tetrameric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="475"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
            <note>ligand shared between two tetrameric partners</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000130-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000130-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000130-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000130-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU003928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="AEF96553.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="492" mass="52968" checksum="9FD6BD2096458EB7" modified="2011-07-27" version="1">MFLDKIVNAKMAYTFDDVLLVPNASYVEPKDTDVSTDLAGLKLNIPILSAAMDTVTEKEMAIALARRGGLGVIHRNMSIQDQVKHVHAVKMADEYIVKDVITISPDCTVSEAVRIMDENSVSGLPVVDESDKLVGIITLRDIKPIKDRSIKVKEVMTKDVVSVTEDITHDEALNVMYENRIERLPIVDENNKLVGMITLRDILKRKQYPEAARDKKGRLLVAAACGPYDLERARALVEAEVDAIAIDCAHAHNMKVVEHVKILKKELEGTDVKLIVGNIATKEAAEDLIAAGADVLKVGIGPGSICTTRVVAGVGVPQLTAVAEVADVAKEHNVPVIADGGIRYSGDIAKAIAAGADAVMLGSLLAGTDEAPGQLMVINGRKYKQYRGMGSLGAMTGGVGAGADRYFQSHMKHVKLVPEGVEGAVPYKGPVSEVIFQLIGGLRASMGYCGARTIKEMQEKARFVLITQSGQRESHPHDIIITNEAPNYPLGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDL5</accession>
    <name>F6BDL5_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PyrE-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metig_1034</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the purine/pyrimidine phosphoribosyltransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96576.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799178.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1034"/>
    <dbReference type="EnsemblBacteria" id="AEF96576">
        <property type="protein sequence ID" value="AEF96576"/>
        <property type="gene ID" value="Metig_1034"/>
    </dbReference>
    <dbReference type="GeneID" id="10643888"/>
    <dbReference type="KEGG" id="mig:Metig_1034"/>
    <dbReference type="HOGENOM" id="CLU_111001_0_0_2"/>
    <dbReference type="OMA" id="DIHVDWS"/>
    <dbReference type="OrthoDB" id="77963at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01214">
        <property type="entry name" value="PyrE_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRibTrfase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022854">
        <property type="entry name" value="PyrE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013324">
        <property type="entry name" value="RNA_pol_sigma_r3/r4-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19278:SF41">
        <property type="entry name" value="PTHR19278:SF41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00156">
        <property type="entry name" value="Pribosyltran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="SSF53271"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88659">
        <property type="entry name" value="SSF88659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Pribosyltran" evidence="3">
        <location>
            <begin position="69"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEF96576.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="209" mass="23551" checksum="7709C1845901334A" modified="2011-07-27" version="1">MKKELIKKVIKLKDKGLTIGEIADELNVSMDTALYLVLNAEKLLKEEEVKETTEKRKKLDIFVEWNTVGNSSKRLRYIASIMCDILKNVEFDAVVGIATSGVPLATMISDEMDKELSIYIPKKHVHDEGKKITGIISHNFSNIEHKNIVIIDDVMTTGSTIKEAIKYLREIANPKMVVVMIDKSGINEIEGIPVVPLFRVGIVEIEDRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDR6</accession>
    <name>F6BDR6_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="3">6-phospho-3-hexuloisomerase</fullName>
            <ecNumber evidence="3">5.3.1.27</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_1088</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SIS family. PHI subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.27" evidence="3"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96627.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1088"/>
    <dbReference type="EnsemblBacteria" id="AEF96627">
        <property type="protein sequence ID" value="AEF96627"/>
        <property type="gene ID" value="Metig_1088"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_1088"/>
    <dbReference type="HOGENOM" id="CLU_094236_1_1_2"/>
    <dbReference type="OMA" id="KTIQPMG"/>
    <dbReference type="OrthoDB" id="106225at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097367">
        <property type="term" value="F:carbohydrate derivative binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043800">
        <property type="term" value="F:hexulose-6-phosphate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901135">
        <property type="term" value="P:carbohydrate derivative metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05005">
        <property type="entry name" value="SIS_PHI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017552">
        <property type="entry name" value="PHI/rmpB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001347">
        <property type="entry name" value="SIS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046348">
        <property type="entry name" value="SIS_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43443">
        <property type="entry name" value="PTHR43443"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01380">
        <property type="entry name" value="SIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53697">
        <property type="entry name" value="SSF53697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51464">
        <property type="entry name" value="SIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SIS" evidence="2">
        <location>
            <begin position="32"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF96627.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="176" mass="19577" checksum="D074679C584AECE8" modified="2011-07-27" version="1">MIGEILPRIIKNIELLIEFERDENLKNKLNSFLDAIINAKNIFVFGVGRSGLVGKAFAMRLMHLGFNVYVVGETICPSFKKGDLLIAISGSGETTLTIEIAKKADNVVAITCNVPNTLAKISNIWIPICISKNEYLPLGTAFEHIAFIFLDIVVALLMERLGISEKEMKERHCNLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDZ1</accession>
    <name>F6BDZ1_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Capsular polysaccharide biosynthesis protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1163</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96702.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799303.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96702">
        <property type="protein sequence ID" value="AEF96702"/>
        <property type="gene ID" value="Metig_1163"/>
    </dbReference>
    <dbReference type="GeneID" id="10644023"/>
    <dbReference type="KEGG" id="mig:Metig_1163"/>
    <dbReference type="HOGENOM" id="CLU_1154355_0_0_2"/>
    <dbReference type="OMA" id="QSYAKKE"/>
    <dbReference type="OrthoDB" id="42452at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025669">
        <property type="entry name" value="AAA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13614">
        <property type="entry name" value="AAA_31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA_31" evidence="1">
        <location>
            <begin position="1"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96702.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="258" mass="28785" checksum="2D6A2BCEA59649A4" modified="2011-07-27" version="1">MKTITFSIAKGGTGKSVMTANVAAALALKGKKVVILDADIGSRSLTHLFGKEMNKSFIDAIKEDLSIDEILVETDIENLYIITSGRSLEDYFKIEGYKAFEKLEELKRFDYVFIDAPTTTTGIETYLSLGFSEYFVLVLDFISFGPSLQGAINTAVIGRNYLECKPVGFILNKSDALVPESVINDVEKLLGMKCIANIPKNALLEQSYNRKEILYVKNTDANFNKLIDNIVEELEALPEYEKTSIPELLKRIKDSLIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>F6BE08</accession>
    <name>F6BE08_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Phosphate transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Metig_1181</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Potential transporter for phosphate.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96719.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1181"/>
    <dbReference type="EnsemblBacteria" id="AEF96719">
        <property type="protein sequence ID" value="AEF96719"/>
        <property type="gene ID" value="Metig_1181"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_1181"/>
    <dbReference type="HOGENOM" id="CLU_015355_0_0_2"/>
    <dbReference type="OMA" id="RMFVAWG"/>
    <dbReference type="OrthoDB" id="40216at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005315">
        <property type="term" value="F:inorganic phosphate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006817">
        <property type="term" value="P:phosphate ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001204">
        <property type="entry name" value="Phos_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11101">
        <property type="entry name" value="PTHR11101"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01384">
        <property type="entry name" value="PHO4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="26"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="67"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="92"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="148"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="178"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="211"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="241"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="269"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="300"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEF96719.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="321" mass="34519" checksum="AFBBC0BCEB0CEB0B" modified="2011-07-27" version="1">MEYLYINSSISNLLRSKERNFLDNKIMVIKIIEILIGLYIAFVIGANDAANALGTTVGAKLMSYKRAVLLFGIFVLIGCLNAHNVGHTMSSIVSGDVTLPLIIAGIIITFLTFKKIPISTHQVIVSALVGLNLNSADLNLFVKIVGSWIVSPILAGILAYIIYKIFEKLEIPILKRENFLKYGLIFSGGLIAYNLGANDLPTVLGTVSSELSIFVVGAIALWMGALFFGKGVSETVGMKLVELNPLPAFIAQLSAGLAVFVFTQFGMPVSTTQAIIGGVVGVGLTKGIKTVSWRTLRNVVLGWISAPTFALVIGYLIQPHF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>F6BE18</accession>
    <name>F6BE18_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Formate dehydrogenase</fullName>
            <ecNumber evidence="4">1.2.1.2</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metig_1191</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.2.1.2" evidence="4"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96729.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799328.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1191"/>
    <dbReference type="EnsemblBacteria" id="AEF96729">
        <property type="protein sequence ID" value="AEF96729"/>
        <property type="gene ID" value="Metig_1191"/>
    </dbReference>
    <dbReference type="GeneID" id="10644051"/>
    <dbReference type="KEGG" id="mig:Metig_1191"/>
    <dbReference type="HOGENOM" id="CLU_063409_0_0_2"/>
    <dbReference type="OMA" id="CAPTMFG"/>
    <dbReference type="OrthoDB" id="34322at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007516">
        <property type="entry name" value="Co_F420_Hydgase/DH_bsu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045220">
        <property type="entry name" value="FRHB/FDHB/HCAR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007525">
        <property type="entry name" value="FrhB_FdhB_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31332">
        <property type="entry name" value="PTHR31332"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04432">
        <property type="entry name" value="FrhB_FdhB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04422">
        <property type="entry name" value="FrhB_FdhB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0408">Iron</keyword>
    <keyword evidence="2" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="3">
        <location>
            <begin position="272"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="3">
        <location>
            <begin position="322"/>
            <end position="352"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEF96729.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="379" mass="43160" checksum="F81CB436623AD7EC" modified="2011-07-27" version="1">MDKYVLVQATDEEILKKAECGGAVTALFKYLLDKKLVDGILALKKGEDIYDGIPAFITDSEELIETAGSLHCAPTNFGKLVKNYLADKRIAVSVKPCDAMAIRELAKLNQINLDNVYMIGLNCGGTISPITAMRMIELFYKVDPKDVVKEEIDKGKFIIKLKNGEHKAVKIHELEVKSFGRRRNCQRCELKIPRNADLACGNWGAEPGWTFVEICSEKGRELIENAEKEGYIKTKKPSEKGIEIRAKIENSMIKLAKNFQKKHLEENYPSIKEWQKYWNRCIKCYGCRDACPICFCIECKLEKDYVDEKGKIPPNPLMFQGIRLSHVSQSCINCGQCEDVCPMDIPLAYIYHRMQLKFRDTFGYIPGVDDKTPPLFDFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>F6BE24</accession>
    <name>F6BE24_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Hydrogen dehydrogenase</fullName>
            <ecNumber evidence="3">1.12.1.2</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_1197</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit family.</text>
    </comment>
    <dbReference type="EC" id="1.12.1.2" evidence="3"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96735.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799334.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1197"/>
    <dbReference type="EnsemblBacteria" id="AEF96735">
        <property type="protein sequence ID" value="AEF96735"/>
        <property type="gene ID" value="Metig_1197"/>
    </dbReference>
    <dbReference type="GeneID" id="10644057"/>
    <dbReference type="KEGG" id="mig:Metig_1197"/>
    <dbReference type="HOGENOM" id="CLU_044556_0_0_2"/>
    <dbReference type="OMA" id="VQNNPAM"/>
    <dbReference type="OrthoDB" id="32760at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008901">
        <property type="term" value="F:ferredoxin hydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047985">
        <property type="term" value="F:hydrogen dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016151">
        <property type="term" value="F:nickel cation binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.645.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001501">
        <property type="entry name" value="Ni-dep_hyd_lsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018194">
        <property type="entry name" value="Ni-dep_hyd_lsu_Ni_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029014">
        <property type="entry name" value="NiFe-Hase_large"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00374">
        <property type="entry name" value="NiFeSe_Hases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56762">
        <property type="entry name" value="SSF56762"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00507">
        <property type="entry name" value="NI_HGENASE_L_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00508">
        <property type="entry name" value="NI_HGENASE_L_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0533">Nickel</keyword>
    <keyword evidence="1 2 3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF96735.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="473" mass="53162" checksum="7AF54A2D10CA2BE2" modified="2011-07-27" version="1">MVKLSIEPVTRVEGHGKITVSFDENGNLDKVHFHVVEVRGFEKFLEGRYIEDAPIYTPRICGICQVAHHLASAKAVDAVFGVKIPETANLLRNLMHQGSTIHSHALHFYMLAAPDLMFPTTDDVLKRNLIGIAKEHPEIVKDAIELRKIGQTIVKVVGGRAIHPVTCVVGGQSKPLKEEEREELLKLSERAIELSEKSIEIGKKLIENIKEKDLLDIGYFESYHMGMVNDGVHELYDGKIRVVNAEGKVEYEFDPSEYMNYIAEGVRPYSYLKFPYLKDKGEEGGVYRVNTLSRLNVADKMATPLAQKYYEEFVKEFGKPCHYPMLFHYARLIELLSSAEKVKELLENDKIVGEDIRAEPEDIVGEGVGCVEAPRGTLIHHFKTNEEGIITDANLIVATVQNNPAMDIGVRKVAEKYLKSPDDANPQVLNYMEMVIRAYDPCLSCATHVIGNSENGLSLEVYKKNELVKVIRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>F6BE40</accession>
    <name>F6BE40_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="3">CBS domain containing membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_0014</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95576.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798186.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0014"/>
    <dbReference type="EnsemblBacteria" id="AEF95576">
        <property type="protein sequence ID" value="AEF95576"/>
        <property type="gene ID" value="Metig_0014"/>
    </dbReference>
    <dbReference type="GeneID" id="10642849"/>
    <dbReference type="KEGG" id="mig:Metig_0014"/>
    <dbReference type="HOGENOM" id="CLU_076812_0_0_2"/>
    <dbReference type="OMA" id="GSDWAFN"/>
    <dbReference type="OrthoDB" id="67420at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="SSF54631"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0129">CBS domain</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBS" evidence="2">
        <location>
            <begin position="6"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="2">
        <location>
            <begin position="88"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="2">
        <location>
            <begin position="152"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="2">
        <location>
            <begin position="227"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF95576.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="278" mass="31351" checksum="E65E5AFA4F6F6E59" modified="2011-07-27" version="1">MKINKIINGKEIVTVYPTTTIRKTLVTMNEEGFRRIPVVDAGTNRVEGIVTSMDIVDFMGGGSKYNLIREKHNRNFIAAINEPVREIMEEDVITIKENAEIDDIIKTFLEKNVGGTPIVNNNNQLISLITERDVIRVLKDKISEEEKVMDYMTKKVIAATAGERLKDVARTMLRNGFRRLPVVSEGKLVGIITSTDFIKLLGSDWAFNHMQTGNVREITNVRMQDIMIKDVITAREEHSLKDVAEIMTSKNIGALPVVEDGELVGIITEKDIIKYFSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>F6BE43</accession>
    <name>F6BE43_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="5">L-aspartate oxidase</fullName>
            <ecNumber evidence="5">1.4.3.16</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Metig_0017</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.4.3.16" evidence="5"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95579.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798189.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0017"/>
    <dbReference type="EnsemblBacteria" id="AEF95579">
        <property type="protein sequence ID" value="AEF95579"/>
        <property type="gene ID" value="Metig_0017"/>
    </dbReference>
    <dbReference type="GeneID" id="10642852"/>
    <dbReference type="KEGG" id="mig:Metig_0017"/>
    <dbReference type="HOGENOM" id="CLU_014312_8_1_2"/>
    <dbReference type="OMA" id="PTAHHFM"/>
    <dbReference type="OrthoDB" id="8547at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008734">
        <property type="term" value="F:L-aspartate oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044318">
        <property type="term" value="F:L-aspartate:fumarate oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.700.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003953">
        <property type="entry name" value="FAD-binding_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037099">
        <property type="entry name" value="Fum_R/Succ_DH_flav-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015939">
        <property type="entry name" value="Fum_Rdtase/Succ_DH_flav-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030664">
        <property type="entry name" value="SdhA/FrdA/AprA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027477">
        <property type="entry name" value="Succ_DH/fumarate_Rdtase_cat_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11632">
        <property type="entry name" value="PTHR11632"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00890">
        <property type="entry name" value="FAD_binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02910">
        <property type="entry name" value="Succ_DH_flav_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46977">
        <property type="entry name" value="SSF46977"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56425">
        <property type="entry name" value="SSF56425"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="2 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD_binding_2" evidence="3">
        <location>
            <begin position="4"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="domain" description="Succ_DH_flav_C" evidence="4">
        <location>
            <begin position="432"/>
            <end position="519"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEF95579.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="536" mass="59482" checksum="E77E29DDA07DD93D" modified="2011-07-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>F6BE46</accession>
    <name>F6BE46_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Phosphate uptake regulator, PhoU</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0020</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95582.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798192.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0020"/>
    <dbReference type="EnsemblBacteria" id="AEF95582">
        <property type="protein sequence ID" value="AEF95582"/>
        <property type="gene ID" value="Metig_0020"/>
    </dbReference>
    <dbReference type="GeneID" id="10642855"/>
    <dbReference type="KEGG" id="mig:Metig_0020"/>
    <dbReference type="HOGENOM" id="CLU_989064_0_0_2"/>
    <dbReference type="OMA" id="DRCVNIV"/>
    <dbReference type="OrthoDB" id="51309at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030643">
        <property type="term" value="P:cellular phosphate ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045936">
        <property type="term" value="P:negative regulation of phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.220">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028366">
        <property type="entry name" value="P_transport_PhoU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038078">
        <property type="entry name" value="PhoU-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026022">
        <property type="entry name" value="PhoU_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42930">
        <property type="entry name" value="PTHR42930"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01895">
        <property type="entry name" value="PhoU"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PhoU" evidence="1">
        <location>
            <begin position="83"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="domain" description="PhoU" evidence="1">
        <location>
            <begin position="183"/>
            <end position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF95582.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="281" mass="32661" checksum="2E447D770302CD48" modified="2011-07-27" version="1">MLRGKDATLAAILKVIMEDKPETQDEIANILGVSRRYVAKLLKPLIDEGVVRHPYVVDLEKLSKLNIKFDTDIILKEILNTFERMGNNVLLNLDKAFMALKNNDIETAKNIIIQDYTLNRMEEEVNIMIKMDALRYLPTTYAMNLSSIASNIERCGDYISNISEEVVNGLKIKNKELQREIDEIYLIIREMFVEAMDILKTKKRDSKIFELEDKLHEKLEKIMEKVSKEKGENINYYIQFGMFLKDVERFGDRCVNIVEIARELYHGIPRSVLPEGLKKNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F6BE47</accession>
    <name>F6BE47_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Metig_0021</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0014 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95583.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798193.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0021"/>
    <dbReference type="EnsemblBacteria" id="AEF95583">
        <property type="protein sequence ID" value="AEF95583"/>
        <property type="gene ID" value="Metig_0021"/>
    </dbReference>
    <dbReference type="GeneID" id="10642856"/>
    <dbReference type="KEGG" id="mig:Metig_0021"/>
    <dbReference type="HOGENOM" id="CLU_076147_1_0_2"/>
    <dbReference type="OMA" id="PWYEPQY"/>
    <dbReference type="OrthoDB" id="70864at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005226">
        <property type="entry name" value="UPF0014_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30028">
        <property type="entry name" value="PTHR30028"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03649">
        <property type="entry name" value="UPF0014"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00245">
        <property type="entry name" value="TIGR00245"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="35"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="60"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="89"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="120"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="189"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="219"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEF95583.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="246" mass="27631" checksum="203CE8965E633278" modified="2011-07-27" version="1">MISFLSTIVPAFIFVIMSIIIAYKEELGLEKKILIVCISALIQLLILGFIINIIFNLGLFFTFLMIFVMILIASYMIKNEININERSIIFLSSCFSLLFTSTISLLILIYSGIVELKPQYIIPLVGMVIGNSMNSITLCLERLLNDLKSNKDILWGYLALGANDYQAMKPFIRNAIKAALIPQMNRTKTVGLIFIPGAMVGMLLSGADPIRAAEIQIVIMWMILSGTLLSTLITCYLIYKKFMKYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>F6BE86</accession>
    <name>F6BE86_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Xylose isomerase domain-containing protein TIM barrel</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1226</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96763.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799362.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1226"/>
    <dbReference type="EnsemblBacteria" id="AEF96763">
        <property type="protein sequence ID" value="AEF96763"/>
        <property type="gene ID" value="Metig_1226"/>
    </dbReference>
    <dbReference type="GeneID" id="10644087"/>
    <dbReference type="KEGG" id="mig:Metig_1226"/>
    <dbReference type="HOGENOM" id="CLU_050006_7_2_2"/>
    <dbReference type="OMA" id="HIHDNMG"/>
    <dbReference type="OrthoDB" id="46548at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001719">
        <property type="entry name" value="AP_endonuc_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036237">
        <property type="entry name" value="Xyl_isomerase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013022">
        <property type="entry name" value="Xyl_isomerase-like_TIM-brl"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01261">
        <property type="entry name" value="AP_endonuc_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00518">
        <property type="entry name" value="AP2Ec"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51658">
        <property type="entry name" value="SSF51658"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0413">Isomerase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AP_endonuc_2" evidence="1">
        <location>
            <begin position="20"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96763.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="276" mass="31025" checksum="0A51D63CAF7C0194" modified="2011-07-27" version="1">MKFGISSLVFLPEGLKSTMEKIAENHFDCWEIVCEGNHYLSPKNIKYLMELKDRYEVDIVVHAPFSDLNPASMNERVRRLTVDCITDAIEGAFELDASVVVVHPGYIPPLWSNYVEDILDNNFSTLSDIVEVAEDYGITIGLENMPNFKGVLGITPESLREIVKDIDSKYLGITFDIGHANTIGNPADFVEELNSIGEGIVHVHIHDNNGYDDEHLKIGEGKIDFLSVLKKLKEIKYDGVLSIENKNVRDAVKSKEVLNEYLSILDDLKKGIEVEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F6BE92</accession>
    <name>F6BE92_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Methanogenesis marker protein 10</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Metig_1232</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96769.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1232"/>
    <dbReference type="EnsemblBacteria" id="AEF96769">
        <property type="protein sequence ID" value="AEF96769"/>
        <property type="gene ID" value="Metig_1232"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_1232"/>
    <dbReference type="HOGENOM" id="CLU_668380_0_0_2"/>
    <dbReference type="OMA" id="IHLGYTS"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaB/NifB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017672">
        <property type="entry name" value="MA_4551-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03278">
        <property type="entry name" value="methan_mark_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Elp3" evidence="5">
        <location>
            <begin position="24"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEF96769.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="428" mass="48384" checksum="71F2C6199915FAA1" modified="2011-07-27" version="1">MNFGMNLRMDFEDNKKNKKAQLLVDLKGEPGKDCNGFCRFCYFRKANSKDVFPFGCKHCTFQIGCDYCTYSVREMNGNFLPLPFVLQQVQGALFFNKYDKVNITGGGDISCYPFLEELCKYISDMGLKIHLGYTSGKGFGSLDIAKNLVDYGVDEVTFSVFSTNEKLRKEWMNDKNAKTSLECLKYFCENCEVHCAIIVVPGVNDGKELIKTVGDLVDWGAKAVILMRFANKIEQGLILENAPIVEGIKPHTIEEFKSIVKEIHETFGNKVRVSGTPLYDPVTNTPFAIAHEENILNKLKNKIECEATIITGNVAYPFLKKIFDETPVNVVKVNKDIADLITAKDLEQINLKELSDTVFIPPRAFVHDRVAEEILTRDGEKRMILRGIEQLTLDGEVSGIYTKKEAIEFELNAFNELIGMINFFGVRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEB4</accession>
    <name>F6BEB4_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_0720</name>
        <name evidence="4" type="ordered locus">Metig_1254</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96270.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96791.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798873.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96270">
        <property type="protein sequence ID" value="AEF96270"/>
        <property type="gene ID" value="Metig_0720"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96791">
        <property type="protein sequence ID" value="AEF96791"/>
        <property type="gene ID" value="Metig_1254"/>
    </dbReference>
    <dbReference type="GeneID" id="10644117"/>
    <dbReference type="KEGG" id="mig:Metig_0720"/>
    <dbReference type="KEGG" id="mig:Metig_1254"/>
    <dbReference type="HOGENOM" id="CLU_086561_0_0_2"/>
    <dbReference type="OMA" id="YEFLTPW"/>
    <dbReference type="OrthoDB" id="85074at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020209">
        <property type="entry name" value="Cas6b_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041528">
        <property type="entry name" value="Cas6b_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17262">
        <property type="entry name" value="Cas6b_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17955">
        <property type="entry name" value="Cas6b_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cas6b_N" evidence="2">
        <location>
            <begin position="1"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="domain" description="Cas6b_C" evidence="1">
        <location>
            <begin position="109"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF17262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF17955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF96270.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEF96791.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="221" mass="25593" checksum="2AAC9C9E9525BD5D" modified="2011-07-27" version="1">MELPILMCRLKTNEPLKKSQTPYLRGYILNEFNKEDYKELHNHSNNGFIYTYPKIQYKIIGGDAVLIGIKEGINILGEIVLDIKKLELNHKVYTVTGGYVKVVFEEFGVSDEMKRYKFISPWVALNEKNYLKYKELDEDGRKNLLEKILIGNVLSMSKYLDYTVEEKLIVEILDVESLIVRYKGNKFIGFKGEFEINFDIPNYLGIGRKVSKGFGSVVKVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F6BED4</accession>
    <name>F6BED4_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Xylose isomerase domain-containing protein TIM barrel</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1274</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96811.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1274"/>
    <dbReference type="EnsemblBacteria" id="AEF96811">
        <property type="protein sequence ID" value="AEF96811"/>
        <property type="gene ID" value="Metig_1274"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_1274"/>
    <dbReference type="HOGENOM" id="CLU_1264580_0_0_2"/>
    <dbReference type="OMA" id="TIHLHNG"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036237">
        <property type="entry name" value="Xyl_isomerase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013022">
        <property type="entry name" value="Xyl_isomerase-like_TIM-brl"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01261">
        <property type="entry name" value="AP_endonuc_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51658">
        <property type="entry name" value="SSF51658"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0413">Isomerase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AP_endonuc_2" evidence="1">
        <location>
            <begin position="102"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96811.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="241" mass="27841" checksum="68CC362CA18A8ACB" modified="2011-07-27" version="1">MICLYILIAYIYDIMTYKGGCLLGVCVRIKNTNLIEKFKQFELIDWGLHFCPYNKKMENIVGFHAPIVDLGNSNSNALSILKHTVDMVSDHDYLTLHITNGKDPNIDVLISNLSELVDYARKRNVKICIENLRTGFSSNPNNLIEIADATGCLITYDIGHVDYENRVKYIDIFSDKIYNVHVYELEVPKIGHIAPKNLENLRPTLDKLLDVGCDFWLIELMDIEEIIRTKKLCEEYLKEHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-06-02" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEE7</accession>
    <name>F6BEE7_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_0047</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95608.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798217.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF95608">
        <property type="protein sequence ID" value="AEF95608"/>
        <property type="gene ID" value="Metig_0047"/>
    </dbReference>
    <dbReference type="GeneID" id="10642882"/>
    <dbReference type="KEGG" id="mig:Metig_0047"/>
    <dbReference type="HOGENOM" id="CLU_2353229_0_0_2"/>
    <dbReference type="OMA" id="CYYRSGF"/>
    <dbReference type="OrthoDB" id="123451at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF95608.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="96" mass="11210" checksum="96575C510BA7C2FD" modified="2011-07-27" version="1">MKLCVDGVFYSREPLNFKKILEGIVEILGEDVKVLSLEFPEIAAIVGDTYYYRCGFMLDKELEEEIPEDELKKIKEKIKKLFPEDTIIYTLNCEIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEG0</accession>
    <name>F6BEG0_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Histidine--tRNA ligase</fullName>
            <ecNumber evidence="8">6.1.1.21</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Histidyl-tRNA synthetase</fullName>
            <shortName evidence="8">HisRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">hisS</name>
        <name evidence="10" type="ordered locus">Metig_0060</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>ATP + L-histidine + tRNA(His) = AMP + diphosphate + H(+) + L-histidyl-tRNA(His)</text>
            <dbReference type="Rhea" id="RHEA:17313"/>
            <dbReference type="Rhea" id="RHEA-COMP:9665"/>
            <dbReference type="Rhea" id="RHEA-COMP:9689"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57595"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78527"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.21"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.21" evidence="8"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95621.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798230.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0060"/>
    <dbReference type="EnsemblBacteria" id="AEF95621">
        <property type="protein sequence ID" value="AEF95621"/>
        <property type="gene ID" value="Metig_0060"/>
    </dbReference>
    <dbReference type="GeneID" id="10642895"/>
    <dbReference type="KEGG" id="mig:Metig_0060"/>
    <dbReference type="HOGENOM" id="CLU_025113_3_1_2"/>
    <dbReference type="OMA" id="CDFDFIG"/>
    <dbReference type="OrthoDB" id="47919at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004821">
        <property type="term" value="F:histidine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006427">
        <property type="term" value="P:histidyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00773">
        <property type="entry name" value="HisRS-like_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.800">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00127">
        <property type="entry name" value="His_tRNA_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00125">
        <property type="entry name" value="HisZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004154">
        <property type="entry name" value="Anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036621">
        <property type="entry name" value="Anticodon-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015807">
        <property type="entry name" value="His-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041715">
        <property type="entry name" value="HisRS-like_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004516">
        <property type="entry name" value="HisRS/HisZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004517">
        <property type="entry name" value="HisZ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43707">
        <property type="entry name" value="PTHR43707"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03129">
        <property type="entry name" value="HGTP_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13393">
        <property type="entry name" value="tRNA-synt_His"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001549">
        <property type="entry name" value="His-tRNA_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="SSF55681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00442">
        <property type="entry name" value="hisS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00443">
        <property type="entry name" value="hisZ_biosyn_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="6 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="4 8 10" id="KW-0436">Ligase</keyword>
    <keyword evidence="5 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="AA_TRNA_LIGASE_II" evidence="9">
        <location>
            <begin position="6"/>
            <end position="330"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AEF95621.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="414" mass="47807" checksum="E878D8879A5C365E" modified="2011-07-27" version="1">MFQKPRGTRDFTPEEMKKRRIIENKLRKVIESYNYKEILTPTFEHFDLIAKKTGEEIRKQLFVFKDHGGREMALRPELTSPVARFYINELKMLPKPLKLYYFANCFRYERPQAGRYREFWQMGCELIGSKSPLADAEIINLAIEGLKSINMDFEVHIGHLGVLRGVFDEMGLDVETQNKIRHLIDKEDIEGLEKFLNEKIGEEKKDIIFKVLEYKGGREILEKVKEDLKDYPKAIEAVENLEEILDFVRHDYVINLGIARGLDYYTGMVFEIYGKREGARQVCGGGRYDNLIETFGGEPTPAVGFAYGFDRIMLNIDDFEVEEEAILITPVKKDKELIKACLDIADTLRKNNKIVEVELMGRRLNKALDYANTKGIKKVIIVGSRELSEGKVTLKDMISGEQKLVDIDKISEVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEH2</accession>
    <name>F6BEH2_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Cellulase</fullName>
            <ecNumber evidence="9">3.2.1.4</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Metig_0072</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
        <text evidence="8">Binds 2 divalent metal cations per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the peptidase M42 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.4" evidence="9"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95633.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798242.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0072"/>
    <dbReference type="MEROPS" id="M42.004"/>
    <dbReference type="EnsemblBacteria" id="AEF95633">
        <property type="protein sequence ID" value="AEF95633"/>
        <property type="gene ID" value="Metig_0072"/>
    </dbReference>
    <dbReference type="GeneID" id="10642907"/>
    <dbReference type="KEGG" id="mig:Metig_0072"/>
    <dbReference type="HOGENOM" id="CLU_047249_0_2_2"/>
    <dbReference type="OMA" id="YTLDNKA"/>
    <dbReference type="OrthoDB" id="27966at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004177">
        <property type="term" value="F:aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008810">
        <property type="term" value="F:cellulase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008007">
        <property type="entry name" value="Peptidase_M42"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023367">
        <property type="entry name" value="Peptidase_M42_dom2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05343">
        <property type="entry name" value="Peptidase_M42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001123">
        <property type="entry name" value="PepA_GA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="9" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Proton acceptor" evidence="7">
        <location>
            <position position="207"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="62"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="175"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="208"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="230"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="321"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR001123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR001123-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR001123-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AEF95633.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="350" mass="38927" checksum="E47E55883F672B1C" modified="2011-07-27" version="1">MRVVEYLKKLAEEKGISGREHRVRELMKKELEPMVDEIYVDKFGNLIARKGDKGPKIMLAAHMDEIGLMVKYIDEKGFLKFTKIGGINDQMILNQKVIVHGNKGDIIGVLGSKPPHKMKESERNKLIKYEDMFIDIGAESREEAIEMGVEIGTWVTFKSEFNELGKNRFTCKAFDDRVGCAVLLEVMRRIKDEDLNCQVYAVGTVQEEVGLKGARTSAFGINPDVALALDVTICGDHPGIKMEDAPVELGKGPVVGIVDAAGRGLITHPKVLDMIRDISKKYDIPVQYEVGEGGTTDATAIHLTRDGIPTGVISIPARYIHTPVEVIDKRDVEKTVELVVSCIKDLDKYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEH5</accession>
    <name>F6BEH5_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Extracellular ligand-binding receptor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0075</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95636.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798245.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0075"/>
    <dbReference type="EnsemblBacteria" id="AEF95636">
        <property type="protein sequence ID" value="AEF95636"/>
        <property type="gene ID" value="Metig_0075"/>
    </dbReference>
    <dbReference type="GeneID" id="10642910"/>
    <dbReference type="KEGG" id="mig:Metig_0075"/>
    <dbReference type="HOGENOM" id="CLU_027128_5_0_2"/>
    <dbReference type="OMA" id="GAQYWKG"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028081">
        <property type="entry name" value="Leu-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13458">
        <property type="entry name" value="Peripla_BP_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="SSF53822"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0675">Receptor</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peripla_BP_6" evidence="1">
        <location>
            <begin position="26"/>
            <end position="364"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF95636.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="387" mass="42004" checksum="4991A76204D45BE4" modified="2011-07-27" version="1">MGLLAIATILCGCVDQNKEAGGAEKEVKVGVILDQTGDLGPIGEKMLKAAELAKDELDGKEIDGKKIKVILIERDSQTQPDAAVKAFNDLVNLEGVKIIVGPMTSSETLAIANLANAKKVVVISPSATLAKISSKEVDPNNFIFRTVGSDALQGRALADLATNLGFKKIVILAINNDYGLGMAEEIEKNFKGEAKTIPYNPNAADYKAEIQQIKEYNPDAVILVGYVESATKLLKDARIAGIKAQWLSSEGIADKAMFKDPEVAEYMVGMIGTRPYSPKDTETYKKFAEAYKAKFGFEPGLFCDTTYDATKLAILAVAKAESYDGEAIKKALYEVSKDYIGASGKKTFDENGDVPQDYEIWKVVKVDGEYDIKVVGKWSEGQITWNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEH6</accession>
    <name>F6BEH6_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Geranylgeranyl reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0076</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95637.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798246.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0076"/>
    <dbReference type="EnsemblBacteria" id="AEF95637">
        <property type="protein sequence ID" value="AEF95637"/>
        <property type="gene ID" value="Metig_0076"/>
    </dbReference>
    <dbReference type="GeneID" id="10642911"/>
    <dbReference type="KEGG" id="mig:Metig_0076"/>
    <dbReference type="HOGENOM" id="CLU_024648_0_1_2"/>
    <dbReference type="OMA" id="IGVHMDR"/>
    <dbReference type="OrthoDB" id="115808at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016628">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-CH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002938">
        <property type="entry name" value="FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011777">
        <property type="entry name" value="Geranylgeranyl_Rdtase_fam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01494">
        <property type="entry name" value="FAD_binding_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02032">
        <property type="entry name" value="GG-red-SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD_binding_3" evidence="1">
        <location>
            <begin position="129"/>
            <end position="309"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF95637.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="383" mass="43267" checksum="708287C71EED602D" modified="2011-07-27" version="1">MDLEKYDVVIVGGGPIGCITGESIKNHNVLIVEEHQSIGVPLQCAGLISKKGVEELGNPKGCVNKIRGAYIHAKNTTVNIGNEEIRAYVFERKVMDKDIAIRASKRVDFLLKTYGRIKIEENKYKLILNHMGEEFEISPKVIIGADGAKSTIGKSVGLVNNKNREILSSCQFEMVNVNVDDDFVHVFIDKDYSKEFFIWIIPMGKDRVRVGLCDKGNCYKKLLDFINNHPTAKEILKNATITEFSVGTLPIGYSKTVKNNVLLVGDAAGQVKPLTGGGLYYGAKCAKICGKIVDEYLSNDYDLNYLKNYEKLWKKEIGREIDRGLKIRKIFLKMSNNQIDKLLNFIKNSGLVEYINEYGDMDKPSDVVRYVFENYFKGFLKFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEJ6</accession>
    <name>F6BEJ6_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">SirA-like domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0096</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95657.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798266.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0096"/>
    <dbReference type="EnsemblBacteria" id="AEF95657">
        <property type="protein sequence ID" value="AEF95657"/>
        <property type="gene ID" value="Metig_0096"/>
    </dbReference>
    <dbReference type="GeneID" id="10642931"/>
    <dbReference type="KEGG" id="mig:Metig_0096"/>
    <dbReference type="HOGENOM" id="CLU_165255_1_2_2"/>
    <dbReference type="OMA" id="NDMASWA"/>
    <dbReference type="OrthoDB" id="124510at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.110.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001455">
        <property type="entry name" value="TusA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036868">
        <property type="entry name" value="TusA-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01206">
        <property type="entry name" value="TusA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64307">
        <property type="entry name" value="SSF64307"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01148">
        <property type="entry name" value="UPF0033"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UPF0033" evidence="1">
        <location>
            <begin position="4"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS01148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF95657.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="72" mass="8148" checksum="E619364540600F8A" modified="2011-07-27" version="1">METLDARGLQCPMPIVELAKKMKELKEGEVLELIADDMGAKEDVPAWCKRTGNELIEMSEEGGVLRFKIRKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEK1</accession>
    <name>F6BEK1_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CGGC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0101</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95662.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798271.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF95662">
        <property type="protein sequence ID" value="AEF95662"/>
        <property type="gene ID" value="Metig_0101"/>
    </dbReference>
    <dbReference type="GeneID" id="10642936"/>
    <dbReference type="KEGG" id="mig:Metig_0101"/>
    <dbReference type="HOGENOM" id="CLU_147304_3_0_2"/>
    <dbReference type="OMA" id="VGIIRCQ"/>
    <dbReference type="OrthoDB" id="91651at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014925">
        <property type="entry name" value="CGGC_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08821">
        <property type="entry name" value="CGGC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01078">
        <property type="entry name" value="CGGC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CGGC" evidence="1">
        <location>
            <begin position="3"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM01078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF95662.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="106" mass="11471" checksum="FA894D3FE3BBA5A9" modified="2011-07-27" version="1">MTKVAILRCDSAAKTCPAVGCVTAALNKKDTFKDYDNVELVTIITCGGCPGRLGLNQIKQLIEKHGVEVVHFATCMSALKPKCIYAEELKEEVEKMGAKVVIGSHF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2020-06-17" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEL4</accession>
    <name>F6BEL4_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_0114</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95675.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF95675">
        <property type="protein sequence ID" value="AEF95675"/>
        <property type="gene ID" value="Metig_0114"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0114"/>
    <dbReference type="HOGENOM" id="CLU_3194505_0_0_2"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF95675.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="44" mass="4817" checksum="9185659E0DFEB9F7" modified="2011-07-27" version="1">MPLMKVDTGDLRRRTLLSVKSMGGAGVLPERSDGNLKTVRFLCP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEQ2</accession>
    <name>F6BEQ2_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative signal transduction protein with CBS domains</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_1312</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96849.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799446.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1312"/>
    <dbReference type="EnsemblBacteria" id="AEF96849">
        <property type="protein sequence ID" value="AEF96849"/>
        <property type="gene ID" value="Metig_1312"/>
    </dbReference>
    <dbReference type="GeneID" id="10644175"/>
    <dbReference type="KEGG" id="mig:Metig_1312"/>
    <dbReference type="HOGENOM" id="CLU_926289_0_0_2"/>
    <dbReference type="OMA" id="PKGGYKA"/>
    <dbReference type="OrthoDB" id="89519at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016436">
        <property type="entry name" value="UCP005063_CBS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005104">
        <property type="entry name" value="WHTH_HrcA_DNA-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03444">
        <property type="entry name" value="HrcA_DNA-bdg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005063">
        <property type="entry name" value="UCP005063_CBS_MJ1232"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="SSF54631"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0129">CBS domain</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBS" evidence="2">
        <location>
            <begin position="177"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="2">
        <location>
            <begin position="239"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF96849.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="323" mass="35694" checksum="18F3D791446369BA" modified="2011-07-27" version="1">MELTVIQKEILQELIAIYKEKNRAVKGTEIAIRLNRNPGTIRNQMQALRALNLVDGVPGPKGGYVPTSEAYRVLGLDEEEEIIVPIYKGDERVEGVSVVKIEFDTVTHEKHCSSKIYIRGDTKKFNVGDVLKIGPTYHNKIVIIGKVVGRDDINHILLLDVMGVASVPNLLVGDVGIKKKLYYLKPDDTIKDAAKLLAYNNISGAPVMEGDKLVGILSLHDVALALADGKVDEKVEKIMSKRPITISAKEKIYNALLTMDKYDVGRLIVVDDNNKVVGIITRTDILRLIEGVLTPNVIKKLSDMEPSKCSKCDTQQIETAAKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2020-06-17" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEQ7</accession>
    <name>F6BEQ7_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_1317</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96854.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96854">
        <property type="protein sequence ID" value="AEF96854"/>
        <property type="gene ID" value="Metig_1317"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_1317"/>
    <dbReference type="HOGENOM" id="CLU_3338422_0_0_2"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF96854.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="37" mass="4634" checksum="4CF5AC0FF654AFA0" modified="2011-07-27" version="1">MEMVQEEIKKNEKREKTEEIIKTPILFRFVVPYLFYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>F6BES8</accession>
    <name>F6BES8_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CRISPR system single-strand-specific deoxyribonuclease Cas10/Csm1 (subtype III-A)</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Cyclic oligoadenylate synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">Metig_1340</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CRISPR-associated Cas10/Csm1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96875.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799472.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1340"/>
    <dbReference type="EnsemblBacteria" id="AEF96875">
        <property type="protein sequence ID" value="AEF96875"/>
        <property type="gene ID" value="Metig_1340"/>
    </dbReference>
    <dbReference type="GeneID" id="10644203"/>
    <dbReference type="KEGG" id="mig:Metig_1340"/>
    <dbReference type="HOGENOM" id="CLU_017487_0_0_2"/>
    <dbReference type="OMA" id="DYFFKGY"/>
    <dbReference type="OrthoDB" id="21880at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004527">
        <property type="term" value="F:exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09680">
        <property type="entry name" value="Cas10_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013408">
        <property type="entry name" value="Cas10/Csm1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041062">
        <property type="entry name" value="Csm1_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000160">
        <property type="entry name" value="GGDEF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006674">
        <property type="entry name" value="HD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18211">
        <property type="entry name" value="Csm1_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01966">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02578">
        <property type="entry name" value="cas_TM1811_Csm1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50887">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0051">Antiviral defense</keyword>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="7" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0540">Nuclease</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GGDEF" evidence="12">
        <location>
            <begin position="545"/>
            <end position="703"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="65"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00014333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00032922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AEF96875.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="835" mass="97300" checksum="018667BDB402596F" modified="2011-07-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F6BES9</accession>
    <name>F6BES9_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CRISPR system Cms protein Csm2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">CRISPR type III A-associated protein Csm2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Metig_1341</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">This subunit may be involved in monitoring complementarity of crRNA and target RNA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CRISPR-associated Csm2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96876.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799473.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1341"/>
    <dbReference type="EnsemblBacteria" id="AEF96876">
        <property type="protein sequence ID" value="AEF96876"/>
        <property type="gene ID" value="Metig_1341"/>
    </dbReference>
    <dbReference type="GeneID" id="25395059"/>
    <dbReference type="KEGG" id="mig:Metig_1341"/>
    <dbReference type="HOGENOM" id="CLU_121766_0_0_2"/>
    <dbReference type="OrthoDB" id="101269at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09647">
        <property type="entry name" value="Csm2_III-A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010149">
        <property type="entry name" value="CRISPR-assoc_prot_Csm2_III-A"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03750">
        <property type="entry name" value="Csm2_III-A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01870">
        <property type="entry name" value="cas_TM1810_Csm2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0051">Antiviral defense</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0694">RNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00031723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEF96876.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="203" mass="24038" checksum="7958F2C67AA02CCA" modified="2011-07-27" version="1">MRNDNKQIKIDNNIYKELKEISEKIQSKEVLNPISLSSKIEKVSKYFKNKKKEKDKILEKFWNEELSRQFSAPICANFADGWKEEDIIKKAMLLSIYLRLDGLKSSQLRKFLDLATNVHNLIKYQKHQKNLHEEILPRIIKMRYILAYQAGKERTVEKLYAVLDPMLQILQKADDNNFKKNFGKFYEFLQAIVAYHKFFGGAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>F6BET3</accession>
    <name>F6BET3_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">O-phospho-L-seryl-tRNA:Cys-tRNA synthase</fullName>
            <ecNumber evidence="2">2.5.1.73</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">Sep-tRNA:Cys-tRNA synthase</fullName>
            <shortName evidence="2">SepCysS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_1345</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Converts O-phospho-L-seryl-tRNA(Cys) (Sep-tRNA(Cys)) to L-cysteinyl-tRNA(Cys) (Cys-tRNA(Cys)).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>H(+) + hydrogen sulfide + O-phospho-L-seryl-tRNA(Cys) = L-cysteinyl-tRNA(Cys) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:25686"/>
            <dbReference type="Rhea" id="RHEA-COMP:9679"/>
            <dbReference type="Rhea" id="RHEA-COMP:9719"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29919"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:78517"/>
            <dbReference type="ChEBI" id="CHEBI:78551"/>
            <dbReference type="EC" id="2.5.1.73"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 2">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer. Interacts with SepRS.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SepCysS family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.73" evidence="2"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96880.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1345"/>
    <dbReference type="EnsemblBacteria" id="AEF96880">
        <property type="protein sequence ID" value="AEF96880"/>
        <property type="gene ID" value="Metig_1345"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_1345"/>
    <dbReference type="HOGENOM" id="CLU_060476_0_0_2"/>
    <dbReference type="OMA" id="HKSMAAS"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043766">
        <property type="term" value="F:Sep-tRNA:Cys-tRNA synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01675">
        <property type="entry name" value="Sep_Cys_tRNA_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013375">
        <property type="entry name" value="Sep_Cys-tRNA_synth_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008829">
        <property type="entry name" value="SepSecS/SepCysS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43586:SF3">
        <property type="entry name" value="PTHR43586:SF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05889">
        <property type="entry name" value="SepSecS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02539">
        <property type="entry name" value="SepCysS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="2" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Pyridoxal phosphate binding" evidence="2">
        <location>
            <begin position="82"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Pyridoxal phosphate binding" evidence="2">
        <location>
            <begin position="211"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="188"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="2">
        <location>
            <position position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF96880.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="376" mass="42598" checksum="52625633DBAB2F22" modified="2011-07-27" version="1">MDKYKNLTRNFERGIINLNPIQRGGILPVEARKAVYEFWDGYSVCDYCGGRLDEISTPPICEFLEDFSKFVNLDISRPTHGAREGKFIVMHSICNEGDYVVVDGNAHYTTYVSAERARLNVVEVESEGYPTFRINPEKYKEVIDNLEDKGKNIGLILLTHVDGNYGNVSDAKKVGKIAKEKGYPFLLNCAYSVGRMPVDGKKLNADFIVASGHKSMAASGPCGVLAFRDEFAEKILKTSSKFPVKEIEMLGCTSRGLPIVTLMASFPHVVERVKRWDEEVKKARYVVEELEKIGFKQLGVKPKEHDLTKFETPVLDEIAKKDKRRGFFFYEELKKRGIGGIKRGVTKEIKMSVYGLTWEQVEYVVNAIKEIVETHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEU4</accession>
    <name>F6BEU4_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0119</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95680.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0119"/>
    <dbReference type="EnsemblBacteria" id="AEF95680">
        <property type="protein sequence ID" value="AEF95680"/>
        <property type="gene ID" value="Metig_0119"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0119"/>
    <dbReference type="HOGENOM" id="CLU_086260_0_0_2"/>
    <dbReference type="OMA" id="GYWEQHP"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002749">
        <property type="entry name" value="DUF63"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40700">
        <property type="entry name" value="PTHR40700"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01889">
        <property type="entry name" value="DUF63"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="29"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="57"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="87"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="114"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="142"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="181"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="212"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF95680.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="269" mass="30836" checksum="000F21853430A154" modified="2011-07-27" version="1">MLSAIKEFIYEYYIKPMIEGSGYNLVQEITYGIFLTFMVYIFYKACVKLRVAIDEKFAQTTVFYVVLISLMRALVDAGVIERSFFTITPGIVILIGSYYMASILISGVLLRERYYKLAIPFALLPIIYFLPDFLNRIVHWEALLYVSLILFPTYILTVFIIKKTKIENKIISSKIDKYAIFSQLVDASATAVGIGIYGYWEQHPIPRFFMDMFGAYVMIPLKLAVVLIALYLINEEVDNKDLKNILKITIMCLGLAPGLRNLLRTIMGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEZ2</accession>
    <name>F6BEZ2_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Iron-sulfur cluster carrier protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metig_0171</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Binds and transfers iron-sulfur (Fe-S) clusters to target apoproteins. Can hydrolyze ATP.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the Mrp/NBP35 ATP-binding proteins family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95728.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798337.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0171"/>
    <dbReference type="EnsemblBacteria" id="AEF95728">
        <property type="protein sequence ID" value="AEF95728"/>
        <property type="gene ID" value="Metig_0171"/>
    </dbReference>
    <dbReference type="GeneID" id="10643006"/>
    <dbReference type="KEGG" id="mig:Metig_0171"/>
    <dbReference type="HOGENOM" id="CLU_024839_0_1_2"/>
    <dbReference type="OMA" id="NMAYFTP"/>
    <dbReference type="OrthoDB" id="32313at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140663">
        <property type="term" value="F:ATP-dependent FeS chaperone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02040">
        <property type="entry name" value="Mrp_NBP35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019591">
        <property type="entry name" value="Mrp/NBP35_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033756">
        <property type="entry name" value="YlxH/NBP35"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23264">
        <property type="entry name" value="PTHR23264"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10609">
        <property type="entry name" value="ParA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="47"/>
            <end position="54"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEF95728.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="289" mass="30974" checksum="3D263334034D8160" modified="2011-07-27" version="1">MSECDGKCDTCSLKNSCPDTKKIIEQQNARIRENMGKIKHKIVILSGKGGVGKSTVTVNLAAALNMMGKKVGVLDADIHGPNIPKMFGVEGLQPMASPAGIFPITTPQGIKTISIEYFLPSESTPVVWRGPKVSGAVRQFLSDVVWGELDYLLIDTPPGSGDVQLTILQSIPDIDGAIIVTTPEDVAVLDAKKSITMAKMLNVPILGIIENMSGFVCPHCNKVVDVFGKGGGEKAAKEFGAEFLGRIPLDVKAREASDKGIPMVLLDCKASEEFKKIVNRITEKIEGKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>F6BF84</accession>
    <name>F6BF84_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Nitrogenase iron protein</fullName>
            <ecNumber evidence="12">1.18.6.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Nitrogenase Fe protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Nitrogenase component II</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Nitrogenase reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">nifH</name>
        <name evidence="15" type="ordered locus">Metig_1419</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 12">The key enzymatic reactions in nitrogen fixation are catalyzed by the nitrogenase complex, which has 2 components: the iron protein and the molybdenum-iron protein.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 12">
            <text>16 ATP + 16 H2O + N2 + 8 reduced [2Fe-2S]-[ferredoxin] = 16 ADP + 6 H(+) + H2 + 2 NH4(+) + 8 oxidized [2Fe-2S]-[ferredoxin] + 16 phosphate</text>
            <dbReference type="Rhea" id="RHEA:21448"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17997"/>
            <dbReference type="ChEBI" id="CHEBI:18276"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="1.18.6.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="12">Binds 1 [4Fe-4S] cluster per dimer.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4 12">Homodimer.</text>
    </comment>
    <comment type="PTM">
        <text evidence="13">The reversible ADP-ribosylation of Arg inactivates the nitrogenase reductase and regulates nitrogenase activity.</text>
    </comment>
    <comment type="PTM">
        <text evidence="12">The reversible ADP-ribosylation of Arg-109 inactivates the nitrogenase reductase and regulates nitrogenase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 12 14">Belongs to the NifH/BchL/ChlL family.</text>
    </comment>
    <dbReference type="EC" id="1.18.6.1" evidence="12"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96954.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799550.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1419"/>
    <dbReference type="EnsemblBacteria" id="AEF96954">
        <property type="protein sequence ID" value="AEF96954"/>
        <property type="gene ID" value="Metig_1419"/>
    </dbReference>
    <dbReference type="GeneID" id="10644292"/>
    <dbReference type="KEGG" id="mig:Metig_1419"/>
    <dbReference type="HOGENOM" id="CLU_059373_0_0_2"/>
    <dbReference type="OMA" id="YVCDYYL"/>
    <dbReference type="OrthoDB" id="66372at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018697">
        <property type="term" value="F:carbonyl sulfide nitrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016163">
        <property type="term" value="F:nitrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009399">
        <property type="term" value="P:nitrogen fixation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00533">
        <property type="entry name" value="NifH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030655">
        <property type="entry name" value="NifH/chlL_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000392">
        <property type="entry name" value="NifH/frxC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005977">
        <property type="entry name" value="Nitrogenase_Fe_NifH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42864">
        <property type="entry name" value="PTHR42864"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00142">
        <property type="entry name" value="Fer4_NifH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000363">
        <property type="entry name" value="Nitrogenase_iron"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01287">
        <property type="entry name" value="nifH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00746">
        <property type="entry name" value="NIFH_FRXC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00692">
        <property type="entry name" value="NIFH_FRXC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51026">
        <property type="entry name" value="NIFH_FRXC_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 14" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="7 12 13" id="KW-0013">ADP-ribosylation</keyword>
    <keyword evidence="8 12 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 12 14" id="KW-0408">Iron</keyword>
    <keyword evidence="10 12 14" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="5 12 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 12" id="KW-0535">Nitrogen fixation</keyword>
    <keyword evidence="6 12 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12 14 15" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="8"/>
            <end position="15"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="106"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="142"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="modified residue" description="ADP-ribosylarginine; by dinitrogenase reductase ADP-ribosyltransferase" evidence="12 13">
        <location>
            <position position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR605977-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU003688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="AEF96954.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="290" mass="31590" checksum="FA4211A919BAE583" modified="2011-07-27" version="1">MKQIAFYGKGGIGKSTTVCNIAAALADEGKRVMVVGCDPKHDCTSNLRGGEEIPTVLDTLREKGMEKLSLEDAIEGKKIEMDEIVYKGYKGIYCIEAGGPKPGYGCAGRGVIVAIDLLKKMNVFEELGVDVVLYDVLGDVVCGGFAMPLRMGLAEQIYIVTSSDYMAMYAANNICRGMKEFAKRGGSRLGGLIYNVRGSLDAEDIVTEFAKKLGTEIIGKIPNSLLIAEAEIEGKTVIEYAPDSEIASIYRELAKKIYKNKNGVIPNPLENKELMQIGLAIKERIKKMRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>F6BA71</accession>
    <name>F6BA71_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized conserved protein UCP006577</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0204</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95761.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798370.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0204"/>
    <dbReference type="EnsemblBacteria" id="AEF95761">
        <property type="protein sequence ID" value="AEF95761"/>
        <property type="gene ID" value="Metig_0204"/>
    </dbReference>
    <dbReference type="GeneID" id="10643039"/>
    <dbReference type="KEGG" id="mig:Metig_0204"/>
    <dbReference type="HOGENOM" id="CLU_159086_1_0_2"/>
    <dbReference type="OMA" id="TKPCYGT"/>
    <dbReference type="OrthoDB" id="100968at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012031">
        <property type="entry name" value="UCP006577"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08979">
        <property type="entry name" value="DUF1894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006577">
        <property type="entry name" value="UCP006577"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="83"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF95761.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="110" mass="12843" checksum="D5E692B2B24D4BD6" modified="2011-07-27" version="1">MSCIDKYNYEILFRGSFKECAKFIRENCKNIKEVNPGEEVIEGVMLIGIPPIPVGYEEDCIIIPYTKPCYGTFVLKVKIEEEKDKKESKENKENEDKKDKKGILSKLKFW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>F6BA79</accession>
    <name>F6BA79_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">CRISPR-associated helicase, Cyano-type</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0212</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95769.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798378.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0212"/>
    <dbReference type="EnsemblBacteria" id="AEF95769">
        <property type="protein sequence ID" value="AEF95769"/>
        <property type="gene ID" value="Metig_0212"/>
    </dbReference>
    <dbReference type="GeneID" id="10643047"/>
    <dbReference type="KEGG" id="mig:Metig_0212"/>
    <dbReference type="HOGENOM" id="CLU_414273_0_0_2"/>
    <dbReference type="OMA" id="EYHLYDE"/>
    <dbReference type="OrthoDB" id="5372at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017575">
        <property type="entry name" value="CRISPR-assoc_helicase_CYANO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03158">
        <property type="entry name" value="cas3_cyano"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="1">
        <location>
            <begin position="36"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF95769.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="662" mass="78787" checksum="6024983E1343F63E" modified="2011-07-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>F6BA84</accession>
    <name>F6BA84_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="3">NADH dehydrogenase (Quinone)</fullName>
            <ecNumber evidence="3">1.6.99.5</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_0217</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.6.99.5" evidence="3"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95774.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798383.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0217"/>
    <dbReference type="EnsemblBacteria" id="AEF95774">
        <property type="protein sequence ID" value="AEF95774"/>
        <property type="gene ID" value="Metig_0217"/>
    </dbReference>
    <dbReference type="GeneID" id="10643052"/>
    <dbReference type="KEGG" id="mig:Metig_0217"/>
    <dbReference type="HOGENOM" id="CLU_007100_9_5_2"/>
    <dbReference type="OMA" id="YVAHHIT"/>
    <dbReference type="OrthoDB" id="28394at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001750">
        <property type="entry name" value="ND/Mrp_mem"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00361">
        <property type="entry name" value="Proton_antipo_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="30"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="73"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="100"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="125"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="155"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="196"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="237"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="263"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="291"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="315"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="350"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="398"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="441"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="domain" description="Proton_antipo_M" evidence="2">
        <location>
            <begin position="119"/>
            <end position="405"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF95774.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="481" mass="52457" checksum="F12D3D4A8D680FF1" modified="2011-07-27" version="1">MNFLPLIVVFPLMMAIILNYLHGKEKIVRFLTFLVALALIVLPFIGEYGFYYFSGHGVENGLISGIAYLFNQTKQVVIFTLMLIGSLVLITGMGEKHANGMFTALTLMGLASVSAVVLSDDLFNLYVFYEIAAVAQTGLVIASGTEKAYKAAFRYMLMGCVAGSLLLLGIAFLLSATGTLNITDMKNYLINANPMVYGGLLMLIIGLTYGSGLPPFHTVKADLYARAKPFIAAMLQTYSKFVLVAMMLVILKLFYGMPYFNTAHGALIALSIFGMVFGVVMALMQSDYRKLLAYHAISQGGYVAAGLALGTPLGIVAGIFHAINHVIYKSALFLGAYIVHKKKGSNLAKLGGLLPIIPSVAFMVLCAKLAISGVPPFNGFQSKWMLAEAAMEVNMPELAVIMIIVSIGTFVSMMKAFYLIYLKPCSEEQINEYKSKEVPKLAIFSLAVLTFLCILLGIYPDIVVEKLYPYAYEIGRTWALK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAB9</accession>
    <name>F6BAB9_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">D-glycero-beta-D-manno-heptose 1-phosphate adenylyltransferase</fullName>
            <ecNumber evidence="2">2.7.7.70</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Metig_0253</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + D-glycero-beta-D-manno-heptose 1-phosphate + H(+) = ADP-D-glycero-beta-D-manno-heptose + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:27465"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:59967"/>
            <dbReference type="ChEBI" id="CHEBI:61593"/>
            <dbReference type="EC" id="2.7.7.70"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.7.70" evidence="2"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95809.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0253"/>
    <dbReference type="EnsemblBacteria" id="AEF95809">
        <property type="protein sequence ID" value="AEF95809"/>
        <property type="gene ID" value="Metig_0253"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0253"/>
    <dbReference type="HOGENOM" id="CLU_034585_2_0_2"/>
    <dbReference type="OMA" id="KGTDYTP"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016773">
        <property type="term" value="F:phosphotransferase activity, alcohol group as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009058">
        <property type="term" value="P:biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004821">
        <property type="entry name" value="Cyt_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011914">
        <property type="entry name" value="RfaE_dom_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01467">
        <property type="entry name" value="CTP_transf_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00125">
        <property type="entry name" value="cyt_tran_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02199">
        <property type="entry name" value="rfaE_dom_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="CTP_transf_like" evidence="6">
        <location>
            <begin position="38"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEF95809.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="169" mass="19424" checksum="02894F2D1792A5FF" modified="2011-07-27" version="1">MTNINILEIRDNMIIKNRTLLKDIIKELKSQNLKIVFTNGCFDIIHRGHVEYLNKAKKLGDVLIVGINSDESIKKIKGEKRPIIPLYSRAYVLDNLKPVDFVVPFDEDSPIELIKIIKPHIHVKGGDYKEEDLPEAEVVKSYGGEVKIIPLIEGFSTTNIIEWILEKYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAC3</accession>
    <name>F6BAC3_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Metallophosphoesterase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0257</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95813.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798422.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0257"/>
    <dbReference type="EnsemblBacteria" id="AEF95813">
        <property type="protein sequence ID" value="AEF95813"/>
        <property type="gene ID" value="Metig_0257"/>
    </dbReference>
    <dbReference type="GeneID" id="10643092"/>
    <dbReference type="KEGG" id="mig:Metig_0257"/>
    <dbReference type="HOGENOM" id="CLU_074761_0_1_2"/>
    <dbReference type="OMA" id="DEYLFPW"/>
    <dbReference type="OrthoDB" id="82715at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024654">
        <property type="entry name" value="Calcineurin-like_PHP_lpxH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011152">
        <property type="entry name" value="Pesterase_MJ0912"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12850">
        <property type="entry name" value="Metallophos_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000883">
        <property type="entry name" value="Pesterase_MJ0912"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="SSF56300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metallophos_2" evidence="1">
        <location>
            <begin position="1"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF95813.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="240" mass="27225" checksum="B10385E9C1BC04DA" modified="2011-07-27" version="1">MKLAILSDIHSNLEALEAVLNDIDKHDIDRVFCLGDIVGYGANPNECIEIIKKFPTVAGNHDYGVIGKEMISFFNTFGQIAIIYTKEVLKEENAEYLKKLPLIIEEKIDGKKVIFSHACPQNPDSWEYLFPDYVPDYVFSYEDVVFVGHSHIPFVYSKNRGCVLTHEGKIELNENDKYVINPGSVGQPRDGVNRASYAIFDTDEYSVEIKRIPYDIIKAANKILNVGLPEYLAERLFLGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAC8</accession>
    <name>F6BAC8_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Dihydroorotate dehydrogenase</fullName>
            <shortName evidence="8">DHOD</shortName>
            <shortName evidence="8">DHODase</shortName>
            <shortName evidence="8">DHOdehase</shortName>
            <ecNumber evidence="8">1.3.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">pyrD</name>
        <name evidence="10" type="ordered locus">Metig_0262</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the conversion of dihydroorotate to orotate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>(S)-dihydroorotate + A = AH2 + orotate</text>
            <dbReference type="Rhea" id="RHEA:18073"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:30839"/>
            <dbReference type="ChEBI" id="CHEBI:30864"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
        <text evidence="8">Binds 1 FMN per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 8">Pyrimidine metabolism; UMP biosynthesis via de novo pathway.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the dihydroorotate dehydrogenase family. Type 1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="1.3.-.-" evidence="8"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95818.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798427.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0262"/>
    <dbReference type="EnsemblBacteria" id="AEF95818">
        <property type="protein sequence ID" value="AEF95818"/>
        <property type="gene ID" value="Metig_0262"/>
    </dbReference>
    <dbReference type="GeneID" id="10643097"/>
    <dbReference type="KEGG" id="mig:Metig_0262"/>
    <dbReference type="HOGENOM" id="CLU_042042_0_1_2"/>
    <dbReference type="OMA" id="SCPHAKG"/>
    <dbReference type="OrthoDB" id="31316at2157"/>
    <dbReference type="UniPathway" id="UPA00070"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004152">
        <property type="term" value="F:dihydroorotate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006207">
        <property type="term" value="P:'de novo' pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044205">
        <property type="term" value="P:'de novo' UMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd04740">
        <property type="entry name" value="DHOD_1B_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00224">
        <property type="entry name" value="DHO_dh_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033888">
        <property type="entry name" value="DHOD_1B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005720">
        <property type="entry name" value="Dihydroorotate_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024920">
        <property type="entry name" value="Dihydroorotate_DH_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012135">
        <property type="entry name" value="Dihydroorotate_DH_1_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001295">
        <property type="entry name" value="Dihydroorotate_DH_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01180">
        <property type="entry name" value="DHO_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000164">
        <property type="entry name" value="DHO_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01037">
        <property type="entry name" value="pyrD_sub1_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00911">
        <property type="entry name" value="DHODEHASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00912">
        <property type="entry name" value="DHODEHASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 8" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6 8" id="KW-0288">FMN</keyword>
    <keyword evidence="7 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DHO_dh" evidence="9">
        <location>
            <begin position="1"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="8">
        <location>
            <begin position="67"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="8">
        <location>
            <begin position="192"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="8">
        <location>
            <position position="129"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="43"/>
            <end position="44"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="43"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="126"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="165"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="191"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="218"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="244"/>
            <end position="245"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="266"/>
            <end position="267"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AEF95818.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="304" mass="32564" checksum="BB38C232FE1E661B" modified="2011-07-27" version="1">MLKTVICGIEFKNPVFLAAGVMGETGSALKRIAKNGAGAVCTKSVGLERKEGHKNPTIVEVEGGFLNAMGLPNPGVDEYVEEIERVRNDLKRMNVRIIGSIYGKDEREFAIVAEKIEPYVDMIELNISCPHAGGGYGATIGQNPDLSYKVVESVKDAVKIPVFAKLTPNVTDIKVIAKAVVEAGADGITAINTLGPGMVIDIEARKPILSNKFGGMSGKAIKPIAVKIVYDLYETVDVPIIGVGGITTGKDALEFIMAGASAVQVGTGVYYRGYDIFRKICEEMEVFLKEKNVKLKDLVGCAHD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2020-06-17" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAD9</accession>
    <name>F6BAD9_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_0273</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95829.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF95829">
        <property type="protein sequence ID" value="AEF95829"/>
        <property type="gene ID" value="Metig_0273"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0273"/>
    <dbReference type="HOGENOM" id="CLU_2597740_0_0_2"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF95829.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="79" mass="9172" checksum="CFFE2A2A143F6140" modified="2011-07-27" version="1">MELGVISERSDENLKTVRFLCPRQGQRHSCVADDAQVQYGYLLTNTHIGQTPKIGEYLLKIKHHIEYLGCREVEYSRVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAE5</accession>
    <name>F6BAE5_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1423</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96958.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799554.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1423"/>
    <dbReference type="EnsemblBacteria" id="AEF96958">
        <property type="protein sequence ID" value="AEF96958"/>
        <property type="gene ID" value="Metig_1423"/>
    </dbReference>
    <dbReference type="GeneID" id="10644296"/>
    <dbReference type="KEGG" id="mig:Metig_1423"/>
    <dbReference type="HOGENOM" id="CLU_134280_4_0_2"/>
    <dbReference type="OMA" id="IAINERY"/>
    <dbReference type="OrthoDB" id="120805at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019277">
        <property type="entry name" value="DUF2304"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10066">
        <property type="entry name" value="DUF2304"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="33"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="68"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96958.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="116" mass="13639" checksum="E0620C0E69DDBD01" modified="2011-07-27" version="1">MEIIQIFGILFALFALSRVILQIRNRNMNLDEGIFWIFVWTLVVVVLVFPQTLDYLARVLGVGRGVDAIIYLSIVALFYLIYRMYAKMEHLEREITKVVREIAIKDRYEPKDKNEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAE6</accession>
    <name>F6BAE6_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Aspartate carbamoyltransferase</fullName>
            <ecNumber evidence="5">2.1.3.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Aspartate transcarbamylase</fullName>
            <shortName evidence="5">ATCase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">pyrB</name>
        <name evidence="9" type="ordered locus">Metig_1424</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 5">
            <text>carbamoyl phosphate + L-aspartate = H(+) + N-carbamoyl-L-aspartate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20013"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:32814"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
            <dbReference type="EC" id="2.1.3.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 5">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 2/3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Heterooligomer of catalytic and regulatory chains.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the aspartate/ornithine carbamoyltransferase superfamily. ATCase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.3.2" evidence="5"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96959.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799555.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1424"/>
    <dbReference type="EnsemblBacteria" id="AEF96959">
        <property type="protein sequence ID" value="AEF96959"/>
        <property type="gene ID" value="Metig_1424"/>
    </dbReference>
    <dbReference type="GeneID" id="10644297"/>
    <dbReference type="KEGG" id="mig:Metig_1424"/>
    <dbReference type="HOGENOM" id="CLU_043846_1_2_2"/>
    <dbReference type="OMA" id="FPTEREY"/>
    <dbReference type="OrthoDB" id="51351at2157"/>
    <dbReference type="UniPathway" id="UPA00070">
        <property type="reaction ID" value="UER00116"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016597">
        <property type="term" value="F:amino acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004070">
        <property type="term" value="F:aspartate carbamoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006207">
        <property type="term" value="P:'de novo' pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044205">
        <property type="term" value="P:'de novo' UMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006520">
        <property type="term" value="P:cellular amino acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1370">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00001">
        <property type="entry name" value="Asp_carb_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006132">
        <property type="entry name" value="Asp/Orn_carbamoyltranf_P-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006130">
        <property type="entry name" value="Asp/Orn_carbamoylTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036901">
        <property type="entry name" value="Asp/Orn_carbamoylTrfase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002082">
        <property type="entry name" value="Asp_carbamoyltransf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006131">
        <property type="entry name" value="Asp_carbamoyltransf_Asp/Orn-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11405:SF16">
        <property type="entry name" value="PTHR11405:SF16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00185">
        <property type="entry name" value="OTCace"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02729">
        <property type="entry name" value="OTCace_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00100">
        <property type="entry name" value="AOTCASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53671">
        <property type="entry name" value="SSF53671"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00670">
        <property type="entry name" value="asp_carb_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00097">
        <property type="entry name" value="CARBAMOYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="OTCace_N" evidence="8">
        <location>
            <begin position="2"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="domain" description="OTCace" evidence="7">
        <location>
            <begin position="151"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AEF96959.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="303" mass="34757" checksum="3B8B69BD40E5DFD9" modified="2011-07-27" version="1">MRHLISMRDIGKEDILRILDEAEKMEEILNSKKPSKILEGKILATIFYEPSTRTRLSFETAMKRLGGEVIGFGDIENTSVAKGESLIDTIRVVSNYCDIIVLRHPAEGAARLASEYSSVPIINAGDGSNQHPTQTLLDLYTIKREIGRIDNIRVAFVGDLKYGRTVHSLSYALALFEGVELHFISPKELKMPREIIEDLKGRVKIYESDKIDDLDVDVVYVTRIQKERFPDLNEYQKVKGSYKIKREHVENKDLIVMHPLPRVDEIDYEVDKLPQAKYFKQSFYGIPVRMAILKILIEEFEKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAF8</accession>
    <name>F6BAF8_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Parallel beta-helix repeat protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1436</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96971.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1436"/>
    <dbReference type="EnsemblBacteria" id="AEF96971">
        <property type="protein sequence ID" value="AEF96971"/>
        <property type="gene ID" value="Metig_1436"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_1436"/>
    <dbReference type="HOGENOM" id="CLU_227230_0_0_2"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004197">
        <property type="term" value="F:cysteine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.10">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.3800">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011626">
        <property type="entry name" value="Alpha-macroglobulin_TED"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039448">
        <property type="entry name" value="Beta_helix"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006633">
        <property type="entry name" value="Carb-bd_sugar_hydrolysis-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011635">
        <property type="entry name" value="CARDB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029030">
        <property type="entry name" value="Caspase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001769">
        <property type="entry name" value="Gingipain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038490">
        <property type="entry name" value="Gingipain_propep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007742">
        <property type="entry name" value="NosD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022441">
        <property type="entry name" value="Para_beta_helix_rpt-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006626">
        <property type="entry name" value="PbH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012334">
        <property type="entry name" value="Pectin_lyas_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012600">
        <property type="entry name" value="Propeptide_C25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008930">
        <property type="entry name" value="Terpenoid_cyclase/PrenylTrfase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13229">
        <property type="entry name" value="Beta_helix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07705">
        <property type="entry name" value="CARDB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05048">
        <property type="entry name" value="NosD"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01364">
        <property type="entry name" value="Peptidase_C25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08126">
        <property type="entry name" value="Propeptide_C25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07678">
        <property type="entry name" value="TED_complement"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00722">
        <property type="entry name" value="CASH"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00710">
        <property type="entry name" value="PbH1"/>
        <property type="match status" value="24"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48239">
        <property type="entry name" value="SSF48239"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="SSF51126"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52129">
        <property type="entry name" value="SSF52129"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03804">
        <property type="entry name" value="para_beta_helix"/>
        <property type="match status" value="9"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CASH" evidence="1">
        <location>
            <begin position="695"/>
            <end position="832"/>
        </location>
    </feature>
    <feature type="domain" description="CASH" evidence="1">
        <location>
            <begin position="1253"/>
            <end position="1423"/>
        </location>
    </feature>
    <feature type="domain" description="CASH" evidence="1">
        <location>
            <begin position="1491"/>
            <end position="1627"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96971.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="2719" mass="303735" checksum="959AB729AD52BC38" modified="2011-07-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAI6</accession>
    <name>F6BAI6_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1464</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96999.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1464"/>
    <dbReference type="EnsemblBacteria" id="AEF96999">
        <property type="protein sequence ID" value="AEF96999"/>
        <property type="gene ID" value="Metig_1464"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_1464"/>
    <dbReference type="HOGENOM" id="CLU_197251_0_0_2"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="5"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96999.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="62" mass="6812" checksum="17C9A9A3707BDE00" modified="2011-07-27" version="1">MEIGILVASSIVVATIASYYYITNYLNSHPENAGKSANKTIIKINKTVYSEVKDLNDTSTGW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAJ6</accession>
    <name>F6BAJ6_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcriptional regulator, LysR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1475</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97009.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1475"/>
    <dbReference type="EnsemblBacteria" id="AEF97009">
        <property type="protein sequence ID" value="AEF97009"/>
        <property type="gene ID" value="Metig_1475"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_1475"/>
    <dbReference type="HOGENOM" id="CLU_992530_0_0_2"/>
    <dbReference type="OMA" id="AHRIVWK"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000847">
        <property type="entry name" value="Tscrpt_reg_HTH_LysR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00126">
        <property type="entry name" value="HTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50931">
        <property type="entry name" value="HTH_LYSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH lysR-type" evidence="1">
        <location>
            <begin position="20"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF97009.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="290" mass="33308" checksum="E0DB41A2249E0534" modified="2011-07-27" version="1">MSGKEEFNPTINIEYKGKIITSNQINILKAILRTKSQNKAAKMLKVPPSSINIQIKRLESKLNTKLIYSSPSGTMLTEEGIRIIDYFNSIQNRLNEGEFIACGFVSGEIGRILFDDVIISSFDNVLKLYNMGLTKIIGVDDPYWSFRVMDSRFLRSEGGLGVESEGEVYPVAYDYFVMVYKDEFDFKNLVGIRYSAQRIVWKILKNEGISFKITKCVKNPFQAIESVEEGYSLFLNKSFSKYINNYNIEEPKFYEKTKHTINFIGDLEGMNELIEKKKKEIKNAGFEVIC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAN1</accession>
    <name>F6BAN1_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">4Fe-4S ferredoxin iron-sulfur binding domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0289</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95845.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798454.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0289"/>
    <dbReference type="EnsemblBacteria" id="AEF95845">
        <property type="protein sequence ID" value="AEF95845"/>
        <property type="gene ID" value="Metig_0289"/>
    </dbReference>
    <dbReference type="GeneID" id="10643124"/>
    <dbReference type="KEGG" id="mig:Metig_0289"/>
    <dbReference type="HOGENOM" id="CLU_043374_3_2_2"/>
    <dbReference type="OMA" id="DMQRCIG"/>
    <dbReference type="OrthoDB" id="105930at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13247">
        <property type="entry name" value="Fer4_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12800">
        <property type="entry name" value="Fer4_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="2"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="80"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF95845.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="167" mass="17983" checksum="7DB1CB2F1AFC568A" modified="2011-07-27" version="1">MKSIFVNPERCIGCKSCQIQCAVSHSGKDSIFEAIYAEPTPKPRIFVEYMGVAVPLQCRHCENAPCMAACPSNAIKKEDGAVVVEKEKCIGCKACVMVCPFGAMSIDVGESKVLKCDLCGGNPACVSACKTGALQYGDILELIKEKRKKYLKSMLKIVAIGGVHECK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAT0</accession>
    <name>F6BAT0_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Holliday junction resolvase Hjc</fullName>
            <shortName evidence="10">Hjc</shortName>
            <ecNumber evidence="10">3.1.21.10</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">hjc</name>
        <name evidence="11" type="ordered locus">Metig_0338</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">A structure-specific endonuclease that resolves Holliday junction (HJ) intermediates during genetic recombination. Cleaves 4-way DNA junctions introducing paired nicks in opposing strands, leaving a 5'-terminal phosphate and a 3'-terminal hydroxyl group that are ligated to produce recombinant products.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction).</text>
            <dbReference type="EC" id="3.1.21.10"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="10">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the Holliday junction resolvase Hjc family.</text>
    </comment>
    <dbReference type="EC" id="3.1.21.10" evidence="10"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95894.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798503.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0338"/>
    <dbReference type="EnsemblBacteria" id="AEF95894">
        <property type="protein sequence ID" value="AEF95894"/>
        <property type="gene ID" value="Metig_0338"/>
    </dbReference>
    <dbReference type="GeneID" id="10643175"/>
    <dbReference type="KEGG" id="mig:Metig_0338"/>
    <dbReference type="HOGENOM" id="CLU_139546_2_0_2"/>
    <dbReference type="OMA" id="WYFFHPG"/>
    <dbReference type="OrthoDB" id="97278at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008821">
        <property type="term" value="F:crossover junction endodeoxyribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00523">
        <property type="entry name" value="archeal_HJR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1350.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01490">
        <property type="entry name" value="HJ_Resolv_Hjc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002732">
        <property type="entry name" value="Hjc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014428">
        <property type="entry name" value="Hjc_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011335">
        <property type="entry name" value="Restrct_endonuc-II-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011856">
        <property type="entry name" value="tRNA_endonuc-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39651">
        <property type="entry name" value="PTHR39651"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01870">
        <property type="entry name" value="Hjc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004985">
        <property type="entry name" value="Hlld_jn_rslvs_ar"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52980">
        <property type="entry name" value="SSF52980"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10" id="KW-0227">DNA damage</keyword>
    <keyword evidence="8 10" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="9 10" id="KW-0234">DNA repair</keyword>
    <keyword evidence="7 10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 10" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="5 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1 10" id="KW-0540">Nuclease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" evidence="10">
        <location>
            <position position="29"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="9"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="33"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="46"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="10">
        <location>
            <position position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AEF95894.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="128" mass="14678" checksum="A9C433D46C9F37B9" modified="2011-07-27" version="1">MYRKGSNFERELKRALEKKGFAVVRSAGSHGVDIIAGKNGNIYIFECKSTSKEKFYVKKEDIDKLISFSETFGGKPYLALKIKGKWLFINPYLLHTDGKNYALEYKKIQVIAIDFKELVGEGKQVKFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>F6BB18</accession>
    <name>F6BB18_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="3">RNA methyltransferase, TrmH family, group 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_1571</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the class IV-like SAM-binding methyltransferase superfamily. RNA methyltransferase TrmH family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97105.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799699.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1571"/>
    <dbReference type="EnsemblBacteria" id="AEF97105">
        <property type="protein sequence ID" value="AEF97105"/>
        <property type="gene ID" value="Metig_1571"/>
    </dbReference>
    <dbReference type="GeneID" id="10644444"/>
    <dbReference type="KEGG" id="mig:Metig_1571"/>
    <dbReference type="HOGENOM" id="CLU_056931_3_0_2"/>
    <dbReference type="OMA" id="PIMNLSH"/>
    <dbReference type="OrthoDB" id="93855at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008173">
        <property type="term" value="F:RNA methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1280.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029028">
        <property type="entry name" value="Alpha/beta_knot_MTases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004384">
        <property type="entry name" value="RNA_MeTrfase_TrmJ/LasT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001537">
        <property type="entry name" value="SpoU_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029026">
        <property type="entry name" value="tRNA_m1G_MTases_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42786">
        <property type="entry name" value="PTHR42786"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00588">
        <property type="entry name" value="SpoU_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004808">
        <property type="entry name" value="LasT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75217">
        <property type="entry name" value="SSF75217"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00050">
        <property type="entry name" value="rRNA_methyl_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SpoU_methylase" evidence="2">
        <location>
            <begin position="4"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF97105.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="236" mass="26899" checksum="DB6ABD5B734FF0C9" modified="2011-07-27" version="1">MEPIVIFVNPKYSGNVGAIARNMKNFGVKELRIVGNRDILDKEAYIRAVHAKDILDNAKFYNSLKDAIEDIDFVVATSGAIGGDRNLKRVPITPREFAKRSLEIEGKVGIVFGREDDGLTNEELELCDLLVSIPTSEEYPIMNVSHAVAVILYELYVTRVEHEKEIPYNVKMRVASKLEKEILIKLFNEFVDKSNIPDYRKDLCKVIFKRIISRAFISGKEANTLMCVFRDKLKKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>F6BB65</accession>
    <name>F6BB65_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 10">Indole-3-glycerol phosphate synthase</fullName>
            <shortName evidence="10">IGPS</shortName>
            <ecNumber evidence="4 10">4.1.1.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">trpC</name>
        <name evidence="12" type="ordered locus">Metig_0394</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate + H(+) = (1S,2R)-1-C-(indol-3-yl)glycerol 3-phosphate + CO2 + H2O</text>
            <dbReference type="Rhea" id="RHEA:23476"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:58613"/>
            <dbReference type="ChEBI" id="CHEBI:58866"/>
            <dbReference type="EC" id="4.1.1.48"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 10">Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 4/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the TrpC family.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.48" evidence="4 10"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95950.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798559.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0394"/>
    <dbReference type="EnsemblBacteria" id="AEF95950">
        <property type="protein sequence ID" value="AEF95950"/>
        <property type="gene ID" value="Metig_0394"/>
    </dbReference>
    <dbReference type="GeneID" id="10643231"/>
    <dbReference type="KEGG" id="mig:Metig_0394"/>
    <dbReference type="HOGENOM" id="CLU_034247_2_0_2"/>
    <dbReference type="OMA" id="REIVWQK"/>
    <dbReference type="OrthoDB" id="98284at2157"/>
    <dbReference type="UniPathway" id="UPA00035">
        <property type="reaction ID" value="UER00043"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004425">
        <property type="term" value="F:indole-3-glycerol-phosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000162">
        <property type="term" value="P:tryptophan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00331">
        <property type="entry name" value="IGPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00134_A">
        <property type="entry name" value="IGPS_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045186">
        <property type="entry name" value="Indole-3-glycerol_P_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013798">
        <property type="entry name" value="Indole-3-glycerol_P_synth_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001468">
        <property type="entry name" value="Indole-3-GlycerolPSynthase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22854">
        <property type="entry name" value="PTHR22854"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00218">
        <property type="entry name" value="IGPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="SSF51366"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00614">
        <property type="entry name" value="IGPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="8 10" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="10" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="9 10 12" id="KW-0456">Lyase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 10" id="KW-0822">Tryptophan biosynthesis</keyword>
    <feature type="domain" description="IGPS" evidence="11">
        <location>
            <begin position="4"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001633"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00018080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AEF95950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="268" mass="30707" checksum="7F5A60D8FA3B8D52" modified="2011-07-27" version="1">MNVLDKIVKDTRKRIELERGRNILRELENFIKDRELDEYIEKHSKLKLSKSIKKAKETRNPIITEIKPSSPSKGNIRRIKEEDIENIARDMVEGGCCGISILTEPKYFNGSYRNLIIARKFKVPILFKDFVVDFYQIDIAKTIGANIVLLMVSVLGEDIGEFLDYAHENDLECLVETHSEEEIDIALDSNAKIIGINNRDLKTLNIDLKTTEKLAPLIPNKKIKISESGIYTRDDLMYVLKYTNCALIGSSIMESGNIKEKVEELSRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>F6BB66</accession>
    <name>F6BB66_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 15">Fructose-1,6-bisphosphate aldolase/phosphatase</fullName>
            <shortName evidence="15">FBP A/P</shortName>
            <shortName evidence="15">FBP aldolase/phosphatase</shortName>
            <ecNumber evidence="6 15">3.1.3.11</ecNumber>
            <ecNumber evidence="15">4.1.2.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">fbp</name>
        <name evidence="16" type="ordered locus">Metig_0395</name>
    </gene>
    <organism evidence="17">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Catalyzes two subsequent steps in gluconeogenesis: the aldol condensation of dihydroxyacetone phosphate (DHAP) and glyceraldehyde-3-phosphate (GA3P) to fructose-1,6-bisphosphate (FBP), and the dephosphorylation of FBP to fructose-6-phosphate (F6P).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 15">
            <text>beta-D-fructose 1,6-bisphosphate + H2O = beta-D-fructose 6-phosphate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:11064"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57634"/>
            <dbReference type="EC" id="3.1.3.11"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>beta-D-fructose 1,6-bisphosphate = D-glyceraldehyde 3-phosphate + dihydroxyacetone phosphate</text>
            <dbReference type="Rhea" id="RHEA:14729"/>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="4.1.2.13"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 15">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 15">Carbohydrate biosynthesis; gluconeogenesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5 15">Homooctamer; dimer of tetramers.</text>
    </comment>
    <comment type="domain">
        <text evidence="15">Consists of a single catalytic domain, but remodels its active-site architecture via a large structural change to exhibit dual activities.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 15">Belongs to the FBP aldolase/phosphatase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.11" evidence="6 15"/>
    <dbReference type="EC" id="4.1.2.13" evidence="15"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95951.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798560.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0395"/>
    <dbReference type="EnsemblBacteria" id="AEF95951">
        <property type="protein sequence ID" value="AEF95951"/>
        <property type="gene ID" value="Metig_0395"/>
    </dbReference>
    <dbReference type="GeneID" id="10643232"/>
    <dbReference type="KEGG" id="mig:Metig_0395"/>
    <dbReference type="HOGENOM" id="CLU_041630_0_0_2"/>
    <dbReference type="OMA" id="YMRRHGP"/>
    <dbReference type="OrthoDB" id="21175at2157"/>
    <dbReference type="UniPathway" id="UPA00138"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042132">
        <property type="term" value="F:fructose 1,6-bisphosphate 1-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004332">
        <property type="term" value="F:fructose-bisphosphate aldolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02067">
        <property type="entry name" value="FBP_aldolase_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002803">
        <property type="entry name" value="FBPase_V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036076">
        <property type="entry name" value="FBPase_V_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38341">
        <property type="entry name" value="PTHR38341"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01950">
        <property type="entry name" value="FBPase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015647">
        <property type="entry name" value="FBPtase_archl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111249">
        <property type="entry name" value="SSF111249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14 15" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="8 15" id="KW-0312">Gluconeogenesis</keyword>
    <keyword evidence="10 15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12 15" id="KW-0456">Lyase</keyword>
    <keyword evidence="11 15" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13 15" id="KW-0704">Schiff base</keyword>
    <feature type="region of interest" description="FBP binding" evidence="15">
        <location>
            <begin position="105"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="region of interest" description="FBP binding; shared with dimeric partner" evidence="15">
        <location>
            <begin position="243"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor; for FBP phosphatase activity" evidence="15">
        <location>
            <position position="14"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor; for FBP aldolase activity" evidence="15">
        <location>
            <position position="230"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with DHAP; for FBP aldolase activity" evidence="15">
        <location>
            <position position="233"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="14"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="21"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="54"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="55"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="96"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="133"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="134"/>
        </location>
        <ligand>
            <name>dihydroxyacetone phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="233"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="234"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="235"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="267"/>
        </location>
        <ligand>
            <name>dihydroxyacetone phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="288"/>
        </location>
        <ligand>
            <name>dihydroxyacetone phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00018635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="AEF95951.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="383" mass="42384" checksum="A44AB14496E5DC84" modified="2011-07-27" version="1">MDNNKITVSVIKADVGGLCGHTLAPEDLLEACEGVLEEAVDELIIDYYVTRCGDDVDLIMTHKMGVDNEKIHKLAWNAFEEATKIAKELKLYGAGQDLLAECFSGNVRGLGPGCAEMEFVERPSEPIVVFCCDKTDPAAFNLPLYKIFADPFNTAGLVFDPSMTSGFAFEVHDVIGSKKVILNTPEETYSLLALIGDVERYAIKRVYRRRDNEIAAVVSTEKLNYIAGEYVGKDDPVAVVRAQSGFPAVGEILEPFANPHFVAGWMRGSHWGPLMPVGEEDATPSRFDGPARIMALGFQLADGMLIGPNDLFADKGFDKAREKALEMADIIRRMGPFQPHRLPSGMMEYTTVPKVLEKLKDRFEPLDDEPKKLEKCRDRGDVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>F6BB72</accession>
    <name>F6BB72_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">EamA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_0401</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95957.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0401"/>
    <dbReference type="EnsemblBacteria" id="AEF95957">
        <property type="protein sequence ID" value="AEF95957"/>
        <property type="gene ID" value="Metig_0401"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0401"/>
    <dbReference type="HOGENOM" id="CLU_1870747_0_0_2"/>
    <dbReference type="OMA" id="YKGKASI"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000620">
        <property type="entry name" value="EamA_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00892">
        <property type="entry name" value="EamA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="33"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="65"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="93"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="120"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="2">
        <location>
            <begin position="4"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF95957.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="140" mass="15278" checksum="1F594A37D492D113" modified="2011-07-27" version="1">MISIGVIIGLLTALFFGIGTFLAKIVCEKDPIFQWIVVNIVGIILCIAILAKYHQKLQVFEWKILLYAVSSAIMIVLGSLLLYYGLYKGKASIVVPLSSIGPAITVILAVIFLKEQLTTNQIMGIFLILLGIILISINQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>F6BB83</accession>
    <name>F6BB83_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">NAD kinase</fullName>
            <ecNumber evidence="5">2.7.1.23</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">ATP-dependent NAD kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">nadK</name>
        <name evidence="6" type="ordered locus">Metig_0412</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Involved in the regulation of the intracellular balance of NAD and NADP, and is a key enzyme in the biosynthesis of NADP. Catalyzes specifically the phosphorylation on 2'-hydroxyl of the adenosine moiety of NAD to yield NADP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>ATP + NAD(+) = ADP + H(+) + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:18629"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.23"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the NAD kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.23" evidence="5"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95968.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798577.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0412"/>
    <dbReference type="EnsemblBacteria" id="AEF95968">
        <property type="protein sequence ID" value="AEF95968"/>
        <property type="gene ID" value="Metig_0412"/>
    </dbReference>
    <dbReference type="GeneID" id="10643249"/>
    <dbReference type="KEGG" id="mig:Metig_0412"/>
    <dbReference type="HOGENOM" id="CLU_445249_0_0_2"/>
    <dbReference type="OMA" id="VIVPICP"/>
    <dbReference type="OrthoDB" id="23762at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003951">
        <property type="term" value="F:NAD+ kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046855">
        <property type="term" value="P:inositol phosphate dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006553">
        <property type="term" value="P:lysine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019674">
        <property type="term" value="P:NAD metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006741">
        <property type="term" value="P:NADP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00361">
        <property type="entry name" value="NAD_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017438">
        <property type="entry name" value="ATP-NAD_kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017437">
        <property type="entry name" value="ATP-NAD_kinase_PpnK-typ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021175">
        <property type="entry name" value="Bifunctional_PpnK_predicted"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000760">
        <property type="entry name" value="Inositol_monophosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016064">
        <property type="entry name" value="NAD/diacylglycerol_kinase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002504">
        <property type="entry name" value="NADK"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00459">
        <property type="entry name" value="Inositol_P"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01513">
        <property type="entry name" value="NAD_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036641">
        <property type="entry name" value="Bifunctional_PpnK_predicted"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00377">
        <property type="entry name" value="IMPHPHTASES"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111331">
        <property type="entry name" value="SSF111331"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 5 6" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 5" id="KW-0520">NAD</keyword>
    <keyword evidence="3 5" id="KW-0521">NADP</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 5 6" id="KW-0808">Transferase</keyword>
    <feature type="active site" description="Proton acceptor" evidence="5">
        <location>
            <position position="367"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="367"/>
            <end position="368"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="372"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="442"/>
            <end position="443"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="453"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="470"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="472"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="483"/>
            <end position="488"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEF95968.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="579" mass="63982" checksum="CCB605DF7441909F" modified="2011-07-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>F6BB90</accession>
    <name>F6BB90_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 4">Probable thymidylate kinase</fullName>
            <ecNumber evidence="2 4">2.7.4.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">dTMP kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">tmk</name>
        <name evidence="6" type="ordered locus">Metig_0419</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>ATP + dTMP = ADP + dTDP</text>
            <dbReference type="Rhea" id="RHEA:13517"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58369"/>
            <dbReference type="ChEBI" id="CHEBI:63528"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.9"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the thymidylate kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.9" evidence="2 4"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95975.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0419"/>
    <dbReference type="EnsemblBacteria" id="AEF95975">
        <property type="protein sequence ID" value="AEF95975"/>
        <property type="gene ID" value="Metig_0419"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0419"/>
    <dbReference type="HOGENOM" id="CLU_049131_1_3_2"/>
    <dbReference type="OMA" id="VMTREPG"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004798">
        <property type="term" value="F:thymidylate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006233">
        <property type="term" value="P:dTDP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006235">
        <property type="term" value="P:dTTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00165">
        <property type="entry name" value="Thymidylate_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039430">
        <property type="entry name" value="Thymidylate_kin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018095">
        <property type="entry name" value="Thymidylate_kin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018094">
        <property type="entry name" value="Thymidylate_kinase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02223">
        <property type="entry name" value="Thymidylate_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00041">
        <property type="entry name" value="DTMP_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01331">
        <property type="entry name" value="THYMIDYLATE_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 6" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0545">Nucleotide biosynthesis</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Thymidylate_kin" evidence="5">
        <location>
            <begin position="9"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="11"/>
            <end position="18"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEF95975.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="198" mass="22824" checksum="CE815EA53C82C2BB" modified="2011-07-27" version="1">MFKRMFIVFEGIDGSGKTTQAKLLTKKLNAFYTCEPTEGQIGRLIREILKGKKKCEKETLALLFAADRVEHVKLIEEKLKESHVVCDRYLYSSIAYQTSQGVDEDFILQINRFAKKPDIVVLLTVDVEEGMKRLGKEKEIFEKEEFLKKVQNKYLKLAKKEGFIVIDTTNKSINESHEEILKIIKSKLWGLSNMSIGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>F6BB97</accession>
    <name>F6BB97_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Rubredoxin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Metig_0426</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 7">Rubredoxin is a small nonheme, iron protein lacking acid-labile sulfide. Its single Fe, chelated to 4 Cys, functions as an electron acceptor and may also stabilize the conformation of the molecule.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7 8">
            <name>Fe(3+)</name>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
        </cofactor>
        <text evidence="7 8">Binds 1 Fe(3+) ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the rubredoxin family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95982.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798591.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0426"/>
    <dbReference type="EnsemblBacteria" id="AEF95982">
        <property type="protein sequence ID" value="AEF95982"/>
        <property type="gene ID" value="Metig_0426"/>
    </dbReference>
    <dbReference type="GeneID" id="10643263"/>
    <dbReference type="KEGG" id="mig:Metig_0426"/>
    <dbReference type="HOGENOM" id="CLU_128747_3_1_2"/>
    <dbReference type="OMA" id="APKDMFE"/>
    <dbReference type="OrthoDB" id="126197at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00730">
        <property type="entry name" value="rubredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024922">
        <property type="entry name" value="Rubredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024934">
        <property type="entry name" value="Rubredoxin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024935">
        <property type="entry name" value="Rubredoxin_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00301">
        <property type="entry name" value="Rubredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000071">
        <property type="entry name" value="Rubredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00163">
        <property type="entry name" value="RUBREDOXIN"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50903">
        <property type="entry name" value="RUBREDOXIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0249">Electron transport</keyword>
    <keyword evidence="6 7 8" id="KW-0408">Iron</keyword>
    <keyword evidence="4 7 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Rubredoxin-like" evidence="9">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="6"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="8"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="38"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="41"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR000071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000071-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AEF95982.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="51" mass="5727" checksum="53AC830706267B47" modified="2011-07-27" version="1">MDKYQCMCGWIYDPEVGEPSQNIKPGTPFEALPDTFTCPQCGLGKNAFRKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBB8</accession>
    <name>F6BBB8_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Transposase, IS605 OrfB family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Metig_1592</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">In the C-terminal section; belongs to the transposase 35 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97125.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799718.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1592"/>
    <dbReference type="EnsemblBacteria" id="AEF97125">
        <property type="protein sequence ID" value="AEF97125"/>
        <property type="gene ID" value="Metig_1592"/>
    </dbReference>
    <dbReference type="GeneID" id="10644465"/>
    <dbReference type="KEGG" id="mig:Metig_1592"/>
    <dbReference type="HOGENOM" id="CLU_032903_3_4_2"/>
    <dbReference type="OMA" id="WENTTWK"/>
    <dbReference type="OrthoDB" id="75656at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032196">
        <property type="term" value="P:transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001959">
        <property type="entry name" value="Transposase_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010095">
        <property type="entry name" value="Transposase_IS605_OrfB_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01385">
        <property type="entry name" value="OrfB_IS605"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07282">
        <property type="entry name" value="OrfB_Zn_ribbon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01766">
        <property type="entry name" value="tspaseT_teng_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0815">Transposition</keyword>
    <feature type="domain" description="OrfB_IS605" evidence="5">
        <location>
            <begin position="193"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="domain" description="OrfB_Zn_ribbon" evidence="6">
        <location>
            <begin position="304"/>
            <end position="365"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF07282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEF97125.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="412" mass="48368" checksum="E8FF0D729AB1AB88" modified="2011-07-27" version="1">MPNKTKSKGTQELSYQVVLSYKVSHNYPLKTFLIECKDKLNECIDMIWNNIEYTKKDKPKLPKSNEFKRNLRNKLLENWSYASHYIDGIIKTSYSILQSWASNYKRGYRTKTKPVVERLFVRVKTTLIKYDKERAEIRITIKPRKEYLTLNIKNEWFFDKVKDLTIGEIILKENEAFLTFKDNLNYSDKDIIVGVDSNLKSLDLFHPIEGWIRIDLTELHRLKEVYDKKIDGLKKLLKKCPLRVLRKINRLFERRRNRVKDFLHKLTTQLSRLFPDAIFVFEDLNKRRMYKNKHFNRRIDRANWNGIIEKISYKAVVILVNPAYTSTTCPICGSRMESQEGQVVCYNCSNSFNRQVVGCFNIFKRGLGAIKRFMGGSGVTTTGAEVSAKGLMTPNPNVVYYVDLNGKYLKCI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBD6</accession>
    <name>F6BBD6_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1610</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97143.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799736.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1610"/>
    <dbReference type="EnsemblBacteria" id="AEF97143">
        <property type="protein sequence ID" value="AEF97143"/>
        <property type="gene ID" value="Metig_1610"/>
    </dbReference>
    <dbReference type="GeneID" id="10644483"/>
    <dbReference type="KEGG" id="mig:Metig_1610"/>
    <dbReference type="HOGENOM" id="CLU_1623471_0_0_2"/>
    <dbReference type="OMA" id="FMGNIVC"/>
    <dbReference type="OrthoDB" id="80296at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011314">
        <property type="entry name" value="Prd_NiFe_hyd_3_EhaB"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF019706">
        <property type="entry name" value="EhaB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="70"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="103"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="129"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF97143.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="163" mass="17440" checksum="63CA84C26667D046" modified="2011-07-27" version="1">MLEVLKIAIASIVSWINFVLIDTYFGLPEKPGVLGAKVIGEKVRDIGGNLNGGYFMGNIVCSPDASAGTLLASICYYLMGIEGGLIAALFVYIGNRLCHDPGYAGTIGALMATVLIHLFSPIIEPKYFIVGMVIAIFSIQGFDHVLASKVLGEIARKMNRTGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBF2</accession>
    <name>F6BBF2_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">4Fe-4S ferredoxin iron-sulfur binding domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1626</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97159.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799752.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1626"/>
    <dbReference type="EnsemblBacteria" id="AEF97159">
        <property type="protein sequence ID" value="AEF97159"/>
        <property type="gene ID" value="Metig_1626"/>
    </dbReference>
    <dbReference type="GeneID" id="10644499"/>
    <dbReference type="KEGG" id="mig:Metig_1626"/>
    <dbReference type="HOGENOM" id="CLU_097041_1_0_2"/>
    <dbReference type="OMA" id="VLCGHCE"/>
    <dbReference type="OrthoDB" id="111853at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13237">
        <property type="entry name" value="Fer4_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12838">
        <property type="entry name" value="Fer4_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="26"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="56"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="104"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="133"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF97159.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="160" mass="18135" checksum="FD033FEA804DD61D" modified="2011-07-27" version="1">MLNDILKKYFNGEEIIPNFNIKASMKKIEVNEEKCIACNRCVEVCPVNAIEPNTPFPVEIDEKKCIYCGKCVEVCPVNAINIFKASAKIEDGELKIEKNIKKYKELIYNRKKCCLCLVCLKTCPFGAIFEDKSTLKFDMKKCTLCGHCGEVCPLDAIEFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBJ0</accession>
    <name>F6BBJ0_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Methyl-coenzyme M reductase operon protein D</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0443</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95999.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798608.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0443"/>
    <dbReference type="EnsemblBacteria" id="AEF95999">
        <property type="protein sequence ID" value="AEF95999"/>
        <property type="gene ID" value="Metig_0443"/>
    </dbReference>
    <dbReference type="GeneID" id="10643280"/>
    <dbReference type="KEGG" id="mig:Metig_0443"/>
    <dbReference type="HOGENOM" id="CLU_118415_0_0_2"/>
    <dbReference type="OMA" id="NFPYGYD"/>
    <dbReference type="OrthoDB" id="94905at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015948">
        <property type="term" value="P:methanogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003901">
        <property type="entry name" value="Me_CoM_Rdtase_D"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02505">
        <property type="entry name" value="MCR_D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005636">
        <property type="entry name" value="McrD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03260">
        <property type="entry name" value="met_CoM_red_D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0484">Methanogenesis</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF95999.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="178" mass="20533" checksum="E6B4B67790976027" modified="2011-07-27" version="1">MEENGIEVVDIKIFPHRYLKPETSEKVLNAIYDLGDDIIRVIVHGPSLPKYVYYGPARGMPVNHSDRKVIKIKGEEVELTVKVGEIIVTVPFEKAMEIEKKLHKICKENFPYGYDIYVGAFTKVRPTVTDYLKYGERFVNEVDKRLIGITDPRSKFESAVKIIPKEEKEDEEKSSKEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBL4</accession>
    <name>F6BBL4_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">UPF0313 protein Metig_0467</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Metig_0467</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="6">Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the UPF0313 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96023.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798631.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96023">
        <property type="protein sequence ID" value="AEF96023"/>
        <property type="gene ID" value="Metig_0467"/>
    </dbReference>
    <dbReference type="GeneID" id="10643304"/>
    <dbReference type="KEGG" id="mig:Metig_0467"/>
    <dbReference type="HOGENOM" id="CLU_018288_2_0_2"/>
    <dbReference type="OMA" id="TCMYYTG"/>
    <dbReference type="OrthoDB" id="8457at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.30.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01251">
        <property type="entry name" value="UPF0313"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaB/NifB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020612">
        <property type="entry name" value="Methylthiotransferase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023404">
        <property type="entry name" value="rSAM_horseshoe"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022946">
        <property type="entry name" value="UPF0313"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024560">
        <property type="entry name" value="UPF0313_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013704">
        <property type="entry name" value="UPF0313_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32331">
        <property type="entry name" value="PTHR32331"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11842">
        <property type="entry name" value="DUF3362"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08497">
        <property type="entry name" value="Radical_SAM_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01069">
        <property type="entry name" value="UPF0313"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03904">
        <property type="entry name" value="SAM_YgiQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01278">
        <property type="entry name" value="MTTASE_RADICAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 6" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="4 6" id="KW-0408">Iron</keyword>
    <keyword evidence="5 6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="7">
        <location>
            <begin position="286"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="300"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="304"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="307"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEF96023.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="605" mass="70532" checksum="26164711DF46BCBB" modified="2011-07-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBM0</accession>
    <name>F6BBM0_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Formylmethanofuran dehydrogenase</fullName>
            <ecNumber evidence="4">1.2.7.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Metig_0473</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>H2O + N-formylmethanofuran + 2 oxidized [2Fe-2S]-[ferredoxin] = CO2 + H(+) + methanofuran + 2 reduced [2Fe-2S]-[ferredoxin]</text>
            <dbReference type="Rhea" id="RHEA:19841"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:57727"/>
            <dbReference type="ChEBI" id="CHEBI:58151"/>
            <dbReference type="EC" id="1.2.7.12"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">One-carbon metabolism; methanogenesis from CO(2); 5,10-methenyl-5,6,7,8-tetrahydromethanopterin from CO(2): step 1/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the FwdC/FmdC family.</text>
    </comment>
    <dbReference type="EC" id="1.2.7.12" evidence="4"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96029.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798637.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="SMR" id="F6BBM0"/>
    <dbReference type="STRING" id="880724.Metig_0473"/>
    <dbReference type="EnsemblBacteria" id="AEF96029">
        <property type="protein sequence ID" value="AEF96029"/>
        <property type="gene ID" value="Metig_0473"/>
    </dbReference>
    <dbReference type="GeneID" id="10643310"/>
    <dbReference type="KEGG" id="mig:Metig_0473"/>
    <dbReference type="HOGENOM" id="CLU_072248_0_0_2"/>
    <dbReference type="OMA" id="VNGDAGM"/>
    <dbReference type="OrthoDB" id="72252at2157"/>
    <dbReference type="UniPathway" id="UPA00640">
        <property type="reaction ID" value="UER00692"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018493">
        <property type="term" value="F:formylmethanofuran dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046914">
        <property type="term" value="F:transition metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019386">
        <property type="term" value="P:methanogenesis, from carbon dioxide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00980">
        <property type="entry name" value="FwdC/FmdC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017550">
        <property type="entry name" value="Formylmethanofuran_DH_suC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002489">
        <property type="entry name" value="Glu_synth_asu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036485">
        <property type="entry name" value="Glu_synth_asu_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01493">
        <property type="entry name" value="GXGXG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69336">
        <property type="entry name" value="SSF69336"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03122">
        <property type="entry name" value="one_C_dehyd_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GXGXG" evidence="5">
        <location>
            <begin position="72"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004830"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEF96029.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="273" mass="29749" checksum="46B68B1574BC37BF" modified="2011-07-27" version="1">MKELILTLKEDIIVPVEMDAVIPEKIENMTLDEIKNIELPQGNKKVLLKDLFDVELNEDADVSRIIIKNSTIKLKRIGEKMTTGEIIVEGDAGMYVGCEMKGGKIIVNGNADSWAGQNMKGGELIIKGNAKDYVGSAYRGDWRGMSGGKIIVEGNVGNEIGEYMRGGVIHVKGNAGLMAGIHQEGGIIIIEGDVDARVGGEMKKGAIVVYGKVEELLPSFKFEGVVEDPVIKLSKKDEGTPIKGKFYKFSGDYVYNKPKGQLYISVESNPDLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-06-02" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBM8</accession>
    <name>F6BBM8_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_0481</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96037.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798645.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96037">
        <property type="protein sequence ID" value="AEF96037"/>
        <property type="gene ID" value="Metig_0481"/>
    </dbReference>
    <dbReference type="GeneID" id="10643318"/>
    <dbReference type="KEGG" id="mig:Metig_0481"/>
    <dbReference type="HOGENOM" id="CLU_145179_0_0_2"/>
    <dbReference type="OMA" id="NERWTVM"/>
    <dbReference type="OrthoDB" id="137411at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011322">
        <property type="entry name" value="N-reg_PII-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019296">
        <property type="entry name" value="Unchr_N-regulatory-PII-rel"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10126">
        <property type="entry name" value="Nit_Regul_Hom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54913">
        <property type="entry name" value="SSF54913"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF96037.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="107" mass="12224" checksum="0B647465921688FA" modified="2011-07-27" version="1">MLLFKLFVEKDNVGKAINALTEGGITGFFLHECKGISPKKFKVILENLDEVVEAIRELNDAIIIKTVIDKKKAKILEDIIEEKLANERYTIIKIPINKIKINTCKKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-09-29" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBP6</accession>
    <name>F6BBP6_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0499</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96055.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798663.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96055">
        <property type="protein sequence ID" value="AEF96055"/>
        <property type="gene ID" value="Metig_0499"/>
    </dbReference>
    <dbReference type="GeneID" id="10643336"/>
    <dbReference type="KEGG" id="mig:Metig_0499"/>
    <dbReference type="HOGENOM" id="CLU_185767_0_0_2"/>
    <dbReference type="OMA" id="VFFQSHI"/>
    <dbReference type="OrthoDB" id="132064at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="32"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96055.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="96" mass="10934" checksum="65649B16D7C456E6" modified="2011-07-27" version="1">MDVSSILSIIQQHWLEILIILAIGFILRNIKWIAIAVLIFIALSYFGLLDQIKDVIFGQRGIILDFLKNYTSTLINQTINETLNQTINQTISNLTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBR3</accession>
    <name>F6BBR3_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 3">30S ribosomal protein S28e</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">rps28e</name>
        <name evidence="4" type="ordered locus">Metig_1661</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the eukaryotic ribosomal protein eS28 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97193.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799784.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1661"/>
    <dbReference type="EnsemblBacteria" id="AEF97193">
        <property type="protein sequence ID" value="AEF97193"/>
        <property type="gene ID" value="Metig_1661"/>
    </dbReference>
    <dbReference type="GeneID" id="10644534"/>
    <dbReference type="KEGG" id="mig:Metig_1661"/>
    <dbReference type="HOGENOM" id="CLU_178987_2_1_2"/>
    <dbReference type="OMA" id="MHGEASQ"/>
    <dbReference type="OrthoDB" id="121548at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04457">
        <property type="entry name" value="S1_S28E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00292">
        <property type="entry name" value="Ribosomal_S28e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000289">
        <property type="entry name" value="Ribosomal_S28e"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028626">
        <property type="entry name" value="Ribosomal_S28e_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10769">
        <property type="entry name" value="PTHR10769"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01200">
        <property type="entry name" value="Ribosomal_S28e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00961">
        <property type="entry name" value="RIBOSOMAL_S28E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 4" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00029525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEF97193.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="75" mass="8209" checksum="35E8FCAF49AC9A60" modified="2011-07-27" version="1">MSDDMVYKDAVAAEVIQIIGRTGVTGEITQVRCKILGGKDAGRVLTRNIKGPVRVGDIVMLRETEREAKPLGKKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBW2</accession>
    <name>F6BBW2_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1710</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97242.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799831.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1710"/>
    <dbReference type="EnsemblBacteria" id="AEF97242">
        <property type="protein sequence ID" value="AEF97242"/>
        <property type="gene ID" value="Metig_1710"/>
    </dbReference>
    <dbReference type="GeneID" id="10644583"/>
    <dbReference type="KEGG" id="mig:Metig_1710"/>
    <dbReference type="HOGENOM" id="CLU_048986_1_0_2"/>
    <dbReference type="OMA" id="ESMYYTI"/>
    <dbReference type="OrthoDB" id="116265at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007254">
        <property type="entry name" value="DUF373"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38815">
        <property type="entry name" value="PTHR38815"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04123">
        <property type="entry name" value="DUF373"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="166"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="190"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="232"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="256"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="300"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="327"/>
            <end position="350"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF97242.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="359" mass="40518" checksum="4ADE9C46B9190DC2" modified="2011-07-27" version="1">MEIRKLPEVKKYLVLVVDMDDDVGRKANITTPILGREDNIKAAIKLGLSDPGDSDVNAILGGVKLYDELKNKGKDVEIATISGDKDVESEKCALKIKEQLDFLMYLYEPNFIYLVSDGAEDEMVLKYLDSKDIFVWKKRIVIKQNESLESIYYLIQEFIKKTMSDYIPLIFTSAGFAMILYAIFSELGWRIIVGLIGLYVLSEGVGVRKLFIEKIKKGQEGIELGKISPMGTVVALLILTVGALYAYGVSKSDNPVIFAANFLYNISNPLTLSLFVFILGHFIDKIIHSKESILNILKRYFFYTICLFMAREFLIISSEFLKGSESFTMLMVHAVVYTSIIIILSTILFAKSNMQTDTM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBY7</accession>
    <name>F6BBY7_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Tetratricopeptide TPR_2 repeat-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0512</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96068.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798676.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0512"/>
    <dbReference type="EnsemblBacteria" id="AEF96068">
        <property type="protein sequence ID" value="AEF96068"/>
        <property type="gene ID" value="Metig_0512"/>
    </dbReference>
    <dbReference type="GeneID" id="10643349"/>
    <dbReference type="KEGG" id="mig:Metig_0512"/>
    <dbReference type="HOGENOM" id="CLU_1080144_0_0_2"/>
    <dbReference type="OMA" id="SPDKEKW"/>
    <dbReference type="OrthoDB" id="17at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_repeat"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="69"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="148"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="182"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96068.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="257" mass="30170" checksum="5DA4C6D8623E7C54" modified="2011-07-27" version="1">MRIFSKLGFIFICVVVTIAGCVDIPQSSKNSKNEISPNVATAFNMYKEGRYQTALIYCDKAIKENPNDKWAWYLKGKIYYQLNESAKAVECFNKSIRIDPNFADAYFWMGLTYTSYYITNEPSHYAVTCDYKTARKYIGKAIELNPNKDIYYSYMAQCYILDDLDKSIEYINKAIELNPDNAYYWVQKADYLELKGKYKDALLCYDEALKIEPDNVDIMLKKAELYHRMGLTYKEKEILEEVEKIDPQKAELYRFTH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>F6BC01</accession>
    <name>F6BC01_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Nicotinate-nucleotide pyrophosphorylase [carboxylating]</fullName>
            <ecNumber evidence="6">2.4.2.19</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Quinolinate phosphoribosyltransferase [decarboxylating]</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Metig_0527</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Involved in the catabolism of quinolinic acid (QA).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>CO2 + diphosphate + nicotinate beta-D-ribonucleotide = 5-phospho-alpha-D-ribose 1-diphosphate + 2 H(+) + quinolinate</text>
            <dbReference type="Rhea" id="RHEA:12733"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:29959"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57502"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="EC" id="2.4.2.19"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 6">Cofactor biosynthesis; NAD(+) biosynthesis; nicotinate D-ribonucleotide from quinolinate: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Hexamer formed by 3 homodimers.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the NadC/ModD family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.19" evidence="6"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96082.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798690.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0527"/>
    <dbReference type="EnsemblBacteria" id="AEF96082">
        <property type="protein sequence ID" value="AEF96082"/>
        <property type="gene ID" value="Metig_0527"/>
    </dbReference>
    <dbReference type="GeneID" id="10643365"/>
    <dbReference type="KEGG" id="mig:Metig_0527"/>
    <dbReference type="HOGENOM" id="CLU_039622_2_0_2"/>
    <dbReference type="OMA" id="DMIMLKD"/>
    <dbReference type="OrthoDB" id="73201at2157"/>
    <dbReference type="UniPathway" id="UPA00253">
        <property type="reaction ID" value="UER00331"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004514">
        <property type="term" value="F:nicotinate-nucleotide diphosphorylase (carboxylating) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009435">
        <property type="term" value="P:NAD biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd01572">
        <property type="entry name" value="QPRTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1170.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004393">
        <property type="entry name" value="NadC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027277">
        <property type="entry name" value="NadC/ModD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036068">
        <property type="entry name" value="Nicotinate_pribotase-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037128">
        <property type="entry name" value="Quinolinate_PRibosylTase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002638">
        <property type="entry name" value="Quinolinate_PRibosylTrfase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022412">
        <property type="entry name" value="Quinolinate_PRibosylTrfase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32179">
        <property type="entry name" value="PTHR32179"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01729">
        <property type="entry name" value="QRPTase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02749">
        <property type="entry name" value="QRPTase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006250">
        <property type="entry name" value="NadC_ModD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51690">
        <property type="entry name" value="SSF51690"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00078">
        <property type="entry name" value="nadC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6 9" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="3 6" id="KW-0662">Pyridine nucleotide biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="QRPTase_N" evidence="8">
        <location>
            <begin position="18"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="domain" description="QRPTase_C" evidence="7">
        <location>
            <begin position="105"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009400"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR006250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AEF96082.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="281" mass="31724" checksum="864653768ECBA673" modified="2011-07-27" version="1">MIKDYALRILKESLSHDVGFGDITTELIIPKDLACKGVIKSKEECVVCGLDFVVELFKSYGIKCKPLVKEGDLVHGDILEVYGDARTILILERTVLNFLMHLSGIATKTYNVIKKVRQINKNVRIACTRKTLPMLSILEKYAVSVGGGDTHRFRLDDCVMIKDNHIAAVGIEKAFERVKKVSFTKKIEVEVDNLEQLREVLKYKPDIVLLDNFKPEEVGEALKIIDNYEKEHNYRPIVEVSGGIKEDNILDYAKYNVDVISMGCLIHSARAIDISLDVDIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>F6BC23</accession>
    <name>F6BC23_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Periplasmic binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0550</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96104.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0550"/>
    <dbReference type="EnsemblBacteria" id="AEF96104">
        <property type="protein sequence ID" value="AEF96104"/>
        <property type="gene ID" value="Metig_0550"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0550"/>
    <dbReference type="HOGENOM" id="CLU_025776_0_0_2"/>
    <dbReference type="OMA" id="NWYAVGG"/>
    <dbReference type="OrthoDB" id="12936at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002491">
        <property type="entry name" value="ABC_transptr_periplasmic_BD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036439">
        <property type="entry name" value="Dockerin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01497">
        <property type="entry name" value="Peripla_BP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63446">
        <property type="entry name" value="SSF63446"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fe/B12 periplasmic-binding" evidence="1">
        <location>
            <begin position="149"/>
            <end position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96104.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="443" mass="50868" checksum="80C59C1B365F4FA7" modified="2011-07-27" version="1">MVKKILKILSAMLMILLTPIVFGENIGDVNGDGSINIADVVYLFKHRDVGLEKGDLNCDNSVDIADVVYLFNNYQKWREPVVFAKNFEIEYYDANGNPVSYDDNWAYKIITTKYGNEIYNKYCIVREGEEPPSNLPSDVKVIYAPVKKVATLSAFQVAMLEALDNDSVRESIRGVSEYTYTKIKDYGVFPKLKSYVDNGNIVPIGKWGAIDKEALLNISPDIVFLAWTHGYLATDMNTVSDLGMNYLVTVESNEPDFLAKAEWAKVYAAFYNLDKKGNDFLQKVWEKRNELIRKTRNAAYRPKVAMLYWSPYSGPWVYCAQNYYAKWILDVKGDYCFLDIPGTSYVNLDKETTLEHINGCDVFIYMNWNSLNEPMDTLDELKERRPDLAPVLDSAKRVYTTKFSYSYDGNVNMDLLMEDFARMIHPECFDGGDSNLHYFKKLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>F6BC32</accession>
    <name>F6BC32_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0559</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96113.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96113">
        <property type="protein sequence ID" value="AEF96113"/>
        <property type="gene ID" value="Metig_0559"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0559"/>
    <dbReference type="HOGENOM" id="CLU_2645916_0_0_2"/>
    <dbReference type="OMA" id="PNTDNER"/>
    <dbReference type="OrthoDB" id="132063at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="23"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96113.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="76" mass="8838" checksum="BC1E78270905815C" modified="2011-07-27" version="1">MPFSYGPSMAVYPKAGYSGGDSMFTMILLILVIVLLVYLIVRKPNTDNERLVKMEKDIEEIKETVKELKRKWEEIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>F6BC47</accession>
    <name>F6BC47_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Extracellular ligand-binding receptor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0574</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96128.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798735.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0574"/>
    <dbReference type="EnsemblBacteria" id="AEF96128">
        <property type="protein sequence ID" value="AEF96128"/>
        <property type="gene ID" value="Metig_0574"/>
    </dbReference>
    <dbReference type="GeneID" id="10643412"/>
    <dbReference type="KEGG" id="mig:Metig_0574"/>
    <dbReference type="HOGENOM" id="CLU_027128_5_1_2"/>
    <dbReference type="OMA" id="KYIFRFV"/>
    <dbReference type="OrthoDB" id="23746at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028081">
        <property type="entry name" value="Leu-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13458">
        <property type="entry name" value="Peripla_BP_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="SSF53822"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0675">Receptor</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peripla_BP_6" evidence="1">
        <location>
            <begin position="36"/>
            <end position="406"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96128.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="424" mass="46858" checksum="B027CA384BA3409C" modified="2011-07-27" version="1">MWKKLLTLIFAVTIITAFSGCTNKTQETGTEASNIIKIGVLTDLSGDLSSDGKIVRNCIELAKEDVNNYFKEKGLPYQLEIYVEDTKCDPKLALEKLQVLKGKGVNVVIGPLSSSEVRNLASYATSNKIVIISPSSTAYPTFIGFAKPEDKKYIFRFVANDFFQSKAIKDEIKALGFKSVAMIYRGDAWGKGLHDATKENLEKEGIKVVEDIEYPTNPTPADWSPYIDKLEGAIRSAVDKHGKDKVAVVAIGFDELGSLLSQIEDDSILLQVTWVGSDGSAGSDKIIEQAKDKAAKVGLYSTLYHAESEEAEKIKEKYKAKFGGEVEQYGLIAYDATWVAGLAYAEVLKEKGSYDPDLMVEKIKEVLEKYNNGEFGVKPVTGDLKLNEWNDRASGDYAIYKVTEDGWKVVGIWKSSTGQVEWIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-06-02" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>F6BC54</accession>
    <name>F6BC54_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Conserved hypothetical cytosolic protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_0581</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96135.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798742.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96135">
        <property type="protein sequence ID" value="AEF96135"/>
        <property type="gene ID" value="Metig_0581"/>
    </dbReference>
    <dbReference type="GeneID" id="10643419"/>
    <dbReference type="KEGG" id="mig:Metig_0581"/>
    <dbReference type="HOGENOM" id="CLU_194452_0_0_2"/>
    <dbReference type="OMA" id="LPACYFT"/>
    <dbReference type="OrthoDB" id="117534at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF96135.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="68" mass="7995" checksum="6A84C630EEFD72A4" modified="2011-07-27" version="1">MECPNKEKNLNRCNCSYPSCSRKGLCCECLHYHLRNRQLPACCFPDDVEKTYDRSFEKFAELVMKGKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>F6BC78</accession>
    <name>F6BC78_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Putative archaetidylserine decarboxylase proenzyme</fullName>
            <ecNumber evidence="3">4.1.1.-</ecNumber>
        </recommendedName>
        <component>
            <recommendedName>
                <fullName evidence="3">Archaetidylserine decarboxylase alpha chain</fullName>
            </recommendedName>
        </component>
        <component>
            <recommendedName>
                <fullName evidence="3">Archaetidylserine decarboxylase beta chain</fullName>
            </recommendedName>
        </component>
    </protein>
    <gene>
        <name evidence="3" type="primary">asd</name>
        <name evidence="4" type="ordered locus">Metig_1752</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the formation of archaetidylethanolamine (PtdEtn) from archaetidylserine (PtdSer).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>archaetidylserine + H(+) = archaetidylethanolamine + CO2</text>
            <dbReference type="Rhea" id="RHEA:51488"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:71517"/>
            <dbReference type="ChEBI" id="CHEBI:134176"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>pyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
        </cofactor>
        <text evidence="3">Binds 1 pyruvoyl group covalently per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Heterodimer of a large membrane-associated beta subunit and a small pyruvoyl-containing alpha subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="3">Is synthesized initially as an inactive proenzyme. Formation of the active enzyme involves a self-maturation process in which the active site pyruvoyl group is generated from an internal serine residue via an autocatalytic post-translational modification. Two non-identical subunits are generated from the proenzyme in this reaction, and the pyruvate is formed at the N-terminus of the alpha chain, which is derived from the carboxyl end of the proenzyme. The post-translation cleavage follows an unusual pathway, termed non-hydrolytic serinolysis, in which the side chain hydroxyl group of the serine supplies its oxygen atom to form the C-terminus of the beta chain, while the remainder of the serine residue undergoes an oxidative deamination to produce ammonia and the pyruvoyl prosthetic group on the alpha chain.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the phosphatidylserine decarboxylase family. PSD-A subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.-" evidence="3"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97284.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799873.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1752"/>
    <dbReference type="EnsemblBacteria" id="AEF97284">
        <property type="protein sequence ID" value="AEF97284"/>
        <property type="gene ID" value="Metig_1752"/>
    </dbReference>
    <dbReference type="GeneID" id="10644626"/>
    <dbReference type="KEGG" id="mig:Metig_1752"/>
    <dbReference type="HOGENOM" id="CLU_072492_0_0_2"/>
    <dbReference type="OMA" id="VSIFMSP"/>
    <dbReference type="OrthoDB" id="105037at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004609">
        <property type="term" value="F:phosphatidylserine decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00664">
        <property type="entry name" value="PS_decarb_PSD_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003817">
        <property type="entry name" value="PS_Dcarbxylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033175">
        <property type="entry name" value="PSD-A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35809">
        <property type="entry name" value="PTHR35809"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02666">
        <property type="entry name" value="PS_Dcarbxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00164">
        <property type="entry name" value="PS_decarb_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="1 3" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="3" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="3" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="2 3" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="3" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="3" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0865">Zymogen</keyword>
    <feature type="chain" id="PRO_5029076652" description="Archaetidylserine decarboxylase beta chain" evidence="3">
        <location>
            <begin position="1"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5029076651" description="Archaetidylserine decarboxylase alpha chain" evidence="3">
        <location>
            <begin position="172"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with substrate; via pyruvic acid" evidence="3">
        <location>
            <position position="172"/>
        </location>
    </feature>
    <feature type="site" description="Cleavage (non-hydrolytic); by autocatalysis" evidence="3">
        <location>
            <begin position="171"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="modified residue" description="Pyruvic acid (Ser); by autocatalysis" evidence="3">
        <location>
            <position position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEF97284.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="203" mass="22729" checksum="A7A6DAE5EA9AE2E7" modified="2011-07-27" version="1">MKRYHKFIAVGVCSLLLFTVYFYRDPERTIPNEKGIIVSPADGTVLYIKSYNGVPEVYKDGNSYILKDIQKYYPNGSYVIGIFMSPFDVHVNRAPISGKVVYVKHFDGALYPAFGEGVITKNERNIIIIKNNNTYIGVVQIAGTMARRTVCNVKEGDYVQIGERIGMIKLGSQTAVIIPKTYNITVKKGDKVYAGESIIARQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>F6BC93</accession>
    <name>F6BC93_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_1767</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97299.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1767"/>
    <dbReference type="EnsemblBacteria" id="AEF97299">
        <property type="protein sequence ID" value="AEF97299"/>
        <property type="gene ID" value="Metig_1767"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_1767"/>
    <dbReference type="HOGENOM" id="CLU_3371348_0_0_2"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF97299.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="34" mass="3858" checksum="660B6CA2A0BBFE1A" modified="2011-07-27" version="1">MNEEIKKVFVIGLDSAPPELLFGRFLDELPNLLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCB0</accession>
    <name>F6BCB0_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Type II site-specific deoxyribonuclease</fullName>
            <ecNumber evidence="2">3.1.21.4</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1784</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.1.21.4" evidence="2"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97316.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="REBASE" id="36264">
        <property type="entry name" value="Mig5ORF1783P"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF97316">
        <property type="protein sequence ID" value="AEF97316"/>
        <property type="gene ID" value="Metig_1784"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_1784"/>
    <dbReference type="HOGENOM" id="CLU_095647_0_0_2"/>
    <dbReference type="OMA" id="AKCINGE"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009036">
        <property type="term" value="F:type II site-specific deoxyribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041551">
        <property type="entry name" value="RE_BsaWI"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18643">
        <property type="entry name" value="RE_BsaWI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RE_BsaWI" evidence="1">
        <location>
            <begin position="83"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF18643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF97316.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="218" mass="25720" checksum="91178C89827131B1" modified="2011-07-27" version="1">MEINNISKILEIEREEYIKNKVKEYIKQGFSKSEAINKANQSWRAYIGSKIQDIIYKILEDLLKDTELKLTTDKILASKNLTEELDKVKRLIAVNYGEYLFLPDADIIVYKVKDNEVKIIAIISVKNSFRERGFETTYWKLKLKESPVTSHIKVFLATPDKDNEISYKCPNGRPRKMRIILEYELDGIYFLKDDFEETEKVKHFEKIVEDILRISNEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCC0</accession>
    <name>F6BCC0_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Coenzyme F420-reducing hydrogenase delta subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_1794</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97326.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799915.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1794"/>
    <dbReference type="EnsemblBacteria" id="AEF97326">
        <property type="protein sequence ID" value="AEF97326"/>
        <property type="gene ID" value="Metig_1794"/>
    </dbReference>
    <dbReference type="GeneID" id="10644671"/>
    <dbReference type="KEGG" id="mig:Metig_1794"/>
    <dbReference type="HOGENOM" id="CLU_099037_0_2_2"/>
    <dbReference type="OMA" id="CQKKYVS"/>
    <dbReference type="OrthoDB" id="122958at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008047">
        <property type="term" value="F:enzyme activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06064">
        <property type="entry name" value="H2MP_F420-Reduc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004411">
        <property type="entry name" value="Pept_A31_F420-red_hyd_d"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023430">
        <property type="entry name" value="Pept_HybD-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000671">
        <property type="entry name" value="Peptidase_A31"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30302">
        <property type="entry name" value="PTHR30302"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01750">
        <property type="entry name" value="HycI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00446">
        <property type="entry name" value="HYDRGNUPTAKE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53163">
        <property type="entry name" value="SSF53163"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00130">
        <property type="entry name" value="frhD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00072">
        <property type="entry name" value="hydrog_prot"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF97326.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="181" mass="20340" checksum="5611CD5652BB8D3B" modified="2011-07-27" version="1">MKTRLDIEDEDLFDFTPSYLKKEIMVLACGNILFADDGFSVHVIEKLNNILTEEEKKNIALVDAGAGAPQQVLTLIDENSKTKKIIVVDVIDFGLKPGEIKILKKDDLPNPEHNRIDIHDWPLSTILRQVSDTFGIEIMVVGCQAKYISEPDVVIGLSKEVEEAVDKAVEIILKELRKNQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCC3</accession>
    <name>F6BCC3_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Dolichyl-phosphooligosaccharide-protein glycotransferase</fullName>
            <ecNumber evidence="6">2.4.99.21</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Oligosaccharyl transferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ordered locus">Metig_1797</name>
    </gene>
    <organism evidence="18">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the STT3 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.99.21" evidence="6"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97329.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799918.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1797"/>
    <dbReference type="EnsemblBacteria" id="AEF97329">
        <property type="protein sequence ID" value="AEF97329"/>
        <property type="gene ID" value="Metig_1797"/>
    </dbReference>
    <dbReference type="GeneID" id="10644674"/>
    <dbReference type="KEGG" id="mig:Metig_1797"/>
    <dbReference type="HOGENOM" id="CLU_018380_0_0_2"/>
    <dbReference type="OMA" id="WDNGHIY"/>
    <dbReference type="OrthoDB" id="2200at2157"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004576">
        <property type="term" value="F:oligosaccharyl transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006486">
        <property type="term" value="P:protein glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003674">
        <property type="entry name" value="Oligo_trans_STT3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13872">
        <property type="entry name" value="PTHR13872"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02516">
        <property type="entry name" value="STT3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="14" id="KW-0464">Manganese</keyword>
    <keyword evidence="13 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 17" id="KW-0808">Transferase</keyword>
    <keyword evidence="9 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="139"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="166"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="190"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="221"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="242"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="278"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="348"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="375"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="400"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="430"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="462"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="501"/>
            <end position="521"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00030679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="AEF97329.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="853" mass="95721" checksum="56AC9FFB843BA833" modified="2011-07-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCD1</accession>
    <name>F6BCD1_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Hydrogenase maturation factor HypA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">hypA</name>
        <name evidence="6" type="ordered locus">Metig_0588</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Involved in the maturation of [NiFe] hydrogenases. Required for nickel insertion into the metal center of the hydrogenase.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the HypA/HybF family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96142.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798749.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0588"/>
    <dbReference type="EnsemblBacteria" id="AEF96142">
        <property type="protein sequence ID" value="AEF96142"/>
        <property type="gene ID" value="Metig_0588"/>
    </dbReference>
    <dbReference type="GeneID" id="10643426"/>
    <dbReference type="KEGG" id="mig:Metig_0588"/>
    <dbReference type="HOGENOM" id="CLU_126929_2_1_2"/>
    <dbReference type="OMA" id="ILLCPCG"/>
    <dbReference type="OrthoDB" id="109617at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016151">
        <property type="term" value="F:nickel cation binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051604">
        <property type="term" value="P:protein maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00213">
        <property type="entry name" value="HypA_HybF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020538">
        <property type="entry name" value="Hydgase_Ni_incorp_HypA/HybF_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000688">
        <property type="entry name" value="HypA/HybF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34535">
        <property type="entry name" value="PTHR34535"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01155">
        <property type="entry name" value="HypA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004761">
        <property type="entry name" value="Hydrgn_mat_HypA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00100">
        <property type="entry name" value="hypA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01249">
        <property type="entry name" value="HYPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 5" id="KW-0533">Nickel</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0862">Zinc</keyword>
    <feature type="binding site" evidence="5">
        <location>
            <position position="2"/>
        </location>
        <ligand>
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="78"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="81"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="97"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="100"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEF96142.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="124" mass="14063" checksum="A2AB9E5D7D2BE841" modified="2011-07-27" version="1">MHELSYATSMLDTIINAVKEQEKDGKKIKKVSEINLEVGELTFINIEQLKFAFEVIAEGTICENAKINVQFIKPLAVCKDCGYEGNVKAEDEFEIYCPNCNSMNLKFKGGKEFNIKNIVVEFDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCD7</accession>
    <name>F6BCD7_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DNA-binding protein Metig_0594</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metig_0594</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the PDCD5 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96148.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798755.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0594"/>
    <dbReference type="EnsemblBacteria" id="AEF96148">
        <property type="protein sequence ID" value="AEF96148"/>
        <property type="gene ID" value="Metig_0594"/>
    </dbReference>
    <dbReference type="GeneID" id="10643432"/>
    <dbReference type="KEGG" id="mig:Metig_0594"/>
    <dbReference type="HOGENOM" id="CLU_122978_3_0_2"/>
    <dbReference type="OMA" id="KPQFGEQ"/>
    <dbReference type="OrthoDB" id="130316at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00026">
        <property type="entry name" value="dsDNA_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022889">
        <property type="entry name" value="DNA_bind_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002836">
        <property type="entry name" value="PDCD5-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036883">
        <property type="entry name" value="PDCD5-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10840">
        <property type="entry name" value="PTHR10840"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01984">
        <property type="entry name" value="dsDNA_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015730">
        <property type="entry name" value="TFAR19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46950">
        <property type="entry name" value="SSF46950"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2 4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEF96148.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="112" mass="13660" checksum="6BBEC45267229F50" modified="2011-07-27" version="1">MDLEEIKRRKLLEMQKKLEEQEKLQQLQEQQMQYQLQKQKILRQILTEEARSRLARIRMAKPEFAEQVELQLIQLAQMGRLPIPVTDEQLKLLLDRIHEATKKKREFKIIRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-06-02" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCF9</accession>
    <name>F6BCF9_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_0620</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96170.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96170">
        <property type="protein sequence ID" value="AEF96170"/>
        <property type="gene ID" value="Metig_0620"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0620"/>
    <dbReference type="HOGENOM" id="CLU_1340754_0_0_2"/>
    <dbReference type="OMA" id="QFYPHNI"/>
    <dbReference type="OrthoDB" id="90998at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF96170.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="203" mass="23156" checksum="8F3AB9A8BA768589" modified="2011-07-27" version="1">MMKKFIIFGIMCLLVSSCLCIEQRNNYKISNDDLLKYSKTLKTEINGVSVEIPLRCTINDAMKTKLVNTSDKEIKEYYLTKPIIYLEYNLSLSQKDGGVSIMDLVGKLIWLNNFYPHKMVIPINVSENITAKVILNNKEGYTTFTPSEIEMIKNSNKTMIIKVIKTNNNATITKIKNTFIIEGNSLYALDMAESRFIIAICNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-06-02" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCI4</accession>
    <name>F6BCI4_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Holliday junction resolvase-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0645</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96195.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798799.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96195">
        <property type="protein sequence ID" value="AEF96195"/>
        <property type="gene ID" value="Metig_0645"/>
    </dbReference>
    <dbReference type="GeneID" id="10643485"/>
    <dbReference type="KEGG" id="mig:Metig_0645"/>
    <dbReference type="HOGENOM" id="CLU_105716_1_1_2"/>
    <dbReference type="OMA" id="MVRWELI"/>
    <dbReference type="OrthoDB" id="117035at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019287">
        <property type="entry name" value="Hday_junct_resolvase-rel_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10107">
        <property type="entry name" value="Endonuc_Holl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Endonuc_Holl" evidence="1">
        <location>
            <begin position="1"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF10107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96195.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="125" mass="14779" checksum="A75261519504B6C2" modified="2011-07-27" version="1">MEQRARQLFEEWKDKELEKLSKQKADMLFEDWKRKYEKKIRKDAIEKSKSVIIGKVTEHFAPFLPEFKYNPKDARFIGSPVDFIVFDGLDEGDLKKIVFLDVKTGKSPLSKREKLIKKIVESKKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-09-29" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCI5</accession>
    <name>F6BCI5_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_0646</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96196.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798800.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96196">
        <property type="protein sequence ID" value="AEF96196"/>
        <property type="gene ID" value="Metig_0646"/>
    </dbReference>
    <dbReference type="GeneID" id="10643486"/>
    <dbReference type="KEGG" id="mig:Metig_0646"/>
    <dbReference type="HOGENOM" id="CLU_2340193_0_0_2"/>
    <dbReference type="OMA" id="VGEIGEC"/>
    <dbReference type="OrthoDB" id="104553at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF96196.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="97" mass="11330" checksum="9E886955FE5BBD4A" modified="2011-07-27" version="1">MECPYCGGDCVNKSAVEIYLDMVEMFFKYKDEDSEVTYRKYPTVGEVGECKKTHGRIWLCPYCKKPFKENYKLKEVTIQCPNCKKTLCIPATNRTFC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCI7</accession>
    <name>F6BCI7_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Radical SAM domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0648</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96198.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798801.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96198">
        <property type="protein sequence ID" value="AEF96198"/>
        <property type="gene ID" value="Metig_0648"/>
    </dbReference>
    <dbReference type="GeneID" id="10643488"/>
    <dbReference type="KEGG" id="mig:Metig_0648"/>
    <dbReference type="HOGENOM" id="CLU_011543_3_3_2"/>
    <dbReference type="OMA" id="PESYNWS"/>
    <dbReference type="OrthoDB" id="5945at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.30.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaB/NifB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045784">
        <property type="entry name" value="Radical_SAM_N2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023404">
        <property type="entry name" value="rSAM_horseshoe"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19864">
        <property type="entry name" value="Radical_SAM_N2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Radical SAM core" evidence="1">
        <location>
            <begin position="164"/>
            <end position="381"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96198.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="444" mass="51024" checksum="4E58737701EFA3A8" modified="2011-07-27" version="1">MKNIKNVAVIFPNKFKGGITCLAMHILHYHLNKYRDIDCKMFFIENYHEIRNYDAIIITLQYENDYFNAIKIIKELKPKNPNAIFIGGGPCAISNPFPLSRFFDAFVIGEIEGTEVMYNLITKNFDEIKEGVYFPDKEGKIKRIYPKKLTIDDYPVNQITHESGAYGKAFLLEIGRGCPRRCRFCMARSIYYPPRFRKLDDLIYLTDKGLEYTKVNKVALIAPSVGDYKYIVDLCQYLNERGVQISPSSLRADTITDELLSLLNLKTLTIAPEAGSERLREVIKKDINEDDILNAVALAKEHGIDKIKLYFMVGIVDERDEDVEEIINLTKKIKENIRKVEVSINPMVPKPRTPFEKESFDLTAKNKIKYIEKNLKKIGVSVESENFNSMIAQCILCRGGEELGNILENSEKPIRLIKNIKNAGLLDKYLGKIEGELPWERIEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCL3</accession>
    <name>F6BCL3_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Ferredoxin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0674</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96224.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0674"/>
    <dbReference type="EnsemblBacteria" id="AEF96224">
        <property type="protein sequence ID" value="AEF96224"/>
        <property type="gene ID" value="Metig_0674"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0674"/>
    <dbReference type="HOGENOM" id="CLU_019091_0_0_2"/>
    <dbReference type="OMA" id="NPVMHHL"/>
    <dbReference type="OrthoDB" id="10685at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00207">
        <property type="entry name" value="fer2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.480">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036010">
        <property type="entry name" value="2Fe-2S_ferredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001041">
        <property type="entry name" value="2Fe-2S_ferredoxin-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027980">
        <property type="entry name" value="RACo_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041414">
        <property type="entry name" value="Raco_middle"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042259">
        <property type="entry name" value="Raco_middle_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00111">
        <property type="entry name" value="Fer2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14574">
        <property type="entry name" value="RACo_C_ter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17651">
        <property type="entry name" value="Raco_middle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54292">
        <property type="entry name" value="SSF54292"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51085">
        <property type="entry name" value="2FE2S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="2Fe-2S ferredoxin-type" evidence="1">
        <location>
            <begin position="3"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96224.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="553" mass="60025" checksum="84918AB67ADF7C7E" modified="2011-07-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCP3</accession>
    <name>F6BCP3_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Acetolactate synthase small subunit</fullName>
            <shortName evidence="6">AHAS</shortName>
            <shortName evidence="6">ALS</shortName>
            <ecNumber evidence="6">2.2.1.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Acetohydroxy-acid synthase small subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Metig_0704</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes the conversion of 2 pyruvate molecules into acetolactate in the first common step of the biosynthetic pathway of the branched-amino acids such as leucine, isoleucine, and valine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>H(+) + 2 pyruvate = (2S)-2-acetolactate + CO2</text>
            <dbReference type="Rhea" id="RHEA:25249"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:58476"/>
            <dbReference type="EC" id="2.2.1.6"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 6">Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 1/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 6">Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 1/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Dimer of large and small chains.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 6">Belongs to the acetolactate synthase small subunit family.</text>
    </comment>
    <dbReference type="EC" id="2.2.1.6" evidence="6"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96254.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798857.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0704"/>
    <dbReference type="EnsemblBacteria" id="AEF96254">
        <property type="protein sequence ID" value="AEF96254"/>
        <property type="gene ID" value="Metig_0704"/>
    </dbReference>
    <dbReference type="GeneID" id="10643544"/>
    <dbReference type="KEGG" id="mig:Metig_0704"/>
    <dbReference type="HOGENOM" id="CLU_055003_1_3_2"/>
    <dbReference type="OMA" id="EPYGIKE"/>
    <dbReference type="OrthoDB" id="85263at2157"/>
    <dbReference type="UniPathway" id="UPA00047">
        <property type="reaction ID" value="UER00055"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00049">
        <property type="reaction ID" value="UER00059"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003984">
        <property type="term" value="F:acetolactate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990610">
        <property type="term" value="F:acetolactate synthase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009097">
        <property type="term" value="P:isoleucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009099">
        <property type="term" value="P:valine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04878">
        <property type="entry name" value="ACT_AHAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004789">
        <property type="entry name" value="Acetalactate_synth_ssu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027271">
        <property type="entry name" value="Acetolactate_synth/TF_NikR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019455">
        <property type="entry name" value="Acetolactate_synth_ssu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002912">
        <property type="entry name" value="ACT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039557">
        <property type="entry name" value="AHAS_ACT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30239">
        <property type="entry name" value="PTHR30239"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10369">
        <property type="entry name" value="ALS_ss_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="SSF55021"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00119">
        <property type="entry name" value="acolac_sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51671">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="5 6" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="ACT" evidence="7">
        <location>
            <begin position="6"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006341"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU368092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEF96254.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="168" mass="18758" checksum="44616A859A29F957" modified="2011-07-27" version="1">MEHKHVISALVLHKPGVLQRISGLFTRRGFNIASITVGITENPEIARMTIVVKGDDKVLEQVIKQLNKLIDVIKVSDMDEKKTVQRELCLIKVYAPTESAKSQVIQYTNIFRGKIVDLSAETLTVEITGDEDKINAFIDLVRPLGIKEMARTGPTALMRGPKILKPKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCQ0</accession>
    <name>F6BCQ0_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 12">Biotin synthase</fullName>
            <ecNumber evidence="3 12">2.8.1.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">bioB</name>
        <name evidence="15" type="ordered locus">Metig_0711</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>(4R,5S)-dethiobiotin + [sulfur carrier]-SH + 2 reduced [2Fe-2S]-[ferredoxin] + 2 S-adenosyl-L-methionine = 2 5'-deoxyadenosine + [sulfur carrier]-H + biotin + 2 L-methionine + 2 oxidized [2Fe-2S]-[ferredoxin]</text>
            <dbReference type="Rhea" id="RHEA:22060"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="Rhea" id="RHEA-COMP:14737"/>
            <dbReference type="Rhea" id="RHEA-COMP:14739"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:29917"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:57586"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:64428"/>
            <dbReference type="ChEBI" id="CHEBI:149473"/>
            <dbReference type="EC" id="2.8.1.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12 13">
            <name>2Fe-2S cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49601"/>
        </cofactor>
        <text evidence="12 13">Binds 1 [2Fe-2S] cluster. The cluster is coordinated with 3 cysteines and 1 arginine.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12 13">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="12 13">Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 12">Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the radical SAM superfamily. Biotin synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.8.1.6" evidence="3 12"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96261.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798864.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0711"/>
    <dbReference type="EnsemblBacteria" id="AEF96261">
        <property type="protein sequence ID" value="AEF96261"/>
        <property type="gene ID" value="Metig_0711"/>
    </dbReference>
    <dbReference type="GeneID" id="10643551"/>
    <dbReference type="KEGG" id="mig:Metig_0711"/>
    <dbReference type="HOGENOM" id="CLU_033172_2_1_2"/>
    <dbReference type="OMA" id="ADRFCMG"/>
    <dbReference type="OrthoDB" id="55121at2157"/>
    <dbReference type="UniPathway" id="UPA00078">
        <property type="reaction ID" value="UER00162"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004076">
        <property type="term" value="F:biotin synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009102">
        <property type="term" value="P:biotin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01694">
        <property type="entry name" value="BioB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010722">
        <property type="entry name" value="BATS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002684">
        <property type="entry name" value="Biotin_synth/BioAB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024177">
        <property type="entry name" value="Biotin_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaB/NifB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22976">
        <property type="entry name" value="PTHR22976"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06968">
        <property type="entry name" value="BATS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001619">
        <property type="entry name" value="Biotin_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00876">
        <property type="entry name" value="BATS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00433">
        <property type="entry name" value="bioB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 12 13" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="4 12 13" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="9 12" id="KW-0093">Biotin biosynthesis</keyword>
    <keyword evidence="10 12 13" id="KW-0408">Iron</keyword>
    <keyword evidence="11 12 13" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="8 12 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 12 13" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5 12 15" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Radical SAM core" evidence="14">
        <location>
            <begin position="43"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12 13">
        <location>
            <position position="61"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12 13">
        <location>
            <position position="65"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12 13">
        <location>
            <position position="68"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12 13">
        <location>
            <position position="104"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12 13">
        <location>
            <position position="136"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12 13">
        <location>
            <position position="195"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12 13">
        <location>
            <position position="271"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR001619-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="AEF96261.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="321" mass="36973" checksum="964F08F9D9C96F08" modified="2011-07-27" version="1">MEIFKFYEKSINNKIDFEDALELYENFDAYDLLYLAYKIKKAFKKNKIDLCSIINAKSGKCSENCAFCSQSIYHKTDIQVYPLKSKEEILNKAKKMEKCCNRFSIVVSGKSISDEEFEKVVEIIEEIKKQTKLKVCTSLGIIDKDKLKALKELDVRYHHNLESSRNHFKNICTTHTYEDRVKTIKKAKKLGLEVCSGGIFGLGEDFKERIDMMMDLKKLDVDSVALNILHPIKGTKMCDLIEKNEIKPISPMEALKSIAICRVIMPDKEIRLCGGREFNLHDLQCLSLLALDGLMVGDYLTTKGRVLEDDLRMIRDMGFIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCR0</accession>
    <name>F6BCR0_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ribosomal RNA small subunit methyltransferase Nep1</fullName>
            <ecNumber evidence="5">2.1.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">16S rRNA (pseudouridine-N1-)-methyltransferase Nep1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">nep1</name>
        <name evidence="6" type="ordered locus">Metig_0721</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Methyltransferase involved in ribosomal biogenesis. Specifically catalyzes the N1-methylation of the pseudouridine corresponding to position 914 in M.jannaschii 16S rRNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>a pseudouridine in 16S/18S rRNA + S-adenosyl-L-methionine = an N(1)-methylpseudouridine in 16S/18S rRNA + H(+) + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:46696"/>
            <dbReference type="Rhea" id="RHEA-COMP:11633"/>
            <dbReference type="Rhea" id="RHEA-COMP:11634"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:74890"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the class IV-like SAM-binding methyltransferase superfamily. RNA methyltransferase NEP1 family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="5"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96271.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798874.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0721"/>
    <dbReference type="EnsemblBacteria" id="AEF96271">
        <property type="protein sequence ID" value="AEF96271"/>
        <property type="gene ID" value="Metig_0721"/>
    </dbReference>
    <dbReference type="GeneID" id="10643561"/>
    <dbReference type="KEGG" id="mig:Metig_0721"/>
    <dbReference type="HOGENOM" id="CLU_055846_1_3_2"/>
    <dbReference type="OMA" id="VYVHTRN"/>
    <dbReference type="OrthoDB" id="72065at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070037">
        <property type="term" value="F:rRNA (pseudouridine) methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070475">
        <property type="term" value="P:rRNA base methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18088">
        <property type="entry name" value="Nep1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1280.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00554">
        <property type="entry name" value="NEP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029028">
        <property type="entry name" value="Alpha/beta_knot_MTases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005304">
        <property type="entry name" value="Rbsml_bgen_MeTrfase_EMG1/NEP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023503">
        <property type="entry name" value="Ribosome_NEP1_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029026">
        <property type="entry name" value="tRNA_m1G_MTases_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12636">
        <property type="entry name" value="PTHR12636"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03587">
        <property type="entry name" value="EMG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75217">
        <property type="entry name" value="SSF75217"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="4 5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="3 5" id="KW-0699">rRNA-binding</keyword>
    <keyword evidence="5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="S-adenosyl-L-methionine binding" evidence="5">
        <location>
            <begin position="181"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="162"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="167"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="site" description="Interaction with substrate rRNA" evidence="5">
        <location>
            <position position="54"/>
        </location>
    </feature>
    <feature type="site" description="Stabilizes Arg-xx" evidence="5">
        <location>
            <position position="56"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with substrate rRNA" evidence="5">
        <location>
            <position position="95"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with substrate rRNA" evidence="5">
        <location>
            <position position="98"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with substrate rRNA" evidence="5">
        <location>
            <position position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEF96271.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="201" mass="23395" checksum="7FC1988EDD312601" modified="2011-07-27" version="1">MKNIILAKSALELIPKEIKGKVKKRKSLFKYDILDSNYHYKFMRDLKDWEMRGRPDIVHFSLLTILDSPICREGKLNVYVHTYDNKVIYINPKTRLPRNYFRFLGVMEKLLKGNPNPLMRVEEKTLEELLHEINAKRVILFSKEGKLSHPSVVKGYDTFIIGGFPKGTLNVDFENVDKISIYPEGLMAWTVCGICCSFLSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCS0</accession>
    <name>F6BCS0_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Putative signal transduction protein with CBS domains</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Metig_0731</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96281.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798884.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0731"/>
    <dbReference type="EnsemblBacteria" id="AEF96281">
        <property type="protein sequence ID" value="AEF96281"/>
        <property type="gene ID" value="Metig_0731"/>
    </dbReference>
    <dbReference type="GeneID" id="10643571"/>
    <dbReference type="KEGG" id="mig:Metig_0731"/>
    <dbReference type="HOGENOM" id="CLU_040681_7_0_2"/>
    <dbReference type="OMA" id="APIKVYM"/>
    <dbReference type="OrthoDB" id="83765at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044065">
        <property type="entry name" value="ACP_MB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="SSF54631"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51901">
        <property type="entry name" value="ACP_MB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0129">CBS domain</keyword>
    <keyword evidence="2" id="KW-0408">Iron</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="CBS" evidence="3">
        <location>
            <begin position="7"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="3">
        <location>
            <begin position="73"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="domain" description="ACP-type MB" evidence="4">
        <location>
            <begin position="146"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="151"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="154"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="169"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51901"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEF96281.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="176" mass="19367" checksum="CFCFC003658CE05D" modified="2011-07-27" version="1">MVVSDAMSTPVITVDTNTTVYDVANIMKEKKIGCVVVVEKDSKPLGIVTERDLVINIVAKNLKPKDVLVKDIMTTKLITISPNATLMDAARKMAEKNIKRLPVVEGDKLLGIITVSDITKISPKIFEIMYEMSKIGGELPYRGDEYIEGICEVCGSQGSVRLVNGRYICDNCIDEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCS9</accession>
    <name>F6BCS9_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0216 protein Metig_0742</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0742</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0216 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96290.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798893.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0742"/>
    <dbReference type="EnsemblBacteria" id="AEF96290">
        <property type="protein sequence ID" value="AEF96290"/>
        <property type="gene ID" value="Metig_0742"/>
    </dbReference>
    <dbReference type="GeneID" id="10643581"/>
    <dbReference type="KEGG" id="mig:Metig_0742"/>
    <dbReference type="HOGENOM" id="CLU_146474_1_0_2"/>
    <dbReference type="OMA" id="DNTFFIM"/>
    <dbReference type="OrthoDB" id="112654at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00585">
        <property type="entry name" value="UPF0216"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002746">
        <property type="entry name" value="UPF0216"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01886">
        <property type="entry name" value="DUF61"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005264">
        <property type="entry name" value="UCP005264"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96290.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="129" mass="15148" checksum="46C3C379AB8F91EE" modified="2011-07-27" version="1">MDKEIYRLIYDLSPRFKRKTLGELLNEDKPYIIVNGKRHRIKKRELLKLKEIASEDLEIPIVLEVDASLESGTIKVSGKEAVKVVSKILGREYNPFSEEDVLYIYKPELRILRRELPTTTQLLFRLSMD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCT3</accession>
    <name>F6BCT3_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Site-specific DNA-methyltransferase (adenine-specific)</fullName>
            <ecNumber evidence="2">2.1.1.72</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metig_0746</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 2'-deoxyadenosine in DNA + S-adenosyl-L-methionine = an N(6)-methyl-2'-deoxyadenosine in DNA + H(+) + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:15197"/>
            <dbReference type="Rhea" id="RHEA-COMP:12418"/>
            <dbReference type="Rhea" id="RHEA-COMP:12419"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:90615"/>
            <dbReference type="ChEBI" id="CHEBI:90616"/>
            <dbReference type="EC" id="2.1.1.72"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.1.1.72" evidence="2"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96294.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798897.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0746"/>
    <dbReference type="REBASE" id="36258">
        <property type="entry name" value="M.Mig5ORF746P"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96294">
        <property type="protein sequence ID" value="AEF96294"/>
        <property type="gene ID" value="Metig_0746"/>
    </dbReference>
    <dbReference type="GeneID" id="10643585"/>
    <dbReference type="KEGG" id="mig:Metig_0746"/>
    <dbReference type="HOGENOM" id="CLU_013049_4_2_2"/>
    <dbReference type="OMA" id="NSADWAW"/>
    <dbReference type="OrthoDB" id="45297at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008170">
        <property type="term" value="F:N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009007">
        <property type="term" value="F:site-specific DNA-methyltransferase (adenine-specific) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1260.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003356">
        <property type="entry name" value="DNA_methylase_A-5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002052">
        <property type="entry name" value="DNA_methylase_N6_adenine_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038333">
        <property type="entry name" value="T1MK-like_N_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02384">
        <property type="entry name" value="N6_Mtase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00092">
        <property type="entry name" value="N6_MTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N6_Mtase" evidence="3">
        <location>
            <begin position="226"/>
            <end position="541"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEF96294.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="576" mass="66455" checksum="878B424A32EE327C" modified="2011-07-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-06-02" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCU0</accession>
    <name>F6BCU0_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">DNA polymerase beta domain protein region</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0754</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96301.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798904.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96301">
        <property type="protein sequence ID" value="AEF96301"/>
        <property type="gene ID" value="Metig_0754"/>
    </dbReference>
    <dbReference type="GeneID" id="10643593"/>
    <dbReference type="KEGG" id="mig:Metig_0754"/>
    <dbReference type="HOGENOM" id="CLU_130257_3_2_2"/>
    <dbReference type="OMA" id="INWSFHR"/>
    <dbReference type="OrthoDB" id="108083at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002934">
        <property type="entry name" value="Polymerase_NTP_transf_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01909">
        <property type="entry name" value="NTP_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="SSF81301"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NTP_transf_2" evidence="1">
        <location>
            <begin position="7"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96301.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="100" mass="11611" checksum="A24BED977D4A9652" modified="2011-07-27" version="1">MNEEKAIKEFVNALKSKYKGRIKKIILFGSYARGDYTEESDIDILIVGDVDFDYVIDLCTKLLLKYGVVINAIIESEEVFNKKINWSFHRNVLEEGRALY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCX3</accession>
    <name>F6BCX3_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_0789</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96334.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0789"/>
    <dbReference type="EnsemblBacteria" id="AEF96334">
        <property type="protein sequence ID" value="AEF96334"/>
        <property type="gene ID" value="Metig_0789"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0789"/>
    <dbReference type="HOGENOM" id="CLU_161126_0_0_2"/>
    <dbReference type="OMA" id="FNIQATS"/>
    <dbReference type="OrthoDB" id="113697at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019270">
        <property type="entry name" value="DUF2283"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10049">
        <property type="entry name" value="DUF2283"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF96334.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="121" mass="13704" checksum="117FBBDD65A1E4F5" modified="2011-07-27" version="1">MIINNKATNIDYDIENDSLYIYAEGQEYKDSLDLNDIIIDFNKEGSVMGIEVLDASKKFKLSKFDLKNIVEFKSTIFISKDIVKIEMKISILKRNRMVEKSVIAKGLNDVSLSEGTLAISC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>F6BD07</accession>
    <name>F6BD07_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Proteasome-activating nucleotidase</fullName>
            <shortName evidence="5">PAN</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Proteasomal ATPase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">Proteasome regulatory ATPase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">Proteasome regulatory particle</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">pan</name>
        <name evidence="8" type="ordered locus">Metig_0823</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">ATPase which is responsible for recognizing, binding, unfolding and translocation of substrate proteins into the archaeal 20S proteasome core particle. Is essential for opening the gate of the 20S proteasome via an interaction with its C-terminus, thereby allowing substrate entry and access to the site of proteolysis. Thus, the C-termini of the proteasomal ATPase function like a 'key in a lock' to induce gate opening and therefore regulate proteolysis. Unfolding activity requires energy from ATP hydrolysis, whereas ATP binding alone promotes ATPase-20S proteasome association which triggers gate opening, and supports translocation of unfolded substrates.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homohexamer. The hexameric complex has a two-ring architecture resembling a top hat that caps the 20S proteasome core at one or both ends. Upon ATP-binding, the C-terminus of PAN interacts with the alpha-rings of the proteasome core by binding to the intersubunit pockets.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="5">Consists of three main regions, an N-terminal coiled-coil domain that may assist in substrate recognition, an interdomain involved in PAN hexamerization, and a C-terminal ATPase domain of the AAA type.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5 6">Belongs to the AAA ATPase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96368.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798970.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0823"/>
    <dbReference type="EnsemblBacteria" id="AEF96368">
        <property type="protein sequence ID" value="AEF96368"/>
        <property type="gene ID" value="Metig_0823"/>
    </dbReference>
    <dbReference type="GeneID" id="10643664"/>
    <dbReference type="KEGG" id="mig:Metig_0823"/>
    <dbReference type="HOGENOM" id="CLU_000688_2_2_2"/>
    <dbReference type="OMA" id="YNMTTFE"/>
    <dbReference type="OrthoDB" id="30571at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022623">
        <property type="term" value="C:proteasome-activating nucleotidase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036402">
        <property type="term" value="F:proteasome-activating activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010498">
        <property type="term" value="P:proteasomal protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043335">
        <property type="term" value="P:protein unfolding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00553">
        <property type="entry name" value="PAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005937">
        <property type="entry name" value="26S_Psome_P45-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023501">
        <property type="entry name" value="Nucleotidase_PAN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032501">
        <property type="entry name" value="Prot_ATP_ID_OB_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16450">
        <property type="entry name" value="Prot_ATP_ID_OB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01242">
        <property type="entry name" value="26Sp45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 5" id="KW-0143">Chaperone</keyword>
    <keyword evidence="3 5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2 5 8" id="KW-0647">Proteasome</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA" evidence="7">
        <location>
            <begin position="181"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="27"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="192"/>
            <end position="197"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="331"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEF96368.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="407" mass="45771" checksum="867F0C2B32DCACCA" modified="2011-07-27" version="1">MTYEEYNDIKENNEKMDLNEFKEKAYIAELESKLLRMEMEKRDLERENLQLIKENEILKRELDRLRVPPLIIGTVIEKVSDRKVIVKSSTGPHFLVNVSQFVNPDDLTPGTRVCLNQQTLSVVDVLPKEKDLRAMAMEVDERPKVSFSDIGGLAKQIQEIKEVVELPLKHPELFEKVGIEPPKGVLLYGPPGTGKTLLAKAVAAETNATFIKIVGSELVKKFIGEGAKLVRDVFKLAKEKAPSIIFIDEIDAVAAKRTEALTGGDREVQRTLMQLLAEMDGFDARGDVKVIGATNRLDILDPAILRPGRFDRIIEIPAPNEEGRLEILKIHTRKMNLRDVDLKRIAKMTEGCVGAELKAICTEAGMFAIRDGRDYVTMDDFLKAVDKVMGKKSRGEVISPHLEVLYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>F6BD09</accession>
    <name>F6BD09_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Anthranilate phosphoribosyltransferase</fullName>
            <ecNumber evidence="3">2.4.2.18</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">trpD</name>
        <name evidence="6" type="ordered locus">Metig_0825</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the transfer of the phosphoribosyl group of 5-phosphorylribose-1-pyrophosphate (PRPP) to anthranilate to yield N-(5'-phosphoribosyl)-anthranilate (PRA).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>diphosphate + N-(5-phospho-beta-D-ribosyl)anthranilate = 5-phospho-alpha-D-ribose 1-diphosphate + anthranilate</text>
            <dbReference type="Rhea" id="RHEA:11768"/>
            <dbReference type="ChEBI" id="CHEBI:16567"/>
            <dbReference type="ChEBI" id="CHEBI:18277"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="EC" id="2.4.2.18"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="3">Binds 2 magnesium ions per monomer.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 2/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the anthranilate phosphoribosyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.18" evidence="3"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96370.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0825"/>
    <dbReference type="EnsemblBacteria" id="AEF96370">
        <property type="protein sequence ID" value="AEF96370"/>
        <property type="gene ID" value="Metig_0825"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0825"/>
    <dbReference type="HOGENOM" id="CLU_034315_2_1_2"/>
    <dbReference type="OMA" id="GPMTNPA"/>
    <dbReference type="UniPathway" id="UPA00035">
        <property type="reaction ID" value="UER00041"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004048">
        <property type="term" value="F:anthranilate phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000162">
        <property type="term" value="P:tryptophan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1030.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00211">
        <property type="entry name" value="TrpD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005940">
        <property type="entry name" value="Anthranilate_Pribosyl_Tfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000312">
        <property type="entry name" value="Glycosyl_Trfase_fam3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017459">
        <property type="entry name" value="Glycosyl_Trfase_fam3_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036320">
        <property type="entry name" value="Glycosyl_Trfase_fam3_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035902">
        <property type="entry name" value="Nuc_phospho_transferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43285">
        <property type="entry name" value="PTHR43285"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02885">
        <property type="entry name" value="Glycos_trans_3N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00591">
        <property type="entry name" value="Glycos_transf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47648">
        <property type="entry name" value="SSF47648"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52418">
        <property type="entry name" value="SSF52418"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01245">
        <property type="entry name" value="trpD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="3" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="1 3 6" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="3" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3 6" id="KW-0808">Transferase</keyword>
    <keyword evidence="3" id="KW-0822">Tryptophan biosynthesis</keyword>
    <feature type="domain" description="Glycos_trans_3N" evidence="5">
        <location>
            <begin position="4"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="domain" description="Glycos_transf_3" evidence="4">
        <location>
            <begin position="74"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="region of interest" description="Phosphoribosylpyrophosphate binding" evidence="3">
        <location>
            <begin position="83"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="region of interest" description="Phosphoribosylpyrophosphate binding" evidence="3">
        <location>
            <begin position="90"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="region of interest" description="Phosphoribosylpyrophosphate binding" evidence="3">
        <location>
            <begin position="108"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="80"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="88"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="92"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="111"/>
        </location>
        <ligand>
            <name>anthranilate</name>
            <dbReference type="ChEBI" id="CHEBI:16567"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="120"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="166"/>
        </location>
        <ligand>
            <name>anthranilate</name>
            <dbReference type="ChEBI" id="CHEBI:16567"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="224"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00591"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEF96370.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="336" mass="36033" checksum="78721EA45496061B" modified="2011-07-27" version="1">MITDAIKKVVEFKDLNESEAIDVMNEIMSGKATPAQIASILTALRMKGETPVEIASFAKVMREFATKINPKVDKLVDTCGTGGDNLNTFNISTTVAFVVAGCGVAVAKHGNRSVSSKCGSADVLEALGVNLDLPPKKVEECIEKVGIGFLFAPLYHSAMKHALPVRKEIGIRTVFNVLGPLTNPANAEYQVVGVYDASLTEKIAEVLKLLGLKGAMVVHGSGMDEITTTGETKISELKDGEIKTYTITPERFGLKRADIDELKGGDAKENANILKRILEGEEGAKRDIVLLNAAATLYVCGEAKSLEEGIKMAEKSIDSGKAMEKLEKLVEFTNSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-06-02" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>F6BD64</accession>
    <name>F6BD64_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_0882</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96425.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799027.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96425">
        <property type="protein sequence ID" value="AEF96425"/>
        <property type="gene ID" value="Metig_0882"/>
    </dbReference>
    <dbReference type="GeneID" id="10643726"/>
    <dbReference type="KEGG" id="mig:Metig_0882"/>
    <dbReference type="HOGENOM" id="CLU_1590906_0_0_2"/>
    <dbReference type="OMA" id="FITARIN"/>
    <dbReference type="OrthoDB" id="80640at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF96425.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="164" mass="19533" checksum="CAE579DCD55851A6" modified="2011-07-27" version="1">MDVYEILFQKCLEYEVLLDDEKIPLWKLKKEDLDKVNFGLPWENLQDLAIYLYELKKEQQRSKELIKCDIAEILVGIAFLKPKKSGSLIADESLGINTCLNYLSELITARINCITRYYYLMKKPHNTDIFDEIILKFPQKKDIRAKNINDLREIVYKLRSYFEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDA7</accession>
    <name>F6BDA7_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">CDP-archaeol synthase</fullName>
            <ecNumber evidence="6">2.7.7.67</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">CDP-2,3-bis-(O-geranylgeranyl)-sn-glycerol synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">carS</name>
        <name evidence="7" type="ordered locus">Metig_0925</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes the formation of CDP-2,3-bis-(O-geranylgeranyl)-sn-glycerol (CDP-archaeol) from 2,3-bis-(O-geranylgeranyl)-sn-glycerol 1-phosphate (DGGGP) and CTP. This reaction is the third ether-bond-formation step in the biosynthesis of archaeal membrane lipids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>2,3-bis-O-(geranylgeranyl)-sn-glycerol 1-phosphate + CTP + H(+) = CDP-2,3-bis-O-(geranylgeranyl)-sn-glycerol + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:25690"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:58837"/>
            <dbReference type="ChEBI" id="CHEBI:58838"/>
            <dbReference type="EC" id="2.7.7.67"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="6">Membrane lipid metabolism; glycerophospholipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cell membrane</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the CDP-archaeol synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.67" evidence="6"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96468.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799070.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0925"/>
    <dbReference type="EnsemblBacteria" id="AEF96468">
        <property type="protein sequence ID" value="AEF96468"/>
        <property type="gene ID" value="Metig_0925"/>
    </dbReference>
    <dbReference type="GeneID" id="10643771"/>
    <dbReference type="KEGG" id="mig:Metig_0925"/>
    <dbReference type="HOGENOM" id="CLU_105710_0_0_2"/>
    <dbReference type="OMA" id="GKTWRGT"/>
    <dbReference type="OrthoDB" id="124973at2157"/>
    <dbReference type="UniPathway" id="UPA00940"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005887">
        <property type="term" value="C:integral component of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043338">
        <property type="term" value="F:CTP:2,3-di-O-geranylgeranyl-sn-glycero-1-phosphate cytidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046474">
        <property type="term" value="P:glycerophospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01117">
        <property type="entry name" value="CDP_archaeol_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032690">
        <property type="entry name" value="CarS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002726">
        <property type="entry name" value="CarS_archaea"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39650">
        <property type="entry name" value="PTHR39650"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01864">
        <property type="entry name" value="CarS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="6" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="54"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="93"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="150"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEF96468.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="179" mass="19636" checksum="4D10610893C65B5E" modified="2011-07-27" version="1">MDIIGLVFTSFWYILPAYIANATACIFGGGTPLDLGKNFIDGRRLVGDGVTFKGTFSGVLCGTIAAILQGVILNLKIVNVPMVFYSNIFEYTFLGFLLSSGALFGDMVGSFIKRRLGIKQGNPAPILDQLNFVFGAILFTYPFSPIPLNMVITICIITPVIHLTSNIIAYKLKIKKVWW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDD8</accession>
    <name>F6BDD8_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">50S ribosomal protein L37Ae</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">Ribosomal protein L43e</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2" type="primary">rpl37ae</name>
        <name evidence="3" type="ordered locus">Metig_0956</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Binds to the 23S rRNA.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="2">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eukaryotic ribosomal protein eL43 family. Putative zinc-binding subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96499.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799101.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0956"/>
    <dbReference type="EnsemblBacteria" id="AEF96499">
        <property type="protein sequence ID" value="AEF96499"/>
        <property type="gene ID" value="Metig_0956"/>
    </dbReference>
    <dbReference type="GeneID" id="10643802"/>
    <dbReference type="KEGG" id="mig:Metig_0956"/>
    <dbReference type="HOGENOM" id="CLU_141199_2_0_2"/>
    <dbReference type="OMA" id="ISTGIWQ"/>
    <dbReference type="OrthoDB" id="122290at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070180">
        <property type="term" value="F:large ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00327">
        <property type="entry name" value="Ribosomal_L37Ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002674">
        <property type="entry name" value="Ribosomal_L37ae"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011331">
        <property type="entry name" value="Ribosomal_L37ae/L37e"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011332">
        <property type="entry name" value="Ribosomal_zn-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01780">
        <property type="entry name" value="Ribosomal_L37ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57829">
        <property type="entry name" value="SSF57829"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00280">
        <property type="entry name" value="eL43_euk_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 3" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2" id="KW-0699">rRNA-binding</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="zinc finger region" description="C4-type" evidence="2">
        <location>
            <begin position="40"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="40"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="43"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="58"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="61"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00327"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF96499.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="95" mass="10558" checksum="1E17B1884A9B24F2" modified="2011-07-27" version="1">MAFSHTKKVGPAGRFGARYGRKIRVRVRDVEIKAKKKYKCPVCGFQKLRRVSTSIWMCGKCGAKIAGGAYTPETGAGKVVTKAIRRVIESKKREI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-06-02" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDF0</accession>
    <name>F6BDF0_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Class III signal peptide-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metig_0968</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96511.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96511">
        <property type="protein sequence ID" value="AEF96511"/>
        <property type="gene ID" value="Metig_0968"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0968"/>
    <dbReference type="HOGENOM" id="CLU_197251_1_0_2"/>
    <dbReference type="OMA" id="SIVGFYY"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007166">
        <property type="entry name" value="Class3_signal_pept_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026451">
        <property type="entry name" value="ClassIII_w_PIP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04021">
        <property type="entry name" value="Class_IIIsignal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR04205">
        <property type="entry name" value="classIII_w_PIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="37"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEF96511.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="75" mass="8009" checksum="7D57AD65A2B0159A" modified="2011-07-27" version="1">MKKIISNKGQISLEFALLFAVAVSVAAIAGFYYVKSTKSSSITSEKSSVKGSTTTSNKDMELVDSIEEVITNEGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDF2</accession>
    <name>F6BDF2_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0970</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96513.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799115.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0970"/>
    <dbReference type="EnsemblBacteria" id="AEF96513">
        <property type="protein sequence ID" value="AEF96513"/>
        <property type="gene ID" value="Metig_0970"/>
    </dbReference>
    <dbReference type="GeneID" id="10643816"/>
    <dbReference type="KEGG" id="mig:Metig_0970"/>
    <dbReference type="HOGENOM" id="CLU_125809_0_0_2"/>
    <dbReference type="OMA" id="NVLNMAK"/>
    <dbReference type="OrthoDB" id="114198at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="15"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96513.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="149" mass="16671" checksum="379A2BE2B1D6AAA1" modified="2011-07-27" version="1">MVIRLSLISRNKGQITLDAILAVMFLLLVSAFIFYNVSNTVSHLKDAEIVDRVYVIADVFENYALLSYSKNVNITTELKPIGLKNYTIYFGDKKIVVDTTKTIVFIPTSGGVRVEGDVEPSGQNLSNIINITYGNNEFYVLKNISIEIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDF9</accession>
    <name>F6BDF9_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Cysteine-rich small domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0977</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96520.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0977"/>
    <dbReference type="EnsemblBacteria" id="AEF96520">
        <property type="protein sequence ID" value="AEF96520"/>
        <property type="gene ID" value="Metig_0977"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0977"/>
    <dbReference type="HOGENOM" id="CLU_1998728_0_0_2"/>
    <dbReference type="OMA" id="LWCYCPF"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007212">
        <property type="entry name" value="Zf-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04071">
        <property type="entry name" value="zf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="zf-like" evidence="1">
        <location>
            <begin position="18"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96520.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="129" mass="15148" checksum="7A45172D2DC6AA2A" modified="2011-07-27" version="1">MVMIELAKKHLKKVLEVCGANRNCEYYPCHFDGQVCLWCYCPFYPCEDENFGEWIERKDGTKVWSCMNCHWIHKPEVACEVLKEILELTKDKSIDEAIGLLDDREVLLKIRNKVLEKFKKEKKDDTGGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDG5</accession>
    <name>F6BDG5_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_0984</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96526.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799128.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96526">
        <property type="protein sequence ID" value="AEF96526"/>
        <property type="gene ID" value="Metig_0984"/>
    </dbReference>
    <dbReference type="GeneID" id="10643831"/>
    <dbReference type="KEGG" id="mig:Metig_0984"/>
    <dbReference type="HOGENOM" id="CLU_085959_0_0_2"/>
    <dbReference type="OMA" id="YFIMGTH"/>
    <dbReference type="OrthoDB" id="49229at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF96526.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="256" mass="30402" checksum="5B2118C8FA5B5D4D" modified="2011-07-27" version="1">MKERLLVLAKAAPVQSAKYRYLVCVAGITESGEWRRIFPIPWEVFSGKSKNKFTKKQWIEYELVEDKSPDGRPESRKIKKQSITPLHTESYKSIKKLLEERLTDMETLQNKKRQECSLGVVKPIIKDMVWTDREVSSKDDVTQLTLDGTEFIRLEPLDKKFQYVFKCSPTCPTEHKIMCEDWEIEELYRKLKKKYDENKACNLVRDKFLNKIYYEGHTYFIVGTHNRWNTWLIVSVLYPKKEDLKILSIKSLDEFM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDI6</accession>
    <name>F6BDI6_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">4Fe-4S ferredoxin iron-sulfur binding domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1005</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96547.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799149.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1005"/>
    <dbReference type="EnsemblBacteria" id="AEF96547">
        <property type="protein sequence ID" value="AEF96547"/>
        <property type="gene ID" value="Metig_1005"/>
    </dbReference>
    <dbReference type="GeneID" id="10643859"/>
    <dbReference type="KEGG" id="mig:Metig_1005"/>
    <dbReference type="HOGENOM" id="CLU_1521877_0_0_2"/>
    <dbReference type="OMA" id="NCKLCIQ"/>
    <dbReference type="OrthoDB" id="105930at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00037">
        <property type="entry name" value="Fer4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13237">
        <property type="entry name" value="Fer4_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12800">
        <property type="entry name" value="Fer4_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="43"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="75"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="96"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="124"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="153"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="187"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96547.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="210" mass="23990" checksum="C42B48FFBE5DD24C" modified="2011-07-27" version="1">MKRDQNEVDEFYKFLKTKSILKLIIKNLFGKKTEFLDTNKRTEKPHLDNCIGCGLCIDVCPTNAIEIFKFREIICSCCGVCVEVCPNNAIEKDRFTINAEKCTKCGYCVLFCSIPYIKNEIPKPKTPVITSECNKCMLCIKKCENKAIEFKDNKIIIDEEKCKNCLKCVEFCPMKAILSPEESINSCIVKVDINSCIFCKECVEVCPLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDJ8</accession>
    <name>F6BDJ8_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Nascent polypeptide-associated complex protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">nac</name>
        <name evidence="7" type="ordered locus">Metig_1017</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Contacts the emerging nascent chain on the ribosome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer. Interacts with the ribosome. Binds ribosomal RNA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the NAC-alpha family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96559.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799161.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1017"/>
    <dbReference type="EnsemblBacteria" id="AEF96559">
        <property type="protein sequence ID" value="AEF96559"/>
        <property type="gene ID" value="Metig_1017"/>
    </dbReference>
    <dbReference type="GeneID" id="10643871"/>
    <dbReference type="KEGG" id="mig:Metig_1017"/>
    <dbReference type="HOGENOM" id="CLU_146475_1_0_2"/>
    <dbReference type="OMA" id="PRKMKQM"/>
    <dbReference type="OrthoDB" id="105165at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.70.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00814">
        <property type="entry name" value="NAC_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044034">
        <property type="entry name" value="NAC-like_UBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038187">
        <property type="entry name" value="NAC_A/B_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005231">
        <property type="entry name" value="NAC_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002715">
        <property type="entry name" value="Nas_poly-pep-assoc_cplx_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19026">
        <property type="entry name" value="HYPK_UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01849">
        <property type="entry name" value="NAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01407">
        <property type="entry name" value="NAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="SSF46934"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00264">
        <property type="entry name" value="TIGR00264"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51151">
        <property type="entry name" value="NAC_AB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="1 4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="NAC-A/B" evidence="6">
        <location>
            <begin position="9"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="70"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEF96559.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="125" mass="14473" checksum="2643A75C663E3E3A" modified="2011-07-27" version="1">MFPGGRLNPRMLKQMQKMMKDFGMDSEDLNVVRVIFEFEDEEWVFEKPKVQVVDIFGVKTYSITGKPKKIKKEEKEIKDEDVKVEITEEDVELVANQCNVSKEEARKALEECNGDIAEAILKLSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDQ8</accession>
    <name>F6BDQ8_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DNA primase large subunit PriL</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">priL</name>
        <name evidence="8" type="ordered locus">Metig_1080</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Regulatory subunit of DNA primase, an RNA polymerase that catalyzes the synthesis of short RNA molecules used as primers for DNA polymerase during DNA replication. Stabilizes and modulates the activity of the small subunit, increasing the rate of DNA synthesis, and conferring RNA synthesis capability. The DNA polymerase activity may enable DNA primase to also catalyze primer extension after primer synthesis. May also play a role in DNA repair.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="7">Binds 1 [4Fe-4S] cluster.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Heterodimer of a small subunit (PriS) and a large subunit (PriL).</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the eukaryotic-type primase large subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96619.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799220.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1080"/>
    <dbReference type="EnsemblBacteria" id="AEF96619">
        <property type="protein sequence ID" value="AEF96619"/>
        <property type="gene ID" value="Metig_1080"/>
    </dbReference>
    <dbReference type="GeneID" id="10643934"/>
    <dbReference type="KEGG" id="mig:Metig_1080"/>
    <dbReference type="HOGENOM" id="CLU_759942_0_0_2"/>
    <dbReference type="OMA" id="HYARRSF"/>
    <dbReference type="OrthoDB" id="29893at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990077">
        <property type="term" value="C:primosome complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003896">
        <property type="term" value="F:DNA primase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00701">
        <property type="entry name" value="DNA_primase_lrg_arc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007238">
        <property type="entry name" value="DNA_primase_lsu_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023642">
        <property type="entry name" value="DNA_primase_lsu_PriL"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04104">
        <property type="entry name" value="DNA_primase_lrg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 7" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="3 7" id="KW-0235">DNA replication</keyword>
    <keyword evidence="5 7" id="KW-0408">Iron</keyword>
    <keyword evidence="6 7" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 7" id="KW-0639">Primosome</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="7">
        <location>
            <position position="195"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="295"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="313"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="319"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEF96619.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="349" mass="41514" checksum="37A875B85B4A8146" modified="2011-07-27" version="1">MFWEHLDKFKDVDFVEAVKENRRGIEYLLKYIFEDNDTYHKFFENHKEGDALIKYLTLILIAYTRFHNAYIKKFSEKYWKDILNNLPNEINVWEYIKIASLSKNNDLHLERLDLKNGKVNIYEIKKIYAKEFIRVKLKELIDKIKKVKLPEDETIKNILNDIDNYLKDKVKFEGIEGIKALDYKGEIPLDWHPPCIRGILNDILSGGSPSHYARRSFVVYWFCAKFNPNLRPLNDKGELVNISALDIASEEEIEKFIDDLINMLFKNVEDFDEKKTRYYISHNIGYKVADHITHCEYCKNWKDDGGKGLSYYCNPDDICKKRGIIHPLDYLCYNINMHLKLKSKDKKQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDR0</accession>
    <name>F6BDR0_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Geranylgeranylglyceryl phosphate synthase</fullName>
            <shortName evidence="9">GGGP synthase</shortName>
            <shortName evidence="9">GGGPS</shortName>
            <ecNumber evidence="9">2.5.1.41</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">(S)-3-O-geranylgeranylglyceryl phosphate synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Phosphoglycerol geranylgeranyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Metig_1082</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Prenyltransferase that catalyzes the transfer of the geranylgeranyl moiety of geranylgeranyl diphosphate (GGPP) to the C3 hydroxyl of sn-glycerol-1-phosphate (G1P). This reaction is the first ether-bond-formation step in the biosynthesis of archaeal membrane lipids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>(2E,6E,10E)-geranylgeranyl diphosphate + sn-glycerol 1-phosphate = diphosphate + sn-3-O-(geranylgeranyl)glycerol 1-phosphate</text>
            <dbReference type="Rhea" id="RHEA:23404"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57677"/>
            <dbReference type="ChEBI" id="CHEBI:57685"/>
            <dbReference type="ChEBI" id="CHEBI:58756"/>
            <dbReference type="EC" id="2.5.1.41"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="9">Membrane lipid metabolism; glycerophospholipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the GGGP/HepGP synthase family. Group II subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.41" evidence="9"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96621.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799222.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1082"/>
    <dbReference type="EnsemblBacteria" id="AEF96621">
        <property type="protein sequence ID" value="AEF96621"/>
        <property type="gene ID" value="Metig_1082"/>
    </dbReference>
    <dbReference type="GeneID" id="10643936"/>
    <dbReference type="KEGG" id="mig:Metig_1082"/>
    <dbReference type="HOGENOM" id="CLU_068610_0_0_2"/>
    <dbReference type="OMA" id="ADAIFFM"/>
    <dbReference type="OrthoDB" id="94629at2157"/>
    <dbReference type="UniPathway" id="UPA00940"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047294">
        <property type="term" value="F:phosphoglycerol geranylgeranyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046474">
        <property type="term" value="P:glycerophospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02812">
        <property type="entry name" value="PcrB_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.390">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00112">
        <property type="entry name" value="GGGP_HepGP_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038597">
        <property type="entry name" value="GGGP/HepGP_synthase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008205">
        <property type="entry name" value="GGGP_HepGP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010946">
        <property type="entry name" value="GGGP_synth"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01884">
        <property type="entry name" value="PcrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01769">
        <property type="entry name" value="GGGP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01768">
        <property type="entry name" value="GGGP-family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 9" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="6 9" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="5 9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 9" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="8 9" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Glycerol-1-phosphate binding" evidence="9">
        <location>
            <begin position="172"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="region of interest" description="Glycerol-1-phosphate binding" evidence="9">
        <location>
            <begin position="203"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="region of interest" description="Glycerol-1-phosphate binding" evidence="9">
        <location>
            <begin position="225"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="28"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="53"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AEF96621.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="255" mass="28065" checksum="E87224CA4731EAE2" modified="2011-07-27" version="1">MEIKIGKVEKKLNKIIEEEGAIYLVLIDPDEKNIEEIAEKVKDYADAIMIGGSIGVINLDEVTKKIKEITKLPTILFPGNVDGLTQYADAVFFMSLMNSTNPYWIVNAPTLGSLTIKKFGLEPIPMAYLCVEPAKRTAVGFVGEINEIPQRKPEIAAMYCLSAKYFGMRWAYLEAGSGAEYPVSNEMIAISKKLSGINIIVGGGIRKPEIAYEKVLSGADAVVTGNLLEKDPNAVEEMYDAIKKAGREKLKNQKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDT2</accession>
    <name>F6BDT2_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1104</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96643.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1104"/>
    <dbReference type="EnsemblBacteria" id="AEF96643">
        <property type="protein sequence ID" value="AEF96643"/>
        <property type="gene ID" value="Metig_1104"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_1104"/>
    <dbReference type="HOGENOM" id="CLU_183480_0_0_2"/>
    <dbReference type="OMA" id="LTNYVYT"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043941">
        <property type="entry name" value="EMC6-arch"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19094">
        <property type="entry name" value="EMC6_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="9"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="34"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="69"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96643.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="93" mass="10149" checksum="1633DAB674011C52" modified="2011-07-27" version="1">MMIDLEGKVGIIHAIGGAIAGYLTNYVYTVGLGSLSGIAAFVFMIVALLITGHITAMIFGRESMNQKQWFGSGVVPFFFVWVVFWVLKFNGVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDT5</accession>
    <name>F6BDT5_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Transposase, IS605 OrfB family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Metig_1107</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">In the C-terminal section; belongs to the transposase 35 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96646.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799247.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1107"/>
    <dbReference type="EnsemblBacteria" id="AEF96646">
        <property type="protein sequence ID" value="AEF96646"/>
        <property type="gene ID" value="Metig_1107"/>
    </dbReference>
    <dbReference type="GeneID" id="10643965"/>
    <dbReference type="KEGG" id="mig:Metig_1107"/>
    <dbReference type="HOGENOM" id="CLU_032903_3_4_2"/>
    <dbReference type="OMA" id="ELYHIHR"/>
    <dbReference type="OrthoDB" id="75656at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032196">
        <property type="term" value="P:transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001959">
        <property type="entry name" value="Transposase_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010095">
        <property type="entry name" value="Transposase_IS605_OrfB_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01385">
        <property type="entry name" value="OrfB_IS605"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07282">
        <property type="entry name" value="OrfB_Zn_ribbon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01766">
        <property type="entry name" value="tspaseT_teng_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0815">Transposition</keyword>
    <feature type="domain" description="OrfB_IS605" evidence="5">
        <location>
            <begin position="193"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="domain" description="OrfB_Zn_ribbon" evidence="6">
        <location>
            <begin position="304"/>
            <end position="365"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF07282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEF96646.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="412" mass="48752" checksum="1F6840B6A28F9F88" modified="2011-07-27" version="1">MPNKTKPKETQELSYQVVLSYKVSHNYPLKTFLIECKDKLNECIDMIWNNIEYTKKDKPKLPKSNEFKRNLRNKLLENWSYASHYIDGIIKTSYSILQSWASNYKRGYRTKTKPVVERLFVRVKTTLIKYDKERAEIRITIKPRKEYLTLNIKNEWFFDKVKNLTIGEIILKENEAFLTFKDNLNYSDKDIIVGVDSNLKSLDLFHPIEGWIRIDLTELHRLKEVYDRKIDFLKKLLKKCPLRVLRKINRLFERRRNRVKDFLHKLTTQLSRLFPDAIFVFEDLNKRKMYKNKHFNRRIDRANWNGIIEKISYKAIIILVNPAYTSTTCPICGSRMESQEGQVVCYNCSNSFNRQVVGCFNIFKRGLNVIKRFMGGSGVTTTGAEVSAKRLMTPNPNVVYYVDLNGKYLKCI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDU5</accession>
    <name>F6BDU5_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_1117</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96656.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799257.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1117"/>
    <dbReference type="EnsemblBacteria" id="AEF96656">
        <property type="protein sequence ID" value="AEF96656"/>
        <property type="gene ID" value="Metig_1117"/>
    </dbReference>
    <dbReference type="GeneID" id="10643975"/>
    <dbReference type="KEGG" id="mig:Metig_1117"/>
    <dbReference type="HOGENOM" id="CLU_114385_0_0_2"/>
    <dbReference type="OMA" id="AVFNKNR"/>
    <dbReference type="OrthoDB" id="127282at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.130.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036974">
        <property type="entry name" value="PUA_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040598">
        <property type="entry name" value="UPF0113_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005155">
        <property type="entry name" value="UPF0113_PUA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03657">
        <property type="entry name" value="UPF0113"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17833">
        <property type="entry name" value="UPF0113_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UPF0113_N" evidence="2">
        <location>
            <begin position="4"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="domain" description="UPF0113" evidence="1">
        <location>
            <begin position="98"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF17833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF96656.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="163" mass="18976" checksum="BD49B430C1AE2C31" modified="2011-07-27" version="1">MKFRALTKKEIGIIKHELKRFVDEEYLKTFPFENLYALVGNWVNVVYATKEVIKNLKNFEKVYSFGIIFGEFSRKNKNRFLLSLEGMSLIADGICKNYAIVNEKGETMFLYGRDIFKSSILEIHGKGKVAVFNKYRDFLGIGKYDGKMIKNIKDKGWYLREGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDX3</accession>
    <name>F6BDX3_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1145</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96684.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799285.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1145"/>
    <dbReference type="EnsemblBacteria" id="AEF96684">
        <property type="protein sequence ID" value="AEF96684"/>
        <property type="gene ID" value="Metig_1145"/>
    </dbReference>
    <dbReference type="GeneID" id="10644003"/>
    <dbReference type="KEGG" id="mig:Metig_1145"/>
    <dbReference type="HOGENOM" id="CLU_079270_4_0_2"/>
    <dbReference type="OMA" id="GYMLEVM"/>
    <dbReference type="OrthoDB" id="96137at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025098">
        <property type="entry name" value="DUF4013"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13197">
        <property type="entry name" value="DUF4013"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="62"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="101"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="148"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="173"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96684.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="203" mass="22508" checksum="84538CF749E96668" modified="2011-07-27" version="1">MDIIEGLKFPMDDEEWVKKVIIGGIIGIIPIVNLIVGGYFVETMKYVITGKKVLPEWENWGGKFITGICVFIIGLIYFAIPLVVMILLGGLGALVGKNSSIVGFVLGFVLFIVFGFAMPMAIANYAAKEKLSAAFEFEEILGRIKSVIGDYLLAYVVLFILVFVLDLVSMIPIIGWILSIFAMFYLSLVAVYYFGTLYRESSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>F6BE05</accession>
    <name>F6BE05_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">TfuA-like core domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1178</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96716.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799315.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1178"/>
    <dbReference type="EnsemblBacteria" id="AEF96716">
        <property type="protein sequence ID" value="AEF96716"/>
        <property type="gene ID" value="Metig_1178"/>
    </dbReference>
    <dbReference type="GeneID" id="10644038"/>
    <dbReference type="KEGG" id="mig:Metig_1178"/>
    <dbReference type="HOGENOM" id="CLU_090311_0_0_2"/>
    <dbReference type="OMA" id="MISFREM"/>
    <dbReference type="OrthoDB" id="72543at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012924">
        <property type="entry name" value="TfuA_core"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07812">
        <property type="entry name" value="TfuA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TfuA" evidence="1">
        <location>
            <begin position="48"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96716.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="211" mass="24129" checksum="F0F130F9C1988089" modified="2011-07-27" version="1">MKIAIFSKLTLKEDEIKAVIDADVFPPIKRGDLLNPIIDKYEVIGIIDGVFLQNTAVGHREILHRLKEGKVIFGAGSMGALRASELDVHGMIGVGKVYKLYKEGVIVDDDEVAVTFDDNFNQISFSLVSFRFMVKNAVEKGLISKEEGDLLIKIAKSLYYPLRTFKNVLDRTDFSGEKKEKLLEFFKNEEDIKRKDAFEMLYRIRDFIEKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>F6BE25</accession>
    <name>F6BE25_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Hydrogen dehydrogenase</fullName>
            <ecNumber evidence="3">1.12.1.2</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_1198</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.12.1.2" evidence="3"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96736.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799335.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1198"/>
    <dbReference type="EnsemblBacteria" id="AEF96736">
        <property type="protein sequence ID" value="AEF96736"/>
        <property type="gene ID" value="Metig_1198"/>
    </dbReference>
    <dbReference type="GeneID" id="10644058"/>
    <dbReference type="KEGG" id="mig:Metig_1198"/>
    <dbReference type="HOGENOM" id="CLU_053270_0_0_2"/>
    <dbReference type="OMA" id="VKIATTW"/>
    <dbReference type="OrthoDB" id="44934at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047985">
        <property type="term" value="F:hydrogen dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.700">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006137">
        <property type="entry name" value="NADH_UbQ_OxRdtase-like_20kDa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037024">
        <property type="entry name" value="NiFe_Hase_small_N_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01058">
        <property type="entry name" value="Oxidored_q6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Oxidored_q6" evidence="2">
        <location>
            <begin position="12"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF96736.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="303" mass="33060" checksum="22A4ACBAD8E33265" modified="2011-07-27" version="1">MVKIATTWLCCCSGCHISLLDLHEELLNLLENVELVHCPVLMDVKEIPDEIDVALIEGGIRNEENLEIAKEMRKKAKIVIAFGTCATFGGVPGLGNLYSNDEILEKVYKTTLTTKNDNGIIPNEEVPPLTSRVKPLSEVIDVDYFIPGCPPNPEIIANIIKALLEGKEPELPKKNMCDECPRKKSDDGVAIETIKRKYEGNPDPEKCLLEQGYLCLGPATREGCGAACPSVGVPCSGCNGPTDAIVDQGAKMISALCSDFGIDKDREVDPTILPKSIKDKIGCFYKFTLPSALIPIRIKHSHD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2020-06-17" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>F6BE27</accession>
    <name>F6BE27_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0001</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95563.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF95563">
        <property type="protein sequence ID" value="AEF95563"/>
        <property type="gene ID" value="Metig_0001"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0001"/>
    <dbReference type="HOGENOM" id="CLU_3282848_0_0_2"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF95563.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="40" mass="4544" checksum="9406D8D0F93A48FA" modified="2011-07-27" version="1">MGVNLAIIIVVTMVIYNFGFMEHLNILINNILTLNKNNKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F6BE41</accession>
    <name>F6BE41_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Methyltransf_30 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0015</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95577.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798187.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0015"/>
    <dbReference type="EnsemblBacteria" id="AEF95577">
        <property type="protein sequence ID" value="AEF95577"/>
        <property type="gene ID" value="Metig_0015"/>
    </dbReference>
    <dbReference type="GeneID" id="10642850"/>
    <dbReference type="KEGG" id="mig:Metig_0015"/>
    <dbReference type="HOGENOM" id="CLU_052777_0_0_2"/>
    <dbReference type="OMA" id="ICYGLGY"/>
    <dbReference type="OrthoDB" id="42522at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016645">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-NH group of donors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008471">
        <property type="entry name" value="MnmC-like_methylTransf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05430">
        <property type="entry name" value="Methyltransf_30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Methyltransf_30" evidence="1">
        <location>
            <begin position="154"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF95577.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="352" mass="40609" checksum="E9BC004E3F004204" modified="2011-07-27" version="1">MLPNKKAIEIIRKYMGLAIDNNVFERDDFDEFGRKLVKELIDKNLLVKTEDGTYTLKAEDENELMHSKIGALKEAVEKFAIPSDIKGRRNPKILDLCSGFGYNAVAALHFNKDAEIDMVELCREVLFLSLCLNIPFKEHEIIKEAIKNFFKGKDAENSKIRIFNEDARKAIKRLDKKYDIVFHDAFSPQRDPILYTVDFLKEIYKKMNDNGVLISYSSSIPFRSGLVEAGFVISEGPSVGRKRGATIAYKNPSFDVSRIPEVDERVIALSTVGVPYRDEHLNATKEEIIKNREIEREKFKRKLMDLGKYYSTKKIKKGNIDEEFLKIQKMDLNSSEIIKRMRTLLLNPKNKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>F6BE65</accession>
    <name>F6BE65_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Pre-mRNA processing ribonucleoprotein, binding domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Metig_1204</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NOP5/NOP56 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96742.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799341.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1204"/>
    <dbReference type="EnsemblBacteria" id="AEF96742">
        <property type="protein sequence ID" value="AEF96742"/>
        <property type="gene ID" value="Metig_1204"/>
    </dbReference>
    <dbReference type="GeneID" id="10644064"/>
    <dbReference type="KEGG" id="mig:Metig_1204"/>
    <dbReference type="HOGENOM" id="CLU_015495_1_0_2"/>
    <dbReference type="OMA" id="REWYSLH"/>
    <dbReference type="OrthoDB" id="25339at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031428">
        <property type="term" value="C:box C/D RNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030515">
        <property type="term" value="F:snoRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.246.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.660">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029012">
        <property type="entry name" value="Helix_hairpin_bin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045056">
        <property type="entry name" value="Nop56/Nop58"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042239">
        <property type="entry name" value="Nop_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002687">
        <property type="entry name" value="Nop_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036070">
        <property type="entry name" value="Nop_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012976">
        <property type="entry name" value="NOSIC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10894">
        <property type="entry name" value="PTHR10894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01798">
        <property type="entry name" value="Nop"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00931">
        <property type="entry name" value="NOSIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89124">
        <property type="entry name" value="SSF89124"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51358">
        <property type="entry name" value="NOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5" id="KW-0543">Viral nucleoprotein</keyword>
    <keyword evidence="5" id="KW-0946">Virion</keyword>
    <feature type="domain" description="Nop" evidence="4">
        <location>
            <begin position="249"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="361"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="221"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="366"/>
            <end position="410"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEF96742.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="418" mass="48332" checksum="897609343F199486" modified="2011-07-27" version="1">MLFIAFSPFGAFALKEKESYTYTIDDIEYYKIFNEEEIPKILYDLKRGNVQGIIEDLKKEWNVDDEVDVEVFGKPNVAGEIVRANLYELGRKFGVFEDYNDFINKMNFWALELTKLSMKSFSQQKDKLIIQTVEALDDLDKTLNLLSERIREWYSLYFPEMDKIIKKHELYVNLITQFGEREKFTKSQLKKILPSNIAKELAESAKSSMGGDISEEDLRILRDMAEEIKRLYERRKELQSYLEDLMEEFAPNLTKVAGASLGARLISLAGGLEKLAKFPASTVQVLGAEKALFAHLREGAEPPKHGVIYQHPFIQSSPKYLRGKIARALACKISIAARADMFGNYIGDMLLEQLEKKVEEIRKRYPKPPKKKKKEKAKKGKKKEKKPKKEKPKKKKEKKKGKKGKIERKVIGKTKSRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>F6BED7</accession>
    <name>F6BED7_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 6">NH(3)-dependent NAD(+) synthetase</fullName>
            <ecNumber evidence="4 6">6.3.1.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">nadE</name>
        <name evidence="9" type="ordered locus">Metig_0037</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Catalyzes the ATP-dependent amidation of deamido-NAD to form NAD. Uses ammonia as a nitrogen source.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4 6">
            <text>ATP + deamido-NAD(+) + NH4(+) = AMP + diphosphate + H(+) + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:21188"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:58437"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.1.5"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4 7">Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from deamido-NAD(+) (ammonia route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4 5">Belongs to the NAD synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.1.5" evidence="4 6"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95598.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798207.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0037"/>
    <dbReference type="EnsemblBacteria" id="AEF95598">
        <property type="protein sequence ID" value="AEF95598"/>
        <property type="gene ID" value="Metig_0037"/>
    </dbReference>
    <dbReference type="GeneID" id="10642872"/>
    <dbReference type="KEGG" id="mig:Metig_0037"/>
    <dbReference type="HOGENOM" id="CLU_059327_1_1_2"/>
    <dbReference type="OMA" id="MAFLYDY"/>
    <dbReference type="OrthoDB" id="89944at2157"/>
    <dbReference type="UniPathway" id="UPA00253">
        <property type="reaction ID" value="UER00333"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004359">
        <property type="term" value="F:glutaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003952">
        <property type="term" value="F:NAD+ synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008795">
        <property type="term" value="F:NAD+ synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009435">
        <property type="term" value="P:NAD biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00553">
        <property type="entry name" value="NAD_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00193">
        <property type="entry name" value="NadE_ammonia_dep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022310">
        <property type="entry name" value="NAD/GMP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003694">
        <property type="entry name" value="NAD_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022926">
        <property type="entry name" value="NH(3)-dep_NAD(+)_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23090">
        <property type="entry name" value="PTHR23090"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02540">
        <property type="entry name" value="NAD_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00552">
        <property type="entry name" value="nadE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 5 9" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 5" id="KW-0520">NAD</keyword>
    <keyword evidence="2 4 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD_synthase" evidence="8">
        <location>
            <begin position="6"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="29"/>
            <end position="36"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="35"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="115"/>
        </location>
        <ligand>
            <name>deamido-NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58437"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="135"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="140"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="148"/>
        </location>
        <ligand>
            <name>deamido-NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58437"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="155"/>
        </location>
        <ligand>
            <name>deamido-NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58437"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="164"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="186"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="245"/>
            <end position="246"/>
        </location>
        <ligand>
            <name>deamido-NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58437"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004252"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AEF95598.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="255" mass="28504" checksum="44687A7DED8DC4DB" modified="2011-07-27" version="1">MNEEEIINKICTFIKEKVEEAKVNGVVLGLSGGIDSSLTAYLCVRALGKDKVLGLIMPEKNSNPKDREHAELVAKKLGIKYIISDITDILKAFGAGGYVPTREFDKKADANLKARIRMCILYYFANKYNLLVVGTGNKSEAYMGYATKYGDLGCDILPIGNLFKTDVRRLARYLGIPKEIIEKPPSAGLWEGQTDEEELGITYELLDKILMALEEGKDKEEISKELNVSISKIEEIMHRIEANKHKLLPPPKPEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEE9</accession>
    <name>F6BEE9_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Prephenate dehydratase</fullName>
            <ecNumber evidence="8">4.2.1.51</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Metig_0049</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="5">Amino-acid biosynthesis.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.51" evidence="8"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95610.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798219.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0049"/>
    <dbReference type="EnsemblBacteria" id="AEF95610">
        <property type="protein sequence ID" value="AEF95610"/>
        <property type="gene ID" value="Metig_0049"/>
    </dbReference>
    <dbReference type="GeneID" id="10642884"/>
    <dbReference type="KEGG" id="mig:Metig_0049"/>
    <dbReference type="HOGENOM" id="CLU_035008_0_2_2"/>
    <dbReference type="OMA" id="PLMIYRE"/>
    <dbReference type="OrthoDB" id="47176at2157"/>
    <dbReference type="UniPathway" id="UPA00275"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004664">
        <property type="term" value="F:prephenate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009094">
        <property type="term" value="P:L-phenylalanine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002912">
        <property type="entry name" value="ACT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001086">
        <property type="entry name" value="Preph_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018528">
        <property type="entry name" value="Preph_deHydtase_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01842">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00800">
        <property type="entry name" value="PDT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="SSF55021"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51671">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00857">
        <property type="entry name" value="PREPHENATE_DEHYDR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00858">
        <property type="entry name" value="PREPHENATE_DEHYDR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51171">
        <property type="entry name" value="PREPHENATE_DEHYDR_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="2" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="4 8" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-0584">Phenylalanine biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Prephenate dehydratase" evidence="6">
        <location>
            <begin position="1"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="domain" description="ACT" evidence="7">
        <location>
            <begin position="190"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00029440"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEF95610.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="270" mass="30764" checksum="896D046A84D887F5" modified="2011-07-27" version="1">MLYYLGPRGSFTEKAGKIFSKLISLPLQPCSTIYEIFENVDKNNAYGVVPSENSIEGSVTLTQDLLLEYDVKIFGEIDIDISHNLVGYDKDKIEIILSHPQALAQCRKYIKEHGWKTKAVSSTAKAAEIVAKEKDERLGAIASMEAAKLYGLKILDEDIQDYKNNKTRFILIGKETPNFNAEPIAYKTTIIIELKEDKPGALYHILKEFAERDINLTRIESRPSKKRLGTYVFYIDFESYEDEEGLFKSLNKNVAYMKYLGTYPVFGIEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEG1</accession>
    <name>F6BEG1_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Tungstate ABC transporter binding protein WtpA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0061</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial solute-binding protein 1 family. WtpA subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95622.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798231.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0061"/>
    <dbReference type="EnsemblBacteria" id="AEF95622">
        <property type="protein sequence ID" value="AEF95622"/>
        <property type="gene ID" value="Metig_0061"/>
    </dbReference>
    <dbReference type="GeneID" id="10642896"/>
    <dbReference type="KEGG" id="mig:Metig_0061"/>
    <dbReference type="HOGENOM" id="CLU_055936_0_0_2"/>
    <dbReference type="OMA" id="GYRTVMM"/>
    <dbReference type="OrthoDB" id="43889at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901359">
        <property type="term" value="F:tungstate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022498">
        <property type="entry name" value="ABC_trnspt_W-bd_WtpA"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03730">
        <property type="entry name" value="tungstate_WtpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF95622.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="357" mass="40614" checksum="5906DD75A5E2AA79" modified="2011-07-27" version="1">MIKSKWLLVLLIGAISVALCGCVNTSKEQPTQSTTPQTSQASNAKVEEKVLKIFHAGSLSVPFSEYEKMFEKEHPNIDVQREPAGSVKCVRKITDLGKKADILASADYSLIPQMMMPKYADWYVMFARNEIVLAYTDKSKYKDEINSTNWYEILNKPDVKFGFSNPNDDPCGYRSQMVMQLAEIYYKNPKIYDDLVLKNSNIKVEEENGTYTILVPQNVEVNTNKIVLRSKETDLLAPLEAGAYDYLFIYKSVAEQHHLKYIELPKEINLGYYEYEDAYKKVKLKLLGKNKTLVAKPIVYGLTIPKNAPHKKEAIEFVKMVLEHPEVFEKNGQPAINPAVGFGNIPEEIKPLVKVEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEH3</accession>
    <name>F6BEH3_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Probable ribosomal RNA small subunit methyltransferase A</fullName>
            <ecNumber evidence="6">2.1.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">16S rRNA dimethyladenosine transferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">16S rRNA dimethylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">S-adenosylmethionine-6-N',N'-adenosyl(rRNA) dimethyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">rsmA</name>
        <name evidence="6 9" type="synonym">ksgA</name>
        <name evidence="9" type="ordered locus">Metig_0073</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Specifically dimethylates two adjacent adenosines in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the class I-like SAM-binding methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="6 7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="6"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95634.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0073"/>
    <dbReference type="EnsemblBacteria" id="AEF95634">
        <property type="protein sequence ID" value="AEF95634"/>
        <property type="gene ID" value="Metig_0073"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0073"/>
    <dbReference type="HOGENOM" id="CLU_041220_0_2_2"/>
    <dbReference type="OMA" id="KEEEPYF"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000179">
        <property type="term" value="F:rRNA (adenine-N6,N6-)-dimethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00607">
        <property type="entry name" value="16SrRNA_methyltr_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001737">
        <property type="entry name" value="KsgA/Erm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023165">
        <property type="entry name" value="rRNA_Ade_diMease-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020596">
        <property type="entry name" value="rRNA_Ade_Mease_Trfase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020598">
        <property type="entry name" value="rRNA_Ade_methylase_Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011530">
        <property type="entry name" value="rRNA_adenine_dimethylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11727">
        <property type="entry name" value="PTHR11727"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00398">
        <property type="entry name" value="RrnaAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00650">
        <property type="entry name" value="rADc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00755">
        <property type="entry name" value="ksgA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01131">
        <property type="entry name" value="RRNA_A_DIMETH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51689">
        <property type="entry name" value="SAM_RNA_A_N6_MT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 6 7" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6 7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="1 6" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="4 6 7" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3 6 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="rADc" evidence="8">
        <location>
            <begin position="25"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6 7">
        <location>
            <position position="20"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6 7">
        <location>
            <position position="45"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6 7">
        <location>
            <position position="66"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6 7">
        <location>
            <position position="91"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6 7">
        <location>
            <position position="108"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00650"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AEF95634.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="272" mass="31394" checksum="5FA5E2DC3688C652" modified="2011-07-27" version="1">MNKKWGITIKPSKKLGQCFLIDKNFVKKAVDAANLTKDDVVLEIGLGRGILTEELAKNAKKVYVIEIDEKLRPYAEKITREYDNVDVIWGDALKVNFDELGFNKVVANLPYQISSPITFKLLKTDFDVAVLMYQYEFAKRMVAKEGTKDYGRLSVAVQYKADIEFICKVPPSAFSPRPKVNSAIVKIVKREPLFKVEDEEFFENVLRALFQHKNKTVRRALINSCHELGVEREKIKEILENIDFNVDERVFKLSPKEIGNLSNLLYNHLRYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEI4</accession>
    <name>F6BEI4_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 8">Diaminopimelate epimerase</fullName>
            <shortName evidence="8">DAP epimerase</shortName>
            <ecNumber evidence="4 8">5.1.1.7</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">PLP-independent amino acid racemase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">dapF</name>
        <name evidence="10" type="ordered locus">Metig_0084</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the stereoinversion of LL-2,6-diaminoheptanedioate (L,L-DAP) to meso-diaminoheptanedioate (meso-DAP), a precursor of L-lysine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>(2S,6S)-2,6-diaminoheptanedioate = meso-2,6-diaminoheptanedioate</text>
            <dbReference type="Rhea" id="RHEA:15393"/>
            <dbReference type="ChEBI" id="CHEBI:57609"/>
            <dbReference type="ChEBI" id="CHEBI:57791"/>
            <dbReference type="EC" id="5.1.1.7"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 8">Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; DL-2,6-diaminopimelate from LL-2,6-diaminopimelate: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the diaminopimelate epimerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="5.1.1.7" evidence="4 8"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95645.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798254.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0084"/>
    <dbReference type="EnsemblBacteria" id="AEF95645">
        <property type="protein sequence ID" value="AEF95645"/>
        <property type="gene ID" value="Metig_0084"/>
    </dbReference>
    <dbReference type="GeneID" id="10642919"/>
    <dbReference type="KEGG" id="mig:Metig_0084"/>
    <dbReference type="HOGENOM" id="CLU_053306_3_0_2"/>
    <dbReference type="OMA" id="HVAMRVH"/>
    <dbReference type="OrthoDB" id="61864at2157"/>
    <dbReference type="UniPathway" id="UPA00034">
        <property type="reaction ID" value="UER00025"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008837">
        <property type="term" value="F:diaminopimelate epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009089">
        <property type="term" value="P:lysine biosynthetic process via diaminopimelate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00197">
        <property type="entry name" value="DAP_epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018510">
        <property type="entry name" value="DAP_epimerase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001653">
        <property type="entry name" value="DAP_epimerase_DapF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31689">
        <property type="entry name" value="PTHR31689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01678">
        <property type="entry name" value="DAP_epimerase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00652">
        <property type="entry name" value="DapF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01326">
        <property type="entry name" value="DAP_EPIMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 8 10" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6 8" id="KW-0457">Lysine biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Substrate binding" evidence="8">
        <location>
            <begin position="77"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="8">
        <location>
            <begin position="228"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="8">
        <location>
            <begin position="238"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="active site" evidence="9">
        <location>
            <position position="76"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="8">
        <location>
            <position position="76"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="8">
        <location>
            <position position="237"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="11"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="67"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="171"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="210"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Could be important to modulate the pK values of the two catalytic cysteine residues" evidence="8">
        <location>
            <position position="173"/>
        </location>
    </feature>
    <feature type="site" description="Could be important to modulate the pK values of the two catalytic cysteine residues" evidence="8">
        <location>
            <position position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00197"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AEF95645.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="295" mass="33384" checksum="4C6E959C8E1382F1" modified="2011-07-27" version="1">MEFTKMHALGNDYIVINEFDGEVVKEEEKSEFSKKICRRGFSVGADGVIFIQKPTSDEFDVRFRIFNSDGSEAEMCGNGIRCFSKYVYERILKKNPLRVETKGGLRISEMEIENNEVKKIKVYMGVPKFKLKDIPMKIEGYDENDEFINGEIELNNPYLKKVKLSVVNVGNPHAVIFVEDNGIDLDFARKHLDVIGKEIEYHKVFPERINVHFVEVLNGNEIRILTWERGAGYTTACGTGTTASVIIANKLGKTKNKVLAHLDGGDLEIEVKNDGVYMIGDAVMVYDGKLLNIGW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-09-29" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEM3</accession>
    <name>F6BEM3_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="3">AAA ATPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_1283</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the archaeal ATPase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96820.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799417.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96820">
        <property type="protein sequence ID" value="AEF96820"/>
        <property type="gene ID" value="Metig_1283"/>
    </dbReference>
    <dbReference type="GeneID" id="10644146"/>
    <dbReference type="KEGG" id="mig:Metig_1283"/>
    <dbReference type="HOGENOM" id="CLU_068608_0_0_2"/>
    <dbReference type="OMA" id="YINLRAT"/>
    <dbReference type="OrthoDB" id="57866at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011579">
        <property type="entry name" value="ATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01637">
        <property type="entry name" value="ATPase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA" evidence="2">
        <location>
            <begin position="21"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF96820.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="351" mass="41364" checksum="E022A4869F03A055" modified="2011-07-27" version="1">MKFFNREKEIKEVLSIIESEPNFIYFIYGPINSGKTALINEVINNLDKDKYVVFYINLRRYFISKYDDFIEVLFEEYEEDKNPIELVKSLIKDSSVIYGIPIPKNTLNTLLDKKRPKNIFKYITDILLKIEKEGKRPIIIMDELQKIGDLEINGFLIYELFNYFISLTKEMHLSHVFCLSSDSLFIEKVYNGAMLKGRTDYILVDDFDKETALKFMGFLAGDVLNRALSEEEKEKIYSYVGGKPVLIIKVMNRMKTDDLDEILNYLLNMEISELMDLLAKVKNNKFEGIEYNKVVNALKLFKNSYEIDAYSIKEDIKEFLIKRNILFLEPVKRILRPQSFLTWRAIKILLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEN1</accession>
    <name>F6BEN1_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">50S ribosomal protein L18Ae</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">50S ribosomal protein L20e</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="3">50S ribosomal protein LX</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">rpl18a</name>
        <name evidence="3" type="synonym">rpl20e</name>
        <name evidence="3" type="synonym">rplX</name>
        <name evidence="5" type="ordered locus">Metig_1291</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="3">Part of the 50S ribosomal subunit. Binds 23S rRNA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the eukaryotic ribosomal protein eL20 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96828.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799425.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1291"/>
    <dbReference type="EnsemblBacteria" id="AEF96828">
        <property type="protein sequence ID" value="AEF96828"/>
        <property type="gene ID" value="Metig_1291"/>
    </dbReference>
    <dbReference type="GeneID" id="10644154"/>
    <dbReference type="KEGG" id="mig:Metig_1291"/>
    <dbReference type="HOGENOM" id="CLU_177460_0_1_2"/>
    <dbReference type="OMA" id="MVFTKEY"/>
    <dbReference type="OrthoDB" id="125208at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070180">
        <property type="term" value="F:large ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00273">
        <property type="entry name" value="Ribosomal_L18Ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028877">
        <property type="entry name" value="50S_L18Ae/Ribosomal_L18a/L20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023573">
        <property type="entry name" value="Ribosomal_L18a//L18Ae/LX"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01775">
        <property type="entry name" value="Ribosomal_L18A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="1 3 5" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="Ribosomal_L18A" evidence="4">
        <location>
            <begin position="2"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEF96828.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="76" mass="9079" checksum="2FBE336208613EB6" modified="2011-07-27" version="1">MVKIFRIKGEVLGKDEPMVFTKEYRALKEEHALELMYSEIGSKHRVRRRMIKVHEVKEISEDEVTDPILKKMLAMY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEP1</accession>
    <name>F6BEP1_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcriptional regulator, ArsR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_1301</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96838.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799435.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1301"/>
    <dbReference type="EnsemblBacteria" id="AEF96838">
        <property type="protein sequence ID" value="AEF96838"/>
        <property type="gene ID" value="Metig_1301"/>
    </dbReference>
    <dbReference type="GeneID" id="10644164"/>
    <dbReference type="KEGG" id="mig:Metig_1301"/>
    <dbReference type="HOGENOM" id="CLU_718896_0_0_2"/>
    <dbReference type="OMA" id="SHKDINT"/>
    <dbReference type="OrthoDB" id="48466at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001845">
        <property type="entry name" value="HTH_ArsR_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="SMART" id="SM00418">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50987">
        <property type="entry name" value="HTH_ARSR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="198"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="domain" description="HTH arsR-type" evidence="2">
        <location>
            <begin position="234"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF96838.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="385" mass="44648" checksum="6C8D6BDA169C5CF1" modified="2011-07-27" version="1">MKLIIIFIYLILLLSNIMSIYGIDKFYDIREITKMSYNTTLFLEYDKEHNPIFYVNTPKDYYIAPVKINVIGVNRSLLNDMGFILDKDDYDVLISNRTVETNKNAILFIPINGSTNLNRETATLINWKIDPLNYKSSVVIAKYKLPKKGEIIAVYSDGSPACIKLNNKIYCGFKPNKEVLYNLIYIFMIKNAKNIVNIPYGLIFGTLFLGIISYLSITSQRIKEIILKSGAYLIIITGRVSINNKEKVLLNDTRKEIYNYIVNNPGVHLREIAKNLNKSVSTIIWHLRILEKANLIRSKKVGNKRIYYPKGMDVRDLQLIYLNNKLSKKIYEYLLKNPAHLRKISRDLDIPVETVRYNLRKMEELGIVDSYEEGNKIIYTVNSKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEQ8</accession>
    <name>F6BEQ8_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Asp/Glu amidotransferase, subunit C</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_1318</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96855.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799452.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1318"/>
    <dbReference type="EnsemblBacteria" id="AEF96855">
        <property type="protein sequence ID" value="AEF96855"/>
        <property type="gene ID" value="Metig_1318"/>
    </dbReference>
    <dbReference type="GeneID" id="10644181"/>
    <dbReference type="KEGG" id="mig:Metig_1318"/>
    <dbReference type="HOGENOM" id="CLU_196465_0_0_2"/>
    <dbReference type="OMA" id="MEESYYI"/>
    <dbReference type="OrthoDB" id="117523at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006450">
        <property type="term" value="P:regulation of translational fidelity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003837">
        <property type="entry name" value="Asp/Glu-ADT_csu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036113">
        <property type="entry name" value="Asp/Glu-ADT_sf_sub_c"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010120">
        <property type="entry name" value="Glu-ADT_subunit_C_archaea"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02686">
        <property type="entry name" value="Glu-tRNAGln"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141000">
        <property type="entry name" value="SSF141000"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01827">
        <property type="entry name" value="gatC_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF96855.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="79" mass="9245" checksum="D1DC672F3B02B9E7" modified="2011-07-27" version="1">MVDIEKIKKEADEIIKEFSKVLESFELDEEETYYILDTKNVLRTDDEPSLDENFRDDVLSIAPKTKDGYVVVEKGKWSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>F6BER5</accession>
    <name>F6BER5_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Glyceraldehyde 3-phosphate phosphatase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Metig_1325</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the dephosphorylation of D,L-glyceraldehyde 3-phosphate in vitro.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the HAD-like hydrolase superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96862.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799459.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1325"/>
    <dbReference type="EnsemblBacteria" id="AEF96862">
        <property type="protein sequence ID" value="AEF96862"/>
        <property type="gene ID" value="Metig_1325"/>
    </dbReference>
    <dbReference type="GeneID" id="10644188"/>
    <dbReference type="KEGG" id="mig:Metig_1325"/>
    <dbReference type="HOGENOM" id="CLU_045011_8_3_2"/>
    <dbReference type="OMA" id="TYHNVKF"/>
    <dbReference type="OrthoDB" id="93004at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006439">
        <property type="entry name" value="HAD-SF_hydro_IA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011950">
        <property type="entry name" value="HAD-SF_hydro_IA_CTE7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041492">
        <property type="entry name" value="HAD_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13419">
        <property type="entry name" value="HAD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00413">
        <property type="entry name" value="HADHALOGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="SSF56784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02253">
        <property type="entry name" value="CTE7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01549">
        <property type="entry name" value="HAD-SF-IA-v1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01509">
        <property type="entry name" value="HAD-SF-IA-v3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00019531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEF96862.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="228" mass="26111" checksum="D1965A8F78722A04" modified="2011-07-27" version="1">MIKGIFFDLDDTLYDSSNFVDIARREAIKSMIDAGLKTTEEEAYKILQKIIKDKGSNYGKHFDDLVKAVMGYYDPKIICMGIITYHNVKFALLRPYPDTIKTLIELKKMGLKLGVITDGITIKQWEKLIRLGIHPFFDVVVTSEEYGLGKPHLEFFEFGLKKMGLKAEEVIYVGDRVDKDIKPAKELGMTTIRILKGKYKDMEGESDYTVTKLPEIVDIVKNLIEKNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEU8</accession>
    <name>F6BEU8_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Serine/threonine protein kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Metig_0123</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95684.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798293.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="SMR" id="F6BEU8"/>
    <dbReference type="STRING" id="880724.Metig_0123"/>
    <dbReference type="EnsemblBacteria" id="AEF95684">
        <property type="protein sequence ID" value="AEF95684"/>
        <property type="gene ID" value="Metig_0123"/>
    </dbReference>
    <dbReference type="GeneID" id="10642958"/>
    <dbReference type="KEGG" id="mig:Metig_0123"/>
    <dbReference type="HOGENOM" id="CLU_026763_0_0_2"/>
    <dbReference type="OMA" id="CETERYQ"/>
    <dbReference type="OrthoDB" id="10990at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006468">
        <property type="term" value="P:protein phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="SSF56112"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="4">
        <location>
            <begin position="319"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="91"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEF95684.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="667" mass="78263" checksum="D55B562116332DA7" modified="2011-07-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEV3</accession>
    <name>F6BEV3_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="6">PfkB domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Metig_0129</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the carbohydrate kinase PfkB family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95689.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798298.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0129"/>
    <dbReference type="EnsemblBacteria" id="AEF95689">
        <property type="protein sequence ID" value="AEF95689"/>
        <property type="gene ID" value="Metig_0129"/>
    </dbReference>
    <dbReference type="GeneID" id="10642964"/>
    <dbReference type="KEGG" id="mig:Metig_0129"/>
    <dbReference type="HOGENOM" id="CLU_027634_5_2_2"/>
    <dbReference type="OMA" id="SQQIARM"/>
    <dbReference type="OrthoDB" id="98664at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016773">
        <property type="term" value="F:phosphotransferase activity, alcohol group as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002173">
        <property type="entry name" value="Carboh/pur_kinase_PfkB_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011611">
        <property type="entry name" value="PfkB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002139">
        <property type="entry name" value="Ribo/fructo_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00294">
        <property type="entry name" value="PfkB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00990">
        <property type="entry name" value="RIBOKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="SSF53613"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00583">
        <property type="entry name" value="PFKB_KINASES_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00584">
        <property type="entry name" value="PFKB_KINASES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PfkB" evidence="5">
        <location>
            <begin position="11"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEF95689.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="311" mass="34978" checksum="A25D60CBC2C0C499" modified="2011-07-27" version="1">MDEDNFNSKKNKIISVGHIALDYIFNVEKFPEPNTSIQIPSARKYYGGAACNVAVGVAKLGLLSGIVSCVGYDFKNSGYERYLKNLGVDISHIYHSEEEETPKAWIFTDKDNNQITFFLWGAAKHYKELNPPLFEAEIVHLATGDPEFNAKCAEKAKKNNILVSFDPGQDLPLYDKDTMERIIKNSNFLFMNKHEFERTLKLLNTDLESLRNRVDVLVVTYGKDGSVIYTKDEEIKIPSIKAEKVVDPTGAGDSYRVGFLAGYVKGYDLEQCGLIGSCVASFVIEKKGCQTNLPSWNDVIERLKKEGYVLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-09-29" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEY3</accession>
    <name>F6BEY3_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="3">AAA ATPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_0161</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the archaeal ATPase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95719.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF95719">
        <property type="protein sequence ID" value="AEF95719"/>
        <property type="gene ID" value="Metig_0161"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0161"/>
    <dbReference type="HOGENOM" id="CLU_068608_0_0_2"/>
    <dbReference type="OMA" id="ESIKQWI"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011579">
        <property type="entry name" value="ATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01637">
        <property type="entry name" value="ATPase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA" evidence="2">
        <location>
            <begin position="38"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF95719.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="355" mass="42019" checksum="BDED3C088508B9B2" modified="2011-07-27" version="1">MILFGKTQRVLPYNGDKMKFYNRERELNYLKTYCQLEPNSILFVYGPKSSGKSAVMRRVIKELEDSDLIFFYYNLREHATPTKDEFLNIFFEKGGKKYIKNNLIIDIKVCKFGVEEGWNVDDIKLNDVFRKIKENINTVIKEGKKPVLIIDELQKLKSIYFNGNGDKSLLNELFNLFVSLTKMEHLCHVICLTSDTLFIEEIYRNSTLENTSEYYLIDWLNKDCIKNILEEEGFNEEEINYAINYLSLPYEVSQLINNKKLGLSVEESIKQWINIEKDKIRYIIRENLNKKDEILKVLSKFRDNIKIKNEDIEDELFETLKILIENEILFYDVINGVIKPTSVKKWYAIKEVLKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F6BF22</accession>
    <name>F6BF22_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">4Fe-4S ferredoxin iron-sulfur binding domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1357</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96892.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799489.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1357"/>
    <dbReference type="EnsemblBacteria" id="AEF96892">
        <property type="protein sequence ID" value="AEF96892"/>
        <property type="gene ID" value="Metig_1357"/>
    </dbReference>
    <dbReference type="GeneID" id="10644220"/>
    <dbReference type="KEGG" id="mig:Metig_1357"/>
    <dbReference type="HOGENOM" id="CLU_043374_3_3_2"/>
    <dbReference type="OMA" id="KACSKRC"/>
    <dbReference type="OrthoDB" id="105930at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13247">
        <property type="entry name" value="Fer4_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="2"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="66"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96892.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="166" mass="18356" checksum="0E5EECEABCAF5217" modified="2011-07-27" version="1">MKKIMMINDKCDNCGDCVKACMEIYGVSRISILEHDGNYLPIVCQHCSSAPCMQVCPVNAIEFRGEVVYLDGEKCIGCGLCALACPFGAIVMADKAHKCILCIDRGEQACVKACSKRCLEVVSVEDAIWDKKLRNIENFAKLTLNKKIENKDGLLSKITINARVKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>F6BF24</accession>
    <name>F6BF24_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Phosphoesterase</fullName>
            <ecNumber evidence="2">3.1.4.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metig_1359</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the metallophosphoesterase superfamily. YfcE family.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.-" evidence="2"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96894.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799491.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1359"/>
    <dbReference type="EnsemblBacteria" id="AEF96894">
        <property type="protein sequence ID" value="AEF96894"/>
        <property type="gene ID" value="Metig_1359"/>
    </dbReference>
    <dbReference type="GeneID" id="10644222"/>
    <dbReference type="KEGG" id="mig:Metig_1359"/>
    <dbReference type="HOGENOM" id="CLU_063749_3_2_2"/>
    <dbReference type="OMA" id="IVVYVYE"/>
    <dbReference type="OrthoDB" id="80065at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110165">
        <property type="term" value="C:cellular anatomical entity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00841">
        <property type="entry name" value="MPP_YfcE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024654">
        <property type="entry name" value="Calcineurin-like_PHP_lpxH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041802">
        <property type="entry name" value="MPP_YfcE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000979">
        <property type="entry name" value="Phosphodiesterase_MJ0936/Vps29"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11124">
        <property type="entry name" value="PTHR11124"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12850">
        <property type="entry name" value="Metallophos_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="SSF56300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00040">
        <property type="entry name" value="yfcE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metallophos_2" evidence="3">
        <location>
            <begin position="1"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU362039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF12850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEF96894.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="158" mass="17519" checksum="9AFDB3E4D3685C39" modified="2011-07-27" version="1">MLIGIISDTHVPDRADTLPKKVVDEFSNVDLIVHCGDVTSPQVLNELKDLSKVVAVKGNMDYLELPRKEILDINDIKIGVIHGDIIYPRGDTLKLKYLGLEMGVDILISGHTHTPLIEKQKDILLLNPGSPTTPRCPIKSIMKLEIEDKKVNANLIPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>F6BF51</accession>
    <name>F6BF51_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Probable tRNA pseudouridine synthase B</fullName>
            <ecNumber evidence="2">5.4.99.25</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">tRNA pseudouridine(55) synthase</fullName>
            <shortName evidence="2">Psi55 synthase</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="2">tRNA pseudouridylate synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="2">tRNA-uridine isomerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2" type="primary">truB</name>
        <name evidence="5" type="ordered locus">Metig_1386</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Could be responsible for synthesis of pseudouridine from uracil-55 in the psi GC loop of transfer RNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>uridine(55) in tRNA = pseudouridine(55) in tRNA</text>
            <dbReference type="Rhea" id="RHEA:42532"/>
            <dbReference type="Rhea" id="RHEA-COMP:10101"/>
            <dbReference type="Rhea" id="RHEA-COMP:10102"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="EC" id="5.4.99.25"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the pseudouridine synthase TruB family. Type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.4.99.25" evidence="2"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96921.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1386"/>
    <dbReference type="EnsemblBacteria" id="AEF96921">
        <property type="protein sequence ID" value="AEF96921"/>
        <property type="gene ID" value="Metig_1386"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_1386"/>
    <dbReference type="HOGENOM" id="CLU_032087_3_0_2"/>
    <dbReference type="OMA" id="GPFKEDE"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106029">
        <property type="term" value="F:tRNA pseudouridine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031119">
        <property type="term" value="P:tRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.130.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01081">
        <property type="entry name" value="TruB_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012960">
        <property type="entry name" value="Dyskerin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002501">
        <property type="entry name" value="PsdUridine_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002478">
        <property type="entry name" value="PUA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036974">
        <property type="entry name" value="PUA_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004802">
        <property type="entry name" value="tRNA_PsdUridine_synth_B_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026326">
        <property type="entry name" value="TruB_arch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032819">
        <property type="entry name" value="TruB_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004521">
        <property type="entry name" value="Uncharacterised_CHP00451"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23127">
        <property type="entry name" value="PTHR23127"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08068">
        <property type="entry name" value="DKCLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01472">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16198">
        <property type="entry name" value="TruB_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01509">
        <property type="entry name" value="TruB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01136">
        <property type="entry name" value="DKCLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00359">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="SSF55120"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="SSF88697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00425">
        <property type="entry name" value="CBF5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00451">
        <property type="entry name" value="unchar_dom_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 2" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="DKCLD" evidence="4">
        <location>
            <begin position="17"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="domain" description="PUA" evidence="3">
        <location>
            <begin position="251"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="2">
        <location>
            <position position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM01136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEF96921.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="338" mass="38512" checksum="790ACBB65D65F7B9" modified="2011-07-27" version="1">MTKIENVQNYPNFSSEKEELIIKEEAETNEEYGCNPYERKIEDLLKSGVVVIDKPSGPTSHEVSTWVKKILNLKKAGHGGTLDPKVTGVLPIALENATKCIPMWHIPPKEYICLMHLHRDADEEKIREIFKEFEGKILQRPPLKAAVKRSLRIRKIHKLEILDIDGRDILFRVKCQSGTYIRKLVDDMGEALGTSAHMQELRRVKSGVFEENDAVYLQDLVDAYHFWKEDGDEEELRRIIKPIEYGLRHLKKVVVKDSAVDAICHGADVYVKGISKLSKGIGKGEIVLVETLKGEAIGVGKALMNTKEILKANEGVAVDVERIYMEPNTYPRMWKRKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F6BF71</accession>
    <name>F6BF71_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">AcnX domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1406</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96941.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799537.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1406"/>
    <dbReference type="EnsemblBacteria" id="AEF96941">
        <property type="protein sequence ID" value="AEF96941"/>
        <property type="gene ID" value="Metig_1406"/>
    </dbReference>
    <dbReference type="GeneID" id="10644279"/>
    <dbReference type="KEGG" id="mig:Metig_1406"/>
    <dbReference type="HOGENOM" id="CLU_018825_1_0_2"/>
    <dbReference type="OMA" id="CTCTPYL"/>
    <dbReference type="OrthoDB" id="31127at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd01355">
        <property type="entry name" value="AcnX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007506">
        <property type="entry name" value="AcnX_catalytic_put"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04412">
        <property type="entry name" value="AcnX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AcnX" evidence="1">
        <location>
            <begin position="1"/>
            <end position="405"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96941.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="407" mass="45211" checksum="A4008845E766D1F3" modified="2011-07-27" version="1">MYLTKEEEKILDGEYGEILRRCMNLLVSLGDIYGAERLIPITSSQISGVSYKTIKDVGLEFLEDFAKENVKVKVHSTLNPAGMDLDKWKELGIKEEFAEKQLRIIDAFKKMGVEISCTCTPYLIGNLPKFGEHISWAESSAVSFANSVLGAKTNREGGPSALASAILGKTPYYGYHLDENRKAEVMIKLDEELLNLLKSLKFGESFYGALGYLIGKMVKNGVPYFENLYKLNPSNDNLKSLGASMAASGGVALYHAKELTAECKVKEVVDDKIEKISIGIEEIKESYEKLNTTNDKPDLICIGCPHCSLMEIKEIAEFIKKHDIKLDCDFWICTSLHTKAILDRMGHTRIIEKAGGRVVKDTCMVVAPIEEIGYKKVATNSGKAGVYLPSFCKSEVIYGDIKDILKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>F6BF75</accession>
    <name>F6BF75_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="6">ABC-type transporter, integral membrane subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Metig_1410</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96945.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799541.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1410"/>
    <dbReference type="EnsemblBacteria" id="AEF96945">
        <property type="protein sequence ID" value="AEF96945"/>
        <property type="gene ID" value="Metig_1410"/>
    </dbReference>
    <dbReference type="GeneID" id="10644283"/>
    <dbReference type="KEGG" id="mig:Metig_1410"/>
    <dbReference type="HOGENOM" id="CLU_028518_5_3_2"/>
    <dbReference type="OMA" id="AWWLTTF"/>
    <dbReference type="OrthoDB" id="63271at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="7"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="71"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="109"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="136"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="190"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="239"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="5">
        <location>
            <begin position="67"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEF96945.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="279" mass="30453" checksum="D20684F8B3B9FD94" modified="2011-07-27" version="1">MIFKNKKFFFGFLIIFSMIILGILAPVIAEDPYKTDMLKRLSPPSLEHPFGTDQLGRDIFSRVIYGARVTLLVSFAVSLLSLTIGSLIGALSGYFGGVIDDILSRITDIFLSIPEIVLNIAMVGVLSVSLNLSSSIFIVILAIVITNWVTYARITRGLVLSLKESDFVLLAKSSGANDLYILLKHILPNALLPIIVLVTLNIGNVIMTISTLGFLGLGIQPPTPEWGAMLNSGKNYIMTSPWVVVFPGLAIMLAILGFNLMGDCLRDIFEPKSRRSVYG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>F6BA67</accession>
    <name>F6BA67_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Zinc finger C2H2-type domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0200</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95757.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798366.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0200"/>
    <dbReference type="EnsemblBacteria" id="AEF95757">
        <property type="protein sequence ID" value="AEF95757"/>
        <property type="gene ID" value="Metig_0200"/>
    </dbReference>
    <dbReference type="GeneID" id="10643035"/>
    <dbReference type="KEGG" id="mig:Metig_0200"/>
    <dbReference type="HOGENOM" id="CLU_203610_0_0_2"/>
    <dbReference type="OrthoDB" id="127812at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="SSF57667"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2H2-type" evidence="1">
        <location>
            <begin position="18"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF95757.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="49" mass="5859" checksum="497703541AD38470" modified="2011-07-27" version="1">MVKLEAIKILDRDGEVMFRCPRCGKLFRKSKDYTKHVNKAHRHLFAKEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>F6BA72</accession>
    <name>F6BA72_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized conserved protein UCP006600</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_0205</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95762.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798371.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0205"/>
    <dbReference type="EnsemblBacteria" id="AEF95762">
        <property type="protein sequence ID" value="AEF95762"/>
        <property type="gene ID" value="Metig_0205"/>
    </dbReference>
    <dbReference type="GeneID" id="10643040"/>
    <dbReference type="KEGG" id="mig:Metig_0205"/>
    <dbReference type="HOGENOM" id="CLU_142041_0_0_2"/>
    <dbReference type="OMA" id="HNDAAVN"/>
    <dbReference type="OrthoDB" id="96590at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036608">
        <property type="entry name" value="MTH777-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012033">
        <property type="entry name" value="UCP006600"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09001">
        <property type="entry name" value="DUF1890"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006600">
        <property type="entry name" value="UCP006600"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75181">
        <property type="entry name" value="SSF75181"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF95762.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="153" mass="17392" checksum="76C26615C4F143C6" modified="2011-07-27" version="1">MRVLVLLGCPEPPVLVPSFIYLTNMLKKKGCEVFVSANPAALKLVETADPEKLYIKNVGFQEIDEGLKEAFDVDYIISFVHNDAGVSYTITYMHKYKAKTIGIVFGREMKEDFVNTLKENGVEAYFVRAFHNPTPIVVTLKRILENIFENNKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAA8</accession>
    <name>F6BAA8_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glycosyl transferase group 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0241</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95798.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798407.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0241"/>
    <dbReference type="EnsemblBacteria" id="AEF95798">
        <property type="protein sequence ID" value="AEF95798"/>
        <property type="gene ID" value="Metig_0241"/>
    </dbReference>
    <dbReference type="GeneID" id="10643076"/>
    <dbReference type="KEGG" id="mig:Metig_0241"/>
    <dbReference type="HOGENOM" id="CLU_009583_2_2_2"/>
    <dbReference type="OMA" id="RFDINIS"/>
    <dbReference type="OrthoDB" id="83551at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glycos_transf_1" evidence="1">
        <location>
            <begin position="175"/>
            <end position="344"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF95798.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="367" mass="43472" checksum="908C0D9D432CC103" modified="2011-07-27" version="1">MSKILYVHNADFSKPCANRIQVLNMCRGFKKIGQDITLMSFNNDKNTLKRIYNEDIDFNVISLKPFVNYYFRSFILFLKFLSIKNKYDYIYTRDLIFAFLVSKFFKNKKVIYELHEINKRKIWIFLLKNCLNTLYSVVTVNPNNIKFFEGNHHISRIVFLPNAVDLQRFDINISKKDAREKLNLPKDKTLITYTGSLQNWKGYGTFLKSYKYLKNKENIIYLTVGGNNEQIKVLKEKYKSDNIVFIPYVENSKIPLFLKASDILVIPNSAKYEISVKYTSPLKLFEYMASRRPIIASDLPSIREIVSEEEVLFFKPDNEKDLAEKIEYLLNNKDLIEKLAKNAFEKVKNYTWEERAKRIIEVFEDEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAC5</accession>
    <name>F6BAC5_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="3">N-6 DNA methylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_0259</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95815.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798424.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0259"/>
    <dbReference type="REBASE" id="36255">
        <property type="entry name" value="Mig5ORF259P"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF95815">
        <property type="protein sequence ID" value="AEF95815"/>
        <property type="gene ID" value="Metig_0259"/>
    </dbReference>
    <dbReference type="GeneID" id="10643094"/>
    <dbReference type="KEGG" id="mig:Metig_0259"/>
    <dbReference type="HOGENOM" id="CLU_009503_0_0_2"/>
    <dbReference type="OMA" id="ENVFNIM"/>
    <dbReference type="OrthoDB" id="1239at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008170">
        <property type="term" value="F:N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006306">
        <property type="term" value="P:DNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003356">
        <property type="entry name" value="DNA_methylase_A-5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041635">
        <property type="entry name" value="Type_ISP_LLaBIII_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02384">
        <property type="entry name" value="N6_Mtase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18135">
        <property type="entry name" value="Type_ISP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N6_Mtase" evidence="1">
        <location>
            <begin position="288"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="domain" description="Type_ISP_C" evidence="2">
        <location>
            <begin position="657"/>
            <end position="996"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF18135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF95815.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="1001" mass="118631" checksum="39669E97E2B8FB39" modified="2011-07-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAC7</accession>
    <name>F6BAC7_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Metig_0261</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95817.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798426.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0261"/>
    <dbReference type="EnsemblBacteria" id="AEF95817">
        <property type="protein sequence ID" value="AEF95817"/>
        <property type="gene ID" value="Metig_0261"/>
    </dbReference>
    <dbReference type="GeneID" id="10643096"/>
    <dbReference type="KEGG" id="mig:Metig_0261"/>
    <dbReference type="HOGENOM" id="CLU_142825_4_0_2"/>
    <dbReference type="OMA" id="MRRDELY"/>
    <dbReference type="OrthoDB" id="112390at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110001">
        <property type="term" value="C:toxin-antitoxin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004540">
        <property type="term" value="F:ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008201">
        <property type="entry name" value="HepT-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01934">
        <property type="entry name" value="DUF86"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-1277">Toxin-antitoxin system</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEF95817.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="95" mass="11571" checksum="38E2CF8963943902" modified="2011-07-27" version="1">MSKRDVKAFLWDILKSIEKIEKWTKNVEFDEFVENELLQDAIIRNLEIIGEASKYIPENIRDKYSQIPWKRIIGFRNILIHKYFGIDYETTWVYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAC9</accession>
    <name>F6BAC9_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="3">CBS domain containing membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_0263</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95819.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798428.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0263"/>
    <dbReference type="EnsemblBacteria" id="AEF95819">
        <property type="protein sequence ID" value="AEF95819"/>
        <property type="gene ID" value="Metig_0263"/>
    </dbReference>
    <dbReference type="GeneID" id="10643098"/>
    <dbReference type="KEGG" id="mig:Metig_0263"/>
    <dbReference type="HOGENOM" id="CLU_040681_9_2_2"/>
    <dbReference type="OMA" id="EDVVFAF"/>
    <dbReference type="OrthoDB" id="104239at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="SSF54631"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0129">CBS domain</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBS" evidence="2">
        <location>
            <begin position="13"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="2">
        <location>
            <begin position="78"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF95819.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="140" mass="15614" checksum="04CA8EBBC8BEE5E7" modified="2011-07-27" version="1">MEDLKNIKVKDVMTTDVIYASPEDNVIEAFEILLKNKISCLPVVDKNKKVVGIMTTTDIGYNLIIDKYTLETTVGDVMTKDVVTITPEESIVDAIKKMDVYGDSKEIINQLPVVDKEGRLVGIISDGDIIRILSKILKEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAD3</accession>
    <name>F6BAD3_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="5">CoB/CoM heterodisulfide reductase, subunit C</fullName>
            <ecNumber evidence="5">1.8.98.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Metig_0267</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Cofactor metabolism; coenzyme M-coenzyme B heterodisulfide reduction; coenzyme B and coenzyme M from coenzyme M-coenzyme B heterodisulfide: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the HdrC family.</text>
    </comment>
    <dbReference type="EC" id="1.8.98.1" evidence="5"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95823.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0267"/>
    <dbReference type="EnsemblBacteria" id="AEF95823">
        <property type="protein sequence ID" value="AEF95823"/>
        <property type="gene ID" value="Metig_0267"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0267"/>
    <dbReference type="HOGENOM" id="CLU_121273_0_0_2"/>
    <dbReference type="OMA" id="AQKGNMA"/>
    <dbReference type="UniPathway" id="UPA00647">
        <property type="reaction ID" value="UER00700"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051912">
        <property type="term" value="F:CoB--CoM heterodisulfide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015948">
        <property type="term" value="P:methanogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1060.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017680">
        <property type="entry name" value="CoB/CoM_hetero-S_Rdtase_csu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009051">
        <property type="entry name" value="Helical_ferredxn"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13183">
        <property type="entry name" value="Fer4_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03290">
        <property type="entry name" value="CoB_CoM_SS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0484">Methanogenesis</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="4">
        <location>
            <begin position="25"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEF95823.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="184" mass="20766" checksum="646AA4E09E8770BC" modified="2011-07-27" version="1">MVIKMEDVNKNFVNEVIETGKLVPGEEIVKSIKACYQCGTCTGSCPSGRRTAYRTRKVLRQVLLGLDSVLESDDIWRCTTCYTCYERCPRDVKVTEIIKTVRNLAAKKGHMAPAHKKTASLVLRFGHAVPANKDTAELRKSIGLAEKAPIAQFSDKDLEEIRTLVKELEFDKLIGFNWEKMDLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAD4</accession>
    <name>F6BAD4_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Radical SAM domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metig_0268</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95824.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798433.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0268"/>
    <dbReference type="EnsemblBacteria" id="AEF95824">
        <property type="protein sequence ID" value="AEF95824"/>
        <property type="gene ID" value="Metig_0268"/>
    </dbReference>
    <dbReference type="GeneID" id="10643103"/>
    <dbReference type="KEGG" id="mig:Metig_0268"/>
    <dbReference type="HOGENOM" id="CLU_600805_0_0_2"/>
    <dbReference type="OMA" id="THRGCLG"/>
    <dbReference type="OrthoDB" id="90547at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031419">
        <property type="term" value="F:cobalamin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.30.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023979">
        <property type="entry name" value="CHP04014_B12-bd/rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006158">
        <property type="entry name" value="Cobalamin-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaB/NifB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020612">
        <property type="entry name" value="Methylthiotransferase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023404">
        <property type="entry name" value="rSAM_horseshoe"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02310">
        <property type="entry name" value="B12-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01217">
        <property type="entry name" value="B12-binding_methylthiotransfer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR04014">
        <property type="entry name" value="B12_SAM_MJ_0865"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01278">
        <property type="entry name" value="MTTASE_RADICAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="2" id="KW-0408">Iron</keyword>
    <keyword evidence="2" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Radical SAM core" evidence="3">
        <location>
            <begin position="152"/>
            <end position="381"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEF95824.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="435" mass="49401" checksum="9660E6BAB7E2BA48" modified="2011-07-27" version="1">MRITIYSPEVYTYGSMVVGGILSKKHNVHLIRKPNNALFLKSDVVILSLYSTLQIIDENIKNIVNFIRSSKRKTKVYVAGCVSSYPEIILNELNVDGVIVGEGELTTPNIVEGDTEGLAYWDGNEIVVNYPKEKPDLNHPMPLIPKDIANQNIRGANVYIETHRGCLGNCTFCQVPKFFGKEIRSRDVDLVVEEVKEFKKRGVKRIAISGGTGSLYNFKKSVNRDKFYELLEKISSIVGKNNLSVPDMRVDYVDEDILGAIKNYTIGWVFYGIESGSDKILRDMKKGVSTKKIMDAIKLAKDCDVKVGGSFIVGYPTETEMDYLLTKDFIVDAELDDIFVSIAEPIPTTELCKLVLNTPKEENPTFMRHEGEYRHLNLTESEARCFDLLIHAEMWKSSPRIMTKQLYAIYLNEAKTQGKDIRKITELIFKYKNYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAF2</accession>
    <name>F6BAF2_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Pyruvate/ketoisovalerate oxidoreductase, gamma subunit</fullName>
            <ecNumber evidence="3">1.2.7.3</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_1430</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.2.7.3" evidence="3"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96965.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799561.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1430"/>
    <dbReference type="EnsemblBacteria" id="AEF96965">
        <property type="protein sequence ID" value="AEF96965"/>
        <property type="gene ID" value="Metig_1430"/>
    </dbReference>
    <dbReference type="GeneID" id="10644303"/>
    <dbReference type="KEGG" id="mig:Metig_1430"/>
    <dbReference type="HOGENOM" id="CLU_087284_0_0_2"/>
    <dbReference type="OMA" id="EVSWMPS"/>
    <dbReference type="OrthoDB" id="76505at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047553">
        <property type="term" value="F:2-oxoglutarate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.920.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011894">
        <property type="entry name" value="PorC_KorC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019752">
        <property type="entry name" value="Pyrv/ketoisovalerate_OxRed_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002869">
        <property type="entry name" value="Pyrv_flavodox_OxRed_cen"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01558">
        <property type="entry name" value="POR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53323">
        <property type="entry name" value="SSF53323"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02175">
        <property type="entry name" value="PorC_KorC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="POR" evidence="2">
        <location>
            <begin position="11"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF96965.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="187" mass="20156" checksum="5EB8565298E39D92" modified="2011-07-27" version="1">MRKEIRLSGFGGQGIILAGVILGRAASLYDGKEAVQTQSYGPEARGGASKSEVVIDDKPIDFPKVIKPDILVCMSQPAFDKYVNDVKENGFLLIDEDLVSAVDVPNVNLYKIPFTRIANEEVGLGIVANIVMLGALTRITGIVSKESMEKAILDSVPKGTEEKNLLAFNKGYEYAGKFNGKTKGFAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAJ8</accession>
    <name>F6BAJ8_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized conserved protein UCP019262</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_1477</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97011.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799607.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1477"/>
    <dbReference type="EnsemblBacteria" id="AEF97011">
        <property type="protein sequence ID" value="AEF97011"/>
        <property type="gene ID" value="Metig_1477"/>
    </dbReference>
    <dbReference type="GeneID" id="10644350"/>
    <dbReference type="KEGG" id="mig:Metig_1477"/>
    <dbReference type="HOGENOM" id="CLU_078138_0_0_2"/>
    <dbReference type="OMA" id="SKRRRMY"/>
    <dbReference type="OrthoDB" id="66534at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016754">
        <property type="entry name" value="UCP019262"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09894">
        <property type="entry name" value="DUF2121"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF019262">
        <property type="entry name" value="UCP019262"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF97011.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="292" mass="33636" checksum="D729FDFC587C7FDF" modified="2011-07-27" version="1">MSLIIGYYGRNGAVLGGDKRNIMFRGDIEKRKKLEEMLYNGEIKDDEELLKKAEEFGIKLHIEDNRKKVRKIGDDVLVGEVKSIGIDSERRRVYLTKGKCIIVDVFNDEIRNKWTKTGSGVVVFGNRYVKKIAEEEFRKAGYRLTMMSLEEIRDLIENIFKKCNTPSVSKGYDIIYTTKECKNLEEVVKKDLDALFEYRETLRKKMIDFGKVMTIVNKIVKNGEIGVIKNGKLVLYDKYIAIDKICPNPKMFNEIEVKGDVEDGDVIVIENGEMKVKGKNTPVFVDYTICEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAK7</accession>
    <name>F6BAK7_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1486</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97020.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799615.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1486"/>
    <dbReference type="EnsemblBacteria" id="AEF97020">
        <property type="protein sequence ID" value="AEF97020"/>
        <property type="gene ID" value="Metig_1486"/>
    </dbReference>
    <dbReference type="GeneID" id="10644359"/>
    <dbReference type="KEGG" id="mig:Metig_1486"/>
    <dbReference type="HOGENOM" id="CLU_099320_0_1_2"/>
    <dbReference type="OMA" id="IMFFALI"/>
    <dbReference type="OrthoDB" id="120839at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002798">
        <property type="entry name" value="SpoIIM-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01944">
        <property type="entry name" value="SpoIIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="72"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="98"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="135"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="177"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF97020.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="205" mass="23204" checksum="80566FD7A11286CB" modified="2011-07-27" version="1">MSRAVYEILEIMEALKRHKHTIGFTAFVFVSAFILSYILIHQSEYLRYFGELMYSNFRDKVSSLGISKETPSLVLILVILSNNISVAIINYIGMIMSLAILIVNAFLLAYVLYISNPLDFFLLIAPHGIFEIPALILSASSGLVLFRGILCLIISTIKIKPLKKHFEYKREDLKDSLRIFLVSILLFVIAAVIEGTITKLISNWV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAR9</accession>
    <name>F6BAR9_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Polyamine-transporting ATPase</fullName>
            <ecNumber evidence="9">3.6.3.31</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Metig_0327</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="3.6.3.31" evidence="9"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95883.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798492.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0327"/>
    <dbReference type="EnsemblBacteria" id="AEF95883">
        <property type="protein sequence ID" value="AEF95883"/>
        <property type="gene ID" value="Metig_0327"/>
    </dbReference>
    <dbReference type="GeneID" id="10643163"/>
    <dbReference type="KEGG" id="mig:Metig_0327"/>
    <dbReference type="HOGENOM" id="CLU_000604_1_1_2"/>
    <dbReference type="OMA" id="RPENFHE"/>
    <dbReference type="OrthoDB" id="88924at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015408">
        <property type="term" value="F:ABC-type ferric iron transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03259">
        <property type="entry name" value="ABC_Carb_Solutes_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015853">
        <property type="entry name" value="ABC_transpr_FbpC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008995">
        <property type="entry name" value="Mo/tungstate-bd_C_term_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013611">
        <property type="entry name" value="Transp-assoc_OB_typ2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08402">
        <property type="entry name" value="TOBE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50331">
        <property type="entry name" value="SSF50331"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="8">
        <location>
            <begin position="2"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="34"/>
            <end position="41"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AEF95883.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="384" mass="43179" checksum="46EC0C16ED3C9902" modified="2011-07-27" version="1">MIEINNISKRIDGQEILNDVSFSVNDSEIMVLLGPSGCGKTTLLKIIAGLLKQDYGDVVINGKVINDLPPQKRNVGFVFQDYALFPHKNVFENIAFGLKIRKIPENEIKKRVYEILEILEITHLKDKKIPQLSGGQKQRVALARALVINPDVLLLDEPLSALDPVLREKLREELKAILKNLGVTGIYVTHDLTEAMLLGDSVAVMNKGEIQQIGKPDEIFYHPKNEFVAKFVGVKNILKGNIKELKDDIATVEVVNEGLKSPFTIKVKKYPIFERKHNNNISLCIHPEDIILDKTKKYENAIKGKIINIIPNGSVLKVVVDIGGLEIYAVTTRHLLKYDVNDEVWVSFSREAPHPMCGKKCKSPNQEALCKCKALKNTIEEIEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAS1</accession>
    <name>F6BAS1_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF364 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0329</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95885.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798494.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0329"/>
    <dbReference type="EnsemblBacteria" id="AEF95885">
        <property type="protein sequence ID" value="AEF95885"/>
        <property type="gene ID" value="Metig_0329"/>
    </dbReference>
    <dbReference type="GeneID" id="10643165"/>
    <dbReference type="KEGG" id="mig:Metig_0329"/>
    <dbReference type="HOGENOM" id="CLU_1136483_0_0_2"/>
    <dbReference type="OMA" id="IIGFQPA"/>
    <dbReference type="OrthoDB" id="100761at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007161">
        <property type="entry name" value="DUF364"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04016">
        <property type="entry name" value="DUF364"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF364" evidence="1">
        <location>
            <begin position="128"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF95885.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="242" mass="26876" checksum="CF74FEAEBCA6F388" modified="2011-07-27" version="1">MYDVIYEKAMETLKPILNKTVVIKTLAPKEAIGNVDREDYPIIKGKEIMIEADIEGKRGQAFTSYPINTKGTLKEFIEKIKGDERDRAIAIASINAGMNYLGLLDRCVHCKDDKPKECGKRLALKILREFGGDAIVAHIGFQPGHIEATSKLFKVYITDLNPENIGKEKFGTVVLDGKECNDKIIKKSDIALITGSTIVNGTFWELLKTCEKYDTIPIIYGVTVQGVAKLLGMDVYCPFAGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAU2</accession>
    <name>F6BAU2_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="6">ABC-type transporter, integral membrane subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Metig_0350</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95906.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798515.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0350"/>
    <dbReference type="EnsemblBacteria" id="AEF95906">
        <property type="protein sequence ID" value="AEF95906"/>
        <property type="gene ID" value="Metig_0350"/>
    </dbReference>
    <dbReference type="GeneID" id="10643187"/>
    <dbReference type="KEGG" id="mig:Metig_0350"/>
    <dbReference type="HOGENOM" id="CLU_046113_1_4_2"/>
    <dbReference type="OMA" id="LIEFYRP"/>
    <dbReference type="OrthoDB" id="68860at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="7"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="36"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="66"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="107"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="175"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="214"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="5">
        <location>
            <begin position="57"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEF95906.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="253" mass="27612" checksum="C35BFB89A2F4321C" modified="2011-07-27" version="1">MKLLRNLILPVLGVVLWEVFAIYLNNPVIIPKVESVLNVLLNPWTGILGTGNLIDNTLISIKRVISGFIVAALVAIPLGILMGYYSFVNDLFDTVIELLRPIPPLAWVPLALAWFGIGETSMLFIIFIGAFFPILINTISGVKGVPKPLIEAALTLGAKEKDILMKVVIPASSPSILTGLRVGAGIAWMCVVAAEMLPGSDAGLGYLIMYAYSLSRMDVVIAAMVIIGLIGLILDRGLRFIENRYFKWRTMMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAW4</accession>
    <name>F6BAW4_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">PKD domain containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1517</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97051.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799645.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1517"/>
    <dbReference type="EnsemblBacteria" id="AEF97051">
        <property type="protein sequence ID" value="AEF97051"/>
        <property type="gene ID" value="Metig_1517"/>
    </dbReference>
    <dbReference type="GeneID" id="10644390"/>
    <dbReference type="KEGG" id="mig:Metig_1517"/>
    <dbReference type="HOGENOM" id="CLU_278826_0_0_2"/>
    <dbReference type="OMA" id="NTTIHLE"/>
    <dbReference type="OrthoDB" id="5767at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007253">
        <property type="entry name" value="Cell_wall-bd_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022409">
        <property type="entry name" value="PKD/Chitinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000601">
        <property type="entry name" value="PKD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035986">
        <property type="entry name" value="PKD_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04122">
        <property type="entry name" value="CW_binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00089">
        <property type="entry name" value="PKD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49299">
        <property type="entry name" value="SSF49299"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50093">
        <property type="entry name" value="PKD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PKD" evidence="1">
        <location>
            <begin position="730"/>
            <end position="800"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF97051.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="1132" mass="122734" checksum="6D219D441443D045" modified="2011-07-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAX9</accession>
    <name>F6BAX9_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0210 protein Metig_1532</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1532</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0210 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97066.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799660.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1532"/>
    <dbReference type="EnsemblBacteria" id="AEF97066">
        <property type="protein sequence ID" value="AEF97066"/>
        <property type="gene ID" value="Metig_1532"/>
    </dbReference>
    <dbReference type="GeneID" id="10644405"/>
    <dbReference type="KEGG" id="mig:Metig_1532"/>
    <dbReference type="HOGENOM" id="CLU_048704_0_0_2"/>
    <dbReference type="OMA" id="EMAIGMV"/>
    <dbReference type="OrthoDB" id="26259at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd08025">
        <property type="entry name" value="RNR_PFL_like_DUF711"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01221">
        <property type="entry name" value="UPF0210"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007841">
        <property type="entry name" value="UPF0210"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37560">
        <property type="entry name" value="PTHR37560"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05167">
        <property type="entry name" value="DUF711"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF97066.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="458" mass="48446" checksum="BD7F43F14BCEA7C9" modified="2011-07-27" version="1">MFLPEEIIETIRMIEYENLDVRTTTLGINLKDCISEDLDTLKENIYNKITTIAENLVEVANRVSEKYGIPIVNKRIAVTPISLIIGSAIRDLSREEKINACVEIGKTLDKAAKKVRVDFIGGYSALVHKGATKEDKALIDSIPYMMEKTEKVCSSVNVATTKSGINMNAVKRMGEIIKETSLRTENAIGCAKLVVFANAPEDNPFMAGAFHGVGEGDAVLNVGVSGPGVVRAVVEKMPDADLGTLSNEIKKTAFKITRVGELVGREVAKELGVEFGIVDLSLAPTPARGDSIANILEAMGLEKCGAHGTTAALALLNDAVKKGGAMATSYVGGLSGAFIPVSEDAGMVEAVEAGALRIEKLEAMTCVCSVGLDMIAIPGKTPASTIAAIIADEMAIGVINKKTTAVRIIPVPGKDVGDYVDFGGLLGKAPIMPVNEFSSEKFINRGGRIPAPIQSLTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAY5</accession>
    <name>F6BAY5_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Tryptophan synthase alpha chain</fullName>
            <ecNumber evidence="8">4.2.1.20</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">trpA</name>
        <name evidence="10" type="ordered locus">Metig_1538</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 8">The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>(1S,2R)-1-C-(indol-3-yl)glycerol 3-phosphate + L-serine = D-glyceraldehyde 3-phosphate + H2O + L-tryptophan</text>
            <dbReference type="Rhea" id="RHEA:10532"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:57912"/>
            <dbReference type="ChEBI" id="CHEBI:58866"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="4.2.1.20"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 8">Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 5/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Tetramer of two alpha and two beta chains.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8 9">Belongs to the TrpA family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.20" evidence="8"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97072.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799666.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1538"/>
    <dbReference type="EnsemblBacteria" id="AEF97072">
        <property type="protein sequence ID" value="AEF97072"/>
        <property type="gene ID" value="Metig_1538"/>
    </dbReference>
    <dbReference type="GeneID" id="10644411"/>
    <dbReference type="KEGG" id="mig:Metig_1538"/>
    <dbReference type="HOGENOM" id="CLU_016734_0_2_2"/>
    <dbReference type="OMA" id="LVMTYWN"/>
    <dbReference type="OrthoDB" id="65368at2157"/>
    <dbReference type="UniPathway" id="UPA00035">
        <property type="reaction ID" value="UER00044"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004834">
        <property type="term" value="F:tryptophan synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04724">
        <property type="entry name" value="Tryptophan_synthase_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00131">
        <property type="entry name" value="Trp_synth_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018204">
        <property type="entry name" value="Trp_synthase_alpha_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002028">
        <property type="entry name" value="Trp_synthase_suA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43406">
        <property type="entry name" value="PTHR43406"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00290">
        <property type="entry name" value="Trp_syntA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="SSF51366"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00262">
        <property type="entry name" value="trpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00167">
        <property type="entry name" value="TRP_SYNTHASE_ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="6 8" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="7 8 10" id="KW-0456">Lyase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0822">Tryptophan biosynthesis</keyword>
    <feature type="active site" description="Proton acceptor" evidence="8">
        <location>
            <position position="48"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="8">
        <location>
            <position position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AEF97072.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="284" mass="31934" checksum="1476A98C866D4C11" modified="2011-07-27" version="1">MSRLAEKFEELKNKNEKALVAFYVGGDPNLEISKEALEVIANIADIVEIGIPFSDPVADGPTIQKADVRALNKGMNPLKAFELVKELDEKAPNTPKVFLTYYNIIFNMGEEEFVKRCKECGIDGLVVPDLPIEEAESLYNICKKYGVDLIFLVAPTTPDERLKRILEKCSGFVYVVSVTGITGVREKVQEETKELVKRVARYSNVPICVGFGISKREHVEEITQYADGAIVGSAIVRIVEKYLDENGEIKDKEAFLRELEEFVKELKEGTKQKILVKNKVKTKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAZ1</accession>
    <name>F6BAZ1_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 5">50S ribosomal protein L30e</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">rpl30e</name>
        <name evidence="7" type="ordered locus">Metig_1544</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the eukaryotic ribosomal protein eL30 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97078.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799672.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1544"/>
    <dbReference type="EnsemblBacteria" id="AEF97078">
        <property type="protein sequence ID" value="AEF97078"/>
        <property type="gene ID" value="Metig_1544"/>
    </dbReference>
    <dbReference type="GeneID" id="10644417"/>
    <dbReference type="KEGG" id="mig:Metig_1544"/>
    <dbReference type="HOGENOM" id="CLU_130502_1_0_2"/>
    <dbReference type="OMA" id="RPHKIMA"/>
    <dbReference type="OrthoDB" id="117214at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00481">
        <property type="entry name" value="Ribosomal_L30e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039109">
        <property type="entry name" value="L30/L30e/YlxQ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029064">
        <property type="entry name" value="L30e-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000231">
        <property type="entry name" value="Ribosomal_L30e"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022991">
        <property type="entry name" value="Ribosomal_L30e_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004038">
        <property type="entry name" value="Ribosomal_L7Ae/L30e/S12e/Gad45"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11449">
        <property type="entry name" value="PTHR11449"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11449:SF1">
        <property type="entry name" value="PTHR11449:SF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01248">
        <property type="entry name" value="Ribosomal_L7Ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55315">
        <property type="entry name" value="SSF55315"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00709">
        <property type="entry name" value="RIBOSOMAL_L30E_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00993">
        <property type="entry name" value="RIBOSOMAL_L30E_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 5 7" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Ribosomal_L7Ae" evidence="6">
        <location>
            <begin position="2"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00029528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEF97078.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="105" mass="11309" checksum="4AC6E0E1BB729A94" modified="2011-07-27" version="1">MDINKAIRTAVDTGKVILGSKRTIKFIKHGEGKLVIIAGNCPKELEEDVKYYAKLSDIPVYTHNVTSLELGAICGKPFPVAALLVLEEGNSNILSLIKEGNSGDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-06-02" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>F6BB10</accession>
    <name>F6BB10_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Branched-chain amino acid transport</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1563</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97097.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799691.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF97097">
        <property type="protein sequence ID" value="AEF97097"/>
        <property type="gene ID" value="Metig_1563"/>
    </dbReference>
    <dbReference type="GeneID" id="10644436"/>
    <dbReference type="KEGG" id="mig:Metig_1563"/>
    <dbReference type="HOGENOM" id="CLU_172997_0_0_2"/>
    <dbReference type="OMA" id="WENFGIS"/>
    <dbReference type="OrthoDB" id="130327at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008407">
        <property type="entry name" value="Brnchd-chn_aa_trnsp_AzlD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05437">
        <property type="entry name" value="AzlD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="37"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="85"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF97097.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="103" mass="11707" checksum="09F9A67E5D1A277B" modified="2011-07-27" version="1">MKHYLAILVVSIGTYLMRLLPIIFNKRFKKLKGVNEFFAYSSSAIISALFVTSLVSFPIKLESLSISIIALIFVFLSYKKWENFGISILIGVIVHLILNVILR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>F6BB29</accession>
    <name>F6BB29_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Nickel-dependent hydrogenase large subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_0358</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95914.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798523.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0358"/>
    <dbReference type="EnsemblBacteria" id="AEF95914">
        <property type="protein sequence ID" value="AEF95914"/>
        <property type="gene ID" value="Metig_0358"/>
    </dbReference>
    <dbReference type="GeneID" id="10643195"/>
    <dbReference type="KEGG" id="mig:Metig_0358"/>
    <dbReference type="HOGENOM" id="CLU_932593_0_0_2"/>
    <dbReference type="OMA" id="CERCYYL"/>
    <dbReference type="OrthoDB" id="46044at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.645.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029014">
        <property type="entry name" value="NiFe-Hase_large"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56762">
        <property type="entry name" value="SSF56762"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF95914.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="298" mass="33980" checksum="B8A39D5EECC3029F" modified="2011-07-27" version="1">MRIRGFESSLMEKPYEFAPVALARLCCNTEVSNALASVMAIEDAFKVSVPKEAIILRKIANLGEIIEGNAIKLRPFRDTEELIEIGEKIKLIAGKKAKYIVVGGVSKNISEKRKEKLKELAKEGLNYISREFIEMVNERKKKIPLPDLNLVSANSFSIEDVEVNGFPKNALFYKRAVYSGPLSRMYEKGVINSKNTWDILIARELEMEMALKEVFELLNKLTLTYPYIEPTVNDGKGKAIVESPEGLVYHEVVIKNGRIDDYTIINGEHFNKVVLDRIEENKEEAERIFGFCERCYYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>F6BB44</accession>
    <name>F6BB44_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">UDP-N-acetylglucosamine 2-epimerase</fullName>
            <ecNumber evidence="2">5.1.3.14</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0373</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EC" id="5.1.3.14" evidence="2"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95929.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798538.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0373"/>
    <dbReference type="EnsemblBacteria" id="AEF95929">
        <property type="protein sequence ID" value="AEF95929"/>
        <property type="gene ID" value="Metig_0373"/>
    </dbReference>
    <dbReference type="GeneID" id="10643210"/>
    <dbReference type="KEGG" id="mig:Metig_0373"/>
    <dbReference type="HOGENOM" id="CLU_041674_0_1_2"/>
    <dbReference type="OMA" id="RYNTERP"/>
    <dbReference type="OrthoDB" id="18594at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008761">
        <property type="term" value="F:UDP-N-acetylglucosamine 2-epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003331">
        <property type="entry name" value="UDP_GlcNAc_Epimerase_2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029767">
        <property type="entry name" value="WecB-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43174">
        <property type="entry name" value="PTHR43174"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02350">
        <property type="entry name" value="Epimerase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00236">
        <property type="entry name" value="wecB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0413">Isomerase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Epimerase_2" evidence="1">
        <location>
            <begin position="24"/>
            <end position="361"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF95929.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="364" mass="41557" checksum="CCC95A2021A9ACC3" modified="2011-07-27" version="1">MKLSIILGTRPEIIKLSPIIRELEGRNLDWSLIHTNQHYSENMDRIFFQELNLPNPKYNLGVGSGTHGEQTGRMLIKIEKVLLKERPDVVIVQGDTNTVLAGALAASKLKIKVAHVEAGLRSYDKNMPEEINRVLTDHISDYLFAPTEKAKNNLLKEGIEENRIFVVGNTIVDATLQNLKIAEKNEHIKEFFKNISNEEGYFLLTLHRAENVDNKERLKNIIDAITKITEIYDKNIVFPIHPRTEKRLKEFNLFDKLKSNKKIKIIEPIGYLEFLILEKNAELILTDSGGVQEESCILKVPCITLRDNTERPETLEVGSNILVGDDKNKIINAVELMLNKKRDWKNPFGDGKSGKRIVEILMSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBE5</accession>
    <name>F6BBE5_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="6">NADH dehydrogenase (Ubiquinone)</fullName>
            <ecNumber evidence="6">1.6.5.3</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Metig_1619</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="1.6.5.3" evidence="6"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97152.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799745.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1619"/>
    <dbReference type="EnsemblBacteria" id="AEF97152">
        <property type="protein sequence ID" value="AEF97152"/>
        <property type="gene ID" value="Metig_1619"/>
    </dbReference>
    <dbReference type="GeneID" id="10644492"/>
    <dbReference type="KEGG" id="mig:Metig_1619"/>
    <dbReference type="HOGENOM" id="CLU_015134_0_2_2"/>
    <dbReference type="OMA" id="KIMARIQ"/>
    <dbReference type="OrthoDB" id="88886at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001694">
        <property type="entry name" value="NADH_UbQ_OxRdtase_su1/FPO"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00146">
        <property type="entry name" value="NADHdh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0830">Ubiquinone</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="67"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="127"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="165"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="218"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="245"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="271"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEF97152.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="291" mass="31588" checksum="F1E874D3BAFEE128" modified="2011-07-27" version="1">MNFDALLGSLSLSLYAFLVGSLLLGLHRKIMARIQGRPGPPIIQYLLHILKFYVKEITFPISAGNPLYVFVALMSLMVWLGALFIGISLKSSLLILIGLYVLQKIIEHGCGLSSGSPYGKVGGVRSVFSAAAEVPLFAVVGIIYLSTHSVIIEDILAYEAAHGPLLFKLPLAAFAFFVLVLSKAPYSPFTIVKGKDIVSGYITEHYGLLESIILMGEAVAWFVLLWLFLALFIGPIVVSNPIITLIAMTIMTVVISFICALTPLLAPHHSVMLQITIAALVLIDVAYRLMT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBF0</accession>
    <name>F6BBF0_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="4">NADH dehydrogenase (Quinone)</fullName>
            <ecNumber evidence="4">1.6.99.5</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metig_1624</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the complex I 49 kDa subunit family.</text>
    </comment>
    <dbReference type="EC" id="1.6.99.5" evidence="4"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97157.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799750.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1624"/>
    <dbReference type="EnsemblBacteria" id="AEF97157">
        <property type="protein sequence ID" value="AEF97157"/>
        <property type="gene ID" value="Metig_1624"/>
    </dbReference>
    <dbReference type="GeneID" id="10644497"/>
    <dbReference type="KEGG" id="mig:Metig_1624"/>
    <dbReference type="HOGENOM" id="CLU_015134_1_2_2"/>
    <dbReference type="OMA" id="TFAECIE"/>
    <dbReference type="OrthoDB" id="4328at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008901">
        <property type="term" value="F:ferredoxin hydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016151">
        <property type="term" value="F:nickel cation binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016651">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048038">
        <property type="term" value="F:quinone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.645.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001135">
        <property type="entry name" value="NADH_Q_OxRdtase_suD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014029">
        <property type="entry name" value="NADH_UbQ_OxRdtase_49kDa_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001501">
        <property type="entry name" value="Ni-dep_hyd_lsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018194">
        <property type="entry name" value="Ni-dep_hyd_lsu_Ni_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029014">
        <property type="entry name" value="NiFe-Hase_large"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00346">
        <property type="entry name" value="Complex1_49kDa"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00374">
        <property type="entry name" value="NiFeSe_Hases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56762">
        <property type="entry name" value="SSF56762"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00535">
        <property type="entry name" value="COMPLEX1_49K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00507">
        <property type="entry name" value="NI_HGENASE_L_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0520">NAD</keyword>
    <keyword evidence="1 4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-1278">Translocase</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Complex1_49kDa" evidence="3">
        <location>
            <begin position="119"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="domain" description="Complex1_49kDa" evidence="3">
        <location>
            <begin position="280"/>
            <end position="358"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEF97157.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="375" mass="42720" checksum="03370B8353663D8C" modified="2011-07-27" version="1">MAVIPIGPIHPILKEPLRIKLHVEGERVVDAEIEMGYVHRGIEKIMEGKHYLKGVHLAERVCGICSYIHTQTFVECIEHISKIDVPDKAKYLRVITCELERIHSHLIASAVYNFAIEHETIGMWLLNVREMIMDLLEMITGNRVNMGFNVVGGVRVDIDREMMDKIYRTLDKFEDDIKNIIEAFETGPMIGLRSKGIGVIGYGEIMKTRAVGPVARASGLPESDWRLRHPTYQELKFKPVWREEGDNFARMMVRHEEVLASIELVRKALELYEECSGGVRKKVHVKGGEGEWRNEAPRGEVLYKIAITNDGIIKRIMIRTPTVMNLEAYKYMLKTCPTISDAVATYASIDPCVSCTERAIIVKDVNNGKERTIKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBF1</accession>
    <name>F6BBF1_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">4Fe-4S ferredoxin iron-sulfur binding domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1625</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97158.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799751.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1625"/>
    <dbReference type="EnsemblBacteria" id="AEF97158">
        <property type="protein sequence ID" value="AEF97158"/>
        <property type="gene ID" value="Metig_1625"/>
    </dbReference>
    <dbReference type="GeneID" id="10644498"/>
    <dbReference type="KEGG" id="mig:Metig_1625"/>
    <dbReference type="HOGENOM" id="CLU_061721_0_0_2"/>
    <dbReference type="OMA" id="IWYLYEF"/>
    <dbReference type="OrthoDB" id="93710at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00037">
        <property type="entry name" value="Fer4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12838">
        <property type="entry name" value="Fer4_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="39"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="75"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="119"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="149"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="208"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="229"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="270"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="301"/>
            <end position="330"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF97158.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="382" mass="43854" checksum="2651561949D8157A" modified="2011-07-27" version="1">MSSAIWYLYEFAKKKWLKKFFDAKTEKESTIPQKRYRKVPPIVQYIEKCISCTACKESCPSFAIEMIYNKEFKKEIPKIDDGSCISCGNCIEACPTGVLEIDSLREDTDGLPFDIPKYTNLIIDEELCVKCGLCKENCPVETIHYEEGKYKIHLHDCVACNRCVEVCPVDAIRKFTEKDLKEKIDKAQKIKFKMLTGEIEIEKNKINKVPYIVESLCIDCYNCVDVCVGKIDIKNHKVIECVKCGLCLEVCPTNALRTEKLKPKPKRTDVCYMVDESKCIGCRICYNVCNVNAIKIGDETKLPYINPLKCAREGMCARECPVNAISLVETEKALMKYKLTAICDELRGIMRRDLEEYSKKYVLAKSELEKIAENEIKKLFKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBI3</accession>
    <name>F6BBI3_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Heavy metal translocating P-type ATPase</fullName>
            <ecNumber evidence="10">3.6.3.4</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Metig_0436</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.6.3.4" evidence="10"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95992.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798601.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0436"/>
    <dbReference type="EnsemblBacteria" id="AEF95992">
        <property type="protein sequence ID" value="AEF95992"/>
        <property type="gene ID" value="Metig_0436"/>
    </dbReference>
    <dbReference type="GeneID" id="10643273"/>
    <dbReference type="KEGG" id="mig:Metig_0436"/>
    <dbReference type="HOGENOM" id="CLU_001771_0_3_2"/>
    <dbReference type="OMA" id="HWMLPAW"/>
    <dbReference type="OrthoDB" id="21472at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019829">
        <property type="term" value="F:ATPase-coupled cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00371">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017969">
        <property type="entry name" value="Heavy-metal-associated_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006122">
        <property type="entry name" value="HMA_Cu_ion-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006121">
        <property type="entry name" value="HMA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036163">
        <property type="entry name" value="HMA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027256">
        <property type="entry name" value="P-typ_ATPase_IB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00403">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55008">
        <property type="entry name" value="SSF55008"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="SSF56784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="SSF81653"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="SSF81660"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="SSF81665"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01525">
        <property type="entry name" value="ATPase-IB_hvy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00003">
        <property type="entry name" value="TIGR00003"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01047">
        <property type="entry name" value="HMA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50846">
        <property type="entry name" value="HMA_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0186">Copper</keyword>
    <keyword evidence="10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-1278">Translocase</keyword>
    <keyword evidence="1 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="156"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="182"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="217"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="242"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="395"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="422"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="732"/>
            <end position="755"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="761"/>
            <end position="780"/>
        </location>
    </feature>
    <feature type="domain" description="HMA" evidence="9">
        <location>
            <begin position="2"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="domain" description="HMA" evidence="9">
        <location>
            <begin position="72"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AEF95992.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="793" mass="87375" checksum="FFF96FA86C795788" modified="2011-07-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBI9</accession>
    <name>F6BBI9_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Methyl-coenzyme M reductase subunit beta</fullName>
            <ecNumber evidence="8">2.8.4.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Coenzyme-B sulfoethylthiotransferase beta</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">Metig_0442</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Component of the methyl-coenzyme M reductase (MCR) I that catalyzes the reductive cleavage of methyl-coenzyme M (CoM-S-CH3 or 2-(methylthio)ethanesulfonate) using coenzyme B (CoB or 7-mercaptoheptanoylthreonine phosphate) as reductant which results in the production of methane and the mixed heterodisulfide of CoB and CoM (CoM-S-S-CoB). This is the final step in methanogenesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>coenzyme B + methyl-coenzyme M = coenzyme M-coenzyme B heterodisulfide + methane</text>
            <dbReference type="Rhea" id="RHEA:12532"/>
            <dbReference type="ChEBI" id="CHEBI:16183"/>
            <dbReference type="ChEBI" id="CHEBI:58286"/>
            <dbReference type="ChEBI" id="CHEBI:58411"/>
            <dbReference type="ChEBI" id="CHEBI:58596"/>
            <dbReference type="EC" id="2.8.4.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="1">
            <dbReference type="Rhea" id="RHEA:12533"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>coenzyme F430</name>
            <dbReference type="ChEBI" id="CHEBI:60540"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4 8">One-carbon metabolism; methyl-coenzyme M reduction; methane from methyl-coenzyme M: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Hexamer of two alpha, two beta, and two gamma chains.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">MCR is a hexamer of two alpha, two beta, and two gamma chains, forming a dimer of heterotrimers.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the methyl-coenzyme M reductase beta subunit family.</text>
    </comment>
    <dbReference type="EC" id="2.8.4.1" evidence="8"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95998.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798607.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0442"/>
    <dbReference type="EnsemblBacteria" id="AEF95998">
        <property type="protein sequence ID" value="AEF95998"/>
        <property type="gene ID" value="Metig_0442"/>
    </dbReference>
    <dbReference type="GeneID" id="10643279"/>
    <dbReference type="KEGG" id="mig:Metig_0442"/>
    <dbReference type="HOGENOM" id="CLU_617682_0_0_2"/>
    <dbReference type="OMA" id="WALWNAY"/>
    <dbReference type="OrthoDB" id="25508at2157"/>
    <dbReference type="UniPathway" id="UPA00646">
        <property type="reaction ID" value="UER00699"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050524">
        <property type="term" value="F:coenzyme-B sulfoethylthiotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015948">
        <property type="term" value="P:methanogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.840.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.470">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008924">
        <property type="entry name" value="Me_CoM_Rdtase_asu/bsu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015823">
        <property type="entry name" value="Me_CoM_Rdtase_asu_N_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003179">
        <property type="entry name" value="Me_CoM_Rdtase_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022679">
        <property type="entry name" value="Me_CoM_Rdtase_bsu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022680">
        <property type="entry name" value="Me_CoM_Rdtase_bsu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009024">
        <property type="entry name" value="Me_CoM_Rdtase_Fd-like_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02241">
        <property type="entry name" value="MCR_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02783">
        <property type="entry name" value="MCR_beta_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000263">
        <property type="entry name" value="Meth_CoM_rd_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48081">
        <property type="entry name" value="SSF48081"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55088">
        <property type="entry name" value="SSF55088"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03257">
        <property type="entry name" value="met_CoM_red_bet"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0484">Methanogenesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="MCR_beta_N" evidence="10">
        <location>
            <begin position="6"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="domain" description="MCR_beta" evidence="9">
        <location>
            <begin position="189"/>
            <end position="436"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000951"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00002461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00011155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR000263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AEF95998.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="444" mass="47543" checksum="5B8C8869121497C7" modified="2011-07-27" version="1">MVIYEDRIDLYDERGKLLEENVPLEAISPMINPTIEKIINDVKRSVAVNLSGIQNALKTGAVGGKACFCPGREIDLPIVENAEIIAEKVKKLVQIQDGDDTNVKLINKGNQMLVQLPSKRLKMAADYTVSTLVTGAAVIQAIIDTFDVNMFDAPVVKTAVLGRYPQTVDFHGANIAALLGPPVMLEGLGYGLRNIMANHIVAITRKKTLNAVALSSILEQTAMFETGDAVGAFERLHLLGLAFQGLNANNLVYEIVKENGKNGTVGTVVASVVERALEDKVIRPMKTMPSGFTVYEPVDWALWNAYAAAGLVAAVMVNIGASRAAQGVASTVLYYNDILEYETGLPGVDFGRAEGTGVGFSFFSHSIYGGGGPGTFHGNHVVTRHSKGFAIPCAAAAMCLDAGTQMFSVERTSALVGTVYSAIDYLREPLKHVAIGAREVKDKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBJ8</accession>
    <name>F6BBJ8_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="4">PP-loop domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metig_0451</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96007.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798616.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96007">
        <property type="protein sequence ID" value="AEF96007"/>
        <property type="gene ID" value="Metig_0451"/>
    </dbReference>
    <dbReference type="GeneID" id="10643288"/>
    <dbReference type="KEGG" id="mig:Metig_0451"/>
    <dbReference type="HOGENOM" id="CLU_830567_0_0_2"/>
    <dbReference type="OMA" id="DECPYSR"/>
    <dbReference type="OrthoDB" id="30824at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006400">
        <property type="term" value="P:tRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011063">
        <property type="entry name" value="TilS/TtcA_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035107">
        <property type="entry name" value="tRNA_thiolation_TtcA_Ctu1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01171">
        <property type="entry name" value="ATP_bind_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004976">
        <property type="entry name" value="ATPase_YdaO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="ATP_bind_3" evidence="3">
        <location>
            <begin position="78"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="290"/>
            <end position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEF96007.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="335" mass="38912" checksum="817F1881EDF4D555" modified="2011-07-27" version="1">MIDIKQLKRYANPSYLTIREDKILVCNKREARLSREKMNKIEEEFGIPVIYSRTYEEVSKKVGRFIIRNKIINPRDIIVVGLSGGKDSLMLLHLLEPYRRKYGIDIHAITVDLNVDGIRPWSEDREGMKLIKDHCKKLNIPHKVLKCDKNVVELSRILSENDNGIEYSPCFSCSIIRRHLLTKYADSLKEKEGRDVKIAFGHTLEDNSDTVLANILKGCAIKALKPIKQFYENVVDYKYFKITLKPCTIIRPLLPVSEKSIIKALNECGIEYYRDKDECPYSRNRGDNIRRRAHEVLSLLEKEVKNVREMVVSAAIKSENKMKKSEEKENEVIDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBL0</accession>
    <name>F6BBL0_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Dinitrogenase iron-molybdenum cofactor biosynthesis protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0463</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96019.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798628.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0463"/>
    <dbReference type="EnsemblBacteria" id="AEF96019">
        <property type="protein sequence ID" value="AEF96019"/>
        <property type="gene ID" value="Metig_0463"/>
    </dbReference>
    <dbReference type="GeneID" id="10643300"/>
    <dbReference type="KEGG" id="mig:Metig_0463"/>
    <dbReference type="HOGENOM" id="CLU_104194_2_3_2"/>
    <dbReference type="OMA" id="QFCIDQG"/>
    <dbReference type="OrthoDB" id="118295at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00851">
        <property type="entry name" value="MTH1175"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003731">
        <property type="entry name" value="Di-Nase_FeMo-co_biosynth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036105">
        <property type="entry name" value="DiNase_FeMo-co_biosyn_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033913">
        <property type="entry name" value="MTH1175_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02579">
        <property type="entry name" value="Nitro_FeMo-Co"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53146">
        <property type="entry name" value="SSF53146"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nitro_FeMo-Co" evidence="1">
        <location>
            <begin position="9"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02579"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96019.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="109" mass="12019" checksum="1D35D68BCFD0170A" modified="2011-07-27" version="1">MKIAIPTENGKISPHFGRCEEFVIVEIEDGKIKNKEIVENTARGGMHGVGTTAASIVGNYNVDILIVQNIGPKAYDVFKKLGIDVYKCNTSSIDECVKLFLEGKLEKFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBN4</accession>
    <name>F6BBN4_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="3">5-oxoprolinase (ATP-hydrolyzing)</fullName>
            <ecNumber evidence="3">3.5.2.9</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_0487</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.5.2.9" evidence="3"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96043.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798651.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0487"/>
    <dbReference type="EnsemblBacteria" id="AEF96043">
        <property type="protein sequence ID" value="AEF96043"/>
        <property type="gene ID" value="Metig_0487"/>
    </dbReference>
    <dbReference type="GeneID" id="10643324"/>
    <dbReference type="KEGG" id="mig:Metig_0487"/>
    <dbReference type="HOGENOM" id="CLU_002157_1_2_2"/>
    <dbReference type="OMA" id="HAMTVSV"/>
    <dbReference type="OrthoDB" id="4114at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017168">
        <property type="term" value="F:5-oxoprolinase (ATP-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008040">
        <property type="entry name" value="Hydant_A_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002821">
        <property type="entry name" value="Hydantoinase_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045079">
        <property type="entry name" value="Oxoprolinase_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11365">
        <property type="entry name" value="PTHR11365"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05378">
        <property type="entry name" value="Hydant_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01968">
        <property type="entry name" value="Hydantoinase_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="SSF53067"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Hydant_A_N" evidence="2">
        <location>
            <begin position="5"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="domain" description="Hydantoinase_A" evidence="1">
        <location>
            <begin position="203"/>
            <end position="500"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF96043.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="687" mass="76571" checksum="E9735C5F1175A36B" modified="2011-07-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBS1</accession>
    <name>F6BBS1_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Myo-inositol-1-phosphate synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_1669</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the myo-inositol 1-phosphate synthase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97201.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1669"/>
    <dbReference type="EnsemblBacteria" id="AEF97201">
        <property type="protein sequence ID" value="AEF97201"/>
        <property type="gene ID" value="Metig_1669"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_1669"/>
    <dbReference type="HOGENOM" id="CLU_050011_0_0_2"/>
    <dbReference type="OMA" id="YEVNAFY"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004512">
        <property type="term" value="F:inositol-3-phosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006021">
        <property type="term" value="P:inositol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002587">
        <property type="entry name" value="Myo-inos-1-P_Synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013021">
        <property type="entry name" value="Myo-inos-1-P_Synthase_GAPDH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01658">
        <property type="entry name" value="Inos-1-P_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07994">
        <property type="entry name" value="NAD_binding_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015578">
        <property type="entry name" value="Myoinos-ppht_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Inos-1-P_synth" evidence="2">
        <location>
            <begin position="215"/>
            <end position="320"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF97201.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="385" mass="42831" checksum="EDFF05BC2F753210" modified="2011-07-27" version="1">MGVVRALILGQGFVASIFAIGVERIKRGEIDCYGVPLANELPIKIEDIEIVGSYDVDKSKIGKTIYEVAKNYWDGEIPETLKNVTIRKGIHLRSLRNLPIEAEGLEDEMSLKEAVEKLVEEWKELKPDVIVNTCTTEAFVPFGNKEELIKAIEENNKDRLTATQVYAYAAALYAKEVGGAAFVNTIPTLIANDPAFVELAKECDLVIFGDDGATGATPLTADVLAHLAQRNRYVKDIAQFNIGGNNDFLALTDKERNKSKEYTKSSIVKDILGYDTPHYIKPTGYLEPLGDKKFISMHMEYISFNGAVDELIINGRINDSPALAGLLVDLVRLGKIAIDKKEFGTVYPVNAFYMKNPGPKDMPNIPRTIAYEKLRMWAGLKPRWL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-06-02" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBU9</accession>
    <name>F6BBU9_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_1697</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97229.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF97229">
        <property type="protein sequence ID" value="AEF97229"/>
        <property type="gene ID" value="Metig_1697"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_1697"/>
    <dbReference type="HOGENOM" id="CLU_181218_0_0_2"/>
    <dbReference type="OMA" id="YIEVYFG"/>
    <dbReference type="OrthoDB" id="123447at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019208">
        <property type="entry name" value="DUF2097"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09870">
        <property type="entry name" value="DUF2097"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF97229.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="99" mass="11341" checksum="EDAD8B26B03D5598" modified="2011-07-27" version="1">MKGHFAPKMCIEKEVDPEEILEYMHKNIEEGDYIEVYFGRVHVEGTVVVASDGFFRVDTDNDLLGTLEFDVSSIIDDLIELVHIKEDNAEEKIILRVKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBW8</accession>
    <name>F6BBW8_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Methanogenesis marker protein 8</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_1716</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97248.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799837.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1716"/>
    <dbReference type="EnsemblBacteria" id="AEF97248">
        <property type="protein sequence ID" value="AEF97248"/>
        <property type="gene ID" value="Metig_1716"/>
    </dbReference>
    <dbReference type="GeneID" id="10644590"/>
    <dbReference type="KEGG" id="mig:Metig_1716"/>
    <dbReference type="HOGENOM" id="CLU_982163_0_0_2"/>
    <dbReference type="OMA" id="QGLCGRI"/>
    <dbReference type="OrthoDB" id="45819at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009181">
        <property type="entry name" value="UCP004929_methan"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09872">
        <property type="entry name" value="DUF2099"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004929">
        <property type="entry name" value="UCP004929"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03275">
        <property type="entry name" value="methan_mark_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF97248.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="284" mass="31349" checksum="F8A611BA8EEAA936" modified="2011-07-27" version="1">MEDIHIMEALGKAKVVVKNGKVVDVGEPKIKYCPLFDKHRGIKEIDKESIRKNIEFRIKDFGLFTENRIVEVEDCIVGFGTSEIFMTALRRGLLDAVVIVADCAGTVITNNPKLVQGLCGRISGIVKTSPIPKVIERIEKAGGYVLDPKTAEINQIKGVEKAIELGYKRIGVSLTNLEDAIKCKSLENEEIKIITFGVHTTGIDENPEELTKYFDLITACASKMVREGLKGKIKAQIGVSVPIFALSQIGKELLLERAKDVEKPILINVEDLPCLKGRQPDPLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>F6BC03</accession>
    <name>F6BC03_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ribonuclease Z</fullName>
            <shortName evidence="8">RNase Z</shortName>
            <ecNumber evidence="8">3.1.26.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">tRNA 3 endonuclease</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">tRNase Z</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">rnz</name>
        <name evidence="10" type="ordered locus">Metig_0529</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Zinc phosphodiesterase, which displays some tRNA 3'-processing endonuclease activity. Probably involved in tRNA maturation, by removing a 3'-trailer from precursor tRNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>Endonucleolytic cleavage of RNA, removing extra 3' nucleotides from tRNA precursor, generating 3' termini of tRNAs. A 3'-hydroxy group is left at the tRNA terminus and a 5'-phosphoryl group is left at the trailer molecule.</text>
            <dbReference type="EC" id="3.1.26.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="8">Binds 2 Zn(2+) ions.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1 8">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the RNase Z family.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.11" evidence="8"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96084.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798692.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0529"/>
    <dbReference type="EnsemblBacteria" id="AEF96084">
        <property type="protein sequence ID" value="AEF96084"/>
        <property type="gene ID" value="Metig_0529"/>
    </dbReference>
    <dbReference type="GeneID" id="10643367"/>
    <dbReference type="KEGG" id="mig:Metig_0529"/>
    <dbReference type="HOGENOM" id="CLU_031317_2_1_2"/>
    <dbReference type="OMA" id="GTQRQMM"/>
    <dbReference type="OrthoDB" id="111803at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042781">
        <property type="term" value="F:3'-tRNA processing endoribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07717">
        <property type="entry name" value="RNaseZ_ZiPD-like_MBL-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01818">
        <property type="entry name" value="RNase_Z_BN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013471">
        <property type="entry name" value="RNase_Z/BN"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12706">
        <property type="entry name" value="Lactamase_B_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="SSF56281"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02651">
        <property type="entry name" value="RNase_Z"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="6 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 8" id="KW-0540">Nuclease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="7 8" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Lactamase_B" evidence="9">
        <location>
            <begin position="18"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="8">
        <location>
            <position position="64"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="60"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="62"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="64"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="65"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="140"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="210"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="270"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01818"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AEF96084.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="317" mass="35413" checksum="1009DDF9E634A5AC" modified="2011-07-27" version="1">MKLIFLGTGAAVPTKKRAHASIALKINGEVLLFDCGENAQRQMMYTEVSPMKIKNIFISHLHGDHILGIPGLLQSMAFAGRKEEINIYGPVGTKEMMIHALSVGYHGINFKVNVYEINSKEAVKIIDNEKFEVYAFPVKHSVPSYAYVFKEKKKPRLDINKAKALGVKVGPDLKKLKDGIPVKLEDGRVIKPEEVLLPPKKGICVGYSGDTVPIEEFGKFLKELGCGVLIHEATYDNTLKENAIETMHSTVEDAIKIAEIAKVETLILTHISARYDDDISIYEKQIKEYNGDMDIIIAEDFMVYDLKKKTCSISILD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>F6BC10</accession>
    <name>F6BC10_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ornithine carbamoyltransferase, catabolic</fullName>
            <shortName evidence="4">OTCase</shortName>
            <ecNumber evidence="4">2.1.3.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">arcB</name>
        <name evidence="8" type="ordered locus">Metig_0536</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 4">
            <text>carbamoyl phosphate + L-ornithine = H(+) + L-citrulline + phosphate</text>
            <dbReference type="Rhea" id="RHEA:19513"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46911"/>
            <dbReference type="ChEBI" id="CHEBI:57743"/>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
            <dbReference type="EC" id="2.1.3.3"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">Amino-acid degradation; L-arginine degradation via ADI pathway; carbamoyl phosphate from L-arginine: step 2/2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the aspartate/ornithine carbamoyltransferase superfamily. OTCase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.1.3.3" evidence="4"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96091.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798698.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0536"/>
    <dbReference type="EnsemblBacteria" id="AEF96091">
        <property type="protein sequence ID" value="AEF96091"/>
        <property type="gene ID" value="Metig_0536"/>
    </dbReference>
    <dbReference type="GeneID" id="10643374"/>
    <dbReference type="KEGG" id="mig:Metig_0536"/>
    <dbReference type="HOGENOM" id="CLU_043846_3_2_2"/>
    <dbReference type="OMA" id="DGNNVCN"/>
    <dbReference type="OrthoDB" id="43727at2157"/>
    <dbReference type="UniPathway" id="UPA00254">
        <property type="reaction ID" value="UER00365"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016597">
        <property type="term" value="F:amino acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004585">
        <property type="term" value="F:ornithine carbamoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019547">
        <property type="term" value="P:arginine catabolic process to ornithine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019546">
        <property type="term" value="P:arginine deiminase pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1370">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01109">
        <property type="entry name" value="OTCase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006132">
        <property type="entry name" value="Asp/Orn_carbamoyltranf_P-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006130">
        <property type="entry name" value="Asp/Orn_carbamoylTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036901">
        <property type="entry name" value="Asp/Orn_carbamoylTrfase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006131">
        <property type="entry name" value="Asp_carbamoyltransf_Asp/Orn-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002292">
        <property type="entry name" value="Orn/put_carbamltrans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024904">
        <property type="entry name" value="OTCase_ArgI"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00185">
        <property type="entry name" value="OTCace"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02729">
        <property type="entry name" value="OTCace_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00100">
        <property type="entry name" value="AOTCASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00102">
        <property type="entry name" value="OTCASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53671">
        <property type="entry name" value="SSF53671"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00658">
        <property type="entry name" value="orni_carb_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00097">
        <property type="entry name" value="CARBAMOYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0056">Arginine metabolism</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="OTCace_N" evidence="7">
        <location>
            <begin position="2"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="domain" description="OTCace" evidence="6">
        <location>
            <begin position="145"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="region of interest" description="Carbamoyl phosphate binding" evidence="4">
        <location>
            <begin position="48"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Carbamoyl phosphate binding" evidence="4">
        <location>
            <begin position="126"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Ornithine binding" evidence="4">
        <location>
            <begin position="226"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="region of interest" description="Carbamoyl phosphate binding" evidence="4">
        <location>
            <begin position="262"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="99"/>
        </location>
        <ligand>
            <name>carbamoyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="157"/>
        </location>
        <ligand>
            <name>L-ornithine</name>
            <dbReference type="ChEBI" id="CHEBI:46911"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="222"/>
        </location>
        <ligand>
            <name>L-ornithine</name>
            <dbReference type="ChEBI" id="CHEBI:46911"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="290"/>
        </location>
        <ligand>
            <name>carbamoyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEF96091.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="310" mass="35243" checksum="803E8E9E3061E53B" modified="2011-07-27" version="1">MHLLDLDVLRREDVEKILEYAEYFKKNRYNHEKILKDKSIAIIFEKPSTRTRMSFDIAVHELGGHSLIMNQNEIHLGKKESIKDTARVMSRYVDAIVARVYEHKTLEDLAKYGTVPVINALSNLAHPCQILADLLTIKEYKKSFKGKKIAYLGDGNNVCNSLILGSALVGMDMVVATPRGYEPNALNVLKALEIIDEYGEGSLKLTNDPIEAAEDADILYTDVWISMGDEKNLEHVMKIFPPFQINEKLLEYAKDDVIVMHCLPANRGMEITDEVIDGEHSVVYDEAENRLHAQKAIFKFIFEKSSSDIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>F6BC17</accession>
    <name>F6BC17_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Iron-chelate-transporting ATPase</fullName>
            <ecNumber evidence="5">3.6.3.34</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Metig_0544</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.6.3.34" evidence="5"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96098.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798705.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0544"/>
    <dbReference type="EnsemblBacteria" id="AEF96098">
        <property type="protein sequence ID" value="AEF96098"/>
        <property type="gene ID" value="Metig_0544"/>
    </dbReference>
    <dbReference type="GeneID" id="10643382"/>
    <dbReference type="KEGG" id="mig:Metig_0544"/>
    <dbReference type="HOGENOM" id="CLU_000604_0_0_2"/>
    <dbReference type="OMA" id="KNFFEMS"/>
    <dbReference type="OrthoDB" id="68573at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="2"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="34"/>
            <end position="41"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEF96098.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="380" mass="42654" checksum="399925AC5A27F290" modified="2011-07-27" version="1">MLKTENLAVGYHKRVVVNNVNLEVKEGEILAIIGPNGVGKSTLLKCIATYLEPIKGVVYLNSNIIHRLKPSQLAKKMAVVLTERVNPGNLTGFDIIAIGRHPYTDLFGRLSEKDKEIIINSAKAVNALHLLEKNFLEMSDGERQKIMIARALAQEPKVLILDEPTSFLDARHKIELTLLLRKLAIENNLAIVVTIHDIELALRIADKIALIKDGKIIAYGHPEDVMDKETVNNLYNIKNANFCKEIGYFELKNENTADKKVFVVCGGGSGANVLRYLVKSGYKIYCGVLHENDIDYIVAKTMEVEIIKEKAYQPISEENYIKALNYLKMCDVVIDTNFPIGEMNKLNLKLIEEAKKSNKKVIKFNGSIKDLKNQLLSKLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>F6BC37</accession>
    <name>F6BC37_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">2-phosphosulfolactate phosphatase</fullName>
            <ecNumber evidence="3">3.1.3.71</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Metig_0564</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>(2R)-O-phospho-3-sulfolactate + H2O = (2R)-3-sulfolactate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:23416"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15597"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58738"/>
            <dbReference type="EC" id="3.1.3.71"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ComB family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.71" evidence="3"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96118.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0564"/>
    <dbReference type="EnsemblBacteria" id="AEF96118">
        <property type="protein sequence ID" value="AEF96118"/>
        <property type="gene ID" value="Metig_0564"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0564"/>
    <dbReference type="HOGENOM" id="CLU_070028_0_1_2"/>
    <dbReference type="OMA" id="RLFMSTT"/>
    <dbReference type="OrthoDB" id="84054at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050532">
        <property type="term" value="F:2-phosphosulfolactate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019295">
        <property type="term" value="P:coenzyme M biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1560.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005238">
        <property type="entry name" value="ComB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036702">
        <property type="entry name" value="ComB-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027639">
        <property type="entry name" value="ComB_archaeal"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37311">
        <property type="entry name" value="PTHR37311"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04029">
        <property type="entry name" value="2-ph_phosp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF142823">
        <property type="entry name" value="SSF142823"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00298">
        <property type="entry name" value="TIGR00298"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00033711"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEF96118.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="241" mass="26755" checksum="E0F979C32DDAB77D" modified="2011-07-27" version="1">MPQTAINLTYEFFSVGCENSKSDVAIVIDVLRASTTINTLLQLCNEVYITDSIEKANSYKDAIKIGERNGKKIEKFDFGNSPTEILRNKGKILEHVKNNGKVVLTTTNGTRVMKRVFKNANYVLIGSITNAKYVAEKAVELAKSGITLVPCHRKGTFAIEDVIGAGLIARYIEEISKEKTKNEEVLAAKLLTKGDWKSLILNSNSADNLRNLGYHEDLLFCICENAQDVVGMFDGEKVIRM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>F6BC45</accession>
    <name>F6BC45_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Fe(3+)-transporting ATPase</fullName>
            <ecNumber evidence="5">3.6.3.30</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Metig_0572</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.6.3.30" evidence="5"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96126.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0572"/>
    <dbReference type="EnsemblBacteria" id="AEF96126">
        <property type="protein sequence ID" value="AEF96126"/>
        <property type="gene ID" value="Metig_0572"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0572"/>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_2"/>
    <dbReference type="OMA" id="VRQADRY"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="2"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="34"/>
            <end position="41"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEF96126.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="237" mass="26309" checksum="10995966D5EAD119" modified="2011-07-27" version="1">MIKVENLSAGYGKLQILFEVNASAEKGKITTIVGPNGSGKSTFLKTLFGLTTIYSGKIYFKDEDITNLPPHKKTQRGLAYLPQTNNVFANLTVEENLKIAGYLLPKDEIKDRIDLALSVFPELKNILKRKAGQLSGGQRQFLAMATALVRKSEVLMLDEPTAQLSPKLAETIFNKILELRDDYGLTILLVEQNAKRALEISDRGYMLVSGRVAYEGSAKGLLESEKFKSYFLGLIEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>F6BC86</accession>
    <name>F6BC86_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">2,3-bisphosphoglycerate-independent phosphoglycerate mutase</fullName>
            <shortName evidence="7">BPG-independent PGAM</shortName>
            <shortName evidence="7">Phosphoglyceromutase</shortName>
            <shortName evidence="7">aPGAM</shortName>
            <ecNumber evidence="7">5.4.2.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">apgM</name>
        <name evidence="9" type="ordered locus">Metig_1760</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 7">Catalyzes the interconversion of 2-phosphoglycerate and 3-phosphoglycerate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>(2R)-2-phosphoglycerate = (2R)-3-phosphoglycerate</text>
            <dbReference type="Rhea" id="RHEA:15901"/>
            <dbReference type="ChEBI" id="CHEBI:58272"/>
            <dbReference type="ChEBI" id="CHEBI:58289"/>
            <dbReference type="EC" id="5.4.2.12"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 7">Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 3/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 7">Belongs to the BPG-independent phosphoglycerate mutase family. A-PGAM subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.4.2.12" evidence="7"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97292.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799881.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1760"/>
    <dbReference type="EnsemblBacteria" id="AEF97292">
        <property type="protein sequence ID" value="AEF97292"/>
        <property type="gene ID" value="Metig_1760"/>
    </dbReference>
    <dbReference type="GeneID" id="10644634"/>
    <dbReference type="KEGG" id="mig:Metig_1760"/>
    <dbReference type="HOGENOM" id="CLU_034906_2_0_2"/>
    <dbReference type="OMA" id="IAFRCNF"/>
    <dbReference type="OrthoDB" id="17268at2157"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00186"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046537">
        <property type="term" value="F:2,3-bisphosphoglycerate-independent phosphoglycerate mutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd16011">
        <property type="entry name" value="iPGM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01402_A">
        <property type="entry name" value="ApgM_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023665">
        <property type="entry name" value="ApgAM_prokaryotes"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006124">
        <property type="entry name" value="Metalloenzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004456">
        <property type="entry name" value="Pglycerate_mutase_ApgM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042253">
        <property type="entry name" value="Pglycerate_mutase_ApgM_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31209">
        <property type="entry name" value="PTHR31209"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01676">
        <property type="entry name" value="Metalloenzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10143">
        <property type="entry name" value="PhosphMutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006392">
        <property type="entry name" value="IPGAM_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="SSF53649"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00306">
        <property type="entry name" value="apgM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="6 7 9" id="KW-0413">Isomerase</keyword>
    <keyword evidence="9" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metalloenzyme" evidence="8">
        <location>
            <begin position="1"/>
            <end position="395"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AEF97292.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="406" mass="44599" checksum="F457AEEAF7D23E15" modified="2011-07-27" version="1">MKAVIFVIDGLGDRPNKEGNTPLKEANTPTMDKIAKEGICGIMNAIDIGVRPGSDTSHLAILGYDPYEVYTGRGPFEACGVGIEVKPGDVAFRCNFATVDENFVVLDRRAGRIRETEALAKELDGLEIDGVKVIFKQSGGYRAALVLRGEGLSDKVTDADPKKEGRKVKTVEPLDDSEEAKRTAEILNKLLKIAYEKLNNHPINEERRKKGLPVANIILPRGAGKVPYIQKFEEKYGLKGACIAGTGLIKGIGKMVGLDLIEVEGANGTPETNFMGKARALVEALEKYDFVLINVKGADEAGHDGDYETKKKVIEKIDEMLKYIIEHIDKDDVYLVLTGDHSTPIEEKDHSADPIPIVIWGRSVRTDDVERFDEFSTYKGGLGWIKGKHIMPILMDLMGLAKKYGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCB1</accession>
    <name>F6BCB1_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">NIL domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1785</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97317.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1785"/>
    <dbReference type="EnsemblBacteria" id="AEF97317">
        <property type="protein sequence ID" value="AEF97317"/>
        <property type="gene ID" value="Metig_1785"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_1785"/>
    <dbReference type="HOGENOM" id="CLU_152999_0_0_2"/>
    <dbReference type="OMA" id="TGVCPTE"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018449">
        <property type="entry name" value="NIL_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09383">
        <property type="entry name" value="NIL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00930">
        <property type="entry name" value="NIL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="SSF55021"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="75"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="105"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF97317.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="134" mass="15231" checksum="165C7CE192045519" modified="2011-07-27" version="1">MLMKKRIYLWVPSENVHKPIISNAILNTKININILKAKIEPQEAFLILELNGDEDKVNEAIEYLSKYGEIEEISKVIKRDEEKCVDCGACITMCPVEAIKMDDDFSVVFDENECIGCKSCVKVCPFKAIEVDDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCC5</accession>
    <name>F6BCC5_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 10">tRNA (cytidine(56)-2'-O)-methyltransferase</fullName>
            <ecNumber evidence="6 10">2.1.1.206</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9 10">tRNA ribose 2'-O-methyltransferase aTrm56</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">Metig_1799</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 10">Specifically catalyzes the AdoMet-dependent 2'-O-ribose methylation of cytidine at position 56 in tRNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>cytidine(56) in tRNA + S-adenosyl-L-methionine = 2'-O-methylcytidine(56) in tRNA + H(+) + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:42968"/>
            <dbReference type="Rhea" id="RHEA-COMP:10308"/>
            <dbReference type="Rhea" id="RHEA-COMP:10309"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74495"/>
            <dbReference type="ChEBI" id="CHEBI:82748"/>
            <dbReference type="EC" id="2.1.1.206"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="5 10">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3 10">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 10">Belongs to the aTrm56 family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.206" evidence="6 10"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97331.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799920.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1799"/>
    <dbReference type="EnsemblBacteria" id="AEF97331">
        <property type="protein sequence ID" value="AEF97331"/>
        <property type="gene ID" value="Metig_1799"/>
    </dbReference>
    <dbReference type="GeneID" id="10644676"/>
    <dbReference type="KEGG" id="mig:Metig_1799"/>
    <dbReference type="HOGENOM" id="CLU_123709_0_0_2"/>
    <dbReference type="OMA" id="VVHLTMY"/>
    <dbReference type="OrthoDB" id="83050at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008175">
        <property type="term" value="F:tRNA methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002128">
        <property type="term" value="P:tRNA nucleoside ribose methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1280.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00077">
        <property type="entry name" value="tRNA_methyltr_aTrm56"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029028">
        <property type="entry name" value="Alpha/beta_knot_MTases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029026">
        <property type="entry name" value="tRNA_m1G_MTases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002845">
        <property type="entry name" value="tRNA_mtfrase_aTrm56"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42197">
        <property type="entry name" value="PTHR42197"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01994">
        <property type="entry name" value="Trm56"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016123">
        <property type="entry name" value="UCP016123"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75217">
        <property type="entry name" value="SSF75217"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10 11" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="10 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 10" id="KW-0819">tRNA processing</keyword>
    <feature type="region of interest" description="S-adenosyl-L-methionine binding" evidence="10">
        <location>
            <begin position="111"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="S-adenosyl-L-methionine binding" evidence="10">
        <location>
            <begin position="129"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="83"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00013709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00029826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AEF97331.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="179" mass="20625" checksum="04404A0F032E8E58" modified="2011-07-27" version="1">MRVEVLRLGHRGERDKRISTHVALTARALGADKIIFTVEDEHVEESVKKIVENWGGNFKFEVIKKWKEYIKNFKENNGIVVHLTMYGANVNEIMDEIKKEFEGKNILVIVGAEKVPKEAYELADYNVSIGNQPHSEVAALAIFLDRLFEGKTLYRKFENAKIRIIPSNDKKQVIIKKDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCC6</accession>
    <name>F6BCC6_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Radical SAM domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Metig_1800</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="6">Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97332.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799921.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1800"/>
    <dbReference type="EnsemblBacteria" id="AEF97332">
        <property type="protein sequence ID" value="AEF97332"/>
        <property type="gene ID" value="Metig_1800"/>
    </dbReference>
    <dbReference type="GeneID" id="10644677"/>
    <dbReference type="KEGG" id="mig:Metig_1800"/>
    <dbReference type="HOGENOM" id="CLU_044176_1_0_2"/>
    <dbReference type="OMA" id="YHFYPGS"/>
    <dbReference type="OrthoDB" id="39920at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027596">
        <property type="entry name" value="AmmeMemoSam_rS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaB/NifB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034457">
        <property type="entry name" value="Organic_radical-activating"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016431">
        <property type="entry name" value="Pyrv-formate_lyase-activ_prd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30352">
        <property type="entry name" value="PTHR30352"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004869">
        <property type="entry name" value="PflX_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01101">
        <property type="entry name" value="Uncharacterised_Radical_SAM_Su"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR04337">
        <property type="entry name" value="AmmeMemoSam_rS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="4 6" id="KW-0408">Iron</keyword>
    <keyword evidence="5 6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="7">
        <location>
            <begin position="68"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="83"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="87"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="90"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR004869-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEF97332.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="337" mass="38484" checksum="40F40413280BCFE1" modified="2011-07-27" version="1">MLKEAMFYEELEDNKVRCNLCPRHCIILEGKRGFCRARKNIGGKLYAMGYGKLSSVAIDPIEKKPLFHFYPGSSVLSIATGGCNFRCKHCQNWQISQKAPDEIPYREMYPEDIVNLAVEYNCEGISYTYTEPTIFYEIMYDTAILARKHGLYNAMVTNGYIEEEPLKKLPVDAMNIDIKGNAEFYKKVCQGTLDPVLRTCVLAKKLGIHVEVTNLIIPGYNDNEDDILYIIEFVKERLGEETPLHFTRFHPDYMLMDVPPTPVEILEKARNMALEEGLKYVYIGNVPGHEGEHTYCPNCGALLIRRDIYTISIVNLDLLTKPPRCSLCGEKIDIITK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCC7</accession>
    <name>F6BCC7_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_1801</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97333.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1801"/>
    <dbReference type="EnsemblBacteria" id="AEF97333">
        <property type="protein sequence ID" value="AEF97333"/>
        <property type="gene ID" value="Metig_1801"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_1801"/>
    <dbReference type="HOGENOM" id="CLU_107938_2_0_2"/>
    <dbReference type="OMA" id="RTYNIIH"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1410.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028978">
        <property type="entry name" value="Chorismate_lyase_/UTRA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002800">
        <property type="entry name" value="Rv2949c-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01947">
        <property type="entry name" value="DUF98"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64288">
        <property type="entry name" value="SSF64288"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF97333.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="171" mass="20064" checksum="D7C745F03A0927A3" modified="2011-07-27" version="1">MYFGESMKIYERIRELSRKYGLRNEEKMILGTDGSVTNLLEILFEGDVIVKTINQKIVDDVNYRTVILEVNDIPLVYATSKTPFKNIEHGLRESIKRDLLSADIPIGRILKMHNLETRREIIDIDVKEVDKEVRKLLKTDRKILPQRTYNIIHNNKILMQITEIFNVGEHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCE5</accession>
    <name>F6BCE5_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Appr-1-p processing domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0602</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96156.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798762.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0602"/>
    <dbReference type="EnsemblBacteria" id="AEF96156">
        <property type="protein sequence ID" value="AEF96156"/>
        <property type="gene ID" value="Metig_0602"/>
    </dbReference>
    <dbReference type="GeneID" id="10643440"/>
    <dbReference type="KEGG" id="mig:Metig_0602"/>
    <dbReference type="HOGENOM" id="CLU_046550_7_0_2"/>
    <dbReference type="OMA" id="HYGKGLP"/>
    <dbReference type="OrthoDB" id="78043at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.220.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002589">
        <property type="entry name" value="Macro_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043472">
        <property type="entry name" value="Macro_dom-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01661">
        <property type="entry name" value="Macro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00506">
        <property type="entry name" value="A1pp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52949">
        <property type="entry name" value="SSF52949"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51154">
        <property type="entry name" value="MACRO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Macro" evidence="1">
        <location>
            <begin position="1"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96156.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="184" mass="20171" checksum="69BDC223A1EB54DF" modified="2011-07-27" version="1">MENSLKINKTTIVVKKGDITKENVDAIVNAANPYLQHGGGVAWAIVKDNPIIQKESDRLIMEIGRVEVGNAVYTTGGNLAKYVIHAVGPKWGEGDEERKLRNAIYNALKLATELKVKSIAIPAVSCGTYGFPKEKGTEIIINTIVDFLKNNKTTLELIKCVDIDDEVVEGFNKALNKVKMNFNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCJ9</accession>
    <name>F6BCJ9_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">GCN5-related N-acetyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0660</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96210.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0660"/>
    <dbReference type="EnsemblBacteria" id="AEF96210">
        <property type="protein sequence ID" value="AEF96210"/>
        <property type="gene ID" value="Metig_0660"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0660"/>
    <dbReference type="HOGENOM" id="CLU_013985_34_9_2"/>
    <dbReference type="OMA" id="VIFVARY"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="2"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96210.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="150" mass="17032" checksum="777F99EBD914B89A" modified="2011-07-27" version="1">MITIDTAKKEDIDDMINLLKQLFEIEKDFTPNYEKQRKGLELLLNSKNAVIFVARYNGKVVGMCSIQTLISTAEGGKVGILEDLVVDENFRGKGIGSKLLSEAERYCKEKGGLLRLSLLADKDNKKALEFYKSRGWKFTNLICLRKFFGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCM3</accession>
    <name>F6BCM3_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transketolase</fullName>
            <ecNumber evidence="2">2.2.1.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0684</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.2.1.1" evidence="2"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96234.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798837.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0684"/>
    <dbReference type="EnsemblBacteria" id="AEF96234">
        <property type="protein sequence ID" value="AEF96234"/>
        <property type="gene ID" value="Metig_0684"/>
    </dbReference>
    <dbReference type="GeneID" id="10643524"/>
    <dbReference type="KEGG" id="mig:Metig_0684"/>
    <dbReference type="HOGENOM" id="CLU_009227_1_1_2"/>
    <dbReference type="OMA" id="VINTCDY"/>
    <dbReference type="OrthoDB" id="56448at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004802">
        <property type="term" value="F:transketolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006082">
        <property type="term" value="P:organic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901576">
        <property type="term" value="P:organic substance biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044272">
        <property type="term" value="P:sulfur compound biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009014">
        <property type="entry name" value="Transketo_C/PFOR_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005475">
        <property type="entry name" value="Transketolase-like_Pyr-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033248">
        <property type="entry name" value="Transketolase_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02779">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02780">
        <property type="entry name" value="Transketolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00861">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="SSF52518"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52922">
        <property type="entry name" value="SSF52922"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Transket_pyr" evidence="1">
        <location>
            <begin position="9"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96234.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="316" mass="34597" checksum="E1CCD3FEC9C26E44" modified="2011-07-27" version="1">MVKLSGIKKGMRNGYGETLIELGEKYENLVVLDADLSGSTKTAMFAKKFPERFFNAGIAEQNMIGMAAGLATTGKIVFASTFAMFATGRAWEQIRNSVAYPRLNVKIVATHSGITVGEDGATHQMTEDIAIMRAIPNMVVIAPSDYYHTKNIIRWCAEYDGPVYVRMPRSDTEIIYESEEEAEFEVGKAKTLIDGDDLTIIATGEEVPEALRAAEILKENGISAEVVEMATIKPIDEDAIKKAKDFIVSMEDHSIIGGLGGAIAEVIATNGLNKRLLRIGINDEFGKSGKAEELLKYYGLDGESVAKRIMKELKKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCM6</accession>
    <name>F6BCM6_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Amidohydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0687</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96237.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798840.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0687"/>
    <dbReference type="EnsemblBacteria" id="AEF96237">
        <property type="protein sequence ID" value="AEF96237"/>
        <property type="gene ID" value="Metig_0687"/>
    </dbReference>
    <dbReference type="GeneID" id="10643527"/>
    <dbReference type="KEGG" id="mig:Metig_0687"/>
    <dbReference type="HOGENOM" id="CLU_012358_1_0_2"/>
    <dbReference type="OMA" id="TDNFMAN"/>
    <dbReference type="OrthoDB" id="75040at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006680">
        <property type="entry name" value="Amidohydro-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01979">
        <property type="entry name" value="Amidohydro_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="SSF51556"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidohydro-rel" evidence="1">
        <location>
            <begin position="43"/>
            <end position="348"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96237.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="379" mass="42477" checksum="032C54BBF770BB74" modified="2011-07-27" version="1">MYLQSNFLYGENFELKKGTLVIEGGVIKGFTNEKVNAIKYKGIVIPSLINAHTHVGDAAIKDIGINKTLDELVKPPNGLKHKFLNSCSDEELINGMLCALNELFENGIRYFCDFRENGIKGINLLKKAISLSELNIDAIILGRPTKIIKEEIKKELNEILDNCSGIGLSGANEYSDEALKFIYRCTKDKDRIFAIHASEHEGSLKYSLEHYGKTEIERIIDLKVKPNFIIHATHATPNDMELLKENGIGVVVCPRANASFNVGLPNIRALLEHGILVGIGTDNFMANSPSIFKDMDFIYKIYHIEPKEILKMATINNAKILGIENVGIIKEGYKPSFTFIKPTNAIKYSKNIVASIITRCERGDIDLYFMKEFAQLFTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCN0</accession>
    <name>F6BCN0_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Thioredoxin reductase</fullName>
            <ecNumber evidence="2">1.8.1.9</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0691</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.8.1.9" evidence="2"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96241.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798844.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0691"/>
    <dbReference type="EnsemblBacteria" id="AEF96241">
        <property type="protein sequence ID" value="AEF96241"/>
        <property type="gene ID" value="Metig_0691"/>
    </dbReference>
    <dbReference type="GeneID" id="10643531"/>
    <dbReference type="KEGG" id="mig:Metig_0691"/>
    <dbReference type="HOGENOM" id="CLU_031864_5_3_2"/>
    <dbReference type="OMA" id="VDNFPGY"/>
    <dbReference type="OrthoDB" id="41707at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004791">
        <property type="term" value="F:thioredoxin-disulfide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019430">
        <property type="term" value="P:removal of superoxide radicals"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005982">
        <property type="entry name" value="Thioredox_Rdtase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01292">
        <property type="entry name" value="TRX_reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pyr_redox_2" evidence="1">
        <location>
            <begin position="3"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96241.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="301" mass="32921" checksum="2A01BDA06C3073CA" modified="2011-07-27" version="1">MTYDTIIIGGGPAGLTAGIYAMRAKLNVLCVEKENAGGRIAEAGIVENYPGFDEIRGFELAQKFVEHAKKFGLPIVHEEVIRIDTSERPFKVITKNNEYKTKTIIIASGTRPKRLGLNEDKFVGRGVSYCTTCDAFFYLKKDVIVVGRDTPAVMSAINLKDIANKIYLITDKANIKVAEPIMLDRLKEAKNVEIIYNAKPIEIVGENKVEGLKIERNGKEEIVKADGIFVSLGHIPNTEFLEGSGIELRNGFVVVDKNCKTNIDGIYACGDVTGGVMQVSKAVGEGAIAMVNATKYIQRMG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-06-02" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCN1</accession>
    <name>F6BCN1_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_0692</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96242.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96242">
        <property type="protein sequence ID" value="AEF96242"/>
        <property type="gene ID" value="Metig_0692"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0692"/>
    <dbReference type="HOGENOM" id="CLU_830572_0_0_2"/>
    <dbReference type="OMA" id="IETYEYA"/>
    <dbReference type="OrthoDB" id="37109at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF96242.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="341" mass="40206" checksum="737F547E66E55C1C" modified="2011-07-27" version="1">MTACIVCRGCPNTIQNFANTYWRHIYPQYWNRVIEKYNLKPIISYEFTELPPHRFVGMMGNFISENITTGPCLLTPKEIKSLNKNTTTEELERMLMRHPTFEDYVSVAIENKYKTHIIIETYEYAKLIEYKTNIPFDDALKLTKKSAKNFKKYYDKKLYGRKVKYYLTHENKFDSRLRELCNEYYKFYLSNNNISKKAREIIEKNPEESTWLRIKVSFLPEAIDKESSTIIEPVSSAEGMVNAVKLSNASAIITRSPPTLTMKPVMNEGDENEIFYLNNNIEDEFKKLRYNTKSPFGCSVYNMMAFLQFDNHLLDLNCKSKRCEDCLELLKSHLEKIYIKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCS6</accession>
    <name>F6BCS6_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 9">Quinolinate synthase</fullName>
            <ecNumber evidence="2 9">2.5.1.72</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">nadA</name>
        <name evidence="10" type="ordered locus">Metig_0739</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the condensation of iminoaspartate with dihydroxyacetone phosphate to form quinolinate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>dihydroxyacetone phosphate + iminosuccinate = H(+) + 2 H2O + phosphate + quinolinate</text>
            <dbReference type="Rhea" id="RHEA:25888"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29959"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
            <dbReference type="ChEBI" id="CHEBI:77875"/>
            <dbReference type="EC" id="2.5.1.72"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="9">Binds 1 [4Fe-4S] cluster per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 9">Cofactor biosynthesis; NAD(+) biosynthesis; quinolinate from iminoaspartate: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the quinolinate synthase family. Type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.72" evidence="2 9"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96287.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798890.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0739"/>
    <dbReference type="EnsemblBacteria" id="AEF96287">
        <property type="protein sequence ID" value="AEF96287"/>
        <property type="gene ID" value="Metig_0739"/>
    </dbReference>
    <dbReference type="GeneID" id="10643578"/>
    <dbReference type="KEGG" id="mig:Metig_0739"/>
    <dbReference type="HOGENOM" id="CLU_047382_0_0_2"/>
    <dbReference type="OMA" id="FFLPDKC"/>
    <dbReference type="OrthoDB" id="18897at2157"/>
    <dbReference type="UniPathway" id="UPA00253">
        <property type="reaction ID" value="UER00327"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008987">
        <property type="term" value="F:quinolinate synthetase A activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009435">
        <property type="term" value="P:NAD biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10800">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00568">
        <property type="entry name" value="NadA_type2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003473">
        <property type="entry name" value="NadA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036094">
        <property type="entry name" value="NadA_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023066">
        <property type="entry name" value="Quinolinate_synth_type2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30573">
        <property type="entry name" value="PTHR30573"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02445">
        <property type="entry name" value="NadA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF142754">
        <property type="entry name" value="SSF142754"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00550">
        <property type="entry name" value="nadA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 9" id="KW-0408">Iron</keyword>
    <keyword evidence="8 9" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="6 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 9" id="KW-0662">Pyridine nucleotide biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9 10" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Iminoaspartate binding" evidence="9">
        <location>
            <begin position="110"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Iminoaspartate binding" evidence="9">
        <location>
            <begin position="197"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="21"/>
        </location>
        <ligand>
            <name>iminosuccinate</name>
            <dbReference type="ChEBI" id="CHEBI:77875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="38"/>
        </location>
        <ligand>
            <name>iminosuccinate</name>
            <dbReference type="ChEBI" id="CHEBI:77875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="84"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="127"/>
        </location>
        <ligand>
            <name>iminosuccinate</name>
            <dbReference type="ChEBI" id="CHEBI:77875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="171"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="214"/>
        </location>
        <ligand>
            <name>iminosuccinate</name>
            <dbReference type="ChEBI" id="CHEBI:77875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="263"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AEF96287.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="306" mass="34948" checksum="5B34B0D8A815E05F" modified="2011-07-27" version="1">MDIVQRISQLKEEKNAVILAHNYQREEIQRIADFVGDSLELCIKAKETDADIIVFCGVDFMAETAKILNSDKKVLIPEIKDIECPMAHQLPAEVVRKAKEEHPDAMVVVYVNTLAETKALADATCTSANADRVVNSLDCDKVLFGPDRNLAYFVSKRTDKEIIPIPEDGHCYVHKKFTVEDVKRVRREYPNAELLVHPECDPEVQDLADYVLSTGGMIRHVLNSDKKEFIIGTECELITRIKLELEKTGEEKVLIPLRKDAICDPMKRITLEKVERCLREEKYEITLDEKIIKKARIAIERMLSVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCT5</accession>
    <name>F6BCT5_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Restriction modification system DNA specificity domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Metig_0748</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the type-I restriction system S methylase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96296.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798899.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0748"/>
    <dbReference type="REBASE" id="36257">
        <property type="entry name" value="S.Mig5ORF746P"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96296">
        <property type="protein sequence ID" value="AEF96296"/>
        <property type="gene ID" value="Metig_0748"/>
    </dbReference>
    <dbReference type="GeneID" id="10643587"/>
    <dbReference type="KEGG" id="mig:Metig_0748"/>
    <dbReference type="HOGENOM" id="CLU_021095_10_0_2"/>
    <dbReference type="OMA" id="RMSITND"/>
    <dbReference type="OrthoDB" id="102840at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009307">
        <property type="term" value="P:DNA restriction-modification system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.220.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000055">
        <property type="entry name" value="Restrct_endonuc_typeI_TRD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044946">
        <property type="entry name" value="Restrct_endonuc_typeI_TRD_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01420">
        <property type="entry name" value="Methylase_S"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0680">Restriction system</keyword>
    <feature type="domain" description="Methylase_S" evidence="5">
        <location>
            <begin position="18"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="domain" description="Methylase_S" evidence="5">
        <location>
            <begin position="240"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="402"/>
            <end position="432"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01420"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEF96296.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="439" mass="50255" checksum="B5925BAE6DA29E83" modified="2011-07-27" version="1">MQFYKEENFKDSEIGKIPEDWDVKTLDDISKNMYYGITAKAVGESTILKMIRTTDIKDYKIDWQNLPYCEITEKRKNNISKYFIKKGDILISRAGTVGVSVLVDRDVDNVIFGSYLIKIDLKNDYVNPQFVYYYLQSPYYWNHIKNTSAGSTLKNINLPIIKSLKIPLPSLEEQKAIAKVLSDFDSLIETINKQIETLNKTKKGMMQQLLTGNIRVKIKDGKITFQKENNFKDTEVGKIPEDWEVVRLGDVAYKFISGGTPSTKVPQYWNGDIPWIRSVHITKFYIDKNSIEQYITKEGLENSAAKIVPKGNLIIATRVGIGKSAVNLIDVAINQDLTGIVLNKSKIEPFFLVWYLNSPKITSLLESFSRGTTIKGIPQDYIRKLVIPLPPLEEQKKIAEILSNIDKKIEIKRKEKEQVEKAKKKVMDLLLSGKVRVRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCT8</accession>
    <name>F6BCT8_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Type I site-specific deoxyribonuclease</fullName>
            <ecNumber evidence="3">3.1.21.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">Metig_0752</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Endonucleolytic cleavage of DNA to give random double-stranded fragments with terminal 5'-phosphates, ATP is simultaneously hydrolyzed.</text>
            <dbReference type="EC" id="3.1.21.3"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the HsdR family.</text>
    </comment>
    <dbReference type="EC" id="3.1.21.3" evidence="3"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96299.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0752"/>
    <dbReference type="REBASE" id="36256">
        <property type="entry name" value="Mig5ORF746P"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96299">
        <property type="protein sequence ID" value="AEF96299"/>
        <property type="gene ID" value="Metig_0752"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0752"/>
    <dbReference type="HOGENOM" id="CLU_005762_0_0_2"/>
    <dbReference type="OMA" id="KQYEAGM"/>
    <dbReference type="OrthoDB" id="2473at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009035">
        <property type="term" value="F:type I site-specific deoxyribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009307">
        <property type="term" value="P:DNA restriction-modification system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007409">
        <property type="entry name" value="Restrct_endonuc_type1_HsdR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004473">
        <property type="entry name" value="Restrct_endonuc_typeI_HsdR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040980">
        <property type="entry name" value="SWI2_SNF2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04313">
        <property type="entry name" value="HSDR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18766">
        <property type="entry name" value="SWI2_SNF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00348">
        <property type="entry name" value="hsdR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0540">Nuclease</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0680">Restriction system</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="11">
        <location>
            <begin position="244"/>
            <end position="460"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000851"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012654"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AEF96299.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="1016" mass="120939" checksum="D2ED6D0EE4038B08" modified="2011-07-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCT9</accession>
    <name>F6BCT9_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Peptidase M56 BlaR1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0753</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96300.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798903.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0753"/>
    <dbReference type="EnsemblBacteria" id="AEF96300">
        <property type="protein sequence ID" value="AEF96300"/>
        <property type="gene ID" value="Metig_0753"/>
    </dbReference>
    <dbReference type="GeneID" id="10643592"/>
    <dbReference type="KEGG" id="mig:Metig_0753"/>
    <dbReference type="HOGENOM" id="CLU_2067800_0_0_2"/>
    <dbReference type="OMA" id="KYHINEF"/>
    <dbReference type="OrthoDB" id="138037at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008756">
        <property type="entry name" value="Peptidase_M56"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05569">
        <property type="entry name" value="Peptidase_M56"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase_M56" evidence="1">
        <location>
            <begin position="6"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96300.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="114" mass="13560" checksum="47782E9F0EFD308E" modified="2011-07-27" version="1">MKDRKMLNEILSDTINELNLNDKKTNIKIKIKPLKRKIASISLTNKTIYINKNILPYLSDEEIKFILAHELLHLKYGKYHINEFEEELLFLFPNKEAILTNLINKLHQKNNNKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCW4</accession>
    <name>F6BCW4_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Succinate--CoA ligase [ADP-forming] subunit alpha</fullName>
            <ecNumber evidence="3">6.2.1.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Succinyl-CoA synthetase subunit alpha</fullName>
            <shortName evidence="3">SCS-alpha</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">sucD</name>
        <name evidence="8" type="ordered locus">Metig_0779</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3 6">Succinyl-CoA synthetase functions in the citric acid cycle (TCA), coupling the hydrolysis of succinyl-CoA to the synthesis of either ATP or GTP and thus represents the only step of substrate-level phosphorylation in the TCA. The alpha subunit of the enzyme binds the substrates coenzyme A and phosphate, while succinate binding and nucleotide specificity is provided by the beta subunit.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3 6">
            <text>ATP + CoA + succinate = ADP + phosphate + succinyl-CoA</text>
            <dbReference type="Rhea" id="RHEA:17661"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57292"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.2.1.5"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>acetate + ATP + CoA = acetyl-CoA + ADP + phosphate</text>
            <dbReference type="Rhea" id="RHEA:15081"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.2.1.13"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 6">Carbohydrate metabolism; tricarboxylic acid cycle; succinate from succinyl-CoA (ligase route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 6">Heterotetramer of two alpha and two beta subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 5">Belongs to the succinate/malate CoA ligase alpha subunit family.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.5" evidence="3"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96325.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0779"/>
    <dbReference type="EnsemblBacteria" id="AEF96325">
        <property type="protein sequence ID" value="AEF96325"/>
        <property type="gene ID" value="Metig_0779"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0779"/>
    <dbReference type="HOGENOM" id="CLU_052104_0_0_2"/>
    <dbReference type="OMA" id="VIICITE"/>
    <dbReference type="UniPathway" id="UPA00223">
        <property type="reaction ID" value="UER00999"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043758">
        <property type="term" value="F:acetate-CoA ligase (ADP-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004775">
        <property type="term" value="F:succinate-CoA ligase (ADP-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.261">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01988">
        <property type="entry name" value="Succ_CoA_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017440">
        <property type="entry name" value="Cit_synth/succinyl-CoA_lig_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033847">
        <property type="entry name" value="Citrt_syn/SCS-alpha_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003781">
        <property type="entry name" value="CoA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005810">
        <property type="entry name" value="CoA_lig_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005811">
        <property type="entry name" value="CoA_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016102">
        <property type="entry name" value="Succinyl-CoA_synth-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02629">
        <property type="entry name" value="CoA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00549">
        <property type="entry name" value="Ligase_CoA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001553">
        <property type="entry name" value="SucCS_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00881">
        <property type="entry name" value="CoA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52210">
        <property type="entry name" value="SSF52210"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01019">
        <property type="entry name" value="sucCoAalpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01216">
        <property type="entry name" value="SUCCINYL_COA_LIG_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00399">
        <property type="entry name" value="SUCCINYL_COA_LIG_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5 8" id="KW-0436">Ligase</keyword>
    <keyword evidence="2 3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="CoA_binding" evidence="7">
        <location>
            <begin position="3"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Coenzyme A binding" evidence="3">
        <location>
            <begin position="16"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="region of interest" description="Coenzyme A binding" evidence="3">
        <location>
            <begin position="95"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="active site" description="Tele-phosphohistidine intermediate" evidence="3 4">
        <location>
            <position position="245"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="42"/>
        </location>
        <ligand>
            <name>CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="158"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared with subunit beta</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR001553-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEF96325.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="286" mass="29851" checksum="0357F6580A35A118" modified="2011-07-27" version="1">MILLDENTRAVVQGITGKQGSFHTKKMLECGTNIVAGVTPGKGGMEVHGVPVYDTVCEAVDEHDANASVIFVPAPFAKDAAYEAIDAGLDLIVIITEHIPVHDAIDIVDYAESVGAKIIGPNTPGIASPKVGKLGIIPMSILKEGSVGMVSRSGTLTYEIASQITNAGFGQSTCVGIGGDPVTGLRYVEILEMFEKDKETDAVVMIGEIGGSAEEIAAEYIKKMKKPVIGYIAGQSAPEGKRMGHAGAIIEGGKGTAESKMKALEEAGAYVAKKISDIPKILKEVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCW5</accession>
    <name>F6BCW5_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">DNA topoisomerase 1</fullName>
            <ecNumber evidence="13">5.6.2.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">DNA topoisomerase I</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">topA</name>
        <name evidence="18" type="ordered locus">Metig_0780</name>
    </gene>
    <organism evidence="19">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 13">
            <text>ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.</text>
            <dbReference type="EC" id="5.6.2.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="3 13">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the type IA topoisomerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.1" evidence="13"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96326.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0780"/>
    <dbReference type="EnsemblBacteria" id="AEF96326">
        <property type="protein sequence ID" value="AEF96326"/>
        <property type="gene ID" value="Metig_0780"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0780"/>
    <dbReference type="HOGENOM" id="CLU_002929_5_5_2"/>
    <dbReference type="OMA" id="REYCNIT"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003917">
        <property type="term" value="F:DNA topoisomerase type I (single strand cut, ATP-independent) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006265">
        <property type="term" value="P:DNA topological change"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016539">
        <property type="term" value="P:intein-mediated protein splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00186">
        <property type="entry name" value="TOP1Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03362">
        <property type="entry name" value="TOPRIM_TopoIA_TopoIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.290.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.460.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.28.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00952">
        <property type="entry name" value="Topoisom_1_prok"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003586">
        <property type="entry name" value="Hint_dom_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003587">
        <property type="entry name" value="Hint_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036844">
        <property type="entry name" value="Hint_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027434">
        <property type="entry name" value="Homing_endonucl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006142">
        <property type="entry name" value="INTEIN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030934">
        <property type="entry name" value="Intein_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004042">
        <property type="entry name" value="Intein_endonuc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006141">
        <property type="entry name" value="Intein_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004860">
        <property type="entry name" value="LAGLIDADG_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000380">
        <property type="entry name" value="Topo_IA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003601">
        <property type="entry name" value="Topo_IA_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013497">
        <property type="entry name" value="Topo_IA_cen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013824">
        <property type="entry name" value="Topo_IA_cen_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013825">
        <property type="entry name" value="Topo_IA_cen_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013826">
        <property type="entry name" value="Topo_IA_cen_sub3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023405">
        <property type="entry name" value="Topo_IA_core_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003602">
        <property type="entry name" value="Topo_IA_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013498">
        <property type="entry name" value="Topo_IA_Znf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005739">
        <property type="entry name" value="TopoI_arch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028612">
        <property type="entry name" value="Topoisom_1_IA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006171">
        <property type="entry name" value="TOPRIM_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034144">
        <property type="entry name" value="TOPRIM_TopoIII"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11390">
        <property type="entry name" value="PTHR11390"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14528">
        <property type="entry name" value="LAGLIDADG_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01131">
        <property type="entry name" value="Topoisom_bac"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01751">
        <property type="entry name" value="Toprim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01396">
        <property type="entry name" value="zf-C4_Topoisom"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00379">
        <property type="entry name" value="INTEIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00417">
        <property type="entry name" value="PRTPISMRASEI"/>
    </dbReference>
    <dbReference type="SMART" id="SM00305">
        <property type="entry name" value="HintC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00306">
        <property type="entry name" value="HintN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00437">
        <property type="entry name" value="TOP1Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00436">
        <property type="entry name" value="TOP1Bc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00493">
        <property type="entry name" value="TOPRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51294">
        <property type="entry name" value="SSF51294"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55608">
        <property type="entry name" value="SSF55608"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56712">
        <property type="entry name" value="SSF56712"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01445">
        <property type="entry name" value="intein_Nterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01057">
        <property type="entry name" value="topA_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50818">
        <property type="entry name" value="INTEIN_C_TER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50819">
        <property type="entry name" value="INTEIN_ENDONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50817">
        <property type="entry name" value="INTEIN_N_TER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50880">
        <property type="entry name" value="TOPRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0068">Autocatalytic cleavage</keyword>
    <keyword evidence="14" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="11 13" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="12 13 18" id="KW-0413">Isomerase</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0651">Protein splicing</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 13" id="KW-0799">Topoisomerase</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Toprim" evidence="17">
        <location>
            <begin position="2"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="domain" description="DOD-type homing endonuclease" evidence="16">
        <location>
            <begin position="712"/>
            <end position="850"/>
        </location>
    </feature>
    <feature type="domain" description="INTEIN_C_TER" evidence="15">
        <location>
            <begin position="999"/>
            <end position="1021"/>
        </location>
    </feature>
    <feature type="region of interest" description="Interaction with DNA" evidence="13">
        <location>
            <begin position="192"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="1082"/>
            <end position="1109"/>
        </location>
    </feature>
    <feature type="active site" description="O-(5'-phospho-DNA)-tyrosine intermediate" evidence="13">
        <location>
            <position position="311"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with DNA" evidence="13">
        <location>
            <position position="51"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with DNA" evidence="13">
        <location>
            <position position="163"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with DNA" evidence="13">
        <location>
            <position position="167"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with DNA" evidence="13">
        <location>
            <position position="313"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50818"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="AEF96326.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="1297" mass="149973" checksum="B36954074FCF1DF7" modified="2011-07-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCY5</accession>
    <name>F6BCY5_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="3">ADP-ribose diphosphatase</fullName>
            <ecNumber evidence="3">3.6.1.13</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_0801</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.6.1.13" evidence="3"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96346.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0801"/>
    <dbReference type="EnsemblBacteria" id="AEF96346">
        <property type="protein sequence ID" value="AEF96346"/>
        <property type="gene ID" value="Metig_0801"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0801"/>
    <dbReference type="HOGENOM" id="CLU_037162_20_3_2"/>
    <dbReference type="OMA" id="NRDPRGH"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047631">
        <property type="term" value="F:ADP-ribose diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020476">
        <property type="entry name" value="Nudix_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020084">
        <property type="entry name" value="NUDIX_hydrolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00502">
        <property type="entry name" value="NUDIXFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="SSF55811"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00893">
        <property type="entry name" value="NUDIX_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="2">
        <location>
            <begin position="49"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF96346.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="176" mass="20005" checksum="8D00ED6D83ECB33F" modified="2011-07-27" version="1">MLCDNMTKQNKPNCMCICGKKIALSLKRKRYSKKLLTYRMLKKYNLYPSPALTVDGIVEIDNKILLIKRKKHPFKDFWALPGGFVECGETVEEAVVREVEEETGLKTKIKKLLGVYSNPNRDPRGHTISIVFVLDVVSGEISAGDDAKDAGLFDLNNLPKLAFDHEDIIKDYLMNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-06-02" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>F6BD10</accession>
    <name>F6BD10_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0826</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96371.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798973.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96371">
        <property type="protein sequence ID" value="AEF96371"/>
        <property type="gene ID" value="Metig_0826"/>
    </dbReference>
    <dbReference type="GeneID" id="10643667"/>
    <dbReference type="KEGG" id="mig:Metig_0826"/>
    <dbReference type="HOGENOM" id="CLU_186521_0_0_2"/>
    <dbReference type="OMA" id="EMEIMAS"/>
    <dbReference type="OrthoDB" id="112576at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="16"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96371.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="88" mass="10646" checksum="6A62648453C46A11" modified="2011-07-27" version="1">MPMINIDNKFVLKSMKKVFVEELEEMENELKKLYEKYNINSSKELAFDISEGFITSEEARQDLERMKYLEENIERIRSYLRDINMLSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>F6BD18</accession>
    <name>F6BD18_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_0834</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96379.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798981.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0834"/>
    <dbReference type="EnsemblBacteria" id="AEF96379">
        <property type="protein sequence ID" value="AEF96379"/>
        <property type="gene ID" value="Metig_0834"/>
    </dbReference>
    <dbReference type="GeneID" id="10643675"/>
    <dbReference type="KEGG" id="mig:Metig_0834"/>
    <dbReference type="HOGENOM" id="CLU_067068_1_0_2"/>
    <dbReference type="OMA" id="NSSYCNG"/>
    <dbReference type="OrthoDB" id="29411at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007152">
        <property type="entry name" value="DUF354"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39662">
        <property type="entry name" value="PTHR39662"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04007">
        <property type="entry name" value="DUF354"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005357">
        <property type="entry name" value="UCP005357"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF96379.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="341" mass="38624" checksum="0C51C00F28706040" modified="2011-07-27" version="1">MDVWLDLTNAPHVHYFSQLIKKFEKEGIDYLITTRKSQNLEELLNIYGFEYICVGKHGESLKDKLIYSAERIIELTKIIDKEKPKVAIAKHSVELPRVAFGLSIPVVFIVDNEHAEAQNKLTLPLTDDIIIPIGTKKSKLKKQGGRNFTTFNGTCEVANVNSRLKGILPIDKDILKKLGIDNDNPTIVMRSRPNSSYCNGKKDIIPKIIEKLREKIDCNIVAFPRDEKQRKKYISLNAIVPNTIDAISLLYFADAMVGAGGTMNREAAVLGIPTVSCYPKELLGVDKYLIKMGKMTHMKNVNRIVNYVIENLGTKNKKNKNIELEDPTDLMFEKVCSYLKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>F6BD21</accession>
    <name>F6BD21_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">TrkA-N domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0837</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96382.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798984.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0837"/>
    <dbReference type="EnsemblBacteria" id="AEF96382">
        <property type="protein sequence ID" value="AEF96382"/>
        <property type="gene ID" value="Metig_0837"/>
    </dbReference>
    <dbReference type="GeneID" id="10643678"/>
    <dbReference type="KEGG" id="mig:Metig_0837"/>
    <dbReference type="HOGENOM" id="CLU_046377_1_1_2"/>
    <dbReference type="OMA" id="KCATALM"/>
    <dbReference type="OrthoDB" id="19570at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006813">
        <property type="term" value="P:potassium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001667">
        <property type="entry name" value="DDH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038763">
        <property type="entry name" value="DHH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003156">
        <property type="entry name" value="DHHA1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003148">
        <property type="entry name" value="RCK_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01368">
        <property type="entry name" value="DHH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02272">
        <property type="entry name" value="DHHA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02254">
        <property type="entry name" value="TrkA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64182">
        <property type="entry name" value="SSF64182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51201">
        <property type="entry name" value="RCK_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RCK N-terminal" evidence="1">
        <location>
            <begin position="2"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96382.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="487" mass="54088" checksum="37698305714642E5" modified="2011-07-27" version="1">MILIIGYGSFGRRVVNHAKTIDTVVVVDNKETVFDSVEQVDFKYIVGDATDVEVLKKAGIELADTIVILTNNPEINRKIAELVCELNPKAHKIVRGIPKYPDLYDELPIDRIIYPLESAAREVIKEIEKSKLKRKLFDLKNILIKAKRECLGSNNGEKEGNKEKDIAPLLIVTHNNPDPDAMASAMALKTIASKWDVEADIAYGGNIGFDENKAMINLLGIKMKHIRDVDLSKYCAIAVVDTSSSKNLPFDPETKEIDIVIDHHENGDLTARYMDVQSDVGATATILTEYLRELDISPSRDLATALFYAIASDTNYFKRKVTKKDFDAAGYLQGLIDAKTLQMIENPDMDTETMEVLAKAVLNRKIVKGNIALSYVGEISNRDALPRAADFLLRMEGITTTFVFGIVGDNIHISARTKDLRVDVGDILRKAFDGGGHQAAAAAYIPLGIFKAVSDKESLRKLVEEAIRTKIFEVMGIKEEEKQKTSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>F6BD29</accession>
    <name>F6BD29_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 6">Cell division protein FtsZ</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">ftsZ</name>
        <name evidence="9" type="ordered locus">Metig_0846</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Essential cell division protein that forms a contractile ring structure (Z ring) at the future cell division site. The regulation of the ring assembly controls the timing and the location of cell division. One of the functions of the FtsZ ring is to recruit other cell division proteins to the septum to produce a new cell wall between the dividing cells. Binds GTP and shows GTPase activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer. Polymerizes to form a dynamic ring structure in a strictly GTP-dependent manner. Interacts directly with several other division proteins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
        <text evidence="5">Assembles at midcell at the inner surface of the cytoplasmic membrane.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5 6">Belongs to the FtsZ family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96390.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798992.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0846"/>
    <dbReference type="EnsemblBacteria" id="AEF96390">
        <property type="protein sequence ID" value="AEF96390"/>
        <property type="gene ID" value="Metig_0846"/>
    </dbReference>
    <dbReference type="GeneID" id="10643687"/>
    <dbReference type="KEGG" id="mig:Metig_0846"/>
    <dbReference type="HOGENOM" id="CLU_024865_0_1_2"/>
    <dbReference type="OMA" id="GNPSIGQ"/>
    <dbReference type="OrthoDB" id="41117at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032153">
        <property type="term" value="C:cell division site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000917">
        <property type="term" value="P:division septum assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043093">
        <property type="term" value="P:FtsZ-dependent cytokinesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051258">
        <property type="term" value="P:protein polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02201">
        <property type="entry name" value="FtsZ_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1440">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00909">
        <property type="entry name" value="FtsZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000158">
        <property type="entry name" value="Cell_div_FtsZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020805">
        <property type="entry name" value="Cell_div_FtsZ_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045061">
        <property type="entry name" value="FtsZ/CetZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024757">
        <property type="entry name" value="FtsZ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008280">
        <property type="entry name" value="Tub_FtsZ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037103">
        <property type="entry name" value="Tubulin/FtsZ-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018316">
        <property type="entry name" value="Tubulin/FtsZ_2-layer-sand-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036525">
        <property type="entry name" value="Tubulin/FtsZ_GTPase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003008">
        <property type="entry name" value="Tubulin_FtsZ_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30314">
        <property type="entry name" value="PTHR30314"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12327">
        <property type="entry name" value="FtsZ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00091">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00423">
        <property type="entry name" value="CELLDVISFTSZ"/>
    </dbReference>
    <dbReference type="SMART" id="SM00864">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00865">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52490">
        <property type="entry name" value="SSF52490"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55307">
        <property type="entry name" value="SSF55307"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00065">
        <property type="entry name" value="ftsZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01134">
        <property type="entry name" value="FTSZ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01135">
        <property type="entry name" value="FTSZ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="5 6 9" id="KW-0132">Cell division</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 5 6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2 5 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5 6" id="KW-0717">Septation</keyword>
    <feature type="domain" description="Tubulin" evidence="7">
        <location>
            <begin position="37"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="domain" description="Tubulin_C" evidence="8">
        <location>
            <begin position="230"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="45"/>
            <end position="49"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="132"/>
            <end position="134"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="163"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="167"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="210"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AEF96390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="359" mass="38132" checksum="2C54693137D3D381" modified="2011-07-27" version="1">MKFLKNIIENDDFSYENEPLSDVDKELLELLEQAKARITVVGCGGAGNNTINRLTSEGIEGAKTVAINTDAQQLLKTKADKKILIGRNLTRGLGAGGNPKIGEESAKENAEDIKNELQGADMVFITCGLGGGTGTGSAPVVAEISKKLGALTVAIVTLPFSMEGQIRMQNALEGLNKLKNVADTIVVIPNDKLLEIVPNIPLKTAFKVADEVLINSVRGLVELITKDGLINVDFADVRAVMSNGGMAMIGIGESDSEKRAKEAVMNALNCPLLDVEIDGATGALIHVIGPEDLTLDEAKEVVGTVSERLDPKATIIWGATIDENLENTIQVLLVVTGAKPKSEFGLKQTKKSLIDIPKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>F6BD35</accession>
    <name>F6BD35_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Probable tRNA pseudouridine synthase D</fullName>
            <ecNumber evidence="3">5.4.99.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">tRNA pseudouridine(13) synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="3">tRNA pseudouridylate synthase D</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="3">tRNA-uridine isomerase D</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">truD</name>
        <name evidence="5" type="ordered locus">Metig_0852</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Could be responsible for synthesis of pseudouridine from uracil-13 in transfer RNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>uridine(13) in tRNA = pseudouridine(13) in tRNA</text>
            <dbReference type="Rhea" id="RHEA:42540"/>
            <dbReference type="Rhea" id="RHEA-COMP:10105"/>
            <dbReference type="Rhea" id="RHEA-COMP:10106"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="EC" id="5.4.99.27"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the pseudouridine synthase TruD family.</text>
    </comment>
    <dbReference type="EC" id="5.4.99.27" evidence="3"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96396.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798998.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0852"/>
    <dbReference type="EnsemblBacteria" id="AEF96396">
        <property type="protein sequence ID" value="AEF96396"/>
        <property type="gene ID" value="Metig_0852"/>
    </dbReference>
    <dbReference type="GeneID" id="10643693"/>
    <dbReference type="KEGG" id="mig:Metig_0852"/>
    <dbReference type="HOGENOM" id="CLU_005281_4_1_2"/>
    <dbReference type="OMA" id="NWNGSFI"/>
    <dbReference type="OrthoDB" id="92795at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106029">
        <property type="term" value="F:tRNA pseudouridine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031119">
        <property type="term" value="P:tRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2350.20">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01082">
        <property type="entry name" value="TruD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001656">
        <property type="entry name" value="PsdUridine_synth_TruD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011760">
        <property type="entry name" value="PsdUridine_synth_TruD_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042214">
        <property type="entry name" value="TruD_catalytic"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13326">
        <property type="entry name" value="PTHR13326"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01142">
        <property type="entry name" value="TruD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037016">
        <property type="entry name" value="Pseudouridin_synth_euk_prd"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="SSF55120"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00094">
        <property type="entry name" value="tRNA_TruD_broad"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50984">
        <property type="entry name" value="TRUD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="TRUD" evidence="4">
        <location>
            <begin position="207"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="3">
        <location>
            <position position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEF96396.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="434" mass="51073" checksum="E7B2A43E0DC4F179" modified="2011-07-27" version="1">MKLNIISKQRKKETLKNLKEFRKAFRTNFRKNKIYKMIEKMPLNLNKYLTKYSTGGIIKKYPEDFIVEEITEDGIVLEVGKEIGFKDEENWNGSFIHFTLEKKNWNTLDAIREIAKQTKSKRKNFGFAGTKDKFAITTQRVGCFGVSIEDLKKVNIPNITLRDFQKTNKKLKIGCLWGNRFTIKVRDVEVDKSELDNILKELSKLKYVLNYFGIQRFGSVRPITHVVGKFIVQRDFESAFYAYCGTPLPYDGEKSKEARSLVDEGKFKEALKIYPRIFYYEKRMIKYYLKHRDYKKSFNVLPPQLKSMFVNAYQSYLFNEMINERYKYGFEPLEGDILKDGLPTGALIGYNSKFADGIQGEIERDILEREGIKLEDFKIKGFGNFFGDRRPLIARIYDLEYRIDDDGYVLRFKLEKGTYATSVLREFIDKKINI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F6BD51</accession>
    <name>F6BD51_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Enolase domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0868</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96412.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799014.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0868"/>
    <dbReference type="EnsemblBacteria" id="AEF96412">
        <property type="protein sequence ID" value="AEF96412"/>
        <property type="gene ID" value="Metig_0868"/>
    </dbReference>
    <dbReference type="GeneID" id="10643712"/>
    <dbReference type="KEGG" id="mig:Metig_0868"/>
    <dbReference type="HOGENOM" id="CLU_893150_0_0_2"/>
    <dbReference type="OMA" id="IDSYLWE"/>
    <dbReference type="OrthoDB" id="39216at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029017">
        <property type="entry name" value="Enolase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020811">
        <property type="entry name" value="Enolase_N"/>
    </dbReference>
    <dbReference type="SMART" id="SM01193">
        <property type="entry name" value="Enolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54826">
        <property type="entry name" value="SSF54826"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Enolase_N" evidence="1">
        <location>
            <begin position="7"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM01193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96412.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="312" mass="35034" checksum="3B470483F901E238" modified="2011-07-27" version="1">MENTTIIERITAKKVFKGSKIKVMITTVTNTSIGYDIIDTENPDYVISDVENVIAPELVGYPASNQDFIDSLICNVSVDDTRTTMGVSISVARAAANSMDIPLFKHLGGALITELPIVGCSLLSDKEDNKLIAIPMADSIGEIVVIYDKILNKLSEVYEIKNMHGEFVCKDVFNEINTFKSIVENVKEEEDVEILVGGKVTRYDERINELDYLECDELIEFDGFLCVDGVYEESDFSKINPYEMGTITEMYYYINYILEKGVYPAIFGNNSSFSHIGVGFKVPFLRADIGSNVLNELWNIERSLSNPNVRRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>F6BD75</accession>
    <name>F6BD75_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 8">Adenylate kinase</fullName>
            <shortName evidence="8">AK</shortName>
            <ecNumber evidence="3 8">2.7.4.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7 8">ATP-AMP transphosphorylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">adkA</name>
        <name evidence="9" type="ordered locus">Metig_0893</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>AMP + ATP = 2 ADP</text>
            <dbReference type="Rhea" id="RHEA:12973"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.3"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="8">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the archaeal adenylate kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.3" evidence="3 8"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96436.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799038.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="SMR" id="F6BD75"/>
    <dbReference type="STRING" id="880724.Metig_0893"/>
    <dbReference type="EnsemblBacteria" id="AEF96436">
        <property type="protein sequence ID" value="AEF96436"/>
        <property type="gene ID" value="Metig_0893"/>
    </dbReference>
    <dbReference type="GeneID" id="10643737"/>
    <dbReference type="KEGG" id="mig:Metig_0893"/>
    <dbReference type="HOGENOM" id="CLU_119371_0_0_2"/>
    <dbReference type="OMA" id="HRDEMRK"/>
    <dbReference type="OrthoDB" id="79684at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004017">
        <property type="term" value="F:adenylate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00234">
        <property type="entry name" value="Adenylate_kinase_AdkA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023477">
        <property type="entry name" value="Adenylate_kinase_AdkA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 9" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 9" id="KW-0808">Transferase</keyword>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="10"/>
            <end position="18"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00019926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00033336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AEF96436.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="192" mass="21400" checksum="AB2EC9C9DB905E75" modified="2011-07-27" version="1">MKNKVVVVTGVPGVGGTTLTQKTIEKLKEEGIEYKMVNFGTVMFEVAKEEGLVEDRDQMRKLDPDTQKRIQKLAGRKIAEMAKESNVIVDTHSTVKTPKGYLAGLPIWVLEELNPDIIVIVETSSDEILMRRLGDATRNRDIELTSDIDEHQFMNRCAAMAYGVLTGATVKIIKNRDGLLDKAVEELISVLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2020-06-17" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDE4</accession>
    <name>F6BDE4_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_0962</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96505.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96505">
        <property type="protein sequence ID" value="AEF96505"/>
        <property type="gene ID" value="Metig_0962"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0962"/>
    <dbReference type="HOGENOM" id="CLU_2985687_0_0_2"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF96505.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="57" mass="6321" checksum="D4D0924A2759CA3F" modified="2011-07-27" version="1">MDVVGICATALKITKDIELLIKYNGNFKFLGVGTSFGEIIIKNIDNYILVMIIDSKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDK6</accession>
    <name>F6BDK6_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Zinc metalloprotease</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">Metig_1025</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="7 9">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="7 9">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the peptidase M50B family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96567.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799169.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1025"/>
    <dbReference type="MEROPS" id="M50.006"/>
    <dbReference type="EnsemblBacteria" id="AEF96567">
        <property type="protein sequence ID" value="AEF96567"/>
        <property type="gene ID" value="Metig_1025"/>
    </dbReference>
    <dbReference type="GeneID" id="10643879"/>
    <dbReference type="KEGG" id="mig:Metig_1025"/>
    <dbReference type="HOGENOM" id="CLU_037123_1_1_2"/>
    <dbReference type="OMA" id="IELHITF"/>
    <dbReference type="OrthoDB" id="51079at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008915">
        <property type="entry name" value="Peptidase_M50"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016483">
        <property type="entry name" value="UCP006404_Pept_M50_CBS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02163">
        <property type="entry name" value="Peptidase_M50"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006404">
        <property type="entry name" value="UCP006404_Pept_M50_CBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="SSF54631"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-0129">CBS domain</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="7" id="KW-0645">Protease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="7 9" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="20"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="89"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="123"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="171"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="11">
        <location>
            <begin position="221"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="50"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="49"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="53"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="143"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR006404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR006404-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR006404-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AEF96567.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="334" mass="37353" checksum="6FBA754054C143B1" modified="2011-07-27" version="1">MQSLRIFKVMGIPIELHITFILLLILVYFLWGLGGLILYSLLFTSVVLHELGHSYIAKKYGVKIAKIMLLPIGGVAMMDKIPREGEFKIAIAGPLVSVTLGILLLVFSNYVDFNVSGYPLFKSIGYLNILLGIFNLLPAFPMDGGRILRATLSKKISYIKATKIASTIGQYFAFMMLIFGLLNMNIILILIAIFIYVGATQEYRAAIFDEVFKKIKAKDIMTTKIIHVHPEMSIDEFIDFMLKYRHLGYPVIENGNLVGVVTINDIANVEKSKKIRDVMKNPIIVSPDADVSEILGSMDEEDRVFVVENGNLKGLISKTDVLRALRILGLKEGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDM1</accession>
    <name>F6BDM1_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Coenzyme F420 hydrogenase., Hydrogensulfite reductase</fullName>
            <ecNumber evidence="6">1.12.98.1</ecNumber>
            <ecNumber evidence="6">1.8.99.3</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Metig_1040</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.12.98.1" evidence="6"/>
    <dbReference type="EC" id="1.8.99.3" evidence="6"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96582.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799184.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1040"/>
    <dbReference type="EnsemblBacteria" id="AEF96582">
        <property type="protein sequence ID" value="AEF96582"/>
        <property type="gene ID" value="Metig_1040"/>
    </dbReference>
    <dbReference type="GeneID" id="10643894"/>
    <dbReference type="KEGG" id="mig:Metig_1040"/>
    <dbReference type="HOGENOM" id="CLU_428041_0_0_2"/>
    <dbReference type="OMA" id="GDECWKP"/>
    <dbReference type="OrthoDB" id="49654at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050454">
        <property type="term" value="F:coenzyme F420 hydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018551">
        <property type="term" value="F:hydrogensulfite reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.413.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007516">
        <property type="entry name" value="Co_F420_Hydgase/DH_bsu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045220">
        <property type="entry name" value="FRHB/FDHB/HCAR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007525">
        <property type="entry name" value="FrhB_FdhB_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005117">
        <property type="entry name" value="NiRdtase/SiRdtase_haem-b_fer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036136">
        <property type="entry name" value="Nit/Sulf_reduc_fer-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006067">
        <property type="entry name" value="NO2/SO3_Rdtase_4Fe4S_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045854">
        <property type="entry name" value="NO2/SO3_Rdtase_4Fe4S_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006066">
        <property type="entry name" value="NO2/SO3_Rdtase_FeS/sirohaem_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31332">
        <property type="entry name" value="PTHR31332"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00037">
        <property type="entry name" value="Fer4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12800">
        <property type="entry name" value="Fer4_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04432">
        <property type="entry name" value="FrhB_FdhB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04422">
        <property type="entry name" value="FrhB_FdhB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01077">
        <property type="entry name" value="NIR_SIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03460">
        <property type="entry name" value="NIR_SIR_ferr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00397">
        <property type="entry name" value="SIROHAEM"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55124">
        <property type="entry name" value="SSF55124"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56014">
        <property type="entry name" value="SSF56014"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00365">
        <property type="entry name" value="NIR_SIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0349">Heme</keyword>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="5">
        <location>
            <begin position="6"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="5">
        <location>
            <begin position="486"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="5">
        <location>
            <begin position="520"/>
            <end position="544"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEF96582.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="617" mass="68946" checksum="1DF923E28B553476" modified="2011-07-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDM4</accession>
    <name>F6BDM4_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein pelota homolog</fullName>
            <ecNumber evidence="9">3.1.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">pelA</name>
        <name evidence="11" type="ordered locus">Metig_1044</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">May function in recognizing stalled ribosomes, interact with stem-loop structures in stalled mRNA molecules, and effect endonucleolytic cleavage of the mRNA. May play a role in the release non-functional ribosomes and degradation of damaged mRNAs. Has endoribonuclease activity.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 9">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="9">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="9">The N-terminal domain has the RNA-binding Sm fold. It harbors the endoribonuclease activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the eukaryotic release factor 1 family. Pelota subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="9"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96585.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799187.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1044"/>
    <dbReference type="EnsemblBacteria" id="AEF96585">
        <property type="protein sequence ID" value="AEF96585"/>
        <property type="gene ID" value="Metig_1044"/>
    </dbReference>
    <dbReference type="GeneID" id="10643898"/>
    <dbReference type="KEGG" id="mig:Metig_1044"/>
    <dbReference type="HOGENOM" id="CLU_023334_0_0_2"/>
    <dbReference type="OMA" id="DDLWHLK"/>
    <dbReference type="OrthoDB" id="90096at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070966">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, no-go decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070481">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, non-stop decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071025">
        <property type="term" value="P:RNA surveillance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.870">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01853">
        <property type="entry name" value="PelO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042226">
        <property type="entry name" value="eFR1_2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005140">
        <property type="entry name" value="eRF1_1_Pelota"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005141">
        <property type="entry name" value="eRF1_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005142">
        <property type="entry name" value="eRF1_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029064">
        <property type="entry name" value="L30e-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038069">
        <property type="entry name" value="Pelota/DOM34_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023521">
        <property type="entry name" value="Pelota_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004405">
        <property type="entry name" value="Transl-rel_pelota"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10853">
        <property type="entry name" value="PTHR10853"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03463">
        <property type="entry name" value="eRF1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03464">
        <property type="entry name" value="eRF1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03465">
        <property type="entry name" value="eRF1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01194">
        <property type="entry name" value="eRF1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF159065">
        <property type="entry name" value="SSF159065"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55315">
        <property type="entry name" value="SSF55315"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00111">
        <property type="entry name" value="pelota"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 9" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="8 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 9" id="KW-0540">Nuclease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="eRF1_1" evidence="10">
        <location>
            <begin position="1"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM01194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AEF96585.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="347" mass="39502" checksum="9B55AF6EC957F9D4" modified="2011-07-27" version="1">MKIVQEIPERDIIKVMPENLDDLWYLSTIIERGDNVYAVTERRVQDKGDKLRADRGVKRRMFLGVRCEKVEFHEDTNRLRIFGTIIHGPDDVPLGSHHTIEVEPFTEVSIQKRWKKWHLERLKEAVESSQKPKIVAVVMDDSEADIYLIREFGVKEVASIKSDVSKKLDYKANEQVRAEYYHEIAKTLLNYDTDKILVAGPGFGKNNFQKFASEKYKDLAKKIVVENTCTTGRTGLNEILKSGIINKIYGEARLSKEAQYVNKLLEEIAKNGLAAYGIDEVKKALNYSAIETLLVTDEMLRKRHIEEIMNLTESIGGKVIVISTTHDAGKQLKALGGIAALLRFPIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDN1</accession>
    <name>F6BDN1_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Extracellular solute-binding protein family 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1051</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96592.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799193.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1051"/>
    <dbReference type="EnsemblBacteria" id="AEF96592">
        <property type="protein sequence ID" value="AEF96592"/>
        <property type="gene ID" value="Metig_1051"/>
    </dbReference>
    <dbReference type="GeneID" id="10643905"/>
    <dbReference type="KEGG" id="mig:Metig_1051"/>
    <dbReference type="HOGENOM" id="CLU_065520_2_0_2"/>
    <dbReference type="OMA" id="LAYKYQE"/>
    <dbReference type="OrthoDB" id="57931at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015689">
        <property type="term" value="P:molybdate ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005950">
        <property type="entry name" value="ModA"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004846">
        <property type="entry name" value="ModA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96592.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="280" mass="31658" checksum="80BE6AE95DB0E546" modified="2011-07-27" version="1">MRKLSLLVVGVIVSSLLLCGCVSNQATENQKTLVVYSCGGPTEALQELNSVFEKKYNCKIDFVGASSGTLRKAIENGAYCDVYMPRGVKHAEILLNKSLIEPDFKIYQFTEWVIITPKGNPKNITKLEDLLRDDVKVYTNSKSSIPESRALKPKKDLIEKIYEKSAKDFDCYRKMLEYVVDGECDAALVERRCTTLEGIKGNVEIIEIPREYLNKADGFFTVGVMKNSKNKDLAYKYMEFVLSDEGQSILAKHGFIPVKSEKGQEILNKYYPEYKDLAPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-06-02" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDP6</accession>
    <name>F6BDP6_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1068</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96607.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799208.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96607">
        <property type="protein sequence ID" value="AEF96607"/>
        <property type="gene ID" value="Metig_1068"/>
    </dbReference>
    <dbReference type="GeneID" id="10643922"/>
    <dbReference type="KEGG" id="mig:Metig_1068"/>
    <dbReference type="HOGENOM" id="CLU_2490474_0_0_2"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="30"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="61"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96607.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="86" mass="9963" checksum="0204299C885FC21B" modified="2011-07-27" version="1">MKDKTKLAIFLFLLPLLYFIIYFISKGYFYSILIGISTLALGISIYTESYKQCYKIFGKTFVNIIIILIYLTLLIAGIHFILVHTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDQ0</accession>
    <name>F6BDQ0_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Tetratricopeptide TPR_2 repeat-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1072</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96611.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799212.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1072"/>
    <dbReference type="EnsemblBacteria" id="AEF96611">
        <property type="protein sequence ID" value="AEF96611"/>
        <property type="gene ID" value="Metig_1072"/>
    </dbReference>
    <dbReference type="GeneID" id="10643926"/>
    <dbReference type="KEGG" id="mig:Metig_1072"/>
    <dbReference type="HOGENOM" id="CLU_830580_0_0_2"/>
    <dbReference type="OMA" id="CYWNLVS"/>
    <dbReference type="OrthoDB" id="64283at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006597">
        <property type="entry name" value="Sel1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_repeat"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00671">
        <property type="entry name" value="SEL1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="168"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="202"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="235"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="272"/>
            <end position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96611.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="336" mass="39918" checksum="9EAA6E240EF04E9E" modified="2011-07-27" version="1">MDKKFTLETLNLLKNKVLENDHIFGKELTEILIDVIDGIIYVFNEIFEEISKTNLDNINLRNLWFIYSEISPLYEDINKISSEIDDILNLSSKDINNWKLWKNLGDKAYLCKAYYEALFCYNKALEINSEDLEVLCKKGYTLLRISLNEINLSIKYFEKVLEKDENNYKALFGLGEAYYNLNNEENAIKYFEKILKLNPNDVEALEYLGDIYYEKDYEKAINYYKKALELKPKDVNLILKIAHSYVELKKYEDALKYFEKALSLNPDVFELEQIYEFMGRIYIYLGEDEKAMEYFEKLKEINPYHDEIYEVIALTYGEVGNVEKAEKYLRKLELTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-06-02" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDU9</accession>
    <name>F6BDU9_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1121</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96660.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96660">
        <property type="protein sequence ID" value="AEF96660"/>
        <property type="gene ID" value="Metig_1121"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_1121"/>
    <dbReference type="HOGENOM" id="CLU_2127833_0_0_2"/>
    <dbReference type="OMA" id="ALIVPWF"/>
    <dbReference type="OrthoDB" id="131749at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="52"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="76"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96660.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="113" mass="13668" checksum="F036028D4624D65C" modified="2011-07-27" version="1">MDSWVIVIFLIIYALIVPWFVNWIILNRQNKKVGFLKSFHYLEKKFSTKEKFVIIFVEFILVITLLYGYTRLNISQLYRIFYLFSGILIVFLFNYLLKISLRHAKSKQISFND</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDW0</accession>
    <name>F6BDW0_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">H/ACA RNA-protein complex component Gar1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_1132</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96671.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799272.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1132"/>
    <dbReference type="EnsemblBacteria" id="AEF96671">
        <property type="protein sequence ID" value="AEF96671"/>
        <property type="gene ID" value="Metig_1132"/>
    </dbReference>
    <dbReference type="GeneID" id="10643990"/>
    <dbReference type="KEGG" id="mig:Metig_1132"/>
    <dbReference type="HOGENOM" id="CLU_165884_4_0_2"/>
    <dbReference type="OMA" id="FVMNEQP"/>
    <dbReference type="OrthoDB" id="115856at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038664">
        <property type="entry name" value="Gar1/Naf1_Cbf5-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="SSF50447"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF96671.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="85" mass="9894" checksum="D4A7167A17753D65" modified="2011-07-27" version="1">MKRIIITHKTPKGKLIGRANFQPPINSIVILKSNKKFKKVGKIYDVFGPVNRPYVKIIPFKGFEDYIGEVYILKQKNGKSKYRKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDX4</accession>
    <name>F6BDX4_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Cobyrinic acid ac-diamide synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metig_1146</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96685.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799286.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1146"/>
    <dbReference type="EnsemblBacteria" id="AEF96685">
        <property type="protein sequence ID" value="AEF96685"/>
        <property type="gene ID" value="Metig_1146"/>
    </dbReference>
    <dbReference type="GeneID" id="10644004"/>
    <dbReference type="KEGG" id="mig:Metig_1146"/>
    <dbReference type="HOGENOM" id="CLU_565768_0_0_2"/>
    <dbReference type="OMA" id="FHCHTYG"/>
    <dbReference type="OrthoDB" id="18243at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006541">
        <property type="term" value="P:glutamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011698">
        <property type="entry name" value="GATase_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07685">
        <property type="entry name" value="GATase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="SSF52317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GATase_3" evidence="3">
        <location>
            <begin position="30"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="195"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEF96685.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="487" mass="54888" checksum="ABF521208B32177F" modified="2011-07-27" version="1">MEIGILDIKGSLPCFEYFGNLPTKIIDENNIKDIKDLDMLIIPGGSIVESKSLNENLKKEIVNFDGYIIGICSGFQILSEKIDIGRKSPVPIIREGLGLLNVEFSPLICTDRVKATLETDSILGKKGTVGEGFHCHTYGNIKITDKKTKPLTISEVKKLNYKMQKTCKNIISGAVCGKVIGTMVHGFLDNEHIRNNILDNLKVDEEERKKILEKNKEVMKKLKLRGLKFRNNTNKNITNTENKKEIRGIILLATGSESGKTFLTTAICSKLDGKVLVSKIGPDVRDIVPALYLLREPMLKYSSIKISDRGWCEVSEYLNYLKNSNYDYVIIEGVMGAFTGALNKKNYSGAEIAKLLNFPVYIVSACNKSGIEGAFVESLAYYSLLKEMGIKVEGIILNRVYNFNVFEKVKKIGEEIGVKVIGIRKVEMERRGLIPEVEIDYEEFCKLAMELDIQLEIPKVEIKGINEENKNFEDYLKEWSKKIKNIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>F6BE26</accession>
    <name>F6BE26_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Methyl-viologen-reducing hydrogenase delta subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Metig_1199</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>2Fe-2S cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49601"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MvhD/VhuD family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96737.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1199"/>
    <dbReference type="EnsemblBacteria" id="AEF96737">
        <property type="protein sequence ID" value="AEF96737"/>
        <property type="gene ID" value="Metig_1199"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_1199"/>
    <dbReference type="HOGENOM" id="CLU_095272_2_0_2"/>
    <dbReference type="OMA" id="FPCTGRI"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003813">
        <property type="entry name" value="MvhD/FlpD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02662">
        <property type="entry name" value="FlpD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="5" id="KW-0249">Electron transport</keyword>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="FlpD" evidence="7">
        <location>
            <begin position="18"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEF96737.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="150" mass="17254" checksum="252737F2834BC2B9" modified="2011-07-27" version="1">MDIKIFNGDLMSEWEPKIIGFCCNWCTYGGADTAGVGRMQYPPSIRIIRVMCSGRIEPSLVLKAFREGADGVFVGGCHLGDCHYDSGNYKWQRRVMMLYDLLEELGIERERLRHEWISASEGEKFQQTMIEFYNTIKSLGPCKLKEKLNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>F6BE35</accession>
    <name>F6BE35_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Beta-lactamase domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0009</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95571.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0009"/>
    <dbReference type="EnsemblBacteria" id="AEF95571">
        <property type="protein sequence ID" value="AEF95571"/>
        <property type="gene ID" value="Metig_0009"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0009"/>
    <dbReference type="HOGENOM" id="CLU_074581_0_0_2"/>
    <dbReference type="OMA" id="HCHPDHY"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12706">
        <property type="entry name" value="Lactamase_B_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="SSF56281"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Lactamase_B" evidence="1">
        <location>
            <begin position="68"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF95571.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="304" mass="34166" checksum="C46020EE8CC0E6D4" modified="2011-07-27" version="1">MDFEQICVICKEYVFITFFVILKSNLKNNGCGKLRVEIIFLGSGGGRWATITQTKATGGFRIHTEKTKLHVDPGPGALVRMRELKITPWGTNALFISHCHPDHYTDGEVIIEAMTRGMTQKNGVIISNFTVLEGFGDFENAISKYHQSKVLKRYILYPKDSAKVYDIELIATKTKHGDPYGIGARIKTKKGDIGYTGDTEYIDSLVEDFDGVRVFIANVVRKKNERIRGHLCSNDVIDLVNSMNKKPELIIMNHMGVKMTNPIKEANYIAENTETNVIPAKLGLKIELLEEGYLSYIIGQEKTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F6BE59</accession>
    <name>F6BE59_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Ribosomal L11 methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_0034</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95595.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798204.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0034"/>
    <dbReference type="EnsemblBacteria" id="AEF95595">
        <property type="protein sequence ID" value="AEF95595"/>
        <property type="gene ID" value="Metig_0034"/>
    </dbReference>
    <dbReference type="GeneID" id="10642869"/>
    <dbReference type="KEGG" id="mig:Metig_0034"/>
    <dbReference type="HOGENOM" id="CLU_1067987_0_0_2"/>
    <dbReference type="OMA" id="VPQWHYS"/>
    <dbReference type="OrthoDB" id="77659at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016274">
        <property type="term" value="F:protein-arginine N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018216">
        <property type="term" value="P:peptidyl-arginine methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025799">
        <property type="entry name" value="Arg_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021172">
        <property type="entry name" value="UCP006607_RNA_methylase-rel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11006">
        <property type="entry name" value="PTHR11006"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006607">
        <property type="entry name" value="RNAmts_UCP006607"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF95595.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="260" mass="29401" checksum="8AABC08CCDD8627F" modified="2011-07-27" version="1">MKLKLLVPQWHYSMLLDDERVGIFKEAIERIVKEDDVVYDLGTGSGILAMIAARKAKKVYAVELDPITATYAKKNVKNNNFDNIEVIEADAIEYNFKEKADVVVAEMLDTALITEPQVKVINSILKRGLLKENGKIIPEEAYNTAQIVESRLGHIYYDEEMESRPISDEILYSTINFYEINKEDVSYTLEFEVFENCENPALRLATYTKLTDGLIAGNTPMLNPMLAIPLDKPIKKGKVKVKLSYKMGGDLESIKVSVIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F6BE73</accession>
    <name>F6BE73_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Sulf_transp domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Metig_1212</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96750.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799349.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1212"/>
    <dbReference type="EnsemblBacteria" id="AEF96750">
        <property type="protein sequence ID" value="AEF96750"/>
        <property type="gene ID" value="Metig_1212"/>
    </dbReference>
    <dbReference type="GeneID" id="10644072"/>
    <dbReference type="KEGG" id="mig:Metig_1212"/>
    <dbReference type="HOGENOM" id="CLU_1673998_0_0_2"/>
    <dbReference type="OMA" id="CFCGGIR"/>
    <dbReference type="OrthoDB" id="87630at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007272">
        <property type="entry name" value="Sulf_transp"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04143">
        <property type="entry name" value="Sulf_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="41"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="91"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="131"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="domain" description="Sulf_transp" evidence="7">
        <location>
            <begin position="22"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEF96750.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="157" mass="16826" checksum="8D901EED0B26427D" modified="2011-07-27" version="1">MEYLHAIGTFAFGILLGYLFQRSRMCFVGGMRDFYLIRDTWLIKGLFGFFGGALLGYIIFSAMGKIPFFPWIAVKGFTPIPGDPLGKAGSLVGHLIVAIIGGFGVGFFSVIQGGCPLRNYVMAAEGNKTAIAYVLGLAVGAVLFHTVVVPIVKAILL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>F6BE79</accession>
    <name>F6BE79_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Translin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1219</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96756.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799355.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1219"/>
    <dbReference type="EnsemblBacteria" id="AEF96756">
        <property type="protein sequence ID" value="AEF96756"/>
        <property type="gene ID" value="Metig_1219"/>
    </dbReference>
    <dbReference type="GeneID" id="10644080"/>
    <dbReference type="KEGG" id="mig:Metig_1219"/>
    <dbReference type="HOGENOM" id="CLU_099315_0_0_2"/>
    <dbReference type="OMA" id="EKTHGDI"/>
    <dbReference type="OrthoDB" id="100043at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016070">
        <property type="term" value="P:RNA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033956">
        <property type="entry name" value="Translin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002848">
        <property type="entry name" value="Translin_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036081">
        <property type="entry name" value="Translin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10741">
        <property type="entry name" value="PTHR10741"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10741:SF2">
        <property type="entry name" value="PTHR10741:SF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01997">
        <property type="entry name" value="Translin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74784">
        <property type="entry name" value="SSF74784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="10"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96756.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="200" mass="24418" checksum="1728A6B3155EBD98" modified="2011-07-27" version="1">MFNENEVSYLVKYFEEKDMLREEILKLSREITKDCALSIRKIHKREEVSLEEIFEKLKQLNELVKKHVDFEKYANTPQQEFVEAKALYDIIFNNKIPTYKEFEFIKEENYVLGLCDVIGELRRALLDAIKDDNKELAEKYFNYMEEIYDFIMKFDHYHVIENLRRKQDISRSILEKTHGDLVNYIENLKLREELRKWKEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEA2</accession>
    <name>F6BEA2_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Tetrahydromethanopterin S-methyltransferase subunit A</fullName>
            <ecNumber evidence="4">2.1.1.86</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">N5-methyltetrahydromethanopterin--coenzyme M methyltransferase subunit A</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">mtrA</name>
        <name evidence="5" type="ordered locus">Metig_1242</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Part of a complex that catalyzes the formation of methyl-coenzyme M and tetrahydromethanopterin from coenzyme M and methyl-tetrahydromethanopterin. This is an energy-conserving, sodium-ion translocating step.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>5-methyl-5,6,7,8-tetrahydromethanopterin + coenzyme M + 2 Na(+)(in) = 5,6,7,8-tetrahydromethanopterin + methyl-coenzyme M + 2 Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:53492"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:58103"/>
            <dbReference type="ChEBI" id="CHEBI:58116"/>
            <dbReference type="ChEBI" id="CHEBI:58286"/>
            <dbReference type="ChEBI" id="CHEBI:58319"/>
            <dbReference type="EC" id="2.1.1.86"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>5-hydroxybenzimidazolylcob(I)amide</name>
            <dbReference type="ChEBI" id="CHEBI:60494"/>
        </cofactor>
        <text evidence="4">Binds 1 5-hydroxybenzimidazolylcobamide group.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4">One-carbon metabolism; methanogenesis from CO(2); methyl-coenzyme M from 5,10-methylene-5,6,7,8-tetrahydromethanopterin: step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">The complex is composed of 8 subunits; MtrA, MtrB, MtrC, MtrD, MtrE, MtrF, MtrG and MtrH.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell membrane</location>
            <topology evidence="4">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the MtrA family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.86" evidence="4"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96779.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799378.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1242"/>
    <dbReference type="EnsemblBacteria" id="AEF96779">
        <property type="protein sequence ID" value="AEF96779"/>
        <property type="gene ID" value="Metig_1242"/>
    </dbReference>
    <dbReference type="GeneID" id="10644105"/>
    <dbReference type="KEGG" id="mig:Metig_1242"/>
    <dbReference type="HOGENOM" id="CLU_100863_0_0_2"/>
    <dbReference type="OMA" id="EVKGHIT"/>
    <dbReference type="OrthoDB" id="89197at2157"/>
    <dbReference type="UniPathway" id="UPA00640">
        <property type="reaction ID" value="UER00698"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050897">
        <property type="term" value="F:cobalt ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030269">
        <property type="term" value="F:tetrahydromethanopterin S-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019386">
        <property type="term" value="P:methanogenesis, from carbon dioxide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006814">
        <property type="term" value="P:sodium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01093">
        <property type="entry name" value="MtrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030688">
        <property type="entry name" value="MeTrfase_MtrA/MtxA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005778">
        <property type="entry name" value="MtrA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04208">
        <property type="entry name" value="MtrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF500207">
        <property type="entry name" value="MtrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF009452">
        <property type="entry name" value="MtrA_MtxA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01111">
        <property type="entry name" value="mtrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4" id="KW-0170">Cobalt</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0484">Methanogenesis</keyword>
    <keyword evidence="2 4 5" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="1 4" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4 5" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="222"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="85"/>
        </location>
        <ligand>
            <name>5-hydroxybenzimidazolylcob(I)amide</name>
            <dbReference type="ChEBI" id="CHEBI:60494"/>
            <note>cofactor</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEF96779.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="243" mass="25501" checksum="BD5F2CA1957EEB03" modified="2011-07-27" version="1">MANKKAPAAGWPIVSGEYVVGNPESCVGVVTLGSHGLEQACIDAGAAIAGPCHTENLGIEKVVANYISNPNIRFMVLCGSEVQGHITGQCFKALWENGIGDDGGIIGAKGAIPFLENVGKDAVERFQRQIVEVVDLIDCEDVSKITAAIKECISKDPGAIDEEPMVLDLEGGGAGGEEEGATVKPTSPEIALIEARMRLISEKINDAAILAKYNAGYYNGKVQGIAIGLFLSLLLFSLIHPLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-06-02" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEB7</accession>
    <name>F6BEB7_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized conserved protein UCP037052</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_1257</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96794.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799392.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96794">
        <property type="protein sequence ID" value="AEF96794"/>
        <property type="gene ID" value="Metig_1257"/>
    </dbReference>
    <dbReference type="GeneID" id="10644120"/>
    <dbReference type="KEGG" id="mig:Metig_1257"/>
    <dbReference type="HOGENOM" id="CLU_1529229_0_0_2"/>
    <dbReference type="OMA" id="QWVVLSE"/>
    <dbReference type="OrthoDB" id="78436at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017098">
        <property type="entry name" value="UCP037052"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09869">
        <property type="entry name" value="DUF2096"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037052">
        <property type="entry name" value="UCP037052"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF96794.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="181" mass="21082" checksum="39E702E3ACDDA88D" modified="2011-07-27" version="1">MKDAKGLDKQWVVLSELASKLVEKNIAVPEEAFERLRIANAIISYYLLDPHVSNTALSDAEKELMRVQSLLFSVCDAELMNEYLDKMAKALRNELDVKFPLSKSMFNKEVKKRGDFESVRVKIKNEIQIERLSDLGEWYGVIFEYSEEDENKILIEGNINRVKRALKDFSIIWKFESLEEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>F6BED5</accession>
    <name>F6BED5_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_1275</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96812.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799410.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1275"/>
    <dbReference type="EnsemblBacteria" id="AEF96812">
        <property type="protein sequence ID" value="AEF96812"/>
        <property type="gene ID" value="Metig_1275"/>
    </dbReference>
    <dbReference type="GeneID" id="10644138"/>
    <dbReference type="KEGG" id="mig:Metig_1275"/>
    <dbReference type="HOGENOM" id="CLU_1040553_0_0_2"/>
    <dbReference type="OMA" id="FEPKLWN"/>
    <dbReference type="OrthoDB" id="52801at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF96812.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="267" mass="31281" checksum="0A02E96428571BB0" modified="2011-07-27" version="1">MYIDFKTSFNKLELSEETFKIIDEFGIDLKDIAIGEFSGKDSVAAIIEAFREFDAILPVVAFTGTDYGDKKIFYKNWETLNNRIKNLYKDKVLLPLHFIFEPKLWNALNGRFVSLIIKKFGFYTPCIGCHAYLRILRIPLSKHLGGVIIGGERVYHNSDFKIDQLEMVLEVYEKICDALGVKLKLPIKYVKENSKIREIIEESWKQGKRQFSCVFSGNYRDKDGKIIFEEEKVKRFLDEFIYPVSVEIIKRGYEGDFRYIEIVKEFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEF2</accession>
    <name>F6BEF2_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DNA helicase</fullName>
            <ecNumber evidence="3">3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">Metig_0052</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H2O = ADP + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the HerA family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="3"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95613.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798222.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0052"/>
    <dbReference type="EnsemblBacteria" id="AEF95613">
        <property type="protein sequence ID" value="AEF95613"/>
        <property type="gene ID" value="Metig_0052"/>
    </dbReference>
    <dbReference type="GeneID" id="10642887"/>
    <dbReference type="KEGG" id="mig:Metig_0052"/>
    <dbReference type="HOGENOM" id="CLU_023842_2_0_2"/>
    <dbReference type="OMA" id="CLVSQRP"/>
    <dbReference type="OrthoDB" id="33242at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003678">
        <property type="term" value="F:DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008571">
        <property type="entry name" value="HerA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018538">
        <property type="entry name" value="HerA_barrel_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033186">
        <property type="entry name" value="HerA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002789">
        <property type="entry name" value="HerA_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42957">
        <property type="entry name" value="PTHR42957"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05872">
        <property type="entry name" value="DUF853"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01935">
        <property type="entry name" value="DUF87"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09378">
        <property type="entry name" value="HAS-barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0347">Helicase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HAS-barrel" evidence="10">
        <location>
            <begin position="5"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="domain" description="DUF87" evidence="8">
        <location>
            <begin position="120"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="domain" description="DUF853" evidence="9">
        <location>
            <begin position="341"/>
            <end position="446"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF05872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF09378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AEF95613.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="503" mass="57354" checksum="BA4B708EC61F5CDA" modified="2011-07-27" version="1">MEKIIGYTIGETRTDELTFLAKHPPEVGDYVMISYDGIDVLGMVESTIQGNMVLSEILSIEDLEKIREFDDESSYYIIGKIKVLGDIKELKIPKIPPKPGTPIYKADNETLKNIFSNSHITIGKLATRDIEVSLDVNKLCSRHLAILAMTGMGKSNTVAVLMEELNKIGATILVFDMHGEYRNIESYNFPLRKHVIEPKINIFHISDSDLADLAGVDAQATKQRPYIRKAIKNIKEQYQEKDFNSAEDYINAIIGELEKFMEDDNYKKDKDSIQTAIFRLEDLLQFKKDLIKIHYNPINEIKEHHINILPLESLDENSVDIIVSYFTKEILNDRKRAILENGRDGAKPIFLIFEEAHLIVPQNRKTRAKHYISRIAREGRKFGVGLCLVSQRPKTLDAEALSQCSNLIISKLIEPNDQKHVQHASENLSEDLIKQLTSLNIGEAIIIGPCIKIPAIVKINKFNGHYGGEDIDFIKFWKEKMEKSIKITKEEKEFYYDTSGGLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEF6</accession>
    <name>F6BEF6_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="1 6 9">Phosphoglycerate kinase</fullName>
            <ecNumber evidence="1 6 9">2.7.2.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">pgk</name>
        <name evidence="10" type="ordered locus">Metig_0056</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6 9">
            <text>(2R)-3-phosphoglycerate + ATP = (2R)-3-phospho-glyceroyl phosphate + ADP</text>
            <dbReference type="Rhea" id="RHEA:14801"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57604"/>
            <dbReference type="ChEBI" id="CHEBI:58272"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.2.3"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="6">Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 2/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6 9">Belongs to the phosphoglycerate kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.7.2.3" evidence="1 6 9"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95617.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798226.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0056"/>
    <dbReference type="EnsemblBacteria" id="AEF95617">
        <property type="protein sequence ID" value="AEF95617"/>
        <property type="gene ID" value="Metig_0056"/>
    </dbReference>
    <dbReference type="GeneID" id="10642891"/>
    <dbReference type="KEGG" id="mig:Metig_0056"/>
    <dbReference type="HOGENOM" id="CLU_025427_0_2_2"/>
    <dbReference type="OMA" id="DMIFDIG"/>
    <dbReference type="OrthoDB" id="46017at2157"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00185"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004618">
        <property type="term" value="F:phosphoglycerate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1260">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00145">
        <property type="entry name" value="Phosphoglyc_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001576">
        <property type="entry name" value="Phosphoglycerate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015911">
        <property type="entry name" value="Phosphoglycerate_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015824">
        <property type="entry name" value="Phosphoglycerate_kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036043">
        <property type="entry name" value="Phosphoglycerate_kinase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11406">
        <property type="entry name" value="PTHR11406"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00162">
        <property type="entry name" value="PGK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000724">
        <property type="entry name" value="Pgk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00477">
        <property type="entry name" value="PHGLYCKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53748">
        <property type="entry name" value="SSF53748"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00111">
        <property type="entry name" value="PGLYCERATE_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="4 6 9" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6 9" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Substrate binding" evidence="6 7">
        <location>
            <begin position="20"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="6 7">
        <location>
            <begin position="60"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6 7">
        <location>
            <position position="37"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6 7">
        <location>
            <position position="117"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6 7">
        <location>
            <position position="164"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6 8">
        <location>
            <position position="338"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6 8">
        <location>
            <begin position="364"/>
            <end position="367"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00013061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000724-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000724-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AEF95617.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="416" mass="46128" checksum="A4C7C6B4CC5A8E30" modified="2011-07-27" version="1">MFLTLDDFDLDGKTVLLRVDINSPIDLNTGVVLDDTRIRACKETIEELCNKNARVVILAHQSRPGKKDFTTLEMHAEKLSEVLDREVRYIDDIFGSYARNAIKNMKDGEIILLENVRFLAEEVLKDWKKWKDITPKKQAETHLVKKLYPLCDYFVNDAFAAAHRAQPSLVGFAYYLPMIAGRIMEKELSILGKVLKNPERPCIFVLGGAKADDSIKVMKNVLKNGSADKVLTTGIVANIFLIAKGYKLGVNEKIIEDMGLKDQIGIAKELLDKYEDKIITPIDAALNIDGERKEIDLDMDIEVEYPIHDIGEKTIELYNEIIKDAKTIVANGPAGVFENKNFLKGTAEILNSIANSNAFSVIGGGHLSAAAELIGVADKIGHISTGGGACLEFLAGEELPVITMLEKSYQKYKGQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEM6</accession>
    <name>F6BEM6_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Branched-chain-amino-acid aminotransferase</fullName>
            <shortName evidence="14">BCAT</shortName>
            <ecNumber evidence="14">2.6.1.42</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">ilvE</name>
        <name evidence="15" type="ordered locus">Metig_1286</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5 14">Acts on leucine, isoleucine and valine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 14">
            <text>2-oxoglutarate + L-isoleucine = (S)-3-methyl-2-oxopentanoate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:24801"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:35146"/>
            <dbReference type="ChEBI" id="CHEBI:58045"/>
            <dbReference type="EC" id="2.6.1.42"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 14">
            <text>2-oxoglutarate + L-leucine = 4-methyl-2-oxopentanoate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:18321"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:17865"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57427"/>
            <dbReference type="EC" id="2.6.1.42"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3 14">
            <text>2-oxoglutarate + L-valine = 3-methyl-2-oxobutanoate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:24813"/>
            <dbReference type="ChEBI" id="CHEBI:11851"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57762"/>
            <dbReference type="EC" id="2.6.1.42"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4 14">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="6 14">Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 4/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="8 14">Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 4/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="7 14">Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 4/4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9 13">Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.42" evidence="14"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96823.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799420.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1286"/>
    <dbReference type="EnsemblBacteria" id="AEF96823">
        <property type="protein sequence ID" value="AEF96823"/>
        <property type="gene ID" value="Metig_1286"/>
    </dbReference>
    <dbReference type="GeneID" id="10644149"/>
    <dbReference type="KEGG" id="mig:Metig_1286"/>
    <dbReference type="HOGENOM" id="CLU_020844_3_0_2"/>
    <dbReference type="OMA" id="WRGSEMM"/>
    <dbReference type="OrthoDB" id="41340at2157"/>
    <dbReference type="UniPathway" id="UPA00047">
        <property type="reaction ID" value="UER00058"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00048">
        <property type="reaction ID" value="UER00073"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00049">
        <property type="reaction ID" value="UER00062"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052656">
        <property type="term" value="F:L-isoleucine transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052654">
        <property type="term" value="F:L-leucine transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050048">
        <property type="term" value="F:L-leucine:2-oxoglutarate aminotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052655">
        <property type="term" value="F:L-valine transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009097">
        <property type="term" value="P:isoleucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009098">
        <property type="term" value="P:leucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009099">
        <property type="term" value="P:valine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001544">
        <property type="entry name" value="Aminotrans_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018300">
        <property type="entry name" value="Aminotrans_IV_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036038">
        <property type="entry name" value="Aminotransferase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005785">
        <property type="entry name" value="B_amino_transI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043132">
        <property type="entry name" value="BCAT-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043131">
        <property type="entry name" value="BCAT-like_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01063">
        <property type="entry name" value="Aminotran_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56752">
        <property type="entry name" value="SSF56752"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01122">
        <property type="entry name" value="ilvE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00770">
        <property type="entry name" value="AA_TRANSFER_CLASS_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 14" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="14 15" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="12 14" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="11 14" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="14 15" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000627"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00000995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00003109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00004824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00004931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00005072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00009320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU004106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU364094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="AEF96823.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="287" mass="31976" checksum="87557A30C02F91A1" modified="2011-07-27" version="1">MKVYLNGKFVDKEDAKISVYDHGLLYGDGVFEGIRAYDGVVFKLKEHIDRLYDSAKSICLKIPMSKEEMEKVVIETLRVNNLRDAYIRLVVTRGVGDLGLDPRKCKNPTIFCIAEPMKPLLGEDGIRVITASVRRLPVDVLNPAVKSLNYLNSILAKIQANYAGVDEAFLLDAEGYVAEGTGDNIFVVKNGVIKTPPVSSSVLRGITRDTVIDLAKELGYEVIEERLTLHELYVADEVFITGTAAEIVPVIEIDGRKINDGKIGEITKKLREKFKEVRTKLGTKIYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEN3</accession>
    <name>F6BEN3_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">5,10-methylenetetrahydromethanopterin reductase</fullName>
            <ecNumber evidence="4">1.5.98.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Coenzyme F420-dependent N(5),N(10)-methylenetetrahydromethanopterin reductase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">Methylene-H(4)MPT reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">mer</name>
        <name evidence="6" type="ordered locus">Metig_1293</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Catalyzes the reversible reduction of methylene-H(4)MPT to methyl-H(4)MPT.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>5-methyl-5,6,7,8-tetrahydromethanopterin + H(+) + oxidized coenzyme F420-(gamma-L-Glu)(n) = 5,10-methylenetetrahydromethanopterin + reduced coenzyme F420-(gamma-L-Glu)(n)</text>
            <dbReference type="Rhea" id="RHEA:21144"/>
            <dbReference type="Rhea" id="RHEA-COMP:12939"/>
            <dbReference type="Rhea" id="RHEA-COMP:14378"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57818"/>
            <dbReference type="ChEBI" id="CHEBI:58116"/>
            <dbReference type="ChEBI" id="CHEBI:133980"/>
            <dbReference type="ChEBI" id="CHEBI:139511"/>
            <dbReference type="EC" id="1.5.98.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">One-carbon metabolism; methanogenesis from CO(2); methyl-coenzyme M from 5,10-methylene-5,6,7,8-tetrahydromethanopterin: step 1/2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the mer family.</text>
    </comment>
    <dbReference type="EC" id="1.5.98.2" evidence="4"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96830.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799427.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1293"/>
    <dbReference type="EnsemblBacteria" id="AEF96830">
        <property type="protein sequence ID" value="AEF96830"/>
        <property type="gene ID" value="Metig_1293"/>
    </dbReference>
    <dbReference type="GeneID" id="10644156"/>
    <dbReference type="KEGG" id="mig:Metig_1293"/>
    <dbReference type="HOGENOM" id="CLU_027853_5_3_2"/>
    <dbReference type="OMA" id="VCWAAEA"/>
    <dbReference type="OrthoDB" id="46136at2157"/>
    <dbReference type="UniPathway" id="UPA00640">
        <property type="reaction ID" value="UER00697"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018537">
        <property type="term" value="F:coenzyme F420-dependent N5,N10-methenyltetrahydromethanopterin reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019386">
        <property type="term" value="P:methanogenesis, from carbon dioxide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01091">
        <property type="entry name" value="F420_mer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011251">
        <property type="entry name" value="Luciferase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036661">
        <property type="entry name" value="Luciferase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019946">
        <property type="entry name" value="MeH4methanopterin_reductase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00296">
        <property type="entry name" value="Bac_luciferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51679">
        <property type="entry name" value="SSF51679"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03555">
        <property type="entry name" value="F420_mer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0484">Methanogenesis</keyword>
    <keyword evidence="2 4" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="3 4 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Bac_luciferase" evidence="5">
        <location>
            <begin position="12"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEF96830.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="331" mass="35222" checksum="34ACF865FB49D697" modified="2011-07-27" version="1">MKFGIEFVPNEPIQKLCYYVKLAEDNGFEYCWITDHYNNRNVYMALTAIAAATNKIKLGPGVTNPYVRNPAITASAIATLDELSGGRAVLGIGPGDKATFDALGIEWVKPVTTLKESIEVMRKLLAGERVSFDGNVVKVAGAALAVKPIQKKVPVYVGAQGPKMLETAGMIADGVLINASNPKDFEAAIPLIKKGAEAAGRSMDEIDVAAYACMSVDKNAEKAKQAAVPVVAFIAAGSPPVVLERHGIDMEKVEKIREALKKGDFGTAFGTVDDAMLEAFSIYGTPEEVVEKCKKLAEMGVTQIVAGSPIGPNKETAIKLIGKKVIPALKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEN7</accession>
    <name>F6BEN7_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">NGG1p interacting factor 3 protein, NIF3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1297</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GTP cyclohydrolase I type 2/NIF3 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96834.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799431.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1297"/>
    <dbReference type="EnsemblBacteria" id="AEF96834">
        <property type="protein sequence ID" value="AEF96834"/>
        <property type="gene ID" value="Metig_1297"/>
    </dbReference>
    <dbReference type="GeneID" id="10644160"/>
    <dbReference type="KEGG" id="mig:Metig_1297"/>
    <dbReference type="HOGENOM" id="CLU_037423_2_0_2"/>
    <dbReference type="OMA" id="THHSKIL"/>
    <dbReference type="OrthoDB" id="40682at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002678">
        <property type="entry name" value="DUF34/NIF3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036069">
        <property type="entry name" value="DUF34/NIF3_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13799">
        <property type="entry name" value="PTHR13799"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01784">
        <property type="entry name" value="NIF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102705">
        <property type="entry name" value="SSF102705"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00486">
        <property type="entry name" value="YbgI_SA1388"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96834.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="243" mass="27121" checksum="605C4A8EF67D9477" modified="2011-07-27" version="1">MKAYEIIEIIENFAPKELAIVGDNVGLQVGDNLDKEVNKLGIALDPSLNVIKKAKKEGVDFLFTHHPILKNPIKNFTGIMYEKLKILMENDIILYSAHTNLDICKDGLNDALAELYKLKDVENLYENGLGRVGIFEGSFEELLDITKKYIVESPVVVGNIDGDNFKLAVLSGYGLSQDAIRYVAEKADVYLSGDLTHHSKILAEELGLVVVDGTHYGTEVYGLRKFMDYLKDMVDCEVVSLDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEQ6</accession>
    <name>F6BEQ6_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Putative (R)-citramalate synthase CimA</fullName>
            <ecNumber evidence="5">2.3.1.182</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">cimA</name>
        <name evidence="8" type="ordered locus">Metig_1316</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the condensation of pyruvate and acetyl-coenzyme A to form (R)-citramalate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>acetyl-CoA + H2O + pyruvate = (3R)-citramalate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19045"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30934"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="EC" id="2.3.1.182"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="5">Amino-acid biosynthesis; L-isoleucine biosynthesis; 2-oxobutanoate from pyruvate: step 1/3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5 6">Belongs to the alpha-IPM synthase/homocitrate synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.182" evidence="5"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96853.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799450.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1316"/>
    <dbReference type="EnsemblBacteria" id="AEF96853">
        <property type="protein sequence ID" value="AEF96853"/>
        <property type="gene ID" value="Metig_1316"/>
    </dbReference>
    <dbReference type="GeneID" id="10644179"/>
    <dbReference type="KEGG" id="mig:Metig_1316"/>
    <dbReference type="HOGENOM" id="CLU_022158_0_1_2"/>
    <dbReference type="OMA" id="SNMFAHE"/>
    <dbReference type="OrthoDB" id="10632at2157"/>
    <dbReference type="UniPathway" id="UPA00047">
        <property type="reaction ID" value="UER00066"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043714">
        <property type="term" value="F:(R)-citramalate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003852">
        <property type="term" value="F:2-isopropylmalate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009097">
        <property type="term" value="P:isoleucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009098">
        <property type="term" value="P:leucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01028">
        <property type="entry name" value="CimA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013709">
        <property type="entry name" value="2-isopropylmalate_synth_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002034">
        <property type="entry name" value="AIPM/Hcit_synth_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024890">
        <property type="entry name" value="Citramalate_synthase_CimA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011830">
        <property type="entry name" value="LEU1_arch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036230">
        <property type="entry name" value="LeuA_allosteric_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000891">
        <property type="entry name" value="PYR_CT"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00682">
        <property type="entry name" value="HMGL-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08502">
        <property type="entry name" value="LeuA_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00917">
        <property type="entry name" value="LeuA_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110921">
        <property type="entry name" value="SSF110921"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02090">
        <property type="entry name" value="LEU1_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00815">
        <property type="entry name" value="AIPM_HOMOCIT_SYNTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00816">
        <property type="entry name" value="AIPM_HOMOCIT_SYNTH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50991">
        <property type="entry name" value="PYR_CT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="4 5" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="2 5" id="KW-0412">Isoleucine biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Pyruvate carboxyltransferase" evidence="7">
        <location>
            <begin position="3"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEF96853.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="490" mass="53503" checksum="A324EB64CF7AC1A0" modified="2011-07-27" version="1">MKVRIFDTTLRDGEQTPGVSLTPNEKLEIALKLDELGVDVIEAGSAITSKGEREGIKLITRENLNAEICSFVRALPIDIDAALECDVDSVHLVIPTSDIHMKYKLKKTPQETLECAANAVEYAKDHGLIVELSAEDATRSDVNFLIELFNKGIELGADRVCVCDTVGVLTPEKSIELFKTLKEHIKVPISVHCHNDFGMATANTVCAIRGGAVQCHVTVNGVGERAGNASLEEVVMALQVLYGYETNIKTEKLYETSRVVSRLMKLPVPPNKAIVGDNAFAHEAGIHVDGLIKNTQTYEPIQPELVGNRRRIILGKHSGRAALKYKLNLMGIEVSEEQLNEIYEKVKHFGDLGKYISDIDLKAIVNEVTGKHAEEKIKLDELTVVSGNKITPIASVKLHFAGEDKHLIETSYGVGPVDAAINAVKKAISGVADIRLEEYRVEAMSGGTDALIEVVVKLRRGSEVVEVRKSHGDIIMASVMAVMEGINMLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEW5</accession>
    <name>F6BEW5_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Type-2 serine--tRNA ligase</fullName>
            <ecNumber evidence="11">6.1.1.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Seryl-tRNA synthetase</fullName>
            <shortName evidence="11">SerRS</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Seryl-tRNA(Ser/Sec) synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">serS</name>
        <name evidence="14" type="ordered locus">Metig_0141</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes the attachment of serine to tRNA(Ser). Is also able to aminoacylate tRNA(Sec) with serine, to form the misacylated tRNA L-seryl-tRNA(Sec), which will be further converted into selenocysteinyl-tRNA(Sec).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 11">
            <text>ATP + L-serine + tRNA(Sec) = AMP + diphosphate + H(+) + L-seryl-tRNA(Sec)</text>
            <dbReference type="Rhea" id="RHEA:42580"/>
            <dbReference type="Rhea" id="RHEA-COMP:9742"/>
            <dbReference type="Rhea" id="RHEA-COMP:10128"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78533"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.11"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 11">
            <text>ATP + L-serine + tRNA(Ser) = AMP + diphosphate + H(+) + L-seryl-tRNA(Ser)</text>
            <dbReference type="Rhea" id="RHEA:12292"/>
            <dbReference type="Rhea" id="RHEA-COMP:9669"/>
            <dbReference type="Rhea" id="RHEA-COMP:9703"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78533"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="11">Binds 1 Zn(2+) ion per subunit. This ion is coordinated with 2 cysteines, 1 glutamate and a water molecule that dissociates from the zinc ion to allow the coordination of the amino group of the serine substrate, which is essential for catalysis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4 11">Aminoacyl-tRNA biosynthesis; selenocysteinyl-tRNA(Sec) biosynthesis; L-seryl-tRNA(Sec) from L-serine and tRNA(Sec): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6 11">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3 11">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="11">Consists of two distinct domains, a catalytic core and a N-terminal extension that is presumably involved in tRNA binding.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5 11">Belongs to the class-II aminoacyl-tRNA synthetase family. Type-2 seryl-tRNA synthetase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.11" evidence="11"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95701.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798310.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0141"/>
    <dbReference type="EnsemblBacteria" id="AEF95701">
        <property type="protein sequence ID" value="AEF95701"/>
        <property type="gene ID" value="Metig_0141"/>
    </dbReference>
    <dbReference type="GeneID" id="10642976"/>
    <dbReference type="KEGG" id="mig:Metig_0141"/>
    <dbReference type="HOGENOM" id="CLU_542524_0_0_2"/>
    <dbReference type="OMA" id="YYVCPPK"/>
    <dbReference type="OrthoDB" id="9543at2157"/>
    <dbReference type="UniPathway" id="UPA00906">
        <property type="reaction ID" value="UER00895"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004828">
        <property type="term" value="F:serine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016260">
        <property type="term" value="P:selenocysteine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097056">
        <property type="term" value="P:selenocysteinyl-tRNA(Sec) biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006434">
        <property type="term" value="P:seryl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01278">
        <property type="entry name" value="Ser_tRNA_synth_type2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002314">
        <property type="entry name" value="aa-tRNA-synt_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004503">
        <property type="entry name" value="Ser-tRNA-ligase_2_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041293">
        <property type="entry name" value="SerS_tRNA-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00587">
        <property type="entry name" value="tRNA-synt_2b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18490">
        <property type="entry name" value="tRNA_bind_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="SSF55681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00415">
        <property type="entry name" value="serS_MJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 11 14" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="9 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 11 14" id="KW-0436">Ligase</keyword>
    <keyword evidence="11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="tRNA_bind_4" evidence="13">
        <location>
            <begin position="1"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="domain" description="tRNA-synt_2b" evidence="12">
        <location>
            <begin position="306"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="region of interest" description="Serine binding" evidence="11">
        <location>
            <begin position="360"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="312"/>
        </location>
        <ligand>
            <name>L-serine</name>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="314"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="343"/>
            <end position="345"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="343"/>
        </location>
        <ligand>
            <name>L-serine</name>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="354"/>
            <end position="355"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="362"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="469"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="476"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00005951"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00587"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF18490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="AEF95701.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="513" mass="60032" checksum="C5D953C67DBA9168" modified="2011-07-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEY8</accession>
    <name>F6BEY8_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">5'-deoxyadenosine deaminase</fullName>
            <shortName evidence="1">5'-dA deaminase</shortName>
            <ecNumber evidence="1">3.5.4.41</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">5'-methylthioadenosine deaminase</fullName>
            <shortName evidence="1">MTA deaminase</shortName>
            <ecNumber evidence="1">3.5.4.31</ecNumber>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Adenosine deaminase</fullName>
            <ecNumber evidence="1">3.5.4.4</ecNumber>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">S-adenosylhomocysteine deaminase</fullName>
            <shortName evidence="1">SAH deaminase</shortName>
            <ecNumber evidence="1">3.5.4.28</ecNumber>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">dadD</name>
        <name evidence="3" type="ordered locus">Metig_0166</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the deamination of three SAM-derived enzymatic products, namely 5'-deoxyadenosine, S-adenosyl-L-homocysteine, and 5'-methylthioadenosine, to produce the inosine analogs. Can also deaminate adenosine. The preferred substrate for this enzyme is 5'-deoxyadenosine, but all these substrates are efficiently deaminated. Likely functions in a S-adenosyl-L-methionine (SAM) recycling pathway from S-adenosyl-L-homocysteine (SAH) produced from SAM-dependent methylation reactions. May also be involved in the recycling of 5'-deoxyadenosine, whereupon the 5'-deoxyribose moiety of 5'-deoxyinosine is further metabolized to deoxyhexoses used for the biosynthesis of aromatic amino acids in methanogens.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>5'-deoxyadenosine + H(+) + H2O = 5'-deoxyinosine + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:42892"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:82775"/>
            <dbReference type="EC" id="3.5.4.41"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>H(+) + H2O + S-adenosyl-L-homocysteine = NH4(+) + S-inosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:20716"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:57985"/>
            <dbReference type="EC" id="3.5.4.28"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>H(+) + H2O + S-methyl-5'-thioadenosine = NH4(+) + S-methyl-5'-thioinosine</text>
            <dbReference type="Rhea" id="RHEA:25025"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17509"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:48595"/>
            <dbReference type="EC" id="3.5.4.31"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>adenosine + H(+) + H2O = inosine + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:24408"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16335"/>
            <dbReference type="ChEBI" id="CHEBI:17596"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="EC" id="3.5.4.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; S-adenosyl-L-methionine biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homotetramer.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="1">SAH is a product of SAM methyltransferases and is known to be a feedback inhibitor of these enzymes. As a result of this inhibition, organisms have evolved efficient enzymes to metabolize SAH via different pathways. The pathway found in methanogens differs from the canonical pathway, it uses the deamination of S-adenosyl-L-homocysteine to form S-inosyl-L-homocysteine for the regeneration of SAM from S-adenosyl-L-homocysteine. 5'-deoxyadenosine is a radical SAM enzyme reaction product which strongly inhibits radical SAM enzymes. A pathway for removing this product must be present in methanogens where the MTA/SAH nucleosidase which normally metabolizes this compound is absent.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the metallo-dependent hydrolases superfamily. MTA/SAH deaminase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="1">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.41" evidence="1"/>
    <dbReference type="EC" id="3.5.4.31" evidence="1"/>
    <dbReference type="EC" id="3.5.4.4" evidence="1"/>
    <dbReference type="EC" id="3.5.4.28" evidence="1"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95724.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798333.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0166"/>
    <dbReference type="EnsemblBacteria" id="AEF95724">
        <property type="protein sequence ID" value="AEF95724"/>
        <property type="gene ID" value="Metig_0166"/>
    </dbReference>
    <dbReference type="GeneID" id="10643001"/>
    <dbReference type="KEGG" id="mig:Metig_0166"/>
    <dbReference type="HOGENOM" id="CLU_012358_2_1_2"/>
    <dbReference type="OMA" id="ALIGKVC"/>
    <dbReference type="OrthoDB" id="40873at2157"/>
    <dbReference type="UniPathway" id="UPA00315"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090613">
        <property type="term" value="F:5'-deoxyadenosine deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090614">
        <property type="term" value="F:5'-methylthioadenosine deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004000">
        <property type="term" value="F:adenosine deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050270">
        <property type="term" value="F:S-adenosylhomocysteine deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006556">
        <property type="term" value="P:S-adenosylmethionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01281">
        <property type="entry name" value="MTA_SAH_deamin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006680">
        <property type="entry name" value="Amidohydro-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023512">
        <property type="entry name" value="Deaminase_MtaD/DadD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011059">
        <property type="entry name" value="Metal-dep_hydrolase_composite"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01979">
        <property type="entry name" value="Amidohydro_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51338">
        <property type="entry name" value="SSF51338"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="SSF51556"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Amidohydro-rel" evidence="2">
        <location>
            <begin position="53"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="62"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="64"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="91"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="183"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="210"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="213"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF95724.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="427" mass="48100" checksum="9B5726D6F9645FC8" modified="2011-07-27" version="1">MGDFTYCLIKNAVINGKKQDLLIEKNTIKKIGNIENNEIDKEETKIINAENKIAIPGLINTHTHIPMTLFRGVADDLPLMEWLNNYIWPMEAKLNKDIVYAGTLLGCLEMIKSGTTTFNDMYFFLDGIAKAVDEIGIRAVLSYGMIDLFDEEKREKELKNAEENIKMIKKLDNNRIKVALGPHAPYTCSKELLMEVHEMAKKYNIPIHIHMNETLDEIKMVKEKTGMRPFEYLNSFGFFDDVNVIAAHCVHLSDEEIKIMKEKNINVSHNPISNLKLASGIAPVPKLVENGINVTLGTDGCGSNNNLNLFEEIKISSLLHKGTTLNPTIINAKQSFEFATKNGAKALGLKCGELKEGYLADIVLIDLNKPFLIPKENIYSHLVYSFNGNVDTVIIDGNVVMENGKIVNVDEEEIYEKAEKAYYKLIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEZ7</accession>
    <name>F6BEZ7_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Glucose-1-phosphate thymidylyltransferase</fullName>
            <ecNumber evidence="4">2.7.7.24</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Metig_0176</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>alpha-D-glucose 1-phosphate + dTTP + H(+) = diphosphate + dTDP-alpha-D-glucose</text>
            <dbReference type="Rhea" id="RHEA:15225"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37568"/>
            <dbReference type="ChEBI" id="CHEBI:57477"/>
            <dbReference type="ChEBI" id="CHEBI:58601"/>
            <dbReference type="EC" id="2.7.7.24"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the glucose-1-phosphate thymidylyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.24" evidence="4"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95733.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0176"/>
    <dbReference type="EnsemblBacteria" id="AEF95733">
        <property type="protein sequence ID" value="AEF95733"/>
        <property type="gene ID" value="Metig_0176"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0176"/>
    <dbReference type="HOGENOM" id="CLU_029499_9_0_2"/>
    <dbReference type="OMA" id="RRNWIDA"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008879">
        <property type="term" value="F:glucose-1-phosphate thymidylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045226">
        <property type="term" value="P:extracellular polysaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02538">
        <property type="entry name" value="G1P_TT_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005907">
        <property type="entry name" value="G1P_thy_trans_s"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005835">
        <property type="entry name" value="NTP_transferase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43532">
        <property type="entry name" value="PTHR43532"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00483">
        <property type="entry name" value="NTP_transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01207">
        <property type="entry name" value="rmlA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="NTP_transferase" evidence="5">
        <location>
            <begin position="5"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEF95733.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="295" mass="33357" checksum="41A81DA92EA376DD" modified="2011-07-27" version="1">MITVKGIILAGGSGTRLYPITYAGNKHLMPIYNKPMVYYPLSILMLAKIREILIISTPEDLPRFKKLLGTGEKFGIELYYKEQKEPKGLAEAFIIGEEFIGDDNVCLVLGDNILYGSGLTGFLLEAKEELEKKGGGIIFGQYVKDPERYGVIEFDENGNVKSIIEKPKNPPSNYAVIGLYFYDNEVIEIAKQVKPSWRGELEITDVNNEYLKKRKLKVKLLPRGIAWFDAGTHESFLEATNFVAAIERRQGLMIGCLEEIAYRNGWISKEQLLELAKPLMKTDYGKYLERLANEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>F6BF00</accession>
    <name>F6BF00_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">dTDP-4-dehydrorhamnose 3,5-epimerase</fullName>
            <ecNumber evidence="3">5.1.3.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Thymidine diphospho-4-keto-rhamnose 3,5-epimerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metig_0179</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the epimerization of the C3' and C5'positions of dTDP-6-deoxy-D-xylo-4-hexulose, forming dTDP-6-deoxy-L-lyxo-4-hexulose.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>dTDP-4-dehydro-6-deoxy-alpha-D-glucose = dTDP-4-dehydro-beta-L-rhamnose</text>
            <dbReference type="Rhea" id="RHEA:16969"/>
            <dbReference type="ChEBI" id="CHEBI:57649"/>
            <dbReference type="ChEBI" id="CHEBI:62830"/>
            <dbReference type="EC" id="5.1.3.13"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Carbohydrate biosynthesis; dTDP-L-rhamnose biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the dTDP-4-dehydrorhamnose 3,5-epimerase family.</text>
    </comment>
    <dbReference type="EC" id="5.1.3.13" evidence="3"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95736.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798345.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0179"/>
    <dbReference type="EnsemblBacteria" id="AEF95736">
        <property type="protein sequence ID" value="AEF95736"/>
        <property type="gene ID" value="Metig_0179"/>
    </dbReference>
    <dbReference type="GeneID" id="10643014"/>
    <dbReference type="KEGG" id="mig:Metig_0179"/>
    <dbReference type="HOGENOM" id="CLU_090940_1_1_2"/>
    <dbReference type="OMA" id="RGVFLEW"/>
    <dbReference type="OrthoDB" id="96034at2157"/>
    <dbReference type="UniPathway" id="UPA00124"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008830">
        <property type="term" value="F:dTDP-4-dehydrorhamnose 3,5-epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019305">
        <property type="term" value="P:dTDP-rhamnose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000888">
        <property type="entry name" value="dTDP_sugar_isom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21047">
        <property type="entry name" value="PTHR21047"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00908">
        <property type="entry name" value="dTDP_sugar_isom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="SSF51182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01221">
        <property type="entry name" value="rmlC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0413">Isomerase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="63"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="1">
        <location>
            <position position="133"/>
        </location>
    </feature>
    <feature type="site" description="Participates in a stacking interaction with the thymidine ring of dTDP-4-oxo-6-deoxyglucose" evidence="2">
        <location>
            <position position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR600888-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR600888-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU364069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEF95736.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="185" mass="21586" checksum="09F467E2BD5E614E" modified="2011-07-27" version="1">MPFEFIKTKIPDVILIKPKVFGDERGFFMETYKKEDFEKVGIKGEFIQDNHSKSRYGVLRGLHFQRKPYAQAKIVRCIRGVIFDVAVDLRRNSPTFGKWVGVILSEYNKYQLYIPRGFAHGFCVLSDVAEVIYKVDNVYAPDYEEGVIWNDRDIGIEWPIDEPILSEKDKKWPTLKELVERGGVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>F6BF20</accession>
    <name>F6BF20_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Pyruvate synthase</fullName>
            <ecNumber evidence="3">1.2.7.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_1355</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.2.7.1" evidence="3"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96890.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799487.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1355"/>
    <dbReference type="EnsemblBacteria" id="AEF96890">
        <property type="protein sequence ID" value="AEF96890"/>
        <property type="gene ID" value="Metig_1355"/>
    </dbReference>
    <dbReference type="GeneID" id="10644218"/>
    <dbReference type="KEGG" id="mig:Metig_1355"/>
    <dbReference type="HOGENOM" id="CLU_002569_5_0_2"/>
    <dbReference type="OMA" id="WNDQQDS"/>
    <dbReference type="OrthoDB" id="29908at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019164">
        <property type="term" value="F:pyruvate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd07034">
        <property type="entry name" value="TPP_PYR_PFOR_IOR-alpha_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033412">
        <property type="entry name" value="PFOR_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002880">
        <property type="entry name" value="Pyrv_Fd/Flavodoxin_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009014">
        <property type="entry name" value="Transketo_C/PFOR_II"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17147">
        <property type="entry name" value="PFOR_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01855">
        <property type="entry name" value="POR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="SSF52518"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52922">
        <property type="entry name" value="SSF52922"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="POR_N" evidence="1">
        <location>
            <begin position="15"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="domain" description="PFOR_II" evidence="2">
        <location>
            <begin position="264"/>
            <end position="364"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF17147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF96890.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="386" mass="42448" checksum="2F0F60D249AE2424" modified="2011-07-27" version="1">MCKVKVITGTSAAAEAAKLADVDVIAAYPITPQTTCVEKLAEFVANGELDAEFIKVESEHSAMSACIGASATGARTFTATASQGLALMHEMLFIAAGMRLPIVMMNANRALSAPINIWNDQQDSISQRDTGWIQFYVEDNQETLDTILQAYRIGEHEDVLLPVMVNLDGFILTHTVEPVTIPSEENAKAFVGVYEPKHAYLDPDRPITQGCVGVPECYMETRYAVEKAMENAKKVIKEIHDEFAEKFKRSYGNGLIEEYNMENADTVIVAMGSVCGTIKEVIDRLKEKGKEVGLLRVRCYRPFPAEDIAKALKDAENIAVLDKNISLGINKGALYLDLASTVRDKKVVNYIVGLGGRDIRIEDIEKIISHVEVAKDGETAWIGLKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>F6BF21</accession>
    <name>F6BF21_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Pyruvate synthase</fullName>
            <ecNumber evidence="2">1.2.7.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1356</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.2.7.1" evidence="2"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96891.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1356"/>
    <dbReference type="EnsemblBacteria" id="AEF96891">
        <property type="protein sequence ID" value="AEF96891"/>
        <property type="gene ID" value="Metig_1356"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_1356"/>
    <dbReference type="HOGENOM" id="CLU_058423_0_0_2"/>
    <dbReference type="OMA" id="ATGCMEV"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019164">
        <property type="term" value="F:pyruvate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011766">
        <property type="entry name" value="TPP_enzyme-bd_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02775">
        <property type="entry name" value="TPP_enzyme_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="SSF52518"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TPP_enzyme_C" evidence="1">
        <location>
            <begin position="57"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96891.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="309" mass="34448" checksum="CED594B352F1F8C7" modified="2011-07-27" version="1">MKFLKNLKNINYGEIMQFPREEYFAPGHRGCAGCGAAIVARLVLKAAGKDTICTNATGCLEVMTTPYPETAWRVPWIHTAFENSAAVASGVESAIKALRRKRGKFNKKINVIAFGGDGGTADIGFQALSGAMERGHDIVYIMYDNEAYMNTGVQRSGSTPLMAATTTSPAGSKIRGEDRPKKDMPLIMAAHGIPYVATACISYPEDFMNKVKKACEIEGPAYIQVLQPCTTGWGYPAEKTIEIGRLAVETGIWPLYEIENGEFRLTYKPAKRKPIKEYIRMQKRYRHLTDEDIERLQKYIDERCRELGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>F6BF33</accession>
    <name>F6BF33_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Acetyl-CoA carboxylase, biotin carboxylase</fullName>
            <ecNumber evidence="7">6.4.1.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Metig_1368</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EC" id="6.4.1.1" evidence="7"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96903.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799500.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1368"/>
    <dbReference type="EnsemblBacteria" id="AEF96903">
        <property type="protein sequence ID" value="AEF96903"/>
        <property type="gene ID" value="Metig_1368"/>
    </dbReference>
    <dbReference type="GeneID" id="10644232"/>
    <dbReference type="KEGG" id="mig:Metig_1368"/>
    <dbReference type="HOGENOM" id="CLU_000395_3_2_2"/>
    <dbReference type="OMA" id="FVEICSH"/>
    <dbReference type="OrthoDB" id="36803at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004736">
        <property type="term" value="F:pyruvate carboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004549">
        <property type="entry name" value="Acetyl_CoA_COase_biotin_COase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005481">
        <property type="entry name" value="BC-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011764">
        <property type="entry name" value="Biotin_carboxylation_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005482">
        <property type="entry name" value="Biotin_COase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005479">
        <property type="entry name" value="CbamoylP_synth_lsu-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011054">
        <property type="entry name" value="Rudment_hybrid_motif"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02785">
        <property type="entry name" value="Biotin_carb_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00289">
        <property type="entry name" value="Biotin_carb_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02786">
        <property type="entry name" value="CPSase_L_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00878">
        <property type="entry name" value="Biotin_carb_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51246">
        <property type="entry name" value="SSF51246"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="SSF52440"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00514">
        <property type="entry name" value="accC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50979">
        <property type="entry name" value="BC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00866">
        <property type="entry name" value="CPSASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00867">
        <property type="entry name" value="CPSASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 7" id="KW-0436">Ligase</keyword>
    <keyword evidence="2 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Biotin carboxylation" evidence="6">
        <location>
            <begin position="1"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="domain" description="ATP-grasp" evidence="5">
        <location>
            <begin position="120"/>
            <end position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEF96903.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="504" mass="56115" checksum="3A8CCAE5E064FC4A" modified="2011-07-27" version="1">MFEKVLIANRGEIAVRIIRACKELGIKTVAIYSEADEHALYTSLADECYCIGPPPALKSYLNINAILNVAEKAGVDAIHPGYGFLSENANFARECIKRGFEFIGPSPEAIEAMGSKINAKKIMKKAGVPVLPGREEPIETEEDAVEVAEEIGYPVIIKASAGGGGMGMSVAYNREELIEKIESTKSIAKSAFGDATIFIEKYLEKPRHIEIQILGDKYGNMIHLGDRECSIQRRHQKLIEEAPSPIMTEELRERMGKAAIKAGKAINYYSAGTVEFLYSDGEFYFLEMNTRIQVEHPITEMITGIDIVKEQIKIAAGEELSIKQEDVVFRGHAIECRINAEDAINDFVPCPGKIRAYRSPGGPGIRLDSGVYAGAEIPPYYDSLIAKLVAYGRDRNEAIARMRRALSEYIIIGVTTNIPFHRAVMEEEDFIKGNLSTHYVEEHWDVLKDEMLKYAIEAKDMDKLFIEKVFQDNKKVAAIVGGLNAYISRIITNNNKEKDKYGGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-06-02" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>F6BF70</accession>
    <name>F6BF70_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_1405</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96940.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799536.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96940">
        <property type="protein sequence ID" value="AEF96940"/>
        <property type="gene ID" value="Metig_1405"/>
    </dbReference>
    <dbReference type="GeneID" id="10644278"/>
    <dbReference type="KEGG" id="mig:Metig_1405"/>
    <dbReference type="HOGENOM" id="CLU_644987_0_0_2"/>
    <dbReference type="OrthoDB" id="104965at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF96940.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="425" mass="50352" checksum="737A3FCD3A84239E" modified="2011-07-27" version="1">MKKKSNDFIGSEHIYKLQSKHTKLVNLLLNLTWPNKKFPNPLYKLGYEPKKGYCIDVELQLDEKRMANPDIILINEKKGHILFFECKSNTVKEEQANRYQFLISNLNDIINLNIREFPLKEKPGEFVGDVSYISFDDLTNNPILKKFNEFQILKVDAKEQNGKIEKIKRIVLKRGKYKVNELNDIFPIEIDDKHKPSFIMYPIDIKNGHEYIILEMYILNALITLATAGHEIPEEGMIFSEDIILKKGNYKINADIADIEEIKNFFEEHRDKIKNVESSDSIEILEDIVICKKGEPLEKWHIKVIDVFNVCIDMNKLEKRGFTPGKLLDIFRHRFGLISLNYMYRKDKNILLKKIEEILKSIISTDKFEKLLEYEKRKKKSIWKIRVMKNSRQIEAFRYKCFEIIQTRIEKAYTKQMTLDKFLNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>F6BF74</accession>
    <name>F6BF74_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="7">ABC-type transporter, integral membrane subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Metig_1409</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96944.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799540.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1409"/>
    <dbReference type="EnsemblBacteria" id="AEF96944">
        <property type="protein sequence ID" value="AEF96944"/>
        <property type="gene ID" value="Metig_1409"/>
    </dbReference>
    <dbReference type="GeneID" id="10644282"/>
    <dbReference type="KEGG" id="mig:Metig_1409"/>
    <dbReference type="HOGENOM" id="CLU_036879_0_2_2"/>
    <dbReference type="OMA" id="WPGMGTF"/>
    <dbReference type="OrthoDB" id="61149at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045621">
        <property type="entry name" value="BPD_transp_1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19300">
        <property type="entry name" value="BPD_transp_1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="9"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="105"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="138"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="173"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="235"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="280"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="6">
        <location>
            <begin position="99"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEF96944.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="311" mass="35112" checksum="9EC74B4027D4F04B" modified="2011-07-27" version="1">MIRYLSKRILHAIITILGISLISFSLLYIFPGDPVELVIRVNTGMEPTPEMIEKYKHEFGLDKPIYIQYINWLSHAIRGDFGTSWSTGEPVMKVILDRLPASMQLFFPTFILSITFAIVLGILSAIYKDRFVDNFCRIISIIGISIPHFWSGLILIWIFAVCLKILPAFGYGTLKHMILPVLTWTLSFMAIKTRFVRSTLLEVMGEDYITTARAKGLSDKDVVIKHAFRNALIPIITYFSLSISHLIIGAVVIEALFAWPGLGSLLVKSVFERDFPVVQALVFISGVLIVTVNLVVDILYAIIDPRIRYEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>F6BF77</accession>
    <name>F6BF77_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Nickel-transporting ATPase</fullName>
            <ecNumber evidence="7">3.6.3.24</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Metig_1412</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.6.3.24" evidence="7"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96947.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799543.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1412"/>
    <dbReference type="EnsemblBacteria" id="AEF96947">
        <property type="protein sequence ID" value="AEF96947"/>
        <property type="gene ID" value="Metig_1412"/>
    </dbReference>
    <dbReference type="GeneID" id="10644285"/>
    <dbReference type="KEGG" id="mig:Metig_1412"/>
    <dbReference type="HOGENOM" id="CLU_000604_1_23_2"/>
    <dbReference type="OMA" id="LGMSVVW"/>
    <dbReference type="OrthoDB" id="61385at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015833">
        <property type="term" value="P:peptide transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013563">
        <property type="entry name" value="Oligopep_ABC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08352">
        <property type="entry name" value="oligo_HPY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01727">
        <property type="entry name" value="oligo_HPY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="6">
        <location>
            <begin position="2"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="246"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="41"/>
            <end position="48"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEF96947.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="311" mass="35881" checksum="61C8DB458B717BE3" modified="2011-07-27" version="1">MIKGKNLTKVFESGIFSKRKFIAVDNVDIEIKEGETLCLIGESGSGKSTLGKMLLNLIKPTNGEVIFNNYNITKMKQTELRKIRRYMQLIPQYPDSALNPRWTIKNSILEPLRIHKLIDKNNEQEKLEELIETVGLKKEHLDRYPHELSGGELQRAVIARAISLKPKFIVCDEPTSMLDVSVQASIINLLLNLQKKYNISYLFITHDLEIANIVGQRFAVMYGGQILEEGNKIMDKPLHPYTELLVKSTTMDIENINELLDKTETEIKKSNNNRGCKYYHLCPYHDEKCLKENPPVVEVDKNRKVRCFRYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-10-19" modified="2022-01-19" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>G1UBJ0</accession>
    <name>G1UBJ0_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">50S ribosomal protein L12</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">rpl12</name>
        <name evidence="6" type="ordered locus">Metig_0943</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Forms part of the ribosomal stalk, playing a central role in the interaction of the ribosome with GTP-bound translation factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Part of the 50S ribosomal subunit. Homodimer, it forms part of the ribosomal stalk which helps the ribosome interact with GTP-bound translation factors. Forms a heptameric L10(L12)2(L12)2(L12)2 complex, where L10 forms an elongated spine to which the L12 dimers bind in a sequential fashion.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein P1/P2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96486.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799088.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0943"/>
    <dbReference type="EnsemblBacteria" id="AEF96486">
        <property type="protein sequence ID" value="AEF96486"/>
        <property type="gene ID" value="Metig_0943"/>
    </dbReference>
    <dbReference type="GeneID" id="10643789"/>
    <dbReference type="KEGG" id="mig:Metig_0943"/>
    <dbReference type="HOGENOM" id="CLU_114656_2_0_2"/>
    <dbReference type="OMA" id="EYIYAAM"/>
    <dbReference type="OrthoDB" id="128352at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006414">
        <property type="term" value="P:translational elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05832">
        <property type="entry name" value="Ribosomal_L12p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1410">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01478">
        <property type="entry name" value="Ribosomal_L12_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038716">
        <property type="entry name" value="P1/P2_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027534">
        <property type="entry name" value="Ribosomal_L12/P1/P2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022295">
        <property type="entry name" value="Ribosomal_L12_arc"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03685">
        <property type="entry name" value="ribo_P1_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 6" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="69"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="73"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEF96486.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="102" mass="10290" checksum="75FFD5C47A723340" modified="2011-10-19" version="1">MEYVYAALLLHAAGKEITEDAVKAVLSAAGVEVDEARVKALVAALEGVNIDEAIENAAMPVAAAAPAAAAAPAAEEKKEEEKKEEKKDDTAAAAAGLAALFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>F6BA70</accession>
    <name>F6BA70_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 11">Mevalonate kinase</fullName>
            <shortName evidence="11">MK</shortName>
            <shortName evidence="11">MVK</shortName>
            <ecNumber evidence="2 11">2.7.1.36</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">mvk</name>
        <name evidence="14" type="ordered locus">Metig_0203</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes the phosphorylation of (R)-mevalonate (MVA) to (R)-mevalonate 5-phosphate (MVAP). Functions in the mevalonate (MVA) pathway leading to isopentenyl diphosphate (IPP), a key precursor for the biosynthesis of isoprenoid compounds such as archaeal membrane lipids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>(R)-mevalonate + ATP = (R)-5-phosphomevalonate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17065"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:36464"/>
            <dbReference type="ChEBI" id="CHEBI:58146"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.36"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="10 11">Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via mevalonate pathway; isopentenyl diphosphate from (R)-mevalonate: step 1/3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 11">Belongs to the GHMP kinase family. Mevalonate kinase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.36" evidence="2 11"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95760.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798369.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0203"/>
    <dbReference type="EnsemblBacteria" id="AEF95760">
        <property type="protein sequence ID" value="AEF95760"/>
        <property type="gene ID" value="Metig_0203"/>
    </dbReference>
    <dbReference type="GeneID" id="10643038"/>
    <dbReference type="KEGG" id="mig:Metig_0203"/>
    <dbReference type="HOGENOM" id="CLU_017814_0_0_2"/>
    <dbReference type="OMA" id="NTVCTYG"/>
    <dbReference type="OrthoDB" id="90882at2157"/>
    <dbReference type="UniPathway" id="UPA00057">
        <property type="reaction ID" value="UER00098"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004496">
        <property type="term" value="F:mevalonate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019287">
        <property type="term" value="P:isopentenyl diphosphate biosynthetic process, mevalonate pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.890">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00217">
        <property type="entry name" value="Mevalonate_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013750">
        <property type="entry name" value="GHMP_kinase_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036554">
        <property type="entry name" value="GHMP_kinase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006204">
        <property type="entry name" value="GHMP_kinase_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006203">
        <property type="entry name" value="GHMP_knse_ATP-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006205">
        <property type="entry name" value="Mev_gal_kin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022937">
        <property type="entry name" value="Mevalonate_kinase_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43290">
        <property type="entry name" value="PTHR43290"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08544">
        <property type="entry name" value="GHMP_kinases_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00288">
        <property type="entry name" value="GHMP_kinases_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55060">
        <property type="entry name" value="SSF55060"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00549">
        <property type="entry name" value="mevalon_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00627">
        <property type="entry name" value="GHMP_KINASES_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="7 11 14" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 11" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="9 11" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11 14" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GHMP_kinases_N" evidence="12">
        <location>
            <begin position="101"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="domain" description="GHMP_kinases_C" evidence="13">
        <location>
            <begin position="238"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="11">
        <location>
            <position position="160"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="109"/>
            <end position="119"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00029438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF08544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="AEF95760.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="317" mass="35530" checksum="1DE3D47ECE2DF11D" modified="2011-07-27" version="1">MVVVESPAKVILFGEHAVVYGYRAISMAIDLKTTCTVEESDTTIINLKDLKKSLKIPIEEILNLNVNEYGDFRYVLCAIKNTILYLLSHKNLDLKDLKPFKLTITSQIPISCGLGSSASTTISTIKGISAFYERPLDDNEIATIGYHVEKEVQGKASVTDTATITYKGLLEIENNTIRRIEGGLKDFIKSCKFLICYCEERKKKTAELVNEVANHKNRDDIFKLIEKVVDTSKTTFDKEIFGELMYINHNLLKRLGVSTPKLDEVVEIGKTYGYGAKLTGAGGGGCVIILVNENKEDELINKLKEKGVEYFECRMVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>F6BA85</accession>
    <name>F6BA85_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_0218</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95775.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798384.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF95775">
        <property type="protein sequence ID" value="AEF95775"/>
        <property type="gene ID" value="Metig_0218"/>
    </dbReference>
    <dbReference type="GeneID" id="10643053"/>
    <dbReference type="KEGG" id="mig:Metig_0218"/>
    <dbReference type="HOGENOM" id="CLU_2140308_0_0_2"/>
    <dbReference type="OMA" id="GHFFLEQ"/>
    <dbReference type="OrthoDB" id="104308at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF95775.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="115" mass="13905" checksum="C86F33E5E7649232" modified="2011-07-27" version="1">MKTIETKDIKLKKVITKTDAELYVIELSKNHFFIEQNPLKKSKYGEAYRKLKEKYPEFYMFWEIKNNRYTGKLLAGLIVKKKDIDGFITDIIKSDDYKKFEDIMDEIEEYEQEED</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-06-02" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>F6BA89</accession>
    <name>F6BA89_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0222</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95779.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798388.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF95779">
        <property type="protein sequence ID" value="AEF95779"/>
        <property type="gene ID" value="Metig_0222"/>
    </dbReference>
    <dbReference type="GeneID" id="10643057"/>
    <dbReference type="KEGG" id="mig:Metig_0222"/>
    <dbReference type="HOGENOM" id="CLU_198706_0_0_2"/>
    <dbReference type="OMA" id="EAIPFSH"/>
    <dbReference type="OrthoDB" id="118484at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="30"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="56"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF95779.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="80" mass="8980" checksum="473F01935779A1F0" modified="2011-07-27" version="1">MDIGLYFAPIVGFLIGMVLGTKFREKIKVISFYLIVGAIIAYFLKPLPYYNFPVPLSLTYFLSVLGIIVGNLLFGGKWVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAB0</accession>
    <name>F6BAB0_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glycosyl transferase group 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0243</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95800.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798409.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0243"/>
    <dbReference type="EnsemblBacteria" id="AEF95800">
        <property type="protein sequence ID" value="AEF95800"/>
        <property type="gene ID" value="Metig_0243"/>
    </dbReference>
    <dbReference type="GeneID" id="10643078"/>
    <dbReference type="KEGG" id="mig:Metig_0243"/>
    <dbReference type="HOGENOM" id="CLU_009583_2_2_2"/>
    <dbReference type="OMA" id="FCEHIVI"/>
    <dbReference type="OrthoDB" id="83551at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glycos_transf_1" evidence="1">
        <location>
            <begin position="200"/>
            <end position="369"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF95800.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="391" mass="46279" checksum="EE014004E8133608" modified="2011-07-27" version="1">MKVNHIKNKKYVNENIKIYYIENFSIPGNSAHSLQIENTIKALSKISNVTLISISFNTVKKLDFCEHIVIKSPDLFKKIIPYRVFYLLYKIKDLKFEKNAYLYTRSPIIAYYLHKKFKKVIVEIHNIPNLKDNIFENLLFHIPLNNFFLNKLYNRENIHFVTISKSLKEDLIKLFSIPEEKITVLPDAVDLNKFNINISKEDAREKLNLPKEKIIITYTGSLQEWKGYKTFLESYKYLKNRKNVIYLIVGGSEDQIKILKKEYENENIIFIPFVEHSKIPLFLKVSDILVIPNSAKYEISVKYTSPLKLFEYMASRRPIIASDLPSIREIVSEEEVLFFRPDNEKDLAEKIEYLLNNKDLIEKLAKNAFEKVKNYTWEKRAKRIVEIFEVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAF3</accession>
    <name>F6BAF3_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Saccharop_dh_N domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1431</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96966.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1431"/>
    <dbReference type="EnsemblBacteria" id="AEF96966">
        <property type="protein sequence ID" value="AEF96966"/>
        <property type="gene ID" value="Metig_1431"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_1431"/>
    <dbReference type="HOGENOM" id="CLU_056125_0_0_2"/>
    <dbReference type="OMA" id="GHKHHIY"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd12144">
        <property type="entry name" value="SDH_N_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005239">
        <property type="entry name" value="CHP00300"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007545">
        <property type="entry name" value="LOR/SDH_bifunc_enz_cons_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04455">
        <property type="entry name" value="Saccharop_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00300">
        <property type="entry name" value="TIGR00300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Saccharop_dh_N" evidence="1">
        <location>
            <begin position="2"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96966.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="420" mass="46885" checksum="54DA4B7E150A6AA3" modified="2011-07-27" version="1">MREIELRGHIIDSLILPKVFDKILDLGGDYKVLEFEIGKRKTDPSYAKILVIGRDEEHVDEILMELRDIGAEIPEIEEVELKEAERDMVLPDGFYSTTNHPTFIRYKGEWIEVENPKMDGVIVVYPEEKRAEVKTIRKVKKGDLIVVGHKGIRVIPPEKPREKGELFEFMKSEASSEKPKETIIRKIAKEMYAIREEYRKTGKGGIVVVGGPAIVHTGAGEALAKLIRMGYVQALFSGNALATHDIEWALYGTSLGVNIETGKPVSGGHRHHLYAINTIMKAGSIKAAVEKGILKKGIMYECIKNNVPYVLAGSIRDDGPLPDVITDVVEAQDKMREMLKGKKMVLMLSTMLHSIATGNLLPSYVKTICVDINPATVTKLMDRGTSQAIGVVTDVGVFLPMLVEELERLEKENKKKNNEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAG3</accession>
    <name>F6BAG3_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">RNA-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_1441</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96976.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96976">
        <property type="protein sequence ID" value="AEF96976"/>
        <property type="gene ID" value="Metig_1441"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_1441"/>
    <dbReference type="HOGENOM" id="CLU_200895_3_0_2"/>
    <dbReference type="OMA" id="CSCNLYF"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019300">
        <property type="entry name" value="CooT"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10133">
        <property type="entry name" value="CooT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF96976.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="56" mass="6472" checksum="7AD7248E9B06BBA1" modified="2011-07-27" version="1">MCSCNLYLNDELVMEDVILVEKKDNKIIAVDLFGERKEFEGEIKKIDLNNNKIIIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAH7</accession>
    <name>F6BAH7_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="1 3">Nucleoside diphosphate kinase</fullName>
            <shortName evidence="1">NDK</shortName>
            <shortName evidence="1">NDP kinase</shortName>
            <ecNumber evidence="1 3">2.7.4.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Nucleoside-2-P kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ndk</name>
        <name evidence="5" type="ordered locus">Metig_1455</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 3">
            <text>a 2'-deoxyribonucleoside 5'-diphosphate + ATP = a 2'-deoxyribonucleoside 5'-triphosphate + ADP</text>
            <dbReference type="Rhea" id="RHEA:44640"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:73316"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.6"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a ribonucleoside 5'-diphosphate + ATP = a ribonucleoside 5'-triphosphate + ADP</text>
            <dbReference type="Rhea" id="RHEA:18113"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57930"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the NDK family.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.6" evidence="1 3"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96990.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799586.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1455"/>
    <dbReference type="EnsemblBacteria" id="AEF96990">
        <property type="protein sequence ID" value="AEF96990"/>
        <property type="gene ID" value="Metig_1455"/>
    </dbReference>
    <dbReference type="GeneID" id="10644328"/>
    <dbReference type="KEGG" id="mig:Metig_1455"/>
    <dbReference type="HOGENOM" id="CLU_060216_6_3_2"/>
    <dbReference type="OMA" id="KIVAMKM"/>
    <dbReference type="OrthoDB" id="106462at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004550">
        <property type="term" value="F:nucleoside diphosphate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006241">
        <property type="term" value="P:CTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006183">
        <property type="term" value="P:GTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006165">
        <property type="term" value="P:nucleoside diphosphate phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006228">
        <property type="term" value="P:UTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.141">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00451">
        <property type="entry name" value="NDP_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034907">
        <property type="entry name" value="NDK-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036850">
        <property type="entry name" value="NDK-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001564">
        <property type="entry name" value="Nucleoside_diP_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023005">
        <property type="entry name" value="Nucleoside_diP_kinase_AS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00334">
        <property type="entry name" value="NDK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01243">
        <property type="entry name" value="NUCDPKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00562">
        <property type="entry name" value="NDK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54919">
        <property type="entry name" value="SSF54919"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00469">
        <property type="entry name" value="NDP_KINASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1 3 5" id="KW-0418">Kinase</keyword>
    <keyword evidence="1" id="KW-0460">Magnesium</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0546">Nucleotide metabolism</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="NDK" evidence="4">
        <location>
            <begin position="2"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="active site" description="Pros-phosphohistidine intermediate" evidence="1">
        <location>
            <position position="116"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="10"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="58"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="86"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="92"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="103"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="113"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU004011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU004013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEF96990.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="136" mass="15673" checksum="D60B4688011C80E2" modified="2011-07-27" version="1">MKERTFVLLKPDAVRRKLIGRIIQRFEDKGFEIVEMKMITLSKEMAEEFYKMHKGKDFYERLIEFMTSGRVVAMIIEGENAISVVRKMIGKTNPAEAEPGTIRGDFALSTPDNLIHASDSKENAEREIKLFFGKLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAI3</accession>
    <name>F6BAI3_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Major facilitator superfamily MFS_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_1461</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96996.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799592.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1461"/>
    <dbReference type="EnsemblBacteria" id="AEF96996">
        <property type="protein sequence ID" value="AEF96996"/>
        <property type="gene ID" value="Metig_1461"/>
    </dbReference>
    <dbReference type="GeneID" id="10644334"/>
    <dbReference type="KEGG" id="mig:Metig_1461"/>
    <dbReference type="HOGENOM" id="CLU_001265_10_14_2"/>
    <dbReference type="OMA" id="LYADSFH"/>
    <dbReference type="OrthoDB" id="22281at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001958">
        <property type="entry name" value="Tet-R_TetA/multi-R_MdtG"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01035">
        <property type="entry name" value="TCRTETA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="9"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="45"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="72"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="97"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="133"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="159"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="209"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="237"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="269"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="294"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="332"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="359"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="2">
        <location>
            <begin position="7"/>
            <end position="385"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF96996.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="385" mass="41890" checksum="F27EE18F5D9E5DF2" modified="2011-07-27" version="1">MEKPVRSMFVIWVTTFVTMLGVGFIAPIMSIYAQTLGATNFEIGLIFGSFALARTIAQIPAGVLSDIYGKKFFIVSGAFFYGVFTLLYNFVNTVLGLLIVRTFTGIFSSFVTPVAGSYVAAIAPKTRLGEYMGIFNSAITLGFGVGPFVGGILADMYGIKTPFYFCGFLGILAAIISYTKLEDIAFKNIGNTKNKIKLFSFEFLRNRNFLSSFVINTSNVMVNAGIITYLALYATNYGITMGRIGFMIAATNLLMALLQRNFGKLYDRLGNVVIIFGIFIMSFGMYMLSISSSFLSMVASLTIVAIGSSICTPATTSLAIKDIPTHRKGEAMGLFTTSINIGMFIGSIFFGFLADILGIANMFKVSAIFSSIIGFISYLRIKNNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAJ0</accession>
    <name>F6BAJ0_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Phosphoadenosine phosphosulfate reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1469</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97003.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799599.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1469"/>
    <dbReference type="EnsemblBacteria" id="AEF97003">
        <property type="protein sequence ID" value="AEF97003"/>
        <property type="gene ID" value="Metig_1469"/>
    </dbReference>
    <dbReference type="GeneID" id="10644342"/>
    <dbReference type="KEGG" id="mig:Metig_1469"/>
    <dbReference type="HOGENOM" id="CLU_026622_0_0_2"/>
    <dbReference type="OMA" id="FERIGCY"/>
    <dbReference type="OrthoDB" id="31010at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01713">
        <property type="entry name" value="PAPS_reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002500">
        <property type="entry name" value="PAPS_reduct"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01507">
        <property type="entry name" value="PAPS_reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PAPS_reduct" evidence="1">
        <location>
            <begin position="186"/>
            <end position="357"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF97003.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="416" mass="49698" checksum="8B2525432BBF6845" modified="2011-07-27" version="1">MFKEDTKFASKFEIDVLNELTNGNFAYDDLILLEKMSGLDYRKRVYLNNTQIGTLEFDLVDLKWKFIPSAGYYLIEEPKIKIKPTKRKLKGKWVDESLIENIEELNQIDDEEYVGVSVGKFVGVAVKRKGKLKVKDLTFKREIETKKIQDFLKENEDRIRKMEKKSIEFIRRYVEKYKNKSNYVINASFSGGKDSSISTLLATKVVEDIDVLFIDTGLEYPDTIDFVKKFAKEYDLNLHVIKAGDFWKELEKEGIPTKDNRWCNSVCKLIPLKEFFEEEYKNKKIITIDGTRKYESFTRAKLKYERRNRFIPFQTSIFPILDWNALDVWSYIYINNILYNPLYDKGFERIGCYLCPAALNSEFLRVRDLYPKLFNRWFNYLKKFYAEEDALRGFWRWKEFPPKMKELRKILEEKNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAJ1</accession>
    <name>F6BAJ1_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">DNA methylase N-4/N-6 domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_1470</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97004.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799600.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1470"/>
    <dbReference type="EnsemblBacteria" id="AEF97004">
        <property type="protein sequence ID" value="AEF97004"/>
        <property type="gene ID" value="Metig_1470"/>
    </dbReference>
    <dbReference type="GeneID" id="10644343"/>
    <dbReference type="KEGG" id="mig:Metig_1470"/>
    <dbReference type="HOGENOM" id="CLU_069059_0_0_2"/>
    <dbReference type="OMA" id="VHIEFPR"/>
    <dbReference type="OrthoDB" id="60331at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF97004.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="285" mass="32489" checksum="603669D8C6A8FF9F" modified="2011-07-27" version="1">MLSKETIIKKIKSYKPCKKCNKPLPKTVPIEKLNLKNRKNICECGKRHIDDVMLNVYEIMNNFGEFKSENVSLKDVGVPLIEVGYPLKYLPVLRENELIIMADVSRECAEEIVKIKEIKGVLSTNQKFSSNSSDINKLLAGDDFRCDVFPLGNDCIIVCKNQSKVHIEFPRPYNPKVSKIKRLNLKNKVVIDGFCGVGTLGMVALKMGAKKVIFCDINKHAIDNLIYNMELNFGEEIFERVEIFNADFLDLDVKGDICFVDLFPYMEPDIYLKKAKEIADEVVII</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAJ4</accession>
    <name>F6BAJ4_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Glutamine--fructose-6-phosphate transaminase (isomerizing)</fullName>
            <ecNumber evidence="2">2.6.1.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">Metig_1473</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>D-fructose 6-phosphate + L-glutamine = D-glucosamine 6-phosphate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:13237"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:58725"/>
            <dbReference type="ChEBI" id="CHEBI:61527"/>
            <dbReference type="EC" id="2.6.1.16"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.6.1.16" evidence="2"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97007.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1473"/>
    <dbReference type="EnsemblBacteria" id="AEF97007">
        <property type="protein sequence ID" value="AEF97007"/>
        <property type="gene ID" value="Metig_1473"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_1473"/>
    <dbReference type="HOGENOM" id="CLU_285704_0_0_2"/>
    <dbReference type="OMA" id="NSRWATH"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097367">
        <property type="term" value="F:carbohydrate derivative binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004360">
        <property type="term" value="F:glutamine-fructose-6-phosphate transaminase (isomerizing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901137">
        <property type="term" value="P:carbohydrate derivative biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006541">
        <property type="term" value="P:glutamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016539">
        <property type="term" value="P:intein-mediated protein splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05008">
        <property type="entry name" value="SIS_GlmS_GlmD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05009">
        <property type="entry name" value="SIS_GlmS_GlmD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.28.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017932">
        <property type="entry name" value="GATase_2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035466">
        <property type="entry name" value="GlmS/AgaS_SIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035490">
        <property type="entry name" value="GlmS/FrlB_SIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005855">
        <property type="entry name" value="GlmS_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003586">
        <property type="entry name" value="Hint_dom_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003587">
        <property type="entry name" value="Hint_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036844">
        <property type="entry name" value="Hint_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027434">
        <property type="entry name" value="Homing_endonucl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006142">
        <property type="entry name" value="INTEIN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030934">
        <property type="entry name" value="Intein_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004042">
        <property type="entry name" value="Intein_endonuc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006141">
        <property type="entry name" value="Intein_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004860">
        <property type="entry name" value="LAGLIDADG_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001347">
        <property type="entry name" value="SIS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046348">
        <property type="entry name" value="SIS_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10937:SF0">
        <property type="entry name" value="PTHR10937:SF0"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13537">
        <property type="entry name" value="GATase_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12844">
        <property type="entry name" value="HTH_19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14528">
        <property type="entry name" value="LAGLIDADG_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01380">
        <property type="entry name" value="SIS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00379">
        <property type="entry name" value="INTEIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00305">
        <property type="entry name" value="HintC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00306">
        <property type="entry name" value="HintN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00530">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51294">
        <property type="entry name" value="SSF51294"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53697">
        <property type="entry name" value="SSF53697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55608">
        <property type="entry name" value="SSF55608"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="SSF56235"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01135">
        <property type="entry name" value="glmS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01443">
        <property type="entry name" value="intein_Cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01445">
        <property type="entry name" value="intein_Nterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51278">
        <property type="entry name" value="GATASE_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50818">
        <property type="entry name" value="INTEIN_C_TER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50819">
        <property type="entry name" value="INTEIN_ENDONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50817">
        <property type="entry name" value="INTEIN_N_TER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51464">
        <property type="entry name" value="SIS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="13" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="5" id="KW-0068">Autocatalytic cleavage</keyword>
    <keyword evidence="6" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="7" id="KW-0651">Protein splicing</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 13" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glutamine amidotransferase type-2" evidence="11">
        <location>
            <begin position="2"/>
            <end position="723"/>
        </location>
    </feature>
    <feature type="domain" description="HTH cro/C1-type" evidence="10">
        <location>
            <begin position="198"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="domain" description="DOD-type homing endonuclease" evidence="9">
        <location>
            <begin position="278"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="domain" description="INTEIN_C_TER" evidence="8">
        <location>
            <begin position="549"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="domain" description="SIS" evidence="12">
        <location>
            <begin position="785"/>
            <end position="922"/>
        </location>
    </feature>
    <feature type="domain" description="SIS" evidence="12">
        <location>
            <begin position="947"/>
            <end position="1088"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50818"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AEF97007.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="1098" mass="125384" checksum="9EE32759F501A79B" modified="2011-07-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAK1</accession>
    <name>F6BAK1_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1480</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97014.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799609.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1480"/>
    <dbReference type="EnsemblBacteria" id="AEF97014">
        <property type="protein sequence ID" value="AEF97014"/>
        <property type="gene ID" value="Metig_1480"/>
    </dbReference>
    <dbReference type="GeneID" id="10644353"/>
    <dbReference type="KEGG" id="mig:Metig_1480"/>
    <dbReference type="HOGENOM" id="CLU_067083_0_0_2"/>
    <dbReference type="OMA" id="AVMGPVD"/>
    <dbReference type="OrthoDB" id="78392at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002825">
        <property type="entry name" value="Pept_S49_ser-pept_pro"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01972">
        <property type="entry name" value="SDH_sah"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="SSF52096"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF97014.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="273" mass="31491" checksum="197560EDAC13931B" modified="2011-07-27" version="1">MDPLSSLFWLFFIFMLIYPQMMFKFRLLQRYKCIKELEAERKTRVIVLIHRQEQLALFGIPIYRFISIEDSEEVLRAIRMTPDDMPIDIILHTPGGLVLAAEQIAMALKEHKAKTTAIIPHYAMSGGTLIALAADEIIMDKNAVMGPVDPQIGQYPAASILKTLEMKHADELDDETLILADISKKAINQVEEFVFELLKDKMDEEKARKLAKTLSHGEWTHDYPLTVKKLRELGIEVNTNVPKKVYELFDLYQQPVNQRPSVQYIPVPYHPKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAK3</accession>
    <name>F6BAK3_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Precorrin-2 dehydrogenase</fullName>
            <ecNumber evidence="3">1.3.1.76</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Metig_1482</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>NAD(+) + precorrin-2 = 2 H(+) + NADH + sirohydrochlorin</text>
            <dbReference type="Rhea" id="RHEA:15613"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:58351"/>
            <dbReference type="ChEBI" id="CHEBI:58827"/>
            <dbReference type="EC" id="1.3.1.76"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Porphyrin-containing compound metabolism; siroheme biosynthesis; sirohydrochlorin from precorrin-2: step 1/1.</text>
    </comment>
    <dbReference type="EC" id="1.3.1.76" evidence="3"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97016.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799611.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1482"/>
    <dbReference type="EnsemblBacteria" id="AEF97016">
        <property type="protein sequence ID" value="AEF97016"/>
        <property type="gene ID" value="Metig_1482"/>
    </dbReference>
    <dbReference type="GeneID" id="10644355"/>
    <dbReference type="KEGG" id="mig:Metig_1482"/>
    <dbReference type="HOGENOM" id="CLU_011276_8_2_2"/>
    <dbReference type="OMA" id="THVVVPY"/>
    <dbReference type="OrthoDB" id="94240at2157"/>
    <dbReference type="UniPathway" id="UPA00262">
        <property type="reaction ID" value="UER00222"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004325">
        <property type="term" value="F:ferrochelatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043115">
        <property type="term" value="F:precorrin-2 dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019354">
        <property type="term" value="P:siroheme biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028161">
        <property type="entry name" value="Met8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006367">
        <property type="entry name" value="Sirohaem_synthase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35330">
        <property type="entry name" value="PTHR35330"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01470">
        <property type="entry name" value="cysG_Nterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0520">NAD</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012400"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEF97016.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="205" mass="23781" checksum="4BDBE4A80D6B0119" modified="2011-07-27" version="1">MIPVFVNLEGFRVAIFGFGEVGKRRAKKLLKANANIDIYSKTFDDEEFEKKINFIKCDVNEISDGDLEKIIKKYDIIVTAIDKKNNERIVKIARKLGKFVNSATFEDEANLIIPACTEVDGVLFAVYTKGKSPLIAREIRKLVESYLLYHEEDLIIQSYVREKLKEKISNQKERKEILEKLFKNDEFKKELSKLIGKYGKIENHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAL1</accession>
    <name>F6BAL1_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Glyco_tran_28_C domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_1490</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97024.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1490"/>
    <dbReference type="EnsemblBacteria" id="AEF97024">
        <property type="protein sequence ID" value="AEF97024"/>
        <property type="gene ID" value="Metig_1490"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_1490"/>
    <dbReference type="HOGENOM" id="CLU_060247_0_0_2"/>
    <dbReference type="OMA" id="FGHTTRC"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016758">
        <property type="term" value="F:hexosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005262">
        <property type="entry name" value="CHP00661"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007235">
        <property type="entry name" value="Glyco_trans_28_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04101">
        <property type="entry name" value="Glyco_tran_28_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00661">
        <property type="entry name" value="MJ1255"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glyco_tran_28_C" evidence="2">
        <location>
            <begin position="202"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="317"/>
            <end position="344"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF97024.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="394" mass="45714" checksum="6D53B9AE2E4A88F6" modified="2011-07-27" version="1">MVKLMKILISVCGEGFGHTTRCIAVAEELKNHYDIAFIAYGKSKNYIESLGYEVFETYPEIKLTGYEGKFDIKKSIFNKEYNPTKAIKREREIIKHYNPDLIISDCKYSTIIASKFLKKPYYIITNQNYTKTDKKEKFIVYPVMSMLNVINRSAEEVLVPDLPLPYTICEYNLKIIDNLEFIGPLIRYKPKNIKDGGYILSVIGGFEYRYRILKYLAEIAVKNDLNVKLVCGSWGVGRELESYVNKLTDNPKNIEIIPFTNNMEKLIKNCSFIVCHGGHSTIMEGISFGKPLIVIPDLNHPEQENNAKKVQDLACGITISHNEIEKLEMAINEIQNNKIYAENAKKLKKLCKQYNGRENIRKIISKRLKRKKRNLMTRIKQEYLRTIHKIRTKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAM3</accession>
    <name>F6BAM3_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Phosphate transport system permease protein PstA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Metig_0281</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cell membrane</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the binding-protein-dependent transport system permease family. CysTW subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95837.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798446.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0281"/>
    <dbReference type="EnsemblBacteria" id="AEF95837">
        <property type="protein sequence ID" value="AEF95837"/>
        <property type="gene ID" value="Metig_0281"/>
    </dbReference>
    <dbReference type="GeneID" id="10643116"/>
    <dbReference type="KEGG" id="mig:Metig_0281"/>
    <dbReference type="HOGENOM" id="CLU_033621_2_0_2"/>
    <dbReference type="OMA" id="GIARWKA"/>
    <dbReference type="OrthoDB" id="86965at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005887">
        <property type="term" value="C:integral component of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005315">
        <property type="term" value="F:inorganic phosphate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035435">
        <property type="term" value="P:phosphate ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005672">
        <property type="entry name" value="Phosphate_PstA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00974">
        <property type="entry name" value="3a0107s02c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="20"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="70"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="110"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="138"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="197"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="255"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="7">
        <location>
            <begin position="71"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEF95837.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="284" mass="30785" checksum="84A28DF634506E04" modified="2011-07-27" version="1">MSANNINLHRIIRTIKNNIFLLIVTIGTFVAILPLFHIIYTIFLKGMPIVMERNINFITGTLSEGGIGPAIVGTLMLTAMAMIIGIPLAFLAGSYVYEFPNSLIGRVTKVLLQIMLEFPTILVGTFVTALVVVPMGHYSGLAGAIALAIILTPYVAVYTEEAMAEVPRIYKEGAYALGSTRIQVIFKVITKIAKKGILTGMLIGMAKVAGETAPLLFTAGGLYETYPTNPLEPVGAVPLLIYDLIQSPSPEDHKIAWGAALVMLFIFLAIFTPIRYALKDDIKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAX1</accession>
    <name>F6BAX1_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glycosyl transferase family 9</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1524</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97058.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799652.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1524"/>
    <dbReference type="EnsemblBacteria" id="AEF97058">
        <property type="protein sequence ID" value="AEF97058"/>
        <property type="gene ID" value="Metig_1524"/>
    </dbReference>
    <dbReference type="GeneID" id="10644397"/>
    <dbReference type="KEGG" id="mig:Metig_1524"/>
    <dbReference type="HOGENOM" id="CLU_038371_0_0_2"/>
    <dbReference type="OMA" id="FAIFSPW"/>
    <dbReference type="OrthoDB" id="132865at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03789">
        <property type="entry name" value="GT9_LPS_heptosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002201">
        <property type="entry name" value="Glyco_trans_9"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01075">
        <property type="entry name" value="Glyco_transf_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF97058.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="385" mass="45517" checksum="A95F56921B29375D" modified="2011-07-27" version="1">MKRIYKNPKDLGRYMKYIKIVDFIGDCIFFYKKRKKFKKENIKKILVIRLDHIGDMILLSSFLRNLKNNFPNSEITVLCREMTKDVAECIPYIDAIQILNTPWLSRNDRDSWKKILKYIIKYYKKYDLVFDLRGNPLHNIIVSLIGKYSVGYGCKGLGFLLNKEIIWENKPKHTVDRTLDAIRALNLPVDSGKLELKIDEKYYKNLKNKLNSIDFDKDFIILCHPISGNKCKNWLFNYWNELNNLILKENENIIILYGGSKNDYYIINKNINIDNKRIFNIAGELSLKEYFALIDLCNLLISVDTFAVHARAAFNKPLIGIYSGTNYLHEWEPYTSKKIVFQDKSCPYYPCESIKCLLKEHVCMKNIRPEIVFEAYKKLRENYEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAY1</accession>
    <name>F6BAY1_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metig_1534</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97068.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1534"/>
    <dbReference type="EnsemblBacteria" id="AEF97068">
        <property type="protein sequence ID" value="AEF97068"/>
        <property type="gene ID" value="Metig_1534"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_1534"/>
    <dbReference type="HOGENOM" id="CLU_005533_4_3_2"/>
    <dbReference type="OMA" id="CKCWELQ"/>
    <dbReference type="OrthoDB" id="44342at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008171">
        <property type="term" value="F:O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031725">
        <property type="entry name" value="ASMT_dimerisation"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016461">
        <property type="entry name" value="COMT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001077">
        <property type="entry name" value="O_MeTrfase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11746">
        <property type="entry name" value="PTHR11746"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16864">
        <property type="entry name" value="Dimerisation2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00891">
        <property type="entry name" value="Methyltransf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51683">
        <property type="entry name" value="SAM_OMT_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Dimerisation2" evidence="3">
        <location>
            <begin position="29"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="domain" description="Methyltransf_2" evidence="2">
        <location>
            <begin position="156"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF16864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEF97068.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="348" mass="41014" checksum="E23BD9910D339284" modified="2011-07-27" version="1">MLKFPEENPKELLKLFEEVYSNARIFYLLKTALDLNIFDYLDEFKSAEELARGLKTDPFLTEYILKILHKLGILEFKDNKYRNRGLANIYLRRDSEFNIITPLNYYFENIKNWENLKLILENKLKNKNESLGKMFSKVIKRMADECKCWELKKVLDYVSKYDEFKNAKKLLDLAGGHGLYAIGFSMLNENLKCYVFDLPEVIEETKKFIEKYNAKNVFTIAGDFYGDDIGSGYDIIFTSYNPGGKNPKIAKKVYDSLNKGGLFINKQFFLEVEENIMDYLNNMEWNFFRPKGLKKNKLRYTFEGDLKFSEYISYLKSIGFEILDIVDMSKLLDNEYYSSTKMIVAKKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>F6BB35</accession>
    <name>F6BB35_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 5">Shikimate dehydrogenase (NADP(+))</fullName>
            <shortName evidence="5">SDH</shortName>
            <ecNumber evidence="2 5">1.1.1.25</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">aroE</name>
        <name evidence="10" type="ordered locus">Metig_0364</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Involved in the biosynthesis of the chorismate, which leads to the biosynthesis of aromatic amino acids. Catalyzes the reversible NADPH linked reduction of 3-dehydroshikimate (DHSA) to yield shikimate (SA).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>NADP(+) + shikimate = 3-dehydroshikimate + H(+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:17737"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16630"/>
            <dbReference type="ChEBI" id="CHEBI:36208"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.25"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 5">Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 4/7.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the shikimate dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.25" evidence="2 5"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95920.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798529.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0364"/>
    <dbReference type="EnsemblBacteria" id="AEF95920">
        <property type="protein sequence ID" value="AEF95920"/>
        <property type="gene ID" value="Metig_0364"/>
    </dbReference>
    <dbReference type="GeneID" id="10643201"/>
    <dbReference type="KEGG" id="mig:Metig_0364"/>
    <dbReference type="HOGENOM" id="CLU_044063_4_1_2"/>
    <dbReference type="OMA" id="FGNPIKH"/>
    <dbReference type="OrthoDB" id="98644at2157"/>
    <dbReference type="UniPathway" id="UPA00053">
        <property type="reaction ID" value="UER00087"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004764">
        <property type="term" value="F:shikimate 3-dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009073">
        <property type="term" value="P:aromatic amino acid family biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:cellular amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009423">
        <property type="term" value="P:chorismate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019632">
        <property type="term" value="P:shikimate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00222">
        <property type="entry name" value="Shikimate_DH_AroE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046346">
        <property type="entry name" value="Aminiacid_DH-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041121">
        <property type="entry name" value="SDH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011342">
        <property type="entry name" value="Shikimate_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013708">
        <property type="entry name" value="Shikimate_DH-bd_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022893">
        <property type="entry name" value="Shikimate_DH_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006151">
        <property type="entry name" value="Shikm_DH/Glu-tRNA_Rdtase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21089">
        <property type="entry name" value="PTHR21089"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18317">
        <property type="entry name" value="SDH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01488">
        <property type="entry name" value="Shikimate_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08501">
        <property type="entry name" value="Shikimate_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53223">
        <property type="entry name" value="SSF53223"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00507">
        <property type="entry name" value="aroE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="4 5" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0521">NADP</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Shikimate_dh_N" evidence="8">
        <location>
            <begin position="11"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="domain" description="Shikimate_DH" evidence="7">
        <location>
            <begin position="116"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="domain" description="SDH_C" evidence="9">
        <location>
            <begin position="247"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="region of interest" description="Shikimate binding" evidence="5">
        <location>
            <begin position="19"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="149"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="5">
        <location>
            <position position="70"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="66"/>
        </location>
        <ligand>
            <name>shikimate</name>
            <dbReference type="ChEBI" id="CHEBI:36208"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="82"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="91"/>
        </location>
        <ligand>
            <name>shikimate</name>
            <dbReference type="ChEBI" id="CHEBI:36208"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="106"/>
        </location>
        <ligand>
            <name>shikimate</name>
            <dbReference type="ChEBI" id="CHEBI:36208"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="131"/>
            <end position="135"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="153"/>
            <end position="158"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="224"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="226"/>
        </location>
        <ligand>
            <name>shikimate</name>
            <dbReference type="ChEBI" id="CHEBI:36208"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="247"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="254"/>
        </location>
        <ligand>
            <name>shikimate</name>
            <dbReference type="ChEBI" id="CHEBI:36208"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF08501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF18317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AEF95920.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="283" mass="31294" checksum="7119B0852AFDDD08" modified="2011-07-27" version="1">MINAKTKLIGLLGHPVEHSFSPIMHNAAFRDKNLNYVYLAFDVLPENLKYVIDGAKAMGIVGFNVTIPHKIEIMKYLDEIDENAKLIGAVNTIKIENNKAVGYNTDGLGARMALEEEIGKKVENKNIIIYGAGGAARAVAFELAKNNNIIIANRTVEKAKNLAEEIREKLNKTEEEVKYSGLDANLDGIDIIINATPIGMYPNVDVKPIVKAEQMHKDMVVMDLIYNPLETVLLKEAKKAGAKAINGLGMLIYQGAIAFEIWTNEKPNIEVMKNAIINHLNLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>F6BB37</accession>
    <name>F6BB37_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="5">AAA family ATPase, CDC48 subfamily</fullName>
            <ecNumber evidence="5">3.6.1.3</ecNumber>
            <ecNumber evidence="5">3.6.4.3</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Metig_0366</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AAA ATPase family. CDC48 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.3" evidence="5"/>
    <dbReference type="EC" id="3.6.4.3" evidence="5"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95922.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798531.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0366"/>
    <dbReference type="EnsemblBacteria" id="AEF95922">
        <property type="protein sequence ID" value="AEF95922"/>
        <property type="gene ID" value="Metig_0366"/>
    </dbReference>
    <dbReference type="GeneID" id="10643203"/>
    <dbReference type="KEGG" id="mig:Metig_0366"/>
    <dbReference type="HOGENOM" id="CLU_000688_12_2_2"/>
    <dbReference type="OMA" id="HACHDIK"/>
    <dbReference type="OrthoDB" id="2808at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005938">
        <property type="entry name" value="AAA_ATPase_CDC48"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009010">
        <property type="entry name" value="Asp_de-COase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004201">
        <property type="entry name" value="Cdc48_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029067">
        <property type="entry name" value="CDC48_domain_2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003338">
        <property type="entry name" value="CDC4_N-term_subdom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02933">
        <property type="entry name" value="CDC48_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02359">
        <property type="entry name" value="CDC48_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01072">
        <property type="entry name" value="CDC48_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01073">
        <property type="entry name" value="CDC48_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50692">
        <property type="entry name" value="SSF50692"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54585">
        <property type="entry name" value="SSF54585"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01243">
        <property type="entry name" value="CDC48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CDC48_N" evidence="4">
        <location>
            <begin position="5"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="domain" description="CDC48_2" evidence="3">
        <location>
            <begin position="104"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="domain" description="AAA" evidence="2">
        <location>
            <begin position="212"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="domain" description="AAA" evidence="2">
        <location>
            <begin position="485"/>
            <end position="622"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM01073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEF95922.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="732" mass="81301" checksum="63F86BA1BF7A0018" modified="2011-07-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2020-06-17" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBA5</accession>
    <name>F6BBA5_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_1578</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97112.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF97112">
        <property type="protein sequence ID" value="AEF97112"/>
        <property type="gene ID" value="Metig_1578"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_1578"/>
    <dbReference type="HOGENOM" id="CLU_2766086_0_0_2"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF97112.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="69" mass="7966" checksum="5FFBF4CDB6D11401" modified="2011-07-27" version="1">MPLSEILEINLVIFDKMGKMLFRTEASGKLYAIKEKFECLIEKSEEINKTIFFEDEVLFVIPLHGVCPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBE8</accession>
    <name>F6BBE8_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(NiFe)-hydrogenase-3-type complex Eha, EhaM</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_1622</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97155.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799748.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1622"/>
    <dbReference type="EnsemblBacteria" id="AEF97155">
        <property type="protein sequence ID" value="AEF97155"/>
        <property type="gene ID" value="Metig_1622"/>
    </dbReference>
    <dbReference type="GeneID" id="10644495"/>
    <dbReference type="KEGG" id="mig:Metig_1622"/>
    <dbReference type="HOGENOM" id="CLU_133051_0_0_2"/>
    <dbReference type="OMA" id="IDLGLCW"/>
    <dbReference type="OrthoDB" id="101199at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3070.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036606">
        <property type="entry name" value="EhaM-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012056">
        <property type="entry name" value="NiFe-hyd_3_EhaM"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09218">
        <property type="entry name" value="DUF1959"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101332">
        <property type="entry name" value="SSF101332"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF97155.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="138" mass="16279" checksum="ACC22BFD7B05425E" modified="2011-07-27" version="1">MIKIDEKYKKDSLEQKYNIIRDNRYIMEEVIIPISKVLNLPTEEVVEIFVRKCDSATLYELHAYAEQAKMGCLGRRVDIDLGLCWLSDFFGLISKKDADLIRKKVVESHILYKKPYKEALEEGRKLIIQLLKEDLREE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBG0</accession>
    <name>F6BBG0_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Delta-aminolevulinic acid dehydratase</fullName>
            <ecNumber evidence="10">4.2.1.24</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">Metig_1634</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>2 5-aminolevulinate = H(+) + 2 H2O + porphobilinogen</text>
            <dbReference type="Rhea" id="RHEA:24064"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58126"/>
            <dbReference type="ChEBI" id="CHEBI:356416"/>
            <dbReference type="EC" id="4.2.1.24"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; coproporphyrinogen-III from 5-aminolevulinate: step 1/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Homooctamer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the ALAD family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.24" evidence="10"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97167.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799759.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1634"/>
    <dbReference type="EnsemblBacteria" id="AEF97167">
        <property type="protein sequence ID" value="AEF97167"/>
        <property type="gene ID" value="Metig_1634"/>
    </dbReference>
    <dbReference type="GeneID" id="10644507"/>
    <dbReference type="KEGG" id="mig:Metig_1634"/>
    <dbReference type="HOGENOM" id="CLU_035731_0_0_2"/>
    <dbReference type="OMA" id="YQMDYAN"/>
    <dbReference type="OrthoDB" id="31182at2157"/>
    <dbReference type="UniPathway" id="UPA00251">
        <property type="reaction ID" value="UER00318"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004655">
        <property type="term" value="F:porphobilinogen synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006782">
        <property type="term" value="P:protoporphyrinogen IX biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001731">
        <property type="entry name" value="ALAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030656">
        <property type="entry name" value="ALAD_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11458">
        <property type="entry name" value="PTHR11458"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00490">
        <property type="entry name" value="ALAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001415">
        <property type="entry name" value="Porphbilin_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00144">
        <property type="entry name" value="DALDHYDRTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01004">
        <property type="entry name" value="ALAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00169">
        <property type="entry name" value="D_ALA_DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0350">Heme biosynthesis</keyword>
    <keyword evidence="5 10 12" id="KW-0456">Lyase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 10" id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <feature type="active site" description="Schiff-base intermediate with substrate" evidence="7">
        <location>
            <position position="195"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with substrate" evidence="7">
        <location>
            <position position="248"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="120"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="122"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="130"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="233"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR001415-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR001415-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR001415-5"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU004161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AEF97167.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="324" mass="36348" checksum="369C9712206F9AC9" modified="2011-07-27" version="1">MLIRPRRLRKNQKIRDLVRETTLTKNDLIMPIFVDENLKSNERKEIQSMPNQYRFSIEGAVEEAKEIADLGIPAVILFGIPKHKDEVASSAYDKNGVIQKTIKGIKDELGDELLVIADVCLCEYTSHGHCGIVKDGKILNDETLEILSKIALSYAESGADIVAPSDMMDGRVKAIRETLEKHGFDDVAIMSYAAKYASSFYGPFRDAAESAPKFGDRKSYQMDIGNAREAMKEIKLDIEEGADLILVKPALPYLDIIRMARDRFDVPIGGYCVSGEYAMVEAAARNGWLNRESAIFETLLSIKRAGADFIITYWAKEVASWYLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2020-06-17" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBG1</accession>
    <name>F6BBG1_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_1635</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97168.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF97168">
        <property type="protein sequence ID" value="AEF97168"/>
        <property type="gene ID" value="Metig_1635"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_1635"/>
    <dbReference type="HOGENOM" id="CLU_3227808_0_0_2"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF97168.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="43" mass="4818" checksum="D01EC545927729E2" modified="2011-07-27" version="1">MVFILKIFKDSVVTAKGSSSQVADAAASNHKITRHYTLFRKYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBG6</accession>
    <name>F6BBG6_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">NfeD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metig_1641</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97173.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799765.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1641"/>
    <dbReference type="EnsemblBacteria" id="AEF97173">
        <property type="protein sequence ID" value="AEF97173"/>
        <property type="gene ID" value="Metig_1641"/>
    </dbReference>
    <dbReference type="GeneID" id="10644514"/>
    <dbReference type="KEGG" id="mig:Metig_1641"/>
    <dbReference type="HOGENOM" id="CLU_116732_3_0_2"/>
    <dbReference type="OMA" id="LYFPAWG"/>
    <dbReference type="OrthoDB" id="99396at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002810">
        <property type="entry name" value="NfeD-like_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01957">
        <property type="entry name" value="NfeD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="33"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="domain" description="NfeD" evidence="3">
        <location>
            <begin position="51"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEF97173.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="137" mass="15065" checksum="D48C6B0A0CB96BD7" modified="2011-07-27" version="1">MEIGYAFILAGLFMIALETIIPGLYFPAWGIAILIYGVFLIIAPNFALPSAIIAGVVTLYIIHKAVYKKGMDVKIGAEKYIGMEGIMLKDVDEHHYGFVLIDGEKWQAKAKEPIKKGEKVVVVGIEGVSLIVKKKEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBG7</accession>
    <name>F6BBG7_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Band 7 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_1642</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the band 7/mec-2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97174.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1642"/>
    <dbReference type="EnsemblBacteria" id="AEF97174">
        <property type="protein sequence ID" value="AEF97174"/>
        <property type="gene ID" value="Metig_1642"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_1642"/>
    <dbReference type="HOGENOM" id="CLU_024949_2_0_2"/>
    <dbReference type="OMA" id="AMTQQMK"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.479.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001107">
        <property type="entry name" value="Band_7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036013">
        <property type="entry name" value="Band_7/SPFH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018080">
        <property type="entry name" value="Band_7/stomatin-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001972">
        <property type="entry name" value="Stomatin_HflK_HflKC_fam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01145">
        <property type="entry name" value="Band_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00721">
        <property type="entry name" value="STOMATIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00244">
        <property type="entry name" value="PHB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117892">
        <property type="entry name" value="SSF117892"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01270">
        <property type="entry name" value="BAND_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PHB" evidence="2">
        <location>
            <begin position="17"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF97174.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="270" mass="30853" checksum="CDC40C565A5AD6FB" modified="2011-07-27" version="1">MWFWILLGLITLFIITKAVVIVKQYEGGLIFRLGKVIGMLRPGVNLIIPFFDVPIKVDLRTKVVDIPPQEMITKDNAAVRIDAVIYYRVVDVNRAILEVQNYEFAIINLTQTTLRAIIGNMELDEVLNKREHINSILLEILDKETDVWGVKVEKVELREIEPPQDIKDAMTQQMKAERLKRAAILEAEGEKRSRILKAEGIAESYRIEAEGQAKAIKIVAEAAQQYFKDEAQLYKALEVASIVLKDNAKYVISENIMDIAKNFIKKETKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBG9</accession>
    <name>F6BBG9_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1644</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97176.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1644"/>
    <dbReference type="EnsemblBacteria" id="AEF97176">
        <property type="protein sequence ID" value="AEF97176"/>
        <property type="gene ID" value="Metig_1644"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_1644"/>
    <dbReference type="HOGENOM" id="CLU_043916_1_0_2"/>
    <dbReference type="OMA" id="FVGCAML"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002823">
        <property type="entry name" value="DUF112_TM"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01970">
        <property type="entry name" value="TctA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="37"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="93"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="125"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="151"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="176"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="218"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="255"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="297"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="330"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="379"/>
            <end position="396"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF97176.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="397" mass="44604" checksum="30100092A75471FC" modified="2011-07-27" version="1">MPINNLFFLFLGIICGTFTGLFPGVHPNNIVALSFLFIPYFESGYVFFLIGMVVAHYFINYVPTVFLGVPDDETATAVFPMHRLTLRGLGYEAVFLSGFGAFLGIVSSIFVIFVISFLNLDFYRFYSFLKSYLPFILALVLFIIVIFSRSYWALVITLLSGIFGVITLYKNPSFNCTLTAIFTGMFGIPILINNLKTARIPHQIITFPDFKLSFLKSAFFGSVGGFLRIFLPGVGGAQVNYFLSKLIRETDIRNFIVSQGSITLANEVFSIFALFLIGTGRSGVAKAIKNLNIDVDLFQLIPSIIIASSISFVVLLYLSKFILLKMQKINFKKISIFFIFFCTFVVVISSINNHLFYHLTIYLTAISIGILCLKTKTPFSYMMSVLIFPTILYFLSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBH3</accession>
    <name>F6BBH3_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">4-phosphopantoate--beta-alanine ligase</fullName>
            <ecNumber evidence="1">6.3.2.36</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Phosphopantothenate synthetase</fullName>
            <shortName evidence="1">PPS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_1648</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the condensation of (R)-4-phosphopantoate and beta-alanine to 4'-phosphopantothenate in the CoA biosynthesis pathway.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(R)-4-phosphopantoate + ATP + beta-alanine = (R)-4'-phosphopantothenate + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:27930"/>
            <dbReference type="ChEBI" id="CHEBI:10986"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57966"/>
            <dbReference type="ChEBI" id="CHEBI:61294"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.2.36"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; coenzyme A biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the archaeal phosphopantothenate synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="1">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="6.3.2.36" evidence="1"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97180.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799771.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1648"/>
    <dbReference type="EnsemblBacteria" id="AEF97180">
        <property type="protein sequence ID" value="AEF97180"/>
        <property type="gene ID" value="Metig_1648"/>
    </dbReference>
    <dbReference type="GeneID" id="10644521"/>
    <dbReference type="KEGG" id="mig:Metig_1648"/>
    <dbReference type="HOGENOM" id="CLU_078701_0_0_2"/>
    <dbReference type="OMA" id="PKSHPRY"/>
    <dbReference type="OrthoDB" id="76877at2157"/>
    <dbReference type="UniPathway" id="UPA00241"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016881">
        <property type="term" value="F:acid-amino acid ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015937">
        <property type="term" value="P:coenzyme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12640">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02224">
        <property type="entry name" value="PPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002855">
        <property type="entry name" value="PPS/PS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038138">
        <property type="entry name" value="PPS/PS_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40695">
        <property type="entry name" value="PTHR40695"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02006">
        <property type="entry name" value="PPS_PS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004853">
        <property type="entry name" value="UCP004853"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0173">Coenzyme A biosynthesis</keyword>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0436">Ligase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="217"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="16"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="38"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="183"/>
            <end position="185"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="189"/>
            <end position="190"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF97180.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="254" mass="28704" checksum="F4071532B0216ADA" modified="2011-07-27" version="1">MDIPKSHPRYESLLKREKIVEALDKGILAKAGLIAHGRGETFDYLIGEKTTDIALESIKAAAALLVLAENPVISVNGNTVALAKEDVVKLAKELNGKIEVNLFYRTREREEKIKKEFEKEIENGEIRLLGVDDANKQIPNLDSLRGKVSEEGIYTADVVLVPLEDGDRTEALIMMGKKVISIDLNPLSRTARKSTITIVDELTRCLPLLIKYVREYKNKSRDELEDIVNNYNNKENLKRMLNYIADRLKSLEID</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBM4</accession>
    <name>F6BBM4_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Phosphoglucomutase/phosphomannomutase alpha/beta/alpha domain I</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">Metig_0477</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the phosphohexose mutase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96033.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798641.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0477"/>
    <dbReference type="EnsemblBacteria" id="AEF96033">
        <property type="protein sequence ID" value="AEF96033"/>
        <property type="gene ID" value="Metig_0477"/>
    </dbReference>
    <dbReference type="GeneID" id="10643314"/>
    <dbReference type="KEGG" id="mig:Metig_0477"/>
    <dbReference type="HOGENOM" id="CLU_016950_9_2_2"/>
    <dbReference type="OMA" id="HSGEINF"/>
    <dbReference type="OrthoDB" id="58941at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016868">
        <property type="term" value="F:intramolecular transferase activity, phosphotransferases"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005844">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016055">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-I/II/III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005845">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005846">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005843">
        <property type="entry name" value="A-D-PHexomutase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036900">
        <property type="entry name" value="A-D-PHexomutase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016066">
        <property type="entry name" value="A-D-PHexomutase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005841">
        <property type="entry name" value="Alpha-D-phosphohexomutase_SF"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02878">
        <property type="entry name" value="PGM_PMM_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02879">
        <property type="entry name" value="PGM_PMM_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02880">
        <property type="entry name" value="PGM_PMM_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00408">
        <property type="entry name" value="PGM_PMM_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00509">
        <property type="entry name" value="PGMPMM"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53738">
        <property type="entry name" value="SSF53738"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55957">
        <property type="entry name" value="SSF55957"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00710">
        <property type="entry name" value="PGM_PMM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4 6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PGM_PMM_I" evidence="9">
        <location>
            <begin position="2"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="domain" description="PGM_PMM_II" evidence="10">
        <location>
            <begin position="153"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="domain" description="PGM_PMM_III" evidence="11">
        <location>
            <begin position="251"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="domain" description="PGM_PMM_IV" evidence="8">
        <location>
            <begin position="365"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="416"/>
            <end position="444"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF02880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AEF96033.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="447" mass="51779" checksum="FBE4D7794E0F2B51" modified="2011-07-27" version="1">MVFKAYDIRGIYDKELNEKFAYSLGRSIGREYNGKEILVGIDVRMGSKALVKPFIYGLLEEGCRVRYAGVISTPLMYFGTKDNYDLGIILTASHNPPQYTGFKMCDKDAIPLSPIEKIKPIFKNYELNEETTKEIEDIDLEKLKVDIIESYKKFFLKRCEKSDKKITVDFANGATTIAEKEILNELFEDKVFINDYPDGTFPAHQPDTLKMECLKDIIKAVKENNSDLGIIFDGDGDRIGIIDEEGNVLQGDILTAIIAKEILKEYENTKIIYDLRCSKIVLETIEKFGGEGVKCRVGHYFIKKLMHEIDAVFAGELSNHFYFKEIGYFESPLLALNYILKAMEEEGKKLSEIAKEYKKYFHSGEINFKVKDQKYIMEKIKERYKNCKIEEIDGISIYCKDFWFNIRPSNTEPLLRLNLEAEKEDIMKEKVEEIRKYIEELNKDAKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBN0</accession>
    <name>F6BBN0_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">UPF0251 protein Metig_0483</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_0483</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the UPF0251 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96039.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798647.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0483"/>
    <dbReference type="EnsemblBacteria" id="AEF96039">
        <property type="protein sequence ID" value="AEF96039"/>
        <property type="gene ID" value="Metig_0483"/>
    </dbReference>
    <dbReference type="GeneID" id="10643320"/>
    <dbReference type="KEGG" id="mig:Metig_0483"/>
    <dbReference type="HOGENOM" id="CLU_094511_0_1_2"/>
    <dbReference type="OMA" id="VDYLDYT"/>
    <dbReference type="OrthoDB" id="77586at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00674">
        <property type="entry name" value="UPF0251"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013324">
        <property type="entry name" value="RNA_pol_sigma_r3/r4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002852">
        <property type="entry name" value="UPF0251"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37478">
        <property type="entry name" value="PTHR37478"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02001">
        <property type="entry name" value="DUF134"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88659">
        <property type="entry name" value="SSF88659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF96039.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="130" mass="15354" checksum="7B9CDE5C94538A6A" modified="2011-07-27" version="1">MRFRRGRPKIPRLISEEPKLKVFKPQGIPVKDLEIIRLEVDELEAVRLVDLLGYEHEEAARAMGISRRVFWNILKSARKKIADALINGKGIRIEGGYYKIRECKFDEKCGKYKYCRFRLKPCEVFESHEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBP4</accession>
    <name>F6BBP4_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0497</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96053.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798661.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0497"/>
    <dbReference type="EnsemblBacteria" id="AEF96053">
        <property type="protein sequence ID" value="AEF96053"/>
        <property type="gene ID" value="Metig_0497"/>
    </dbReference>
    <dbReference type="GeneID" id="10643334"/>
    <dbReference type="KEGG" id="mig:Metig_0497"/>
    <dbReference type="HOGENOM" id="CLU_137175_0_0_2"/>
    <dbReference type="OMA" id="WALKTAL"/>
    <dbReference type="OrthoDB" id="109427at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="39"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="81"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="106"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="139"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96053.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="164" mass="17740" checksum="1EDB1BB3A67F80A7" modified="2011-07-27" version="1">MASKTNWGMWFVKASVCSLAAGGLFYYAGQQSVTFNEIVTTFASLPLLVVILVEILDKFVDKSDVYKNIYAFVQTKNDASAYFAVFLTAVLAFFGILWLITGSLTLNVGTVSPAVITVAGLLTLYILAPETGDDEIILFLWVGATIATFGKYFTLIPHIPGFGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBR8</accession>
    <name>F6BBR8_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Transposase, IS605 OrfB family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Metig_1666</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97198.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799789.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1666"/>
    <dbReference type="EnsemblBacteria" id="AEF97198">
        <property type="protein sequence ID" value="AEF97198"/>
        <property type="gene ID" value="Metig_1666"/>
    </dbReference>
    <dbReference type="GeneID" id="10644539"/>
    <dbReference type="KEGG" id="mig:Metig_1666"/>
    <dbReference type="HOGENOM" id="CLU_032903_3_4_2"/>
    <dbReference type="OMA" id="MKYSMEV"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032196">
        <property type="term" value="P:transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010095">
        <property type="entry name" value="Transposase_IS605_OrfB_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07282">
        <property type="entry name" value="OrfB_Zn_ribbon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01766">
        <property type="entry name" value="tspaseT_teng_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0815">Transposition</keyword>
    <feature type="domain" description="OrfB_Zn_ribbon" evidence="4">
        <location>
            <begin position="317"/>
            <end position="378"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF07282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEF97198.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="426" mass="50556" checksum="C6F3E58F0A8D6495" modified="2011-07-27" version="1">MQNKNKLPSEIVLTYKIKHNYDLKNLLDKFINLSQKAVDIIWENINWKEKEVKHRFKSKNKYKYYTTTRLIPEIPKDKDFKRELRNYLLNGWNFASHYVDGAIKVAYSTIESWKSNYLDGKRKRNKPTFKRPFVRVKNTLIKYDKENGTIRITIKARKEYLVLDIKNEWFFEKIKGFDIGEIILKDSEALITFKKPLNIFDKKVVIGVDSNLKSLDLFHPEEGWIRIDLSELRRIKNVYDVIIDKLKSIYKKAPKRISILLKKYYNRRKNRVEDFINKLTSQLSKLFPDAIFIFEDLDKPNMYKNSNFNRDLDRTSWREIAKKLEYKSIVFYVNPHYTSKTCPICGSKMESQEGQVVKCEKCGVFNRQFVGSFNIFKRGVKLVKKLLGGVGVLPERSDGNLKTVRFLCPWLGWRSMIYSPMNPEES</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBX7</accession>
    <name>F6BBX7_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Polysaccharide biosynthesis protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Metig_1725</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97257.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799846.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1725"/>
    <dbReference type="EnsemblBacteria" id="AEF97257">
        <property type="protein sequence ID" value="AEF97257"/>
        <property type="gene ID" value="Metig_1725"/>
    </dbReference>
    <dbReference type="GeneID" id="10644599"/>
    <dbReference type="KEGG" id="mig:Metig_1725"/>
    <dbReference type="HOGENOM" id="CLU_648322_0_0_2"/>
    <dbReference type="OMA" id="FIIIDSG"/>
    <dbReference type="OrthoDB" id="41033at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029303">
        <property type="entry name" value="Polysacc_synt_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002797">
        <property type="entry name" value="Polysacc_synth"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01943">
        <property type="entry name" value="Polysacc_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14667">
        <property type="entry name" value="Polysacc_synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="7"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="43"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="83"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="114"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="155"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="208"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="256"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="285"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="316"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="350"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="375"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="domain" description="Polysacc_synt_C" evidence="7">
        <location>
            <begin position="322"/>
            <end position="397"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF14667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEF97257.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="402" mass="44331" checksum="E7DE090AEB4D443B" modified="2011-07-27" version="1">MGLKKDSFYMLLANIYSKGCAYVFFFLMAYLLGTEDFGVLRGILPIMDTLTIFFCSGIPPAMAKFIAEDSYFSLSKYIPTLKIMLLFSLFGGVFILFLRYILGGGYANIPTHVYYALSLTLPFTAFISWSRGILQGTMHIKELSLTWVFEHTSKIIFSVVFAIIFGVFGAILSISTAYFIGGIAGMYFINKSVERLAFKGTFDKNFQLKIISYAVPIALSTASYRLMGDIDSIVIMSLLGAYSNGIYGYAALLSRGVFLFASSIAIPLLPRIAKTRNINYIKKAILLNLIFSLPFVALCFAFPNELLHLFFHVKNPEASICLKILSVSAVFMSIYTISSSALQGLGYAKISLYIILFGIVLNLLLNYILVGEIGVVGGAISTLITSFVILAITIKAILSVKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBY3</accession>
    <name>F6BBY3_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Glycosyl transferase group 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_1731</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97263.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799852.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1731"/>
    <dbReference type="EnsemblBacteria" id="AEF97263">
        <property type="protein sequence ID" value="AEF97263"/>
        <property type="gene ID" value="Metig_1731"/>
    </dbReference>
    <dbReference type="GeneID" id="10644605"/>
    <dbReference type="KEGG" id="mig:Metig_1731"/>
    <dbReference type="HOGENOM" id="CLU_009583_2_3_2"/>
    <dbReference type="OMA" id="HTMAKVK"/>
    <dbReference type="OrthoDB" id="83551at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028098">
        <property type="entry name" value="Glyco_trans_4-like_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13439">
        <property type="entry name" value="Glyco_transf_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glyco_trans_4-like_N" evidence="2">
        <location>
            <begin position="15"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="domain" description="Glycos_transf_1" evidence="1">
        <location>
            <begin position="204"/>
            <end position="363"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF97263.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="390" mass="44455" checksum="610954A033CE9D26" modified="2011-07-27" version="1">MRIAMITWEYPPVIVGGLSIHCKGLAEALVRQGHHVDVITVGYDLPDYENINGVNVHRVKPIKHSYFLIWATIMANFMEKKLGILGIDNYDVIHCHDWMTAFVGINAKHVANKPYVQSIHSTERGRCGGIHSEDSRTINDIEWWSTYESHAIITVSNSIKNEICSIFNTPHDKVNVIYNGINPWEFDIQMDEDEINNFRMHIGVQPHENMILYVGRLVYQKGVEYLIRAFPKILSKYPNSKLVIAGSGDMREYLENLAFQLGCRDRVIFLGFINGNTLKKLYKSSDVCVIPSVYEPFGIVALEAMAAGTPVVVSSVGGLSEIVQHDYNGVWTYPQNPDSIAWGVDRVLSDWEFREYITKNAKRDVYAKYSWDVIAKETVNVYKKVIEMTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2020-08-12" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBZ2</accession>
    <name>F6BBZ2_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0517</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96073.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96073">
        <property type="protein sequence ID" value="AEF96073"/>
        <property type="gene ID" value="Metig_0517"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0517"/>
    <dbReference type="HOGENOM" id="CLU_712940_0_0_2"/>
    <dbReference type="OMA" id="LEVTPWW"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="269"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96073.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="405" mass="47844" checksum="8EC125D88FD540A7" modified="2011-07-27" version="1">MDATFAGSTIPNITNRPQLYEWLERNMERSYKDILDDTKLEPEQNVVKTYILESNTHPKKLSRIIKTGKIELLDEYEFYMLRIGDEGYFFIDAKNPRFWKLYTLQKSEESDKYFNKLISPIKSRLDNLWMPTGDLEKFLKKADYVRGLSTKHDETPFYLDNGVEEIANKLSISGNGESVYKLIEIIQSLSPDDIQEFEKLIEDFQLSNDTKIKKIFNTIKKLHEFKHLMRITKSKIKIVSEDDKDKFVLEDIYYWGKFTVKGTSIEKHNEIVDETIENYEQKIEIIEDSLIDYTSSDWDDRIPLIYEFEKEIEDLKSFVEGLISVKEPFRIWGLAKQVEEDMYYISGVDLHNGDRFSLEVTPWWMRLYLPKGSCGNTALRLLSNIQQTYDSETILEVEKHGERIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-06-02" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBZ8</accession>
    <name>F6BBZ8_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_0524</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96079.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798687.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96079">
        <property type="protein sequence ID" value="AEF96079"/>
        <property type="gene ID" value="Metig_0524"/>
    </dbReference>
    <dbReference type="GeneID" id="10643361"/>
    <dbReference type="KEGG" id="mig:Metig_0524"/>
    <dbReference type="HOGENOM" id="CLU_188099_0_0_2"/>
    <dbReference type="OMA" id="CENIAPE"/>
    <dbReference type="OrthoDB" id="112869at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024254">
        <property type="entry name" value="DUF2540"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010985">
        <property type="entry name" value="Ribbon_hlx_hlx"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10802">
        <property type="entry name" value="DUF2540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47598">
        <property type="entry name" value="SSF47598"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF96079.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="85" mass="10361" checksum="635A3D162DDF9E65" modified="2011-07-27" version="1">MESKRMTFYLCKNVDSRRLRYYLHKLENLKELDPEMFKKVIESKKECRRTITLTEEEIKICEKYGRATNLYLNYVIMVSEDGERV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>F6BC39</accession>
    <name>F6BC39_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Arsenite-activated ATPase ArsA</fullName>
            <ecNumber evidence="2">3.6.3.16</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0566</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.6.3.16" evidence="2"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96120.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798727.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0566"/>
    <dbReference type="EnsemblBacteria" id="AEF96120">
        <property type="protein sequence ID" value="AEF96120"/>
        <property type="gene ID" value="Metig_0566"/>
    </dbReference>
    <dbReference type="GeneID" id="10643404"/>
    <dbReference type="KEGG" id="mig:Metig_0566"/>
    <dbReference type="HOGENOM" id="CLU_040761_4_0_2"/>
    <dbReference type="OMA" id="MDAPYEF"/>
    <dbReference type="OrthoDB" id="84406at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025723">
        <property type="entry name" value="Anion-transp_ATPase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016300">
        <property type="entry name" value="ATPase_ArsA/GET3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10803">
        <property type="entry name" value="PTHR10803"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02374">
        <property type="entry name" value="ArsA_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00345">
        <property type="entry name" value="GET3_arsA_TRC40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ArsA_ATPase" evidence="1">
        <location>
            <begin position="22"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="335" mass="37900" checksum="57715CA21DA62F49" modified="2011-07-27" version="1">MLSKIKDSLKSITTKKLESDGTKYIMFGGKGGVGKTTMSAATGIYCAEQGLKTVIVSTDPAHSLRDAFEQEFGHEPTKVKGFDNLYVVEIDPQKAMEEYKEKLKAQMDENPMLAGMLEEQLEMAALSPGTDESAAFDVFLRYMDSSEFDVVIFDTAPTGHTLRFLGLPEIMDKYMAKMIKFKKQMSGLMKMMKKLMPFGSKDDDIDYDKALEELEKMKKKIEKAREILSNPEKTAFRLVVIPEEMSILESERAMKALEKYGIPIDAVIVNQVIPEDVECEFCKARRALQQKRLEMIKEKFGDKVIAHVPLLKTEAKGVDTLREIAKILYGEEEKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>F6BC40</accession>
    <name>F6BC40_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_0567</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96121.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798728.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0567"/>
    <dbReference type="EnsemblBacteria" id="AEF96121">
        <property type="protein sequence ID" value="AEF96121"/>
        <property type="gene ID" value="Metig_0567"/>
    </dbReference>
    <dbReference type="GeneID" id="10643405"/>
    <dbReference type="KEGG" id="mig:Metig_0567"/>
    <dbReference type="HOGENOM" id="CLU_867711_0_0_2"/>
    <dbReference type="OMA" id="DIIELAC"/>
    <dbReference type="OrthoDB" id="38971at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF96121.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="342" mass="40865" checksum="8684F00DAB903332" modified="2011-07-27" version="1">MIYLPNRGSQRQRVVKIKNSPDSFTNKLSGKIVQILVGRLKKGIDSGQIISLRTKKILSYEEFLPTYNLLERITEENKEVLVKVYEMNIIVEITKEFRNKELLDIFELKEELCSEAEEHIKKYNIEKFLESYTVFCFSDYDNPDEFVKNNKKILGRLLRGQYEDVPEEYIEELIENKLKYSDKDLIILDWDNGIILDKNEDFWEEVDIIELACVRVLNLRVFDYILSERIKYLLQLKLDKLGYFRLRKVSKEVYAQRISYISYLDSVESVLILYGDRYYAELYETLCEIFYVPDWIKRVEKKLEMINDIYSILKEHMVEYYNLILEGTIVALIFIEIIFALL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-06-02" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>F6BC41</accession>
    <name>F6BC41_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0568</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CGI121/TPRKB family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96122.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798729.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96122">
        <property type="protein sequence ID" value="AEF96122"/>
        <property type="gene ID" value="Metig_0568"/>
    </dbReference>
    <dbReference type="GeneID" id="10643406"/>
    <dbReference type="KEGG" id="mig:Metig_0568"/>
    <dbReference type="HOGENOM" id="CLU_1801715_0_0_2"/>
    <dbReference type="OMA" id="FWMEILV"/>
    <dbReference type="OrthoDB" id="90121at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2380.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013926">
        <property type="entry name" value="CGI121/TPRKB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036504">
        <property type="entry name" value="CGI121/TPRKB_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08617">
        <property type="entry name" value="CGI-121"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143870">
        <property type="entry name" value="SSF143870"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96122.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="145" mass="16413" checksum="0255EAEEE0E96EEC" modified="2011-07-27" version="1">MIIRGIKNAKINQDIFKLKLPFQILNAELIATKKHVLHAINQAKTKKNITNSFWMEILVRASAQRQISNAIKLLGAKDGNVCVVCENEEVLSKVLEIIGGEVDDSVLELNEEKEKKIREAFDIKGFGNVVERVCEKIAIMELRKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>F6BC57</accession>
    <name>F6BC57_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">tRNA(Phe) 7-((3-amino-3-carboxypropyl)-4-demethylwyosine(37)-N(4))-methyltransferase</fullName>
            <ecNumber evidence="5">2.1.1.282</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">tRNA wyosine derivatives biosynthesis protein Taw3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">taw3</name>
        <name evidence="7" type="ordered locus">Metig_0584</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">S-adenosyl-L-methionine-dependent methyltransferase that acts as a component of the wyosine derivatives biosynthesis pathway. Probably methylates N-4 position of wybutosine-86 to produce wybutosine-72.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>4-demethyl-7-[(3S)-3-amino-3-carboxypropyl]wyosine(37) in tRNA(Phe) + S-adenosyl-L-methionine = 7-[(3S)-3-amino-3-carboxypropyl]wyosine(37) in tRNA(Phe) + H(+) + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:36635"/>
            <dbReference type="Rhea" id="RHEA-COMP:10378"/>
            <dbReference type="Rhea" id="RHEA-COMP:10379"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:73543"/>
            <dbReference type="ChEBI" id="CHEBI:73550"/>
            <dbReference type="EC" id="2.1.1.282"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the TYW3 family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.282" evidence="5"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96138.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798745.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0584"/>
    <dbReference type="EnsemblBacteria" id="AEF96138">
        <property type="protein sequence ID" value="AEF96138"/>
        <property type="gene ID" value="Metig_0584"/>
    </dbReference>
    <dbReference type="GeneID" id="10643422"/>
    <dbReference type="KEGG" id="mig:Metig_0584"/>
    <dbReference type="HOGENOM" id="CLU_047426_2_0_2"/>
    <dbReference type="OMA" id="GKWHHYA"/>
    <dbReference type="OrthoDB" id="118297at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008175">
        <property type="term" value="F:tRNA methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031591">
        <property type="term" value="P:wybutosine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1960.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00266">
        <property type="entry name" value="TYW3_archaea"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022908">
        <property type="entry name" value="Taw3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003827">
        <property type="entry name" value="tRNA_yW-synthesising"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036602">
        <property type="entry name" value="tRNA_yW-synthesising-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02676">
        <property type="entry name" value="TYW3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111278">
        <property type="entry name" value="SSF111278"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 5" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2 5" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 5" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="TYW3" evidence="6">
        <location>
            <begin position="5"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEF96138.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="194" mass="22565" checksum="E2CC6AB613C0EE7C" modified="2011-07-27" version="1">MFEDDKRKTLMELELAIRSNLVDEEIIPIINKINELDDYYTTSSCIGRCGIMEFPKGKNPKIHSRWLGKWHHYATYDELFEALRKKSDDFELMVFVMNSPILHIAARDINSAKKMVELAIHNGLKASSIKSVSDKRVIVEILGTYKIDAPIGKDGKILVNEDYLKFLLDVGNFKLKKSREILMRWYNKLNELKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>F6BC65</accession>
    <name>F6BC65_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Nitrogenase protein alpha chain</fullName>
            <ecNumber evidence="9">1.18.6.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">Metig_1739</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">This molybdenum-iron protein is part of the nitrogenase complex that catalyzes the key enzymatic reactions in nitrogen fixation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>16 ATP + 16 H2O + N2 + 8 reduced [2Fe-2S]-[ferredoxin] = 16 ADP + 6 H(+) + H2 + 2 NH4(+) + 8 oxidized [2Fe-2S]-[ferredoxin] + 16 phosphate</text>
            <dbReference type="Rhea" id="RHEA:21448"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17997"/>
            <dbReference type="ChEBI" id="CHEBI:18276"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="1.18.6.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>[7Fe-Mo-9S-C-homocitryl] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:30409"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>[8Fe-7S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:21143"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="5">Tetramer of two alpha and two beta chains. Forms complex with the iron protein (nitrogenase component 2).</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the NifD/NifK/NifE/NifN family.</text>
    </comment>
    <dbReference type="EC" id="1.18.6.1" evidence="9"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97271.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799860.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1739"/>
    <dbReference type="EnsemblBacteria" id="AEF97271">
        <property type="protein sequence ID" value="AEF97271"/>
        <property type="gene ID" value="Metig_1739"/>
    </dbReference>
    <dbReference type="GeneID" id="10644613"/>
    <dbReference type="KEGG" id="mig:Metig_1739"/>
    <dbReference type="HOGENOM" id="CLU_025876_1_0_2"/>
    <dbReference type="OMA" id="CGQYSWA"/>
    <dbReference type="OrthoDB" id="10827at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018697">
        <property type="term" value="F:carbonyl sulfide nitrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016163">
        <property type="term" value="F:nitrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009399">
        <property type="term" value="P:nitrogen fixation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000510">
        <property type="entry name" value="Nase/OxRdtase_comp1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005974">
        <property type="entry name" value="Nase_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010143">
        <property type="entry name" value="Nase_comp1_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000318">
        <property type="entry name" value="Nase_comp1_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43457">
        <property type="entry name" value="PTHR43457"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00148">
        <property type="entry name" value="Oxidored_nitro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01284">
        <property type="entry name" value="alt_nitrog_alph"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01862">
        <property type="entry name" value="N2-ase-Ialpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00699">
        <property type="entry name" value="NITROGENASE_1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00090">
        <property type="entry name" value="NITROGENASE_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0408">Iron</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0500">Molybdenum</keyword>
    <keyword evidence="7 8" id="KW-0535">Nitrogen fixation</keyword>
    <keyword evidence="9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Oxidored_nitro" evidence="10">
        <location>
            <begin position="46"/>
            <end position="454"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00002621"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AEF97271.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="482" mass="55053" checksum="0922856FFC262ED1" modified="2011-07-27" version="1">MPFVLLDCDKPIPERMKHTYIYDPEEEIIPACNIKTVPGDMTERGCSFAGARGVVGGPVKDVIHMVHGPIGCAFYTWGTRRNLSDNEFHRRYCFSTDMQESDIVYGGEKKLEKACLEAAEEFPEAKGIFIYATCPTALIGDNLEAVARKVEEKIGKPVFACNSPGFAGCSQSKGHHIFNTEFYRWLKKARERYPEKCLKEEEKTPYDIALIGDYNMDWDLKTIIPLFEKIGCRVVTVFTGNASLDDLFKLPDVKLNVVHCQRSANYIAEMIEDGFNIPKIWVSLFGIEQTAEALRKTAEVLGIDKEKVEEVIKEEVEAIKPKIDFYRSKLEGKKCLVYVGGPRTWHWVRMFKELGVDVVVACCTFAHEDDYEKLNKNLKKAGLKGTLVIDGPNELELEEAVEKYKPDFMLTGLKERYLFRKYGIPTINSHSYEQGPYAGFKGFVNFARDIYKAVCHPIWNVLKEEEAKFEEFKKKKEDDVNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>F6BC96</accession>
    <name>F6BC96_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="4">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">Metig_1770</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>ATP + L-seryl-[protein] = ADP + H(+) + O-phospho-L-seryl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + L-threonyl-[protein] = ADP + H(+) + O-phospho-L-threonyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protein kinase superfamily. RIO-type Ser/Thr kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="4"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97302.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1770"/>
    <dbReference type="EnsemblBacteria" id="AEF97302">
        <property type="protein sequence ID" value="AEF97302"/>
        <property type="gene ID" value="Metig_1770"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_1770"/>
    <dbReference type="HOGENOM" id="CLU_018693_3_3_2"/>
    <dbReference type="OMA" id="EIRCAKV"/>
    <dbReference type="OrthoDB" id="44076at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106310">
        <property type="term" value="F:protein serine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006468">
        <property type="term" value="P:protein phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000687">
        <property type="entry name" value="RIO_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018935">
        <property type="entry name" value="RIO_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="SMART" id="SM00090">
        <property type="entry name" value="RIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="SSF56112"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01245">
        <property type="entry name" value="RIO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 12" id="KW-0418">Kinase</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 12" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6 12" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="RIO" evidence="11">
        <location>
            <begin position="31"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AEF97302.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="272" mass="32327" checksum="1F4ABBBC6314E3AF" modified="2011-07-27" version="1">MHEKEFNLDEIDEFELDREYQKRVIERERKFFEELKTENEVFDKRTLMILFSLLAGKHIDEFIGVISSGKEAVVFSAKKGRRYRAVKIYRVATCDFKTMHKYIQGDPRFHLRKSSRRQIIHAWVEKEFRNLRRAGEVVNAPKAILRRENVLVMELVGKRGVPAPRLKDVEVDYEKFYEIITRDMKKLYCEAELVHGDLSEYNILVKDDKPVYIDFSQSVVTQHPLSKALLIRDVKNICNFFKRKGVDCDYRELYEYITGEKLHPIDEEIAQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCA3</accession>
    <name>F6BCA3_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_1777</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97309.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1777"/>
    <dbReference type="EnsemblBacteria" id="AEF97309">
        <property type="protein sequence ID" value="AEF97309"/>
        <property type="gene ID" value="Metig_1777"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_1777"/>
    <dbReference type="HOGENOM" id="CLU_167318_1_0_2"/>
    <dbReference type="OMA" id="HRSKAEH"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002753">
        <property type="entry name" value="UPF0058"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036519">
        <property type="entry name" value="UPF0058_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42203">
        <property type="entry name" value="PTHR42203"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01893">
        <property type="entry name" value="UPF0058"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140371">
        <property type="entry name" value="SSF140371"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF97309.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="109" mass="12757" checksum="8C226E2F37BC7A75" modified="2011-07-27" version="1">MSQILVSKLLGDYMHKDELIQLHQLLVYMRKYLEKKFGNEINDAFKDYDNLNIHPHHIHRTKSEHIYAIFLLSSSIAKILSENGNVPRSVASRLRDTGEKIGREIARKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCB5</accession>
    <name>F6BCB5_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Probable S-methyl-5'-thioinosine phosphorylase</fullName>
            <ecNumber evidence="3">2.4.2.44</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">5'-methylthioinosine phosphorylase</fullName>
            <shortName evidence="3">MTI phosphorylase</shortName>
            <shortName evidence="3">MTIP</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Metig_1789</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the reversible phosphorylation of S-methyl-5'-thioinosine (MTI) to hypoxanthine and 5-methylthioribose-1-phosphate. Involved in the breakdown of S-methyl-5'-thioadenosine (MTA), a major by-product of polyamine biosynthesis. Catabolism of (MTA) occurs via deamination to MTI and phosphorolysis to hypoxanthine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>phosphate + S-methyl-5'-thioinosine = hypoxanthine + S-methyl-5-thio-alpha-D-ribose 1-phosphate</text>
            <dbReference type="Rhea" id="RHEA:30643"/>
            <dbReference type="ChEBI" id="CHEBI:17368"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:48595"/>
            <dbReference type="ChEBI" id="CHEBI:58533"/>
            <dbReference type="EC" id="2.4.2.44"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Purine metabolism; purine nucleoside salvage.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homotrimer.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="3">Although this enzyme belongs to the family of MTA phosphorylases based on sequence homology, it has been shown that conserved amino acid substitutions in the substrate binding pocket convert the substrate specificity of this enzyme from 6-aminopurines to 6-oxopurines.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the PNP/MTAP phosphorylase family. MTAP subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.44" evidence="3"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97321.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799910.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1789"/>
    <dbReference type="EnsemblBacteria" id="AEF97321">
        <property type="protein sequence ID" value="AEF97321"/>
        <property type="gene ID" value="Metig_1789"/>
    </dbReference>
    <dbReference type="GeneID" id="10644666"/>
    <dbReference type="KEGG" id="mig:Metig_1789"/>
    <dbReference type="HOGENOM" id="CLU_054456_0_2_2"/>
    <dbReference type="OMA" id="MTQCPEA"/>
    <dbReference type="OrthoDB" id="63298at2157"/>
    <dbReference type="UniPathway" id="UPA00606"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017061">
        <property type="term" value="F:S-methyl-5-thioadenosine phosphorylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006166">
        <property type="term" value="P:purine ribonucleoside salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd09010">
        <property type="entry name" value="MTAP_SsMTAPII_like_MTIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1580">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01963">
        <property type="entry name" value="MTAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010044">
        <property type="entry name" value="MTAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000845">
        <property type="entry name" value="Nucleoside_phosphorylase_d"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035994">
        <property type="entry name" value="Nucleoside_phosphorylase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018099">
        <property type="entry name" value="Purine_phosphorylase-2_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42679">
        <property type="entry name" value="PTHR42679"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01048">
        <property type="entry name" value="PNP_UDP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53167">
        <property type="entry name" value="SSF53167"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01694">
        <property type="entry name" value="MTAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01240">
        <property type="entry name" value="PNP_MTAP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3 5" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="3" id="KW-0660">Purine salvage</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PNP_UDP_1" evidence="4">
        <location>
            <begin position="32"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="region of interest" description="Phosphate binding" evidence="3">
        <location>
            <begin position="42"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="3">
        <location>
            <begin position="195"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="8"/>
        </location>
        <ligand>
            <name>phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="171"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="172"/>
        </location>
        <ligand>
            <name>phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </ligand>
    </feature>
    <feature type="site" description="Important for substrate specificity" evidence="3">
        <location>
            <position position="154"/>
        </location>
    </feature>
    <feature type="site" description="Important for substrate specificity" evidence="3">
        <location>
            <position position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEF97321.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="249" mass="28200" checksum="1057AFFACA4CDB5C" modified="2011-07-27" version="1">MIGIIGGTGIASILGKGEEKIIETKYGKAKVLIDGDVVLLFRHGIKHNVPPHKINYRANIYALKQLGVERILAINSVGSLKEEIKPGSFVIPNDFIEFTKCREGTFYDEGKVVHIDMSEPYCPELREILKKILENKKYDYHEGVYVCTEGPRFETKREIEIYKNWGDVVGMTGYPEVVLARELEMCYVSLCNVTNYAAGISKNVLTVDEVLETIKAMEDKILKIVDEFINYDFGERECICKDALKHAVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCG5</accession>
    <name>F6BCG5_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">V-type ATP synthase subunit C</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8 9">V-ATPase subunit C</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">atpC</name>
        <name evidence="11" type="ordered locus">Metig_0626</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 9">Produces ATP from ADP in the presence of a proton gradient across the membrane.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the V-ATPase V0D/AC39 subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96176.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798781.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0626"/>
    <dbReference type="EnsemblBacteria" id="AEF96176">
        <property type="protein sequence ID" value="AEF96176"/>
        <property type="gene ID" value="Metig_0626"/>
    </dbReference>
    <dbReference type="GeneID" id="10643465"/>
    <dbReference type="KEGG" id="mig:Metig_0626"/>
    <dbReference type="HOGENOM" id="CLU_059311_0_0_2"/>
    <dbReference type="OMA" id="WKYTPYS"/>
    <dbReference type="OrthoDB" id="75042at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033179">
        <property type="term" value="C:proton-transporting V-type ATPase, V0 domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042777">
        <property type="term" value="P:proton motive force-driven plasma membrane ATP synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.132.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1690.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00314">
        <property type="entry name" value="ATP_synth_C_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036079">
        <property type="entry name" value="ATPase_su_c/d_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014272">
        <property type="entry name" value="ATPase_V0-cplx_c_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002843">
        <property type="entry name" value="ATPase_V0-cplx_csu/dsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044911">
        <property type="entry name" value="V-type_ATPase_su_c/d_dom_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035067">
        <property type="entry name" value="V-type_ATPase_suC/d"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01992">
        <property type="entry name" value="vATP-synt_AC39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103486">
        <property type="entry name" value="SSF103486"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02923">
        <property type="entry name" value="AhaC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0066">ATP synthesis</keyword>
    <keyword evidence="5 9" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="6 9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="17"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00031704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AEF96176.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="388" mass="43539" checksum="555499C645DA9D9F" modified="2011-07-27" version="1">MDITAIAQFMNMPFDTFMILLILASIVIFLVIIVYILKYVIDLAPFAYTNARIRSMEARLLSEDKLNELIEAGGINELIGFLDDTEYGPYISEVMNELNDPIAIEKALNMHLADVYQTLSNISPDEAKKTLNLLAKKFDIKNIKTLLRAKFVGLNEEETFKLLIPLGTIPVNKLRELSETKSVDEVVSGLEGTEYAPVLSEALAKFEQTGNLLPLELALDKYLLEKLWKTVGIEGKEKDLFKEFVGRMVDIESIKVILRGKADKLPSDVLSEYLVNIGYELAPWKLKELADADSIEGVVSSLEGTVYAQILADAMEEYEKTKSVYAFEKALDKYLVELGKKLSLRKPFGVGPIIGFITAKELEIRNLKIIIKGKLENLPPNQIRTLIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCI3</accession>
    <name>F6BCI3_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Arginine biosynthesis bifunctional protein ArgJ</fullName>
        </recommendedName>
        <domain>
            <recommendedName>
                <fullName evidence="7">Glutamate N-acetyltransferase</fullName>
                <ecNumber evidence="7">2.3.1.35</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="7">Ornithine acetyltransferase</fullName>
                <shortName evidence="7">OATase</shortName>
            </alternativeName>
            <alternativeName>
                <fullName evidence="7">Ornithine transacetylase</fullName>
            </alternativeName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="7">Amino-acid acetyltransferase</fullName>
                <ecNumber evidence="7">2.3.1.1</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="7">N-acetylglutamate synthase</fullName>
                <shortName evidence="7">AGSase</shortName>
            </alternativeName>
        </domain>
        <component>
            <recommendedName>
                <fullName evidence="7">Arginine biosynthesis bifunctional protein ArgJ alpha chain</fullName>
            </recommendedName>
        </component>
        <component>
            <recommendedName>
                <fullName evidence="7">Arginine biosynthesis bifunctional protein ArgJ beta chain</fullName>
            </recommendedName>
        </component>
    </protein>
    <gene>
        <name evidence="7" type="primary">argJ</name>
        <name evidence="9" type="ordered locus">Metig_0644</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes two activities which are involved in the cyclic version of arginine biosynthesis: the synthesis of N-acetylglutamate from glutamate and acetyl-CoA as the acetyl donor, and of ornithine by transacetylation between N(2)-acetylornithine and glutamate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>L-glutamate + N(2)-acetyl-L-ornithine = L-ornithine + N-acetyl-L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:15349"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:44337"/>
            <dbReference type="ChEBI" id="CHEBI:46911"/>
            <dbReference type="ChEBI" id="CHEBI:57805"/>
            <dbReference type="EC" id="2.3.1.35"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>acetyl-CoA + L-glutamate = CoA + H(+) + N-acetyl-L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:24292"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:44337"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="EC" id="2.3.1.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="7">Amino-acid biosynthesis; L-arginine biosynthesis; L-ornithine and N-acetyl-L-glutamate from L-glutamate and N(2)-acetyl-L-ornithine (cyclic): step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="7">Amino-acid biosynthesis; L-arginine biosynthesis; N(2)-acetyl-L-ornithine from L-glutamate: step 1/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Heterotetramer of two alpha and two beta chains.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="miscellaneous">
        <text evidence="7">Some bacteria possess a monofunctional ArgJ, i.e., capable of catalyzing only the fifth step of the arginine biosynthetic pathway.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the ArgJ family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.35" evidence="7"/>
    <dbReference type="EC" id="2.3.1.1" evidence="7"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96194.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0644"/>
    <dbReference type="MEROPS" id="T05.002"/>
    <dbReference type="EnsemblBacteria" id="AEF96194">
        <property type="protein sequence ID" value="AEF96194"/>
        <property type="gene ID" value="Metig_0644"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0644"/>
    <dbReference type="HOGENOM" id="CLU_027172_1_0_2"/>
    <dbReference type="OMA" id="DYVHENS"/>
    <dbReference type="UniPathway" id="UPA00068">
        <property type="reaction ID" value="UER00106"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004042">
        <property type="term" value="F:acetyl-CoA:L-glutamate N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004358">
        <property type="term" value="F:glutamate N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0103045">
        <property type="term" value="F:methione N-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006526">
        <property type="term" value="P:arginine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02152">
        <property type="entry name" value="OAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.340">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01106">
        <property type="entry name" value="ArgJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002813">
        <property type="entry name" value="Arg_biosynth_ArgJ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016117">
        <property type="entry name" value="ArgJ-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042195">
        <property type="entry name" value="ArgJ_beta_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23100">
        <property type="entry name" value="PTHR23100"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01960">
        <property type="entry name" value="ArgJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56266">
        <property type="entry name" value="SSF56266"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00120">
        <property type="entry name" value="ArgJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7 9" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="7" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="7" id="KW-0055">Arginine biosynthesis</keyword>
    <keyword evidence="5 7" id="KW-0068">Autocatalytic cleavage</keyword>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7 9" id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_5023308718" description="Arginine biosynthesis bifunctional protein ArgJ alpha chain" evidence="7">
        <location>
            <begin position="1"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5023308717" description="Arginine biosynthesis bifunctional protein ArgJ beta chain" evidence="7">
        <location>
            <begin position="189"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="86"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="7">
        <location>
            <position position="189"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="150"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="176"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="189"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="271"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="401"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="406"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Involved in the stabilization of negative charge on the oxyanion by the formation of the oxyanion hole" evidence="7">
        <location>
            <position position="112"/>
        </location>
    </feature>
    <feature type="site" description="Involved in the stabilization of negative charge on the oxyanion by the formation of the oxyanion hole" evidence="7">
        <location>
            <position position="113"/>
        </location>
    </feature>
    <feature type="site" description="Cleavage; by autolysis" evidence="7">
        <location>
            <begin position="188"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AEF96194.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="406" mass="44295" checksum="95EDD5C82B9E65CA" modified="2011-07-27" version="1">MVIKMKVIEGGVCAPKGFKANGVKEGKYGVALIVSEKEAVASGVFTTNKVVAHPVILSKEILKNNDKIRAIVANSGNANCFTKNGMEDAKEMIRETAKLLNIKENEILIASTGVIGRKMPMDIIKEKINEAYNLLKKENNSLNAAKAIMTTDSFPKEIAVEFEVNGKKVRIGAIGKGAGMIAPNMLHATMLCFITTDIAIDREDLTRALQNAVDESFNNAVVDGDTSTNDTVFILANGESGVCYEECKEQFENALKFVCTEIAKMIVKDGEGATKFMEVVVKGAKTKEDAKKASMAVVKSLLVKTALFGEDPNWGRIVAAVGYSGAEMDMNKIDVILSDNKREVYLVKNGEQIADEGTEELKLAEEIMKNKEIKIIVNLNIGNEENTAYGCDLSYEYVKINSEYTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-09-29" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCJ7</accession>
    <name>F6BCJ7_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Glycosyl transferase group 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_0658</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96208.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798811.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96208">
        <property type="protein sequence ID" value="AEF96208"/>
        <property type="gene ID" value="Metig_0658"/>
    </dbReference>
    <dbReference type="GeneID" id="10643498"/>
    <dbReference type="KEGG" id="mig:Metig_0658"/>
    <dbReference type="HOGENOM" id="CLU_009583_2_5_2"/>
    <dbReference type="OMA" id="APVPWFP"/>
    <dbReference type="OrthoDB" id="83551at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028098">
        <property type="entry name" value="Glyco_trans_4-like_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13439">
        <property type="entry name" value="Glyco_transf_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glyco_trans_4-like_N" evidence="2">
        <location>
            <begin position="13"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="domain" description="Glycos_transf_1" evidence="1">
        <location>
            <begin position="179"/>
            <end position="324"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF96208.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="346" mass="39527" checksum="9D56F5176AB4ABD2" modified="2011-07-27" version="1">MKVLMPTIYYPFIGGITIHVENLVKHMDDYEFHILTYYSKDYHQKYENVVVHKVPYIPKIRGLSYMINAYKIGKEIIRKEKIDLIHGHYAFPQGVVGGLLKGNLPHILTLHGSDVLKLSKSIIGKPFFNYAISRADKIICVSKFLRDNLGSNFRDKAIVIPNGVDFNLFYEGDDLDYGLFVGSFVKQKGLDVLIDTIKDIDFNFKLIGDGPLFNKIREKIEKENMKHVELLGKKSQTEVAEYMRNCSFLILPSISEGLGMVLLEAMACGKAVIATNVGGIREIVKDNYNGFLIPPNNPKILKEKIEILINDKNLRRKFGKNGKKFSKNFSWENVAKKVRAIYEEML</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCJ8</accession>
    <name>F6BCJ8_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Cobalamin (Vitamin B12) biosynthesis CbiG protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_0659</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96209.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798812.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0659"/>
    <dbReference type="EnsemblBacteria" id="AEF96209">
        <property type="protein sequence ID" value="AEF96209"/>
        <property type="gene ID" value="Metig_0659"/>
    </dbReference>
    <dbReference type="GeneID" id="10643499"/>
    <dbReference type="KEGG" id="mig:Metig_0659"/>
    <dbReference type="HOGENOM" id="CLU_028397_0_0_2"/>
    <dbReference type="OMA" id="GIGCNRG"/>
    <dbReference type="OrthoDB" id="77403at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021744">
        <property type="entry name" value="CbiG_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002750">
        <property type="entry name" value="CobE/GbiG_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036518">
        <property type="entry name" value="CobE/GbiG_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038029">
        <property type="entry name" value="GbiG_N_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01890">
        <property type="entry name" value="CbiG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11760">
        <property type="entry name" value="CbiG_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF159664">
        <property type="entry name" value="SSF159664"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF159672">
        <property type="entry name" value="SSF159672"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CbiG_N" evidence="2">
        <location>
            <begin position="41"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="CbiG_C" evidence="1">
        <location>
            <begin position="193"/>
            <end position="321"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF11760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF96209.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="324" mass="37205" checksum="CFC837C1439CDC25" modified="2011-07-27" version="1">MIKVVYITKRGETLASKIKKILDWYYYESEVIHSKNFKITGNERGFIFIMAMGIVLRKFIDEIKNDKMKDAFVIVCSEDGKYIIPILSNHLGGGNYFSNLIAKNLNANVVFTTATDVNGKVGIDELSKIYFLEIPKRKDILKINRKVLDEKVNLILPKNWKPIGNVSNTYNVSYHNRNYVVVDDDIILKPKKIVVGVGARKNIERHKVYWAIKKTLFLRDIPLWRVDAFATIDVKKDEKGILETVGNFKKPLFIFSRNEVNEVYKFRNDLEKSDFVFKTIGVYGVSEPVSILGVKKLANKNFNEIELILRKFKKNGVSIAISVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-06-02" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCK5</accession>
    <name>F6BCK5_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0666</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96216.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96216">
        <property type="protein sequence ID" value="AEF96216"/>
        <property type="gene ID" value="Metig_0666"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0666"/>
    <dbReference type="HOGENOM" id="CLU_859492_0_0_2"/>
    <dbReference type="OMA" id="GYRKTHF"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1350.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002793">
        <property type="entry name" value="Endonuclease_NucS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011856">
        <property type="entry name" value="tRNA_endonuc-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01939">
        <property type="entry name" value="NucS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96216.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="341" mass="40215" checksum="28C205BE3C47D301" modified="2011-07-27" version="1">MHNITILHTLMEEYTMAYFLISVSNKENLKLCMKYKMAGFTNSVNGFWTFVEVDEGDYISFLYGARVYNLYKVIKKVVLKNANKYGPWSPITFKSGNTYYFPFRLHLQLVRKFEESMVRQEFAYVAENLLLRGGYRKTHFQADEVTFYQVSQMGEKVNDDSRDFEYYPPDDVFTPKITFNNKLQVIPEVFSFKELILQSLVRKFLSKNRDIFCKILQKMGIDGKPQDFEILGEKALPEGHVDIFIKEKHPKGTSKKIVIEVKRNSAKNEDFEQLKKYMDELGNECMGGILIAKSFSKRLSIPQENILCLTYKFENVGRNNLYTYDELLTRLQLQFLERWRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCP4</accession>
    <name>F6BCP4_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 8">Acetolactate synthase</fullName>
            <ecNumber evidence="4 8">2.2.1.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">Metig_0705</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>H(+) + 2 pyruvate = (2S)-2-acetolactate + CO2</text>
            <dbReference type="Rhea" id="RHEA:25249"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:58476"/>
            <dbReference type="EC" id="2.2.1.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="8">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
        <text evidence="8">Binds 1 thiamine pyrophosphate per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 8">Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 1/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 8">Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 1/4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the TPP enzyme family.</text>
    </comment>
    <dbReference type="EC" id="2.2.1.6" evidence="4 8"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96255.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0705"/>
    <dbReference type="EnsemblBacteria" id="AEF96255">
        <property type="protein sequence ID" value="AEF96255"/>
        <property type="gene ID" value="Metig_0705"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0705"/>
    <dbReference type="HOGENOM" id="CLU_013748_1_2_2"/>
    <dbReference type="OMA" id="CFGTSGP"/>
    <dbReference type="UniPathway" id="UPA00047">
        <property type="reaction ID" value="UER00055"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00049">
        <property type="reaction ID" value="UER00059"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003984">
        <property type="term" value="F:acetolactate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009097">
        <property type="term" value="P:isoleucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044272">
        <property type="term" value="P:sulfur compound biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009099">
        <property type="term" value="P:valine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd02015">
        <property type="entry name" value="TPP_AHAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012846">
        <property type="entry name" value="Acetolactate_synth_lsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039368">
        <property type="entry name" value="AHAS_TPP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012000">
        <property type="entry name" value="Thiamin_PyroP_enz_cen_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012001">
        <property type="entry name" value="Thiamin_PyroP_enz_TPP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000399">
        <property type="entry name" value="TPP-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045229">
        <property type="entry name" value="TPP_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011766">
        <property type="entry name" value="TPP_enzyme-bd_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18968">
        <property type="entry name" value="PTHR18968"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02775">
        <property type="entry name" value="TPP_enzyme_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00205">
        <property type="entry name" value="TPP_enzyme_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02776">
        <property type="entry name" value="TPP_enzyme_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="SSF52467"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="SSF52518"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00118">
        <property type="entry name" value="acolac_lg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00187">
        <property type="entry name" value="TPP_ENZYMES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="7 8" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0786">Thiamine pyrophosphate</keyword>
    <keyword evidence="6 8 12" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="TPP_enzyme_N" evidence="11">
        <location>
            <begin position="21"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="domain" description="TPP_enzyme_M" evidence="9">
        <location>
            <begin position="213"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="domain" description="TPP_enzyme_C" evidence="10">
        <location>
            <begin position="413"/>
            <end position="560"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003591"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF02776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AEF96255.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="608" mass="66601" checksum="D15D8A5F23F69CED" modified="2011-07-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCQ2</accession>
    <name>F6BCQ2_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 9">Peptide chain release factor subunit 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8 9">Translation termination factor aRF1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">prf1</name>
        <name evidence="11" type="ordered locus">Metig_0713</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 9">Directs the termination of nascent peptide synthesis (translation) in response to the termination codons UAA, UAG and UGA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4 9">Heterodimer of two subunits, one of which binds GTP.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the eukaryotic release factor 1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96263.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798866.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0713"/>
    <dbReference type="EnsemblBacteria" id="AEF96263">
        <property type="protein sequence ID" value="AEF96263"/>
        <property type="gene ID" value="Metig_0713"/>
    </dbReference>
    <dbReference type="GeneID" id="10643553"/>
    <dbReference type="KEGG" id="mig:Metig_0713"/>
    <dbReference type="HOGENOM" id="CLU_035759_3_0_2"/>
    <dbReference type="OMA" id="GQEMEVV"/>
    <dbReference type="OrthoDB" id="32191at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016149">
        <property type="term" value="F:translation release factor activity, codon specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.960.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00424">
        <property type="entry name" value="Rel_fact_arch_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042226">
        <property type="entry name" value="eFR1_2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005140">
        <property type="entry name" value="eRF1_1_Pelota"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024049">
        <property type="entry name" value="eRF1_1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005141">
        <property type="entry name" value="eRF1_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005142">
        <property type="entry name" value="eRF1_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029064">
        <property type="entry name" value="L30e-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020918">
        <property type="entry name" value="Peptide_chain-rel_aRF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004403">
        <property type="entry name" value="Peptide_chain-rel_eRF1/aRF1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10113">
        <property type="entry name" value="PTHR10113"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03463">
        <property type="entry name" value="eRF1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03464">
        <property type="entry name" value="eRF1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03465">
        <property type="entry name" value="eRF1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01194">
        <property type="entry name" value="eRF1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55315">
        <property type="entry name" value="SSF55315"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55481">
        <property type="entry name" value="SSF55481"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03676">
        <property type="entry name" value="aRF1/eRF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 9" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="eRF1_1" evidence="10">
        <location>
            <begin position="1"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00019723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00031168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM01194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AEF96263.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="421" mass="47889" checksum="923888729853DD18" modified="2011-07-27" version="1">MEEDKSKELYMFKKTLQELKSKKGKGTELISLYIPAGRRISDVTQYLREELSQSSNIKSKSTRKNVQSAIEVILQRLKLLKDPLEKGVIIFAGMVPRSGPGTEKMETYVLIPPEPIKTFIYRCDSQFYTEPLEEFLEEKDTYGVILIDRSEATIATVKGKNIQIHKRLTSGVPGKFKAGGQSARRLERLIDDAAHQFMVRVGEHATEVFLPLLEKKQLRGILVGGPGNTKNEFVEGDYLHHELKKIVLDTFDLCYTEEFGIRELLSKAEHLLKDLELMKEREVVQRFFKELIKEDGGLAAYGEKEVLEALIMGAVDTLIVSEDINMVRVKIKCNNCEYTEELNIKKDELPKLEEALKEKTCPQCEGALVIEETKDFIEYLSELCEMSGTKLCIVSRETEEGAQISKAFKGLAAILRFKLHQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCS5</accession>
    <name>F6BCS5_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ABC transporter related protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Metig_0738</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96286.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798889.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0738"/>
    <dbReference type="EnsemblBacteria" id="AEF96286">
        <property type="protein sequence ID" value="AEF96286"/>
        <property type="gene ID" value="Metig_0738"/>
    </dbReference>
    <dbReference type="GeneID" id="10643577"/>
    <dbReference type="KEGG" id="mig:Metig_0738"/>
    <dbReference type="HOGENOM" id="CLU_000604_48_1_2"/>
    <dbReference type="OMA" id="IMFDEPD"/>
    <dbReference type="OrthoDB" id="44302at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03217">
        <property type="entry name" value="ABC_FeS_Assembly"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010230">
        <property type="entry name" value="FeS-cluster_ATPase_SufC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43204">
        <property type="entry name" value="PTHR43204"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01978">
        <property type="entry name" value="sufC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="2"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="34"/>
            <end position="41"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEF96286.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="243" mass="27341" checksum="B9C31FFB7C817451" modified="2011-07-27" version="1">MLEIHDLSVKIGDKTILKDLNLKINEGEVHVLFGPNGTGKSTLIGTILGNPKYEVVGGEMIFKGEDITNLPMHERAKRGIGILFQTPPAIQGVKLLDLLKIIAKRDEKEILKMAKRLKFENFLDRDVNVGFSGGERKKSEILQLYAQNPDFIMFDEPDSGVDVENVELIGRIINEMLDKNKEPDERRKSGLIVTHLGYILNFIDADKAHVLYNGKIILSGKPKEILNRIVEEGYEGVYNDSTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>F6BD08</accession>
    <name>F6BD08_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Enolase</fullName>
            <ecNumber evidence="7">4.2.1.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">2-phospho-D-glycerate hydro-lyase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">2-phosphoglycerate dehydratase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">eno</name>
        <name evidence="12" type="ordered locus">Metig_0824</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the reversible conversion of 2-phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>(2R)-2-phosphoglycerate = H2O + phosphoenolpyruvate</text>
            <dbReference type="Rhea" id="RHEA:10164"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:58289"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="EC" id="4.2.1.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 7">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="activity regulation">
        <text evidence="7">The covalent binding to the substrate causes inactivation of the enzyme, and possibly serves as a signal for the export of the protein.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 7">Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Secreted</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Cell surface</location>
        </subcellularLocation>
        <text evidence="7">Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the cell surface.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 7">Belongs to the enolase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.11" evidence="7"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96369.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798971.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0824"/>
    <dbReference type="EnsemblBacteria" id="AEF96369">
        <property type="protein sequence ID" value="AEF96369"/>
        <property type="gene ID" value="Metig_0824"/>
    </dbReference>
    <dbReference type="GeneID" id="10643665"/>
    <dbReference type="KEGG" id="mig:Metig_0824"/>
    <dbReference type="HOGENOM" id="CLU_031223_2_1_2"/>
    <dbReference type="OMA" id="EFMIIPV"/>
    <dbReference type="OrthoDB" id="23221at2157"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00187"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000015">
        <property type="term" value="C:phosphopyruvate hydratase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004634">
        <property type="term" value="F:phosphopyruvate hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03313">
        <property type="entry name" value="enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00318">
        <property type="entry name" value="Enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000941">
        <property type="entry name" value="Enolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036849">
        <property type="entry name" value="Enolase-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029017">
        <property type="entry name" value="Enolase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020810">
        <property type="entry name" value="Enolase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020809">
        <property type="entry name" value="Enolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020811">
        <property type="entry name" value="Enolase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11902">
        <property type="entry name" value="PTHR11902"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00113">
        <property type="entry name" value="Enolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03952">
        <property type="entry name" value="Enolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001400">
        <property type="entry name" value="Enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00148">
        <property type="entry name" value="ENOLASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00002">
        <property type="entry name" value="enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01192">
        <property type="entry name" value="Enolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01193">
        <property type="entry name" value="Enolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51604">
        <property type="entry name" value="SSF51604"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54826">
        <property type="entry name" value="SSF54826"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01060">
        <property type="entry name" value="eno"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00164">
        <property type="entry name" value="ENOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 7" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="6 7 12" id="KW-0456">Lyase</keyword>
    <keyword evidence="4 7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0964">Secreted</keyword>
    <feature type="domain" description="Enolase_N" evidence="11">
        <location>
            <begin position="11"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="domain" description="Enolase_C" evidence="10">
        <location>
            <begin position="145"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="7 9">
        <location>
            <begin position="367"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="7 8">
        <location>
            <position position="213"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="7 8">
        <location>
            <position position="340"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7 9">
        <location>
            <position position="161"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7 9">
        <location>
            <position position="170"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="248"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7 9">
        <location>
            <position position="289"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7 9">
        <location>
            <position position="315"/>
        </location>
    </feature>
    <feature type="binding site" description="covalent; in inhibited form" evidence="7">
        <location>
            <position position="340"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7 9">
        <location>
            <position position="391"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00318"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR001400-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR001400-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM01192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM01193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AEF96369.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="428" mass="46475" checksum="FB1E86C0BED7C0DC" modified="2011-07-27" version="1">MLKHVDDSFEIYDIHAREVLDSRGNPTVEVEVLTLGRGIGSAIVPSGASTGTHEALELRDKSKRFGGKGVLEAVDNVNSIIKPELIGYDSRMQREIDTIMIELDGTENKSKLGANAILAVSMAVAKAAADTASLPLYKYLGGCNSFVMPVPMMNVINGGKHAGNALDFQEFMIMPVGADSIAEAIRIGAETYQALKKVILEKYGKNAINVGDEGGFAPPIEDARDALNLLAEAAKLAGYENDIVFALDCAASEFYKDGYYIVKGEKLTREQLIELYKELTDEYPIVSIEDPLHEEDFEGFAMVTKELKGVQIVGDDLFVTNINRLRKGVEVGAANALLLKVNQIGTLSEAIDAANLAFRNGYGVIVSHRSGETEDTTIADLAVALNAGQIKTGAPARGERTAKYNQLIRIEEELGYPKYAGRYFRVPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F6BD53</accession>
    <name>F6BD53_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Hydrogenase assembly chaperone hypC/hupF</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_0870</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the HupF/HypC family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96414.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799016.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0870"/>
    <dbReference type="EnsemblBacteria" id="AEF96414">
        <property type="protein sequence ID" value="AEF96414"/>
        <property type="gene ID" value="Metig_0870"/>
    </dbReference>
    <dbReference type="GeneID" id="10643714"/>
    <dbReference type="KEGG" id="mig:Metig_0870"/>
    <dbReference type="HOGENOM" id="CLU_159381_2_2_2"/>
    <dbReference type="OMA" id="MCLAIPC"/>
    <dbReference type="OrthoDB" id="119258at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019812">
        <property type="entry name" value="Hydgase_assmbl_chp_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001109">
        <property type="entry name" value="Hydrogenase_HupF/HypC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35177">
        <property type="entry name" value="PTHR35177"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01455">
        <property type="entry name" value="HupF_HypC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00445">
        <property type="entry name" value="HUPFHYPC"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00074">
        <property type="entry name" value="hypC_hupF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01097">
        <property type="entry name" value="HUPF_HYPC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="59"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF96414.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="88" mass="10080" checksum="7A95ED4B3F741E7B" modified="2011-07-27" version="1">MCLAIPCKVIEIIEEDGEKYAIAEYKGVKQKAKLTLLENVKVGDYILIHTGYALEVMSEEDAKLSLEAWEELFNALEEMDKEKEKINE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>F6BD55</accession>
    <name>F6BD55_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Transposase, IS605 OrfB family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Metig_0873</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96416.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799018.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0873"/>
    <dbReference type="EnsemblBacteria" id="AEF96416">
        <property type="protein sequence ID" value="AEF96416"/>
        <property type="gene ID" value="Metig_0873"/>
    </dbReference>
    <dbReference type="GeneID" id="10643717"/>
    <dbReference type="KEGG" id="mig:Metig_0873"/>
    <dbReference type="HOGENOM" id="CLU_032903_3_4_2"/>
    <dbReference type="OMA" id="YSILNSW"/>
    <dbReference type="OrthoDB" id="75656at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032196">
        <property type="term" value="P:transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010095">
        <property type="entry name" value="Transposase_IS605_OrfB_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07282">
        <property type="entry name" value="OrfB_Zn_ribbon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01766">
        <property type="entry name" value="tspaseT_teng_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0815">Transposition</keyword>
    <feature type="domain" description="OrfB_Zn_ribbon" evidence="4">
        <location>
            <begin position="317"/>
            <end position="378"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF07282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEF96416.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="434" mass="51071" checksum="F4903432BD540D04" modified="2011-07-27" version="1">MQNKNKLPSEIVLTYKIKHNYDLKNLLDGFMSTSQKAVDIIWENINWKEKQVKHRFKSKNKYKYYTTTRLIPEIPKDKDFKRELRNYLLNGWNFASHYVDGAIKVAYSTIESWKSNYLDGKRKRNKPTFKRPFVRVKNTLIKYDKENGTIRITIKARKEYLVLDIKNEWFFEKIRGFDIGEIILKDNEALITFKKPLNIFDKKVVIGVDSNLKSLDLFHPEEGWIRVDLSELHRIKNVYDVIIDKLKSIYKKAPKRIGILLKKYYNRRKNRVEDFINKLTSQLSKLFPNAIFIFEDLDKLNMYKNSNFNRDLDRTSWREIAKKLEYKSIVFYVNPHYTSKTCPICGSRMESQEGQVVTCEKCGVFNRQFVGSFNIFKRGVKLVKKLLGGVGVPVTGVEVDDLLSNEPRGELRPMSPKSIVRVNLSGSTFIHIYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>F6BD59</accession>
    <name>F6BD59_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Chromosome segregation and condensation protein ScpA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0877</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96420.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799022.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0877"/>
    <dbReference type="EnsemblBacteria" id="AEF96420">
        <property type="protein sequence ID" value="AEF96420"/>
        <property type="gene ID" value="Metig_0877"/>
    </dbReference>
    <dbReference type="GeneID" id="10643721"/>
    <dbReference type="KEGG" id="mig:Metig_0877"/>
    <dbReference type="HOGENOM" id="CLU_070251_1_1_2"/>
    <dbReference type="OMA" id="GKVDPWN"/>
    <dbReference type="OrthoDB" id="81037at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.580">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003768">
        <property type="entry name" value="ScpA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023093">
        <property type="entry name" value="ScpA-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33969">
        <property type="entry name" value="PTHR33969"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02616">
        <property type="entry name" value="SMC_ScpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="115"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96420.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="228" mass="27012" checksum="6AE7317E05D57BD5" modified="2011-07-27" version="1">MEFELWVRMIKESIEKKNVDPWNINIAEIADLYMSKIKELKKFDIRLSADVILVGGILLRMKSEVLYGECEVIDDEYEEEFYEDYDDYYGYDEDNEDVKITPPVRRPPAKINKEKITLDDLIKTLRDELEKVKKKKTKKSKSSKKSEVILGDVQDIVEELIEEEDISDIIDKLLDDLRREKIIVFQKRFSSKEDKVKYFLPSLYLANDGKVELIQEELFKDLIVKLKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>F6BD90</accession>
    <name>F6BD90_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">50S ribosomal protein L14</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">rpl14</name>
        <name evidence="5" type="ordered locus">Metig_0908</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Binds to 23S rRNA. Forms part of two intersubunit bridges in the 70S ribosome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Part of the 50S ribosomal subunit. Forms a cluster with proteins L3 and L24e, part of which may contact the 16S rRNA in 2 intersubunit bridges.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 4">Belongs to the universal ribosomal protein uL14 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96451.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799053.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0908"/>
    <dbReference type="EnsemblBacteria" id="AEF96451">
        <property type="protein sequence ID" value="AEF96451"/>
        <property type="gene ID" value="Metig_0908"/>
    </dbReference>
    <dbReference type="GeneID" id="10643752"/>
    <dbReference type="KEGG" id="mig:Metig_0908"/>
    <dbReference type="HOGENOM" id="CLU_095071_3_1_2"/>
    <dbReference type="OMA" id="AKEVLCI"/>
    <dbReference type="OrthoDB" id="91632at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.150.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01367">
        <property type="entry name" value="Ribosomal_L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036853">
        <property type="entry name" value="Ribosomal_L14_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000218">
        <property type="entry name" value="Ribosomal_L14P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019971">
        <property type="entry name" value="Ribosomal_L14P_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019972">
        <property type="entry name" value="Ribosomal_L14P_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11761">
        <property type="entry name" value="PTHR11761"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00238">
        <property type="entry name" value="Ribosomal_L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01374">
        <property type="entry name" value="Ribosomal_L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50193">
        <property type="entry name" value="SSF50193"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03673">
        <property type="entry name" value="uL14_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00049">
        <property type="entry name" value="RIBOSOMAL_L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="1 3 4" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3" id="KW-0699">rRNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEF96451.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="132" mass="14339" checksum="331A04374DB5A449" modified="2011-07-27" version="1">MKGHGSKVTRALPNGARLVCADNTGARELEIIAVKNYKGVARRLPSAGVGDMVFVSVKKGTPEMRKQVFPAIIIRQKKEIRRPDGTRVKFEDNAAVIVTPDGNPKGSEIKGPVAKEAAERWPGISRLARIIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDA4</accession>
    <name>F6BDA4_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Chromosome partition protein Smc</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">smc</name>
        <name evidence="5" type="ordered locus">Metig_0922</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Required for chromosome condensation and partitioning.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="3">Contains large globular domains required for ATP hydrolysis at each terminus and a third globular domain forming a flexible hinge near the middle of the molecule. These domains are separated by coiled-coil structures.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the SMC family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96465.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799067.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0922"/>
    <dbReference type="EnsemblBacteria" id="AEF96465">
        <property type="protein sequence ID" value="AEF96465"/>
        <property type="gene ID" value="Metig_0922"/>
    </dbReference>
    <dbReference type="GeneID" id="10643766"/>
    <dbReference type="KEGG" id="mig:Metig_0922"/>
    <dbReference type="HOGENOM" id="CLU_001042_2_2_2"/>
    <dbReference type="OMA" id="HNKIAME"/>
    <dbReference type="OrthoDB" id="1023at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030261">
        <property type="term" value="P:chromosome condensation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007062">
        <property type="term" value="P:sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01894">
        <property type="entry name" value="Smc_prok"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003395">
        <property type="entry name" value="RecF/RecN/SMC_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024704">
        <property type="entry name" value="SMC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010935">
        <property type="entry name" value="SMC_hinge"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036277">
        <property type="entry name" value="SMC_hinge_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011890">
        <property type="entry name" value="SMC_prok"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06470">
        <property type="entry name" value="SMC_hinge"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02463">
        <property type="entry name" value="SMC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005719">
        <property type="entry name" value="SMC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00968">
        <property type="entry name" value="SMC_hinge"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75553">
        <property type="entry name" value="SSF75553"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02169">
        <property type="entry name" value="SMC_prok_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02168">
        <property type="entry name" value="SMC_prok_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1 3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SMC hinge" evidence="4">
        <location>
            <begin position="535"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="181"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="683"/>
            <end position="954"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="1000"/>
            <end position="1037"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="32"/>
            <end position="39"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEF96465.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="1181" mass="137692" checksum="1CD81E7AD40F2188" modified="2011-07-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2020-06-17" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDC9</accession>
    <name>F6BDC9_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_0947</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96490.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96490">
        <property type="protein sequence ID" value="AEF96490"/>
        <property type="gene ID" value="Metig_0947"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0947"/>
    <dbReference type="HOGENOM" id="CLU_3362562_0_0_2"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF96490.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="35" mass="4357" checksum="3A0B73686B462BD2" modified="2011-07-27" version="1">MHVYDEKVYVYYVDRLITYNDLFVFGNKVFELLIH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-06-02" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDE6</accession>
    <name>F6BDE6_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF2341 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0964</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96507.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799109.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96507">
        <property type="protein sequence ID" value="AEF96507"/>
        <property type="gene ID" value="Metig_0964"/>
    </dbReference>
    <dbReference type="GeneID" id="10643810"/>
    <dbReference type="KEGG" id="mig:Metig_0964"/>
    <dbReference type="HOGENOM" id="CLU_525443_0_0_2"/>
    <dbReference type="OrthoDB" id="44722at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018765">
        <property type="entry name" value="DUF2341"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10102">
        <property type="entry name" value="DUF2341"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF2341" evidence="1">
        <location>
            <begin position="225"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF10102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96507.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="518" mass="58514" checksum="C25B3797E00A1E77" modified="2011-07-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-06-02" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDG6</accession>
    <name>F6BDG6_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_0985</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96527.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799129.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96527">
        <property type="protein sequence ID" value="AEF96527"/>
        <property type="gene ID" value="Metig_0985"/>
    </dbReference>
    <dbReference type="GeneID" id="10643832"/>
    <dbReference type="KEGG" id="mig:Metig_0985"/>
    <dbReference type="HOGENOM" id="CLU_077467_2_0_2"/>
    <dbReference type="OMA" id="RHNPQFN"/>
    <dbReference type="OrthoDB" id="125462at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007438">
        <property type="entry name" value="DUF488"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014519">
        <property type="entry name" value="UCP024492"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04343">
        <property type="entry name" value="DUF488"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF024492">
        <property type="entry name" value="UCP024492"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF96527.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="165" mass="19215" checksum="EFE662681D857BC8" modified="2011-07-27" version="1">MPPIDCIVESFIETHGKLINAKTPIIHTIGYEGRSLDEFIQTLKEHNIHAVVDVRELPLSRKKGFSKTRLSNILNNNGIEYTHMKNLGAPKELRNGLKSKKMTYKEFSILYKKHLNEQADSLKKLLEYALSKSCVLMCYERDWKVCHRRIIVEYMRNAGFEVIHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDI7</accession>
    <name>F6BDI7_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_1006</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96548.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1006"/>
    <dbReference type="EnsemblBacteria" id="AEF96548">
        <property type="protein sequence ID" value="AEF96548"/>
        <property type="gene ID" value="Metig_1006"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_1006"/>
    <dbReference type="HOGENOM" id="CLU_102155_0_0_2"/>
    <dbReference type="OMA" id="FVANHYK"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007368">
        <property type="entry name" value="DUF434"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041652">
        <property type="entry name" value="DUF5616"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04256">
        <property type="entry name" value="DUF434"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18481">
        <property type="entry name" value="DUF5616"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF434" evidence="1">
        <location>
            <begin position="15"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="domain" description="DUF5616" evidence="2">
        <location>
            <begin position="82"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF18481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF96548.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="221" mass="25768" checksum="E9A0759CAE54DCF1" modified="2011-07-27" version="1">MRGSMSTKKVDERIIEEAKKDFKYLIDRGYRKEYALNVVCNHYRIPKNERIKIIRTVHSEEEIKIIKSKLISIEDLKKLKSKEIEINIDGYNVLIGVEAFLKGRVILCDDGIYRDFEGVYGKYKMSEYTEKSLNLLLKLFKKHNIKPTFYFDAQVSKSGELSKMTKNFMEENNINGDAICTKHCDKTLKEKEVVATSDTIIIKSPKVRYVVDLIKELKVVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDK9</accession>
    <name>F6BDK9_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">GTP-dependent dephospho-CoA kinase</fullName>
            <ecNumber evidence="3">2.7.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Dephospho-coenzyme A kinase</fullName>
            <shortName evidence="3">DPCK</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metig_1028</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the GTP-dependent phosphorylation of the 3'-hydroxyl group of dephosphocoenzyme A to form coenzyme A (CoA).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>3'-dephospho-CoA + GTP = CoA + GDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:61156"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57328"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Cofactor biosynthesis; coenzyme A biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the GTP-dependent DPCK family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.-" evidence="3"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96570.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799172.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1028"/>
    <dbReference type="EnsemblBacteria" id="AEF96570">
        <property type="protein sequence ID" value="AEF96570"/>
        <property type="gene ID" value="Metig_1028"/>
    </dbReference>
    <dbReference type="GeneID" id="10643882"/>
    <dbReference type="KEGG" id="mig:Metig_1028"/>
    <dbReference type="HOGENOM" id="CLU_120795_1_0_2"/>
    <dbReference type="OMA" id="AIYDHKT"/>
    <dbReference type="OrthoDB" id="116732at2157"/>
    <dbReference type="UniPathway" id="UPA00241"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015937">
        <property type="term" value="P:coenzyme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00590">
        <property type="entry name" value="Dephospho_CoA_kinase_GTP_dep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007164">
        <property type="entry name" value="GTP-dep_dephospho-CoA_kin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40732">
        <property type="entry name" value="PTHR40732"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04019">
        <property type="entry name" value="DUF359"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006533">
        <property type="entry name" value="UCP006533"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0173">Coenzyme A biosynthesis</keyword>
    <keyword evidence="1 3" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEF96570.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="158" mass="18142" checksum="F1AD45CAFA8AC8ED" modified="2011-07-27" version="1">MYILTDELRKLLKKPFGKVYKELPPINGKVVSVGDITTKTLLSKGIIPNLSIFDLKTKRDIKVDIEHKFKKTFEVENPPGCISDEAIEKIKYLANINDKDIALLVKGEEDLLVIPVIKYFPENTIVLYGQPNEGIVFLKITQELKNQIDEILKMMERR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-06-02" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDM2</accession>
    <name>F6BDM2_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PocR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1042</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96583.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799185.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96583">
        <property type="protein sequence ID" value="AEF96583"/>
        <property type="gene ID" value="Metig_1042"/>
    </dbReference>
    <dbReference type="GeneID" id="10643896"/>
    <dbReference type="KEGG" id="mig:Metig_1042"/>
    <dbReference type="HOGENOM" id="CLU_876062_0_0_2"/>
    <dbReference type="OrthoDB" id="133391at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018771">
        <property type="entry name" value="PocR_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10114">
        <property type="entry name" value="PocR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PocR" evidence="1">
        <location>
            <begin position="13"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF10114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96583.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="317" mass="36860" checksum="F67459FEB16010A1" modified="2011-07-27" version="1">MELEKIQKINILVLKPISELLKANVATFDKNGKVIQPFYFGNEICKMIKTHEIGRNICQNSHKRVLNSAKLKNNTVKKSIIEKELSKITPIAYPDDEKGPIIIDYCDAGLLKVLYPIKNNGEIVGYTGICGCFKEGEEEYSLKKLASVSEVISVDKDKLLNLAKDEIRSLSENEIKVVLELVKNMIKYDIDFKKLSACLSKKELSEEDEEYILKFLEYFVLPTAEIINSNLMFFDNELNALFVMDYDKFSFVMEQMDYNKDRIRKLDKPLFLEQEKMFIIPIHKLYLVGTISENNKYTTEEIKKFVYVIYQYFQIVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDM3</accession>
    <name>F6BDM3_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcriptional regulator, TrmB</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1043</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96584.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799186.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1043"/>
    <dbReference type="EnsemblBacteria" id="AEF96584">
        <property type="protein sequence ID" value="AEF96584"/>
        <property type="gene ID" value="Metig_1043"/>
    </dbReference>
    <dbReference type="GeneID" id="10643897"/>
    <dbReference type="KEGG" id="mig:Metig_1043"/>
    <dbReference type="HOGENOM" id="CLU_140786_1_0_2"/>
    <dbReference type="OMA" id="EDLMRCI"/>
    <dbReference type="OrthoDB" id="96961at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002831">
        <property type="entry name" value="Tscrpt_reg_TrmB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01978">
        <property type="entry name" value="TrmB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TrmB" evidence="1">
        <location>
            <begin position="19"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96584.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="115" mass="13919" checksum="F62CF1CD8BEFA5D3" modified="2011-07-27" version="1">MDNLIVARMQKFTIEDLMRCILGLQEIEIRIYFELLDMKEATVMEIAEKIDRDRTTVQKALRSLMNCGLVEREKVTEKSGFKYIYRPIEFKEVKVKMEELLDEWYLSVKEWLKKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDP7</accession>
    <name>F6BDP7_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1069</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96608.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799209.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1069"/>
    <dbReference type="EnsemblBacteria" id="AEF96608">
        <property type="protein sequence ID" value="AEF96608"/>
        <property type="gene ID" value="Metig_1069"/>
    </dbReference>
    <dbReference type="GeneID" id="10643923"/>
    <dbReference type="KEGG" id="mig:Metig_1069"/>
    <dbReference type="HOGENOM" id="CLU_099320_1_0_2"/>
    <dbReference type="OMA" id="KNNMKVY"/>
    <dbReference type="OrthoDB" id="120839at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002798">
        <property type="entry name" value="SpoIIM-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01944">
        <property type="entry name" value="SpoIIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="43"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="71"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="147"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96608.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="178" mass="19713" checksum="608B38052FFD628A" modified="2011-07-27" version="1">MKLPSILTILIFSIGFISGITSINDFSKNYNGEELNQKLNIKFNFLTILLTNLKVILLMLAGAITFGLSTFINLIFNGFNVGILVGSTFLTDEPLKLITALILPHGIFEIPAMLISATAGFKIPYEVILYLLDKKEKPITEEDIKEFLKLAGISIILIIIAAFVEVYITPKIANYLLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDP8</accession>
    <name>F6BDP8_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Yip1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Metig_1070</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96609.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799210.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1070"/>
    <dbReference type="EnsemblBacteria" id="AEF96609">
        <property type="protein sequence ID" value="AEF96609"/>
        <property type="gene ID" value="Metig_1070"/>
    </dbReference>
    <dbReference type="GeneID" id="10643924"/>
    <dbReference type="KEGG" id="mig:Metig_1070"/>
    <dbReference type="HOGENOM" id="CLU_111437_0_0_2"/>
    <dbReference type="OMA" id="RTFEFTG"/>
    <dbReference type="OrthoDB" id="101247at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006977">
        <property type="entry name" value="Yip1_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04893">
        <property type="entry name" value="Yip1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="28"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="66"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="106"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="161"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="194"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="domain" description="Yip1" evidence="6">
        <location>
            <begin position="5"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEF96609.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="217" mass="24428" checksum="2BCB57FBBD540AA2" modified="2011-07-27" version="1">MNIKELILNPNNFFKNLADKDVSLKTPFLIVLMFSVLMCIYSYYTTSIMFKIFPPDMQNMMSIMRIISAVSTLVGGFIAWLLIAGVMHLISMAFKGEGSFKRTFEFTGYGFLPNLIALCITIPIGYYFLSNAHVPTLTMAQLQNPAVVKQVMSSIIPKPMVYTNLLIGIAVSLWNLGLWTYGIKYARNLELKKAFIVALIPTVLFGAYQLYSVAKFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDR1</accession>
    <name>F6BDR1_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Amino acid-binding ACT domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1083</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96622.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799223.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1083"/>
    <dbReference type="EnsemblBacteria" id="AEF96622">
        <property type="protein sequence ID" value="AEF96622"/>
        <property type="gene ID" value="Metig_1083"/>
    </dbReference>
    <dbReference type="GeneID" id="10643937"/>
    <dbReference type="KEGG" id="mig:Metig_1083"/>
    <dbReference type="HOGENOM" id="CLU_094842_1_0_2"/>
    <dbReference type="OMA" id="ERGTIPV"/>
    <dbReference type="OrthoDB" id="104907at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd04886">
        <property type="entry name" value="ACT_ThrD-II-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002912">
        <property type="entry name" value="ACT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044561">
        <property type="entry name" value="ACT_ThrD-II-like"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="SSF55021"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51671">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ACT" evidence="1">
        <location>
            <begin position="3"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96622.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="159" mass="18029" checksum="4817D0B5B9D573AF" modified="2011-07-27" version="1">MITLDLELKDIPGELIKALTPISKLGANIISVIHLREQKSNGRVPVKVVIDNVDNEKLKKIVDELEKHDVIVAKIGDDERKINLDVVVIGHVVDTDVRDTIDRINEIGLVVDLDLIMPDPYKESSARMRIVIDKEKLEELYNLLDKISKEKDLLFIKSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2020-06-17" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDS2</accession>
    <name>F6BDS2_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_1094</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96633.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96633">
        <property type="protein sequence ID" value="AEF96633"/>
        <property type="gene ID" value="Metig_1094"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_1094"/>
    <dbReference type="HOGENOM" id="CLU_3362563_0_0_2"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF96633.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="35" mass="4287" checksum="E4CD80991093C7E8" modified="2011-07-27" version="1">MEHNIIRIKDIIKFMELKPDSKGEVFELIVWIWKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDW6</accession>
    <name>F6BDW6_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transcription factor E</fullName>
            <shortName evidence="4">TFE</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">TFIIE subunit alpha homolog</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">Transcription initiation factor TFIIE</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">tfe</name>
        <name evidence="6" type="ordered locus">Metig_1138</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Transcription factor that plays a role in the activation of archaeal genes transcribed by RNA polymerase. Facilitates transcription initiation by enhancing TATA-box recognition by TATA-box-binding protein (Tbp), and transcription factor B (Tfb) and RNA polymerase recruitment. Not absolutely required for transcription in vitro, but particularly important in cases where Tbp or Tfb function is not optimal. It dynamically alters the nucleic acid-binding properties of RNA polymerases by stabilizing the initiation complex and destabilizing elongation complexes. Seems to translocate with the RNA polymerase following initiation and acts by binding to the non template strand of the transcription bubble in elongation complexes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Monomer. Interaction with RNA polymerase subunits RpoF and RpoE is necessary for Tfe stimulatory transcription activity. Able to interact with Tbp and RNA polymerase in the absence of DNA promoter. Interacts both with the preinitiation and elongation complexes.</text>
    </comment>
    <comment type="domain">
        <text evidence="4">The winged helix domain is involved in binding to DNA in the preinitiation complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the TFE family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96677.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799278.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1138"/>
    <dbReference type="EnsemblBacteria" id="AEF96677">
        <property type="protein sequence ID" value="AEF96677"/>
        <property type="gene ID" value="Metig_1138"/>
    </dbReference>
    <dbReference type="GeneID" id="10643996"/>
    <dbReference type="KEGG" id="mig:Metig_1138"/>
    <dbReference type="HOGENOM" id="CLU_100097_0_0_2"/>
    <dbReference type="OMA" id="DSGWLTY"/>
    <dbReference type="OrthoDB" id="75288at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation from RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01909">
        <property type="entry name" value="TFE_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016481">
        <property type="entry name" value="TF_E_archaea"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039997">
        <property type="entry name" value="TFE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017919">
        <property type="entry name" value="TFIIE/TFIIEa_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002853">
        <property type="entry name" value="TFIIE_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024550">
        <property type="entry name" value="TFIIEa/SarR/Rpc3_HTH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13097">
        <property type="entry name" value="PTHR13097"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02002">
        <property type="entry name" value="TFIIE_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006373">
        <property type="entry name" value="TF_E_archaea"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00531">
        <property type="entry name" value="TFIIE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00373">
        <property type="entry name" value="TIGR00373"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51344">
        <property type="entry name" value="HTH_TFE_IIE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0804">Transcription</keyword>
    <keyword evidence="1 4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH TFE/IIEalpha-type" evidence="5">
        <location>
            <begin position="13"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEF96677.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="184" mass="22295" checksum="E5ABD198508FB4CD" modified="2011-07-27" version="1">MKLSKEEIYKMFDNPLVQEVLFDIFDGEEEGFEILDVLLELGEATDDEIARKLNLKLNLVRKLLYKIYEARLVDYRREKDEDTNWYTYTWKPTIEKLPYVVKKRVNHILDDLKKRLEFEENNMFFYCPECNLRFVFDEAMETGFRCPRCDGILQEFDNREIIKSLKEQIAFFENEIKTNPLFSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDX7</accession>
    <name>F6BDX7_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">7-cyano-7-deazaguanine synthase</fullName>
            <ecNumber evidence="1">6.3.4.20</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">7-cyano-7-carbaguanine synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Archaeosine biosynthesis protein QueC</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">PreQ(0) synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">queC</name>
        <name evidence="2" type="ordered locus">Metig_1149</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the ATP-dependent conversion of 7-carboxy-7-deazaguanine (CDG) to 7-cyano-7-deazaguanine (preQ(0)).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>7-carboxy-7-deazaguanine + ATP + NH4(+) = 7-cyano-7-deazaguanine + ADP + H(+) + H2O + phosphate</text>
            <dbReference type="Rhea" id="RHEA:27982"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:45075"/>
            <dbReference type="ChEBI" id="CHEBI:61036"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.4.20"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Purine metabolism; 7-cyano-7-deazaguanine biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the QueC family.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.20" evidence="1"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96688.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799289.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1149"/>
    <dbReference type="EnsemblBacteria" id="AEF96688">
        <property type="protein sequence ID" value="AEF96688"/>
        <property type="gene ID" value="Metig_1149"/>
    </dbReference>
    <dbReference type="GeneID" id="10644007"/>
    <dbReference type="KEGG" id="mig:Metig_1149"/>
    <dbReference type="HOGENOM" id="CLU_081854_1_0_2"/>
    <dbReference type="OMA" id="CESCMRR"/>
    <dbReference type="OrthoDB" id="90765at2157"/>
    <dbReference type="UniPathway" id="UPA00391"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016879">
        <property type="term" value="F:ligase activity, forming carbon-nitrogen bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01995">
        <property type="entry name" value="ExsB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01633">
        <property type="entry name" value="QueC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018317">
        <property type="entry name" value="QueC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42914">
        <property type="entry name" value="PTHR42914"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06508">
        <property type="entry name" value="QueC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006293">
        <property type="entry name" value="ExsB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00364">
        <property type="entry name" value="TIGR00364"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0436">Ligase</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="7"/>
            <end position="17"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="196"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="207"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="210"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="213"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01633"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96688.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="233" mass="26064" checksum="8399F47832309AF7" modified="2011-07-27" version="1">MKAVCVLSGGLDSTVSCLIAKDLGYDLVNITFNYGQKAAKREVNSAKKISEILNAEHVVIDLPFIKKFGKSALLTEKEPPKLDIEELDMRFRTIQTMEEVWVPARNLILFSIASGFAESIDADRVFVGLSGEEATTFPDNTPEFVERLNKALEYGTLNQVKIEAPLCNKNKQEIVKIGHEIEKKLGVEVLKYSYSCYKDNGEDFLHCGVCESCARRKRAFLGAGVKDKTKYIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDY1</accession>
    <name>F6BDY1_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Adenosylmethionine-8-amino-7-oxononanoate aminotransferase</fullName>
            <ecNumber evidence="4">2.6.1.62</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">7,8-diamino-pelargonic acid aminotransferase</fullName>
            <shortName evidence="4">DAPA AT</shortName>
            <shortName evidence="4">DAPA aminotransferase</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">7,8-diaminononanoate synthase</fullName>
            <shortName evidence="4">DANS</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">Diaminopelargonic acid synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">bioA</name>
        <name evidence="6" type="ordered locus">Metig_1153</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Catalyzes the transfer of the alpha-amino group from S-adenosyl-L-methionine (SAM) to 7-keto-8-aminopelargonic acid (KAPA) to form 7,8-diaminopelargonic acid (DAPA). It is the only aminotransferase known to utilize SAM as an amino donor.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>(8S)-8-amino-7-oxononanoate + S-adenosyl-L-methionine = (7R,8S)-7,8-diammoniononanoate + S-adenosyl-4-methylsulfanyl-2-oxobutanoate</text>
            <dbReference type="Rhea" id="RHEA:16861"/>
            <dbReference type="ChEBI" id="CHEBI:16490"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:149468"/>
            <dbReference type="ChEBI" id="CHEBI:149469"/>
            <dbReference type="EC" id="2.6.1.62"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 4">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4">Cofactor biosynthesis; biotin biosynthesis; 7,8-diaminononanoate from 8-amino-7-oxononanoate (SAM route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. BioA subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.62" evidence="4"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96692.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799293.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1153"/>
    <dbReference type="EnsemblBacteria" id="AEF96692">
        <property type="protein sequence ID" value="AEF96692"/>
        <property type="gene ID" value="Metig_1153"/>
    </dbReference>
    <dbReference type="GeneID" id="10644011"/>
    <dbReference type="KEGG" id="mig:Metig_1153"/>
    <dbReference type="HOGENOM" id="CLU_016922_4_3_2"/>
    <dbReference type="OMA" id="VAVKMCL"/>
    <dbReference type="OrthoDB" id="20774at2157"/>
    <dbReference type="UniPathway" id="UPA00078">
        <property type="reaction ID" value="UER00160"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004015">
        <property type="term" value="F:adenosylmethionine-8-amino-7-oxononanoate transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009102">
        <property type="term" value="P:biotin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00610">
        <property type="entry name" value="OAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00834">
        <property type="entry name" value="BioA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005814">
        <property type="entry name" value="Aminotrans_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005815">
        <property type="entry name" value="BioA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00202">
        <property type="entry name" value="Aminotran_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000521">
        <property type="entry name" value="Transaminase_4ab_Lys_Orn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00508">
        <property type="entry name" value="bioA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00600">
        <property type="entry name" value="AA_TRANSFER_CLASS_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="4" id="KW-0093">Biotin biosynthesis</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 4 5" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4 6" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Pyridoxal phosphate binding" evidence="4">
        <location>
            <begin position="116"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Pyridoxal phosphate binding" evidence="4">
        <location>
            <begin position="331"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="149"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="262"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="295"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="330"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="425"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Participates in the substrate recognition with KAPA and in a stacking interaction with the adenine ring of SAM" evidence="4">
        <location>
            <position position="19"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="4">
        <location>
            <position position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEF96692.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="460" mass="51986" checksum="F4F6E4565496706E" modified="2011-07-27" version="1">MKNPELLEKWDKEYVWHPYTQMKEYVNSKNLIIERGEGNYLIDIYGNRYLDAVSSIWCNLFGHSRKEIIDAIKNQADKICHSTLLGCGNVPSILLAKKLVDITPKHLTKVFYSEDGAEAVEIAVKMAFEYYVLRGDKGRHKFISVKEGYHGDTVGAMSVGGSELFHGVFKPLLFKGYHADPPYCYRCKYYNFKDTDERNKKGCGMECLNEIVELIEKHSEEVFCVILEGGVMGSAGIIPFPDGYIEGVAKACKENDVIFILDEVATGFGRTGKMFFCDNEELKKLEKPDILCLGKGITGGYLPLAATLTTDEIYNMFLGDFGESKQFYHGHTYTGNQLLCSAALATLDIFEKENVIENIQPKIKLLHEELKKLKELEHVGDVRGRGFMVGIELVKNKETKEPYPYGYKAGYRVADKLLEKGIYMRPIGNVVILVPPLSITEEEIIYLCNSLYDAIKEADL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>F6BE16</accession>
    <name>F6BE16_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1189</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96727.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799326.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96727">
        <property type="protein sequence ID" value="AEF96727"/>
        <property type="gene ID" value="Metig_1189"/>
    </dbReference>
    <dbReference type="GeneID" id="10644049"/>
    <dbReference type="KEGG" id="mig:Metig_1189"/>
    <dbReference type="HOGENOM" id="CLU_058393_1_0_2"/>
    <dbReference type="OMA" id="KAWWHME"/>
    <dbReference type="OrthoDB" id="78248at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007160">
        <property type="entry name" value="DUF362"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04015">
        <property type="entry name" value="DUF362"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00037">
        <property type="entry name" value="Fer4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="300"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="331"/>
            <end position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96727.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="356" mass="41198" checksum="BC1B09C7A1814DDA" modified="2011-07-27" version="1">MEKVFYSIREDYDSLDNNILDLTFESCNFEKFDKILIKPNILGPYPPERAVTTHPKFLEWVIKYLQDNFNGKIIVGESSGYSTRKSFSVSGIEDICRKYDIKYIAFEKDEHIKTKILDREIPIPKTVVESDLIINLPKLKTHVLMKFTGAVKNLYGCIPGGLKPKLHGHFPKEDDFARLLVELYRFITKDKEIITIMDGIWGMEGNGPSNGKPKHSKIVIGSKNPVAVDLFASYYIGYKMDDILTNKLLKVDFEIIDADKNEKKSLNEIKPLKFKKPDTVYLNSFLPPQIVRIIFHLMTLKPKINKSRCIKCRICEKVCPVNAIKNLKIDRKKCINCYCCHEMCPYDAIELKRRFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>F6BE17</accession>
    <name>F6BE17_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Hydrolase, TatD family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_1190</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96728.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799327.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1190"/>
    <dbReference type="EnsemblBacteria" id="AEF96728">
        <property type="protein sequence ID" value="AEF96728"/>
        <property type="gene ID" value="Metig_1190"/>
    </dbReference>
    <dbReference type="GeneID" id="10644050"/>
    <dbReference type="KEGG" id="mig:Metig_1190"/>
    <dbReference type="HOGENOM" id="CLU_031506_5_2_2"/>
    <dbReference type="OMA" id="PNEAPRI"/>
    <dbReference type="OrthoDB" id="48998at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004536">
        <property type="term" value="F:deoxyribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01310">
        <property type="entry name" value="TatD_DNAse"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015991">
        <property type="entry name" value="Hydrolase_TatD-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001130">
        <property type="entry name" value="TatD-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01026">
        <property type="entry name" value="TatD_DNase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005902">
        <property type="entry name" value="DNase_TatD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="SSF51556"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00010">
        <property type="entry name" value="TIGR00010"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF96728.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="246" mass="28529" checksum="A15001503E4F449A" modified="2011-07-27" version="1">MRYIDAHCHIEDKAFNKNRDEIVKNAKENGVEIVTSGASLGGCRRALECKKKYNIYITLGFHPSNVRSDDKVVDEAYKLIEENEKEILAVGEIGLDIRVENKEKQEERFRRFINLAEELNKPIVIHAKGMERECFDIVNNKVISMYHCYSGDLDLAKEIINNGHYISISTLICFSEHHQNLVKNLNLENIVVETDSPYLSPIKGEKNEPKNVIKVIDKIWELKKDEYSKEEIIKIIYENTKRLYGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>F6BE23</accession>
    <name>F6BE23_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">4Fe-4S ferredoxin iron-sulfur binding domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1196</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96734.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799333.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1196"/>
    <dbReference type="EnsemblBacteria" id="AEF96734">
        <property type="protein sequence ID" value="AEF96734"/>
        <property type="gene ID" value="Metig_1196"/>
    </dbReference>
    <dbReference type="GeneID" id="10644056"/>
    <dbReference type="KEGG" id="mig:Metig_1196"/>
    <dbReference type="HOGENOM" id="CLU_693748_0_0_2"/>
    <dbReference type="OMA" id="KEDCIRC"/>
    <dbReference type="OrthoDB" id="93710at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00037">
        <property type="entry name" value="Fer4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12838">
        <property type="entry name" value="Fer4_7"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="11"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="22"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="60"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="94"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="126"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="156"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="194"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="231"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="261"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="300"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="338"/>
            <end position="367"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96734.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="397" mass="43666" checksum="A2901827369146CA" modified="2011-07-27" version="1">MIVINTEDCYLCKACERACPNEALKLNPLKVCMLCGNCVNACPNDALSLREINIDGKKIKQIGYNPSKCDLCGECVKVCPNNLKIEENKLKGFCVGCMKCIDACPDGFVGMEGVVEPNKKEIILPKEPIVVTDDCIGCGICANECPVNAIEIKDNKAVVDESKCIYCSICAQTCPWNAIFVAGRKVPKRDKTIKKFEVDEELCIGCGVCVEVCPRNLIKIENMVAIPPVGCPACGLCVNACPVNAITLEVEYEPPKPVSEEGLVWVEENCNYCGSCAMKCPTRAIRVINKRGMELPTKRKTEEKEKFRMCVRCGACTVACPTGALKLGKITHNGKEYNRIEFKPHLCNKCGKCVEICPYNVLELTDNNEMPLKGFCVMCLKCVEACEKIKRNALALK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEA1</accession>
    <name>F6BEA1_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="9 17">Tetrahydromethanopterin S-methyltransferase subunit B</fullName>
            <ecNumber evidence="8 17">2.1.1.86</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16 17">N5-methyltetrahydromethanopterin--coenzyme M methyltransferase subunit B</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="primary">mtrB</name>
        <name evidence="18" type="ordered locus">Metig_1241</name>
    </gene>
    <organism evidence="19">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 17">Part of a complex that catalyzes the formation of methyl-coenzyme M and tetrahydromethanopterin from coenzyme M and methyl-tetrahydromethanopterin. This is an energy-conserving, sodium-ion translocating step.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 17">
            <text>5-methyl-5,6,7,8-tetrahydromethanopterin + coenzyme M + 2 Na(+)(in) = 5,6,7,8-tetrahydromethanopterin + methyl-coenzyme M + 2 Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:53492"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:58103"/>
            <dbReference type="ChEBI" id="CHEBI:58116"/>
            <dbReference type="ChEBI" id="CHEBI:58286"/>
            <dbReference type="ChEBI" id="CHEBI:58319"/>
            <dbReference type="EC" id="2.1.1.86"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="5 17">One-carbon metabolism; methanogenesis from CO(2); methyl-coenzyme M from 5,10-methylene-5,6,7,8-tetrahydromethanopterin: step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7 17">The complex is composed of 8 subunits; MtrA, MtrB, MtrC, MtrD, MtrE, MtrF, MtrG and MtrH.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3 17">Cell membrane</location>
            <topology evidence="3 17">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Membrane</location>
            <topology evidence="4">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6 17">Belongs to the MtrB family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.86" evidence="8 17"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96778.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799377.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1241"/>
    <dbReference type="EnsemblBacteria" id="AEF96778">
        <property type="protein sequence ID" value="AEF96778"/>
        <property type="gene ID" value="Metig_1241"/>
    </dbReference>
    <dbReference type="GeneID" id="10644104"/>
    <dbReference type="KEGG" id="mig:Metig_1241"/>
    <dbReference type="HOGENOM" id="CLU_171544_0_0_2"/>
    <dbReference type="OMA" id="TNMVYGF"/>
    <dbReference type="OrthoDB" id="114178at2157"/>
    <dbReference type="UniPathway" id="UPA00640">
        <property type="reaction ID" value="UER00698"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030269">
        <property type="term" value="F:tetrahydromethanopterin S-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019386">
        <property type="term" value="P:methanogenesis, from carbon dioxide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01094">
        <property type="entry name" value="MtrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008690">
        <property type="entry name" value="MtrB_MeTrfase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05440">
        <property type="entry name" value="MtrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005518">
        <property type="entry name" value="MtrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR04166">
        <property type="entry name" value="methano_MtrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="17" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="15 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-0484">Methanogenesis</keyword>
    <keyword evidence="11 17 18" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="10 17" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12 17 18" id="KW-0808">Transferase</keyword>
    <keyword evidence="13 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14 17" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="81"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00010027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00011616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00012514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00015127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00029818"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="AEF96778.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="108" mass="12097" checksum="C32D03EB0893B28E" modified="2011-07-27" version="1">MEVVKICPEIDVVMEVNSGLVAEMRKDVLVVDLTHVEEEVKKLERLATALKNSLDPRNPPLNTFDNRDGVYKISGLFKGMFFGFWITLAILVLVVILVIKTNLSLIGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEA6</accession>
    <name>F6BEA6_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="6">DEAD/H associated domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Metig_1246</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96783.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1246"/>
    <dbReference type="EnsemblBacteria" id="AEF96783">
        <property type="protein sequence ID" value="AEF96783"/>
        <property type="gene ID" value="Metig_1246"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_1246"/>
    <dbReference type="HOGENOM" id="CLU_008427_0_0_2"/>
    <dbReference type="OMA" id="GFWMADG"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016818">
        <property type="term" value="F:hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016539">
        <property type="term" value="P:intein-mediated protein splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.28.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013701">
        <property type="entry name" value="DEAD/DEAH_assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003586">
        <property type="entry name" value="Hint_dom_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003587">
        <property type="entry name" value="Hint_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036844">
        <property type="entry name" value="Hint_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027434">
        <property type="entry name" value="Homing_endonucl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006142">
        <property type="entry name" value="INTEIN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004042">
        <property type="entry name" value="Intein_endonuc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006141">
        <property type="entry name" value="Intein_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004860">
        <property type="entry name" value="LAGLIDADG_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045628">
        <property type="entry name" value="Lhr_WH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08494">
        <property type="entry name" value="DEAD_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14528">
        <property type="entry name" value="LAGLIDADG_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19306">
        <property type="entry name" value="Lhr_WH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00379">
        <property type="entry name" value="INTEIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00305">
        <property type="entry name" value="HintC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00306">
        <property type="entry name" value="HintN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51294">
        <property type="entry name" value="SSF51294"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55608">
        <property type="entry name" value="SSF55608"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01445">
        <property type="entry name" value="intein_Nterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50819">
        <property type="entry name" value="INTEIN_ENDONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50817">
        <property type="entry name" value="INTEIN_N_TER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0068">Autocatalytic cleavage</keyword>
    <keyword evidence="2" id="KW-0651">Protein splicing</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="4">
        <location>
            <begin position="49"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="domain" description="DOD-type homing endonuclease" evidence="3">
        <location>
            <begin position="547"/>
            <end position="684"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="5">
        <location>
            <begin position="772"/>
            <end position="947"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEF96783.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="1374" mass="158854" checksum="0A53560C6F8940AA" modified="2011-07-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEC0</accession>
    <name>F6BEC0_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Dimethylallyltranstransferase</fullName>
            <ecNumber evidence="4">2.5.1.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metig_1260</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the FPP/GGPP synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.1" evidence="4"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96797.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799395.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1260"/>
    <dbReference type="EnsemblBacteria" id="AEF96797">
        <property type="protein sequence ID" value="AEF96797"/>
        <property type="gene ID" value="Metig_1260"/>
    </dbReference>
    <dbReference type="GeneID" id="10644123"/>
    <dbReference type="KEGG" id="mig:Metig_1260"/>
    <dbReference type="HOGENOM" id="CLU_014015_2_1_2"/>
    <dbReference type="OMA" id="CEGQALD"/>
    <dbReference type="OrthoDB" id="55053at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004161">
        <property type="term" value="F:dimethylallyltranstransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008299">
        <property type="term" value="P:isoprenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00685">
        <property type="entry name" value="Trans_IPPS_HT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.600.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008949">
        <property type="entry name" value="Isoprenoid_synthase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000092">
        <property type="entry name" value="Polyprenyl_synt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033749">
        <property type="entry name" value="Polyprenyl_synt_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00348">
        <property type="entry name" value="polyprenyl_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48576">
        <property type="entry name" value="SSF48576"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00723">
        <property type="entry name" value="POLYPRENYL_SYNTHASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00444">
        <property type="entry name" value="POLYPRENYL_SYNTHASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="272"/>
            <end position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU004466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEF96797.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="320" mass="36353" checksum="9012DACA0BE3B369" modified="2011-07-27" version="1">MSNELFDKGILGKIEEELKAYVGNEGKLYDASKHLLFAGGKRIRPYLAVLTYMLKKDNIEEVLSPAVAVELIHNYTLIHDDIMDNDDERRGKPTVHVVYGVPMAILAGDLLYAKAFEAITKIKDSKKAYEVLKVLARACVEVCEGQAMDMEFENYFPSMDEYFEMISKKTGALIVASVEIGAIMADCNEEERKALIEYAKRIGMTFQIQDDVLDLIGDKKTIGKPVGSDIREGKKTLMVIHAMETLDEDKKKRLLEILGNENATDEEVKEAIEILSDSIEYAKELMKKATEEAKEYLKIFDKEKRKKLEDIADFIINRIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEC2</accession>
    <name>F6BEC2_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Isopentenyl-diphosphate delta-isomerase</fullName>
            <shortName evidence="7">IPP isomerase</shortName>
            <ecNumber evidence="7">5.3.3.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Isopentenyl diphosphate:dimethylallyl diphosphate isomerase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Isopentenyl pyrophosphate isomerase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Type 2 isopentenyl diphosphate isomerase</fullName>
            <shortName evidence="7">IDI-2</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">fni</name>
        <name evidence="9" type="ordered locus">Metig_1262</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Involved in the biosynthesis of isoprenoids. Catalyzes the 1,3-allylic rearrangement of the homoallylic substrate isopentenyl (IPP) to its allylic isomer, dimethylallyl diphosphate (DMAPP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>isopentenyl diphosphate = dimethylallyl diphosphate</text>
            <dbReference type="Rhea" id="RHEA:23284"/>
            <dbReference type="ChEBI" id="CHEBI:57623"/>
            <dbReference type="ChEBI" id="CHEBI:128769"/>
            <dbReference type="EC" id="5.3.3.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 7">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>NADPH</name>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="6 7">Homooctamer. Dimer of tetramers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the IPP isomerase type 2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="5.3.3.2" evidence="7"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96799.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799397.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1262"/>
    <dbReference type="EnsemblBacteria" id="AEF96799">
        <property type="protein sequence ID" value="AEF96799"/>
        <property type="gene ID" value="Metig_1262"/>
    </dbReference>
    <dbReference type="GeneID" id="10644125"/>
    <dbReference type="KEGG" id="mig:Metig_1262"/>
    <dbReference type="HOGENOM" id="CLU_065515_1_0_2"/>
    <dbReference type="OMA" id="WDWGIPT"/>
    <dbReference type="OrthoDB" id="42330at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004452">
        <property type="term" value="F:isopentenyl-diphosphate delta-isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070402">
        <property type="term" value="F:NADPH binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008299">
        <property type="term" value="P:isoprenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02811">
        <property type="entry name" value="IDI-2_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00354">
        <property type="entry name" value="Idi_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000262">
        <property type="entry name" value="FMN-dep_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011179">
        <property type="entry name" value="IPdP_isomerase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43665">
        <property type="entry name" value="PTHR43665"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01070">
        <property type="entry name" value="FMN_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003314">
        <property type="entry name" value="IPP_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02151">
        <property type="entry name" value="IPP_isom_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="7" id="KW-0288">FMN</keyword>
    <keyword evidence="5 7 9" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4 7" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 7" id="KW-0521">NADP</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FMN_dh" evidence="8">
        <location>
            <begin position="191"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="7">
        <location>
            <begin position="9"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="7">
        <location>
            <begin position="97"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="67"/>
            <end position="69"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="97"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="125"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="161"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="162"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="197"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="227"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="276"/>
            <end position="278"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="297"/>
            <end position="298"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AEF96799.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="359" mass="39461" checksum="CBC0DD457E6D68A3" modified="2011-07-27" version="1">MVNHDIEARKLEHILVCNYCDVEYKKGTLLDNVELIHRGIPECDLEDIDTSVKLFGKTLDAPIIVAGMTGGHSKAKEINKNIAIAIEDLNLGMGLGSQRAAIANEELIDTYSIVRKYTDSLVIGNLGAVNFIKDGWDERIIDKAVEMINADAIAIHFNPLQEAIQPEGDVNFKGTDILRDIISSYKNNYKNIPFIAKQVGEGFSKEDAKIINDLGFDAIDVGGSGGTSWAAVELYRIKDEEFKELAENFINWGIPTAASIFEVRSVFDKTLIATGGIRNGIDIAKSIAIGADCCGIALPILKAALKGPEEIVKVLERLIKELKIAMFLTGCDSIEKLKKAPYIVKGELKDWISQRLKKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEC8</accession>
    <name>F6BEC8_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">8-amino-7-ketopelargonate synthase</fullName>
            <ecNumber evidence="7">2.3.1.47</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Metig_1268</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the decarboxylative condensation of pimeloyl-[acyl-carrier protein] and L-alanine to produce 8-amino-7-oxononanoate (AON), [acyl-carrier protein], and carbon dioxide.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5 7">
            <text>6-carboxyhexanoyl-[ACP] + H(+) + L-alanine = (8S)-8-amino-7-oxononanoate + CO2 + holo-[ACP]</text>
            <dbReference type="Rhea" id="RHEA:42288"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="Rhea" id="RHEA-COMP:9955"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57972"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:78846"/>
            <dbReference type="ChEBI" id="CHEBI:149468"/>
            <dbReference type="EC" id="2.3.1.47"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 6 7">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2 7">Cofactor biosynthesis; biotin biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 7">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family. BioF subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.47" evidence="7"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96805.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1268"/>
    <dbReference type="EnsemblBacteria" id="AEF96805">
        <property type="protein sequence ID" value="AEF96805"/>
        <property type="gene ID" value="Metig_1268"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_1268"/>
    <dbReference type="HOGENOM" id="CLU_015846_11_3_2"/>
    <dbReference type="OMA" id="MDTHGFG"/>
    <dbReference type="OrthoDB" id="39668at2157"/>
    <dbReference type="UniPathway" id="UPA00078"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008710">
        <property type="term" value="F:8-amino-7-oxononanoate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009102">
        <property type="term" value="P:biotin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001917">
        <property type="entry name" value="Aminotrans_II_pyridoxalP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004723">
        <property type="entry name" value="AONS_Archaea/Proteobacteria"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00858">
        <property type="entry name" value="bioF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00599">
        <property type="entry name" value="AA_TRANSFER_CLASS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="4" id="KW-0093">Biotin biosynthesis</keyword>
    <keyword evidence="6 7" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotran_1_2" evidence="8">
        <location>
            <begin position="28"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="6">
        <location>
            <position position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR604723-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AEF96805.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="390" mass="44014" checksum="2E9CAC013CBFD4A9" modified="2011-07-27" version="1">MFREKLKREIEIIKSNGLYRFLREKRDNILDFSSNDYLCLSKHPEVIEAVKEGLKYGVGSTGSRLTSGNINHQRLEEKIAEFKETERALVYSSGYATNVGVISALCKKGDLILSDKLNHASIIDGCRLSKADVLIYNHCDVEHLANLIEENWKKYNNLFIITDGVFSMDGDVAPLRDLKKIADEFNAILIIDDAHGTGVLGDGKGTLKHFNLKPSDNIIQIGTLSKAIGGLGGFVCGIDEVVEYLINTSRSFIYSTALPPHVVEGCIKAFEIIERGDVVKELQKNIKIANGIFKKNGFIKENNLTPIYPFIFKEKTMLIAEHLIKNNIFCVGIRYPTVPKGLERIRVSINVSHKKEDFELLCEKIKDIYQNHLNQTTNNPYPLYSRLFSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEE8</accession>
    <name>F6BEE8_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Methyltransferase type 11</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0048</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95609.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798218.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0048"/>
    <dbReference type="EnsemblBacteria" id="AEF95609">
        <property type="protein sequence ID" value="AEF95609"/>
        <property type="gene ID" value="Metig_0048"/>
    </dbReference>
    <dbReference type="GeneID" id="10642883"/>
    <dbReference type="KEGG" id="mig:Metig_0048"/>
    <dbReference type="HOGENOM" id="CLU_084119_0_0_2"/>
    <dbReference type="OMA" id="CINVLEH"/>
    <dbReference type="OrthoDB" id="45753at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013216">
        <property type="entry name" value="Methyltransf_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08241">
        <property type="entry name" value="Methyltransf_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransf_11" evidence="1">
        <location>
            <begin position="52"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF95609.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="229" mass="27145" checksum="FA4C71B6EBF72A2F" modified="2011-07-27" version="1">MKVKDFYENWEIGCIPKYAKLLMNFEEELIFDIISNLDEFNRIKNKKEALILDCGCGFGSFYNLTKDLNTIYLDISFNLLKKFNIKTNKICGDILNLPFKDNSFDLILCINVLEHVDYKKALSEMRRVLKKEGTCIIVVVNKNSIINEELFTDWKIYHQPLDIKDFESIEGFYIAYWKTFYFVHPIFKIFPTKILGKFLKFFKKVDNKLANTKMFKNKGQFLICVLKVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEI0</accession>
    <name>F6BEI0_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ammonium transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Metig_0080</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cell membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the ammonia transporter channel (TC 1.A.11.2) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95641.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798250.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0080"/>
    <dbReference type="EnsemblBacteria" id="AEF95641">
        <property type="protein sequence ID" value="AEF95641"/>
        <property type="gene ID" value="Metig_0080"/>
    </dbReference>
    <dbReference type="GeneID" id="10642915"/>
    <dbReference type="KEGG" id="mig:Metig_0080"/>
    <dbReference type="HOGENOM" id="CLU_000445_33_1_2"/>
    <dbReference type="OMA" id="IWLSIMS"/>
    <dbReference type="OrthoDB" id="66605at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008519">
        <property type="term" value="F:ammonium transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3430.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029020">
        <property type="entry name" value="Ammonium/urea_transptr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001905">
        <property type="entry name" value="Ammonium_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018047">
        <property type="entry name" value="Ammonium_transpt_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024041">
        <property type="entry name" value="NH4_transpt_AmtB-like_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00909">
        <property type="entry name" value="Ammonium_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00836">
        <property type="entry name" value="amt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01219">
        <property type="entry name" value="AMMONIUM_TRANSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0924">Ammonia transport</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="29"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="70"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="115"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="141"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="173"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="207"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="242"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="269"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="293"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="325"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="359"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="domain" description="Ammonium_transp" evidence="9">
        <location>
            <begin position="30"/>
            <end position="408"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU362002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AEF95641.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="416" mass="43139" checksum="EC720907A3884F78" modified="2011-07-27" version="1">MATADLFQNAADINSIVQALTAMANASDVFFLVVMGVLVFMMQWGFAMLEGGQVRKKNVNNVMMKNMVDWFIGCVSWLFIGGVLCASINPADFISWWQQIFGTNWANNGIDLANWFFGLVFCATAATIVSGGVAERIKFSAYVLISIIITAFLYPFFVYLGPWGAGIINWHDYAGSLIVHGLGGFLALGAIAAVGPRVGRFKDGKPVPILGHNIPMAVFGALALAIGWYGFNVGSSLALGDISGLVCATTTLAMAGGGIGALIASKKDVLFTANGIVAGLVAICSGTDVVSPIGGLLIGLIAGLQVPIVFRLLENAGLDDVCGVVPVHGTAGVVGAILAGILGMEALGGAGGVSLVEQIIASVVTIIYGTALGFIIAKIAGVICGGLRVSEEEEFKGLDLAEHKMPAYPEEETSVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEK8</accession>
    <name>F6BEK8_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">4Fe-4S ferredoxin iron-sulfur binding domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0108</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95669.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798278.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0108"/>
    <dbReference type="EnsemblBacteria" id="AEF95669">
        <property type="protein sequence ID" value="AEF95669"/>
        <property type="gene ID" value="Metig_0108"/>
    </dbReference>
    <dbReference type="GeneID" id="10642943"/>
    <dbReference type="KEGG" id="mig:Metig_0108"/>
    <dbReference type="HOGENOM" id="CLU_139698_5_5_2"/>
    <dbReference type="OMA" id="CYDVCPK"/>
    <dbReference type="OrthoDB" id="111853at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="3"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="36"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF95669.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="69" mass="7867" checksum="E05D27E50E179C7E" modified="2011-07-27" version="1">MAVEIIVDREKCIGCGKCYDVCPKGPLIWKKDEDGKYYAYSVEYCHNCKFCAGRCPTNAIQVLVKKNEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEL2</accession>
    <name>F6BEL2_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Flavodoxin/nitric oxide synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0112</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95673.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798282.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0112"/>
    <dbReference type="EnsemblBacteria" id="AEF95673">
        <property type="protein sequence ID" value="AEF95673"/>
        <property type="gene ID" value="Metig_0112"/>
    </dbReference>
    <dbReference type="GeneID" id="10642947"/>
    <dbReference type="KEGG" id="mig:Metig_0112"/>
    <dbReference type="HOGENOM" id="CLU_017490_0_0_2"/>
    <dbReference type="OMA" id="PEAPIYC"/>
    <dbReference type="OrthoDB" id="72715at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008254">
        <property type="entry name" value="Flavodoxin/NO_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029039">
        <property type="entry name" value="Flavoprotein-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016440">
        <property type="entry name" value="Rubredoxin-O_OxRdtase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00258">
        <property type="entry name" value="Flavodoxin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005243">
        <property type="entry name" value="ROO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52218">
        <property type="entry name" value="SSF52218"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="SSF56281"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50902">
        <property type="entry name" value="FLAVODOXIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Flavodoxin-like" evidence="1">
        <location>
            <begin position="260"/>
            <end position="401"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF95673.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="407" mass="46408" checksum="16E9D423ACDE40FA" modified="2011-07-27" version="1">MKADAVKIADGVYWVGVLDWDIRKYHGYTLKGTTYNAYLVFGEEKVALIDNTYPGTSAQMWGRIKDAFEKEGREFKIDVIIQNHVEKDHSGALPEIHKKFPEAPIYCTEVAVEGLKKHYPSLKDAPFKVVKSLDKVDLGGKTLVFLEAPLLHWPDSMFTFYAEEGILFSNDAFGQHLCFTKRFDHEIPEYVLMDATKKFYANLITPLSKLVLKKFEEVKELGLLEKIKMIAPSHGQIWTDPMKVINAYADWATGKCKDKVTIVYDTMHYSTQKMAHAFAEGLISGGVDVVMYYLHEDERSEIVKDILDSKAVLFGIPTIYDEPYPSMGDLIYYLRGLRFNRTGRKRLALVFGSMGGEGGAVKKLAEELKECGFEVLDEYELYYVPTEDELEKCYNMGKRLAAKIKEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEL3</accession>
    <name>F6BEL3_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">4-vinyl reductase 4VR</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0113</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95674.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798283.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0113"/>
    <dbReference type="EnsemblBacteria" id="AEF95674">
        <property type="protein sequence ID" value="AEF95674"/>
        <property type="gene ID" value="Metig_0113"/>
    </dbReference>
    <dbReference type="GeneID" id="10642948"/>
    <dbReference type="KEGG" id="mig:Metig_0113"/>
    <dbReference type="HOGENOM" id="CLU_1551833_0_0_2"/>
    <dbReference type="OMA" id="ICYFDAG"/>
    <dbReference type="OrthoDB" id="114615at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024096">
        <property type="entry name" value="NO_sig/Golgi_transp_ligand-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004096">
        <property type="entry name" value="V4R"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02830">
        <property type="entry name" value="V4R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00989">
        <property type="entry name" value="V4R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111126">
        <property type="entry name" value="SSF111126"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="V4R" evidence="1">
        <location>
            <begin position="129"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF95674.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="193" mass="22782" checksum="AD5E0E23D4ACCC74" modified="2011-07-27" version="1">MHIQLKLNREMSEIFREILVDKETDDLPDDDELINEVVEHLKKGFDKEKMTFDERKYTDRKIKEYIPLELFRVMAYIVIKKIKERGSEITLYEIGYEFGKFLKPKNIRELKTFFRKNNLGILEIESRKPFILKVRECAMCDSLEVNEPICYFDAGLLAGALECILNKTVVVDEIKCMGQGYDACYFKIEIVKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEN4</accession>
    <name>F6BEN4_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">5-amino-6-(D-ribitylamino)uracil--L-tyrosine 4-hydroxyphenyl transferase</fullName>
            <ecNumber evidence="3 9">2.5.1.147</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">FO synthase subunit 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">cofH</name>
        <name evidence="11" type="ordered locus">Metig_1294</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the radical-mediated synthesis of 5-amino-5-(4-hydroxybenzyl)-6-(D-ribitylimino)-5,6-dihydrouracil from 5-amino-6-(D-ribitylamino)uracil and L-tyrosine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>5-amino-6-(D-ribitylamino)uracil + L-tyrosine + S-adenosyl-L-methionine = 2-iminoacetate + 5'-deoxyadenosine + 5-amino-5-(4-hydroxybenzyl)-6-(D-ribitylimino)-5,6-dihydrouracil + H(+) + L-methionine</text>
            <dbReference type="Rhea" id="RHEA:55200"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15934"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:58315"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:77846"/>
            <dbReference type="ChEBI" id="CHEBI:85936"/>
            <dbReference type="EC" id="2.5.1.147"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="9">Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 9">Cofactor biosynthesis; coenzyme F0 biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">The FO synthase complex consists of two subunits, CofG and CofH.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the radical SAM superfamily. CofH family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.147" evidence="3 9"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96831.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799428.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1294"/>
    <dbReference type="EnsemblBacteria" id="AEF96831">
        <property type="protein sequence ID" value="AEF96831"/>
        <property type="gene ID" value="Metig_1294"/>
    </dbReference>
    <dbReference type="GeneID" id="10644157"/>
    <dbReference type="KEGG" id="mig:Metig_1294"/>
    <dbReference type="HOGENOM" id="CLU_040406_1_0_2"/>
    <dbReference type="OMA" id="ATMMFGS"/>
    <dbReference type="OrthoDB" id="26917at2157"/>
    <dbReference type="UniPathway" id="UPA00072"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044689">
        <property type="term" value="F:7,8-didemethyl-8-hydroxy-5-deazariboflavin synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01612">
        <property type="entry name" value="FO_synth_sub2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045567">
        <property type="entry name" value="CofH/MnqC-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019940">
        <property type="entry name" value="CofH_family"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaB/NifB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034405">
        <property type="entry name" value="F420"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020050">
        <property type="entry name" value="FO_synthase_su2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43076">
        <property type="entry name" value="PTHR43076"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19288">
        <property type="entry name" value="CofH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00293">
        <property type="entry name" value="((2_3_4_5-tetrahydroxypentyl)a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00343">
        <property type="entry name" value="aminofutalosine_synthase_(mqnE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03551">
        <property type="entry name" value="F420_cofH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00423">
        <property type="entry name" value="TIGR00423"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="7 9" id="KW-0408">Iron</keyword>
    <keyword evidence="8 9" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="6 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Radical SAM core" evidence="10">
        <location>
            <begin position="45"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="59"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="63"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="66"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004712"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AEF96831.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="359" mass="41097" checksum="87F1404A99125E26" modified="2011-07-27" version="1">MDLEKFREKEISKRECLELFENDELFFDVLRFADELRRETVGDIVTYVVNRNINFTNICIGDCKFCAFRVNENDKNAYFLDIDEIARRALEAKKIGATEVCIQGGLHPKVDTYFQIEILKKVHEITKPYGDIHIHAFSPMEVYFAAENAGLDVKEALKMLKENGLNSMPGTAAEILDDEVRRELCPHKISTKQWVDIIKTAHKLGIPTTSTMMYGHIDEYKHWVKHLFLLKEIQKETNGFTEFVPLSFMHKYAPIYKEGRAKAGATGIEDLKVYAVSRIVLNDYIKNIQVSWVKLGQKMAQIALKCGGNDVGGTLMEENISRAAGAEYGVSMSVEEIRNMIERIGRIPKERTTLYKILE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>F6BER4</accession>
    <name>F6BER4_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Energy-converting hydrogenase B, subunit P</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_1324</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96861.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799458.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1324"/>
    <dbReference type="EnsemblBacteria" id="AEF96861">
        <property type="protein sequence ID" value="AEF96861"/>
        <property type="gene ID" value="Metig_1324"/>
    </dbReference>
    <dbReference type="GeneID" id="10644187"/>
    <dbReference type="KEGG" id="mig:Metig_1324"/>
    <dbReference type="HOGENOM" id="CLU_162437_0_0_2"/>
    <dbReference type="OMA" id="KPFPYRN"/>
    <dbReference type="OrthoDB" id="113300at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019597">
        <property type="entry name" value="Energy-convert_hydgase-B_suP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10622">
        <property type="entry name" value="Ehbp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF96861.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="95" mass="10876" checksum="A1E0A4223B4771DC" modified="2011-07-27" version="1">MPKMVLLPKLTMALGGYIRETVMPYSEDEAKPFPYRNVIVGNPTDEPIKIDVPAYDEDWIERHRKLGLIVVPVTENDDFVGLFKMVEEKVKRGGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>F6BES0</accession>
    <name>F6BES0_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_1330</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96867.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799464.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1330"/>
    <dbReference type="EnsemblBacteria" id="AEF96867">
        <property type="protein sequence ID" value="AEF96867"/>
        <property type="gene ID" value="Metig_1330"/>
    </dbReference>
    <dbReference type="GeneID" id="10644193"/>
    <dbReference type="KEGG" id="mig:Metig_1330"/>
    <dbReference type="HOGENOM" id="CLU_100102_0_0_2"/>
    <dbReference type="OMA" id="NTCSPYL"/>
    <dbReference type="OrthoDB" id="85931at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007386">
        <property type="entry name" value="DUF447"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012349">
        <property type="entry name" value="Split_barrel_FMN-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016733">
        <property type="entry name" value="UCP018747"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04289">
        <property type="entry name" value="DUF447"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018747">
        <property type="entry name" value="UCP018747"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF96867.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="206" mass="24053" checksum="678901E948677290" modified="2011-07-27" version="1">MIHEVVITSGKKNKAPIGIYFRAKSEGFCNSTLRSDKEKEVVLHLFEGSHTYENLKNDDYFVVNVCHPIDIAEAVLDDEDDYGYLDWNGKELPYLKNAYKIFVVKINKRKFITKRDNFGESKLMVVNGEIVFEKELNNIITPYNRADGLLVEMAVIYSRLNNEKIIMKNDDREAMKKDMEKYFKIIKKVGSKKHVDLAKRFMELIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEU0</accession>
    <name>F6BEU0_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF357 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1352</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96887.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799484.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1352"/>
    <dbReference type="EnsemblBacteria" id="AEF96887">
        <property type="protein sequence ID" value="AEF96887"/>
        <property type="gene ID" value="Metig_1352"/>
    </dbReference>
    <dbReference type="GeneID" id="10644215"/>
    <dbReference type="KEGG" id="mig:Metig_1352"/>
    <dbReference type="HOGENOM" id="CLU_180506_0_0_2"/>
    <dbReference type="OMA" id="GHLFTVE"/>
    <dbReference type="OrthoDB" id="126162at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036809">
        <property type="entry name" value="AF1782-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023140">
        <property type="entry name" value="DUF357"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04010">
        <property type="entry name" value="DUF357"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158372">
        <property type="entry name" value="SSF158372"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF357" evidence="1">
        <location>
            <begin position="21"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96887.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="106" mass="12123" checksum="A3D579D2EB82D79C" modified="2011-07-27" version="1">MSEKNEKNIKNIITDEKLNDYLKRTEEAIQIIKKGLPPKRSLLHDVAEDFLLMIESYYKDAKAFIEVGDYVNAFASLNYAYGWIDAGARLGIFDVGDDDVRFTLAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEW4</accession>
    <name>F6BEW4_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0140</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95700.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798309.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0140"/>
    <dbReference type="EnsemblBacteria" id="AEF95700">
        <property type="protein sequence ID" value="AEF95700"/>
        <property type="gene ID" value="Metig_0140"/>
    </dbReference>
    <dbReference type="GeneID" id="10642975"/>
    <dbReference type="KEGG" id="mig:Metig_0140"/>
    <dbReference type="HOGENOM" id="CLU_046841_0_1_2"/>
    <dbReference type="OMA" id="FGYLIMM"/>
    <dbReference type="OrthoDB" id="29920at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="25"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="181"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="227"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="264"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="299"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="354"/>
            <end position="376"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF95700.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="401" mass="45086" checksum="16AB80AEFBC7D0D2" modified="2011-07-27" version="1">MKINSSKIKTIAKHEILSTIKRKQFIIGTVGVPLVMIIIAIIGLYFSSDIGNLKIGYIDYFGVNIPDKIVKYNSIQQKNITLYFIEYHNIEEGKNDVLNGTIDILAIIPKDYLDTGKIIIYSTTKSINPTIAETLRDLLLETLLKDKVDNKTYNRVKSPLNLEIYSISEKGEEKESIFSQILPIGFVMILYIAITTVSGLIVGNTIEEKEHRIMEVLLSFTNAENIMIGKILGISVVGLIQISIWLMFALPVVIVYAIKVSTFLVAMAVVFFILAYLFYTSLLCGVASLYTNLKDASQIIAPIVIIQTIPFMMLNLILTNPNHYVVKILSYLPFTAPQVMLMRLSITNVEIWELVLSLAIMVLSTIASFIISIKLFKIGTLIYEENMSLKKVLKILRGNRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2020-08-12" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEX7</accession>
    <name>F6BEX7_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0155</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95713.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF95713">
        <property type="protein sequence ID" value="AEF95713"/>
        <property type="gene ID" value="Metig_0155"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0155"/>
    <dbReference type="HOGENOM" id="CLU_202278_0_0_2"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="15"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF95713.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="48" mass="5766" checksum="203C13DE9EF5BBB2" modified="2011-07-27" version="1">MNRFWTNVKGFKKKYVFCMLSVITAQILAIYNLKTLNYPLIRIKDVRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEX8</accession>
    <name>F6BEX8_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Rhodanese-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0156</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95714.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798323.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0156"/>
    <dbReference type="EnsemblBacteria" id="AEF95714">
        <property type="protein sequence ID" value="AEF95714"/>
        <property type="gene ID" value="Metig_0156"/>
    </dbReference>
    <dbReference type="GeneID" id="10642991"/>
    <dbReference type="KEGG" id="mig:Metig_0156"/>
    <dbReference type="HOGENOM" id="CLU_1237913_0_0_2"/>
    <dbReference type="OMA" id="FEPVNIK"/>
    <dbReference type="OrthoDB" id="64335at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.250.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030818">
        <property type="entry name" value="Arch_SelU_Nterm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036873">
        <property type="entry name" value="Rhodanese-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00581">
        <property type="entry name" value="Rhodanese"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00450">
        <property type="entry name" value="RHOD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52821">
        <property type="entry name" value="SSF52821"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR04568">
        <property type="entry name" value="arch_SelU_Nterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rhodanese" evidence="1">
        <location>
            <begin position="104"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF95714.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="220" mass="25265" checksum="E72A8113799211AC" modified="2011-07-27" version="1">MDEEILARLLTFKDDVVLSIILNDGRKMITDGKKILAGKVSGELASFILNKSKEVLENKKIEVVEFNNYKIYFEPIDIKNYLNSVGKELTDDLITVSDLEELMKKDEVIIVDVRSPREYKERTIPNAINIPLFLDEEHELIGKIYKHEGKDKAMEVAAKIIEEGIRRIMREVTKLDRNKTIVVFCARGGMRSQSIALILKLLGFRVKRLIGGFKAYKHIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEZ4</accession>
    <name>F6BEZ4_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Putative thymidylate synthase</fullName>
            <shortName evidence="2">TS</shortName>
            <shortName evidence="2">TSase</shortName>
            <ecNumber evidence="2">2.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">thyA</name>
        <name evidence="4" type="ordered locus">Metig_0173</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">May catalyze the biosynthesis of dTMP using an unknown cosubstrate.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Pyrimidine metabolism; dTTP biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the thymidylate synthase family. Archaeal-type ThyA subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="2"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95730.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798339.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0173"/>
    <dbReference type="EnsemblBacteria" id="AEF95730">
        <property type="protein sequence ID" value="AEF95730"/>
        <property type="gene ID" value="Metig_0173"/>
    </dbReference>
    <dbReference type="GeneID" id="10643008"/>
    <dbReference type="KEGG" id="mig:Metig_0173"/>
    <dbReference type="HOGENOM" id="CLU_084975_0_0_2"/>
    <dbReference type="OMA" id="HHSVSMH"/>
    <dbReference type="OrthoDB" id="57461at2157"/>
    <dbReference type="UniPathway" id="UPA00575"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004799">
        <property type="term" value="F:thymidylate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006231">
        <property type="term" value="P:dTMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006235">
        <property type="term" value="P:dTTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00351">
        <property type="entry name" value="TS_Pyrimidine_HMase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.572.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01686">
        <property type="entry name" value="Thymidy_synth_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045097">
        <property type="entry name" value="Thymidate_synth/dCMP_Mease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023451">
        <property type="entry name" value="Thymidate_synth/dCMP_Mease_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036926">
        <property type="entry name" value="Thymidate_synth/dCMP_Mease_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014620">
        <property type="entry name" value="Thymidylate_synthase_arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11548">
        <property type="entry name" value="PTHR11548"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00303">
        <property type="entry name" value="Thymidylat_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036752">
        <property type="entry name" value="TSase_MJ051"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55831">
        <property type="entry name" value="SSF55831"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03283">
        <property type="entry name" value="thy_syn_methano"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="2" id="KW-0545">Nucleotide biosynthesis</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Thymidylat_synt" evidence="3">
        <location>
            <begin position="83"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="active site" evidence="2">
        <location>
            <position position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEF95730.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="224" mass="26024" checksum="7CE822C93C0980E8" modified="2011-07-27" version="1">MLCLKQRSVKNAYETLVPKILKEGLEMTTEDGQRCKEIMNVMIEITNPKDKSISPKYPLGERAVENYTQQLLYGAKNEFSYDYHSRLFDYPNEDNTKRINQIQYIIDKLKEQPSSRRCVAVTWNPFIDVKVSMQERGSVPCLNHLQIIRRGDHIYMTVLFRSNDILCAFVANAIGLITLGEIIAEETGTELKKYVHIANSAHIYVDRDRDYIKKYFPDCVDCIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEZ5</accession>
    <name>F6BEZ5_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">NYN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0174</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95731.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798340.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0174"/>
    <dbReference type="EnsemblBacteria" id="AEF95731">
        <property type="protein sequence ID" value="AEF95731"/>
        <property type="gene ID" value="Metig_0174"/>
    </dbReference>
    <dbReference type="GeneID" id="10643009"/>
    <dbReference type="KEGG" id="mig:Metig_0174"/>
    <dbReference type="HOGENOM" id="CLU_100862_0_0_2"/>
    <dbReference type="OMA" id="TIAYVTR"/>
    <dbReference type="OrthoDB" id="73876at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004540">
        <property type="term" value="F:ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002790">
        <property type="entry name" value="CHP00288"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021139">
        <property type="entry name" value="NYN_MARF1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01936">
        <property type="entry name" value="NYN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00288">
        <property type="entry name" value="TIGR00288"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NYN" evidence="1">
        <location>
            <begin position="23"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF95731.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="183" mass="20982" checksum="E12EE44FD47A3A46" modified="2011-07-27" version="1">MWKKLGDLKSKLYIKTRKKGSNKIALLIDGPNMLRKEFNIDLDKIREALEEFGTIVIGRVYLNQYASDKLIEAIVNQGFEPRISAGDVDVEMAVDGTELIFNDNIDTIAYMTRDADFLPAIRKAKERGKKIIVIGAEPGFSTALQNIADHVIRIEENFALDREKLEKKKKERELINKEREDEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-09-29" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F6BF01</accession>
    <name>F6BF01_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ATPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0180</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95737.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798346.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF95737">
        <property type="protein sequence ID" value="AEF95737"/>
        <property type="gene ID" value="Metig_0180"/>
    </dbReference>
    <dbReference type="GeneID" id="10643015"/>
    <dbReference type="KEGG" id="mig:Metig_0180"/>
    <dbReference type="HOGENOM" id="CLU_068608_0_0_2"/>
    <dbReference type="OMA" id="NKKGVQP"/>
    <dbReference type="OrthoDB" id="50712at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011579">
        <property type="entry name" value="ATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01637">
        <property type="entry name" value="ATPase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATPase_2" evidence="1">
        <location>
            <begin position="3"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF95737.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="361" mass="43011" checksum="B33FBF825D9F0F2B" modified="2011-07-27" version="1">MKFFNREKEINEILHILSKESDDIYFIYGPLNSGKTALINHIINNELKKFNKKYAVFYVNFREYDIFSMDNFVEALFEIDENSEEKTMKSYIESFTKSVNDIINLYYGIKVPEPILNKLFGEKEKGRLVFKFIRDLFISLNKKGVQPVFILDELQMIKDIVMNGEKPLLKSLFQFLVSLTKERHIAYCLCLSSDSLFIEYVYNIGELEGRAKYLLVDDFDKETTLKFIDFLTKEKLNKTLSDGDRELIYSYVGGKPKDIKYVVEESKFKDLREILEHMLRDEIQKLKYFLEDVKEEDEEFYKKIVDVLRIFKENYEVEDTIINKKIREFLIKRNILFLNPQKGTLKPQSYLVWNAIKRVIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>F6BF07</accession>
    <name>F6BF07_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uroporphyrinogen-III synthase</fullName>
            <ecNumber evidence="2">4.2.1.75</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0186</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EC" id="4.2.1.75" evidence="2"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95743.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798352.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0186"/>
    <dbReference type="EnsemblBacteria" id="AEF95743">
        <property type="protein sequence ID" value="AEF95743"/>
        <property type="gene ID" value="Metig_0186"/>
    </dbReference>
    <dbReference type="GeneID" id="10643021"/>
    <dbReference type="KEGG" id="mig:Metig_0186"/>
    <dbReference type="HOGENOM" id="CLU_011276_9_5_2"/>
    <dbReference type="OMA" id="VWAVGKT"/>
    <dbReference type="OrthoDB" id="106625at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004852">
        <property type="term" value="F:uroporphyrinogen-III synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006780">
        <property type="term" value="P:uroporphyrinogen III biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06578">
        <property type="entry name" value="HemD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10090">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036108">
        <property type="entry name" value="4pyrrol_syn_uPrphyn_synt_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003754">
        <property type="entry name" value="4pyrrol_synth_uPrphyn_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039793">
        <property type="entry name" value="UROS/Hem4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40082">
        <property type="entry name" value="PTHR40082"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02602">
        <property type="entry name" value="HEM4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69618">
        <property type="entry name" value="SSF69618"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HEM4" evidence="1">
        <location>
            <begin position="15"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF95743.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="243" mass="28068" checksum="C82AFF560285FBAE" modified="2011-07-27" version="1">MKVVITRPRERASYFAKLLEDNGFEPILIPTLELVYKKDVKIDVDKYDWILFTSPSGVKGLYDIIDDKEKEKVKNKKIGAIGIKTAKAFEKYFGKYPDVVPKEYTAEGLLEELRKMINGDERFLIPTTPSTRDVLKNNLNADLFFVYTSEEPKDIKEKVEKLKEIIKEESKKNKRIILTFTSGLTARNFFKNADNELKELLKNQYIVAIGPVTKKEINKFGFNALTPKEYTIEGMLEVIKNLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2020-06-17" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>F6BF12</accession>
    <name>F6BF12_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_0191</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95748.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF95748">
        <property type="protein sequence ID" value="AEF95748"/>
        <property type="gene ID" value="Metig_0191"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0191"/>
    <dbReference type="HOGENOM" id="CLU_3194505_0_0_2"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF95748.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="44" mass="4827" checksum="C1B6659E0DFEB9F5" modified="2011-07-27" version="1">MPLMKVDTGDLRRRTLLSVKPMGGAGVLPERSDGNLKTVRFLCP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F6BF17</accession>
    <name>F6BF17_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_0196</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95753.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0196"/>
    <dbReference type="EnsemblBacteria" id="AEF95753">
        <property type="protein sequence ID" value="AEF95753"/>
        <property type="gene ID" value="Metig_0196"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0196"/>
    <dbReference type="HOGENOM" id="CLU_067259_0_0_2"/>
    <dbReference type="OMA" id="LTVGTDM"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035402">
        <property type="entry name" value="DUF1611-N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035086">
        <property type="entry name" value="DUF1611_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011669">
        <property type="entry name" value="UCP026760"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40690">
        <property type="entry name" value="PTHR40690"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07755">
        <property type="entry name" value="DUF1611"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17396">
        <property type="entry name" value="DUF1611_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF1611_N" evidence="2">
        <location>
            <begin position="24"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="domain" description="DUF1611" evidence="1">
        <location>
            <begin position="109"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF17396"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF95753.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="308" mass="34362" checksum="14AF21C590211E31" modified="2011-07-27" version="1">MIERAKISHENKVSDGLKKFSNIIIDERNYDTFIWTKEILYKEDLKIWAESIASEIEKGKNIYNMARLYRVNDVKSLMDLAYSYGVEVFDTSNPQRFKELRKFAEEGLKGIKSKVITIMGTGRQCGKFTTSFILKKELEKRGYSVGVVGTDPHALLCGADEMVIPQVIEACHVAPTIFGAVKKVDLMDKDVIIVSSQTGILADALEVGTGRGGGVISTAILMGSKPDFVILATKETNIELIKKNIKIIELLSDKEVIGITFNSKNLGHEETKRTIEHLSSKLNLPIADVVKNINLKELIDCVEEHISK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>F6BF54</accession>
    <name>F6BF54_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Tetratricopeptide TPR_1 repeat-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metig_1389</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96924.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799520.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1389"/>
    <dbReference type="EnsemblBacteria" id="AEF96924">
        <property type="protein sequence ID" value="AEF96924"/>
        <property type="gene ID" value="Metig_1389"/>
    </dbReference>
    <dbReference type="GeneID" id="10644262"/>
    <dbReference type="KEGG" id="mig:Metig_1389"/>
    <dbReference type="HOGENOM" id="CLU_014263_0_0_2"/>
    <dbReference type="OrthoDB" id="104509at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001440">
        <property type="entry name" value="TPR_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_repeat"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00515">
        <property type="entry name" value="TPR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="SSF48452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="2">
        <location>
            <begin position="96"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="2">
        <location>
            <begin position="220"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="469"/>
            <end position="496"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEF96924.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="705" mass="84398" checksum="9B303B7B88A81DDE" modified="2011-07-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>F6BF58</accession>
    <name>F6BF58_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Beta-lactamase domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_1393</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96928.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799524.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1393"/>
    <dbReference type="EnsemblBacteria" id="AEF96928">
        <property type="protein sequence ID" value="AEF96928"/>
        <property type="gene ID" value="Metig_1393"/>
    </dbReference>
    <dbReference type="GeneID" id="10644266"/>
    <dbReference type="KEGG" id="mig:Metig_1393"/>
    <dbReference type="HOGENOM" id="CLU_031965_0_0_2"/>
    <dbReference type="OMA" id="HGYHPDW"/>
    <dbReference type="OrthoDB" id="12902at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011108">
        <property type="entry name" value="RMMBL"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12706">
        <property type="entry name" value="Lactamase_B_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07521">
        <property type="entry name" value="RMMBL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="SSF56281"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Lactamase_B" evidence="2">
        <location>
            <begin position="86"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="domain" description="RMMBL" evidence="1">
        <location>
            <begin position="458"/>
            <end position="488"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07521"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF96928.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="511" mass="59019" checksum="834F79A50AA78BED" modified="2011-07-27" version="1">MKITIYDGANTIGGNKIYIKEGDNGLFLDFGMNFANYSKYYEEYLNERSARGIYDLWHLNLIPKLNIYRSDLIPKDLDIYRYPKIPINAVLISHAHLDHVGNIAILNEEIPMVGSSITMTILKALRDTSTGKHLGMDLPYYSIKKPKDESGHVLESDRKSPYCSRKIILTDDNKNIEEFIFYRPGQNSSNKVKKINQNEVKTLYEEDLGLEIKAFEVDHSIYGAVGYIVEGDVSVAYTGDFREHGKNKNKTRKFIKEAKNASVLITEGTRVNRDNDVNVSEEDVYNNSLKIIEDAKGLVIADFSPRNFERLEIFKEIAKKTGRELVITTKDAYFLWALKEVANIDIIDDDVKIYENLKATQQKWEKEILDLYCNYIISPFEIRENCENYILCFSFYDMPHLLDVNPDGGIYIYSSSEAFGEEQEFSFLRLWNWLKHFNFEIYGFKVDENGKPIFEKGLHTSGHISKEGLREVIEKIDPDYIIPVHTENPELFRWAFGESVVLLNNGESLEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>F6BF67</accession>
    <name>F6BF67_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="3">AIG2 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_1402</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the gamma-glutamylcyclotransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96937.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799533.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1402"/>
    <dbReference type="EnsemblBacteria" id="AEF96937">
        <property type="protein sequence ID" value="AEF96937"/>
        <property type="gene ID" value="Metig_1402"/>
    </dbReference>
    <dbReference type="GeneID" id="10644275"/>
    <dbReference type="KEGG" id="mig:Metig_1402"/>
    <dbReference type="HOGENOM" id="CLU_083466_5_1_2"/>
    <dbReference type="OMA" id="KEKYAMY"/>
    <dbReference type="OrthoDB" id="114717at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061929">
        <property type="term" value="F:gamma-glutamylaminecyclotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06661">
        <property type="entry name" value="GGCT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009288">
        <property type="entry name" value="AIG2-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039126">
        <property type="entry name" value="GGACT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013024">
        <property type="entry name" value="GGCT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036568">
        <property type="entry name" value="GGCT-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12510">
        <property type="entry name" value="PTHR12510"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06094">
        <property type="entry name" value="GGACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110857">
        <property type="entry name" value="SSF110857"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GGACT" evidence="2">
        <location>
            <begin position="4"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF06094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF96937.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="120" mass="14243" checksum="3FEE5415F696C92C" modified="2011-07-27" version="1">MEYVFVYGTLRRGLWNNRLLKNSKFIGKGKTKEKYAMYADIIPYVVEDEKVSHIVGEVYEVDEETLERIDALEGHPYHYKRKKVPIILENGEEIEAWLYFYPEPYGTLVKSGDYKDYYEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>F6BA82</accession>
    <name>F6BA82_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">CRISPR-associated endoribonuclease Cas2</fullName>
            <ecNumber evidence="8">3.1.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">cas2</name>
        <name evidence="9" type="ordered locus">Metig_0215</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Functions as a ssRNA-specific endoribonuclease. Involved in the integration of spacer DNA into the CRISPR cassette.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 8">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer, forms a heterotetramer with a Cas1 homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the CRISPR-associated endoribonuclease Cas2 protein family.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="8"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95772.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798381.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0215"/>
    <dbReference type="EnsemblBacteria" id="AEF95772">
        <property type="protein sequence ID" value="AEF95772"/>
        <property type="gene ID" value="Metig_0215"/>
    </dbReference>
    <dbReference type="GeneID" id="10643050"/>
    <dbReference type="KEGG" id="mig:Metig_0215"/>
    <dbReference type="HOGENOM" id="CLU_161124_0_1_2"/>
    <dbReference type="OMA" id="LTHVQNS"/>
    <dbReference type="OrthoDB" id="118787at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:endoribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043571">
        <property type="term" value="P:maintenance of CRISPR repeat elements"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd09725">
        <property type="entry name" value="Cas2_I_II_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01471">
        <property type="entry name" value="Cas2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021127">
        <property type="entry name" value="CRISPR_associated_Cas2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019199">
        <property type="entry name" value="Virulence_VapD/CRISPR_Cas2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34405">
        <property type="entry name" value="PTHR34405"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09827">
        <property type="entry name" value="CRISPR_Cas2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01573">
        <property type="entry name" value="cas2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0051">Antiviral defense</keyword>
    <keyword evidence="4 8" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="5 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 8" id="KW-0540">Nuclease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="8">
        <location>
            <position position="8"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AEF95772.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="85" mass="10026" checksum="FDF7E0923FE482B0" modified="2011-07-27" version="1">MYVVIVYDVNVSRVNKVKKFLRQHLNWVQNSVFEGEITKAEFERIKDGLLNIIDENEDSIIIYKLKSKPSREIYGIEKNPIDDII</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2020-06-17" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAB1</accession>
    <name>F6BAB1_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_0244</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95801.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF95801">
        <property type="protein sequence ID" value="AEF95801"/>
        <property type="gene ID" value="Metig_0244"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0244"/>
    <dbReference type="HOGENOM" id="CLU_3245331_0_0_2"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF95801.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="42" mass="5418" checksum="9DF32C0A7AF7B735" modified="2011-07-27" version="1">MKDFLYYLIRYSKIFSRENLYKFLDDSIKKYTLMFIQDYLYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAD8</accession>
    <name>F6BAD8_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Amino acid-binding ACT domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0272</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95828.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798437.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0272"/>
    <dbReference type="EnsemblBacteria" id="AEF95828">
        <property type="protein sequence ID" value="AEF95828"/>
        <property type="gene ID" value="Metig_0272"/>
    </dbReference>
    <dbReference type="GeneID" id="10643107"/>
    <dbReference type="KEGG" id="mig:Metig_0272"/>
    <dbReference type="HOGENOM" id="CLU_1264587_0_0_2"/>
    <dbReference type="OMA" id="YTQQFIK"/>
    <dbReference type="OrthoDB" id="89825at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002912">
        <property type="entry name" value="ACT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040537">
        <property type="entry name" value="DUF5612"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015832">
        <property type="entry name" value="UCP006363_ACT"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18462">
        <property type="entry name" value="DUF5612"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006363">
        <property type="entry name" value="UCP006363_ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="SSF55021"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51671">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ACT" evidence="1">
        <location>
            <begin position="4"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF95828.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="217" mass="23475" checksum="D9A47A70FC91EFA6" modified="2011-07-27" version="1">MEIGLTIMAENKIGVLHKLTGIILEFNGNITYTQQFIKDDNIGLIYMEIEGISDEEGLLKKIRECSFVKSVEVHKTLKRIYGKRVIIIGGGAQVAQVAQGAISEADRHNIRGERISVDTMPIVGEENLRDAVLAVEKLPRAAILVLAGSLMGGEITKAVEELKKKTGIPVISLKMFGSVPKVADLVVSDPVQAGVLAVMAIADTAKFDIEKVKGRVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAE3</accession>
    <name>F6BAE3_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Precorrin-4 C11-methyltransferase</fullName>
            <ecNumber evidence="9">2.1.1.133</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Metig_1421</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; adenosylcobalamin biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the precorrin methyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.133" evidence="9"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96956.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799552.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1421"/>
    <dbReference type="EnsemblBacteria" id="AEF96956">
        <property type="protein sequence ID" value="AEF96956"/>
        <property type="gene ID" value="Metig_1421"/>
    </dbReference>
    <dbReference type="GeneID" id="10644294"/>
    <dbReference type="KEGG" id="mig:Metig_1421"/>
    <dbReference type="HOGENOM" id="CLU_011276_7_1_2"/>
    <dbReference type="OMA" id="WSAMGEQ"/>
    <dbReference type="OrthoDB" id="67633at2157"/>
    <dbReference type="UniPathway" id="UPA00148"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046026">
        <property type="term" value="F:precorrin-4 C11-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019354">
        <property type="term" value="P:siroheme biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11641">
        <property type="entry name" value="Precorrin-4_C11-MT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.950.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1010.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000878">
        <property type="entry name" value="4pyrrol_Mease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035996">
        <property type="entry name" value="4pyrrol_Methylase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014777">
        <property type="entry name" value="4pyrrole_Mease_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014776">
        <property type="entry name" value="4pyrrole_Mease_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006362">
        <property type="entry name" value="Cbl_synth_CobM/CibF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006366">
        <property type="entry name" value="CobA/CysG_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003043">
        <property type="entry name" value="Uropor_MeTrfase_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00590">
        <property type="entry name" value="TP_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53790">
        <property type="entry name" value="SSF53790"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01469">
        <property type="entry name" value="cobA_cysG_Cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01465">
        <property type="entry name" value="cobM_cbiF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00839">
        <property type="entry name" value="SUMT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00840">
        <property type="entry name" value="SUMT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6 9" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4 6 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="TP_methylase" evidence="7">
        <location>
            <begin position="6"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="domain" description="Radical SAM core" evidence="8">
        <location>
            <begin position="54"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AEF96956.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="256" mass="28570" checksum="66B9175821298C2B" modified="2011-07-27" version="1">MDDCKKVVIVGAGPGDPELITLKGKKAIENADVIIYAGSLVNKELLKYNKKNARIYNSATMDLEEIVKIMVENVKKGLNVVRLHTGDPSIYGAIKEQIDELKKHGIDVEIIPGVTSLFAAAASLKVELTLPDVSQTVIITRPEGRTPMPEKEKLRDLAKHNATMAIYLGVSMIDKVCRELIEGGYKEDTPVAVVYHASWEDEKIIVGTLKDIAEKVKKEGIKKTALIIVGEVLNPRHYAYSKLYDKEFEHEYRKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAF0</accession>
    <name>F6BAF0_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_1428</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96963.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799559.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96963">
        <property type="protein sequence ID" value="AEF96963"/>
        <property type="gene ID" value="Metig_1428"/>
    </dbReference>
    <dbReference type="GeneID" id="10644301"/>
    <dbReference type="KEGG" id="mig:Metig_1428"/>
    <dbReference type="HOGENOM" id="CLU_1076106_0_0_2"/>
    <dbReference type="OMA" id="FCKPFDI"/>
    <dbReference type="OrthoDB" id="62876at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF96963.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="256" mass="29481" checksum="BD14DCBAFEBAB9AD" modified="2011-07-27" version="1">MIEVIVYEELRGNKKDVVEEEFEKAINELKEKYNAKLLSIENEEEGNLYTKVGELEIKFESFLDYIDFCLKHGADVEVISPPKLKMDSKEFGNAIAHIIQFFSNFCKKYNVGFNVVVREEKDIDIDKYREGIYDEDDIHELQEEGYLRVKAVFEVEGKSEEVVIKNIILSLDNNIHINKIITKVLEDRAEGFHGLVAVDMLCKPFEIVEIAYKFLPVAVVIEGDKEIELDISELQDIGNELSGAVFELSHAVKLRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAF6</accession>
    <name>F6BAF6_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Histidinol-phosphate aminotransferase</fullName>
            <ecNumber evidence="3">2.6.1.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Imidazole acetol-phosphate transaminase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">hisC</name>
        <name evidence="5" type="ordered locus">Metig_1434</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>2-oxoglutarate + L-histidinol phosphate = 3-(imidazol-4-yl)-2-oxopropyl phosphate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:23744"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57766"/>
            <dbReference type="ChEBI" id="CHEBI:57980"/>
            <dbReference type="EC" id="2.6.1.9"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 3">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 7/9.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family. Histidinol-phosphate aminotransferase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.9" evidence="3"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96969.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799565.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1434"/>
    <dbReference type="EnsemblBacteria" id="AEF96969">
        <property type="protein sequence ID" value="AEF96969"/>
        <property type="gene ID" value="Metig_1434"/>
    </dbReference>
    <dbReference type="GeneID" id="10644307"/>
    <dbReference type="KEGG" id="mig:Metig_1434"/>
    <dbReference type="HOGENOM" id="CLU_017584_3_3_2"/>
    <dbReference type="OMA" id="IWLNANE"/>
    <dbReference type="OrthoDB" id="69863at2157"/>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00012"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004400">
        <property type="term" value="F:histidinol-phosphate transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01023">
        <property type="entry name" value="HisC_aminotrans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005861">
        <property type="entry name" value="HisP_aminotrans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01141">
        <property type="entry name" value="hisC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="2 3 5" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="3" id="KW-0368">Histidine biosynthesis</keyword>
    <keyword evidence="3" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotran_1_2" evidence="4">
        <location>
            <begin position="35"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="3">
        <location>
            <position position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEF96969.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="369" mass="42439" checksum="9AE22CE9517EEC84" modified="2011-07-27" version="1">MIEDKIRDIVKQFKPYVPGKSKEEIARKYGIKPEEIIKLGSNENPWGPSPKIKEEILKEIPKLHQYPEPVSPELLEELSKFLDVPKENIIVGGDGADEVIDTIMRTFIDKGDNVVIPIPTFTQYAISAKIYGAEIRYAKFDKEKDFQLDVESVLNNIDERTKVIFLCTPNNPTGNVIDKKDIEKIINSTDALVVIDHAYIEYAKKEYDLTKWALKYDNVLVLRTFSKVFGLAGQRIGYGITNEKIVNYMMRVKPIFSLTRLSQICAITALRDKEFFEKCVKDGIKSREMLYEGLKKFKDLKVYPSEANYVLVELKTMTSKEFCEELLKRGVIVRDCSSFDGLGDNYVRISIGTFEENERFLKILEEIVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-09-29" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAG7</accession>
    <name>F6BAG7_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="3">DNA polymerase beta domain protein region</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_1445</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96980.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799576.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96980">
        <property type="protein sequence ID" value="AEF96980"/>
        <property type="gene ID" value="Metig_1445"/>
    </dbReference>
    <dbReference type="GeneID" id="10644318"/>
    <dbReference type="KEGG" id="mig:Metig_1445"/>
    <dbReference type="HOGENOM" id="CLU_154345_0_0_2"/>
    <dbReference type="OMA" id="SYAKNEY"/>
    <dbReference type="OrthoDB" id="115826at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041633">
        <property type="entry name" value="Polbeta"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18765">
        <property type="entry name" value="Polbeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="SSF81301"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Polbeta" evidence="2">
        <location>
            <begin position="3"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF18765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF96980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="136" mass="16514" checksum="B99EA728D9E2A2BE" modified="2011-07-27" version="1">MIEKIKKDFEEFKDKAFGILLYGSYAKNEYTERSDVDICLVGAEKDIYLEILGKLGNKYDIKIFEDLPLYIKMDVIKNHKVIWGNELELSEYFYKFRKTWKDMEKRIRENQFESVREKVFLRRRFNGKKKEIFGKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAI9</accession>
    <name>F6BAI9_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Phosphoesterase RecJ domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_1468</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97002.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799598.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1468"/>
    <dbReference type="EnsemblBacteria" id="AEF97002">
        <property type="protein sequence ID" value="AEF97002"/>
        <property type="gene ID" value="Metig_1468"/>
    </dbReference>
    <dbReference type="GeneID" id="10644341"/>
    <dbReference type="KEGG" id="mig:Metig_1468"/>
    <dbReference type="HOGENOM" id="CLU_042622_0_0_2"/>
    <dbReference type="OMA" id="ACGAYIP"/>
    <dbReference type="OrthoDB" id="45290at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001667">
        <property type="entry name" value="DDH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038763">
        <property type="entry name" value="DHH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003156">
        <property type="entry name" value="DHHA1_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01368">
        <property type="entry name" value="DHH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02272">
        <property type="entry name" value="DHHA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64182">
        <property type="entry name" value="SSF64182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DHH" evidence="1">
        <location>
            <begin position="18"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="domain" description="DHHA1" evidence="2">
        <location>
            <begin position="335"/>
            <end position="459"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF97002.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="460" mass="52986" checksum="6226B4BC3E4DFB71" modified="2011-07-27" version="1">MENLKRAVKTLRKNKDKNILICTHIDTDGITSRIILEKLMERLNIDADFMFLRQINVNSIEEIPFSDYDLIIFADLGSGQLSLIKEKIENTNKKVIILDHHMVENVKIPENIINVNPWNLNKDGGKEICGAGVCYFFAKICNPRWTDLAKYAVLGSIGDIQNFEGKLKGLNEKILKDAIERGDVKKYMDLQFYGKQTRPLFISMKYFTDVRTDLVNNDSRIIRFIMNVGEKYNLDINPTLSLCEIPFECKRALGSEFLHKCNKYVPREWSIYLPKVIFGEVYEFVHEEFKTPLRDLEEFSTCINACARYGEYEIALNVLRGDRESYYKKMLSMLRKHRKNLAKSLNHVKDEVEVIQKDKFQYFETDKIKAEIVGIVASLTYSLEKVDWKKPIFAIADNGDGYKVSARCPKLLAFAKDINLADAIRYASYKVGGSGGGHKFACGAYVPDVDEFIKHLEKRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAL2</accession>
    <name>F6BAL2_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">2-phospho-L-lactate transferase</fullName>
            <ecNumber evidence="3">2.7.8.28</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">EPPG:FO PEP transferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">cofD</name>
        <name evidence="4" type="ordered locus">Metig_1491</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the transfer of the 2-phospholactate moiety from (2S)-lactyl-2-diphospho-5'-guanosine to 7,8-didemethyl-8-hydroxy-5-deazariboflavin (FO) with the formation of oxidized coenzyme F420-0 and GMP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>(2S)-lactyl-2-diphospho-5'-guanosine + 7,8-didemethyl-8-hydroxy-5-deazariboflavin = GMP + H(+) + oxidized coenzyme F420-0</text>
            <dbReference type="Rhea" id="RHEA:63444"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
            <dbReference type="ChEBI" id="CHEBI:59435"/>
            <dbReference type="ChEBI" id="CHEBI:59904"/>
            <dbReference type="ChEBI" id="CHEBI:59907"/>
            <dbReference type="EC" id="2.7.8.28"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Cofactor biosynthesis; coenzyme F420 biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CofD family.</text>
    </comment>
    <dbReference type="EC" id="2.7.8.28" evidence="3"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97025.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799620.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1491"/>
    <dbReference type="EnsemblBacteria" id="AEF97025">
        <property type="protein sequence ID" value="AEF97025"/>
        <property type="gene ID" value="Metig_1491"/>
    </dbReference>
    <dbReference type="GeneID" id="10644364"/>
    <dbReference type="KEGG" id="mig:Metig_1491"/>
    <dbReference type="HOGENOM" id="CLU_055795_1_0_2"/>
    <dbReference type="OMA" id="DLDTVMY"/>
    <dbReference type="OrthoDB" id="31831at2157"/>
    <dbReference type="UniPathway" id="UPA00071"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043743">
        <property type="term" value="F:LPPG:FO 2-phospho-L-lactate transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052645">
        <property type="term" value="P:F420-0 metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07186">
        <property type="entry name" value="CofD_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10680">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01257">
        <property type="entry name" value="CofD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002882">
        <property type="entry name" value="CofD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038136">
        <property type="entry name" value="CofD-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010115">
        <property type="entry name" value="FbiA/CofD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43007">
        <property type="entry name" value="PTHR43007"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01933">
        <property type="entry name" value="CofD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF142338">
        <property type="entry name" value="SSF142338"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01819">
        <property type="entry name" value="F420_cofD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3 4" id="KW-0808">Transferase</keyword>
    <feature type="binding site" evidence="3">
        <location>
            <position position="50"/>
        </location>
        <ligand>
            <name>7,8-didemethyl-8-hydroxy-5-deazariboflavin</name>
            <dbReference type="ChEBI" id="CHEBI:59904"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="89"/>
        </location>
        <ligand>
            <name>7,8-didemethyl-8-hydroxy-5-deazariboflavin</name>
            <dbReference type="ChEBI" id="CHEBI:59904"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEF97025.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="313" mass="34754" checksum="0A571948AC9AA7AA" modified="2011-07-27" version="1">MKISVLSGGTGTPKLIQGLKEVIPQKDISVIVNTGEDTWIGDLYLSPDVDTVLYTLTDLINEETWYGIRGDTFYCHEQLKKLGFDEVLRIGDKDRALKMHKTHFLKKGFKLSEVIDMERKALGIEAEVYPMTDDRVETKILAKEGNEKVLLKFHDFWVKRRGNVDVVDVIYENAQYAKGCEGALKAIEKSDFVLIGPSNPITSIGPILSIKDIKDALNDKFVVAVSPIVGDKAVSGPAGTLMKAKGLSVDVLGVYEFYKDIVDVMVIDEVDADKADKIKCETLVTNTIMKTLNDKINLAKAILEFIRGYGCLC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAM8</accession>
    <name>F6BAM8_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Coenzyme F420 hydrogenase., Selenate reductase</fullName>
            <ecNumber evidence="4">1.12.98.1</ecNumber>
            <ecNumber evidence="4">1.97.1.9</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metig_0286</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.12.98.1" evidence="4"/>
    <dbReference type="EC" id="1.97.1.9" evidence="4"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95842.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798451.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0286"/>
    <dbReference type="EnsemblBacteria" id="AEF95842">
        <property type="protein sequence ID" value="AEF95842"/>
        <property type="gene ID" value="Metig_0286"/>
    </dbReference>
    <dbReference type="GeneID" id="25395053"/>
    <dbReference type="KEGG" id="mig:Metig_0286"/>
    <dbReference type="HOGENOM" id="CLU_428041_0_0_2"/>
    <dbReference type="OrthoDB" id="105930at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050454">
        <property type="term" value="F:coenzyme F420 hydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033797">
        <property type="term" value="F:selenate reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007516">
        <property type="entry name" value="Co_F420_Hydgase/DH_bsu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045220">
        <property type="entry name" value="FRHB/FDHB/HCAR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007525">
        <property type="entry name" value="FrhB_FdhB_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31332">
        <property type="entry name" value="PTHR31332"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00037">
        <property type="entry name" value="Fer4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13247">
        <property type="entry name" value="Fer4_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04432">
        <property type="entry name" value="FrhB_FdhB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04422">
        <property type="entry name" value="FrhB_FdhB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0408">Iron</keyword>
    <keyword evidence="2" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="3">
        <location>
            <begin position="5"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="3">
        <location>
            <begin position="375"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="3">
        <location>
            <begin position="409"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="3">
        <location>
            <begin position="440"/>
            <end position="469"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEF95842.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="534" mass="60683" checksum="15C45692D8BB9D63" modified="2011-07-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAP6</accession>
    <name>F6BAP6_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0304</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95860.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0304"/>
    <dbReference type="EnsemblBacteria" id="AEF95860">
        <property type="protein sequence ID" value="AEF95860"/>
        <property type="gene ID" value="Metig_0304"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0304"/>
    <dbReference type="HOGENOM" id="CLU_037290_0_0_2"/>
    <dbReference type="OMA" id="LYKMEME"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001646">
        <property type="entry name" value="5peptide_repeat"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13576">
        <property type="entry name" value="Pentapeptide_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="564"/>
            <end position="585"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF95860.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="635" mass="74759" checksum="9AFC846BB2E65AD6" modified="2011-07-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAQ0</accession>
    <name>F6BAQ0_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">DsrE family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0308</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95864.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798473.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0308"/>
    <dbReference type="EnsemblBacteria" id="AEF95864">
        <property type="protein sequence ID" value="AEF95864"/>
        <property type="gene ID" value="Metig_0308"/>
    </dbReference>
    <dbReference type="GeneID" id="10643143"/>
    <dbReference type="KEGG" id="mig:Metig_0308"/>
    <dbReference type="HOGENOM" id="CLU_088479_0_0_2"/>
    <dbReference type="OMA" id="KPTIFLM"/>
    <dbReference type="OrthoDB" id="72889at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.110.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027396">
        <property type="entry name" value="DsrEFH-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003787">
        <property type="entry name" value="Sulphur_relay_DsrE/F-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001455">
        <property type="entry name" value="TusA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036868">
        <property type="entry name" value="TusA-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02635">
        <property type="entry name" value="DrsE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01206">
        <property type="entry name" value="TusA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64307">
        <property type="entry name" value="SSF64307"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75169">
        <property type="entry name" value="SSF75169"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01148">
        <property type="entry name" value="UPF0033"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UPF0033" evidence="1">
        <location>
            <begin position="73"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS01148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF95864.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="272" mass="29780" checksum="199DD516A1DC6F91" modified="2011-07-27" version="1">MIREISVKGLRSPSLLVDRVLEKMKDGILVIETDGEEQIKDIENLMKKIGYKMETDGNVVKIMVGEIKADKTINVVGATCPGPIMMVSDMLDKMNVGEVLEIIAGKNALTDLTEGLKGAGHEVLSVEELGDGNYRILIKKGEKKAEAVASVTIDELFIINTTGTGNAEKAYATFMMADIAKKMNLKPTIFLMMDGASLALKGECDKVKHPDFPKLGDLVRMAIKNGIKVYVCELSAKFRGINEKNLEDGFEIAGAPTFLNYLSKPNVRPVWL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-06-02" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAQ1</accession>
    <name>F6BAQ1_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_0309</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95865.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF95865">
        <property type="protein sequence ID" value="AEF95865"/>
        <property type="gene ID" value="Metig_0309"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0309"/>
    <dbReference type="HOGENOM" id="CLU_197247_0_0_2"/>
    <dbReference type="OMA" id="ELCIKCE"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF95865.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="70" mass="8276" checksum="E0B1FE57E22BC83E" modified="2011-07-27" version="1">MYEYGTYVIKMELKIICKHSKFSENGDNIHVICEFCGGRDLKTEIELLKEDFEIKKISYPNCEILKRVVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAQ9</accession>
    <name>F6BAQ9_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Nucleoside recognition domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_0317</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95873.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798482.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0317"/>
    <dbReference type="EnsemblBacteria" id="AEF95873">
        <property type="protein sequence ID" value="AEF95873"/>
        <property type="gene ID" value="Metig_0317"/>
    </dbReference>
    <dbReference type="GeneID" id="10643153"/>
    <dbReference type="KEGG" id="mig:Metig_0317"/>
    <dbReference type="HOGENOM" id="CLU_048086_2_0_2"/>
    <dbReference type="OMA" id="YGIWGAE"/>
    <dbReference type="OrthoDB" id="68115at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011642">
        <property type="entry name" value="Gate_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038880">
        <property type="entry name" value="MJ0871-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38139">
        <property type="entry name" value="PTHR38139"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07670">
        <property type="entry name" value="Gate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="60"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="113"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="187"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="227"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="259"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="296"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="domain" description="Gate" evidence="2">
        <location>
            <begin position="23"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF95873.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="319" mass="35550" checksum="2EF722F54CEF7EEB" modified="2011-07-27" version="1">MDLEFYLNALINTLKISTLYTVKISIIVLITMFIVNYIMNTGIMKKVSDLLSPILKRLKINPLSISSILACFFSPTVGYSILAEGLKENKINEKEIIGTSLANSFPSVLSHTFTFFIPVVIPILGITGVLFVLIRLGVAFTKTIIGFLYLSIISKDCNFEMPNIGKMDKKENVKKSFKSTLRFAKRLIPVMFFMMTLVVYLSKIGFFDYVKRFVEPITNFLHLNPNVGILALTEIMNVHAAIVMAGGFLNEGILSSKEVLIGLIIGNVITLSSRYAKHSLPLHVSLFGAKLGTKIVIINAAITLLLDILIIIWLLLFHN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAY4</accession>
    <name>F6BAY4_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Tryptophan synthase beta chain</fullName>
            <ecNumber evidence="12">4.2.1.20</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">trpB</name>
        <name evidence="14" type="ordered locus">Metig_1537</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3 12">The beta subunit is responsible for the synthesis of L-tryptophan from indole and L-serine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 12">
            <text>(1S,2R)-1-C-(indol-3-yl)glycerol 3-phosphate + L-serine = D-glyceraldehyde 3-phosphate + H2O + L-tryptophan</text>
            <dbReference type="Rhea" id="RHEA:10532"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:57912"/>
            <dbReference type="ChEBI" id="CHEBI:58866"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="4.2.1.20"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 12">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4 12">Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 5/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6 12">Tetramer of two alpha and two beta chains.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5 12">Belongs to the TrpB family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.20" evidence="12"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97071.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799665.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1537"/>
    <dbReference type="EnsemblBacteria" id="AEF97071">
        <property type="protein sequence ID" value="AEF97071"/>
        <property type="gene ID" value="Metig_1537"/>
    </dbReference>
    <dbReference type="GeneID" id="10644410"/>
    <dbReference type="KEGG" id="mig:Metig_1537"/>
    <dbReference type="HOGENOM" id="CLU_016734_3_1_2"/>
    <dbReference type="OMA" id="HGMKSYF"/>
    <dbReference type="OrthoDB" id="24741at2157"/>
    <dbReference type="UniPathway" id="UPA00035">
        <property type="reaction ID" value="UER00044"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004834">
        <property type="term" value="F:tryptophan synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06446">
        <property type="entry name" value="Trp-synth_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00133">
        <property type="entry name" value="Trp_synth_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="PLP-dep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006653">
        <property type="entry name" value="Trp_synth_b_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006654">
        <property type="entry name" value="Trp_synth_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023026">
        <property type="entry name" value="Trp_synth_beta/beta-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="Trypto_synt_PLP_dependent"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00291">
        <property type="entry name" value="PALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001413">
        <property type="entry name" value="Trp_syn_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="SSF53686"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00263">
        <property type="entry name" value="trpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00168">
        <property type="entry name" value="TRP_SYNTHASE_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 12" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="10 12" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="11 12 14" id="KW-0456">Lyase</keyword>
    <keyword evidence="9 12" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 12" id="KW-0822">Tryptophan biosynthesis</keyword>
    <feature type="domain" description="PALP" evidence="13">
        <location>
            <begin position="60"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="12">
        <location>
            <position position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00002786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00009982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00011270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="AEF97071.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="401" mass="44500" checksum="B3E15CFE5854E773" modified="2011-07-27" version="1">MKKYKDKYPDKNGKYGIYGGKFVPETLMPAIEELEEAFTRFWINNEGNFREEFEYYLREYVGRPTPLYYAKRLSELLGCKVYLKREDLAHLGAHKINNAIGQALLAKRMGKTRIIAETGAGQHGIATAAAATNLGLECVIYMGKKDMERQKLNVFRMELMGAKVVPVVSGSQTLKDAVNEALRDWVTNVRNTHYLIGSTLGPHPYPMMVREFQRVIGKEIKQQILEKEGRLPTCILACVGGGSNSIGAFYEFLDDDVELYAVEAGGKGIETGKHGASLCAGRVGVLHGTKMYVKEDEFGQIEESYSISAGLDYPGVSPELSFLKDEERIKAVYVTDDEALEAFQLLCKLEGILPALESSHAVAYAFKLAEELDKDDIMVINLSGRGDKDVQTVAKALGKIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>F6BB03</accession>
    <name>F6BB03_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Peptidase U32</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_1556</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97090.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799684.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1556"/>
    <dbReference type="EnsemblBacteria" id="AEF97090">
        <property type="protein sequence ID" value="AEF97090"/>
        <property type="gene ID" value="Metig_1556"/>
    </dbReference>
    <dbReference type="GeneID" id="10644429"/>
    <dbReference type="KEGG" id="mig:Metig_1556"/>
    <dbReference type="HOGENOM" id="CLU_011540_0_1_2"/>
    <dbReference type="OMA" id="YGGVSHF"/>
    <dbReference type="OrthoDB" id="8700at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071704">
        <property type="term" value="P:organic substance metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001539">
        <property type="entry name" value="Peptidase_U32"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01136">
        <property type="entry name" value="Peptidase_U32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="SSF50447"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01276">
        <property type="entry name" value="PEPTIDASE_U32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF97090.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="406" mass="46279" checksum="9881AA0AE3475169" modified="2011-07-27" version="1">MVELLSPARDLACLTAAIKNGADAVYCGLKDLNMRITAKNFERDELKEGVKFAHDNNKKLYLCTNTVIYENDLKKLEEIFDFANSVEVDAVIVSDLGAMQLAREYGLKVHASVQCNITNSLAAKFYSKFAKRVILSRELTLNQIKEIRENLRKDGINLELEGFVHGALCVAVSGRCFLSSYLFNRHANCGDCLQPCRRKWKLINEHVDGTHEVVCEGKYLLSPKDLCMIEYIPQLMEVLDCFKIEGRAKNADYVMRVTKIYREAIDSVLDGTYKEKLPYFKKELEKVYNRGYDTGFYFRDINKSHDFQYEKEGNISKYKKIEVGRVVNYYKKIGVAEIKLINDLRVGDTILIIGKTTGCVEEVVKSMQINHKNVEIGRKGENVGVKLNHVVREGDKVFVLLPSHNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>F6BB04</accession>
    <name>F6BB04_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Phosphonate-transporting ATPase</fullName>
            <ecNumber evidence="5">3.6.3.28</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Metig_1557</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.6.3.28" evidence="5"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97091.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799685.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1557"/>
    <dbReference type="EnsemblBacteria" id="AEF97091">
        <property type="protein sequence ID" value="AEF97091"/>
        <property type="gene ID" value="Metig_1557"/>
    </dbReference>
    <dbReference type="GeneID" id="10644430"/>
    <dbReference type="KEGG" id="mig:Metig_1557"/>
    <dbReference type="HOGENOM" id="CLU_000604_1_22_2"/>
    <dbReference type="OMA" id="ATMLKPN"/>
    <dbReference type="OrthoDB" id="67630at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03255">
        <property type="entry name" value="ABC_MJ0796_LolCDE_FtsE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017911">
        <property type="entry name" value="MacB_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="4"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="40"/>
            <end position="47"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEF97091.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="225" mass="25565" checksum="B289C513072F026E" modified="2011-07-27" version="1">MSFIEAENIWKSYGKGEAKTVVLKGINLKIEEGEFVAIIGPSGCGKSTLLNILGLLDTPDKGNLYINNKKTTRMSENERARFRRKISGFIFQQFYLINSLTALENVELPMMLEGYSKNYRKRRAKQLLESVDLGDRLYYYPYQLSGGQQQRVAIARALANNPKIIFADEPTGNLDSKRSQQIMDILKNLHDEGITIVMVTHELEYTKYATKIVKMKDGMIVDILG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>F6BB33</accession>
    <name>F6BB33_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Methylated-DNA--[protein]-cysteine S-methyltransferase</fullName>
            <ecNumber evidence="4">2.1.1.63</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Metig_0362</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) and O4-methylthymine (O4-MeT) in DNA. Repairs the methylated nucleobase in DNA by stoichiometrically transferring the methyl group to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 4-O-methyl-thymidine in DNA + L-cysteinyl-[protein] = a thymidine in DNA + S-methyl-L-cysteinyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:53428"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:10132"/>
            <dbReference type="Rhea" id="RHEA-COMP:13555"/>
            <dbReference type="Rhea" id="RHEA-COMP:13556"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:82612"/>
            <dbReference type="ChEBI" id="CHEBI:137386"/>
            <dbReference type="ChEBI" id="CHEBI:137387"/>
            <dbReference type="EC" id="2.1.1.63"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>a 6-O-methyl-2'-deoxyguanosine in DNA + L-cysteinyl-[protein] = a 2'-deoxyguanosine in DNA + S-methyl-L-cysteinyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:24000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:10132"/>
            <dbReference type="Rhea" id="RHEA-COMP:11367"/>
            <dbReference type="Rhea" id="RHEA-COMP:11368"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:82612"/>
            <dbReference type="ChEBI" id="CHEBI:85445"/>
            <dbReference type="ChEBI" id="CHEBI:85448"/>
            <dbReference type="EC" id="2.1.1.63"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.1.1.63" evidence="4"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95918.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0362"/>
    <dbReference type="EnsemblBacteria" id="AEF95918">
        <property type="protein sequence ID" value="AEF95918"/>
        <property type="gene ID" value="Metig_0362"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0362"/>
    <dbReference type="HOGENOM" id="CLU_000445_52_2_2"/>
    <dbReference type="OMA" id="DYFIGMI"/>
    <dbReference type="OrthoDB" id="122341at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003908">
        <property type="term" value="F:methylated-DNA-[protein]-cysteine S-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06445">
        <property type="entry name" value="ATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001497">
        <property type="entry name" value="MethylDNA_cys_MeTrfase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014048">
        <property type="entry name" value="MethylDNA_cys_MeTrfase_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036217">
        <property type="entry name" value="MethylDNA_cys_MeTrfase_DNAb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01035">
        <property type="entry name" value="DNA_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46767">
        <property type="entry name" value="SSF46767"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00589">
        <property type="entry name" value="ogt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00374">
        <property type="entry name" value="MGMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5 9" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DNA_binding_1" evidence="8">
        <location>
            <begin position="81"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00003317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AEF95918.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="161" mass="18517" checksum="967E67D99DDB01A6" modified="2011-07-27" version="1">MEKVVVEINGYFVGFIFKDDVLIRNTIPLSRDEISRFFGWGKVSNKKHHIKMAEYILKLYFGEMLDREVRELINYSLDVSPFTKKVLEVVKDIPYGKVRTYGEIARELNTSPRAVGGAMNKNPLPLIIPCHRVVGKNNIGGYSYGIRHKINILKNEGIIYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-06-02" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>F6BB36</accession>
    <name>F6BB36_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_0365</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95921.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798530.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF95921">
        <property type="protein sequence ID" value="AEF95921"/>
        <property type="gene ID" value="Metig_0365"/>
    </dbReference>
    <dbReference type="GeneID" id="10643202"/>
    <dbReference type="KEGG" id="mig:Metig_0365"/>
    <dbReference type="HOGENOM" id="CLU_157007_0_0_2"/>
    <dbReference type="OMA" id="RNCGFTR"/>
    <dbReference type="OrthoDB" id="105439at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF95921.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="118" mass="14219" checksum="3298E42607247A77" modified="2011-07-27" version="1">MTGVERWVLNYPTKCYHCQRHADQIIEIYTNQAFVKCSNCGATRYYLIRRVAIEEENILELERNKKSKYDIWDLKKEVTCYNCKKDSVQDILVTESKLIVRCRNCGFSRYYWFHMVSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>F6BB39</accession>
    <name>F6BB39_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Mur ligase middle domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0368</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95924.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798533.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0368"/>
    <dbReference type="EnsemblBacteria" id="AEF95924">
        <property type="protein sequence ID" value="AEF95924"/>
        <property type="gene ID" value="Metig_0368"/>
    </dbReference>
    <dbReference type="GeneID" id="10643205"/>
    <dbReference type="KEGG" id="mig:Metig_0368"/>
    <dbReference type="HOGENOM" id="CLU_047362_0_0_2"/>
    <dbReference type="OMA" id="VIAPVHC"/>
    <dbReference type="OrthoDB" id="50390at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009058">
        <property type="term" value="P:biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036565">
        <property type="entry name" value="Mur-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013221">
        <property type="entry name" value="Mur_ligase_cen"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08245">
        <property type="entry name" value="Mur_ligase_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53623">
        <property type="entry name" value="SSF53623"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mur_ligase_M" evidence="1">
        <location>
            <begin position="104"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF95924.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="409" mass="46942" checksum="AF7FDE524E8FABB1" modified="2011-07-27" version="1">MLIVDVNHGALDLAREYIELGYSVDVWDIYGKLEKDKDFAKNYKDIISKVKIIKKDENINFDNYDKIIAPIHCPIEDDFISFHDAVSEIVKEKYGNIHKKFIEVTGVKGKTTTTEMINFILKDDYEVFLHNSNKGSIAPTSILNVLRECNVDDIDYFIFEVSLGITSCGYGVITNIVENYPIAKGKRNALIAKISTLKNADKKYINMKILEEYGLKLNLENLFVIDTNKEIISKYPLKYKFDDYIVEFNKNVFGMHLVENSIFAIKVCENFINTEDIIEKIKNFRINSRMSVEKIKNRYIVKNINPGLDVKAIDYAIKDFLEVFGVKQNPSEPHIKDVVIIGGDFGITCEEIDVKRLAKVIEKYNANFIFVGDIGKELKKNLDKYPFYSKIDLDELDGNVLVIIRSAIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>F6BB47</accession>
    <name>F6BB47_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Pyruvoyl-dependent arginine decarboxylase</fullName>
            <shortName evidence="6">PvlArgDC</shortName>
            <ecNumber evidence="6">4.1.1.19</ecNumber>
        </recommendedName>
        <component>
            <recommendedName>
                <fullName evidence="6">Pyruvoyl-dependent arginine decarboxylase subunit beta</fullName>
            </recommendedName>
        </component>
        <component>
            <recommendedName>
                <fullName evidence="6">Pyruvoyl-dependent arginine decarboxylase subunit alpha</fullName>
            </recommendedName>
        </component>
    </protein>
    <gene>
        <name evidence="6" type="primary">pdaD</name>
        <name evidence="7" type="ordered locus">Metig_0376</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 6">
            <text>H(+) + L-arginine = agmatine + CO2</text>
            <dbReference type="Rhea" id="RHEA:17641"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:32682"/>
            <dbReference type="ChEBI" id="CHEBI:58145"/>
            <dbReference type="EC" id="4.1.1.19"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>pyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
        </cofactor>
        <text evidence="6">Binds 1 pyruvoyl group covalently per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the PdaD family.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.19" evidence="6"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95932.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0376"/>
    <dbReference type="EnsemblBacteria" id="AEF95932">
        <property type="protein sequence ID" value="AEF95932"/>
        <property type="gene ID" value="Metig_0376"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0376"/>
    <dbReference type="HOGENOM" id="CLU_114389_2_0_2"/>
    <dbReference type="OMA" id="AAALWYK"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008792">
        <property type="term" value="F:arginine decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006527">
        <property type="term" value="P:arginine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01404">
        <property type="entry name" value="PvlArgDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016104">
        <property type="entry name" value="Pyr-dep_his/arg-deCO2ase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016105">
        <property type="entry name" value="Pyr-dep_his/arg-deCO2ase_sand"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002724">
        <property type="entry name" value="Pyruvoyl-dep_arg_deCO2ase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40438">
        <property type="entry name" value="PTHR40438"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01862">
        <property type="entry name" value="PvlArgDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005216">
        <property type="entry name" value="Pyruvoyl-dep_arg_deCO2ase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01170">
        <property type="entry name" value="Pyruvoyl-dependent_arginine_de"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56271">
        <property type="entry name" value="SSF56271"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00286">
        <property type="entry name" value="TIGR00286"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="4 6 7" id="KW-0456">Lyase</keyword>
    <keyword evidence="5 6" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_5023332347" description="Pyruvoyl-dependent arginine decarboxylase subunit beta" evidence="6">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5023332348" description="Pyruvoyl-dependent arginine decarboxylase subunit alpha" evidence="6">
        <location>
            <begin position="56"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="site" description="Cleavage (non-hydrolytic)" evidence="6">
        <location>
            <begin position="55"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="modified residue" description="Pyruvic acid (Ser)" evidence="6">
        <location>
            <position position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEF95932.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="168" mass="17990" checksum="08C946077CADB5BE" modified="2011-07-27" version="1">MSALNTDLSCIHAPFRVPNTVSLVAGSAEGKTPLNAFDNALLKAGIGNLNLIRISSIMPPKAEIVPLPKIPMGSLVPTAYGYIISDVKGETIAAAISVAIPKDKELCGLIMEYEGKCSKKEAEKTVREMAKIGFEERGWEIDKIVSIATEHTVENIGCAFAAAALWYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>F6BB67</accession>
    <name>F6BB67_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">S-inosyl-L-homocysteine hydrolase</fullName>
            <shortName evidence="4">SIHH</shortName>
            <ecNumber evidence="4">3.13.1.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Metig_0396</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Catalyzes the hydrolysis of S-inosyl-L-homocysteine (SIH) to L-homocysteine (Hcy) and inosine. Likely functions in a S-adenosyl-L-methionine (SAM) recycling pathway from S-adenosyl-L-homocysteine (SAH) produced from SAM-dependent methylation reactions. Can also catalyze the reverse reaction in vitro, i.e. the synthesis of SIH from Hcy and inosine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>H2O + S-inosyl-L-homocysteine = inosine + L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:59828"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17596"/>
            <dbReference type="ChEBI" id="CHEBI:57985"/>
            <dbReference type="ChEBI" id="CHEBI:58199"/>
            <dbReference type="EC" id="3.13.1.9"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </cofactor>
        <text evidence="4">Binds 1 NAD(+) per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4">Amino-acid biosynthesis; S-adenosyl-L-methionine biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="miscellaneous">
        <text evidence="4">SAH is a product of SAM methyltransferases and is known to be a feedback inhibitor of these enzymes. As a result of this inhibition, organisms have evolved efficient enzymes to metabolize SAH via different pathways. The pathway found in methanogens differs from the canonical pathway, it uses the deamination of S-adenosyl-L-homocysteine to form S-inosyl-L-homocysteine for the regeneration of SAM from S-adenosyl-L-homocysteine.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4 5">Belongs to the adenosylhomocysteinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.13.1.9" evidence="4"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95952.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0396"/>
    <dbReference type="EnsemblBacteria" id="AEF95952">
        <property type="protein sequence ID" value="AEF95952"/>
        <property type="gene ID" value="Metig_0396"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0396"/>
    <dbReference type="HOGENOM" id="CLU_025194_2_1_2"/>
    <dbReference type="OMA" id="NKYGCRE"/>
    <dbReference type="UniPathway" id="UPA00315"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004013">
        <property type="term" value="F:adenosylhomocysteinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006556">
        <property type="term" value="P:S-adenosylmethionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00401">
        <property type="entry name" value="SAHH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1480">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00563">
        <property type="entry name" value="AdoHcyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042172">
        <property type="entry name" value="Adenosylhomocyst_ase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000043">
        <property type="entry name" value="Adenosylhomocysteinase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015878">
        <property type="entry name" value="Ado_hCys_hydrolase_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020082">
        <property type="entry name" value="S-Ado-L-homoCys_hydrolase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23420">
        <property type="entry name" value="PTHR23420"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05221">
        <property type="entry name" value="AdoHcyase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00670">
        <property type="entry name" value="AdoHcyase_NAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001109">
        <property type="entry name" value="Ad_hcy_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00996">
        <property type="entry name" value="AdoHcyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00997">
        <property type="entry name" value="AdoHcyase_NAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00936">
        <property type="entry name" value="ahcY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00738">
        <property type="entry name" value="ADOHCYASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00739">
        <property type="entry name" value="ADOHCYASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 4" id="KW-0520">NAD</keyword>
    <keyword evidence="2 4" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AdoHcyase_NAD" evidence="6">
        <location>
            <begin position="195"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="135"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="160"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="161"/>
            <end position="163"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="190"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="194"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="195"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="224"/>
            <end position="229"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="247"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="303"/>
            <end position="305"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="349"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU004166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEF95952.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="427" mass="47880" checksum="B5001CAA2D43629E" modified="2011-07-27" version="1">MIRSIKDQFGERMYNVKDINLAPEGEKKIEWAKQHMPVLGLIRKEFKKEKPFKGITIGMALHLEAKTAVLAETLMEGGAKIAITGCNPLSTQDDVAAACAKKGMHVYAWRGETTEEYYENLNKVLDHNPDIIIDDGCDLIFLLHTQRTELLDNIMGGCEETTTGIIRLKAMEREGALKFPVMDVNDAYTKYLFDNRYGTGQSAIDGIIRTTNLLIAGKTVVVAGYGWCGKGVAMRAKGMGANVVVTEVNPIRALEAKMDGFRVMKMEDAAKIGDIFITTTGCKDVIRKEHMLLMKDGAILCNAGHFDNEINKRDLRELATSVRVVRNNIEEYKLGDKKLYLLGEGRLVNLACADGHPCEVMDMSFANQALGAKYILENHDKLENKVYRIPYEQDLRIASLKLKSMGVEIDELTEEQKKYLEDWREGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>F6BB70</accession>
    <name>F6BB70_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_0399</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95955.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798564.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF95955">
        <property type="protein sequence ID" value="AEF95955"/>
        <property type="gene ID" value="Metig_0399"/>
    </dbReference>
    <dbReference type="GeneID" id="10643236"/>
    <dbReference type="KEGG" id="mig:Metig_0399"/>
    <dbReference type="HOGENOM" id="CLU_1072045_0_0_2"/>
    <dbReference type="OrthoDB" id="132863at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097367">
        <property type="term" value="F:carbohydrate derivative binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901135">
        <property type="term" value="P:carbohydrate derivative metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046348">
        <property type="entry name" value="SIS_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53697">
        <property type="entry name" value="SSF53697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF95955.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="259" mass="30016" checksum="444A704948BDFF11" modified="2011-07-27" version="1">MEPLSYYVERAVEVLKNIEEPDKETIENLKNRAFIGNGNAFQVAKYYANKLNGYAIEPTYYKSLKKDERVCVISASGSKDSIEICKYFDDVVLITCNKDAPAKEYAKETIVLPSVKEPPFYNVSTYSAMIYWIDRQDYKFREDGFLKYLLRKPSIIFIGDELTYPIACMCALKVREIFGKLSAGLSDMEAYHGWFLHDNDDEAVICLNTNFDLVNNYKIVGTPLELLLTIYYNIGFLQEELEINDYKYDMVLKKKGWKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBA9</accession>
    <name>F6BBA9_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Xylose isomerase domain-containing protein TIM barrel</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1583</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97116.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799709.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1583"/>
    <dbReference type="EnsemblBacteria" id="AEF97116">
        <property type="protein sequence ID" value="AEF97116"/>
        <property type="gene ID" value="Metig_1583"/>
    </dbReference>
    <dbReference type="GeneID" id="10644456"/>
    <dbReference type="KEGG" id="mig:Metig_1583"/>
    <dbReference type="HOGENOM" id="CLU_050006_7_0_2"/>
    <dbReference type="OMA" id="SLFFWEY"/>
    <dbReference type="OrthoDB" id="46548at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001719">
        <property type="entry name" value="AP_endonuc_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036237">
        <property type="entry name" value="Xyl_isomerase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013022">
        <property type="entry name" value="Xyl_isomerase-like_TIM-brl"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01261">
        <property type="entry name" value="AP_endonuc_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00518">
        <property type="entry name" value="AP2Ec"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51658">
        <property type="entry name" value="SSF51658"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0413">Isomerase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AP_endonuc_2" evidence="1">
        <location>
            <begin position="23"/>
            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF97116.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="267" mass="31550" checksum="EDF6B8CB5E6FA84F" modified="2011-07-27" version="1">MSIISCTTLFFWEYPIEEIADIFKSVGLKSMEFFPENPEFWDKRFDEDYLKEIKSILSKFYLTIHAPYIELNPSSTNPYIQEATIKETLWAIDLANFLGAEVLTIHPGKRPTKRPPTMEEYEKFYHYLDVCIPYAEEKSVTLSLENMPKLVNYICHSVEEIKQIIERYRIGLTLDFAHAKENAERFVEELHEYIKNTHISGVCDGREHYPLYHSQIDFSPYIKKLVDYGYKGSLVIEINDLNYNKELSKEEKISELLKEIEYLEKLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBB2</accession>
    <name>F6BBB2_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ribonuclease P protein component 3</fullName>
            <shortName evidence="5">RNase P component 3</shortName>
            <ecNumber evidence="5">3.1.26.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Rpp30</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">rnp3</name>
        <name evidence="6" type="ordered locus">Metig_1586</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Part of ribonuclease P, a protein complex that generates mature tRNA molecules by cleaving their 5'-ends.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>Endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor.</text>
            <dbReference type="EC" id="3.1.26.5"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="5">Consists of a catalytic RNA component and at least 4-5 protein subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the eukaryotic/archaeal RNase P protein component 3 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.5" evidence="5"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97119.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799712.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1586"/>
    <dbReference type="EnsemblBacteria" id="AEF97119">
        <property type="protein sequence ID" value="AEF97119"/>
        <property type="gene ID" value="Metig_1586"/>
    </dbReference>
    <dbReference type="GeneID" id="10644459"/>
    <dbReference type="KEGG" id="mig:Metig_1586"/>
    <dbReference type="HOGENOM" id="CLU_074509_0_0_2"/>
    <dbReference type="OMA" id="INRAACE"/>
    <dbReference type="OrthoDB" id="66325at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030677">
        <property type="term" value="C:ribonuclease P complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004526">
        <property type="term" value="F:ribonuclease P activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001682">
        <property type="term" value="P:tRNA 5'-leader removal"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00756">
        <property type="entry name" value="RNase_P_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016195">
        <property type="entry name" value="Pol/histidinol_Pase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023539">
        <property type="entry name" value="RNase_P_comp-3_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002738">
        <property type="entry name" value="RNase_P_p30"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01876">
        <property type="entry name" value="RNase_P_p30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89550">
        <property type="entry name" value="SSF89550"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 5" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 5" id="KW-0540">Nuclease</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0819">tRNA processing</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEF97119.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="233" mass="27113" checksum="2F72F406CF1902F1" modified="2011-07-27" version="1">MKAVDINRIDNEEGIKRLKKMGWFGAIYTQYHDEFDEEKLNKVIELGEQYGLKIYSGIKIRTQSSKELRNLVRKYRNRFHLVLVEGGVEKVNRSALEMHDVDILSTPELNRKDSGIDHVLARLASVHRVAIELNFNEALTAKNYERARILMAFRRNLKLAKKFDAPVVISSDANDIYTIKSPYDLRAFLNTLVEPEYAKKIIETTYKIAEYRAYLKKKNVVRFGVEIVEDDEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBB6</accession>
    <name>F6BBB6_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_1590</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97123.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1590"/>
    <dbReference type="EnsemblBacteria" id="AEF97123">
        <property type="protein sequence ID" value="AEF97123"/>
        <property type="gene ID" value="Metig_1590"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_1590"/>
    <dbReference type="HOGENOM" id="CLU_155659_4_2_2"/>
    <dbReference type="OMA" id="MNWIEKY"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005651">
        <property type="entry name" value="Trm112-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03966">
        <property type="entry name" value="Trm112p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF97123.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="53" mass="6254" checksum="338350C77C0F13E0" modified="2011-07-27" version="1">MDWIEKYLNILQCPYCGGDLEYIKEKNQLICKSCKKTFDIVDGIPILLKKMKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBC2</accession>
    <name>F6BBC2_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Phosphoribosylformylglycinamidine synthase subunit PurL</fullName>
            <shortName evidence="5">FGAM synthase</shortName>
            <ecNumber evidence="5">6.3.5.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Formylglycinamide ribonucleotide amidotransferase subunit II</fullName>
            <shortName evidence="5">FGAR amidotransferase II</shortName>
            <shortName evidence="5">FGAR-AT II</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">Glutamine amidotransferase PurL</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">Phosphoribosylformylglycinamidine synthase subunit II</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">purL</name>
        <name evidence="9" type="ordered locus">Metig_1596</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Part of the phosphoribosylformylglycinamidine synthase complex involved in the purines biosynthetic pathway. Catalyzes the ATP-dependent conversion of formylglycinamide ribonucleotide (FGAR) and glutamine to yield formylglycinamidine ribonucleotide (FGAM) and glutamate. The FGAM synthase complex is composed of three subunits. PurQ produces an ammonia molecule by converting glutamine to glutamate. PurL transfers the ammonia molecule to FGAR to form FGAM in an ATP-dependent manner. PurS interacts with PurQ and PurL and is thought to assist in the transfer of the ammonia molecule from PurQ to PurL.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>ATP + H2O + L-glutamine + N(2)-formyl-N(1)-(5-phospho-beta-D-ribosyl)glycinamide = 2-formamido-N(1)-(5-O-phospho-beta-D-ribosyl)acetamidine + ADP + H(+) + L-glutamate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:17129"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:147286"/>
            <dbReference type="ChEBI" id="CHEBI:147287"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.5.3"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="5">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Monomer. Part of the FGAM synthase complex composed of 1 PurL, 1 PurQ and 2 PurS subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the FGAMS family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.3" evidence="5"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97129.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799722.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1596"/>
    <dbReference type="EnsemblBacteria" id="AEF97129">
        <property type="protein sequence ID" value="AEF97129"/>
        <property type="gene ID" value="Metig_1596"/>
    </dbReference>
    <dbReference type="GeneID" id="10644469"/>
    <dbReference type="KEGG" id="mig:Metig_1596"/>
    <dbReference type="HOGENOM" id="CLU_003100_0_1_2"/>
    <dbReference type="OMA" id="FIEPYQG"/>
    <dbReference type="OrthoDB" id="860at2157"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00128"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004642">
        <property type="term" value="F:phosphoribosylformylglycinamidine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.650.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00420">
        <property type="entry name" value="PurL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010074">
        <property type="entry name" value="PRibForGlyAmidine_synth_PurL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041609">
        <property type="entry name" value="PurL_linker"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010918">
        <property type="entry name" value="PurM-like_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036676">
        <property type="entry name" value="PurM-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016188">
        <property type="entry name" value="PurM-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036921">
        <property type="entry name" value="PurM-like_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43555">
        <property type="entry name" value="PTHR43555"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00586">
        <property type="entry name" value="AIRS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02769">
        <property type="entry name" value="AIRS_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18072">
        <property type="entry name" value="FGAR-AT_linker"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001587">
        <property type="entry name" value="FGAM_synthase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55326">
        <property type="entry name" value="SSF55326"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56042">
        <property type="entry name" value="SSF56042"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01736">
        <property type="entry name" value="FGAM_synth_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 9" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3 5" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FGAR-AT_linker" evidence="8">
        <location>
            <begin position="2"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="domain" description="AIRS" evidence="6">
        <location>
            <begin position="62"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="domain" description="AIRS_C" evidence="7">
        <location>
            <begin position="191"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="domain" description="AIRS" evidence="6">
        <location>
            <begin position="426"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="domain" description="AIRS_C" evidence="7">
        <location>
            <begin position="562"/>
            <end position="696"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="5">
        <location>
            <begin position="81"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="5">
        <location>
            <begin position="301"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="32"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="5">
        <location>
            <position position="82"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="35"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="80"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="103"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="104"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="229"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="257"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="482"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="519"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="520"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="522"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00420"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF18072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AEF97129.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="739" mass="81385" checksum="DA3E16A2C42D45A3" modified="2011-07-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-06-02" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBD2</accession>
    <name>F6BBD2_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1606</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97139.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799732.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF97139">
        <property type="protein sequence ID" value="AEF97139"/>
        <property type="gene ID" value="Metig_1606"/>
    </dbReference>
    <dbReference type="GeneID" id="10644479"/>
    <dbReference type="KEGG" id="mig:Metig_1606"/>
    <dbReference type="HOGENOM" id="CLU_590024_0_0_2"/>
    <dbReference type="OrthoDB" id="12557at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026453">
        <property type="entry name" value="PGF_pre_PGF"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR04213">
        <property type="entry name" value="PGF_pre_PGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="439"/>
            <end position="459"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF97139.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="463" mass="50475" checksum="5AF1CEB9BD994D35" modified="2011-07-27" version="1">MIKIKNNKSNKTIISLIITMILASMLILSQTAAVSVTLTPSKQECNVGDVIDINAKVEILSSKGEWANIKNITLKVEKDGKVDTVQIPLTLEPGKTVLSNKPLSNTSSEVIEVKLLSTNAVYGYGYGYSYVNDSGYGYLNWHYGYGYGYGYSGYGYNVLNSPSNAIIEYNIKWKPSETGTYKFTLEVTLEDGTKFGSECTVNVNPTTSVTSGGGGGKRTTTSEEGAKSLLEAVPEKYIVKEITVTPGKEVAIAIDKELSDLIGLDKLMITLDKSMNLQVIVSAVKSLPTTVNAPPSGEPFSIFEIVFVNKDAGELVEPKGEVEFKVPKSWLMEKGYGKENVVLERWNGNKWEDISTEVVGEDGEYIKYRAKLNSFCILAICAKEIAVENITNVTVTKNVTTKDVTVNKTKNVTENITAKNVTKVTGNVSKAEEEKKSNIGLVVGIIIILAILGALAYFLTRKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBD5</accession>
    <name>F6BBD5_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Probable [NiFe]-hydrogenase-type-3 Eha complex membrane subunit A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">Metig_1609</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">One of the integral membrane subunits of multisubunit membrane-bound [NiFe]-hydrogenase eha. Eha is predicted to form large electron transfer complex and might catalyze energy-driven reduction of low-potential redox carriers.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Putative multisubunit membrane-bound [NiFe]-hydrogenase eha is composed of at least 20 subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the EhaA family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97142.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799735.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1609"/>
    <dbReference type="EnsemblBacteria" id="AEF97142">
        <property type="protein sequence ID" value="AEF97142"/>
        <property type="gene ID" value="Metig_1609"/>
    </dbReference>
    <dbReference type="GeneID" id="10644482"/>
    <dbReference type="KEGG" id="mig:Metig_1609"/>
    <dbReference type="HOGENOM" id="CLU_174516_1_0_2"/>
    <dbReference type="OMA" id="MKLSFDM"/>
    <dbReference type="OrthoDB" id="118189at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011306">
        <property type="entry name" value="Prd_NiFe_hyd_3_EhaA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17367">
        <property type="entry name" value="NiFe_hyd_3_EhaA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="47"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00020465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00024740"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AEF97142.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="86" mass="9591" checksum="0275296045529B89" modified="2011-07-27" version="1">MVISMEYYVISVIASIIFAFMLKMPIIPKERPIRDSFETSVLFPTPIIALGLTAIDKILFTPDLMSCVLIGLISALFSKFSDKIFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-09-29" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBF6</accession>
    <name>F6BBF6_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Helix-turn-helix type 11 domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1630</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97163.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799755.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF97163">
        <property type="protein sequence ID" value="AEF97163"/>
        <property type="gene ID" value="Metig_1630"/>
    </dbReference>
    <dbReference type="GeneID" id="10644503"/>
    <dbReference type="KEGG" id="mig:Metig_1630"/>
    <dbReference type="HOGENOM" id="CLU_158394_0_0_2"/>
    <dbReference type="OMA" id="SGPFGGY"/>
    <dbReference type="OrthoDB" id="93269at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013196">
        <property type="entry name" value="HTH_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08279">
        <property type="entry name" value="HTH_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH_11" evidence="1">
        <location>
            <begin position="69"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF97163.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="136" mass="15923" checksum="69B8ADE72F0875F5" modified="2011-07-27" version="1">MIESKYVDIEKEIESMVNRKDFDFWEFLKRAYESNVKLDIGHFIILNILIGVGELYRRLSEEVGKNQARKILEKKGIFTKNSEYVSGEYLKKFIGRSSRVAVHNRIRDLKDLGFEIESKSGPLGGYKLVKTPDWFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBM2</accession>
    <name>F6BBM2_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_0475</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96031.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798639.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0475"/>
    <dbReference type="EnsemblBacteria" id="AEF96031">
        <property type="protein sequence ID" value="AEF96031"/>
        <property type="gene ID" value="Metig_0475"/>
    </dbReference>
    <dbReference type="GeneID" id="10643312"/>
    <dbReference type="KEGG" id="mig:Metig_0475"/>
    <dbReference type="HOGENOM" id="CLU_167318_0_0_2"/>
    <dbReference type="OMA" id="PHHIHRL"/>
    <dbReference type="OrthoDB" id="121332at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002753">
        <property type="entry name" value="UPF0058"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036519">
        <property type="entry name" value="UPF0058_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42203">
        <property type="entry name" value="PTHR42203"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01893">
        <property type="entry name" value="UPF0058"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140371">
        <property type="entry name" value="SSF140371"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF96031.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="110" mass="13082" checksum="93B364A4D02D89FF" modified="2011-07-27" version="1">MHKEQLMELHQFFVHVVRELLDDNDNCDSEYLRIYEKLDIKPHYIHKLKVEQRAAIFLLSASIAEILSKNGEKVPENLARRLAENAFKYLNNKRTKSNKGKKLRNSKRNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBM7</accession>
    <name>F6BBM7_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="3">FeoA family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_0480</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96036.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798644.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0480"/>
    <dbReference type="EnsemblBacteria" id="AEF96036">
        <property type="protein sequence ID" value="AEF96036"/>
        <property type="gene ID" value="Metig_0480"/>
    </dbReference>
    <dbReference type="GeneID" id="10643317"/>
    <dbReference type="KEGG" id="mig:Metig_0480"/>
    <dbReference type="HOGENOM" id="CLU_150646_6_3_2"/>
    <dbReference type="OMA" id="NCKIAIG"/>
    <dbReference type="OrthoDB" id="114771at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046914">
        <property type="term" value="F:transition metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007167">
        <property type="entry name" value="Fe-transptr_FeoA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038157">
        <property type="entry name" value="FeoA_core_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008988">
        <property type="entry name" value="Transcriptional_repressor_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04023">
        <property type="entry name" value="FeoA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00899">
        <property type="entry name" value="FeoA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50037">
        <property type="entry name" value="SSF50037"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FeoA" evidence="2">
        <location>
            <begin position="2"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF96036.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="71" mass="7628" checksum="02B5FE3DB73DC4E7" modified="2011-07-27" version="1">METLLDKKPGVYVVKDILGGHGCKQRFYELGICPGKRITLINSGDCGPILVRVGNTKIALGRGMASKIVVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBN1</accession>
    <name>F6BBN1_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Methyl coenzyme M reductase system, component A2</fullName>
            <ecNumber evidence="5">3.6.3.25</ecNumber>
            <ecNumber evidence="5">3.6.3.28</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Metig_0484</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.6.3.25" evidence="5"/>
    <dbReference type="EC" id="3.6.3.28" evidence="5"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96040.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798648.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0484"/>
    <dbReference type="EnsemblBacteria" id="AEF96040">
        <property type="protein sequence ID" value="AEF96040"/>
        <property type="gene ID" value="Metig_0484"/>
    </dbReference>
    <dbReference type="GeneID" id="10643321"/>
    <dbReference type="KEGG" id="mig:Metig_0484"/>
    <dbReference type="HOGENOM" id="CLU_000604_86_2_2"/>
    <dbReference type="OMA" id="AQADKMR"/>
    <dbReference type="OrthoDB" id="25719at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017669">
        <property type="entry name" value="Me_Coenz_M_Rdtase_A2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03269">
        <property type="entry name" value="met_CoM_red_A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="4"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="281"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="36"/>
            <end position="43"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="318"/>
            <end position="325"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEF96040.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="532" mass="59903" checksum="0E75B514B7DA4101" modified="2011-07-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBP2</accession>
    <name>F6BBP2_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PEGA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_0495</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96051.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798659.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96051">
        <property type="protein sequence ID" value="AEF96051"/>
        <property type="gene ID" value="Metig_0495"/>
    </dbReference>
    <dbReference type="GeneID" id="10643332"/>
    <dbReference type="KEGG" id="mig:Metig_0495"/>
    <dbReference type="HOGENOM" id="CLU_028162_0_0_2"/>
    <dbReference type="OMA" id="YPESVFF"/>
    <dbReference type="OrthoDB" id="33001at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013784">
        <property type="entry name" value="Carb-bd-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008969">
        <property type="entry name" value="CarboxyPept-like_regulatory"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013229">
        <property type="entry name" value="PEGA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08308">
        <property type="entry name" value="PEGA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49452">
        <property type="entry name" value="SSF49452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49464">
        <property type="entry name" value="SSF49464"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="662"/>
            <end position="683"/>
        </location>
    </feature>
    <feature type="domain" description="PEGA" evidence="2">
        <location>
            <begin position="205"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF96051.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="685" mass="74760" checksum="02334C85FFB43143" modified="2011-07-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBP3</accession>
    <name>F6BBP3_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0496</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96052.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798660.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0496"/>
    <dbReference type="EnsemblBacteria" id="AEF96052">
        <property type="protein sequence ID" value="AEF96052"/>
        <property type="gene ID" value="Metig_0496"/>
    </dbReference>
    <dbReference type="GeneID" id="10643333"/>
    <dbReference type="KEGG" id="mig:Metig_0496"/>
    <dbReference type="HOGENOM" id="CLU_022168_0_0_2"/>
    <dbReference type="OMA" id="GPEKIYG"/>
    <dbReference type="OrthoDB" id="53086at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.870">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043114">
        <property type="entry name" value="T26-6p_C"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="606"/>
            <end position="624"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96052.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="627" mass="70014" checksum="4662BD6E4786FE13" modified="2011-07-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2020-06-17" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBP7</accession>
    <name>F6BBP7_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_0500</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96056.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96056">
        <property type="protein sequence ID" value="AEF96056"/>
        <property type="gene ID" value="Metig_0500"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0500"/>
    <dbReference type="HOGENOM" id="CLU_217434_0_0_2"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF96056.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="45" mass="5443" checksum="F39EBE3B7BBE174A" modified="2011-07-27" version="1">MAKKVVGRIKRQKGKFYYVDKQGNIVEADRKDMMKNKRKSKKKKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-06-02" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBP8</accession>
    <name>F6BBP8_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_0501</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96057.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798665.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96057">
        <property type="protein sequence ID" value="AEF96057"/>
        <property type="gene ID" value="Metig_0501"/>
    </dbReference>
    <dbReference type="GeneID" id="10643338"/>
    <dbReference type="KEGG" id="mig:Metig_0501"/>
    <dbReference type="HOGENOM" id="CLU_1472097_0_0_2"/>
    <dbReference type="OMA" id="NHTENAS"/>
    <dbReference type="OrthoDB" id="129589at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF96057.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="183" mass="19932" checksum="E0CF7F9E2CAB803E" modified="2011-07-27" version="1">MERNITIGLIIALVLTCIGAFWMSSHSSEGGGFLIDFLQNLTADNSTTNDTYLNDTYTNSTINETINNTDNSTVNETVTDDNSTTLTLSFSTAAVFDNSTNSSIISVDASKENITVGIVLFTSYDNANFTEAASLANVTLPTTITVPVNTSYSEMYYYLEILYNNVTYVIPENTTEVVNISIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBS7</accession>
    <name>F6BBS7_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1675</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97207.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799798.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1675"/>
    <dbReference type="EnsemblBacteria" id="AEF97207">
        <property type="protein sequence ID" value="AEF97207"/>
        <property type="gene ID" value="Metig_1675"/>
    </dbReference>
    <dbReference type="GeneID" id="10644548"/>
    <dbReference type="KEGG" id="mig:Metig_1675"/>
    <dbReference type="HOGENOM" id="CLU_1369593_0_0_2"/>
    <dbReference type="OMA" id="LNRVFEM"/>
    <dbReference type="OrthoDB" id="85299at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="21"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF97207.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="209" mass="24785" checksum="639929CBC7E4ED94" modified="2011-07-27" version="1">MSLWNKIKGKFQKNPIEKLTIRDLEGEKIRLKSKLDRVKKEIKSLDKQKKQLFKEGIGADVLTKKMLAQDIKSIEMEMRLKYKTFLTYQKQFNFVNNLLIVKKYEKELKNIGMWNKITNIQPELLEAKLSEIILDSKEFDSVVEGLNKVFEMRIDEFDEEVDSAEKKLFEAWNQAESGDIEVDEIVENLDLDTKEEDDEELFKRLEKEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-06-02" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBU6</accession>
    <name>F6BBU6_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized conserved protein UCP021940</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_1694</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97226.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF97226">
        <property type="protein sequence ID" value="AEF97226"/>
        <property type="gene ID" value="Metig_1694"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_1694"/>
    <dbReference type="HOGENOM" id="CLU_143941_0_0_2"/>
    <dbReference type="OMA" id="INIAHHV"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014514">
        <property type="entry name" value="UCP021940"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09974">
        <property type="entry name" value="DUF2209"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF97226.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="132" mass="15078" checksum="920F8C86EB61B2C9" modified="2011-07-27" version="1">MLGIDISGRHEVYGKFLRLYAGILAEISADRVIHVEKIEVHYSESTTQTPKDIIKEVKELMSKIKGNYDYIVCEKGEFFRIPKEHVSSLLGKEVIHPKTLGELELINIAHHVSYSCRKLLLKELDLNNGESE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBU7</accession>
    <name>F6BBU7_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="3">CBS domain containing membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_1695</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97227.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799817.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF97227">
        <property type="protein sequence ID" value="AEF97227"/>
        <property type="gene ID" value="Metig_1695"/>
    </dbReference>
    <dbReference type="GeneID" id="10644568"/>
    <dbReference type="KEGG" id="mig:Metig_1695"/>
    <dbReference type="HOGENOM" id="CLU_881698_0_0_2"/>
    <dbReference type="OMA" id="YRHMGYP"/>
    <dbReference type="OrthoDB" id="45353at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="SSF54631"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0129">CBS domain</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBS" evidence="2">
        <location>
            <begin position="58"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="2">
        <location>
            <begin position="121"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF97227.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="321" mass="37276" checksum="B252972B4A3EBCD0" modified="2011-07-27" version="1">MFFKKLSTIERIYNIGLELNHLSEIFEKITKFLIEFEEDIIKTYASSVLNEITVEDIMTKNVLTVPEDMDLEKFEKHIREHKHLGYPVVDKDDNILGIVTFNDLKKIGRELFKKFKVKDVMTPEDKLITVSPKTSAADVQKIMWKNDIGRVLVVDEDNNLLGIVTKSDLLRASVILLNEMPKITECKHITNFYFYDSDMSKVNKLADDMVVLLGARGYAVSEKEGYIEIQTRDWEPLAKIMKSKNRVDHISITTKTLNILDEKIVKEIIDLIEKYSFEEIIITDHITLINERSCIMRIITDITSFRESKKTFGTVTIRIDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBV5</accession>
    <name>F6BBV5_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Cobalt ABC transporter, inner membrane subunit CbiQ</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Metig_1703</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97235.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799825.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1703"/>
    <dbReference type="EnsemblBacteria" id="AEF97235">
        <property type="protein sequence ID" value="AEF97235"/>
        <property type="gene ID" value="Metig_1703"/>
    </dbReference>
    <dbReference type="GeneID" id="10644576"/>
    <dbReference type="KEGG" id="mig:Metig_1703"/>
    <dbReference type="HOGENOM" id="CLU_056469_1_2_2"/>
    <dbReference type="OMA" id="IASFMIR"/>
    <dbReference type="OrthoDB" id="75740at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005887">
        <property type="term" value="C:integral component of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006824">
        <property type="term" value="P:cobalt ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003339">
        <property type="entry name" value="ABC/ECF_trnsptr_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012809">
        <property type="entry name" value="ECF_CbiQ"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02361">
        <property type="entry name" value="CbiQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02454">
        <property type="entry name" value="ECF_T_CbiQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="21"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="44"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="71"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="108"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="148"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="238"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEF97235.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="255" mass="29140" checksum="260E9BCE283A2CE0" modified="2011-07-27" version="1">MHISLSSLERENFKDSIIHKLDARVKLVLTLLIIFYITVYNNVFAMLLVEGYLIFLIILSRLSLLYMFKRIALILPFGGTIALFQPFIKGDTVIYCLFGMPIYQEGLTFGIILFLKVLVSVTGIVLLSSTTPMYEVINAARKLGLPKIMAMLLGLMVRYLFVMYDVLENTIKAQKSRGFNRKNLSYKKILHIFGYTVGNLFLRAYEQGEKTYLAMLSRCYSNESDLFLHKKKIGVEDLAMILITIIVLALGVFLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBW5</accession>
    <name>F6BBW5_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 4">Replication factor C large subunit</fullName>
            <shortName evidence="4">RFC large subunit</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3 4">Clamp loader large subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">rfcL</name>
        <name evidence="7" type="ordered locus">Metig_1713</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Part of the RFC clamp loader complex which loads the PCNA sliding clamp onto DNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Heteromultimer composed of small subunits (RfcS) and large subunits (RfcL).</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the activator 1 small subunits family. RfcL subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97245.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799834.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1713"/>
    <dbReference type="EnsemblBacteria" id="AEF97245">
        <property type="protein sequence ID" value="AEF97245"/>
        <property type="gene ID" value="Metig_1713"/>
    </dbReference>
    <dbReference type="GeneID" id="10644587"/>
    <dbReference type="KEGG" id="mig:Metig_1713"/>
    <dbReference type="HOGENOM" id="CLU_027255_1_1_2"/>
    <dbReference type="OMA" id="GGWTRYG"/>
    <dbReference type="OrthoDB" id="24257at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003689">
        <property type="term" value="F:DNA clamp loader activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01508">
        <property type="entry name" value="RfcL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023935">
        <property type="entry name" value="Rep_factor-C_lsu"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0235">DNA replication</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA" evidence="6">
        <location>
            <begin position="38"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="461"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="461"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="46"/>
            <end position="53"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00014793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00032141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEF97245.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="502" mass="57329" checksum="9D4FAB4976370DBA" modified="2011-07-27" version="1">MLEWVEKYRPKTLKEVAGHNEIKEKLKKWIEDHIKGKNPKPIILVGNPGCGKTTLAHALANDYGFDVIELNASDKRNRAAIRHIVGTAASSKSLTGKNILIILDEVDGISGTEDSGGVSEILKVIKEAKNPIILTANDIYKPTLKPLRDVCEVINVPNVHTNTILAVLKRIAEKEGLDVDEKTLKIIAKHSGGDLRAAINDLEALALGGEISPEIASKLPDRDTERTIFDAMRIIFKTTHYDIATLALMNCNEDLQTVEEWLAENIPNEYKKLEDIERAYDYLSKADIFLGRVYKRQYFGLWKFASALMTAGVALAKDEKYRGFTKYSYPSVLNLLTKTKAKREVLKKILKKIGEKTHASSKRAREDLELLKAIIENNIEMGADLVRYFGITKEELENIVDKKLVSEIFKIIEKKKKEEQKEKKEKKIKEKIVEEKEKKEKIEEKVEEKIIQKEKEETKEIKVEETKEQKEEVKEEKKSKKTKKSEKKEEKAKQLTLDAFFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBX4</accession>
    <name>F6BBX4_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_1722</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97254.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799843.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1722"/>
    <dbReference type="EnsemblBacteria" id="AEF97254">
        <property type="protein sequence ID" value="AEF97254"/>
        <property type="gene ID" value="Metig_1722"/>
    </dbReference>
    <dbReference type="GeneID" id="10644596"/>
    <dbReference type="KEGG" id="mig:Metig_1722"/>
    <dbReference type="HOGENOM" id="CLU_179754_1_0_2"/>
    <dbReference type="OMA" id="VVHSIDM"/>
    <dbReference type="OrthoDB" id="126791at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1340">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003831">
        <property type="entry name" value="DUF211"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023129">
        <property type="entry name" value="MTH889-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42240">
        <property type="entry name" value="PTHR42240"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02680">
        <property type="entry name" value="DUF211"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160363">
        <property type="entry name" value="SSF160363"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF97254.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="97" mass="10906" checksum="30CB4F11432A736E" modified="2011-07-27" version="1">MSGIRRIVLDILKPHEPKITDLALKLCSLKEVDGVNITVYEIDKATENVKITIEGTNLQYDVIKEVIESMSGVIHSIDEVAAGRRIIEDVKTPQDRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F6BC16</accession>
    <name>F6BC16_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">4Fe-4S ferredoxin iron-sulfur binding domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0543</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96097.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798704.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0543"/>
    <dbReference type="EnsemblBacteria" id="AEF96097">
        <property type="protein sequence ID" value="AEF96097"/>
        <property type="gene ID" value="Metig_0543"/>
    </dbReference>
    <dbReference type="GeneID" id="10643381"/>
    <dbReference type="KEGG" id="mig:Metig_0543"/>
    <dbReference type="HOGENOM" id="CLU_1745537_0_0_2"/>
    <dbReference type="OMA" id="VVVKYPY"/>
    <dbReference type="OrthoDB" id="96045at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00037">
        <property type="entry name" value="Fer4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="77"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96097.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="143" mass="17093" checksum="9D7075F76E06C144" modified="2011-07-27" version="1">MEKKKELLKKIREFMILNLEIKKLSKELGINEIHDTYEKVTKLVREPNKKLYKMLYDAAMEIEYEEFGGKKRKEIPWFPKIDYKKCKNCKKCVEFCPKGVYDLENGRVVVKYPYNCIINCNACSYMCCENNAIIFPENKIERL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>F6BC69</accession>
    <name>F6BC69_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ATP-cone domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Metig_1743</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97275.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1743"/>
    <dbReference type="EnsemblBacteria" id="AEF97275">
        <property type="protein sequence ID" value="AEF97275"/>
        <property type="gene ID" value="Metig_1743"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_1743"/>
    <dbReference type="HOGENOM" id="CLU_2534730_0_0_2"/>
    <dbReference type="OMA" id="RIENRIH"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005144">
        <property type="entry name" value="ATP-cone_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03477">
        <property type="entry name" value="ATP-cone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51161">
        <property type="entry name" value="ATP_CONE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-cone" evidence="4">
        <location>
            <begin position="16"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEF97275.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="91" mass="10752" checksum="F591263BA7A6FFB6" modified="2011-07-27" version="1">MQFILVNKMDEVLAKVYITKSDGSKEKFDFEIVKESLRNAGANEELVEEVINRIENRIHDEITTRELKMMITTVLRRLNREVAQKYIQNHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>F6BC71</accession>
    <name>F6BC71_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Oxidoreductase/nitrogenase component 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metig_1745</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the NifD/NifK/NifE/NifN family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97277.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="SMR" id="F6BC71"/>
    <dbReference type="STRING" id="880724.Metig_1745"/>
    <dbReference type="EnsemblBacteria" id="AEF97277">
        <property type="protein sequence ID" value="AEF97277"/>
        <property type="gene ID" value="Metig_1745"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_1745"/>
    <dbReference type="HOGENOM" id="CLU_025876_1_1_2"/>
    <dbReference type="OMA" id="SWDNRGT"/>
    <dbReference type="OrthoDB" id="10827at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016163">
        <property type="term" value="F:nitrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009399">
        <property type="term" value="P:nitrogen fixation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000510">
        <property type="entry name" value="Nase/OxRdtase_comp1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000318">
        <property type="entry name" value="Nase_comp1_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00148">
        <property type="entry name" value="Oxidored_nitro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00699">
        <property type="entry name" value="NITROGENASE_1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 2" id="KW-0535">Nitrogen fixation</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Oxidored_nitro" evidence="3">
        <location>
            <begin position="41"/>
            <end position="432"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU004021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEF97277.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="449" mass="50661" checksum="320B49559775D833" modified="2011-07-27" version="1">MISESQIENFKITPFKITLKKDGLPKCSTRTTPGVITQRSCVYYGARWVLAPIKNTIHVVHGPVGCAYYGAYVRKKNYLTVSSDASEVDIIYGGEKKLFNTIIEAARELKGDAVFVYVTCPTALNGDDVEGICKKAEEILKIPVIPVYCPGFCGYHQSKGHEIGMKALFRLIDLDYENNIDNKKDDDNSKFTVNIIGEFDVADDLKAIKNLLNKLGIRVICTFTGDCNVKSIINSKKAELNLVHCRATGKYIADYMEEKYGIPQLKVSFFGITNTQKSLLDIGKFFGIEDEVVRLINEEYNKIKDELNFYLEKLEGKRVGIFMGGSKIGMLSGAFRDMGMEVVVVGSQFGSLEDYLEAYMNVDENTVLIDDASNIDLQLLMENKKPDIFVGGTKEKYLSLKVGIPFVSFPQDDYPFTGFIGFLNFARELYRNMYHPIWKMIKFNFPTMD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>F6BC73</accession>
    <name>F6BC73_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">THUMP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metig_1747</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97279.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799868.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1747"/>
    <dbReference type="EnsemblBacteria" id="AEF97279">
        <property type="protein sequence ID" value="AEF97279"/>
        <property type="gene ID" value="Metig_1747"/>
    </dbReference>
    <dbReference type="GeneID" id="10644621"/>
    <dbReference type="KEGG" id="mig:Metig_1747"/>
    <dbReference type="HOGENOM" id="CLU_057819_1_0_2"/>
    <dbReference type="OMA" id="FFHPGVM"/>
    <dbReference type="OrthoDB" id="47556at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004809">
        <property type="term" value="F:tRNA (guanine-N2-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002052">
        <property type="entry name" value="DNA_methylase_N6_adenine_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000241">
        <property type="entry name" value="RNA_methylase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004114">
        <property type="entry name" value="THUMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005885">
        <property type="entry name" value="TrmG10"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02926">
        <property type="entry name" value="THUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01170">
        <property type="entry name" value="UPF0020"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00981">
        <property type="entry name" value="THUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01177">
        <property type="entry name" value="TIGR01177"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00092">
        <property type="entry name" value="N6_MTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51165">
        <property type="entry name" value="THUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01261">
        <property type="entry name" value="UPF0020"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="THUMP" evidence="3">
        <location>
            <begin position="46"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEF97279.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="345" mass="39802" checksum="BF97021A2A9FFDD3" modified="2011-07-27" version="1">MKAGYVLSGEHKELPYGELKALFEIFNCKGRVERFDRYVVADGDEELIRKIVKRGGYINEGHLCLFEYDIGEFNEDNIENFVKEFENFANNNELPIDEKDTFAVRVLKLYKTDAINSMDIERKIGHIIKTKTNAKVNLSKPDKLVRVVILDGKIFVGILIEQRDREYFQKNRPHLRKYFHPGCILPKLARAMVNLARVKENDIVLDPFCGTGGFLIEAGLMGCRLIGSDIDWRMASGTLINLESYNLTDKVIAVEKWDAKDVKKFLEKLGIDEVDAIITDPPYGISTSRKGDVENYLKEFKDILKEGGYLVFAAPRKIDIDMDLREIYDVYVHSQLTRYIHVYRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>F6BC81</accession>
    <name>F6BC81_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Acylphosphatase</fullName>
            <ecNumber evidence="1">3.6.1.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metig_1755</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>an acyl phosphate + H2O = a carboxylate + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:14965"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29067"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:59918"/>
            <dbReference type="EC" id="3.6.1.7"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the acylphosphatase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.7" evidence="1"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97287.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799876.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF97287">
        <property type="protein sequence ID" value="AEF97287"/>
        <property type="gene ID" value="Metig_1755"/>
    </dbReference>
    <dbReference type="GeneID" id="10644629"/>
    <dbReference type="KEGG" id="mig:Metig_1755"/>
    <dbReference type="HOGENOM" id="CLU_1656874_0_0_2"/>
    <dbReference type="OrthoDB" id="3289at2182"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003998">
        <property type="term" value="F:acylphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020456">
        <property type="entry name" value="Acylphosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001792">
        <property type="entry name" value="Acylphosphatase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036046">
        <property type="entry name" value="Acylphosphatase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017968">
        <property type="entry name" value="Acylphosphatase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47268">
        <property type="entry name" value="PTHR47268"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00708">
        <property type="entry name" value="Acylphosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54975">
        <property type="entry name" value="SSF54975"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00150">
        <property type="entry name" value="ACYLPHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51160">
        <property type="entry name" value="ACYLPHOSPHATASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acylphosphatase-like" evidence="3">
        <location>
            <begin position="4"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="19"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU004168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEF97287.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="159" mass="18725" checksum="B8251FBA72B5EEC2" modified="2011-07-27" version="1">MTSTYELIIYGKVQHVGFRDRIENLGHALGIDGVIYNHKDGTVRILVNFDDEEIKEMFKKNIKLLEKKDKLITIEKIEERELKTYIEFPEGINRISADDLTELNKKLDEGVRYIKLIFGELKNINKKLDVLFEIKNTQLEMVEMLKQINNTLEKIEKKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>F6BC87</accession>
    <name>F6BC87_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Methyltransferase type 11</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Metig_1761</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97293.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799882.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1761"/>
    <dbReference type="EnsemblBacteria" id="AEF97293">
        <property type="protein sequence ID" value="AEF97293"/>
        <property type="gene ID" value="Metig_1761"/>
    </dbReference>
    <dbReference type="GeneID" id="10644635"/>
    <dbReference type="KEGG" id="mig:Metig_1761"/>
    <dbReference type="HOGENOM" id="CLU_014042_0_0_2"/>
    <dbReference type="OMA" id="DCFCHTG"/>
    <dbReference type="OrthoDB" id="59984at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.130.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002478">
        <property type="entry name" value="PUA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036974">
        <property type="entry name" value="PUA_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041532">
        <property type="entry name" value="RlmI_PUA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019614">
        <property type="entry name" value="SAM-dep_methyl-trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10672">
        <property type="entry name" value="Methyltrans_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17785">
        <property type="entry name" value="PUA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00359">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="SSF88697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 5" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PUA" evidence="4">
        <location>
            <begin position="4"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEF97293.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="387" mass="44247" checksum="483D20F65D34C8DD" modified="2011-07-27" version="1">MDYPKIYVNFAAYSSIEKGNLIVRKHGILNIRDFEKLDVGEIVDVYSKKGRFLGRGFKNPHEIRIVTLKKEKIDENYVRNKIIEADKYRKFLGFKDAYRMVYTQSDWLNGLVIDKYNDIATVQIFNYGFELMKDTIVETLLDLGIDSIYEKSTGRNRRRAGLKEQEGLLCGEKTETIIKEGNAKFKVTFGGQKTGFFLDQRDNRLELEKFIKEGDRVLDVCCYTGGFSVHAALKGAEVVGIDLSKKAVELAKENMQLNGIDDSKYEFRVGNAFEIMEEMIEDGEEFDVVILDPPAFAQSKKDVKNAVRGYHLLNRYGAKLAKRLLVTCSCSHPIEPMEFKAIVIDAALKANKWIRQIGPYRTQPADHPITSKGTEYLKCLFFKVDGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCB3</accession>
    <name>F6BCB3_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Bifunctional IPC transferase and DIPP synthase</fullName>
            <ecNumber evidence="5">2.7.7.74</ecNumber>
            <ecNumber evidence="6">2.7.8.34</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">Metig_1787</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>1D-myo-inositol 3-phosphate + CDP-1L-myo-inositol = bis(1L-myo-inositol) 3,1'-phosphate 1-phosphate + CMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:31327"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58401"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="ChEBI" id="CHEBI:62573"/>
            <dbReference type="ChEBI" id="CHEBI:62576"/>
            <dbReference type="EC" id="2.7.8.34"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>1D-myo-inositol 3-phosphate + CTP + H(+) = CDP-1L-myo-inositol + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:30647"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:58401"/>
            <dbReference type="ChEBI" id="CHEBI:62573"/>
            <dbReference type="EC" id="2.7.7.74"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the CDP-alcohol phosphatidyltransferase class-I family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the C-terminal section; belongs to the CDP-alcohol phosphatidyltransferase class-I family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">In the N-terminal section; belongs to the MobA family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.74" evidence="5"/>
    <dbReference type="EC" id="2.7.8.34" evidence="6"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97319.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799908.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1787"/>
    <dbReference type="EnsemblBacteria" id="AEF97319">
        <property type="protein sequence ID" value="AEF97319"/>
        <property type="gene ID" value="Metig_1787"/>
    </dbReference>
    <dbReference type="GeneID" id="10644664"/>
    <dbReference type="KEGG" id="mig:Metig_1787"/>
    <dbReference type="HOGENOM" id="CLU_643435_0_0_2"/>
    <dbReference type="OMA" id="MVSYSTE"/>
    <dbReference type="OrthoDB" id="115624at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016780">
        <property type="term" value="F:phosphotransferase activity, for other substituted phosphate groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1760">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000462">
        <property type="entry name" value="CDP-OH_P_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043130">
        <property type="entry name" value="CDP-OH_PTrfase_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025877">
        <property type="entry name" value="MobA-like_NTP_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01066">
        <property type="entry name" value="CDP-OH_P_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12804">
        <property type="entry name" value="NTP_transf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00379">
        <property type="entry name" value="CDP_ALCOHOL_P_TRANSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="261"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="385"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="domain" description="NTP_transf_3" evidence="10">
        <location>
            <begin position="3"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00000729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00018322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF12804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AEF97319.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="428" mass="48896" checksum="915AE07EB5CC6A3D" modified="2011-07-27" version="1">MKAVVLAAGLGTRMGKLTEETPKGLLKVAGREIIYRSLKTLENLGVEEFVVITNPKYKKKYEEFLKKEGFNYKIVLNNNPERGNGYSLYLAKGHVNDKFVLVMSDHIYEEGFLKEAIKKEGLIIDKEGKFIDINEATKVLVENGRVKDIGKKLERWHGFDTGLFILTPEIFKYAEEVAKEKELVELSEIVKRAKLKVSEVNGYFWMDVDTEEEMKIAKRLIIQNAIKGSGDGFISRKINRKISTKVSEFLVDYLTPNQITILTFLFGLFSAVVALFNIPLGGILYQISSILDGVDGEIARASMRTSKFGGYVDSILDRYVDFAFLLALAYVIKPSLELWVIIALAIFGSAMVSYSTERYKAAYFKDIYKEIPKMRYLIGKRDERIFVIMIFCLINKIPELFILLAIITNVRVLLTMYLVWRKENKVAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2020-06-17" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCE1</accession>
    <name>F6BCE1_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_0598</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96152.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96152">
        <property type="protein sequence ID" value="AEF96152"/>
        <property type="gene ID" value="Metig_0598"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0598"/>
    <dbReference type="HOGENOM" id="CLU_3020992_0_0_2"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF96152.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="55" mass="6448" checksum="C2FEDE17CFD2B837" modified="2011-07-27" version="1">MKTTVKKNKKKMEEILKTMKIKMIKKITKNMKIIVAIIKAAITKIATLKRKKYLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCF1</accession>
    <name>F6BCF1_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Transposase, IS605 OrfB family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Metig_0609</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96162.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798768.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0609"/>
    <dbReference type="EnsemblBacteria" id="AEF96162">
        <property type="protein sequence ID" value="AEF96162"/>
        <property type="gene ID" value="Metig_0609"/>
    </dbReference>
    <dbReference type="GeneID" id="10643447"/>
    <dbReference type="KEGG" id="mig:Metig_0609"/>
    <dbReference type="HOGENOM" id="CLU_032903_3_4_2"/>
    <dbReference type="OMA" id="MFVDAND"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032196">
        <property type="term" value="P:transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010095">
        <property type="entry name" value="Transposase_IS605_OrfB_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07282">
        <property type="entry name" value="OrfB_Zn_ribbon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01766">
        <property type="entry name" value="tspaseT_teng_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0815">Transposition</keyword>
    <feature type="domain" description="OrfB_Zn_ribbon" evidence="4">
        <location>
            <begin position="317"/>
            <end position="378"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF07282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEF96162.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="426" mass="50491" checksum="5613AD1FF2AAE085" modified="2011-07-27" version="1">MQNKNKLPSEIVLTYKIKHNYDLKNLLDGFMSTSQKAVDIIWENINWKEKQVKHRFKSKNKYKYYTTTRLIPEIPKDKDFKRELRNYLLNGWNFASHYVDGAIKVAYSTIESWKSNYLDGKRKRNKPTFKRPFVRVKNTLIKYDKENGTIRITIKARREYLILDIKNEWFFEKIKGFDIGEIILKDSEALITFKKPLNIFDKKVVIGVDSNLKSLDLFHPEEGWIRIDLSELRRIKNVYDVIIDKLKSIYKKAPKRIGILLKKYYNRRKNRVEDFINKLTSQLSKLFPDAIFIFEDLDKLSMYKNSKFNRDLDRTSWREIAKRLKYKSIVFYVNPHYTSKTCPVCGSKMESQEGQVVKCEKCGVFNRQFVGSFNIFKRGVKLVKKLLGGVGVLPERSDGNLKTVRFLCPWLGWRSMIYSPMNPEES</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCG4</accession>
    <name>F6BCG4_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">V-type proton ATPase subunit E</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">V-ATPase subunit E</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">atpE</name>
        <name evidence="7" type="ordered locus">Metig_0625</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 5">Produces ATP from ADP in the presence of a proton gradient across the membrane.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the V-ATPase E subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96175.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798780.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0625"/>
    <dbReference type="EnsemblBacteria" id="AEF96175">
        <property type="protein sequence ID" value="AEF96175"/>
        <property type="gene ID" value="Metig_0625"/>
    </dbReference>
    <dbReference type="GeneID" id="10643464"/>
    <dbReference type="KEGG" id="mig:Metig_0625"/>
    <dbReference type="HOGENOM" id="CLU_105846_1_0_2"/>
    <dbReference type="OMA" id="YAGNIDC"/>
    <dbReference type="OrthoDB" id="93762at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033178">
        <property type="term" value="C:proton-transporting two-sector ATPase complex, catalytic domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042777">
        <property type="term" value="P:proton motive force-driven plasma membrane ATP synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2320.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00311">
        <property type="entry name" value="ATP_synth_E_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028987">
        <property type="entry name" value="ATP_synth_B-like_membr_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038495">
        <property type="entry name" value="ATPase_E_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002842">
        <property type="entry name" value="ATPase_V1_Esu"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45715">
        <property type="entry name" value="PTHR45715"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01991">
        <property type="entry name" value="vATP-synt_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81573">
        <property type="entry name" value="SSF81573"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0066">ATP synthesis</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="4 5" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0813">Transport</keyword>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="8"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005901"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00311"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEF96175.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="203" mass="22825" checksum="895A713BEFD8D556" modified="2011-07-27" version="1">MGVEKITSKILDDAKKKADEIIKKAEEEANVILKNAEEEAEKRKNAILKKGEKEAEMTRNRIIAEAKLTAKRMILEEKENIIKAAIDKLREDLVKLPEKPEYKEILAKMIKNGAISLGGGELIVQLNERDMGLVEDEVLWKLEKEIEESTGRVTILKKGEPVKIIGGCIISTADGSKILNNSLEAVFERDMENIRAKVTEILF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCN4</accession>
    <name>F6BCN4_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0288 protein Metig_0695</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0695</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0288 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96245.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798848.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0695"/>
    <dbReference type="EnsemblBacteria" id="AEF96245">
        <property type="protein sequence ID" value="AEF96245"/>
        <property type="gene ID" value="Metig_0695"/>
    </dbReference>
    <dbReference type="GeneID" id="10643535"/>
    <dbReference type="KEGG" id="mig:Metig_0695"/>
    <dbReference type="HOGENOM" id="CLU_533841_0_0_2"/>
    <dbReference type="OMA" id="FKKWDVV"/>
    <dbReference type="OrthoDB" id="20383at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01089">
        <property type="entry name" value="UPF0288"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016466">
        <property type="entry name" value="UPF0288_methan"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005852">
        <property type="entry name" value="UCP005852"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03268">
        <property type="entry name" value="methan_mark_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96245.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="499" mass="56451" checksum="C6C2B2E6E52EF3C9" modified="2011-07-27" version="1">MNVIVNGKPKSGETLRDVIKDEPYVDGANIVIIKGSKKEVEEEAKKYVIKTTKGSITIAITENNESSKFWKENYKLFENKNLRWKSGVDVSFGAITIDLDISPEKKQFKKWDVVLSLSGLDKNEGHLVFVKKDVEEMYGLENPKIGIVVGGKRVISNLTKGDKILSIEPIRETKEEVDYLVTKDLNLKLEDGWEIYTYFEATFDGLPKSVEHALAIMEDGFFEISENTNTYIADCRLQTLIIDEENFKDRDRGVITVRNTGNGIGKVYIYREGRVSSLSHTVVGKVTKGMELIDFSEEGILTVVTNPKRLNAIGLTQREAEELFKEYGIKVVREGNTDDDAIVIEQIPEYTMDILKTKEVKVVGIEPDKLVCIEIFDKEAPITAWYFRKTTGLTTRRVGKLKVYFKHKDLIMFEGNKEYAKGLLPENTPKDKVEANTIAVTNMVKRYKGMIGVRLSDSDKFGPTGETFEGTNLVAKIVKNAEYLMNVKNGDTVYLLEIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCQ8</accession>
    <name>F6BCQ8_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Peptidyl-tRNA hydrolase</fullName>
            <shortName evidence="6">PTH</shortName>
            <ecNumber evidence="6">3.1.1.29</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">pth</name>
        <name evidence="7" type="ordered locus">Metig_0719</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 6">The natural substrate for this enzyme may be peptidyl-tRNAs which drop off the ribosome during protein synthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5 6">
            <text>an N-acyl-L-alpha-aminoacyl-tRNA + H2O = a tRNA + an N-acyl-L-amino acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:54448"/>
            <dbReference type="Rhea" id="RHEA-COMP:10123"/>
            <dbReference type="Rhea" id="RHEA-COMP:13883"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:59874"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:138191"/>
            <dbReference type="EC" id="3.1.1.29"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the PTH2 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.29" evidence="6"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96269.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798872.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0719"/>
    <dbReference type="EnsemblBacteria" id="AEF96269">
        <property type="protein sequence ID" value="AEF96269"/>
        <property type="gene ID" value="Metig_0719"/>
    </dbReference>
    <dbReference type="GeneID" id="10643559"/>
    <dbReference type="KEGG" id="mig:Metig_0719"/>
    <dbReference type="HOGENOM" id="CLU_073661_2_2_2"/>
    <dbReference type="OMA" id="GHAAVEC"/>
    <dbReference type="OrthoDB" id="115947at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004045">
        <property type="term" value="F:aminoacyl-tRNA hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02430">
        <property type="entry name" value="PTH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1490.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00628">
        <property type="entry name" value="Pept_tRNA_hydro_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023476">
        <property type="entry name" value="Pep_tRNA_hydro_II_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034759">
        <property type="entry name" value="Pept_tRNA_hydro_arch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002833">
        <property type="entry name" value="PTH2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12649">
        <property type="entry name" value="PTHR12649"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01981">
        <property type="entry name" value="PTH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102462">
        <property type="entry name" value="SSF102462"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00283">
        <property type="entry name" value="arch_pth2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 6 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00033690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00628"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEF96269.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="116" mass="12744" checksum="C484F3FFC061EF3F" modified="2011-07-27" version="1">MYKQAIVIRNDLKMGKGKIAAQACHAAIEAFLDAEKICPNVVKEWLREGQKKVVLKVNSEKELFDIYLTAKSLGLPCSLIKDAGRTQLEPGTTTAVGIGPEKEEKIDKVVGHLKLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCU3</accession>
    <name>F6BCU3_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="5">CDP-diacylglycerol/serine O-phosphatidyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Metig_0758</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 3">Belongs to the CDP-alcohol phosphatidyltransferase class-I family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96304.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798907.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0758"/>
    <dbReference type="EnsemblBacteria" id="AEF96304">
        <property type="protein sequence ID" value="AEF96304"/>
        <property type="gene ID" value="Metig_0758"/>
    </dbReference>
    <dbReference type="GeneID" id="10643597"/>
    <dbReference type="KEGG" id="mig:Metig_0758"/>
    <dbReference type="HOGENOM" id="CLU_049944_3_1_2"/>
    <dbReference type="OMA" id="YSICGML"/>
    <dbReference type="OrthoDB" id="68873at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016780">
        <property type="term" value="F:phosphotransferase activity, for other substituted phosphate groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1760">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004533">
        <property type="entry name" value="CDP-diaglyc--ser_O-PTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000462">
        <property type="entry name" value="CDP-OH_P_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043130">
        <property type="entry name" value="CDP-OH_PTrfase_TM_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01066">
        <property type="entry name" value="CDP-OH_P_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00473">
        <property type="entry name" value="pssA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00379">
        <property type="entry name" value="CDP_ALCOHOL_P_TRANSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="61"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="86"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="110"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="134"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="162"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEF96304.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="200" mass="22085" checksum="FD8EC3800FCD0B17" modified="2011-07-27" version="1">MFSIRKIITISDYVTMANIIFGLLAILLHDFRFIYLSIIFDAMDGFVARKTNTVSDFGAELDSICDVVSFGVAPAYLLYYNFESNFALISALIFALCGALRLARFGILNVKHFVGLPIPAGALVLASFCEVLDNKVLISIIAIIVGLLMISDISYPKYPHKVLIGVFFVSLVLAMWGFVYPVLLCGVGYVLYGIFKTKSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCV3</accession>
    <name>F6BCV3_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="5">PUA domain containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Metig_0768</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">tRNA modification; archaeosine-tRNA biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the archaeosine synthase type 1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96314.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798917.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0768"/>
    <dbReference type="EnsemblBacteria" id="AEF96314">
        <property type="protein sequence ID" value="AEF96314"/>
        <property type="gene ID" value="Metig_0768"/>
    </dbReference>
    <dbReference type="GeneID" id="10643608"/>
    <dbReference type="KEGG" id="mig:Metig_0768"/>
    <dbReference type="HOGENOM" id="CLU_029831_0_0_2"/>
    <dbReference type="OMA" id="YPAAHYD"/>
    <dbReference type="OrthoDB" id="21064at2157"/>
    <dbReference type="UniPathway" id="UPA00393"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016763">
        <property type="term" value="F:pentosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006400">
        <property type="term" value="P:tRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.130.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040777">
        <property type="entry name" value="DUF5591"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002478">
        <property type="entry name" value="PUA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036974">
        <property type="entry name" value="PUA_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036511">
        <property type="entry name" value="TGT-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004521">
        <property type="entry name" value="Uncharacterised_CHP00451"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036895">
        <property type="entry name" value="Uracil-DNA_glycosylase-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17884">
        <property type="entry name" value="DUF5591"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01472">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00359">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51713">
        <property type="entry name" value="SSF51713"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52141">
        <property type="entry name" value="SSF52141"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="SSF88697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00451">
        <property type="entry name" value="unchar_dom_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PUA" evidence="4">
        <location>
            <begin position="471"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="380"/>
            <end position="407"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEF96314.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="539" mass="61957" checksum="21FCC87CD4BE8CCB" modified="2011-07-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-06-02" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCW9</accession>
    <name>F6BCW9_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_0785</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96330.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798933.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96330">
        <property type="protein sequence ID" value="AEF96330"/>
        <property type="gene ID" value="Metig_0785"/>
    </dbReference>
    <dbReference type="GeneID" id="10643625"/>
    <dbReference type="KEGG" id="mig:Metig_0785"/>
    <dbReference type="HOGENOM" id="CLU_1811484_0_0_2"/>
    <dbReference type="OMA" id="AIFTMYW"/>
    <dbReference type="OrthoDB" id="88062at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF96330.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="144" mass="17380" checksum="3414E4224221A362" modified="2011-07-27" version="1">MELEEFITMVEDFLKKEGFLIYPTSDTLENFPEIRWGGDDWKSFIMVAKYEGVKTMFIKKDLFTKEHIESNLYPEKRGDKRIVEYNSKIRSFEKYINKVAVFTMFWIKDDIVYSFTKYALWAEEFLNITLDLGEDNEEEEISWM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCX0</accession>
    <name>F6BCX0_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">NADPH-dependent FMN reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0786</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96331.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798934.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0786"/>
    <dbReference type="EnsemblBacteria" id="AEF96331">
        <property type="protein sequence ID" value="AEF96331"/>
        <property type="gene ID" value="Metig_0786"/>
    </dbReference>
    <dbReference type="GeneID" id="10643626"/>
    <dbReference type="KEGG" id="mig:Metig_0786"/>
    <dbReference type="HOGENOM" id="CLU_050993_3_0_2"/>
    <dbReference type="OMA" id="KFADGNP"/>
    <dbReference type="OrthoDB" id="89591at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029039">
        <property type="entry name" value="Flavoprotein-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005025">
        <property type="entry name" value="FMN_Rdtase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03358">
        <property type="entry name" value="FMN_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52218">
        <property type="entry name" value="SSF52218"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FMN_red" evidence="1">
        <location>
            <begin position="1"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96331.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="195" mass="20932" checksum="0C944A6B2BFEDEEA" modified="2011-07-27" version="1">MKILGISGSPRKEGNTTFLVKEALKAAEEEGVETEFISLAEKEINPCIACNVCKETGECAILDDVDEILQKMKEADGIIIGSPVYFGGVSAQLKMLMDRSRPLRRGFQMSNKVGGAIAVGASRNGGQETTIQQIHNFYFIHSMIVVGDKEPTAHYGGTGVGAAVDDCKNDEDGIETARNLGKKVAEVVKLIKGKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-06-02" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCX4</accession>
    <name>F6BCX4_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_0790</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96335.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798938.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96335">
        <property type="protein sequence ID" value="AEF96335"/>
        <property type="gene ID" value="Metig_0790"/>
    </dbReference>
    <dbReference type="GeneID" id="10643630"/>
    <dbReference type="KEGG" id="mig:Metig_0790"/>
    <dbReference type="HOGENOM" id="CLU_171545_0_0_2"/>
    <dbReference type="OMA" id="QNSDTFK"/>
    <dbReference type="OrthoDB" id="124810at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF96335.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="107" mass="12791" checksum="C7475FFB45A744D6" modified="2011-07-27" version="1">MDIDEVIKLISNAKEENFQKTKHFKIRYKCRNKKLPEESEFINILLTKKPVGILKQGVDKFKVYYELDEEYDMIVILSIKNTNPLKINLITVFPSKRSRRERDDDNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>F6BD06</accession>
    <name>F6BD06_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcriptional regulator, XRE family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0822</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96367.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798969.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0822"/>
    <dbReference type="EnsemblBacteria" id="AEF96367">
        <property type="protein sequence ID" value="AEF96367"/>
        <property type="gene ID" value="Metig_0822"/>
    </dbReference>
    <dbReference type="GeneID" id="10643663"/>
    <dbReference type="KEGG" id="mig:Metig_0822"/>
    <dbReference type="HOGENOM" id="CLU_130237_0_0_2"/>
    <dbReference type="OMA" id="VQCEMCG"/>
    <dbReference type="OrthoDB" id="105095at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004451">
        <property type="entry name" value="CHP00270"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01381">
        <property type="entry name" value="HTH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00530">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00270">
        <property type="entry name" value="TIGR00270"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH cro/C1-type" evidence="1">
        <location>
            <begin position="81"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96367.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="160" mass="18201" checksum="E1AA6451B57D98F0" modified="2011-07-27" version="1">MKICELCGKETTKLYKVRIEGAEMAVCKECAKFGVTPKSYSRLGVAKTSTSKTTSTKKPKRPYRDLFDNLKTLVEDYGDIIREAREKKGLTIEELAKRVGIKVSTLHKIERNELEPEEKYVKRLEKELGISLYEEGDIDYSVSSGDEGLTLGDFIKIKRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>F6BD12</accession>
    <name>F6BD12_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_0828</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96373.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798975.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0828"/>
    <dbReference type="EnsemblBacteria" id="AEF96373">
        <property type="protein sequence ID" value="AEF96373"/>
        <property type="gene ID" value="Metig_0828"/>
    </dbReference>
    <dbReference type="GeneID" id="10643669"/>
    <dbReference type="KEGG" id="mig:Metig_0828"/>
    <dbReference type="HOGENOM" id="CLU_144580_2_0_2"/>
    <dbReference type="OMA" id="IPWEESD"/>
    <dbReference type="OrthoDB" id="103620at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007417">
        <property type="entry name" value="DUF473"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04322">
        <property type="entry name" value="DUF473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF96373.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="123" mass="13451" checksum="979E89376396603B" modified="2011-07-27" version="1">MRIIALAGISPHALSELIKNHIKTLNLKSADNLLALKSVDVGDFVFITSVVKDDIIPGTEGIIGRIRKISIIHQKTPPEVSEERECVVGKVQIEMIGFGTCVDIAEMEVLSPLILDVVMKSIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>F6BD30</accession>
    <name>F6BD30_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein translocase subunit SecE</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Protein transport protein Sec61 gamma subunit homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">secE</name>
        <name evidence="10" type="ordered locus">Metig_0847</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Essential subunit of the Sec protein translocation channel SecYEG. Clamps together the 2 halves of SecY. May contact the channel plug during translocation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the Sec protein translocase complex. Heterotrimer consisting of SecY (alpha), SecG (beta) and SecE (gamma) subunits. The heterotrimers can form oligomers, although 1 heterotrimer is thought to be able to translocate proteins. Interacts with the ribosome. May interact with SecDF, and other proteins may be involved.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cell membrane</location>
            <topology evidence="9">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the SecE/SEC61-gamma family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96391.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798993.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0847"/>
    <dbReference type="EnsemblBacteria" id="AEF96391">
        <property type="protein sequence ID" value="AEF96391"/>
        <property type="gene ID" value="Metig_0847"/>
    </dbReference>
    <dbReference type="GeneID" id="10643688"/>
    <dbReference type="KEGG" id="mig:Metig_0847"/>
    <dbReference type="HOGENOM" id="CLU_191921_0_0_2"/>
    <dbReference type="OMA" id="DFLHQCR"/>
    <dbReference type="OrthoDB" id="121918at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008320">
        <property type="term" value="F:protein transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0065002">
        <property type="term" value="P:intracellular protein transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009306">
        <property type="term" value="P:protein secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006605">
        <property type="term" value="P:protein targeting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00422">
        <property type="entry name" value="SecE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023391">
        <property type="entry name" value="Prot_translocase_SecE_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008158">
        <property type="entry name" value="Translocase_Sec61-g"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001901">
        <property type="entry name" value="Translocase_SecE/Sec61-g"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00584">
        <property type="entry name" value="SecE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103456">
        <property type="entry name" value="SSF103456"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00327">
        <property type="entry name" value="secE_euk_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01067">
        <property type="entry name" value="SECE_SEC61G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 9" id="KW-0811">Translocation</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="42"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AEF96391.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="72" mass="8225" checksum="9E5ACC71FC1C3B53" modified="2011-07-27" version="1">MEKTKNLDMKIEELKNFLLQCKRVLMVTRKPTKEEFINISKVTGLGICILGIIGFVIHVPIVYLKGLIKPVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDB2</accession>
    <name>F6BDB2_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">4Fe-4S ferredoxin iron-sulfur binding domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0930</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96473.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799075.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0930"/>
    <dbReference type="EnsemblBacteria" id="AEF96473">
        <property type="protein sequence ID" value="AEF96473"/>
        <property type="gene ID" value="Metig_0930"/>
    </dbReference>
    <dbReference type="GeneID" id="10643776"/>
    <dbReference type="KEGG" id="mig:Metig_0930"/>
    <dbReference type="HOGENOM" id="CLU_693748_0_0_2"/>
    <dbReference type="OMA" id="ECIGCEK"/>
    <dbReference type="OrthoDB" id="93710at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00037">
        <property type="entry name" value="Fer4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12838">
        <property type="entry name" value="Fer4_7"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="10"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="7"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="59"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="91"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="128"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="157"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="196"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="226"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="265"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="304"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="342"/>
            <end position="371"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96473.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="401" mass="43489" checksum="7DC5CA946844924D" modified="2011-07-27" version="1">MGVLRLGGIEIQQEACLLCNACKEACPTKAIEIAPFITCKICKSCVEACPTGALSDKDGRITYNPTKCLKCGACAKACPTGIKKVDDEFPYSKGHCVLCEKCVDVCPIEIISIPGKVEKPKKEIVIPQEPIAVTDDCVACGVCVDVCPVNAIEIKDNKAVVDESKCIYCGICAQTCPWNAIFVAGKLPNKRKKTIKTFTLDTEKCIGCAKCVDICPGNMIKFDAENLIVKLPKACPACGLCEGVCPVNAITLEVEYEPPKPAYDEGLVWVEENCNYCGSCAMKCPTSAIRVINKRGMELPTKRKTEEKEKFRMCVRCGACTVACPTGALKLGKITIEGKEYNRIEFSPSLCSECGICVDVCPYNVLELTGDDEMPLKGFCVMCLKCVEACKKIKRNALVVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDB5</accession>
    <name>F6BDB5_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Sulfopyruvate decarboxylase, beta subunit</fullName>
            <ecNumber evidence="2">4.1.1.79</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0933</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EC" id="4.1.1.79" evidence="2"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96476.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0933"/>
    <dbReference type="EnsemblBacteria" id="AEF96476">
        <property type="protein sequence ID" value="AEF96476"/>
        <property type="gene ID" value="Metig_0933"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0933"/>
    <dbReference type="HOGENOM" id="CLU_117492_1_0_2"/>
    <dbReference type="OMA" id="GSVLMNM"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050545">
        <property type="term" value="F:sulfopyruvate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006082">
        <property type="term" value="P:organic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901576">
        <property type="term" value="P:organic substance biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044272">
        <property type="term" value="P:sulfur compound biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="CDD" id="cd03372">
        <property type="entry name" value="TPP_ComE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022494">
        <property type="entry name" value="Sulfopyruvate_deCO2ase_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011766">
        <property type="entry name" value="TPP_enzyme-bd_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02775">
        <property type="entry name" value="TPP_enzyme_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="SSF52518"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03846">
        <property type="entry name" value="sulfopy_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0456">Lyase</keyword>
    <keyword evidence="2" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TPP_enzyme_C" evidence="1">
        <location>
            <begin position="40"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96476.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="187" mass="20795" checksum="C042A45BDC2B6FA7" modified="2011-07-27" version="1">MSSMRRIDIIRILMKHVKDELVISNIGFPSKELYHVKDRDRNFYMLGSMGLASSIGLGLALSTNEKVVVIDGDGSVLMNMGSLSTIGYMKPNNLILVIVDNSAYGSTGNQPTHTTNTDLEKVAKACGIDAVTVETEEELEEVFKKALKENKLKVIIAKAIPYNEKVKDIDIPPVVIKYRFMKSFIKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDB9</accession>
    <name>F6BDB9_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="8">MscS Mechanosensitive ion channel</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Metig_0937</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MscS (TC 1.A.23) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96480.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799082.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0937"/>
    <dbReference type="EnsemblBacteria" id="AEF96480">
        <property type="protein sequence ID" value="AEF96480"/>
        <property type="gene ID" value="Metig_0937"/>
    </dbReference>
    <dbReference type="GeneID" id="10643783"/>
    <dbReference type="KEGG" id="mig:Metig_0937"/>
    <dbReference type="HOGENOM" id="CLU_037945_0_1_2"/>
    <dbReference type="OMA" id="NIEFAFP"/>
    <dbReference type="OrthoDB" id="58511at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006685">
        <property type="entry name" value="MscS_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011066">
        <property type="entry name" value="MscS_channel_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011014">
        <property type="entry name" value="MscS_channel_TM-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023408">
        <property type="entry name" value="MscS_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00924">
        <property type="entry name" value="MS_channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="SSF50182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82689">
        <property type="entry name" value="SSF82689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82861">
        <property type="entry name" value="SSF82861"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="15"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="55"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="86"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="140"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEF96480.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="353" mass="39959" checksum="D28205A9E29AE82D" modified="2011-07-27" version="1">MEILNAVYFGNPLKNYLLCILSILFGIFLGRVLHYVIKNYIKAMVSRTKTKLDDIFLYAIDEPLVILAVISGIWIGFSFLELPENISILINEGVVVAVTLCVILFLIKFVDDFILEYVVPLTEKTESKLDDQLIPPLRKFLKILIVVFGFVVILDNLGYDVTTLLAGLGIGGLAVALASKDTLENLIAGILIIVDKPFTIGDWIKVSDYEGIVEEIGIRSTRIRTFGDSLITIPNASLSTNVIENFSAMRKRRVLTTIGITYDTPADKIKKAEEILKDILKNHPAVVEPIRIHFVEYGDWSLNLRVEYFIKNLGFDYYLNALNEINLKIKEEFEKEGIEMAFPTQTVYLRRDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDC1</accession>
    <name>F6BDC1_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_0939</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96482.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799084.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0939"/>
    <dbReference type="EnsemblBacteria" id="AEF96482">
        <property type="protein sequence ID" value="AEF96482"/>
        <property type="gene ID" value="Metig_0939"/>
    </dbReference>
    <dbReference type="GeneID" id="10643785"/>
    <dbReference type="KEGG" id="mig:Metig_0939"/>
    <dbReference type="HOGENOM" id="CLU_1335063_0_0_2"/>
    <dbReference type="OMA" id="HNKYNKD"/>
    <dbReference type="OrthoDB" id="79857at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019202">
        <property type="entry name" value="DUF2067"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09840">
        <property type="entry name" value="DUF2067"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF96482.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="201" mass="23237" checksum="47D753D5FF28B177" modified="2011-07-27" version="1">MRKVITAKTSCSEEMLEICDRISKLNIDCLIESRENLLRIKIFGYDKDSIVENYRTIVSIIERVHRKYTKDEEGMYEYSLSDLKYPVNKDLIIDTLKALGHNVKYLKEEGVIKTSLELDKLNEILCELNEIYGELAFRNIGSKPVKNVIVLAAYLTGKDVDEVIEEGLEKEFFREENGRIVLNKDIKLAKKELAGIEVEKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-06-02" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDD2</accession>
    <name>F6BDD2_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_0950</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96493.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799095.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96493">
        <property type="protein sequence ID" value="AEF96493"/>
        <property type="gene ID" value="Metig_0950"/>
    </dbReference>
    <dbReference type="GeneID" id="10643796"/>
    <dbReference type="KEGG" id="mig:Metig_0950"/>
    <dbReference type="HOGENOM" id="CLU_2056063_0_0_2"/>
    <dbReference type="OrthoDB" id="135151at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF96493.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="119" mass="14147" checksum="BE4BE25B40EFCB6F" modified="2011-07-27" version="1">MIIFVEDRTSEHKVVGKLKPPLSFDMYLTYPQLYGYSTKLTWRYEGDDVYITYFEYVEDDKVYFGKLTIEKDEESCTFTINVDDYGEKSIEILKYALEDCIKAINVLCKNYKLELDTPW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDE0</accession>
    <name>F6BDE0_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Probable Brix domain-containing ribosomal biogenesis protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metig_0958</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Probably involved in the biogenesis of the ribosome.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96501.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0958"/>
    <dbReference type="EnsemblBacteria" id="AEF96501">
        <property type="protein sequence ID" value="AEF96501"/>
        <property type="gene ID" value="Metig_0958"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0958"/>
    <dbReference type="HOGENOM" id="CLU_107897_1_0_2"/>
    <dbReference type="OrthoDB" id="61656at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00699">
        <property type="entry name" value="BriX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007109">
        <property type="entry name" value="Brix"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023548">
        <property type="entry name" value="Brix_dom_Rbsml_bgen_prot"/>
    </dbReference>
    <dbReference type="SMART" id="SM00879">
        <property type="entry name" value="Brix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50833">
        <property type="entry name" value="BRIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="domain" description="Brix" evidence="3">
        <location>
            <begin position="2"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEF96501.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="175" mass="20641" checksum="827B328958991664" modified="2011-07-27" version="1">MNNMIITTSRKPSQRTRSFANDLARALNVEYLTRGKTPLREIFEYYDKVIIIEEFKGNPGKLKIYDVLRNRILSMFISIKLQKEVCGERVTNDNGLAVKYTENTKEYCNLFLDYGFEFVEDSDFVMSFEKVKYRNPPTDKNKMVLFSIQFYKNDRKVGPLIRVKSIKTFDKIDVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDG3</accession>
    <name>F6BDG3_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Methanogenesis marker domain 9</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_0981</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96524.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799126.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0981"/>
    <dbReference type="EnsemblBacteria" id="AEF96524">
        <property type="protein sequence ID" value="AEF96524"/>
        <property type="gene ID" value="Metig_0981"/>
    </dbReference>
    <dbReference type="GeneID" id="10643828"/>
    <dbReference type="KEGG" id="mig:Metig_0981"/>
    <dbReference type="HOGENOM" id="CLU_1727260_0_0_2"/>
    <dbReference type="OMA" id="PCPFRDS"/>
    <dbReference type="OrthoDB" id="96489at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017671">
        <property type="entry name" value="Methan_mark_9"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03277">
        <property type="entry name" value="methan_mark_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF96524.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="155" mass="17866" checksum="14978868DBF52793" modified="2011-07-27" version="1">MWENAPSHICRGGDLRGLAFCCPPIKHCPIHNALAILKMSPEEFIKIKQDFASKTRLGKGKNTCFGSLVWCCKITKPCPFRDGEMRRIKMSADEYMKLKKQLAEEIIRKSKFFEESLKVFEKYGIPKDIAEKCILETGDLKKAYEMAKKIMEEKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDG4</accession>
    <name>F6BDG4_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Xylose isomerase domain-containing protein TIM barrel</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0982</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96525.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0982"/>
    <dbReference type="EnsemblBacteria" id="AEF96525">
        <property type="protein sequence ID" value="AEF96525"/>
        <property type="gene ID" value="Metig_0982"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0982"/>
    <dbReference type="HOGENOM" id="CLU_050006_7_2_2"/>
    <dbReference type="OMA" id="PTIDLNP"/>
    <dbReference type="OrthoDB" id="46548at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036237">
        <property type="entry name" value="Xyl_isomerase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013022">
        <property type="entry name" value="Xyl_isomerase-like_TIM-brl"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01261">
        <property type="entry name" value="AP_endonuc_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51658">
        <property type="entry name" value="SSF51658"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0413">Isomerase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AP_endonuc_2" evidence="1">
        <location>
            <begin position="53"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96525.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="254" mass="29635" checksum="BA0A5EF9E33C6594" modified="2011-07-27" version="1">MVNTMVAKIGVSMSVFLDSEYSLNDALEFLESRVRYVELICDGNMDIMEKFEDLETYNLKYTIHSPITDINLSSHREKVRTMSIEIIEDVLKTSLKVDARLIVVHPGYSIFKHDYERNLNSLINSLKDLNKLKEEYGIDITIENMPSYDMFMFRHPDEYIINNLDEVKITFDIGHSFLNGNIDEFLKIDSIIHTHIHDNNGEFDEHLPIGMGKINFDNFKDSLKNIKGIKMIELQHKSFEYIDDCISKLKNLIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDH9</accession>
    <name>F6BDH9_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Coenzyme F420-dependent N(5),N(10)-methenyltetrahydromethanopterin reductase-related protein</fullName>
            <ecNumber evidence="5">1.12.98.2</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Metig_0998</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the HMD family.</text>
    </comment>
    <dbReference type="EC" id="1.12.98.2" evidence="5"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96540.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799142.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0998"/>
    <dbReference type="EnsemblBacteria" id="AEF96540">
        <property type="protein sequence ID" value="AEF96540"/>
        <property type="gene ID" value="Metig_0998"/>
    </dbReference>
    <dbReference type="GeneID" id="10643845"/>
    <dbReference type="KEGG" id="mig:Metig_0998"/>
    <dbReference type="HOGENOM" id="CLU_069755_0_0_2"/>
    <dbReference type="OMA" id="IMTLQTM"/>
    <dbReference type="OrthoDB" id="49901at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047068">
        <property type="term" value="F:N5,N10-methenyltetrahydromethanopterin hydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006115">
        <property type="entry name" value="6PGDH_NADP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004889">
        <property type="entry name" value="HMD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038182">
        <property type="entry name" value="HMD_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010063">
        <property type="entry name" value="HMDII/III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024190">
        <property type="entry name" value="METHMP_Hmd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03201">
        <property type="entry name" value="HMD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03446">
        <property type="entry name" value="NAD_binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016158">
        <property type="entry name" value="HMD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF500166">
        <property type="entry name" value="HMDII_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="SSF48179"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01724">
        <property type="entry name" value="hmd_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD_binding_2" evidence="4">
        <location>
            <begin position="32"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="domain" description="HMD" evidence="3">
        <location>
            <begin position="194"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="278"/>
            <end position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF03446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEF96540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="341" mass="36804" checksum="AD04E4F8E4B3266B" modified="2011-07-27" version="1">MKVAVYGAGNQELYVNKLRLPETYGGAPPYGGSRMAIEFAKAGHEVYLAEPNRNMLTDEMWKVVEDAGVKVVSDDIEAAKNAEIAILFTPFGKKTFQIAKNILPYLPQNAVIANTCTVSPIVLYSMLEVDLRTKRKDVGITSMHPAAVPGTPQHGHYVISGKATDGTCYATEEQVNKCVELAESAGKKAYVVPADVSSTVADMGSLVTAVALAGVLDYYTVGKKIINAPEKMIEKQIIMTLQTMASLVETSGVKGLLKALNPELLIRSASSMHLLKDQKDLDAALEILSNLNDDLKKEMENAEIKPTTLVAAQSLVKELETLMGGAAAKGAIKRSMRKLFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDI2</accession>
    <name>F6BDI2_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Imidazole glycerol phosphate synthase subunit HisF</fullName>
            <ecNumber evidence="4">4.3.2.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">IGP synthase cyclase subunit</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">IGP synthase subunit HisF</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">ImGP synthase subunit HisF</fullName>
            <shortName evidence="4">IGPS subunit HisF</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">hisF</name>
        <name evidence="6" type="ordered locus">Metig_1001</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The HisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the HisH subunit.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 4">
            <text>5-[(5-phospho-1-deoxy-D-ribulos-1-ylimino)methylamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + L-glutamine = 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate + H(+) + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:24793"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58278"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:58475"/>
            <dbReference type="ChEBI" id="CHEBI:58525"/>
            <dbReference type="EC" id="4.3.2.10"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 4">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 5/9.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Heterodimer of HisH and HisF.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 5">Belongs to the HisA/HisF family.</text>
    </comment>
    <dbReference type="EC" id="4.3.2.10" evidence="4"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96543.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799145.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1001"/>
    <dbReference type="EnsemblBacteria" id="AEF96543">
        <property type="protein sequence ID" value="AEF96543"/>
        <property type="gene ID" value="Metig_1001"/>
    </dbReference>
    <dbReference type="GeneID" id="10643848"/>
    <dbReference type="KEGG" id="mig:Metig_1001"/>
    <dbReference type="HOGENOM" id="CLU_048577_4_0_2"/>
    <dbReference type="OMA" id="IFHYKET"/>
    <dbReference type="OrthoDB" id="57066at2157"/>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00010"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000107">
        <property type="term" value="F:imidazoleglycerol-phosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04731">
        <property type="entry name" value="HisF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01013">
        <property type="entry name" value="HisF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006062">
        <property type="entry name" value="His_biosynth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004651">
        <property type="entry name" value="HisF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00977">
        <property type="entry name" value="His_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="SSF51366"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00735">
        <property type="entry name" value="hisF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 5" id="KW-0368">Histidine biosynthesis</keyword>
    <keyword evidence="6" id="KW-0413">Isomerase</keyword>
    <keyword evidence="3 4" id="KW-0456">Lyase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" evidence="4">
        <location>
            <position position="11"/>
        </location>
    </feature>
    <feature type="active site" evidence="4">
        <location>
            <position position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEF96543.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="274" mass="30466" checksum="F099937CB4C49B9A" modified="2011-07-27" version="1">MLTKRIIPCLDIKDGRVVKGTKFLNLRDAGDPVELSKIYDEQGADEIVFLDITASYEKRDILIDLVERTAEQVFIPLTVGGGVKSVDDFRRILRAGADKISVNTAAVKNPNLVKEAADIFGSQCVVVAIDAKRHYVSEEDLDKEENQDKYIVKTENGYCWFEVYIYGGRKATGIDAVKWAKKVEELGAGEILLTSMDADGTKNGYDLMLTKAISENTKLPVIASGGCGNAQHVYEAFEYGRADAALMASILHYREYTVEEIKLYLKERGIPVRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDI8</accession>
    <name>F6BDI8_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">N-acetyl-gamma-glutamyl-phosphate reductase</fullName>
            <shortName evidence="4">AGPR</shortName>
            <ecNumber evidence="4">1.2.1.38</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">N-acetyl-glutamate semialdehyde dehydrogenase</fullName>
            <shortName evidence="4">NAGSA dehydrogenase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">argC</name>
        <name evidence="7" type="ordered locus">Metig_1007</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Catalyzes the NADPH-dependent reduction of N-acetyl-5-glutamyl phosphate to yield N-acetyl-L-glutamate 5-semialdehyde.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>N-acetyl-L-glutamate 5-semialdehyde + NADP(+) + phosphate = H(+) + N-acetyl-L-glutamyl 5-phosphate + NADPH</text>
            <dbReference type="Rhea" id="RHEA:21588"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29123"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:57936"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.2.1.38"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">Amino-acid biosynthesis; L-arginine biosynthesis; N(2)-acetyl-L-ornithine from L-glutamate: step 3/4.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the NAGSA dehydrogenase family. Type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.38" evidence="4"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96549.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799151.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1007"/>
    <dbReference type="EnsemblBacteria" id="AEF96549">
        <property type="protein sequence ID" value="AEF96549"/>
        <property type="gene ID" value="Metig_1007"/>
    </dbReference>
    <dbReference type="GeneID" id="10643861"/>
    <dbReference type="KEGG" id="mig:Metig_1007"/>
    <dbReference type="HOGENOM" id="CLU_006384_0_1_2"/>
    <dbReference type="OMA" id="PHLTPMI"/>
    <dbReference type="OrthoDB" id="51061at2157"/>
    <dbReference type="UniPathway" id="UPA00068">
        <property type="reaction ID" value="UER00108"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003942">
        <property type="term" value="F:N-acetyl-gamma-glutamyl-phosphate reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006526">
        <property type="term" value="P:arginine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00150">
        <property type="entry name" value="ArgC_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023013">
        <property type="entry name" value="AGPR_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000706">
        <property type="entry name" value="AGPR_type-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000534">
        <property type="entry name" value="Semialdehyde_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012280">
        <property type="entry name" value="Semialdhyde_DH_dimer_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01118">
        <property type="entry name" value="Semialdhyde_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02774">
        <property type="entry name" value="Semialdhyde_dhC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00859">
        <property type="entry name" value="Semialdhyde_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01850">
        <property type="entry name" value="argC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01224">
        <property type="entry name" value="ARGC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="4" id="KW-0055">Arginine biosynthesis</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 4" id="KW-0521">NADP</keyword>
    <keyword evidence="3 4 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Semialdhyde_dh" evidence="6">
        <location>
            <begin position="3"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="active site" evidence="4 5">
        <location>
            <position position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEF96549.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="343" mass="37976" checksum="7167BB6C06EE82F9" modified="2011-07-27" version="1">MITASIIGGTGYTGGELLRLLAGHPHVEVEYVTSRKEAGKKITKIHPHLKGIKKFENLEFMDVDVDKIDSDVVFCATPHGASMKIVPQLIEKGLKVIDLSGDYRFEDIKVYEEWYGLKHIGVLEGVKIAYGLPELHRDEIKDAQLVANPGCFPTGAILAVAPLVKEGIIEERIIIDSKTGVSGAGVNPTETTHFPNVNEDISPYKITTHRHTPEIEKELKKLGNAKVSFTPHLAPLTRGILTTAHTFIREDVKREDIIELYKKFYKNEPFIRIFDDGKVSLTAVRGTNFCDIGGFEIDRNGRLVIISAIDNLVKGASGQAIQNMNIMFGFEETLSLLFGGLRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDJ9</accession>
    <name>F6BDJ9_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="3">AIR synthase related protein domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_1018</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96560.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799162.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1018"/>
    <dbReference type="EnsemblBacteria" id="AEF96560">
        <property type="protein sequence ID" value="AEF96560"/>
        <property type="gene ID" value="Metig_1018"/>
    </dbReference>
    <dbReference type="GeneID" id="10643872"/>
    <dbReference type="KEGG" id="mig:Metig_1018"/>
    <dbReference type="HOGENOM" id="CLU_648312_0_0_2"/>
    <dbReference type="OMA" id="IGGDMVI"/>
    <dbReference type="OrthoDB" id="26664at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009030">
        <property type="term" value="F:thiamine-phosphate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009228">
        <property type="term" value="P:thiamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02691">
        <property type="entry name" value="PurM-like2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.650.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009186">
        <property type="entry name" value="Ni_metllenz_mat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010918">
        <property type="entry name" value="PurM-like_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036676">
        <property type="entry name" value="PurM-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016188">
        <property type="entry name" value="PurM-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036921">
        <property type="entry name" value="PurM-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006283">
        <property type="entry name" value="ThiL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30270">
        <property type="entry name" value="PTHR30270"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00586">
        <property type="entry name" value="AIRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02769">
        <property type="entry name" value="AIRS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006346">
        <property type="entry name" value="Ni_metllenz_mat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55326">
        <property type="entry name" value="SSF55326"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56042">
        <property type="entry name" value="SSF56042"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AIRS" evidence="1">
        <location>
            <begin position="116"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="domain" description="AIRS_C" evidence="2">
        <location>
            <begin position="241"/>
            <end position="388"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF96560.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="450" mass="49845" checksum="839616631C8897D4" modified="2011-07-27" version="1">MDIEGYVRRNLRKNIPEDKIIEDGFRRVMEIKEDVSEEWAKRFIKAVLEEVKTTEKYKEIDDETLKNILEFPKSNVTMGKMGVGSRGEGDFFVHREIARIIESTKQKAEVDSREQDDAGVVKADAKYIVVAVDGTHSRLSEFPFLGGFHVARAALRDVYVMGAEAVALISDVHLADDGDVGKILDFTAGICTVSEAINVPLVAGSTLRVGGDMVLGDRMVSSVGAIGVIKEGKPTARKRAEVGDVILMTEGSGGGTITTTALYYGMFDVIYETLNVDFLTACKSLIESGLVNHVHTMTDVTNGGLRGDAYEISKTANVSLVFDKEVVYKQINPKVLDMLNKLDIDPLGVSIDSLLIIAPEEYADEIRRKAKAEIVGEVREGNESYIIDDGKKIPLVPKFREAAYTPVKKVVGEQKPEDFEEMKERVRKACDEAIKKKDFVLKLLKKKKFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDK3</accession>
    <name>F6BDK3_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Xylose isomerase domain-containing protein TIM barrel</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1022</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96564.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799166.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1022"/>
    <dbReference type="EnsemblBacteria" id="AEF96564">
        <property type="protein sequence ID" value="AEF96564"/>
        <property type="gene ID" value="Metig_1022"/>
    </dbReference>
    <dbReference type="GeneID" id="10643876"/>
    <dbReference type="KEGG" id="mig:Metig_1022"/>
    <dbReference type="HOGENOM" id="CLU_050006_7_2_2"/>
    <dbReference type="OMA" id="HNINIED"/>
    <dbReference type="OrthoDB" id="46548at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001719">
        <property type="entry name" value="AP_endonuc_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036237">
        <property type="entry name" value="Xyl_isomerase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013022">
        <property type="entry name" value="Xyl_isomerase-like_TIM-brl"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01261">
        <property type="entry name" value="AP_endonuc_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00518">
        <property type="entry name" value="AP2Ec"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51658">
        <property type="entry name" value="SSF51658"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0413">Isomerase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AP_endonuc_2" evidence="1">
        <location>
            <begin position="35"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96564.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="269" mass="30438" checksum="BA38AFB71245DE9F" modified="2011-07-27" version="1">MRVGINSQAVKEFNKNGMVWAKDVELIELSLAEVDFINDGNHVIKLAEELSEEYNIRYTIHAPYQDSPLKSTRIKFTEINNKNIKIMERVLELSYKLGAECVVIHGGDVLGKNSIKNVIKNLKEVCNTSKNYGITLALENVFTNEKGIKRAGETPEELLYIVENVGKDTLGINIDIGHAYISSQMHNINIEDYFETLNGHVIHIHAHNNVGLCGEVWDKHLPIFNGKIDYTKLQKMLKTKNIILEVKSGSREEVLDSLKFLSGKKCKKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDL0</accession>
    <name>F6BDL0_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Transcription elongation factor Spt4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">spt4</name>
        <name evidence="4" type="ordered locus">Metig_1029</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Stimulates transcription elongation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Heterodimer composed of Spt4 and Spt5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the archaeal Spt4 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96571.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799173.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1029"/>
    <dbReference type="EnsemblBacteria" id="AEF96571">
        <property type="protein sequence ID" value="AEF96571"/>
        <property type="gene ID" value="Metig_1029"/>
    </dbReference>
    <dbReference type="GeneID" id="10643883"/>
    <dbReference type="KEGG" id="mig:Metig_1029"/>
    <dbReference type="HOGENOM" id="CLU_199467_0_0_2"/>
    <dbReference type="OMA" id="VCRECHR"/>
    <dbReference type="OrthoDB" id="119723at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.28.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00949">
        <property type="entry name" value="Spt4_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029040">
        <property type="entry name" value="RPABC4/Spt4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022800">
        <property type="entry name" value="Spt4/RpoE2_Znf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007178">
        <property type="entry name" value="Spt4_arch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038589">
        <property type="entry name" value="Spt4_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40704">
        <property type="entry name" value="PTHR40704"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06093">
        <property type="entry name" value="Spt4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01389">
        <property type="entry name" value="Spt4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63393">
        <property type="entry name" value="SSF63393"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Spt4" evidence="3">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="4"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="7"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="16"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="19"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEF96571.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="58" mass="6557" checksum="AFBD4C702125C463" modified="2011-07-27" version="1">MRACLKCKYLTNEERCPLCMGETTENWRGLLIVLDPLKSEIAKKAKIDVKGKFALSAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-06-02" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDM9</accession>
    <name>F6BDM9_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1049</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96590.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799191.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96590">
        <property type="protein sequence ID" value="AEF96590"/>
        <property type="gene ID" value="Metig_1049"/>
    </dbReference>
    <dbReference type="GeneID" id="10643903"/>
    <dbReference type="KEGG" id="mig:Metig_1049"/>
    <dbReference type="HOGENOM" id="CLU_2230420_0_0_2"/>
    <dbReference type="OMA" id="LILMIYH"/>
    <dbReference type="OrthoDB" id="131324at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="44"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="79"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96590.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="105" mass="11871" checksum="9B156342CC1F46D6" modified="2011-07-27" version="1">MNVIITIIIISFSILLSVKLYSSLMKRLKKEEIKLKNFEVIYKFVIPIIFALVGVEYSNSIFISAGINPKSFTLSILDTIVVILILMIYHVSLAIISRIIVKISP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDV8</accession>
    <name>F6BDV8_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 12">5,10-methenyltetrahydromethanopterin hydrogenase</fullName>
            <ecNumber evidence="4 12">1.12.98.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10 12">H(2)-dependent methylene-H(4)MPT dehydrogenase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9 12">H(2)-forming N(5),N(10)-methylenetetrahydromethanopterin dehydrogenase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11 12">N(5),N(10)-methenyltetrahydromethanopterin hydrogenase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">hmd</name>
        <name evidence="14" type="ordered locus">Metig_1130</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 12">Catalyzes the reversible reduction of methenyl-H(4)MPT(+) to methylene-H(4)MPT.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>5,10-methenyl-5,6,7,8-tetrahydromethanopterin + H2 = 5,10-methylenetetrahydromethanopterin + H(+)</text>
            <dbReference type="Rhea" id="RHEA:20017"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:18276"/>
            <dbReference type="ChEBI" id="CHEBI:57818"/>
            <dbReference type="ChEBI" id="CHEBI:58337"/>
            <dbReference type="EC" id="1.12.98.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 12">One-carbon metabolism; methanogenesis from CO(2); 5,10-methylene-5,6,7,8-tetrahydromethanopterin from 5,10-methenyl-5,6,7,8-tetrahydromethanopterin (hydrogen route): step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the HMD family.</text>
    </comment>
    <dbReference type="EC" id="1.12.98.2" evidence="4 12"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96669.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799270.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1130"/>
    <dbReference type="EnsemblBacteria" id="AEF96669">
        <property type="protein sequence ID" value="AEF96669"/>
        <property type="gene ID" value="Metig_1130"/>
    </dbReference>
    <dbReference type="GeneID" id="10643988"/>
    <dbReference type="KEGG" id="mig:Metig_1130"/>
    <dbReference type="HOGENOM" id="CLU_772960_0_0_2"/>
    <dbReference type="OMA" id="HACTIPT"/>
    <dbReference type="OrthoDB" id="31540at2157"/>
    <dbReference type="UniPathway" id="UPA00640">
        <property type="reaction ID" value="UER00696"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047068">
        <property type="term" value="F:N5,N10-methenyltetrahydromethanopterin hydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019386">
        <property type="term" value="P:methanogenesis, from carbon dioxide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01090">
        <property type="entry name" value="HMD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010062">
        <property type="entry name" value="HMD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004889">
        <property type="entry name" value="HMD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038182">
        <property type="entry name" value="HMD_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024190">
        <property type="entry name" value="METHMP_Hmd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03201">
        <property type="entry name" value="HMD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016158">
        <property type="entry name" value="HMD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF500165">
        <property type="entry name" value="HMDI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="SSF48179"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01723">
        <property type="entry name" value="hmd_TIGR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 12" id="KW-0484">Methanogenesis</keyword>
    <keyword evidence="6 12" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="8 12 14" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HMD" evidence="13">
        <location>
            <begin position="242"/>
            <end position="338"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00017649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00029901"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00030515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00030763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF03201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="AEF96669.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="358" mass="38610" checksum="C2D296AC53B459E6" modified="2011-07-27" version="1">MKIAILGAGCYRTHAAAGITNFARACEVAEQVGKPEIALTHSTITMGAELLHLCDEVKEVVVSDPCFAEEPGLVVIDEFDPKEVIEAHKEDPEKIMPKIREAVKAKAKELPKPPKACIHLVHPEDVGLKITTDDKEAVADADIILTWLPKGNKQPDIIKKFVDAIPEGAIVTHACTIPTTKFAKIFKDLGRDDLNIISYHPGAVPEMKGQVFLSTGFASDEAVEKLYALAQKARGTAYKLPANLISPVCDMGSAVTATVYAGLLAYRDAVTKILGAPADFAQMMAEEAITQLLELMKKEGIANMEKVLNPKALTGTADSMCFGPLADILPQALEVLKKHGVEDEGEKTKCEIMSQANK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDW3</accession>
    <name>F6BDW3_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Type II secretion system F domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Metig_1135</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96674.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799275.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1135"/>
    <dbReference type="EnsemblBacteria" id="AEF96674">
        <property type="protein sequence ID" value="AEF96674"/>
        <property type="gene ID" value="Metig_1135"/>
    </dbReference>
    <dbReference type="GeneID" id="10643993"/>
    <dbReference type="KEGG" id="mig:Metig_1135"/>
    <dbReference type="HOGENOM" id="CLU_827975_0_0_2"/>
    <dbReference type="OMA" id="DYQYAGI"/>
    <dbReference type="OrthoDB" id="74259at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018076">
        <property type="entry name" value="T2SS_GspF_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00482">
        <property type="entry name" value="T2SSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="116"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="143"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="291"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="324"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="domain" description="T2SSF" evidence="8">
        <location>
            <begin position="183"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00482"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AEF96674.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="349" mass="39681" checksum="B5959F32C634D223" modified="2011-07-27" version="1">MVEISVENIKKFFKKISKRIDDFLIKIGLKRKRIRFRTAWEEYLRRMSSGEEALTFYEPFIEDTPMDVRDIDDLFGDDLGIFEKYSKSLSYWITHTSFLPSKRDFQYIGISDEKKYFAKVLLASLGTFILLFLYGAILNNNPISGILNGFIFAGGLMIAGIFYPKLKLILFRGEIKIQILFALLYMVSVVRVGISLQEAIENVAKSPEYGIVSYEFKAIIHDVNVGGYNFVEALERAKMRTKIPLMEKLYDQLLVAFNKGNVSLLLEKLYEDIVRQSLAKIDSSKFMISNLGNLVFGVGLILPFAGMIQSALGSQQGFPGIIDTVDLILTKIGPISTIIFTIFIKMKIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>F6BE39</accession>
    <name>F6BE39_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">50S ribosomal protein L37e</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">rpl37e</name>
        <name evidence="12" type="ordered locus">Metig_0013</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 10 11">Binds to the 23S rRNA.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="10">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 10 11">Belongs to the eukaryotic ribosomal protein eL37 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95575.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798185.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0013"/>
    <dbReference type="EnsemblBacteria" id="AEF95575">
        <property type="protein sequence ID" value="AEF95575"/>
        <property type="gene ID" value="Metig_0013"/>
    </dbReference>
    <dbReference type="GeneID" id="10642848"/>
    <dbReference type="KEGG" id="mig:Metig_0013"/>
    <dbReference type="HOGENOM" id="CLU_208825_0_0_2"/>
    <dbReference type="OMA" id="RIRRYSW"/>
    <dbReference type="OrthoDB" id="124429at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00547">
        <property type="entry name" value="Ribosomal_L37e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011331">
        <property type="entry name" value="Ribosomal_L37ae/L37e"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001569">
        <property type="entry name" value="Ribosomal_L37e"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018267">
        <property type="entry name" value="Ribosomal_L37e_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011332">
        <property type="entry name" value="Ribosomal_zn-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01907">
        <property type="entry name" value="Ribosomal_L37e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57829">
        <property type="entry name" value="SSF57829"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01077">
        <property type="entry name" value="RIBOSOMAL_L37E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 10 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 10 11" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="8 10 11" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="7 10 11" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4 10 11" id="KW-0699">rRNA-binding</keyword>
    <keyword evidence="6 10 11" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="zinc finger region" description="C4-type" evidence="10">
        <location>
            <begin position="20"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="20"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="23"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="35"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="38"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AEF95575.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="62" mass="7383" checksum="FA6F6B82BA27DE61" modified="2011-07-27" version="1">MSKGTPSQGRKNKGSYHIRCRRCGRRAYHIRKKVCAACGFGRSKRIRRYSWQWKKVNGQRLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>F6BE44</accession>
    <name>F6BE44_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Digeranylgeranylglycerophospholipid reductase</fullName>
            <shortName evidence="8">DGGGPL reductase</shortName>
            <ecNumber evidence="8">1.3.-.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">2,3-bis-O-geranylgeranylglyceryl phosphate reductase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Geranylgeranyl reductase</fullName>
            <shortName evidence="8">GGR</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Metig_0018</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Is involved in the reduction of 2,3-digeranylgeranylglycerophospholipids (unsaturated archaeols) into 2,3-diphytanylglycerophospholipids (saturated archaeols) in the biosynthesis of archaeal membrane lipids. Catalyzes the formation of archaetidic acid (2,3-di-O-phytanyl-sn-glyceryl phosphate) from 2,3-di-O-geranylgeranylglyceryl phosphate (DGGGP) via the hydrogenation of each double bond of the isoprenoid chains.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>2,3-bis-O-(phytanyl)-sn-glycerol 1-phosphate + 8 A = 2,3-bis-O-(geranylgeranyl)-sn-glycerol 1-phosphate + 8 AH2</text>
            <dbReference type="Rhea" id="RHEA:64368"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:58837"/>
            <dbReference type="ChEBI" id="CHEBI:73125"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>8 A + a 2,3-bis-O-phytanyl-sn-glycerol 1-phospholipid = a 2,3-bis-O-(geranylgeranyl)-sn-glycerol 1-phospholipid + 8 AH2</text>
            <dbReference type="Rhea" id="RHEA:64376"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:138139"/>
            <dbReference type="ChEBI" id="CHEBI:138140"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
        <text evidence="8">Binds 1 FAD per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="8">Membrane lipid metabolism; glycerophospholipid metabolism.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="8">Reduction reaction proceeds via syn addition of hydrogen for double bonds.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the geranylgeranyl reductase family. DGGGPL reductase subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.3.-.-" evidence="8"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95580.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798190.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0018"/>
    <dbReference type="EnsemblBacteria" id="AEF95580">
        <property type="protein sequence ID" value="AEF95580"/>
        <property type="gene ID" value="Metig_0018"/>
    </dbReference>
    <dbReference type="GeneID" id="10642853"/>
    <dbReference type="KEGG" id="mig:Metig_0018"/>
    <dbReference type="HOGENOM" id="CLU_024648_0_0_2"/>
    <dbReference type="OMA" id="GMCRREV"/>
    <dbReference type="OrthoDB" id="31233at2157"/>
    <dbReference type="UniPathway" id="UPA00940"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045550">
        <property type="term" value="F:geranylgeranyl reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016628">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-CH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006650">
        <property type="term" value="P:glycerophospholipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046467">
        <property type="term" value="P:membrane lipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01287">
        <property type="entry name" value="DGGGPL_reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023590">
        <property type="entry name" value="DGGGPL_reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002938">
        <property type="entry name" value="FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011777">
        <property type="entry name" value="Geranylgeranyl_Rdtase_fam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01494">
        <property type="entry name" value="FAD_binding_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02032">
        <property type="entry name" value="GG-red-SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0274">FAD</keyword>
    <keyword evidence="2 8" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="1 8" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="5 8" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="4 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6 8" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="7 8" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD_binding_3" evidence="9">
        <location>
            <begin position="8"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="8">
        <location>
            <begin position="206"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="8">
        <location>
            <begin position="287"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="14"/>
            <end position="18"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="37"/>
            <end position="39"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="48"/>
            <end position="51"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="98"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="279"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="291"/>
            <end position="292"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AEF95580.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="391" mass="42792" checksum="70E5FB97583AC5A0" modified="2011-07-27" version="1">MRELMDSYDVVVVGAGPGGSMSAYASAKNGAKTLLIEKSQEIGEPVRCAEAIPSLDDFGIKPSPEFVRNEIKGGFLFAPNGKRVTVTQGKATGYVVERKIFDKFLAIRAAKKGAKIAIKSRVVGLEEEGDGWNVIVKHLDEEYAVKTKIVIAADGVESNVAEYAGLKAKKNPLDICSCAEYEMTNVELLDKNMMEFYFGNDIAPGGYLWIFPKGDTANVGLGVRGSKKKAIDYLNEFLENGLAKDRLKNATPVEFKVGGAPVGGPIEKTYDDGILVVGDAAGQISPLTGGGIYLAMDCGLIAGEVAGKAIKNNDWSEKVLSEYERRWKEKHYECLMSHLKYRKILEKMSDDELNALAEALGDNLDDIDLKKFVKRILTKKPSLIKYFKDLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-06-02" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>F6BE51</accession>
    <name>F6BE51_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0026</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95587.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798197.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF95587">
        <property type="protein sequence ID" value="AEF95587"/>
        <property type="gene ID" value="Metig_0026"/>
    </dbReference>
    <dbReference type="GeneID" id="10642861"/>
    <dbReference type="KEGG" id="mig:Metig_0026"/>
    <dbReference type="HOGENOM" id="CLU_2597739_0_0_2"/>
    <dbReference type="OrthoDB" id="144290at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="43"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF95587.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="79" mass="8949" checksum="FB16FCAE9D408DFF" modified="2011-07-27" version="1">MEKLTESQINELKLSFLLSTVVCSIVISIDILTLIHLIRNVEILRVILHSSGIGILIGYLTFKILKMNLFDYNKNECGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEB5</accession>
    <name>F6BEB5_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Helicase domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_1255</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96792.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1255"/>
    <dbReference type="EnsemblBacteria" id="AEF96792">
        <property type="protein sequence ID" value="AEF96792"/>
        <property type="gene ID" value="Metig_1255"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_1255"/>
    <dbReference type="HOGENOM" id="CLU_1096715_0_0_2"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0347">Helicase</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF96792.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="253" mass="30160" checksum="667ACFF863EDB127" modified="2011-07-27" version="1">MGLEEDFYYLSTSIYPKERLKRIESINKNKNRKIIVSTQLIEAGVDISVDVIYRDIAPLDCINQTAGRCNRHNEGKKGIVNVVKLVDENGRRFADYIYEKHLITKTEELLNEFDVIDEKMFLKLNNKYFKKLSNYKDKSEDILKTIEKFEYNVVEGKFKLIENIPSIDLFVCIEDEAEKVWEEFERINEIKDVFERRREFLKIKRKFYSYVISVPEYAIKGKDILFEHLSKIDKKYYDKETGFEIVEDKTIIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEE1</accession>
    <name>F6BEE1_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="9">ATPase, P-type (Transporting), HAD superfamily, subfamily IC</fullName>
            <ecNumber evidence="9">3.6.3.8</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Metig_0041</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.6.3.8" evidence="9"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95602.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0041"/>
    <dbReference type="EnsemblBacteria" id="AEF95602">
        <property type="protein sequence ID" value="AEF95602"/>
        <property type="gene ID" value="Metig_0041"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0041"/>
    <dbReference type="HOGENOM" id="CLU_002360_3_3_2"/>
    <dbReference type="OMA" id="PLWNNMM"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006068">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004014">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00689">
        <property type="entry name" value="Cation_ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00690">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00831">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="SSF56784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="SSF81653"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="SSF81660"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="SSF81665"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-1278">Translocase</keyword>
    <keyword evidence="1 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="37"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="66"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="216"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="688"/>
            <end position="712"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="753"/>
            <end position="772"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="784"/>
            <end position="801"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="813"/>
            <end position="838"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="850"/>
            <end position="874"/>
        </location>
    </feature>
    <feature type="domain" description="Cation_ATPase_N" evidence="8">
        <location>
            <begin position="5"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AEF95602.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="878" mass="97590" checksum="65F9C77EFAA13229" modified="2011-07-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEF0</accession>
    <name>F6BEF0_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">3-isopropylmalate dehydratase small subunit</fullName>
            <ecNumber evidence="2">4.2.1.33</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">Alpha-IPM isomerase</fullName>
            <shortName evidence="2">IPMI</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="2">Isopropylmalate isomerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2" type="primary">leuD</name>
        <name evidence="4" type="ordered locus">Metig_0050</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>(2R,3S)-3-isopropylmalate = (2S)-2-isopropylmalate</text>
            <dbReference type="Rhea" id="RHEA:32287"/>
            <dbReference type="ChEBI" id="CHEBI:1178"/>
            <dbReference type="ChEBI" id="CHEBI:35121"/>
            <dbReference type="EC" id="4.2.1.33"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 2/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Heterodimer of LeuC and LeuD.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the LeuD family. LeuD type 2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.33" evidence="2"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95611.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798220.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0050"/>
    <dbReference type="EnsemblBacteria" id="AEF95611">
        <property type="protein sequence ID" value="AEF95611"/>
        <property type="gene ID" value="Metig_0050"/>
    </dbReference>
    <dbReference type="GeneID" id="10642885"/>
    <dbReference type="KEGG" id="mig:Metig_0050"/>
    <dbReference type="HOGENOM" id="CLU_081378_1_1_2"/>
    <dbReference type="OMA" id="ITPGRYN"/>
    <dbReference type="OrthoDB" id="86621at2157"/>
    <dbReference type="UniPathway" id="UPA00048">
        <property type="reaction ID" value="UER00071"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003861">
        <property type="term" value="F:3-isopropylmalate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009098">
        <property type="term" value="P:leucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01577">
        <property type="entry name" value="IPMI_Swivel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.19.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01032">
        <property type="entry name" value="LeuD_type2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015928">
        <property type="entry name" value="Aconitase/3IPM_dehydase_swvl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000573">
        <property type="entry name" value="AconitaseA/IPMdHydase_ssu_swvl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033940">
        <property type="entry name" value="IPMI_Swivel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011827">
        <property type="entry name" value="LeuD_type2/HacB/DmdB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00694">
        <property type="entry name" value="Aconitase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02087">
        <property type="entry name" value="LEUD_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="2" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="2" id="KW-0432">Leucine biosynthesis</keyword>
    <keyword evidence="1 2 4" id="KW-0456">Lyase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aconitase_C" evidence="3">
        <location>
            <begin position="43"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEF95611.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="169" mass="18697" checksum="9F64AC4550701820" modified="2011-07-27" version="1">MYIRGKAHVFGNDVDTDAIIPGPYLKTMDGHELASHCMAGIDEDFPKKVKEGDVIVAGENFGCGSSREQAPIAIKYCGIKAVIAESFARIFYRNAINIGLIPIVCKGITKHVKDGDVIEVDLENEKIIINDKITLNCETPKGIEREILEAGGLINYYHRKVKQRCYLDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEF1</accession>
    <name>F6BEF1_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Beta-lactamase domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0051</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95612.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798221.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0051"/>
    <dbReference type="EnsemblBacteria" id="AEF95612">
        <property type="protein sequence ID" value="AEF95612"/>
        <property type="gene ID" value="Metig_0051"/>
    </dbReference>
    <dbReference type="GeneID" id="10642886"/>
    <dbReference type="KEGG" id="mig:Metig_0051"/>
    <dbReference type="HOGENOM" id="CLU_030571_2_6_2"/>
    <dbReference type="OMA" id="KNIVTGH"/>
    <dbReference type="OrthoDB" id="103066at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="SSF56281"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Lactamase_B" evidence="1">
        <location>
            <begin position="21"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF95612.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="207" mass="23336" checksum="C112587B4ACF74B8" modified="2011-07-27" version="1">MIKLLYEGELVRENGVIKKASSSVTLIQTKNHNIIVDTSTRDKRELIIEELKKLNLEPKDIDVIINTHRHYDHIENNDLFKNAVIYASPQECVKECRGCAIYSTTDEVHDFEPIEKFDDDEVIIIKTPGHTWGSISVVYEDYVVAGDAVPLKGNILGNMPPAVRVDNRAAKGSLRRIKLLRKNIITGHDGIVYVNEIPSDNANGELF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEJ1</accession>
    <name>F6BEJ1_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_0091</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95652.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798261.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF95652">
        <property type="protein sequence ID" value="AEF95652"/>
        <property type="gene ID" value="Metig_0091"/>
    </dbReference>
    <dbReference type="GeneID" id="10642926"/>
    <dbReference type="KEGG" id="mig:Metig_0091"/>
    <dbReference type="HOGENOM" id="CLU_1976528_0_0_2"/>
    <dbReference type="OMA" id="QATIHCL"/>
    <dbReference type="OrthoDB" id="116043at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF95652.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="114" mass="13620" checksum="93D5347EEEAA4627" modified="2011-07-27" version="1">MELKELMEKIISNKIKLSLMCRFKSIEQYKNELYNDIAVSQMEDVEALYEKYLMYIGEKPNIKVELEGDIKEILKETIELEKKLIKESGMTFGIRQTTIHCLTSDERFYHYLKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEJ7</accession>
    <name>F6BEJ7_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">4Fe-4S ferredoxin iron-sulfur binding domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0097</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95658.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798267.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0097"/>
    <dbReference type="EnsemblBacteria" id="AEF95658">
        <property type="protein sequence ID" value="AEF95658"/>
        <property type="gene ID" value="Metig_0097"/>
    </dbReference>
    <dbReference type="GeneID" id="10642932"/>
    <dbReference type="KEGG" id="mig:Metig_0097"/>
    <dbReference type="HOGENOM" id="CLU_139698_5_5_2"/>
    <dbReference type="OMA" id="ACPAKIL"/>
    <dbReference type="OrthoDB" id="111853at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12838">
        <property type="entry name" value="Fer4_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="32"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF95658.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="61" mass="6590" checksum="C63247DF02179135" modified="2011-07-27" version="1">MKVTINYDLCKGPECAECVNACPMEVFEIQGDKVVVAREEDCSGCGVCMDVCPTNAVKVED</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEN0</accession>
    <name>F6BEN0_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Translation initiation factor 6</fullName>
            <shortName evidence="3">aIF-6</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">eif6</name>
        <name evidence="4" type="ordered locus">Metig_1290</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Binds to the 50S ribosomal subunit and prevents its association with the 30S ribosomal subunit to form the 70S initiation complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the eIF-6 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96827.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799424.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1290"/>
    <dbReference type="EnsemblBacteria" id="AEF96827">
        <property type="protein sequence ID" value="AEF96827"/>
        <property type="gene ID" value="Metig_1290"/>
    </dbReference>
    <dbReference type="GeneID" id="10644153"/>
    <dbReference type="KEGG" id="mig:Metig_1290"/>
    <dbReference type="HOGENOM" id="CLU_071894_1_0_2"/>
    <dbReference type="OMA" id="GEDTTGP"/>
    <dbReference type="OrthoDB" id="70525at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042256">
        <property type="term" value="P:mature ribosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00032">
        <property type="entry name" value="eIF_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002769">
        <property type="entry name" value="eIF6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10784">
        <property type="entry name" value="PTHR10784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01912">
        <property type="entry name" value="eIF-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006413">
        <property type="entry name" value="IF-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00654">
        <property type="entry name" value="eIF6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00323">
        <property type="entry name" value="eIF-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3 4" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="2 3" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEF96827.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="226" mass="24231" checksum="CDCECBEB4D35E4A9" modified="2011-07-27" version="1">MIIRKYFSGISTLGVLALATEKFALLPYIVEENTVEEIKKVLDVPVTQLNVGGCSLIGSLCVANSYGLLLPNIVKDDEVELIKNFLEENDIDIQIKILNSKNTALGNLILTNDKGCIISEELKGFKKDIEDILNVDVDIGNIAELPTVGSNAVATNKGCLVHPLADDEELEWIKDVLKIDCIGRGTANKGVTSVGACIIANTKGAIVGGDTTGPELLRIEEALDLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEP0</accession>
    <name>F6BEP0_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Conserved repeat domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1300</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96837.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1300"/>
    <dbReference type="EnsemblBacteria" id="AEF96837">
        <property type="protein sequence ID" value="AEF96837"/>
        <property type="gene ID" value="Metig_1300"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_1300"/>
    <dbReference type="HOGENOM" id="CLU_240399_0_0_2"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039448">
        <property type="entry name" value="Beta_helix"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006626">
        <property type="entry name" value="PbH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012334">
        <property type="entry name" value="Pectin_lyas_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13229">
        <property type="entry name" value="Beta_helix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00710">
        <property type="entry name" value="PbH1"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="SSF51126"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Beta_helix" evidence="1">
        <location>
            <begin position="1314"/>
            <end position="1474"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96837.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="1716" mass="191484" checksum="4D3AE20FEE445D70" modified="2011-07-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-09-29" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEQ5</accession>
    <name>F6BEQ5_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1315</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96852.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799449.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96852">
        <property type="protein sequence ID" value="AEF96852"/>
        <property type="gene ID" value="Metig_1315"/>
    </dbReference>
    <dbReference type="GeneID" id="10644178"/>
    <dbReference type="KEGG" id="mig:Metig_1315"/>
    <dbReference type="HOGENOM" id="CLU_083802_0_0_2"/>
    <dbReference type="OMA" id="FELPEWE"/>
    <dbReference type="OrthoDB" id="74150at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025098">
        <property type="entry name" value="DUF4013"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13197">
        <property type="entry name" value="DUF4013"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="58"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="115"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="153"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="196"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="231"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96852.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="285" mass="32636" checksum="D164A3441729BC74" modified="2011-07-27" version="1">MFFESYIKEPLKYALSDTKKLFVGGVILLVGMISILFGMFLIILGIMPHMMPENIQHLQVFGVLGVILGGGFSLVGLLCSLVISGYYMQIIKYTLNDKNELPEWENFLDMIKKGFLYMVGVSILSILINIPSYLFAIIHVFNNSLIISILDNIISIICSLVAWLIIPLATVNFVAKDRFFAFFYLREIIRMMSFEYVGVLFAIAVISFVIIILYILVVVMFGVALWFVSKLLMVIIIALFILTFPFVDIFIKVFSNRAYAKYYKNYTNKIHKNNGETEKIHNETF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEV5</accession>
    <name>F6BEV5_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Queuosine synthesis-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0131</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95691.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798300.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0131"/>
    <dbReference type="EnsemblBacteria" id="AEF95691">
        <property type="protein sequence ID" value="AEF95691"/>
        <property type="gene ID" value="Metig_0131"/>
    </dbReference>
    <dbReference type="GeneID" id="10642966"/>
    <dbReference type="KEGG" id="mig:Metig_0131"/>
    <dbReference type="HOGENOM" id="CLU_077587_0_0_2"/>
    <dbReference type="OMA" id="YHPCGRC"/>
    <dbReference type="OrthoDB" id="66950at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012096">
        <property type="entry name" value="ATPase_PP-loop_MJ1638"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022310">
        <property type="entry name" value="NAD/GMP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02540">
        <property type="entry name" value="NAD_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006601">
        <property type="entry name" value="ATPase_UCP006601"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD_synthase" evidence="1">
        <location>
            <begin position="58"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF95691.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="254" mass="29201" checksum="C13ABAE521A0BC26" modified="2011-07-27" version="1">MEEIIKEIRLKASMKALERLKEENLIDLDAYNNLMNLLDRRIKGKKYFYKFDVEHKPTAVVAFSGGVDSSTSALISKNIFDVVGVTVYSNHIMHEEVKNHVKNLSKKLGIKHEFIDIDMDKIAKDIEKGKYHPCGRCHKAVEEAVINYAKNNGIKFVVFGDLLSVGYLSIIPMDDGLIRINMPAFLSLTKNENREILRQNNIEINLPYGCPLVKKAHEHKHMRKFTIQRILREVRAQVVDKDEGLKNILDMLNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEY6</accession>
    <name>F6BEY6_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">DNA topoisomerase type IA zn finger domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0164</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95722.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798331.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0164"/>
    <dbReference type="EnsemblBacteria" id="AEF95722">
        <property type="protein sequence ID" value="AEF95722"/>
        <property type="gene ID" value="Metig_0164"/>
    </dbReference>
    <dbReference type="GeneID" id="10642999"/>
    <dbReference type="KEGG" id="mig:Metig_0164"/>
    <dbReference type="HOGENOM" id="CLU_920144_0_0_2"/>
    <dbReference type="OMA" id="ANRDYNW"/>
    <dbReference type="OrthoDB" id="40374at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003916">
        <property type="term" value="F:DNA topoisomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006265">
        <property type="term" value="P:DNA topological change"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013498">
        <property type="entry name" value="Topo_IA_Znf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01396">
        <property type="entry name" value="zf-C4_Topoisom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0413">Isomerase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="zf-C4_Topoisom" evidence="1">
        <location>
            <begin position="253"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01396"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF95722.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="292" mass="34108" checksum="54A5E747CDAB3ECB" modified="2011-07-27" version="1">MLDELFSIMTKNIHFNATQLSEKTWNQNWKVPEGLISIIGIKNGKKPVFYYGVTNAYKKEYELKKGISPSGSRFINARVYYKFDRHDIIEKEKYVAANAFNGLLLCIKVDKDEFVNITKRMLLDGYKSGDEHIIEVLETTRDRNMFDTIEESALFIANRDYNWLIGKIKNATRLLRFSGQGYWLLPLKTKLEITSGFIIKGNELIVNLENVELFKNYLVYVDTKENVVRYNPNRLCNRGVFLVDEVRKMINENVCPWCGSKLRVVRTKKGEFLGCTSYPNCLYRRFPNKNTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F6BF04</accession>
    <name>F6BF04_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0173 metal-dependent hydrolase Metig_0183</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_0183</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0173 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95740.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798349.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0183"/>
    <dbReference type="EnsemblBacteria" id="AEF95740">
        <property type="protein sequence ID" value="AEF95740"/>
        <property type="gene ID" value="Metig_0183"/>
    </dbReference>
    <dbReference type="GeneID" id="10643018"/>
    <dbReference type="KEGG" id="mig:Metig_0183"/>
    <dbReference type="HOGENOM" id="CLU_070010_4_0_2"/>
    <dbReference type="OMA" id="WHGHSTW"/>
    <dbReference type="OrthoDB" id="76909at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00457">
        <property type="entry name" value="UPF0173"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022877">
        <property type="entry name" value="UPF0173"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="SSF56281"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Lactamase_B" evidence="2">
        <location>
            <begin position="6"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF95740.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="217" mass="23510" checksum="200F4D3C9846082C" modified="2011-07-27" version="1">MITWYGHACFKVDNVLIDPFVPNPLCDIPFDEIMEGVDVIAVTHGHGDHLGNAEKLSKEYNVPVVANHEISVYLQELGVCAEGMNIGGTIVINNAKLTMVKAEHSSDIAPNISGGVAGGFIVNDRVYHAGDTGVFSDMELIGEIYSPSIALLPIGGRYTMDIKEALIAIELLYPEVVIPMHYNTFPLIKADPNEFKKLAEGLGVEVIIPKIGEPIDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-06-02" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>F6BF06</accession>
    <name>F6BF06_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">VAR1 protein isolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_0185</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95742.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798351.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF95742">
        <property type="protein sequence ID" value="AEF95742"/>
        <property type="gene ID" value="Metig_0185"/>
    </dbReference>
    <dbReference type="GeneID" id="10643020"/>
    <dbReference type="KEGG" id="mig:Metig_0185"/>
    <dbReference type="HOGENOM" id="CLU_1264574_0_0_2"/>
    <dbReference type="OMA" id="VYVYLEF"/>
    <dbReference type="OrthoDB" id="81436at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF95742.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="218" mass="25182" checksum="2F0067538F5DE285" modified="2011-07-27" version="1">MRKFILFFVLINVLPVVIAGWYLYENIGGAESVDEVIENAPFGEFIYLDHNMIIANKDNMNNLHGIYKDLLIFINGIYISSDGKSFGIKMPLASTLKYVRIDNYTYYNGCVIKGNVQLEKPTSNDLITLIPQSFKDIVVYRKDSVIGGLIENNEIKYVWVFRKKKNINAKIIRTYLDNVKKHNPNLIDYSVIDYGDKVYVYLEYRGLSIELPNMNVIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>F6BF08</accession>
    <name>F6BF08_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Hydrogenase expression/formation protein HypD</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_0187</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the HypD family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95744.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0187"/>
    <dbReference type="EnsemblBacteria" id="AEF95744">
        <property type="protein sequence ID" value="AEF95744"/>
        <property type="gene ID" value="Metig_0187"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0187"/>
    <dbReference type="HOGENOM" id="CLU_048562_1_0_2"/>
    <dbReference type="OMA" id="HVCGSHE"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11750">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.20.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002780">
        <property type="entry name" value="Hyd_form_HypD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042243">
        <property type="entry name" value="HypD_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042244">
        <property type="entry name" value="HypD_2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30149">
        <property type="entry name" value="PTHR30149"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01924">
        <property type="entry name" value="HypD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005622">
        <property type="entry name" value="Hydrgn_mat_hypD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00075">
        <property type="entry name" value="hypD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF95744.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="390" mass="43918" checksum="E477DD6DA26317A3" modified="2011-07-27" version="1">MKLLEKVERGKPLSGFCSHKIKFGERMININDKQMIRKAIETINKLSQKVDDVKIMHVCGSHEHTICKYGIREVLPDNITVIPGPGCPVCVTTQKEIDTAIYLAENGYTIATLGDMYRVPGSEKSLMEAQSEGYDVRIVYGINDAVKIAKKEGNKVVFVAIGFETTAPTTAAELIGLKNKNDNINFYILNCHRQTPPVMEFLLNEGNVNLDGFICPGHVSTITGLKPYYEPCKKYNAPMVVAGFEPIDVLMAIIMILKQIINREAKVENEYKRVVREKGNVMAQKIMNEVFEPIDIPWRGFPVIKNGGLGLKEEYKKFDIFEVEDIPPIKEKIPKGCICDKILRGEKFPTDCKLFGTVCTPLNPVGSCMVSDEGTCRIFYKYKKFQMNEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>F6BF09</accession>
    <name>F6BF09_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Probable transaldolase</fullName>
            <ecNumber evidence="9">2.2.1.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">tal</name>
        <name evidence="10" type="ordered locus">Metig_0188</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Transaldolase is important for the balance of metabolites in the pentose-phosphate pathway.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>D-glyceraldehyde 3-phosphate + D-sedoheptulose 7-phosphate = beta-D-fructose 6-phosphate + D-erythrose 4-phosphate</text>
            <dbReference type="Rhea" id="RHEA:17053"/>
            <dbReference type="ChEBI" id="CHEBI:16897"/>
            <dbReference type="ChEBI" id="CHEBI:57483"/>
            <dbReference type="ChEBI" id="CHEBI:57634"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="2.2.1.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 9">Carbohydrate degradation; pentose phosphate pathway; D-glyceraldehyde 3-phosphate and beta-D-fructose 6-phosphate from D-ribose 5-phosphate and D-xylulose 5-phosphate (non-oxidative stage): step 2/3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 9">Belongs to the transaldolase family. Type 3B subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.2.1.2" evidence="9"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95745.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798354.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0188"/>
    <dbReference type="EnsemblBacteria" id="AEF95745">
        <property type="protein sequence ID" value="AEF95745"/>
        <property type="gene ID" value="Metig_0188"/>
    </dbReference>
    <dbReference type="GeneID" id="10643023"/>
    <dbReference type="KEGG" id="mig:Metig_0188"/>
    <dbReference type="HOGENOM" id="CLU_079764_0_0_2"/>
    <dbReference type="OMA" id="VRHPMHV"/>
    <dbReference type="OrthoDB" id="77186at2157"/>
    <dbReference type="UniPathway" id="UPA00115">
        <property type="reaction ID" value="UER00414"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016832">
        <property type="term" value="F:aldehyde-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004801">
        <property type="term" value="F:transaldolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006098">
        <property type="term" value="P:pentose-phosphate shunt"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00956">
        <property type="entry name" value="Transaldolase_FSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00494">
        <property type="entry name" value="Transaldolase_3b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001585">
        <property type="entry name" value="TAL/FSA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022999">
        <property type="entry name" value="Transaldolase_3B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004731">
        <property type="entry name" value="Transaldolase_3B/F6P_aldolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018225">
        <property type="entry name" value="Transaldolase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033919">
        <property type="entry name" value="TSA/FSA_arc/bac"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10683">
        <property type="entry name" value="PTHR10683"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00923">
        <property type="entry name" value="TAL_FSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00875">
        <property type="entry name" value="fsa_talC_mipB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01054">
        <property type="entry name" value="TRANSALDOLASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 9" id="KW-0570">Pentose shunt</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 9" id="KW-0704">Schiff base</keyword>
    <keyword evidence="6 9" id="KW-0808">Transferase</keyword>
    <feature type="active site" description="Schiff-base intermediate with substrate" evidence="9">
        <location>
            <position position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005740"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AEF95745.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="217" mass="24042" checksum="F3F22E58AE7B7377" modified="2011-07-27" version="1">MKFFLDTANVEQIKKFVELGLVDGVTTNPTLIAKEGKDFHTVIKEICEIVDGPVSAEVVSLDAEGMIKEARELASLADNVVIKIPMTKEGMKAVNVLSKEGIKTNVTLVFSPLQALIAAKAGATYVSPFVGRLDDIGHVGMKLIEDVVKIYRNYDIKTEVIVASVRHPMHVLEAARIGADIATIPFDVMDKMFKHPLTDIGIERFMKDWENYLKNKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>F6BF16</accession>
    <name>F6BF16_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="7">RNA methylase, NOL1/NOP2/sun family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Metig_0195</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the class I-like SAM-binding methyltransferase superfamily. RsmB/NOP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95752.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798361.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0195"/>
    <dbReference type="EnsemblBacteria" id="AEF95752">
        <property type="protein sequence ID" value="AEF95752"/>
        <property type="gene ID" value="Metig_0195"/>
    </dbReference>
    <dbReference type="GeneID" id="10643030"/>
    <dbReference type="KEGG" id="mig:Metig_0195"/>
    <dbReference type="HOGENOM" id="CLU_005316_7_0_2"/>
    <dbReference type="OMA" id="EPEENEF"/>
    <dbReference type="OrthoDB" id="32049at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001510">
        <property type="term" value="P:RNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018314">
        <property type="entry name" value="Fmu/NOL1/Nop2p_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031341">
        <property type="entry name" value="Methyltr_RsmF_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001678">
        <property type="entry name" value="MeTrfase_RsmB/NOP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011023">
        <property type="entry name" value="Nop2p"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023267">
        <property type="entry name" value="RCMT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22807">
        <property type="entry name" value="PTHR22807"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01189">
        <property type="entry name" value="Methyltr_RsmB-F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17125">
        <property type="entry name" value="Methyltr_RsmF_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02008">
        <property type="entry name" value="RCMTFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00446">
        <property type="entry name" value="nop2p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01153">
        <property type="entry name" value="NOL1_NOP2_SUN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51686">
        <property type="entry name" value="SAM_MT_RSMB_NOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5 7" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4 5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SAM_MT_RSMB_NOP" evidence="6">
        <location>
            <begin position="1"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="region of interest" description="S-adenosyl-L-methionine binding" evidence="5">
        <location>
            <begin position="84"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="5">
        <location>
            <position position="201"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="108"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="152"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEF95752.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="264" mass="29542" checksum="918400939C3782F1" modified="2011-07-27" version="1">MNLQYIRVNTLKINPETLKNRLENKGVMLKETFLPYAFEVLESPFSVGATPEYLFGYYFVQSISSMIPPIVLNPSKDDLVLDMCAAPGGKTTHLAQLMNNEGAIIAVEIKSSRMKSLKANINRMGIANVIMLNMNVLHLKEKDLKFDKILLDAPCSGNVIKDKSRSVTKEDIIYCSNRQKELIDAGIDLLKEGGVLVYSTCSAEEEENEGVIEYILNKRDDVELLPIENNFKGINVIEGGIEGTLRVIPPNEPFFIAKLMKIKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-06-02" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>F6BF34</accession>
    <name>F6BF34_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Transposase IS4 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_0311</name>
        <name evidence="4" type="ordered locus">Metig_0464</name>
        <name evidence="5" type="ordered locus">Metig_0532</name>
        <name evidence="6" type="ordered locus">Metig_0600</name>
        <name evidence="7" type="ordered locus">Metig_0647</name>
        <name evidence="8" type="ordered locus">Metig_0803</name>
        <name evidence="9" type="ordered locus">Metig_1048</name>
        <name evidence="10" type="ordered locus">Metig_1165</name>
        <name evidence="11" type="ordered locus">Metig_1177</name>
        <name evidence="12" type="ordered locus">Metig_1279</name>
        <name evidence="13" type="ordered locus">Metig_1369</name>
        <name evidence="14" type="ordered locus">Metig_1478</name>
        <name evidence="15" type="ordered locus">Metig_1577</name>
        <name evidence="16" type="ordered locus">Metig_1627</name>
        <name evidence="17" type="ordered locus">Metig_1685</name>
        <name evidence="18" type="ordered locus">Metig_1706</name>
    </gene>
    <organism evidence="19">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="13 19" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95867.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96020.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96087.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96154.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96197.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96348.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96589.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96703.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96715.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96816.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96904.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97012.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97111.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97160.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97217.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97238.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798476.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF95867">
        <property type="protein sequence ID" value="AEF95867"/>
        <property type="gene ID" value="Metig_0311"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96020">
        <property type="protein sequence ID" value="AEF96020"/>
        <property type="gene ID" value="Metig_0464"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96087">
        <property type="protein sequence ID" value="AEF96087"/>
        <property type="gene ID" value="Metig_0532"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96154">
        <property type="protein sequence ID" value="AEF96154"/>
        <property type="gene ID" value="Metig_0600"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96197">
        <property type="protein sequence ID" value="AEF96197"/>
        <property type="gene ID" value="Metig_0647"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96348">
        <property type="protein sequence ID" value="AEF96348"/>
        <property type="gene ID" value="Metig_0803"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96589">
        <property type="protein sequence ID" value="AEF96589"/>
        <property type="gene ID" value="Metig_1048"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96703">
        <property type="protein sequence ID" value="AEF96703"/>
        <property type="gene ID" value="Metig_1165"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96715">
        <property type="protein sequence ID" value="AEF96715"/>
        <property type="gene ID" value="Metig_1177"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96816">
        <property type="protein sequence ID" value="AEF96816"/>
        <property type="gene ID" value="Metig_1279"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96904">
        <property type="protein sequence ID" value="AEF96904"/>
        <property type="gene ID" value="Metig_1369"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF97012">
        <property type="protein sequence ID" value="AEF97012"/>
        <property type="gene ID" value="Metig_1478"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF97111">
        <property type="protein sequence ID" value="AEF97111"/>
        <property type="gene ID" value="Metig_1577"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF97160">
        <property type="protein sequence ID" value="AEF97160"/>
        <property type="gene ID" value="Metig_1627"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF97217">
        <property type="protein sequence ID" value="AEF97217"/>
        <property type="gene ID" value="Metig_1685"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF97238">
        <property type="protein sequence ID" value="AEF97238"/>
        <property type="gene ID" value="Metig_1706"/>
    </dbReference>
    <dbReference type="GeneID" id="10644579"/>
    <dbReference type="KEGG" id="mig:Metig_0311"/>
    <dbReference type="KEGG" id="mig:Metig_0464"/>
    <dbReference type="KEGG" id="mig:Metig_0532"/>
    <dbReference type="KEGG" id="mig:Metig_0600"/>
    <dbReference type="KEGG" id="mig:Metig_0647"/>
    <dbReference type="KEGG" id="mig:Metig_0803"/>
    <dbReference type="KEGG" id="mig:Metig_1048"/>
    <dbReference type="KEGG" id="mig:Metig_1165"/>
    <dbReference type="KEGG" id="mig:Metig_1177"/>
    <dbReference type="KEGG" id="mig:Metig_1279"/>
    <dbReference type="KEGG" id="mig:Metig_1369"/>
    <dbReference type="KEGG" id="mig:Metig_1478"/>
    <dbReference type="KEGG" id="mig:Metig_1577"/>
    <dbReference type="KEGG" id="mig:Metig_1627"/>
    <dbReference type="KEGG" id="mig:Metig_1685"/>
    <dbReference type="KEGG" id="mig:Metig_1706"/>
    <dbReference type="HOGENOM" id="CLU_893149_0_0_2"/>
    <dbReference type="OrthoDB" id="142307at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:transposition, DNA-mediated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002559">
        <property type="entry name" value="Transposase_11"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01609">
        <property type="entry name" value="DDE_Tnp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="domain" description="DDE_Tnp_1" evidence="2">
        <location>
            <begin position="161"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF95867.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEF96020.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEF96087.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEF96154.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEF96197.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEF96348.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AEF96589.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AEF96703.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AEF96715.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AEF96816.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AEF96904.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="AEF97012.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="AEF97111.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="AEF97160.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="AEF97217.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="AEF97238.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="311" mass="36361" checksum="CBEA1574650C32E7" modified="2011-07-27" version="1">MSGRKTKRKGYWKAYDKRFKIFNIKYTYDFVSFIINILLPYKEKRKVGRPLAISYNEYIATLIIKHIFRISLRDLETLSDYLHKKHIDSSTYGKAFQRIKISDLTKIIVGLHLIIANSLKSSVIIYIADSTGVHLLRVYCERIRVVNNEIKKVKYRVFDKMHVLACYYKDYGLISIVMVKWDNGYSSDSKNLLKMIKSLDFVKGAIILLDGGYDDEDLLRELLSIDLIPIVKTKEFKWDYGISKIRKKVKKLFDKKLYKIRGVIEAIFGGLKTKFRLTLNEKLPESRCRATLAVAIVHNILTLMRVISIRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAG1</accession>
    <name>F6BAG1_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="10">DEAD/DEAH box helicase domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Metig_1439</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the DEAD box helicase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96974.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799570.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1439"/>
    <dbReference type="EnsemblBacteria" id="AEF96974">
        <property type="protein sequence ID" value="AEF96974"/>
        <property type="gene ID" value="Metig_1439"/>
    </dbReference>
    <dbReference type="GeneID" id="10644312"/>
    <dbReference type="KEGG" id="mig:Metig_1439"/>
    <dbReference type="HOGENOM" id="CLU_003041_21_1_2"/>
    <dbReference type="OMA" id="EHKDGQR"/>
    <dbReference type="OrthoDB" id="34464at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 6 10" id="KW-0347">Helicase</keyword>
    <keyword evidence="2 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Q_MOTIF" evidence="9">
        <location>
            <begin position="3"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="7">
        <location>
            <begin position="35"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="8">
        <location>
            <begin position="216"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="5">
        <location>
            <begin position="3"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AEF96974.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="434" mass="49242" checksum="B07139F21CC2A2B2" modified="2011-07-27" version="1">MNLSFKELGLSENTLKALEKKGFKEPTEIQEKTIPILLNGDVDVVGQAQTGTGKTAAFGLPLIEKIDENSKDVQALILTPTRELAIQVSEEIDSLKGEKKLKILPVYGGQPIEPQIRQLKRGVHIVVGTPGRILDHIRRGSLNLKNISYLVLDEADEMLNMGFIDDVEEILRHTNKNKRVLLFSATLPRTILNLAKRYMEEYRLVSVKKETLTTNLVEQIYYEVSNSKKFEALCRVIDVEDDFYGLVFCKTRADVNEVANKLIEKGYEADALHGDIAQKQRERILNRFKKKRINILVATDVAARGIDINNLTHVINYSLPQNPESYVHRIGRTGRAGKKGTAISFVTPNEYRKLKYIKKIAKVNIRKEKLPEIDDVINAKKSKILNNIVEIIKSEDYQEYVEISQELINEFGAERALAALLKYVFKDKLVIIGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAK2</accession>
    <name>F6BAK2_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="3">AAA ATPase central domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_1481</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AAA ATPase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97015.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799610.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1481"/>
    <dbReference type="EnsemblBacteria" id="AEF97015">
        <property type="protein sequence ID" value="AEF97015"/>
        <property type="gene ID" value="Metig_1481"/>
    </dbReference>
    <dbReference type="GeneID" id="10644354"/>
    <dbReference type="KEGG" id="mig:Metig_1481"/>
    <dbReference type="HOGENOM" id="CLU_000688_21_3_2"/>
    <dbReference type="OMA" id="FGEWAPK"/>
    <dbReference type="OrthoDB" id="41252at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA" evidence="2">
        <location>
            <begin position="153"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU003651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF97015.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="370" mass="41907" checksum="D3BF33169F8C7146" modified="2011-07-27" version="1">MSNINVNTIRIKPLFSKSLPLPDETMALKYVVLEPVGFPIKVNSEKVKVTTDDPKLFNVYARDQWAGEIVKEGDYLFDNTIIPDYAFKVISTYPREGGIVTKDTLFKLETPKVVKRDFKKARFSDVIGQEEAKRKCKIIMKYLENPKLFGEWAPKNVLFYGPPGTGKTLLARALATETDVPLFLIKAPELIGEHVGDGSKQIRELYENASENAPCIVFIDELDAIALSRQYQSLRGDVSEVVNALLTELDGIKDNEGVVTIAATNNPNMLDPAIRSRFEEEIEFKLPNDKERLKIMELYAKKMPIPIKADLRKYVEKTKGMSGRDIKEKFLKPALHKAILEDRDYISKEDLDAALKKILGDKKEPLHLYG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAL7</accession>
    <name>F6BAL7_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Poly(ADP-ribose) glycohydrolase</fullName>
            <ecNumber evidence="1">3.2.1.143</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_1496</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.2.1.143" evidence="1"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97030.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799624.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1496"/>
    <dbReference type="EnsemblBacteria" id="AEF97030">
        <property type="protein sequence ID" value="AEF97030"/>
        <property type="gene ID" value="Metig_1496"/>
    </dbReference>
    <dbReference type="GeneID" id="10644369"/>
    <dbReference type="KEGG" id="mig:Metig_1496"/>
    <dbReference type="HOGENOM" id="CLU_024566_3_0_2"/>
    <dbReference type="OMA" id="IPPSWEQ"/>
    <dbReference type="OrthoDB" id="74062at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004649">
        <property type="term" value="F:poly(ADP-ribose) glycohydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008152">
        <property type="term" value="P:metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4080.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005502">
        <property type="entry name" value="Ribosyl_crysJ1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036705">
        <property type="entry name" value="Ribosyl_crysJ1_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03747">
        <property type="entry name" value="ADP_ribosyl_GH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101478">
        <property type="entry name" value="SSF101478"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF97030.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="298" mass="33007" checksum="D7A8CA490FEC9ACE" modified="2011-07-27" version="1">MLNKFKGCLVGVAIGDAMGMPSEGLTKEQIKSIYGIIDDYKNPENHFKPYLKAGQYTDDTQQTIALAEAITKEGFSDELFIKNLIEWYKNEPRGIGPTSEKAIKNLINGKLEGINSKTCGAAMRVAPLALYCRDLDELKRNIIEASKITHNNDEAIAGALAVGFFIFNALKDRKDEELVNECSEFINNVCPKFSERIGLIKDIRDIEKGFEIFGTGIDAIECVPSAILCFLRTDNFKDAIIKAVNAGGDTDSLGAMVGAMAGAYYGYNNIPKKWIEGLENRDYIISLAEKLYKLRFEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAP0</accession>
    <name>F6BAP0_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Leucine--tRNA ligase</fullName>
            <ecNumber evidence="7">6.1.1.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Leucyl-tRNA synthetase</fullName>
            <shortName evidence="7">LeuRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">leuS</name>
        <name evidence="11" type="ordered locus">Metig_0298</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu)</text>
            <dbReference type="Rhea" id="RHEA:11688"/>
            <dbReference type="Rhea" id="RHEA-COMP:9613"/>
            <dbReference type="Rhea" id="RHEA-COMP:9622"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57427"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78494"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.4"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7 8">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.4" evidence="7"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95854.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798463.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0298"/>
    <dbReference type="EnsemblBacteria" id="AEF95854">
        <property type="protein sequence ID" value="AEF95854"/>
        <property type="gene ID" value="Metig_0298"/>
    </dbReference>
    <dbReference type="GeneID" id="10643133"/>
    <dbReference type="KEGG" id="mig:Metig_0298"/>
    <dbReference type="HOGENOM" id="CLU_004174_0_0_2"/>
    <dbReference type="OMA" id="AWNMAFQ"/>
    <dbReference type="OrthoDB" id="4914at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002161">
        <property type="term" value="F:aminoacyl-tRNA editing activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004823">
        <property type="term" value="F:leucine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006429">
        <property type="term" value="P:leucyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.740.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00049_A">
        <property type="entry name" value="Leu_tRNA_synth_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002300">
        <property type="entry name" value="aa-tRNA-synth_Ia"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020791">
        <property type="entry name" value="Leu-tRNA-lgase_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004493">
        <property type="entry name" value="Leu-tRNA-synth_Ia_arc/euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013155">
        <property type="entry name" value="M/V/L/I-tRNA-synth_anticd-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009008">
        <property type="entry name" value="Val/Leu/Ile-tRNA-synth_edit"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45794">
        <property type="entry name" value="PTHR45794"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08264">
        <property type="entry name" value="Anticodon_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00133">
        <property type="entry name" value="tRNA-synt_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="SSF47323"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50677">
        <property type="entry name" value="SSF50677"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00395">
        <property type="entry name" value="leuS_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7 8" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="4 7 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 7 8" id="KW-0436">Ligase</keyword>
    <keyword evidence="3 7 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 7 8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="tRNA-synt_1" evidence="9">
        <location>
            <begin position="11"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="domain" description="Anticodon_1" evidence="10">
        <location>
            <begin position="711"/>
            <end position="840"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'HIGH' region" evidence="7">
        <location>
            <begin position="39"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'KMSKS' region" evidence="7">
        <location>
            <begin position="634"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="637"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF08264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AEF95854.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="944" mass="110387" checksum="C6252D562263C7A2" modified="2011-07-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAP3</accession>
    <name>F6BAP3_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">FeMo cofactor biosynthesis protein NifB</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Nitrogenase cofactor maturase NifB</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Radical SAM assemblase NifB</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">Metig_0301</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Cofactor biosynthesis; Fe-Mo cofactor biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the radical SAM superfamily. NifB family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95857.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798466.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0301"/>
    <dbReference type="EnsemblBacteria" id="AEF95857">
        <property type="protein sequence ID" value="AEF95857"/>
        <property type="gene ID" value="Metig_0301"/>
    </dbReference>
    <dbReference type="GeneID" id="10643136"/>
    <dbReference type="KEGG" id="mig:Metig_0301"/>
    <dbReference type="HOGENOM" id="CLU_027639_1_0_2"/>
    <dbReference type="OMA" id="PCYNEKL"/>
    <dbReference type="OrthoDB" id="46264at2157"/>
    <dbReference type="UniPathway" id="UPA00782"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009399">
        <property type="term" value="P:nitrogen fixation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaB/NifB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005980">
        <property type="entry name" value="Nase_CF_NifB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43787:SF3">
        <property type="entry name" value="PTHR43787:SF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0408">Iron</keyword>
    <keyword evidence="8" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0535">Nitrogen fixation</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="12">
        <location>
            <begin position="22"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00030926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00032102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AEF95857.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="294" mass="33676" checksum="28E144DEBC9463C1" modified="2011-07-27" version="1">MDKMSKFSHITKVHPCYNEKLHDKIGRVHLPVAPKCNIACRFCKRSISEDCCEHRPGVSHGVLKPEDVEDYLKEIIKKVPNLKVVGIAGPGDSLFNKETFETFEIINDKFPDLIKCLSTNGLLLPKYAKKLADLDVKTVTVTVNAIDPKILKDIVDWVYYDKKVYRGEEGAKILIENQIKGIKKAYDEGLIIKINTVLIPEINLEHVVEIAKTLKDYAYIQNIIPLIPMYKMKNLRRPTCMEMNTVKDKCEEYIKQFRACQQCRADAVGLLSEAKILKEKGLNLDIYDLKHFSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAR0</accession>
    <name>F6BAR0_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_0318</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95874.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798483.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0318"/>
    <dbReference type="EnsemblBacteria" id="AEF95874">
        <property type="protein sequence ID" value="AEF95874"/>
        <property type="gene ID" value="Metig_0318"/>
    </dbReference>
    <dbReference type="GeneID" id="10643154"/>
    <dbReference type="KEGG" id="mig:Metig_0318"/>
    <dbReference type="HOGENOM" id="CLU_086012_0_0_2"/>
    <dbReference type="OMA" id="PVPFWDK"/>
    <dbReference type="OrthoDB" id="49651at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002808">
        <property type="entry name" value="AdoCbi_amidolase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01955">
        <property type="entry name" value="CbiZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF95874.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="266" mass="29998" checksum="187E8C65A8412A6B" modified="2011-07-27" version="1">MRRKETIKVKIVKEKVLDMDDWRAYKISHTVEIENDAEKTKTLIIEFENKRRVLSTREGFKEVKYVGNHSVPVPFWDKVHNYKDYESHVLNEIGIKKEDIALLSTGANMDNLAVAKEEFDEFYVIAFTTAGAKYNAIRLGDEEADYIEKDFKTYKIVDGKLIPKEKVGTVNIILITNANLTDGAMVRAIITITEAKTNAFQELNIRSTKHPELLATGTGTDNVVVVKGFGRGVDYTGGHTKMGEMIAKAVKRSVIEALIKQDGIKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-06-02" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAR6</accession>
    <name>F6BAR6_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_0324</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95880.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798489.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF95880">
        <property type="protein sequence ID" value="AEF95880"/>
        <property type="gene ID" value="Metig_0324"/>
    </dbReference>
    <dbReference type="GeneID" id="10643160"/>
    <dbReference type="KEGG" id="mig:Metig_0324"/>
    <dbReference type="HOGENOM" id="CLU_2712841_0_0_2"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF95880.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="72" mass="8395" checksum="44FE17E5F61DDACF" modified="2011-07-27" version="1">MKNIDDEAIQILINAPLMSNDELKEALSYFKKLAMKKGIKKMFLAGSNLPFTEKFIYYIYTITKPDPDRYRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAS9</accession>
    <name>F6BAS9_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_0337</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95893.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798502.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF95893">
        <property type="protein sequence ID" value="AEF95893"/>
        <property type="gene ID" value="Metig_0337"/>
    </dbReference>
    <dbReference type="GeneID" id="10643174"/>
    <dbReference type="KEGG" id="mig:Metig_0337"/>
    <dbReference type="HOGENOM" id="CLU_1821029_0_0_2"/>
    <dbReference type="OMA" id="VGFHKDD"/>
    <dbReference type="OrthoDB" id="105601at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF95893.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="138" mass="15095" checksum="460818C3F7D4D706" modified="2011-07-27" version="1">MFTMGIKEKTLSVKCVEGNIVLELKKEDGEEISSITLDKESASLFVVLLNEGIVVAEKINKDFKKEGIMLKKIQDVGTCFASDGRLSIAVLPADEKRTASMIIGMHKENGHTSIVMKPKKAAYLGLAITEIIKSLINN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAT6</accession>
    <name>F6BAT6_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Methanogenesis marker protein 17</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_0344</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95900.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798509.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0344"/>
    <dbReference type="EnsemblBacteria" id="AEF95900">
        <property type="protein sequence ID" value="AEF95900"/>
        <property type="gene ID" value="Metig_0344"/>
    </dbReference>
    <dbReference type="GeneID" id="10643181"/>
    <dbReference type="KEGG" id="mig:Metig_0344"/>
    <dbReference type="HOGENOM" id="CLU_121275_0_0_2"/>
    <dbReference type="OMA" id="FIFVASE"/>
    <dbReference type="OrthoDB" id="93447at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016762">
        <property type="entry name" value="UCP019464_methan"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09886">
        <property type="entry name" value="DUF2113"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF019464">
        <property type="entry name" value="UCP019464"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03291">
        <property type="entry name" value="methan_mark_17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF95900.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="187" mass="21714" checksum="4A8537B270A0ECA5" modified="2011-07-27" version="1">MAEIFVDCKDEAGKKIYTKIIQTSLEDLILGKSILEVRMIIREDEPYFIIGVLPKQTTKLIRLRDFANIEETKKVDGKTIYKIKIEDETYLPQLLEKINIIDQPSRFEVVTDSPVDLDMVVYDAKKDFIAKVLDFMNRVFPEGMRVRKTFYGKAIASIASEKPFKKEWLDVALKLKEELENTKIIGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAV7</accession>
    <name>F6BAV7_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1510</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97044.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799638.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF97044">
        <property type="protein sequence ID" value="AEF97044"/>
        <property type="gene ID" value="Metig_1510"/>
    </dbReference>
    <dbReference type="GeneID" id="10644383"/>
    <dbReference type="KEGG" id="mig:Metig_1510"/>
    <dbReference type="HOGENOM" id="CLU_942037_0_0_2"/>
    <dbReference type="OMA" id="TLRSYHE"/>
    <dbReference type="OrthoDB" id="112190at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="30"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF97044.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="246" mass="28580" checksum="3F49E127A861E799" modified="2011-07-27" version="1">MNILRVIGGLCVIFGISLGIYGYLEDIFYFFNIGVGLILIGSIMTIFSYEKYEYDINTKLFNDYIDLIKRLIRSLEIENKGIVISKGGNIRSGVIFLPIEKDYRINLSLIDENILLVNSDNKKEMGLVFPLLGKSMLKILEREEFSNINTENLEDCLSYTLSLFNFGGDVVVEFKGREVIINYRIKNAEFCERMQKEKLCERFPCHVCGFIILVISRVLNRMLKIEEIKKDKDRIRIRLMVLENEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAW8</accession>
    <name>F6BAW8_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glycosyl transferase family 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1521</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97055.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799649.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1521"/>
    <dbReference type="EnsemblBacteria" id="AEF97055">
        <property type="protein sequence ID" value="AEF97055"/>
        <property type="gene ID" value="Metig_1521"/>
    </dbReference>
    <dbReference type="GeneID" id="10644394"/>
    <dbReference type="KEGG" id="mig:Metig_1521"/>
    <dbReference type="HOGENOM" id="CLU_033536_7_4_2"/>
    <dbReference type="OMA" id="HRILFFW"/>
    <dbReference type="OrthoDB" id="17570at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glyco_trans_2-like" evidence="1">
        <location>
            <begin position="11"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF97055.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="232" mass="26375" checksum="A9222C882DE8B7BA" modified="2011-07-27" version="1">MDKKGKKLLALIPAYNEEKSIKTVIENIKDIADGILVVDDGSTDNTSKYAKECGAEVIRFEKNRGKGAAIREGFRYFINSPYDICFIFDADGQYRKEDILPVCKPIINDEADIVVGSRFLGEYKEGANINYRIRILCNQISTYVTRLMSGLPTTDAQSGLVAVSKYSAKQLDLKADRWGIHQEIIIRAGKKGLRYKEVPIVFEKRMHGVSRLKVLKYPFTAFPVMLRAWIRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-06-02" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAX3</accession>
    <name>F6BAX3_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1526</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97060.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799654.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF97060">
        <property type="protein sequence ID" value="AEF97060"/>
        <property type="gene ID" value="Metig_1526"/>
    </dbReference>
    <dbReference type="GeneID" id="10644399"/>
    <dbReference type="KEGG" id="mig:Metig_1526"/>
    <dbReference type="HOGENOM" id="CLU_465907_0_0_2"/>
    <dbReference type="OMA" id="INCVISK"/>
    <dbReference type="OrthoDB" id="132920at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="5"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="98"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="136"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="161"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="191"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="214"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="245"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="323"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="356"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="382"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="414"/>
            <end position="431"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF97060.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="585" mass="67776" checksum="57599E8071750788" modified="2011-07-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAY7</accession>
    <name>F6BAY7_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DNA-directed RNA polymerase</fullName>
            <ecNumber evidence="2">2.7.7.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">Metig_1540</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">DNA-dependent RNA polymerase (RNAP) catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. The Rpo2 subunit (Rpo2N and Rpo2C in this organism) is implicated in DNA promoter recognition and in nucleotide binding.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Part of the RNA polymerase complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the RNA polymerase beta chain family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="2"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97074.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799668.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1540"/>
    <dbReference type="EnsemblBacteria" id="AEF97074">
        <property type="protein sequence ID" value="AEF97074"/>
        <property type="gene ID" value="Metig_1540"/>
    </dbReference>
    <dbReference type="GeneID" id="10644413"/>
    <dbReference type="KEGG" id="mig:Metig_1540"/>
    <dbReference type="HOGENOM" id="CLU_000524_5_3_2"/>
    <dbReference type="OMA" id="GKICPSE"/>
    <dbReference type="OrthoDB" id="14180at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed 5'-3' RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032549">
        <property type="term" value="F:ribonucleoside binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015712">
        <property type="entry name" value="DNA-dir_RNA_pol_su2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007644">
        <property type="entry name" value="RNA_pol_bsu_protrusion"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007642">
        <property type="entry name" value="RNA_pol_Rpb2_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037034">
        <property type="entry name" value="RNA_pol_Rpb2_2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007645">
        <property type="entry name" value="RNA_pol_Rpb2_3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20856">
        <property type="entry name" value="PTHR20856"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04563">
        <property type="entry name" value="RNA_pol_Rpb2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04561">
        <property type="entry name" value="RNA_pol_Rpb2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04565">
        <property type="entry name" value="RNA_pol_Rpb2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 14" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="5 14" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 14" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="RNA_pol_Rpb2_1" evidence="12">
        <location>
            <begin position="16"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="domain" description="RNA_pol_Rpb2_2" evidence="11">
        <location>
            <begin position="168"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="domain" description="RNA_pol_Rpb2_3" evidence="13">
        <location>
            <begin position="413"/>
            <end position="477"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00025096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00025838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF04561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF04563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF04565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="AEF97074.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="503" mass="57309" checksum="0DFD79EA060BF2F4" modified="2011-07-27" version="1">MESRVLVDAFFKEHSLVKHHIDSYNDFIENKLQKIVDEVGILETEIKGGYKIKFGKIRVGKPITKEADGSVRKITPMEARIRDLSYSAPLYLEMTPIIGEGEDEREGETVEVYIGELPVMLGSKICHLYGKSREELIELGEDPEDPFGYFIINGSEKVLVTQEDLIPNRILCEKVERNNKIIDVAKVFSTRHGFRALCTVERHPDGLLYATFPGMPGNVPLMILMKALGAESDRDIIEMISDDPDIVMQLVANLQDMREEHNVYTREDALEYIGKRVAPGQPKDYRLKRAETVLCNYLLPHVGVEKEDLIKKCWYLGKMAKTAIELYLGLRVEDDKDHYANKRLKLAGELMEDLFRYAFSQLVKDIKYQLERQTLRNKEPSIQAAVRSDVLTERIRHAMATGNWVGGRTGVSQLLDRTSYLATISQLRRVVSPLSRSQPHFEARDLHATQWGKICPSETPEGPNCGLVKNFALMCKVTTDEDDSKVIELLKEFGLNQNETFDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>F6BB26</accession>
    <name>F6BB26_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="3">dUTP diphosphatase</fullName>
            <ecNumber evidence="3">3.6.1.23</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_0355</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.6.1.23" evidence="3"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95911.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798520.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0355"/>
    <dbReference type="EnsemblBacteria" id="AEF95911">
        <property type="protein sequence ID" value="AEF95911"/>
        <property type="gene ID" value="Metig_0355"/>
    </dbReference>
    <dbReference type="GeneID" id="10643192"/>
    <dbReference type="KEGG" id="mig:Metig_0355"/>
    <dbReference type="HOGENOM" id="CLU_103451_2_0_2"/>
    <dbReference type="OMA" id="WDAGYEG"/>
    <dbReference type="OrthoDB" id="94658at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004170">
        <property type="term" value="F:dUTP diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd07557">
        <property type="entry name" value="trimeric_dUTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029054">
        <property type="entry name" value="dUTPase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036157">
        <property type="entry name" value="dUTPase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033704">
        <property type="entry name" value="dUTPase_trimeric"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00692">
        <property type="entry name" value="dUTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51283">
        <property type="entry name" value="SSF51283"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="dUTPase" evidence="2">
        <location>
            <begin position="60"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF95911.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="156" mass="17597" checksum="0BB2622D7134F6E2" modified="2011-07-27" version="1">MLIGAKISKNFVKVKDNIQIQQCGIDLRVGKIYKIVGKGTIDFTNEKRKLPAYIEVFDSEKDVKIDLEPGVYIVKTADKIKVPENVAGFAYPRSSLLRMGATLYTAVHDPGYEGYPAYLLHVLNPITIYKHARIAQIVFVECRDVAGLYEGIYNER</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-06-02" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>F6BB30</accession>
    <name>F6BB30_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="4">NADH ubiquinone oxidoreductase 20 kDa subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metig_0359</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FrhG family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95915.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798524.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF95915">
        <property type="protein sequence ID" value="AEF95915"/>
        <property type="gene ID" value="Metig_0359"/>
    </dbReference>
    <dbReference type="GeneID" id="10643196"/>
    <dbReference type="KEGG" id="mig:Metig_0359"/>
    <dbReference type="HOGENOM" id="CLU_075477_0_0_2"/>
    <dbReference type="OMA" id="INDEFCT"/>
    <dbReference type="OrthoDB" id="71699at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.700">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006137">
        <property type="entry name" value="NADH_UbQ_OxRdtase-like_20kDa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037024">
        <property type="entry name" value="NiFe_Hase_small_N_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13187">
        <property type="entry name" value="Fer4_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01058">
        <property type="entry name" value="Oxidored_q6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0830">Ubiquinone</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="3">
        <location>
            <begin position="150"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="3">
        <location>
            <begin position="180"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEF95915.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="203" mass="22215" checksum="60D739DC7284F414" modified="2011-07-27" version="1">MVECGSCGRIVDKIMKKYNLKDIALISGAVNLDDGEEVEKLKKIRENSKIVIAVGSCAVSGGFHRMLIGLENGFPQRFVRIGDVIKVDYAIIGCYPEEKEVEKVVEAILNNDEETLKPYRVIAGEPIIKPYMTVDEVLLTSNKELCLGCDTSPIENTYCTGCGTCVAKCPADALNIDEKPTVNMDKCVKCGTCFFSCIKVGVM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>F6BB41</accession>
    <name>F6BB41_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Ribonuclease HII</fullName>
            <shortName evidence="10">RNase HII</shortName>
            <ecNumber evidence="10">3.1.26.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">rnhB</name>
        <name evidence="13" type="ordered locus">Metig_0370</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3 10 11">Endonuclease that specifically degrades the RNA of RNA-DNA hybrids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 11">
            <text>Endonucleolytic cleavage to 5'-phosphomonoester.</text>
            <dbReference type="EC" id="3.1.26.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <cofactor evidence="10">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="10">Manganese or magnesium. Binds 1 divalent metal ion per monomer in the absence of substrate. May bind a second metal ion after substrate binding.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4 10">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 10 11">Belongs to the RNase HII family.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.4" evidence="10"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95926.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798535.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0370"/>
    <dbReference type="EnsemblBacteria" id="AEF95926">
        <property type="protein sequence ID" value="AEF95926"/>
        <property type="gene ID" value="Metig_0370"/>
    </dbReference>
    <dbReference type="GeneID" id="10643207"/>
    <dbReference type="KEGG" id="mig:Metig_0370"/>
    <dbReference type="HOGENOM" id="CLU_036532_0_4_2"/>
    <dbReference type="OMA" id="REECRFF"/>
    <dbReference type="OrthoDB" id="105421at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004523">
        <property type="term" value="F:RNA-DNA hybrid ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006401">
        <property type="term" value="P:RNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.460">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00052_A">
        <property type="entry name" value="RNase_HII_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004649">
        <property type="entry name" value="RNase_H2_suA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001352">
        <property type="entry name" value="RNase_HII/HIII"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024567">
        <property type="entry name" value="RNase_HII/HIII_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020787">
        <property type="entry name" value="RNase_HII_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023160">
        <property type="entry name" value="RNase_HII_hlx-loop-hlx_cap_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10954">
        <property type="entry name" value="PTHR10954"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01351">
        <property type="entry name" value="RNase_HII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00729">
        <property type="entry name" value="TIGR00729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 10 11" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="9 10 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0464">Manganese</keyword>
    <keyword evidence="7 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 10 11" id="KW-0540">Nuclease</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RNase_HII" evidence="12">
        <location>
            <begin position="8"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="11"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="12"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="113"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00007383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01351"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AEF95926.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="232" mass="26193" checksum="6BA8AB1BF08C9635" modified="2011-07-27" version="1">MEDKKIIIGIDEAGRGPVLGPMVVAGVAVEEDKIKELEDLGVKDSKQLTKNQRKKLFQLIGEMGKVKTIIIEPEKLDELMKTKSLNKIELNAFSKIANGFLKEIEGNFEIYVDACSSNAKAFANQLRAKMIDKSPKIIAEHKADEKYPIVSAASIIAKVTRDEIIESYKKEYGEIGSGYPSDPKTINFLKKYVEEFGELPKIARKSWKTAKRILEENKKENKKVKTLLDYIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>F6BB52</accession>
    <name>F6BB52_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="14 15">Dihydroxy-acid dehydratase</fullName>
            <shortName evidence="15">DAD</shortName>
            <ecNumber evidence="14 15">4.2.1.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">ilvD</name>
        <name evidence="16" type="ordered locus">Metig_0381</name>
    </gene>
    <organism evidence="17">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Functions in the biosynthesis of branched-chain amino acids. Catalyzes the dehydration of (2R,3R)-2,3-dihydroxy-3-methylpentanoate (2,3-dihydroxy-3-methylvalerate) into 2-oxo-3-methylpentanoate (2-oxo-3-methylvalerate) and of (2R)-2,3-dihydroxy-3-methylbutanoate (2,3-dihydroxyisovalerate) into 2-oxo-3-methylbutanoate (2-oxoisovalerate), the penultimate precursor to L-isoleucine and L-valine, respectively.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>(2R)-2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H2O</text>
            <dbReference type="Rhea" id="RHEA:24809"/>
            <dbReference type="ChEBI" id="CHEBI:11851"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:49072"/>
            <dbReference type="EC" id="4.2.1.9"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:24810"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>(2R,3R)-2,3-dihydroxy-3-methylpentanoate = (S)-3-methyl-2-oxopentanoate + H2O</text>
            <dbReference type="Rhea" id="RHEA:27694"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:35146"/>
            <dbReference type="ChEBI" id="CHEBI:49258"/>
            <dbReference type="EC" id="4.2.1.9"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15">
            <name>2Fe-2S cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49601"/>
        </cofactor>
        <text evidence="15">Binds 1 [2Fe-2S] cluster per subunit. This cluster acts as a Lewis acid cofactor.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 15">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="13 15">Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 3/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="12 15">Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 3/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="15">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the IlvD/Edd family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.9" evidence="14 15"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95937.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798546.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0381"/>
    <dbReference type="EnsemblBacteria" id="AEF95937">
        <property type="protein sequence ID" value="AEF95937"/>
        <property type="gene ID" value="Metig_0381"/>
    </dbReference>
    <dbReference type="GeneID" id="10643218"/>
    <dbReference type="KEGG" id="mig:Metig_0381"/>
    <dbReference type="HOGENOM" id="CLU_014271_4_2_2"/>
    <dbReference type="OMA" id="TQGRNMA"/>
    <dbReference type="OrthoDB" id="7176at2157"/>
    <dbReference type="UniPathway" id="UPA00047">
        <property type="reaction ID" value="UER00057"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00049">
        <property type="reaction ID" value="UER00061"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004160">
        <property type="term" value="F:dihydroxy-acid dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009097">
        <property type="term" value="P:isoleucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009099">
        <property type="term" value="P:valine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00012">
        <property type="entry name" value="IlvD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042096">
        <property type="entry name" value="Dihydro-acid_dehy_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004404">
        <property type="entry name" value="DihydroxyA_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000581">
        <property type="entry name" value="DiOHA_6PGluconate_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020558">
        <property type="entry name" value="DiOHA_6PGluconate_deHydtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037237">
        <property type="entry name" value="IlvD/EDD_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00920">
        <property type="entry name" value="ILVD_EDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143975">
        <property type="entry name" value="SSF143975"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00110">
        <property type="entry name" value="ilvD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00886">
        <property type="entry name" value="ILVD_EDD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00887">
        <property type="entry name" value="ILVD_EDD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 15" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="3 15" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="10 15" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="7 15" id="KW-0408">Iron</keyword>
    <keyword evidence="8 15" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="9 15 16" id="KW-0456">Lyase</keyword>
    <keyword evidence="6 15" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Proton acceptor" evidence="15">
        <location>
            <position position="467"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="78"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="120"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" description="via carbamate group" evidence="15">
        <location>
            <position position="121"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="441"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="N6-carboxylysine" evidence="15">
        <location>
            <position position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00029304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00029436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00029437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00029490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="AEF95937.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="552" mass="59295" checksum="BBF5A3E4D753EC68" modified="2011-07-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>F6BB53</accession>
    <name>F6BB53_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 3">Diaminopimelate decarboxylase</fullName>
            <shortName evidence="2">DAP decarboxylase</shortName>
            <shortName evidence="2">DAPDC</shortName>
            <ecNumber evidence="2 3">4.1.1.20</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">lysA</name>
        <name evidence="6" type="ordered locus">Metig_0382</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Specifically catalyzes the decarboxylation of meso-diaminopimelate (meso-DAP) to L-lysine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 3">
            <text>H(+) + meso-2,6-diaminoheptanedioate = CO2 + L-lysine</text>
            <dbReference type="Rhea" id="RHEA:15101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:32551"/>
            <dbReference type="ChEBI" id="CHEBI:57791"/>
            <dbReference type="EC" id="4.1.1.20"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 2 3">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2 3">Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; L-lysine from DL-2,6-diaminopimelate: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Orn/Lys/Arg decarboxylase class-II family. LysA subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.20" evidence="2 3"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95938.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798547.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0382"/>
    <dbReference type="EnsemblBacteria" id="AEF95938">
        <property type="protein sequence ID" value="AEF95938"/>
        <property type="gene ID" value="Metig_0382"/>
    </dbReference>
    <dbReference type="GeneID" id="10643219"/>
    <dbReference type="KEGG" id="mig:Metig_0382"/>
    <dbReference type="HOGENOM" id="CLU_026444_0_1_2"/>
    <dbReference type="OMA" id="YCRSMAS"/>
    <dbReference type="OrthoDB" id="20185at2157"/>
    <dbReference type="UniPathway" id="UPA00034">
        <property type="reaction ID" value="UER00027"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008836">
        <property type="term" value="F:diaminopimelate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009089">
        <property type="term" value="P:lysine biosynthetic process via diaminopimelate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06828">
        <property type="entry name" value="PLPDE_III_DapDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.37.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02120">
        <property type="entry name" value="LysA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009006">
        <property type="entry name" value="Ala_racemase/Decarboxylase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002986">
        <property type="entry name" value="DAP_deCOOHase_LysA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022643">
        <property type="entry name" value="De-COase2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022657">
        <property type="entry name" value="De-COase2_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022644">
        <property type="entry name" value="De-COase2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022653">
        <property type="entry name" value="De-COase2_pyr-phos_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000183">
        <property type="entry name" value="Orn/DAP/Arg_de-COase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029066">
        <property type="entry name" value="PLP-binding_barrel"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02784">
        <property type="entry name" value="Orn_Arg_deC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00278">
        <property type="entry name" value="Orn_DAP_Arg_deC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01181">
        <property type="entry name" value="DAPDCRBXLASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01179">
        <property type="entry name" value="ODADCRBXLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50621">
        <property type="entry name" value="SSF50621"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51419">
        <property type="entry name" value="SSF51419"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01048">
        <property type="entry name" value="lysA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00878">
        <property type="entry name" value="ODR_DC_2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00879">
        <property type="entry name" value="ODR_DC_2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="2 3" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="2 3 6" id="KW-0456">Lyase</keyword>
    <keyword evidence="2 3" id="KW-0457">Lysine biosynthesis</keyword>
    <keyword evidence="2" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Orn_Arg_deC_N" evidence="5">
        <location>
            <begin position="51"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="domain" description="Orn_DAP_Arg_deC" evidence="4">
        <location>
            <begin position="299"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="region of interest" description="Pyridoxal phosphate binding" evidence="2">
        <location>
            <begin position="291"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="251"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="294"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="330"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="334"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="361"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="389"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="2">
        <location>
            <position position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02784"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEF95938.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="436" mass="48179" checksum="6217018D7B9A9651" modified="2011-07-27" version="1">MFLGNDMITIENGRLKIDGHDAVELAEKFGTPLYVMSETQIKRNYEKYVDAFKIYEKETGKEFIVAYAYKANANLAVTKLLSKLGCGADVVSGGELYIAKLSGVPSNKIVFNGNCKTKEEIIMGIETNIRAFNVDSISELILINETAKEMGEVANVAFRINPNVDPKTHPKISTGLKKNKFGLDVESGIAMKAIKMALEMENVNVVGVHCHIGSQLTDISPFVEETRKVMDFVVELKKEGIEIEDVNLGGGLGVPYYKDKEIPTQYDLADAIINTMLEYQDKVEMPNLILEPGRSLVATAGVLLGKVHHIKETPVAKWIMIDAGMNDMMRPAIYEAYHEITPCVIRDGEKEKVNIAGGLCESSDVMGKDRELTPIEVGDVVAILDVGAYGISMANNYNARGRPRMVLTNEKGVYLIRERETYADLIAKDIVPPHLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>F6BB68</accession>
    <name>F6BB68_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_0397</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95953.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798562.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0397"/>
    <dbReference type="EnsemblBacteria" id="AEF95953">
        <property type="protein sequence ID" value="AEF95953"/>
        <property type="gene ID" value="Metig_0397"/>
    </dbReference>
    <dbReference type="GeneID" id="10643234"/>
    <dbReference type="KEGG" id="mig:Metig_0397"/>
    <dbReference type="HOGENOM" id="CLU_135994_0_0_2"/>
    <dbReference type="OMA" id="AEHASTF"/>
    <dbReference type="OrthoDB" id="112766at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.630">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007171">
        <property type="entry name" value="DUF371"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023131">
        <property type="entry name" value="Mth639-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40696">
        <property type="entry name" value="PTHR40696"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04027">
        <property type="entry name" value="DUF371"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF95953.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="136" mass="15539" checksum="AA5A20A1D0486438" modified="2011-07-27" version="1">MEFVIHAKGHENVRALHKTTLEITKETYLTPRGDCIIGVSADKSMQDFPEEFKEKIRKSKRIIVEIEVEGIKDVVIGRGDERLILSHPTDIVIRKSDFICPRTLMIKANKGAKDINREIVEKLKNGANLTFRIIVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>F6BB87</accession>
    <name>F6BB87_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">1-(5-phosphoribosyl)-5-amino-4-imidazole-carboxylate (AIR) carboxylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0416</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95972.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798581.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0416"/>
    <dbReference type="EnsemblBacteria" id="AEF95972">
        <property type="protein sequence ID" value="AEF95972"/>
        <property type="gene ID" value="Metig_0416"/>
    </dbReference>
    <dbReference type="GeneID" id="10643253"/>
    <dbReference type="KEGG" id="mig:Metig_0416"/>
    <dbReference type="HOGENOM" id="CLU_065705_0_0_2"/>
    <dbReference type="OMA" id="EVIWGPG"/>
    <dbReference type="OrthoDB" id="73714at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039476">
        <property type="entry name" value="P2CMN_synthase_LarB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000031">
        <property type="entry name" value="PurE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43064">
        <property type="entry name" value="PTHR43064"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00731">
        <property type="entry name" value="AIRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01001">
        <property type="entry name" value="AIRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AIRC" evidence="1">
        <location>
            <begin position="121"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM01001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF95972.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="254" mass="27803" checksum="A3FC47223522E895" modified="2011-07-27" version="1">MRDILIKLKNGEIDIDEAEKRLKLSYFEELGEFCKLDVNRKHRTGIPEAIYGESKNIKDLAEIMVCLAEKNNVAFATRVRDIDALKKELEKYDLENFNLRINETARTFALIKKGHKIEKIGKIGILAAGTSDIPVAEEAKETAEIMGCEVIKSYDVGIAGVHRLFKPLKKMIEEDVCCIIVVAGMEGALPSLVTSLVDVPVIGVPTSVGYGVKITPILTMLHSCSPGLAIVNIDNGFGAGAFAGLIASMIYQKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBA6</accession>
    <name>F6BBA6_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Cnd1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1580</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97113.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF97113">
        <property type="protein sequence ID" value="AEF97113"/>
        <property type="gene ID" value="Metig_1580"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_1580"/>
    <dbReference type="HOGENOM" id="CLU_294355_0_0_2"/>
    <dbReference type="OMA" id="YACNFED"/>
    <dbReference type="OrthoDB" id="19064at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032682">
        <property type="entry name" value="Cnd1_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12717">
        <property type="entry name" value="Cnd1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="SSF48371"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cnd1" evidence="1">
        <location>
            <begin position="893"/>
            <end position="1040"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF97113.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="1042" mass="122380" checksum="EEC6057AC99D2F9A" modified="2011-07-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2020-06-17" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBA8</accession>
    <name>F6BBA8_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_1582</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97115.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF97115">
        <property type="protein sequence ID" value="AEF97115"/>
        <property type="gene ID" value="Metig_1582"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_1582"/>
    <dbReference type="HOGENOM" id="CLU_3194505_0_0_2"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF97115.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="45" mass="4986" checksum="C1B66586E061855C" modified="2011-07-27" version="1">MMPLMKVDTGDLRRRTLLSVKPMGGVGVLPERSDGNLKTVRFLCP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBC6</accession>
    <name>F6BBC6_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Dolichyl-phosphate beta-D-mannosyltransferase</fullName>
            <ecNumber evidence="9">2.4.1.83</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Metig_1600</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GtrA family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.83" evidence="9"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97133.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799726.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1600"/>
    <dbReference type="EnsemblBacteria" id="AEF97133">
        <property type="protein sequence ID" value="AEF97133"/>
        <property type="gene ID" value="Metig_1600"/>
    </dbReference>
    <dbReference type="GeneID" id="10644473"/>
    <dbReference type="KEGG" id="mig:Metig_1600"/>
    <dbReference type="HOGENOM" id="CLU_039727_0_0_2"/>
    <dbReference type="OMA" id="TAYIHGF"/>
    <dbReference type="OrthoDB" id="96698at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004582">
        <property type="term" value="F:dolichyl-phosphate beta-D-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000271">
        <property type="term" value="P:polysaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06442">
        <property type="entry name" value="DPM1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039528">
        <property type="entry name" value="DPM1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007267">
        <property type="entry name" value="GtrA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43398">
        <property type="entry name" value="PTHR43398"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04138">
        <property type="entry name" value="GtrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="229"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="257"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="296"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="321"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="domain" description="Glyco_trans_2-like" evidence="7">
        <location>
            <begin position="4"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="domain" description="GtrA" evidence="8">
        <location>
            <begin position="231"/>
            <end position="345"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AEF97133.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="352" mass="39329" checksum="C32F8FECA79F427F" modified="2011-07-27" version="1">MKVSIVVPTYNEAENIKKLIPLLDSVLKDYNHEIIIVDDNSPDGTAEVAKKLTEKYPVKVIVREKKLGLASAILEGIKSARAELVVVMDADLQHPPEYVLELLKNSNGYDIVIASRYVKGGEIEGWSIFRKVISKGASLLAKIMLPKIRKIKDPESGFFLVRRSKILAIADKINPTGFKFLLEVLCVGDFKVKEIPYTFRPREHGKSKFNFKEVINYIKLLLRLSDYRVIKFGIVGATGIVVNEGLLYILVLLGCPLYIASLVAIESSILSNFILNDLWTFKDRKEGSFISRCLKYHGAVALGAVVNFITLLALVSLGVHYLIANLIGISLGFVVNYIGSEVVVWSRWGRNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-09-29" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBD0</accession>
    <name>F6BBD0_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1604</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97137.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799730.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF97137">
        <property type="protein sequence ID" value="AEF97137"/>
        <property type="gene ID" value="Metig_1604"/>
    </dbReference>
    <dbReference type="GeneID" id="10644477"/>
    <dbReference type="KEGG" id="mig:Metig_1604"/>
    <dbReference type="HOGENOM" id="CLU_1412371_0_0_2"/>
    <dbReference type="OMA" id="IAIMIHY"/>
    <dbReference type="OrthoDB" id="131225at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="44"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="81"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="111"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="145"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="169"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF97137.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="192" mass="22301" checksum="AD496957782BDF7B" modified="2011-07-27" version="1">MEDKISEYREIVLQTFVMLFIIYVFILIYKHYNPYALPGVEKRFLYLLIILGVIVIIFYIQKLNKLLNFIINTSNKIFKPIISLSVGQKFVLLAIIFLITSAIDLALSKEYLANVDAMIAYYFLVLGVLNLLFEYGSESFPKLRTCLSLIILSILIYYTPQITKLYPKAYLIPIIIVIFILILSKIKIKLIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBD9</accession>
    <name>F6BBD9_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(NiFe)-hydrogenase-3-type complex Eha, membrane protein EhaE</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1613</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97146.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799739.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1613"/>
    <dbReference type="EnsemblBacteria" id="AEF97146">
        <property type="protein sequence ID" value="AEF97146"/>
        <property type="gene ID" value="Metig_1613"/>
    </dbReference>
    <dbReference type="GeneID" id="10644486"/>
    <dbReference type="KEGG" id="mig:Metig_1613"/>
    <dbReference type="HOGENOM" id="CLU_191930_0_0_2"/>
    <dbReference type="OMA" id="LETQIWF"/>
    <dbReference type="OrthoDB" id="114920at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011317">
        <property type="entry name" value="Prd_NiFe_hyd_3_EhaE"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09880">
        <property type="entry name" value="DUF2107"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036535">
        <property type="entry name" value="EhaE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="36"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="58"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF97146.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="87" mass="9635" checksum="EC72BCD3EF95E230" modified="2011-07-27" version="1">MDLITLIMYLGYFLLVVGTIGVVFGPMTDDPFKRLLNIEVPSMGVALIFLAYNETLALMTFLGVNAILILVLVRAIVINTELSEEQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBF4</accession>
    <name>F6BBF4_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">4Fe-4S ferredoxin iron-sulfur binding domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1628</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97161.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799753.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1628"/>
    <dbReference type="EnsemblBacteria" id="AEF97161">
        <property type="protein sequence ID" value="AEF97161"/>
        <property type="gene ID" value="Metig_1628"/>
    </dbReference>
    <dbReference type="GeneID" id="10644501"/>
    <dbReference type="KEGG" id="mig:Metig_1628"/>
    <dbReference type="HOGENOM" id="CLU_097041_0_0_2"/>
    <dbReference type="OMA" id="CVKCEIC"/>
    <dbReference type="OrthoDB" id="93710at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00037">
        <property type="entry name" value="Fer4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12838">
        <property type="entry name" value="Fer4_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="39"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="69"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="124"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="154"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="191"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="220"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF97161.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="252" mass="28360" checksum="795DFEF30996E268" modified="2011-07-27" version="1">MITIKKSFKDLISDLGEKFEMDHEKVEEVLKNIKPIKEKTIFINPDKCVRCNLCVEECPVDAIKKPTIKRPAEITDKCVKCEICAQTCPVNAIEVIEGRAYAEKDHIVYELKELSVPHRKLRLKNYKFDRDKCVFCGICAKFCPTNAIEVEIGKNFEVDLNFCMGCGACAYVCPKDAIEVEKELGGVIFNKIISVNNDVCVGCLACIEVCPVNAIKEIPEGVEIDKEKCIFCGRCRDVCPVNAIKIEKINKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBJ1</accession>
    <name>F6BBJ1_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Methyl-coenzyme M reductase subunit gamma</fullName>
            <ecNumber evidence="8">2.8.4.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Metig_0444</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Component of the methyl-coenzyme M reductase (MCR) I that catalyzes the reductive cleavage of methyl-coenzyme M (CoM-S-CH3 or 2-(methylthio)ethanesulfonate) using coenzyme B (CoB or 7-mercaptoheptanoylthreonine phosphate) as reductant which results in the production of methane and the mixed heterodisulfide of CoB and CoM (CoM-S-S-CoB). This is the final step in methanogenesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>coenzyme B + methyl-coenzyme M = coenzyme M-coenzyme B heterodisulfide + methane</text>
            <dbReference type="Rhea" id="RHEA:12532"/>
            <dbReference type="ChEBI" id="CHEBI:16183"/>
            <dbReference type="ChEBI" id="CHEBI:58286"/>
            <dbReference type="ChEBI" id="CHEBI:58411"/>
            <dbReference type="ChEBI" id="CHEBI:58596"/>
            <dbReference type="EC" id="2.8.4.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="1">
            <dbReference type="Rhea" id="RHEA:12533"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>coenzyme F430</name>
            <dbReference type="ChEBI" id="CHEBI:60540"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4 8">One-carbon metabolism; methyl-coenzyme M reduction; methane from methyl-coenzyme M: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Hexamer of two alpha, two beta, and two gamma chains.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">MCR is a hexamer of two alpha, two beta, and two gamma chains, forming a dimer of heterotrimers.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the methyl-coenzyme M reductase gamma subunit family.</text>
    </comment>
    <dbReference type="EC" id="2.8.4.1" evidence="8"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96000.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798609.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0444"/>
    <dbReference type="EnsemblBacteria" id="AEF96000">
        <property type="protein sequence ID" value="AEF96000"/>
        <property type="gene ID" value="Metig_0444"/>
    </dbReference>
    <dbReference type="GeneID" id="10643281"/>
    <dbReference type="KEGG" id="mig:Metig_0444"/>
    <dbReference type="HOGENOM" id="CLU_1092436_0_0_2"/>
    <dbReference type="OMA" id="GHRNPGE"/>
    <dbReference type="OrthoDB" id="51032at2157"/>
    <dbReference type="UniPathway" id="UPA00646">
        <property type="reaction ID" value="UER00699"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050524">
        <property type="term" value="F:coenzyme-B sulfoethylthiotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015948">
        <property type="term" value="P:methanogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00539">
        <property type="entry name" value="MCR_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.320.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009024">
        <property type="entry name" value="Me_CoM_Rdtase_Fd-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003178">
        <property type="entry name" value="Me_CoM_Rdtase_gsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036994">
        <property type="entry name" value="Me_CoM_Rdtase_gsu_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02240">
        <property type="entry name" value="MCR_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000264">
        <property type="entry name" value="Meth_CoM_rd_gama"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55088">
        <property type="entry name" value="SSF55088"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03259">
        <property type="entry name" value="met_CoM_red_gam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0484">Methanogenesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 9" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000951"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00002461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00008740"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00011155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR000264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AEF96000.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="265" mass="30739" checksum="193EBDBA75A46429" modified="2011-07-27" version="1">MAYKPQFYPGETLIAENRRKHMNPNYELKKLREISDDDLVRVLGHRNPGESYKTVHPPLEEMDFEEDVIKEIVEPIPGAKEGIRVRYIQFADSMYNAPAQPYDRARTYMWRFRGVDTGTLSGRQVIEIRELDLERISKWLVETEIFDPATCGMRGATVHGHSLRLDENGLMFDGLQRYIYDEKTGHVIYVKDQVGRPLDEPVDVGEPLPQEYLAKITTIYRKDNIGMREDKEALEVVEIIHIARTEGGFGLNVFKKDLKRRLGEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBJ7</accession>
    <name>F6BBJ7_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Polyamine-transporting ATPase</fullName>
            <ecNumber evidence="5">3.6.3.31</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Metig_0450</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.6.3.31" evidence="5"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96006.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798615.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0450"/>
    <dbReference type="EnsemblBacteria" id="AEF96006">
        <property type="protein sequence ID" value="AEF96006"/>
        <property type="gene ID" value="Metig_0450"/>
    </dbReference>
    <dbReference type="GeneID" id="10643287"/>
    <dbReference type="KEGG" id="mig:Metig_0450"/>
    <dbReference type="HOGENOM" id="CLU_000604_1_1_2"/>
    <dbReference type="OMA" id="HFIILGP"/>
    <dbReference type="OrthoDB" id="88924at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="2"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="32"/>
            <end position="39"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEF96006.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="312" mass="35344" checksum="68BDE15FA1A5EE85" modified="2011-07-27" version="1">MLRIENLSKIWKEFQLKNINLEINKEYCIILGPSGAGKSVLLKCIAGILKVDSGKIYFNGEDITNLPPEKRNFGYVPQNYALFPNMNVYKNIAYGLRIRGYPKPKIDKKVEEIAEFLNIKHLLNRKPTTLSGGEQQRVALARALVLEPNLLLLDEPTSALDMNNKEKIIGELKRVGEILPIIHITHDFVEAKTLGEKIAIFMDGKLVEVGDREIFKKPKNERVAKFLGYNIVKINGEKFAIAPEDVVICRGSKGKIVNIIDCGFYKKVMVDFEGNILRCILKEDKEDIDLNNLNIGDRVGVKFENKIYLVGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBN5</accession>
    <name>F6BBN5_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Signal recognition particle receptor FtsY</fullName>
            <shortName evidence="4">SRP receptor</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">ftsY</name>
        <name evidence="8" type="ordered locus">Metig_0488</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Involved in targeting and insertion of nascent membrane proteins into the cytoplasmic membrane. Acts as a receptor for the complex formed by the signal recognition particle (SRP) and the ribosome-nascent chain (RNC).</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Part of the signal recognition particle protein translocation system, which is composed of SRP and FtsY.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell membrane</location>
            <topology evidence="4">Peripheral membrane protein</topology>
            <orientation evidence="4">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the GTP-binding SRP family. FtsY subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96044.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0488"/>
    <dbReference type="EnsemblBacteria" id="AEF96044">
        <property type="protein sequence ID" value="AEF96044"/>
        <property type="gene ID" value="Metig_0488"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0488"/>
    <dbReference type="HOGENOM" id="CLU_009301_3_1_2"/>
    <dbReference type="OMA" id="GISDQFQ"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031226">
        <property type="term" value="C:intrinsic component of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006614">
        <property type="term" value="P:SRP-dependent cotranslational protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00920">
        <property type="entry name" value="FtsY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013822">
        <property type="entry name" value="Signal_recog_particl_SRP54_hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004390">
        <property type="entry name" value="SR_rcpt_FtsY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036225">
        <property type="entry name" value="SRP/SRP_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000897">
        <property type="entry name" value="SRP54_GTPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042101">
        <property type="entry name" value="SRP54_N_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00448">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02881">
        <property type="entry name" value="SRP54_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00962">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00963">
        <property type="entry name" value="SRP54_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47364">
        <property type="entry name" value="SSF47364"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00064">
        <property type="entry name" value="ftsY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00300">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="1 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3 4" id="KW-0675">Receptor</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SRP54" evidence="7">
        <location>
            <begin position="360"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="32"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="99"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="199"/>
            <end position="206"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="281"/>
            <end position="285"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="339"/>
            <end position="342"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS00300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEF96044.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="399" mass="44493" checksum="051CEBF0085B1667" modified="2011-07-27" version="1">MVTIMFGKLKEKLGKTISKLTEKIYKKGEAKEVTIEQSKEEKPQTQMEKSEKKSPFGFLKRDTPTKDKEKEKTIEKIEETPQKKEISFFDRFKITKTIKKVLKKEVVITEDDIEDVLEELELELLEADVAYVVAEKIIESLKNQLVGMKISPNDDPEEITINALKNAIKEILSQEHIDVEEIIKEKKKKGEPAVFVFVGINGTGKTTSIAKLAYKLKNKGYSVVLAAGDTFRAGAIEQLEEHAKNVGVKVIKHQKGADSAAVIYDAIQHAKARGIDVVLADTAGRQATNVNLMDEIKKVVRVTKPDLVIFVGDALAGNDAIYQAEEFNNAVNIDGVILTKVDADAKGGAALSIAYAIGKPILYLGVGQRYEDLIEFDVDWMVNKLFGEEEVKFSTEREF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBS4</accession>
    <name>F6BBS4_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Microtubule-severing ATPase</fullName>
            <ecNumber evidence="4">3.6.4.3</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metig_1672</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AAA ATPase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.3" evidence="4"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97204.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799795.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1672"/>
    <dbReference type="EnsemblBacteria" id="AEF97204">
        <property type="protein sequence ID" value="AEF97204"/>
        <property type="gene ID" value="Metig_1672"/>
    </dbReference>
    <dbReference type="GeneID" id="10644545"/>
    <dbReference type="KEGG" id="mig:Metig_1672"/>
    <dbReference type="HOGENOM" id="CLU_000688_21_2_2"/>
    <dbReference type="OMA" id="ATIMSKW"/>
    <dbReference type="OrthoDB" id="44676at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015415">
        <property type="entry name" value="Vps4_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09336">
        <property type="entry name" value="Vps4_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA" evidence="3">
        <location>
            <begin position="149"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="74"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="80"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU003651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEF97204.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="397" mass="45146" checksum="FE8C09D00D026C27" modified="2011-07-27" version="1">MEIDLSGALMSQFKKAKKEYEMAKEKNNLDIAKKKALECAKLLRLMAKYDEYNEKSYLEKAKKWEMVANSISNPSKVNKSKPAKKSESSNTTKAEVEDEIDKFKNFVKNNLIQKSPITWEDIGGLKEVKQLMMETIVISALQKPASIQPWKGILLFGPPGTGKTLLASASAGSLEATFFNVKASSVLSKYYGESSKIISALYDVAREMAPSIVFIDEIDALTTKRSEETSEASRRMLSTLLTELDGFQDKGRDRLILTLAATNTPWDLDEAILSRFSRRIYIPLPDKEATKEIIKINTKGVKLNVNLDEIADKCVERFYSGRDLKNLCQEAIWNMIRDVNKDLHELAKLPYNELRKRKLNVRPLTNDDFEEAFKKIKSPLTKKDIEKYEKWAEEFGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBT2</accession>
    <name>F6BBT2_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0200 protein Metig_1680</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1680</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0200 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97212.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799803.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1680"/>
    <dbReference type="EnsemblBacteria" id="AEF97212">
        <property type="protein sequence ID" value="AEF97212"/>
        <property type="gene ID" value="Metig_1680"/>
    </dbReference>
    <dbReference type="GeneID" id="10644553"/>
    <dbReference type="KEGG" id="mig:Metig_1680"/>
    <dbReference type="HOGENOM" id="CLU_096329_1_0_2"/>
    <dbReference type="OMA" id="DYMIVNE"/>
    <dbReference type="OrthoDB" id="101213at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01111">
        <property type="entry name" value="UPF0200"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022970">
        <property type="entry name" value="NTP_hydrolase-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="10"/>
            <end position="17"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF97212.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="187" mass="21664" checksum="1EC20F4A8A306CC8" modified="2011-07-27" version="1">MVEVLIGITGMPGSGKSAIKEVAKKFNIPVVSMGDVVREETRKKGLELTPENVGNMAIKLREMYGKEAIAVPCLKYIEKYFANYDFVIIEGVRSLYEVNYFKKYYPFLTIAIHASPKTRFERLMKRKREDDTMDWEEFVKRDMRELGFSIGGVIALADYMIVNEGDYKSYLQELERTLKKIIDSFKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-06-02" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBY9</accession>
    <name>F6BBY9_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_0514</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96070.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798678.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96070">
        <property type="protein sequence ID" value="AEF96070"/>
        <property type="gene ID" value="Metig_0514"/>
    </dbReference>
    <dbReference type="GeneID" id="10643351"/>
    <dbReference type="KEGG" id="mig:Metig_0514"/>
    <dbReference type="HOGENOM" id="CLU_2968474_0_0_2"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF96070.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="62" mass="7043" checksum="57CCD9AB0C6B7C02" modified="2011-07-27" version="1">MEVEGLLSRLVNEVERAKSDYHNGDEIGFLTHLTNAEGLIREIITLHNIMKGEKGYDAIFIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F6BC09</accession>
    <name>F6BC09_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Xanthine/uracil/vitamin C permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Metig_0535</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the nucleobase:cation symporter-2 (NCS2) (TC 2.A.40) family. Azg-like subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96090.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798697.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0535"/>
    <dbReference type="EnsemblBacteria" id="AEF96090">
        <property type="protein sequence ID" value="AEF96090"/>
        <property type="gene ID" value="Metig_0535"/>
    </dbReference>
    <dbReference type="GeneID" id="10643373"/>
    <dbReference type="KEGG" id="mig:Metig_0535"/>
    <dbReference type="HOGENOM" id="CLU_024508_0_1_2"/>
    <dbReference type="OMA" id="RKGSYFF"/>
    <dbReference type="OrthoDB" id="29114at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015205">
        <property type="term" value="F:nucleobase transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904823">
        <property type="term" value="P:purine nucleobase transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045018">
        <property type="entry name" value="Azg-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026033">
        <property type="entry name" value="Azg-like_bact_archaea"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006043">
        <property type="entry name" value="NCS2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43337">
        <property type="entry name" value="PTHR43337"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00860">
        <property type="entry name" value="Xan_ur_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005353">
        <property type="entry name" value="PbuG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="20"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="50"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="78"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="103"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="134"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="170"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="196"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="236"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="319"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="347"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="378"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="416"/>
            <end position="433"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEF96090.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="434" mass="46312" checksum="A8D2635D128C1736" modified="2011-07-27" version="1">MLSKIAEYFEFEKYKTDFRVEVLAGITTFMTMAYIIFVNPMILSNAGMDFGAVMVATCISSAIATLLMGVFARYPFALAPGMGLNAYFTYGVCLGMGVDWRVALGAVFISGILFVILTLTKIRTMIFNAIPNAIKYGTAVGIGLFIAFIGLKSAGIIVDSEATLVTLGNLLEPSTLLAMFGIFLTGILLCRKVVGAILWGIIITALIGMILGISPFPEGIVSMPPSIAPTFMQLDIMGALNLGLLTIVLSFFFVDLFDTLGTLSALSSQAGYLKDGKLPRSERALMSDSIGTVLGSILGTSTVTTYIESAAGIAVGGRTGFVSIIVALLFLLALFFYPIVKAIPAYATAPALVIVGALMMTCVKYIDWDDVSEAIPAFITLISIPLTFSIATGLALGFITYPILKVFSGKAKEVHWLVYILAVVFALRFVYLSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>F6BC33</accession>
    <name>F6BC33_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Aspartate--tRNA(Asp/Asn) ligase</fullName>
            <ecNumber evidence="7">6.1.1.23</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Aspartyl-tRNA synthetase</fullName>
            <shortName evidence="7">AspRS</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Non-discriminating aspartyl-tRNA synthetase</fullName>
            <shortName evidence="7">ND-AspRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">aspS</name>
        <name evidence="9" type="ordered locus">Metig_0560</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Aspartyl-tRNA synthetase with relaxed tRNA specificity since it is able to aspartylate not only its cognate tRNA(Asp) but also tRNA(Asn). Reaction proceeds in two steps: L-aspartate is first activated by ATP to form Asp-AMP and then transferred to the acceptor end of tRNA(Asp/Asn).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>ATP + L-aspartate + tRNA(Asx) = AMP + diphosphate + L-aspartyl-tRNA(Asx)</text>
            <dbReference type="Rhea" id="RHEA:18349"/>
            <dbReference type="Rhea" id="RHEA-COMP:9710"/>
            <dbReference type="Rhea" id="RHEA-COMP:9711"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78516"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.23"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="7">Binds 3 Mg(2+) cations per subunit. The strongest magnesium site (Mg1) is bound to the beta- and gamma-phosphates of ATP and four water molecules complete its coordination sphere.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the class-II aminoacyl-tRNA synthetase family. Type 2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.23" evidence="7"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96114.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0560"/>
    <dbReference type="EnsemblBacteria" id="AEF96114">
        <property type="protein sequence ID" value="AEF96114"/>
        <property type="gene ID" value="Metig_0560"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0560"/>
    <dbReference type="HOGENOM" id="CLU_004553_2_1_2"/>
    <dbReference type="OMA" id="WVHEIRD"/>
    <dbReference type="OrthoDB" id="24288at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004815">
        <property type="term" value="F:aspartate-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050560">
        <property type="term" value="F:aspartate-tRNA(Asn) ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006422">
        <property type="term" value="P:aspartyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02075">
        <property type="entry name" value="Asp_tRNA_synth_type2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004364">
        <property type="entry name" value="Aa-tRNA-synt_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004523">
        <property type="entry name" value="Asp-tRNA_synthase_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002312">
        <property type="entry name" value="Asp/Asn-tRNA-synth_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004365">
        <property type="entry name" value="NA-bd_OB_tRNA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43450">
        <property type="entry name" value="PTHR43450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00152">
        <property type="entry name" value="tRNA-synt_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01336">
        <property type="entry name" value="tRNA_anti-codon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01042">
        <property type="entry name" value="TRNASYNTHASP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="SSF55681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00458">
        <property type="entry name" value="aspS_nondisc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7 9" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="5 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 7 9" id="KW-0436">Ligase</keyword>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AA_TRNA_LIGASE_II" evidence="8">
        <location>
            <begin position="141"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="region of interest" description="Aspartate" evidence="7">
        <location>
            <begin position="196"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="174"/>
        </location>
        <ligand>
            <name>L-aspartate</name>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="218"/>
            <end position="220"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="218"/>
        </location>
        <ligand>
            <name>L-aspartate</name>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="226"/>
            <end position="228"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="359"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="362"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="366"/>
        </location>
        <ligand>
            <name>L-aspartate</name>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="407"/>
            <end position="410"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="site" description="Important for tRNA non-discrimination" evidence="7">
        <location>
            <position position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AEF96114.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="436" mass="49884" checksum="B87424A30CDA4519" modified="2011-07-27" version="1">MLGDWRRTHYSSQISPEMDGEEVIVMGWAHAIRKLGKLIFIILRDREGTVQIVVPKQKVEEEIFKTAKEIGKEDVIAIKGKVIANEKAPNGFEILPIEIRILNKAATPLPLDPAEKVSADIDTRLDNRFLDLRRPKIQAIFKIRSEMLKSVRKTFYENGFVEVNTPKLVASCTEGGTELFPISYFEKEAFLGQSPQLYKQMLMATGLDRVFEIGPIFRAEEHNTRRHLNEAISIDVEMAFCDDKDAMEMLEKVVYNAFVDVYENRKKELELLGVNLELPNEKFDRITYDEAIDIANAKGVELEWGDDLSRAAEKAIGEEMGGLYFIVDWPTETRPFYTMPNEKDPKICKAFDLMYKDLEISSGAQRIHIYDLLVERIKAQGLNPEGFGSYLEAFKYGMPPHAGWGLGADRFTMVLTGQENIRECVMFPRDRQRLTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-06-02" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>F6BC68</accession>
    <name>F6BC68_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_1742</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97274.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799863.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF97274">
        <property type="protein sequence ID" value="AEF97274"/>
        <property type="gene ID" value="Metig_1742"/>
    </dbReference>
    <dbReference type="GeneID" id="10644616"/>
    <dbReference type="KEGG" id="mig:Metig_1742"/>
    <dbReference type="HOGENOM" id="CLU_2392906_0_0_2"/>
    <dbReference type="OMA" id="IMVYNSY"/>
    <dbReference type="OrthoDB" id="128665at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF97274.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="93" mass="10710" checksum="9F853FD6EDBF688D" modified="2011-07-27" version="1">MCQGNIGIFRGFVYKNGYKIPLIEINGNISEIPLPITKDVKFGDRIMVYNSYNFEYEFEPLELKHKLLDVISAIKEKLSKLRALNSHAVYLGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>F6BC84</accession>
    <name>F6BC84_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Probable GTP 3',8-cyclase</fullName>
            <ecNumber evidence="9">4.1.99.22</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Molybdenum cofactor biosynthesis protein A</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">moaA</name>
        <name evidence="11" type="ordered locus">Metig_1758</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the cyclization of GTP to (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>AH2 + GTP + S-adenosyl-L-methionine = (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate + 5'-deoxyadenosine + A + H(+) + L-methionine</text>
            <dbReference type="Rhea" id="RHEA:49576"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:131766"/>
            <dbReference type="EC" id="4.1.99.22"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="9">Binds 2 [4Fe-4S] clusters. Binds 1 [4Fe-4S] cluster coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine and 1 [4Fe-4S] cluster coordinated with 3 cysteines and the GTP-derived substrate.</text>
    </comment>
    <comment type="pathway">
        <text evidence="9">Cofactor biosynthesis; molybdopterin biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the radical SAM superfamily. MoaA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="4.1.99.22" evidence="9"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97290.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799879.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1758"/>
    <dbReference type="EnsemblBacteria" id="AEF97290">
        <property type="protein sequence ID" value="AEF97290"/>
        <property type="gene ID" value="Metig_1758"/>
    </dbReference>
    <dbReference type="GeneID" id="10644632"/>
    <dbReference type="KEGG" id="mig:Metig_1758"/>
    <dbReference type="HOGENOM" id="CLU_009273_0_1_2"/>
    <dbReference type="OMA" id="IEFMPIG"/>
    <dbReference type="OrthoDB" id="18533at2157"/>
    <dbReference type="UniPathway" id="UPA00344"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019008">
        <property type="term" value="C:molybdopterin synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061798">
        <property type="term" value="F:GTP 3',8'-cyclase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904047">
        <property type="term" value="F:S-adenosyl-L-methionine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006777">
        <property type="term" value="P:Mo-molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01225_A">
        <property type="entry name" value="MoaA_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaB/NifB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013485">
        <property type="entry name" value="MoaA_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010505">
        <property type="entry name" value="Mob_synth_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06463">
        <property type="entry name" value="Mob_synth_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02668">
        <property type="entry name" value="moaA_archaeal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="6 9" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4 9" id="KW-0408">Iron</keyword>
    <keyword evidence="5 9" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="8 9" id="KW-0456">Lyase</keyword>
    <keyword evidence="2 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 9" id="KW-0501">Molybdenum cofactor biosynthesis</keyword>
    <keyword evidence="3 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 9" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="10">
        <location>
            <begin position="4"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="13"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="20"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="24"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="26"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="27"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="61"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="65"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="91"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="115"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="152"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="243"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
            <note>4Fe-4S-substrate</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="246"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
            <note>4Fe-4S-substrate</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="248"/>
            <end position="250"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="260"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
            <note>4Fe-4S-substrate</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AEF97290.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="301" mass="35117" checksum="FC8EF3CE04E27D31" modified="2011-07-27" version="1">MKDKFGREIRSLRISVTQKCNLKCFYCHKEGRKENTNRSMLPEEIGKIVKTSLDFGVKKIKISGGEPLLRKDITEILKNIRDKRIKDTSMTTNGILLEEYAEKLKDNGLDRVNVSLDTLNNELYKKITKYGDVEKVKRGIEKAIDVGLTPLKINYLAMDINIDHLKDIMEYCREIGAILQIIEFIPTNKNLNKHHVDITPIENKIKEKADKIITRKFMQNRKKYVLDGLEIEFVRPMDNTEFCSHCTRIRLTHDGFLKPCLLRDDNLVDVLTPLRKGEDIRRCFVECINRREPYFKGKDAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>F6BC89</accession>
    <name>F6BC89_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">SagB-type dehydrogenase domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1763</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97295.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799884.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1763"/>
    <dbReference type="EnsemblBacteria" id="AEF97295">
        <property type="protein sequence ID" value="AEF97295"/>
        <property type="gene ID" value="Metig_1763"/>
    </dbReference>
    <dbReference type="GeneID" id="10644637"/>
    <dbReference type="KEGG" id="mig:Metig_1763"/>
    <dbReference type="HOGENOM" id="CLU_059362_3_1_2"/>
    <dbReference type="OMA" id="CAIAAYD"/>
    <dbReference type="OrthoDB" id="107170at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.109.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029479">
        <property type="entry name" value="Nitroreductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000415">
        <property type="entry name" value="Nitroreductase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020051">
        <property type="entry name" value="SagB-type_dehydrogenase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00881">
        <property type="entry name" value="Nitroreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55469">
        <property type="entry name" value="SSF55469"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03605">
        <property type="entry name" value="antibiot_sagB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nitroreductase" evidence="1">
        <location>
            <begin position="19"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF97295.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="193" mass="21593" checksum="04D48B623778A42B" modified="2011-07-27" version="1">MEIKLPNPKLINIEPVLIKRRSIREYKDEPLTLEELSQLLFACQGITDDYGFRTAPSAGATYPLEIYVSVKNVVGLSKGVYKYIPHSHSLLKVFDGDIGEELATLALNQYFIAEAPVVIIFTAVYERATQRYGKRGIRYVHMEVGYASQNVYLEATALNLGTVAVGAFDDEGIMELMGLEEEYPLLLMPVGRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F6BC97</accession>
    <name>F6BC97_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KH domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1771</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97303.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1771"/>
    <dbReference type="EnsemblBacteria" id="AEF97303">
        <property type="protein sequence ID" value="AEF97303"/>
        <property type="gene ID" value="Metig_1771"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_1771"/>
    <dbReference type="HOGENOM" id="CLU_064992_3_0_2"/>
    <dbReference type="OMA" id="DPLAVWK"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004088">
        <property type="entry name" value="KH_dom_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019964">
        <property type="entry name" value="KH_domain_protein_archaea"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039912">
        <property type="entry name" value="PNO1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12826:SF13">
        <property type="entry name" value="PTHR12826:SF13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00013">
        <property type="entry name" value="KH_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="SSF54791"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03665">
        <property type="entry name" value="arCOG04150"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="KH" evidence="1">
        <location>
            <begin position="11"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="domain" description="KH" evidence="1">
        <location>
            <begin position="94"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF97303.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="194" mass="21838" checksum="11B2BC1A52B791A6" modified="2011-07-27" version="1">MAMNFSDVVMHGNVETVKIPKNRIGVLIGKKGQTKKTIERELGVELEISKDGDVTIYSTEKQKDALATWKARDIVMAIGRGFSPENALKLLSDEYVLEIIDITEYASSENALRRLKGRVIGSGGKSRKYIEDLTGARVSVFGKTVAILGEFESVQIAKEAVEMILRGSSHAKMYKFLERERQKIKRKAFELWKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>F6BC99</accession>
    <name>F6BC99_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_1773</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97305.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1773"/>
    <dbReference type="EnsemblBacteria" id="AEF97305">
        <property type="protein sequence ID" value="AEF97305"/>
        <property type="gene ID" value="Metig_1773"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_1773"/>
    <dbReference type="HOGENOM" id="CLU_1521868_0_0_2"/>
    <dbReference type="OMA" id="KCKIYKV"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005358">
        <property type="entry name" value="Puta_zinc/iron-chelating_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03692">
        <property type="entry name" value="CxxCxxCC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF97305.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="187" mass="21942" checksum="9C25566CEF2FDCAF" modified="2011-07-27" version="1">MEKSTSKNENKKTNAKINLKNKNSKILKIMGSYTCGIAWYCKKCGGCCDSPTVTKKDIVNIAKYLGLSFNEVIEKYLRYFDGKMGEIKEVRGRCIFLDKRRCTIYKARPLICKLRPFSPQFKNGKLVLTYDIWFLKHCRGFFIGDMKIDKKYFKYAEILVKSLGFEEETPKEEFNRLKKRLKNAHEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-06-02" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCA5</accession>
    <name>F6BCA5_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Cas_Cas4 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_1779</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97311.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799900.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF97311">
        <property type="protein sequence ID" value="AEF97311"/>
        <property type="gene ID" value="Metig_1779"/>
    </dbReference>
    <dbReference type="GeneID" id="10644655"/>
    <dbReference type="KEGG" id="mig:Metig_1779"/>
    <dbReference type="HOGENOM" id="CLU_1912352_0_0_2"/>
    <dbReference type="OrthoDB" id="107054at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022765">
        <property type="entry name" value="Dna2/Cas4_DUF83"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01930">
        <property type="entry name" value="Cas_Cas4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cas_Cas4" evidence="2">
        <location>
            <begin position="34"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="112"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF97311.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="132" mass="15888" checksum="470A8AABFF1CE13C" modified="2011-07-27" version="1">MFKEFDMYPYVRRNLRKRYPASKGWVIKERERRGSYEPDFIVERRTKSVIERIVVEVKATPKITENHIKQINRYAKCLAGANVKIKEKILVVPSGAKVSKIPPDIKIHYSKKFSHKKKKSWKKRKRKYRKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCB9</accession>
    <name>F6BCB9_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Coenzyme F420 hydrogenase, subunit gamma</fullName>
            <ecNumber evidence="4">1.12.98.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metig_1793</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FrhG family.</text>
    </comment>
    <dbReference type="EC" id="1.12.98.1" evidence="4"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97325.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799914.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1793"/>
    <dbReference type="EnsemblBacteria" id="AEF97325">
        <property type="protein sequence ID" value="AEF97325"/>
        <property type="gene ID" value="Metig_1793"/>
    </dbReference>
    <dbReference type="GeneID" id="10644670"/>
    <dbReference type="KEGG" id="mig:Metig_1793"/>
    <dbReference type="HOGENOM" id="CLU_075477_0_0_2"/>
    <dbReference type="OMA" id="CNAENVH"/>
    <dbReference type="OrthoDB" id="54703at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050454">
        <property type="term" value="F:coenzyme F420 hydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016151">
        <property type="term" value="F:nickel cation binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.700">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017681">
        <property type="entry name" value="Coenz_F420_hydrogenase_gsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006137">
        <property type="entry name" value="NADH_UbQ_OxRdtase-like_20kDa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037024">
        <property type="entry name" value="NiFe_Hase_small_N_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00037">
        <property type="entry name" value="Fer4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01058">
        <property type="entry name" value="Oxidored_q6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03294">
        <property type="entry name" value="FrhG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="3">
        <location>
            <begin position="166"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="3">
        <location>
            <begin position="196"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEF97325.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="227" mass="24401" checksum="1798D62746BD121F" modified="2011-07-27" version="1">MVKVAHVQLSSCCGCLVSLADTYEKLLDILNSIELVYCQTLMDVREIPDNLDIILVEGSVCLDDHHALELAEEVRKKAKILVALGACAATGGVTRFCRGNQMSKPVHSSFSPLTEVVKCDLAIPGCPPSPEVIVNVITAALNGDAEYLQPFAELAEKGEACGCDLLYKVVNKSLCMGCGTCAAACPTRAIEMLDGRPNVLKELCIKCGACSVQCPRIRFPKLIEEIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCE8</accession>
    <name>F6BCE8_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Anion transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Metig_0605</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96159.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96159">
        <property type="protein sequence ID" value="AEF96159"/>
        <property type="gene ID" value="Metig_0605"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0605"/>
    <dbReference type="HOGENOM" id="CLU_005170_0_2_2"/>
    <dbReference type="OMA" id="LHGMNTY"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001898">
        <property type="entry name" value="SLC13A/DASS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00939">
        <property type="entry name" value="Na_sulph_symp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00785">
        <property type="entry name" value="dass"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="47"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="88"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="199"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="242"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="283"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="312"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="343"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="383"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="424"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="463"/>
            <end position="480"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEF96159.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="481" mass="52836" checksum="04B6ED4EB5F53CE7" modified="2011-07-27" version="1">MKSKLLKFKRGIMNKNTLILIFDIILMFLILFFFPIDDLQIKEGLSILVFISILWLTEALPLPISSLFVPVLAVLFGILDVKEAFKSFAHPIIFLFLGGFALASALRKYELDKLIAYKIVGLSKGDFRVASFLIMLTTAFLSMWISNTSATAIMLPLAIGLLGYANNNKIDNNIEFYTSKQSEEVSPRDATTHRCNENIYAFLLLGIAYSASVGGIGTIIGSPPNAITASFLSMGFFDWFKVGFPIVLILLPIIYAVLFLYFKPQFERKISVKYDFKIKNSKALLVGGVFLFVILMWMLSGKLSALFGVEKYFDSIVAVIGIILLFSLRLINWEDLNNSTDWGVLLLFGGALSLSYILSTTGASEYIANIVINALKGVPTFAYIFGLVLFSIMITNIMSNTGVASVLVPILIATASQLNIRPEILALPVGIAVSCAFMLPVATPPNALVFGSGYIKEKDMIKAGFILSLISAVIVTLMFGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCF8</accession>
    <name>F6BCF8_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Peptidase S26B, signal peptidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Metig_0619</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96169.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0619"/>
    <dbReference type="MEROPS" id="S26.017"/>
    <dbReference type="EnsemblBacteria" id="AEF96169">
        <property type="protein sequence ID" value="AEF96169"/>
        <property type="gene ID" value="Metig_0619"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0619"/>
    <dbReference type="HOGENOM" id="CLU_118895_0_0_2"/>
    <dbReference type="OMA" id="SAHWTNA"/>
    <dbReference type="OrthoDB" id="106361at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006465">
        <property type="term" value="P:signal peptide processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06530">
        <property type="entry name" value="S26_SPase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036286">
        <property type="entry name" value="LexA/Signal_pep-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019533">
        <property type="entry name" value="Peptidase_S26"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001733">
        <property type="entry name" value="Peptidase_S26B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027245">
        <property type="entry name" value="Sec11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10806">
        <property type="entry name" value="PTHR10806"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10806:SF6">
        <property type="entry name" value="PTHR10806:SF6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51306">
        <property type="entry name" value="SSF51306"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="156"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEF96169.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="193" mass="22477" checksum="49DF3584BE87B2E7" modified="2011-07-27" version="1">MTSKGEIINKKDIIEWVVFLLVLFLVWSHVNVVVSNSMYPIMERGDLVVVENAHFEFDPNNLNVGDIVVYNAHWPLYQYNEVKGYISVDNKSFFVFEGDKTKPVIHRIIEKLNIDNKTYYVVKGDNNPTYDPELVSPSQIRQKVVTINGKPLVIPYIGYLSIWLKSHVYLVIILLIILYLYDHFRAKNEGEKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCH6</accession>
    <name>F6BCH6_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Probable bifunctional tRNA threonylcarbamoyladenosine biosynthesis protein</fullName>
        </recommendedName>
        <domain>
            <recommendedName>
                <fullName evidence="16">tRNA N6-adenosine threonylcarbamoyltransferase</fullName>
                <ecNumber evidence="16">2.3.1.234</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="16">tRNA threonylcarbamoyladenosine biosynthesis protein Kae1</fullName>
            </alternativeName>
            <alternativeName>
                <fullName evidence="16">t(6)A37 threonylcarbamoyladenosine biosynthesis protein Kae1</fullName>
            </alternativeName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="16">Serine/threonine-protein kinase Bud32</fullName>
                <ecNumber evidence="16">2.7.11.1</ecNumber>
            </recommendedName>
        </domain>
    </protein>
    <gene>
        <name evidence="18" type="ordered locus">Metig_0637</name>
    </gene>
    <organism evidence="19">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="16">Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. Is a component of the KEOPS complex that is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. The Kae1 domain likely plays a direct catalytic role in this reaction. The Bud32 domain probably displays kinase activity that regulates Kae1 function.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>ATP + L-seryl-[protein] = ADP + H(+) + O-phospho-L-seryl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + L-threonyl-[protein] = ADP + H(+) + O-phospho-L-threonyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3 16">
            <text>adenosine(37) in tRNA + L-threonylcarbamoyladenylate = AMP + H(+) + N(6)-L-threonylcarbamoyladenosine(37) in tRNA</text>
            <dbReference type="Rhea" id="RHEA:37059"/>
            <dbReference type="Rhea" id="RHEA-COMP:10162"/>
            <dbReference type="Rhea" id="RHEA-COMP:10163"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:73682"/>
            <dbReference type="ChEBI" id="CHEBI:74411"/>
            <dbReference type="ChEBI" id="CHEBI:74418"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.3.1.234"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="16">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
        <text evidence="16">Binds 1 Fe(2+) ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="16">Component of the KEOPS complex that consists of Kae1, Bud32, Cgi121 and Pcc1; the whole complex dimerizes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="16">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the protein kinase superfamily. BUD32 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="16">In the C-terminal section; belongs to the protein kinase superfamily. Tyr protein kinase family. BUD32 subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="16">In the N-terminal section; belongs to the KAE1 / TsaD family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.234" evidence="16"/>
    <dbReference type="EC" id="2.7.11.1" evidence="16"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96187.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798792.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0637"/>
    <dbReference type="EnsemblBacteria" id="AEF96187">
        <property type="protein sequence ID" value="AEF96187"/>
        <property type="gene ID" value="Metig_0637"/>
    </dbReference>
    <dbReference type="GeneID" id="10643476"/>
    <dbReference type="KEGG" id="mig:Metig_0637"/>
    <dbReference type="HOGENOM" id="CLU_023208_2_2_2"/>
    <dbReference type="OMA" id="GETMDTG"/>
    <dbReference type="OrthoDB" id="9881at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000408">
        <property type="term" value="C:EKC/KEOPS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061711">
        <property type="term" value="F:N(6)-L-threonylcarbamoyladenine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106310">
        <property type="term" value="F:protein serine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004712">
        <property type="term" value="F:protein serine/threonine/tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006468">
        <property type="term" value="P:protein phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002949">
        <property type="term" value="P:tRNA threonylcarbamoyladenosine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01446">
        <property type="entry name" value="Kae1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01447">
        <property type="entry name" value="Kae1_Bud32_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022495">
        <property type="entry name" value="Bud32"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000905">
        <property type="entry name" value="Gcp-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017861">
        <property type="entry name" value="KAE1/TsaD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022449">
        <property type="entry name" value="Kae1_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034680">
        <property type="entry name" value="Kae1_archaea_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017860">
        <property type="entry name" value="Peptidase_M22_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009220">
        <property type="entry name" value="tRNA_threonyl_synthase/kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11735">
        <property type="entry name" value="PTHR11735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11735:SF14">
        <property type="entry name" value="PTHR11735:SF14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00814">
        <property type="entry name" value="TsaD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036401">
        <property type="entry name" value="Gcp_STYKS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00789">
        <property type="entry name" value="OSIALOPTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="SSF53067"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="SSF56112"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03724">
        <property type="entry name" value="arch_bud32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03722">
        <property type="entry name" value="arch_KAE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00329">
        <property type="entry name" value="gcp_kae1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01016">
        <property type="entry name" value="GLYCOPROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15 16" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="12 16" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 16" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="18" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13 16" id="KW-0408">Iron</keyword>
    <keyword evidence="11 16 18" id="KW-0418">Kinase</keyword>
    <keyword evidence="9 16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14 16" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="10 16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 16 18" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="7 16 18" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 16" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="Protein kinase" evidence="17">
        <location>
            <begin position="333"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="region of interest" description="Kae1" evidence="16">
        <location>
            <begin position="1"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="region of interest" description="Threonylcarbamoyl-AMP binding" evidence="16">
        <location>
            <begin position="127"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor; for kinase activity" evidence="16">
        <location>
            <position position="452"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="106"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="110"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="127"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="159"/>
        </location>
        <ligand>
            <name>L-threonylcarbamoyladenylate</name>
            <dbReference type="ChEBI" id="CHEBI:73682"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="172"/>
        </location>
        <ligand>
            <name>L-threonylcarbamoyladenylate</name>
            <dbReference type="ChEBI" id="CHEBI:73682"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="176"/>
        </location>
        <ligand>
            <name>L-threonylcarbamoyladenylate</name>
            <dbReference type="ChEBI" id="CHEBI:73682"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="256"/>
        </location>
        <ligand>
            <name>L-threonylcarbamoyladenylate</name>
            <dbReference type="ChEBI" id="CHEBI:73682"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="284"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <begin position="339"/>
            <end position="347"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="360"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="AEF96187.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="536" mass="60541" checksum="7AB8465D81647201" modified="2011-07-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-06-02" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCH8</accession>
    <name>F6BCH8_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized conserved protein UCP921964</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_0639</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96189.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798794.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96189">
        <property type="protein sequence ID" value="AEF96189"/>
        <property type="gene ID" value="Metig_0639"/>
    </dbReference>
    <dbReference type="GeneID" id="10643478"/>
    <dbReference type="KEGG" id="mig:Metig_0639"/>
    <dbReference type="HOGENOM" id="CLU_143947_0_0_2"/>
    <dbReference type="OMA" id="IGCDCDY"/>
    <dbReference type="OrthoDB" id="86485at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014515">
        <property type="entry name" value="UCP921964"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09893">
        <property type="entry name" value="DUF2120"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF021964">
        <property type="entry name" value="UCP921964"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF96189.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="138" mass="15687" checksum="ED45A2C85AAB12A8" modified="2011-07-27" version="1">MHLKMTVGKIMQSLNAFKGSKPICDIEGLLIVRGVCRDENFEKYNSIKEYLEAKLKENGFEIADEDDIVLFVERINNVLKETMASPDMFGFEALKKSFESIGCECDYVIGKKGNLMVGVSMWFDKNRKNPKFVEVVGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-09-29" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCI9</accession>
    <name>F6BCI9_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Radical SAM domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Metig_0650</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96200.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798803.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96200">
        <property type="protein sequence ID" value="AEF96200"/>
        <property type="gene ID" value="Metig_0650"/>
    </dbReference>
    <dbReference type="GeneID" id="10643490"/>
    <dbReference type="KEGG" id="mig:Metig_0650"/>
    <dbReference type="HOGENOM" id="CLU_508670_0_0_2"/>
    <dbReference type="OMA" id="GLRPYVY"/>
    <dbReference type="OrthoDB" id="9274at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.30.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaB/NifB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023404">
        <property type="entry name" value="rSAM_horseshoe"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002792">
        <property type="entry name" value="TRAM_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01938">
        <property type="entry name" value="TRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50926">
        <property type="entry name" value="TRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="7">
        <location>
            <begin position="195"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="domain" description="TRAM" evidence="6">
        <location>
            <begin position="439"/>
            <end position="503"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEF96200.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="504" mass="57340" checksum="A2944BEE50FA5EE6" modified="2011-07-27" version="1">MKALIIDCLATVDGKKLLTRDVIGAGPRTVKGILKSEGIDAKIVPLEYFKVDDVKDYDLFFISGMTVDFKSVKNLVDKINENRDENQKIIIGGPIANDLYLLEKIEGDISIVGEGEITIRELIKKDFNAENVEGTTYYDYDKEELKINPLREIVKNMKLITPSTEIEDYPNYFSARVYVEVVRGCSNFKRALLLCDNKICNLCENGTIRCPLNINPGCGFCSVPSTFGYARSRDLEDILSEIEDLFKRGVKRVVLSAPDFLDYQREDDILINPKEPHPNYEAIEELLSNIYDLKIKYNANVSIENVKANLFDEKVAKIISKYLPNTPIYIGCETGDEMHSKLLGRPSLPEDVLKAVKIAKKYNLRPQVYFIYGLPGQNLESAINTINFMEKIKNYIDKITVYKFKPLPMSAFENSKPTMDKSSIMIKEKAREINKYIKKRYLGKKVEVIISERHFKNKKDAVGYMVSGGPMVVVKNGAEYIGKTVKVKIIKTYEKFVEGIILKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCJ3</accession>
    <name>F6BCJ3_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 5">50S ribosomal protein L21e</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">rpl21e</name>
        <name evidence="7" type="ordered locus">Metig_0654</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the eukaryotic ribosomal protein eL21 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96204.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798807.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0654"/>
    <dbReference type="EnsemblBacteria" id="AEF96204">
        <property type="protein sequence ID" value="AEF96204"/>
        <property type="gene ID" value="Metig_0654"/>
    </dbReference>
    <dbReference type="GeneID" id="10643494"/>
    <dbReference type="KEGG" id="mig:Metig_0654"/>
    <dbReference type="HOGENOM" id="CLU_103610_1_1_2"/>
    <dbReference type="OMA" id="KGMPHRR"/>
    <dbReference type="OrthoDB" id="107212at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00369">
        <property type="entry name" value="Ribosomal_L21e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036948">
        <property type="entry name" value="Ribosomal_L21_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001147">
        <property type="entry name" value="Ribosomal_L21e"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022856">
        <property type="entry name" value="Ribosomal_L21e_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018259">
        <property type="entry name" value="Ribosomal_L21e_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20981">
        <property type="entry name" value="PTHR20981"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01157">
        <property type="entry name" value="Ribosomal_L21e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="SSF50104"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01171">
        <property type="entry name" value="RIBOSOMAL_L21E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 5 7" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00029517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEF96204.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="98" mass="11359" checksum="DA340FEEB6C441D0" modified="2011-07-27" version="1">MAQRSEGFRSKTRKKLSKHPRERGLYPITRALKQYKEGDYVHIVIDPSYHKGMPHPRFHGRTGIVVGQRGRAFIVKVRDGGKYKLIIARPQHLRPCKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCN2</accession>
    <name>F6BCN2_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="4">THUMP domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metig_0693</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96243.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798846.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0693"/>
    <dbReference type="EnsemblBacteria" id="AEF96243">
        <property type="protein sequence ID" value="AEF96243"/>
        <property type="gene ID" value="Metig_0693"/>
    </dbReference>
    <dbReference type="GeneID" id="10643533"/>
    <dbReference type="KEGG" id="mig:Metig_0693"/>
    <dbReference type="HOGENOM" id="CLU_795999_0_0_2"/>
    <dbReference type="OMA" id="ESRYQIQ"/>
    <dbReference type="OrthoDB" id="27531at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
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        <property type="entry name" value="MJ0421-like_THUMP"/>
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        <location>
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            <end position="148"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
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        <source>
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    <sequence length="363" mass="41635" checksum="EAB0DD26D638805B" modified="2011-07-27" version="1">MKFIIKTQKGYEGIAANYLKEEVEDIKVHITPHGYSGIVIVESEDDITDKALKIPEIERVLKVLFEVPADLEKIVSLADKIAEHINEGESFAVETKRRGKHNFSSVDVNIVLGAKIKDLTNAEVNLDYPDKVVYVEIIKDIAYICIGERKEWRKYESNKKNARELFKKVTIVQMPYWGELKVCRKFGEQIGRAAQAFEVKELIIAPKGKIDAYELLEFLKGVQIGQKSRYLVQKEAYPWEVKKVPVSVHELYQVVRDKRRNNRLLIVTDPKGNTLNQVKDKLKKDLKFAKEVIIFIGSREGIPRGIFRFADYVIDLAPYMTFATEHGIPGALIGLWTIYDEENDDNENNDENNENSGNEETTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2020-06-17" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCR7</accession>
    <name>F6BCR7_METIK</name>
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        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
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            <taxon>Methanococci</taxon>
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        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
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                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
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            <dbReference type="EMBL" id="AEF96278.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="205" mass="23680" checksum="FA6287D4DE570DAA" modified="2011-07-27" version="1">MMLVSEESKDNFIAFVITGNGRWEQVILKSIAKRYNGRDKVLYFPKSPFKHSRKTGLSCLDILRLEKEKLNIIKKCGKLLVLIDKEHISSVDDITKKLESLRVFEDFEMENNGDIVKIIGNLGNREIVIHIVIFGKEKCIEEDVSELIKLEFGENIYPDKNAIKDFLKSKGLKRKDLIEDAKIENLEIAFNPLIRVIKFLEGEKL</sequence>
</entry>
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    <name>THI4_METIK</name>
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        <recommendedName>
            <fullName evidence="3">Thiamine thiazole synthase</fullName>
            <ecNumber evidence="2">2.4.2.59</ecNumber>
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    </protein>
    <gene>
        <name evidence="3" type="primary">thi4</name>
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            <taxon>Methanococci</taxon>
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            <taxon>Methanotorris</taxon>
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    </organism>
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        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
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                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2016" name="Biochemistry" volume="55" first="1826" last="1838">
            <title>Structural basis for iron-mediated sulfur transfer in archael and yeast thiazole synthases.</title>
            <authorList>
                <person name="Zhang X."/>
                <person name="Eser B.E."/>
                <person name="Chanani P.K."/>
                <person name="Begley T.P."/>
                <person name="Ealick S.E."/>
            </authorList>
            <dbReference type="PubMed" id="26919468"/>
            <dbReference type="DOI" id="10.1021/acs.biochem.6b00030"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.10 ANGSTROMS) IN COMPLEX WITH IRON AND A GLYCINE IMINE INTERMEDIATE</scope>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>COFACTOR</scope>
        <scope>SUBUNIT</scope>
        <scope>PATHWAY</scope>
    </reference>
    <comment type="function">
        <text evidence="1 4">Involved in the biosynthesis of the thiazole moiety of thiamine. Catalyzes the conversion of NAD and glycine to adenosine diphosphate 5-(2-hydroxyethyl)-4-methylthiazole-2-carboxylate (ADT), an adenylated thiazole intermediate, using free sulfide as a source of sulfur.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>glycine + hydrogen sulfide + NAD(+) = ADP-5-ethyl-4-methylthiazole-2-carboxylate + H(+) + 3 H2O + nicotinamide</text>
            <dbReference type="Rhea" id="RHEA:55704"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17154"/>
            <dbReference type="ChEBI" id="CHEBI:29919"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:139151"/>
            <dbReference type="EC" id="2.4.2.59"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
        <text evidence="2">Binds 1 Fe(2+) ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4">Cofactor biosynthesis; thiamine diphosphate biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homooctamer; tetramer of dimers.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the THI4 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.59" evidence="2"/>
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        <property type="protein sequence ID" value="AEF96285.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="PDB" id="4Y4N">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.10 A"/>
        <property type="chains" value="A/B/C/D/E/F/G/H=1-263"/>
    </dbReference>
    <dbReference type="PDBsum" id="4Y4N"/>
    <dbReference type="AlphaFoldDB" id="F6BCS4"/>
    <dbReference type="SMR" id="F6BCS4"/>
    <dbReference type="STRING" id="880724.Metig_0735"/>
    <dbReference type="EnsemblBacteria" id="AEF96285">
        <property type="protein sequence ID" value="AEF96285"/>
        <property type="gene ID" value="Metig_0735"/>
    </dbReference>
    <dbReference type="GeneID" id="10643575"/>
    <dbReference type="KEGG" id="mig:Metig_0735"/>
    <dbReference type="HOGENOM" id="CLU_053727_2_0_2"/>
    <dbReference type="OMA" id="MWGGGMM"/>
    <dbReference type="OrthoDB" id="61905at2157"/>
    <dbReference type="BRENDA" id="2.4.2.59">
        <property type="organism ID" value="13534"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00060"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016763">
        <property type="term" value="F:pentosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009228">
        <property type="term" value="P:thiamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009229">
        <property type="term" value="P:thiamine diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00304">
        <property type="entry name" value="Thi4"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002922">
        <property type="entry name" value="Thi4_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022828">
        <property type="entry name" value="Thi4_prok"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00292">
        <property type="entry name" value="TIGR00292"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0520">NAD</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0784">Thiamine biosynthesis</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000438857" description="Thiamine thiazole synthase">
        <location>
            <begin position="1"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="binding site" description="in other chain" evidence="4">
        <location>
            <position position="40"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <note>ligand shared between two adjacent protomers</note>
        </ligand>
    </feature>
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            <begin position="59"/>
            <end position="60"/>
        </location>
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            <name>NAD(+)</name>
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            <note>ligand shared between two adjacent protomers</note>
        </ligand>
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            <position position="67"/>
        </location>
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            <name>NAD(+)</name>
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        </ligand>
    </feature>
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            <position position="133"/>
        </location>
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            <name>NAD(+)</name>
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        </ligand>
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    <feature type="binding site" evidence="1">
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        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
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        </ligand>
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        <location>
            <position position="161"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
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        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="2">
        <location>
            <position position="176"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
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        </ligand>
    </feature>
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        </location>
        <ligand>
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        </location>
        <ligand>
            <name>NAD(+)</name>
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        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="236"/>
        </location>
        <ligand>
            <name>glycine</name>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
        </ligand>
    </feature>
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        </location>
    </feature>
    <feature type="helix" evidence="7">
        <location>
            <begin position="226"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="helix" evidence="7">
        <location>
            <begin position="242"/>
            <end position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="26919468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="3">
        <source>
            <dbReference type="PubMed" id="26919468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4">
        <source>
            <dbReference type="PubMed" id="26919468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000312" key="5">
        <source>
            <dbReference type="EMBL" id="AEF96285.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="7">
        <source>
            <dbReference type="PDB" id="4Y4N"/>
        </source>
    </evidence>
    <sequence length="263" mass="28128" checksum="282EFB2FC7035ED1" modified="2011-07-27" version="1">MDVRLRADEYATTRAILKSAFDMWLDIIDVDVAIVGGGPSGLTAARYIAKEGYKVVVLERHLAFGGGTWGGGMGFPYIVVEEPADEILREVGVKLEKVEGEDGLYTADSVEVPAKLAVGAIDAGAKVLTGIVVEDLVLRENRVAGVVINSYAIEKAGLHIDPITITAKYVVDATGHDASVVTTLSRKNPELGLEVPGEKSMWAEKGENALLRNTREVYPGLFVCGMAANAVYAGHRMGAIFGGMYISGKKCAEMIVEKLKNNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCW1</accession>
    <name>F6BCW1_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Phosphopantetheine adenylyltransferase</fullName>
            <ecNumber evidence="2">2.7.7.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">Dephospho-CoA pyrophosphorylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="2">Pantetheine-phosphate adenylyltransferase</fullName>
            <shortName evidence="2">PPAT</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2" type="primary">coaD</name>
        <name evidence="4" type="ordered locus">Metig_0776</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Reversibly transfers an adenylyl group from ATP to 4'-phosphopantetheine, yielding dephospho-CoA (dPCoA) and pyrophosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>(R)-4'-phosphopantetheine + ATP + H(+) = 3'-dephospho-CoA + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:19801"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57328"/>
            <dbReference type="ChEBI" id="CHEBI:61723"/>
            <dbReference type="EC" id="2.7.7.3"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Cofactor biosynthesis; coenzyme A biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eukaryotic CoaD family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.3" evidence="2"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96322.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798925.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0776"/>
    <dbReference type="EnsemblBacteria" id="AEF96322">
        <property type="protein sequence ID" value="AEF96322"/>
        <property type="gene ID" value="Metig_0776"/>
    </dbReference>
    <dbReference type="GeneID" id="10643616"/>
    <dbReference type="KEGG" id="mig:Metig_0776"/>
    <dbReference type="HOGENOM" id="CLU_035272_5_0_2"/>
    <dbReference type="OMA" id="FDTLHSG"/>
    <dbReference type="OrthoDB" id="93412at2157"/>
    <dbReference type="UniPathway" id="UPA00241"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004595">
        <property type="term" value="F:pantetheine-phosphate adenylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015937">
        <property type="term" value="P:coenzyme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00647">
        <property type="entry name" value="PPAT_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004821">
        <property type="entry name" value="Cyt_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023540">
        <property type="entry name" value="PPAT_arch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01467">
        <property type="entry name" value="CTP_transf_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00125">
        <property type="entry name" value="cyt_tran_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0173">Coenzyme A biosynthesis</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2 4" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="CTP_transf_like" evidence="3">
        <location>
            <begin position="6"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEF96322.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="150" mass="17270" checksum="6791B7F96B1B8EEC" modified="2011-07-27" version="1">MPNKVVVGGTFDILHEGHKKLIIFASKLGKLFIGITSDEFAKKYKKHEINPLNIRIENLKKFLEENNIDYELKVIDNPYGDATEEDYDAIVVSPETKKTAEKINEIRIKKGLKPLKIYVYDYVLAEDGKPISTTRIREKIIDEKGHLLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCY4</accession>
    <name>F6BCY4_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Cation transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Metig_0800</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96345.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798948.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0800"/>
    <dbReference type="EnsemblBacteria" id="AEF96345">
        <property type="protein sequence ID" value="AEF96345"/>
        <property type="gene ID" value="Metig_0800"/>
    </dbReference>
    <dbReference type="GeneID" id="10643641"/>
    <dbReference type="KEGG" id="mig:Metig_0800"/>
    <dbReference type="HOGENOM" id="CLU_030708_3_0_2"/>
    <dbReference type="OMA" id="LQWMGGM"/>
    <dbReference type="OrthoDB" id="13996at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008324">
        <property type="term" value="F:cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006813">
        <property type="term" value="P:potassium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003445">
        <property type="entry name" value="Cat_transpt"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02386">
        <property type="entry name" value="TrkH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="43"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="73"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="138"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="184"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="232"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="273"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="316"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="383"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="442"/>
            <end position="463"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEF96345.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="470" mass="52229" checksum="F108EC76BADA5E33" modified="2011-07-27" version="1">MKLFKREDIIGIVHELGYLMMIVGFLMIVPIFVGIYYNENYENFIFSAAFSIFVGALLRGLTKPIEIKLKHAMVISAVTWLMASLLGALPFYFGIDYFSYVDAVFESMSAWTTTGFSLINNVEAMPHTILFWRSLEQWIGGVGVLVMMIGILMRSGTAAALFYKAEAREEKILPSTVNTVKTIWWIYLLYTILGICGLYLSGLSLWEAVNLTMCGICTGGMSVNNLSFPYNTMAKVIMILIMVIGGVISFSIHHKILTGKSFRDIQTDFAVKIFIVASIIVAISSNTDIIDALFTVVSAMTSTGYTTIDIKGLDNLSIAILIFLMAIGGGAGTTTGGIKIIRFLVMVKTFYYELKEIFFPPSAVIHEKLGNVVLDYKVIRESFVIGFVFVMHYIFTAMVFIALGYDPYKSIFEAVSLTSNIGMSMGIVNINMPLIGKIVGILAMWIGRLEILPIYVFVLLPIVNKIKKLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCY6</accession>
    <name>F6BCY6_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Transcription factor S</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Transcription elongation factor IIS/RNA polymerase subunit homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">Metig_0802</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="7 9">Belongs to the archaeal rpoM/eukaryotic RPA12/RPB9/RPC11 RNA polymerase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96347.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798950.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0802"/>
    <dbReference type="EnsemblBacteria" id="AEF96347">
        <property type="protein sequence ID" value="AEF96347"/>
        <property type="gene ID" value="Metig_0802"/>
    </dbReference>
    <dbReference type="GeneID" id="10643643"/>
    <dbReference type="KEGG" id="mig:Metig_0802"/>
    <dbReference type="HOGENOM" id="CLU_093932_3_2_2"/>
    <dbReference type="OMA" id="MVKFCPK"/>
    <dbReference type="OrthoDB" id="121393at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019761">
        <property type="entry name" value="DNA-dir_RNA_pol-M_15_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001529">
        <property type="entry name" value="DNA-dir_RNA_pol_M/15kDasu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012164">
        <property type="entry name" value="Rpa12/Rpb9/Rpc10/TFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006288">
        <property type="entry name" value="TFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001222">
        <property type="entry name" value="Znf_TFIIS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11239">
        <property type="entry name" value="PTHR11239"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02150">
        <property type="entry name" value="RNA_POL_M_15KD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01096">
        <property type="entry name" value="TFIIS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005586">
        <property type="entry name" value="RNApol_RpoM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00661">
        <property type="entry name" value="RPOL9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00440">
        <property type="entry name" value="ZnF_C2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01384">
        <property type="entry name" value="TFS_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01030">
        <property type="entry name" value="RNA_POL_M_15KD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00466">
        <property type="entry name" value="ZF_TFIIS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51133">
        <property type="entry name" value="ZF_TFIIS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7 9" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="TFIIS-type" evidence="10">
        <location>
            <begin position="62"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00018272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00032962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR005586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AEF96347.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="105" mass="12474" checksum="F5270A258E002318" modified="2011-07-27" version="1">MIEFCPKCKNIMLPKGGMLKCTVCGYEMELKEENTTYEFKEKIEHKQPEVTVIEQVDTLPTTRVECPKCGHMEASWWLQQTRCADEPETRFYKCKKCGHTWREYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCZ9</accession>
    <name>F6BCZ9_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="3">High-affinity nickel-transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_0815</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96360.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798962.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0815"/>
    <dbReference type="EnsemblBacteria" id="AEF96360">
        <property type="protein sequence ID" value="AEF96360"/>
        <property type="gene ID" value="Metig_0815"/>
    </dbReference>
    <dbReference type="GeneID" id="10643656"/>
    <dbReference type="KEGG" id="mig:Metig_0815"/>
    <dbReference type="HOGENOM" id="CLU_058605_4_1_2"/>
    <dbReference type="OMA" id="HALEPGH"/>
    <dbReference type="OrthoDB" id="93540at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039447">
        <property type="entry name" value="UreH-like_TM_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13386">
        <property type="entry name" value="DsbD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="41"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="78"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="111"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="143"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="186"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="domain" description="DsbD_2" evidence="2">
        <location>
            <begin position="50"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13386"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF96360.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="215" mass="22828" checksum="3861E623286B5392" modified="2011-07-27" version="1">MELVFTATAFILGMLHALEPGHGKSVLAAYVLGTKANIKDAITLGVTITLSHIFVVFMLGILSIYLIKSVSASIAHDLMGVIGGAILIGVGLWILNGYFHPHEHKIDTKKGIIAIGLSAGLTPCPAALAVLLFSIVEGDVLNGLIYVVVFSIGLALSLTTLSVLFIKSKEFIQKYAGSEKINKLPLISGTIIILIGLYIVVLQITALSIISNFKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>F6BD01</accession>
    <name>F6BD01_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Cobalt transport protein CbiN</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Energy-coupling factor transporter probable substrate-capture protein CbiN</fullName>
            <shortName evidence="10">ECF transporter S component CbiN</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">cbiN</name>
        <name evidence="11" type="ordered locus">Metig_0817</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Part of the energy-coupling factor (ECF) transporter complex CbiMNOQ involved in cobalt import.</text>
    </comment>
    <comment type="pathway">
        <text evidence="10">Cofactor biosynthesis; adenosylcobalamin biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Forms an energy-coupling factor (ECF) transporter complex composed of an ATP-binding protein (A component, CbiO), a transmembrane protein (T component, CbiQ) and 2 possible substrate-capture proteins (S components, CbiM and CbiN) of unknown stoichimetry.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cell membrane</location>
            <topology evidence="10">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the CbiN family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96362.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798964.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0817"/>
    <dbReference type="EnsemblBacteria" id="AEF96362">
        <property type="protein sequence ID" value="AEF96362"/>
        <property type="gene ID" value="Metig_0817"/>
    </dbReference>
    <dbReference type="GeneID" id="10643658"/>
    <dbReference type="KEGG" id="mig:Metig_0817"/>
    <dbReference type="HOGENOM" id="CLU_136197_2_0_2"/>
    <dbReference type="OMA" id="TEIEPDY"/>
    <dbReference type="UniPathway" id="UPA00148"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015087">
        <property type="term" value="F:cobalt ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00330">
        <property type="entry name" value="CbiN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003705">
        <property type="entry name" value="CbiN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38662">
        <property type="entry name" value="PTHR38662"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02553">
        <property type="entry name" value="CbiN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01165">
        <property type="entry name" value="cbiN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 10" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 10" id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword evidence="9 10" id="KW-0170">Cobalt</keyword>
    <keyword evidence="1 10" id="KW-0171">Cobalt transport</keyword>
    <keyword evidence="7 10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 10" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="7"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="69"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022426"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AEF96362.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="99" mass="10911" checksum="578B149B5368E59C" modified="2011-07-27" version="1">METKHILMLLGVVILTVVPLIMYAGHGEDDGYFGGADDAAEEAITQLDPNYEPWFKPIWEPPSGEIESLLFALQAAIGAIIIGYFIGYNKAKRELVSQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2020-08-12" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>F6BD26</accession>
    <name>F6BD26_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0843</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96387.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96387">
        <property type="protein sequence ID" value="AEF96387"/>
        <property type="gene ID" value="Metig_0843"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0843"/>
    <dbReference type="HOGENOM" id="CLU_1131620_0_0_2"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="29"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="90"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="134"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="160"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96387.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="232" mass="26467" checksum="1D4B2B627CDED8EB" modified="2011-07-27" version="1">MKGEILGFGIRIEFIENLFERFLELNTKLKFIVTYVMCWVGFLFSLSIGKLISNILYEPVEVVSEVGTAKFHVVSSAVSSKMGVSYYSIVLSYFINNASACLIIVSAFVLVSHLYKKEISKDLSNFKEYLRTLLLFYLIVVINPITGFVGCFIDSNKRKVCVFSSSVIFIYHEILIIYDSTVSDMWGLKPEWRLLRALKMWGVSVPPESQPMKMGGWKVIRYDSFVCFKSSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2020-06-17" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>F6BD77</accession>
    <name>F6BD77_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_0895</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96438.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96438">
        <property type="protein sequence ID" value="AEF96438"/>
        <property type="gene ID" value="Metig_0895"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0895"/>
    <dbReference type="HOGENOM" id="CLU_2748194_0_0_2"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF96438.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="70" mass="8341" checksum="FDCB045F4EA7C1BE" modified="2011-07-27" version="1">MGLFSIRYKPKIEQDKNSAINTTKKSIHKNDLIFFKISFSPNLVMLFCNPSQSKIRKIRIRIIKLYFTYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>F6BD86</accession>
    <name>F6BD86_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">30S ribosomal protein S14 type Z</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">rps14</name>
        <name evidence="4" type="ordered locus">Metig_0904</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Binds 16S rRNA, required for the assembly of 30S particles.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="3">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Part of the 30S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the universal ribosomal protein uS14 family. Zinc-binding uS14 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96447.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799049.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0904"/>
    <dbReference type="EnsemblBacteria" id="AEF96447">
        <property type="protein sequence ID" value="AEF96447"/>
        <property type="gene ID" value="Metig_0904"/>
    </dbReference>
    <dbReference type="GeneID" id="10643748"/>
    <dbReference type="KEGG" id="mig:Metig_0904"/>
    <dbReference type="HOGENOM" id="CLU_177289_2_2_2"/>
    <dbReference type="OMA" id="HCFREIA"/>
    <dbReference type="OrthoDB" id="122051at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.830.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01364_A">
        <property type="entry name" value="Ribosomal_S14_2_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039744">
        <property type="entry name" value="40S_S29/30S_S14z"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001209">
        <property type="entry name" value="Ribosomal_S14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043140">
        <property type="entry name" value="Ribosomal_S14/S29"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018271">
        <property type="entry name" value="Ribosomal_S14_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023676">
        <property type="entry name" value="Ribosomal_S14_type-Z_arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12010">
        <property type="entry name" value="PTHR12010"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00253">
        <property type="entry name" value="Ribosomal_S14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00527">
        <property type="entry name" value="RIBOSOMAL_S14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="1 3 4" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3" id="KW-0699">rRNA-binding</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="binding site" evidence="3">
        <location>
            <position position="17"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="20"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="36"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="39"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEF96447.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="53" mass="6111" checksum="14C5A06F3E0AF0D3" modified="2011-07-27" version="1">MAKAPWKRKYGIGIRPCRRCGHVGPGLIRKYGLNLCRQCFREVAAKLGFKKYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>F6BD91</accession>
    <name>F6BD91_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">30S ribosomal protein S17</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">rps17</name>
        <name evidence="4" type="ordered locus">Metig_0909</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">One of the primary rRNA binding proteins, it binds specifically to the 5'-end of 16S ribosomal RNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Part of the 30S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 2 3">Belongs to the universal ribosomal protein uS17 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96452.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799054.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0909"/>
    <dbReference type="EnsemblBacteria" id="AEF96452">
        <property type="protein sequence ID" value="AEF96452"/>
        <property type="gene ID" value="Metig_0909"/>
    </dbReference>
    <dbReference type="GeneID" id="10643753"/>
    <dbReference type="KEGG" id="mig:Metig_0909"/>
    <dbReference type="HOGENOM" id="CLU_073626_0_3_2"/>
    <dbReference type="OMA" id="KKYERYE"/>
    <dbReference type="OrthoDB" id="108145at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01345_A">
        <property type="entry name" value="Ribosomal_S17_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000266">
        <property type="entry name" value="Ribosomal_S17/S11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028333">
        <property type="entry name" value="Ribosomal_S17_arc-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019978">
        <property type="entry name" value="Ribosomal_S17_archaeal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019979">
        <property type="entry name" value="Ribosomal_S17_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10744">
        <property type="entry name" value="PTHR10744"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00366">
        <property type="entry name" value="Ribosomal_S17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00973">
        <property type="entry name" value="RIBOSOMALS17"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03630">
        <property type="entry name" value="uS17_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00056">
        <property type="entry name" value="RIBOSOMAL_S17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 3 4" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="2" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2" id="KW-0699">rRNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEF96452.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="115" mass="13107" checksum="5AA4BDBAB28B73BC" modified="2011-07-27" version="1">MRNIGIPVNAPKKECNDKNCPFHGTLSVRGQMFEGVVVSDKAHKTVVIKREIVKYLKKYERYEKRTSKLVAHNPECINAKVGDIVRVMECRPISKTKSFVVIEKLGHVKEVKGEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>F6BD96</accession>
    <name>F6BD96_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 6">50S ribosomal protein L22</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">rpl22</name>
        <name evidence="7" type="ordered locus">Metig_0914</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">The globular domain of the protein is located near the polypeptide exit tunnel on the outside of the subunit, while an extended beta-hairpin is found that lines the wall of the exit tunnel in the center of the 70S ribosome.</text>
    </comment>
    <comment type="function">
        <text evidence="4 6">This protein binds specifically to 23S rRNA. It makes multiple contacts with different domains of the 23S rRNA in the assembled 50S subunit and ribosome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4 6">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4 5">Belongs to the universal ribosomal protein uL22 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96457.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0914"/>
    <dbReference type="EnsemblBacteria" id="AEF96457">
        <property type="protein sequence ID" value="AEF96457"/>
        <property type="gene ID" value="Metig_0914"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0914"/>
    <dbReference type="HOGENOM" id="CLU_083987_0_2_2"/>
    <dbReference type="OMA" id="ANAEYKG"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015934">
        <property type="term" value="C:large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00336">
        <property type="entry name" value="Ribosomal_L22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.470.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01331_A">
        <property type="entry name" value="Ribosomal_L22_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001063">
        <property type="entry name" value="Ribosomal_L22"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018260">
        <property type="entry name" value="Ribosomal_L22/L17_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005721">
        <property type="entry name" value="Ribosomal_L22/L17_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036394">
        <property type="entry name" value="Ribosomal_L22/L17_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11593">
        <property type="entry name" value="PTHR11593"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00237">
        <property type="entry name" value="Ribosomal_L22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54843">
        <property type="entry name" value="SSF54843"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01038">
        <property type="entry name" value="uL22_arch_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00464">
        <property type="entry name" value="RIBOSOMAL_L22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 5" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="4 6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4 6" id="KW-0699">rRNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU004005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEF96457.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="177" mass="20630" checksum="F467A3384E3F4359" modified="2011-07-27" version="1">MMIMYGIKKHVDITSLNWDFEVNGMAKLNYKIETDPKKTARAMGRALKISRKHAVEICRELNGMKLDDAIQYLKDVIALKRFVPFKRHCKDVPHRKGKFGWHAGRYPVKASREILKVLENAKKNAEYKGLNVEKLRIKHISSNKGPTIKRYMPRAFGRASPKFQETVHIQVILEEYH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDA2</accession>
    <name>F6BDA2_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_0920</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96463.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799065.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96463">
        <property type="protein sequence ID" value="AEF96463"/>
        <property type="gene ID" value="Metig_0920"/>
    </dbReference>
    <dbReference type="GeneID" id="10643764"/>
    <dbReference type="KEGG" id="mig:Metig_0920"/>
    <dbReference type="HOGENOM" id="CLU_091350_1_0_2"/>
    <dbReference type="OMA" id="GLCIGHD"/>
    <dbReference type="OrthoDB" id="78172at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014997">
        <property type="entry name" value="DUF1847"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08901">
        <property type="entry name" value="DUF1847"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF96463.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="191" mass="22191" checksum="61ECA7C8CF6C1BC9" modified="2011-07-27" version="1">MRCARCNRKDCQNGKNCKENILKEILKEYKKEENLKIAKVASFIEGNFYMKKTRLEEIIEFCKMMNYKRIGIAFCIGLEKEAEILDKILSKHFEVYSVCCKVCGINKDEFELTHINKNQKETMCNPIAQALILNEKKTDLNIILGLCVGHDILFQKYSNAPTTTFAVKDRVLTHNPLGVIYSKYYLRKFGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2020-06-17" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDC8</accession>
    <name>F6BDC8_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0946</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96489.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96489">
        <property type="protein sequence ID" value="AEF96489"/>
        <property type="gene ID" value="Metig_0946"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0946"/>
    <dbReference type="HOGENOM" id="CLU_1922805_0_0_2"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="39"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="71"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96489.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="131" mass="15362" checksum="57024AA17A0D006A" modified="2011-07-27" version="1">MCIIGKMLLVVCIGFVKYTMMFFGVGFLLSLIILYVDIYVVRIFLGIILALLWFVYIWYVAYKILVSKYCWIILLPVLLLALMSGIIPFILLVLWIYVCRVITISSKRSLEKKGIYNLFDLIRYIRKSSKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDJ7</accession>
    <name>F6BDJ7_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Digeranylgeranylglyceryl phosphate synthase</fullName>
            <shortName evidence="10">DGGGP synthase</shortName>
            <shortName evidence="10">DGGGPS</shortName>
            <ecNumber evidence="10">2.5.1.42</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">(S)-2,3-di-O-geranylgeranylglyceryl phosphate synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Geranylgeranylglycerol-phosphate geranylgeranyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">Metig_1016</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Prenyltransferase that catalyzes the transfer of the geranylgeranyl moiety of geranylgeranyl diphosphate (GGPP) to the C2 hydroxyl of (S)-3-O-geranylgeranylglyceryl phosphate (GGGP). This reaction is the second ether-bond-formation step in the biosynthesis of archaeal membrane lipids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>(2E,6E,10E)-geranylgeranyl diphosphate + sn-3-O-(geranylgeranyl)glycerol 1-phosphate = 2,3-bis-O-(geranylgeranyl)-sn-glycerol 1-phosphate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:18109"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57677"/>
            <dbReference type="ChEBI" id="CHEBI:58756"/>
            <dbReference type="ChEBI" id="CHEBI:58837"/>
            <dbReference type="EC" id="2.5.1.42"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="10">Membrane lipid metabolism; glycerophospholipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Cell membrane</location>
            <topology evidence="1 10">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the UbiA prenyltransferase family. DGGGP synthase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.42" evidence="10"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96558.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799160.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1016"/>
    <dbReference type="EnsemblBacteria" id="AEF96558">
        <property type="protein sequence ID" value="AEF96558"/>
        <property type="gene ID" value="Metig_1016"/>
    </dbReference>
    <dbReference type="GeneID" id="10643870"/>
    <dbReference type="KEGG" id="mig:Metig_1016"/>
    <dbReference type="HOGENOM" id="CLU_073311_1_1_2"/>
    <dbReference type="OMA" id="DYFDYEI"/>
    <dbReference type="OrthoDB" id="104789at2157"/>
    <dbReference type="UniPathway" id="UPA00940"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047295">
        <property type="term" value="F:geranylgeranylglycerol-phosphate geranylgeranyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006650">
        <property type="term" value="P:glycerophospholipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01286">
        <property type="entry name" value="DGGGP_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023547">
        <property type="entry name" value="DGGGP_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000537">
        <property type="entry name" value="UbiA_prenyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044878">
        <property type="entry name" value="UbiA_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01040">
        <property type="entry name" value="UbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="2 10" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="6 10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="4 10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 10" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="9 10" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="33"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="87"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="128"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="198"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="224"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="256"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AEF96558.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="276" mass="31292" checksum="F263B48CA9AFC0AA" modified="2011-07-27" version="1">MMKYYLELMRVKNCITASFGAFIGGLIASNFNFGYLFVLILAFLVVFFICGFGNVVNDIYDVEIDKLNKPHRPLPSNKISIKNAWRFAWLLLIFGLVLSFFNVLCFIIALINSVMLYLYAKKYKRNKIIGNFIVAYLTGSVFLFGGVAVNNMPIVTILFLCAMFATWCREIIKDFEDIDGDMKEGVVSLPIKYGKKSLYIAAMFIIVAVALSPLPYIMGIFGEIYLILIILCDLSFLYVIFKAIKNPSKNIMSKTSKYLKIIMNLVLMCFVLGALM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-06-02" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDQ1</accession>
    <name>F6BDQ1_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1073</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96612.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799213.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96612">
        <property type="protein sequence ID" value="AEF96612"/>
        <property type="gene ID" value="Metig_1073"/>
    </dbReference>
    <dbReference type="GeneID" id="10643927"/>
    <dbReference type="KEGG" id="mig:Metig_1073"/>
    <dbReference type="HOGENOM" id="CLU_2243863_0_0_2"/>
    <dbReference type="OrthoDB" id="101247at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="49"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="82"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96612.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="104" mass="12008" checksum="4B4BE9ABAA44FB51" modified="2011-07-27" version="1">MKDLILNPDKFFKDILTKVSFVYLIEMTVALIIQLISILTNTLYADGGYIIKLMDIMVSLWAMLLFAKGIQYNYKLAFKKSLVIPFLIFLLNLLSSIADYINIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDQ2</accession>
    <name>F6BDQ2_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Phosphonate-transporting ATPase</fullName>
            <ecNumber evidence="5">3.6.3.28</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Metig_1074</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.6.3.28" evidence="5"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96613.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799214.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1074"/>
    <dbReference type="EnsemblBacteria" id="AEF96613">
        <property type="protein sequence ID" value="AEF96613"/>
        <property type="gene ID" value="Metig_1074"/>
    </dbReference>
    <dbReference type="GeneID" id="10643928"/>
    <dbReference type="KEGG" id="mig:Metig_1074"/>
    <dbReference type="HOGENOM" id="CLU_000604_1_22_2"/>
    <dbReference type="OMA" id="EFIYIIG"/>
    <dbReference type="OrthoDB" id="67630at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03255">
        <property type="entry name" value="ABC_MJ0796_LolCDE_FtsE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017911">
        <property type="entry name" value="MacB_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="2"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="38"/>
            <end position="45"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEF96613.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="225" mass="25129" checksum="F84CA44BA2A2D61D" modified="2011-07-27" version="1">MIELKNVTKTYKMGGEIIYALKNVNLKIKEGEFVVIMGPSGSGKSTLLNIIGCLDKPDSGEVYIDKIKINDLNDEELTKIRRNKIGFVFQQFNLIPLLTALENVELPLIFKYRNTLSEEERKKIALKCLKMAELDEKFANHKPNQLSGGQQQRVAIARALANNPPILLCDEPTGSLDSKTGGKIMELLKKLNDSGKTVVVVTHDLAVAKYADRVIYLRDGNIVEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDR8</accession>
    <name>F6BDR8_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="3">2-oxoglutarate synthase</fullName>
            <ecNumber evidence="3">1.2.7.3</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_1090</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.2.7.3" evidence="3"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96629.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1090"/>
    <dbReference type="EnsemblBacteria" id="AEF96629">
        <property type="protein sequence ID" value="AEF96629"/>
        <property type="gene ID" value="Metig_1090"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_1090"/>
    <dbReference type="HOGENOM" id="CLU_017038_0_1_2"/>
    <dbReference type="OMA" id="DFIQGDE"/>
    <dbReference type="OrthoDB" id="34838at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047553">
        <property type="term" value="F:2-oxoglutarate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006082">
        <property type="term" value="P:organic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901576">
        <property type="term" value="P:organic substance biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044272">
        <property type="term" value="P:sulfur compound biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="CDD" id="cd07034">
        <property type="entry name" value="TPP_PYR_PFOR_IOR-alpha_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033412">
        <property type="entry name" value="PFOR_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002880">
        <property type="entry name" value="Pyrv_Fd/Flavodoxin_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009014">
        <property type="entry name" value="Transketo_C/PFOR_II"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17147">
        <property type="entry name" value="PFOR_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01855">
        <property type="entry name" value="POR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="SSF52518"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52922">
        <property type="entry name" value="SSF52922"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="POR_N" evidence="1">
        <location>
            <begin position="9"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="domain" description="PFOR_II" evidence="2">
        <location>
            <begin position="263"/>
            <end position="353"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF17147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF96629.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="359" mass="39960" checksum="931B5EA0C00FCFEE" modified="2011-07-27" version="1">MQGNMACVEGAIKAGCRFFAGYPITPSTEIAEGMAKKLPKVGGYYVQMEDEIASIAAVIGASWGGLKSMTATSGPGFSLMQENIGYAFMTETPCVIVNIQRGGPSTGQPTMASQGDMMQARWGSHGDYEPIVISPSSVQEMYDFTIMAFNYAEKYRVPVLLMADEIVGHMREKVVLHDNFEIIDRKKPEEKPCKNPYPFDVEVPPMPVFGEGYNVHITGLTHNEKGYPDVSAETHDKLVRRIVNKIRKNKNDIVKYEGKYLDADVIFVCYGTPSRTVKYTVEELRKKGVDVGYVRLITVFPFPDELIKGLDASKVIVPEMNLGQIYYEVERCAKGDVVLVDKIGGELHRPEELEKAVFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDR9</accession>
    <name>F6BDR9_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_1091</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96630.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799231.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96630">
        <property type="protein sequence ID" value="AEF96630"/>
        <property type="gene ID" value="Metig_1091"/>
    </dbReference>
    <dbReference type="GeneID" id="10643946"/>
    <dbReference type="KEGG" id="mig:Metig_1091"/>
    <dbReference type="HOGENOM" id="CLU_1357933_0_0_2"/>
    <dbReference type="OMA" id="ISYCYYR"/>
    <dbReference type="OrthoDB" id="100359at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF96630.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="209" mass="24773" checksum="8E8103265AFAA236" modified="2011-07-27" version="1">MKKMLCFFSLLIVVSFCGCFEENKVADGINKTIVNNSKVIGVGNFTLNDTLNKTNQSEKKYEKEKNNTTINKTQNFDFSKIDTQNVPIDDIFLNLNYVNEIDASNDNGKRKIKIYKINDITVVFDVSEYRINFYDAMEYNKVKKYGLRDKFGSYICYEFTPYNQNGTKSYCYVRKIGKYWIIMSFNKKDKKVNELWKEWNEYIISRFKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDU0</accession>
    <name>F6BDU0_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Thiamine thiazole synthase</fullName>
            <ecNumber evidence="4">2.4.2.59</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">thi4</name>
        <name evidence="5" type="ordered locus">Metig_1112</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Involved in the biosynthesis of the thiazole moiety of thiamine. Catalyzes the conversion of NAD and glycine to adenosine diphosphate 5-(2-hydroxyethyl)-4-methylthiazole-2-carboxylate (ADT), an adenylated thiazole intermediate, using free sulfide as a source of sulfur.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>glycine + hydrogen sulfide + NAD(+) = ADP-5-ethyl-4-methylthiazole-2-carboxylate + H(+) + 3 H2O + nicotinamide</text>
            <dbReference type="Rhea" id="RHEA:55704"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17154"/>
            <dbReference type="ChEBI" id="CHEBI:29919"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:139151"/>
            <dbReference type="EC" id="2.4.2.59"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4">Cofactor biosynthesis; thiamine diphosphate biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homooctamer; tetramer of dimers.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the THI4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.59" evidence="4"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96651.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799252.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1112"/>
    <dbReference type="EnsemblBacteria" id="AEF96651">
        <property type="protein sequence ID" value="AEF96651"/>
        <property type="gene ID" value="Metig_1112"/>
    </dbReference>
    <dbReference type="GeneID" id="10643970"/>
    <dbReference type="KEGG" id="mig:Metig_1112"/>
    <dbReference type="HOGENOM" id="CLU_053727_2_0_2"/>
    <dbReference type="OrthoDB" id="61905at2157"/>
    <dbReference type="UniPathway" id="UPA00060"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016763">
        <property type="term" value="F:pentosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009228">
        <property type="term" value="P:thiamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009229">
        <property type="term" value="P:thiamine diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052837">
        <property type="term" value="P:thiazole biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00304">
        <property type="entry name" value="Thi4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002922">
        <property type="entry name" value="Thi4_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022828">
        <property type="entry name" value="Thi4_prok"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00292">
        <property type="entry name" value="TIGR00292"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0408">Iron</keyword>
    <keyword evidence="1 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0520">NAD</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0784">Thiamine biosynthesis</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="binding site" evidence="4">
        <location>
            <position position="40"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="59"/>
            <end position="60"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="67"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="133"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="159"/>
            <end position="161"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <note>ligand shared between two adjacent protomers</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="161"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>ligand shared between two adjacent protomers</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="176"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="179"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="226"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="236"/>
        </location>
        <ligand>
            <name>glycine</name>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEF96651.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="263" mass="28185" checksum="0E43F049AA035EDD" modified="2011-07-27" version="1">MDVRLRADEYATTRAILKSAFDMWLDIIDVDVAIVGGGPSGLTAARYIAKEGYKVVVLERHLAFGGGTWGGGMGFPYIVVEEPADEILREVGVKLEKVEGEDGLYTADSVEVPAKLAVGAIDAGAKVLTGIVVEDLVLRENRVAGVVINSYAIEKAGLHIDPITITAKYVVDATGHDASVVTTLSRKNPELNLEVPGEKSMWAEKGENALLRNTREVYPGLFVCGMAANAVYAGHRMGAIFGGMYISGKKCAEMIVEKLKNNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDU1</accession>
    <name>F6BDU1_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="5">tRNA pseudouridine synthase D TruD</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Metig_1113</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the pseudouridine synthase TruD family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96652.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799253.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1113"/>
    <dbReference type="EnsemblBacteria" id="AEF96652">
        <property type="protein sequence ID" value="AEF96652"/>
        <property type="gene ID" value="Metig_1113"/>
    </dbReference>
    <dbReference type="GeneID" id="10643971"/>
    <dbReference type="KEGG" id="mig:Metig_1113"/>
    <dbReference type="HOGENOM" id="CLU_005281_4_1_2"/>
    <dbReference type="OMA" id="IGYCGLK"/>
    <dbReference type="OrthoDB" id="92795at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009982">
        <property type="term" value="F:pseudouridine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001522">
        <property type="term" value="P:pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2350.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001656">
        <property type="entry name" value="PsdUridine_synth_TruD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020119">
        <property type="entry name" value="PsdUridine_synth_TruD_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011760">
        <property type="entry name" value="PsdUridine_synth_TruD_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042214">
        <property type="entry name" value="TruD_catalytic"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13326">
        <property type="entry name" value="PTHR13326"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01142">
        <property type="entry name" value="TruD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037016">
        <property type="entry name" value="Pseudouridin_synth_euk_prd"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="SSF55120"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00094">
        <property type="entry name" value="tRNA_TruD_broad"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50984">
        <property type="entry name" value="TRUD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01268">
        <property type="entry name" value="UPF0024"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="TRUD" evidence="4">
        <location>
            <begin position="136"/>
            <end position="348"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEF96652.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="400" mass="47221" checksum="91A45D79262C8087" modified="2011-07-27" version="1">MKIRQKPEDFIVEEIIDLGKTINENGEYYLYKLTKRNIENLKALSYIAKKFKIPLKEIGYCGLKDRHALTTQYITIPKKYGILKLDEKNLKLEYVAPSNPLKIGDLIGNRFNITVRSIKSEDFLKIADNIHALDYGVPNYFDSQRFGSVFERKFIAKEIMLGNYEEALKILLTKYKKSEKKTIKDLKRYIAKNWGDWEKCAKYIEKNDIKSRMFVNIINALNKGKSYKEVFKYVDDRLRKIFVSAYQSYLWNECIKEVLKDYVNEKDRYYLEYECGDFMFYKELDEDSFNALKDVKFPTIAPDVEYKGEVKEIIDIVLENEGITFKDLENIDCLDCKFVYSERKILCIPKDFKTSGFKPDELNNGKYKITLEFELNKGSYATMIIKRAFLGVKKSSRRKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-06-02" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDU7</accession>
    <name>F6BDU7_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Tetratricopeptide TPR_3 repeat-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_1119</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96658.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799259.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96658">
        <property type="protein sequence ID" value="AEF96658"/>
        <property type="gene ID" value="Metig_1119"/>
    </dbReference>
    <dbReference type="GeneID" id="10643977"/>
    <dbReference type="KEGG" id="mig:Metig_1119"/>
    <dbReference type="HOGENOM" id="CLU_838405_0_0_2"/>
    <dbReference type="OMA" id="YWLKGCE"/>
    <dbReference type="OrthoDB" id="60899at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011716">
        <property type="entry name" value="TPR-3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07720">
        <property type="entry name" value="TPR_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF96658.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="331" mass="40026" checksum="C74ED8953C0E788D" modified="2011-07-27" version="1">MNYINIKDKNKGWLDALYSAWCNGCKFYNKKEYNKAMKYFNLLNQIFRENVPKYDFYELREEAYKEILNNNIQKGIKKFIWHIEGILLSKDYYSLSGELITVTSILTKIGKYNIAKCFLNIGAERIYEDIKDIKPESILKEVLNSLFGDEEIERIKRENNLENLIKENIIDFNIDYFNSMMDFFMYALNWERFLKIHEEVKNKIKKCKISREIIEKIIERFEESMSDKLSIAYLLKGDYAKCLEYMELFKRHYGECLKEYEIEKNKFNLIEYIANSLKDRIIKKEEWIIELNEIYKDILNKPLKPTYKETKNCDLEYILDYYVLKSFIESI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-06-02" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDV5</accession>
    <name>F6BDV5_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized conserved protein UCP019375</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_1127</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96666.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799267.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96666">
        <property type="protein sequence ID" value="AEF96666"/>
        <property type="gene ID" value="Metig_1127"/>
    </dbReference>
    <dbReference type="GeneID" id="10643985"/>
    <dbReference type="KEGG" id="mig:Metig_1127"/>
    <dbReference type="HOGENOM" id="CLU_539284_0_0_2"/>
    <dbReference type="OMA" id="HHGYMCG"/>
    <dbReference type="OrthoDB" id="13657at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016760">
        <property type="entry name" value="MonoFe_hyd_HmdC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10113">
        <property type="entry name" value="Fibrillarin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF019375">
        <property type="entry name" value="UCP019375"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03958">
        <property type="entry name" value="monoFe_hyd_HmdC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF96666.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="506" mass="56353" checksum="62F0CA752AE28C9A" modified="2011-07-27" version="1">MRDLIKESVNNLDAALELRKLIIKKLNEKKLKESDIIEIVDAVDDLSLNEIQKLGSNLRKFPMGCDLVEVAVGPCASSLTLTQFIENCILTDYMGLTIHVCAYALADIGEKEGIPPLEVMKMVYENVDVPLDLDHFGQYGAMRFPKEITHCMGECYYNGPPYKGCPRERIHKRLIDKEKEYAHEFDDWVKLASTVCINVVEEQGGEEHAAPLEEMAVVAEAAKKYGKGLEGIFHIGDGYDDLITGLKSCIDFDVDVLVVEGAPFNRAKNRLKAFAKAIAVSRILVKGGVVATNGAYEDECRIGLRSGLNVIISGFSGNHHGYMCGYSPGTAKRGNFGLPRVMRIIKEEIKNMDVNLVSRHDLIAIARSSKFLGNIIYPETLGGMFIGDAHWVAIKNSKLHDRVRIRKTLEDVENEYNGEKLGFLGGRYIAWGIAKKLMPEEVYVSDANKWVEEATVKILNEVGINAYKCNGNDEEVVKNADKTYICSMIPEIILKIKNKVDAESLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>F6BE15</accession>
    <name>F6BE15_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">IMP cyclohydrolase</fullName>
            <ecNumber evidence="3">3.5.4.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">IMP synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="3">Inosinicase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">purO</name>
        <name evidence="5" type="ordered locus">Metig_1188</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the cyclization of 5-formylamidoimidazole-4-carboxamide ribonucleotide to IMP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>H2O + IMP = 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide</text>
            <dbReference type="Rhea" id="RHEA:18445"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <dbReference type="ChEBI" id="CHEBI:58467"/>
            <dbReference type="EC" id="3.5.4.10"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Purine metabolism; IMP biosynthesis via de novo pathway; IMP from 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the archaeal IMP cyclohydrolase family.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.10" evidence="3"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96726.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799325.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1188"/>
    <dbReference type="EnsemblBacteria" id="AEF96726">
        <property type="protein sequence ID" value="AEF96726"/>
        <property type="gene ID" value="Metig_1188"/>
    </dbReference>
    <dbReference type="GeneID" id="10644048"/>
    <dbReference type="KEGG" id="mig:Metig_1188"/>
    <dbReference type="HOGENOM" id="CLU_1352116_0_0_2"/>
    <dbReference type="OMA" id="HVDPIAE"/>
    <dbReference type="OrthoDB" id="106750at2157"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00135"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003937">
        <property type="term" value="F:IMP cyclohydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00705">
        <property type="entry name" value="IMP_cyclohydrol"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010191">
        <property type="entry name" value="IMP_cyclohydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020600">
        <property type="entry name" value="IMP_cyclohydrolase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036795">
        <property type="entry name" value="IMP_cyclohydrolase-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07826">
        <property type="entry name" value="IMP_cyclohyd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004866">
        <property type="entry name" value="IMP_cclhdr_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75569">
        <property type="entry name" value="SSF75569"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01922">
        <property type="entry name" value="purO_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 3" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="IMP_cyclohyd" evidence="4">
        <location>
            <begin position="2"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF07826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEF96726.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="202" mass="23150" checksum="9C4071ACD2DD12D0" modified="2011-07-27" version="1">MYIGRFLVVGKTKEGKPFVCYRVSSRSFPNRVAKIIDENTVAIIPKDPNEVFKNPYIAYNCIKIVNNTVVATNGSHTDFIAEKLAFNYPKRDALIYPLATLDYEKDAYNTPRIAAILDREECYMGYVRDDELRVKKVELKEGKGYYLSTYECCAISEEQVIDIEGETPEEICEYILNYKEFEHPVTCATAVIDKDKIKIAIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>F6BE33</accession>
    <name>F6BE33_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Tritrans,polycis-undecaprenyl-diphosphate synthase (geranylgeranyl-diphosphate specific)</fullName>
            <ecNumber evidence="2">2.5.1.89</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">Undecaprenyl diphosphate synthase</fullName>
            <shortName evidence="2">UDS</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="2">Undecaprenyl pyrophosphate synthase</fullName>
            <shortName evidence="2">UPP synthase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2" type="primary">uppS</name>
        <name evidence="3" type="ordered locus">Metig_0007</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the sequential condensation of isopentenyl diphosphate (IPP) with geranylgeranyl diphosphate (GGPP) to yield (2Z,6Z,10Z,14Z,18Z,22Z,26Z,30E,34E,38E)-undecaprenyl diphosphate (tritrans,heptacis-UPP). It is probably the precursor of glycosyl carrier lipids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>geranylgeranyl diphosphate + 7 isopentenyl diphosphate = 7 diphosphate + tri-trans,hepta-cis-undecaprenyl diphosphate</text>
            <dbReference type="Rhea" id="RHEA:27622"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57533"/>
            <dbReference type="ChEBI" id="CHEBI:60388"/>
            <dbReference type="ChEBI" id="CHEBI:128769"/>
            <dbReference type="EC" id="2.5.1.89"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="2">Binds 2 magnesium ions per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPP synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.89" evidence="2"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95569.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0007"/>
    <dbReference type="EnsemblBacteria" id="AEF95569">
        <property type="protein sequence ID" value="AEF95569"/>
        <property type="gene ID" value="Metig_0007"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0007"/>
    <dbReference type="HOGENOM" id="CLU_038505_2_0_2"/>
    <dbReference type="OMA" id="PRTEGHK"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004659">
        <property type="term" value="F:prenyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00475">
        <property type="entry name" value="Cis_IPPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1180.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01139">
        <property type="entry name" value="ISPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001441">
        <property type="entry name" value="UPP_synth-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018520">
        <property type="entry name" value="UPP_synth-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036424">
        <property type="entry name" value="UPP_synth-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10291">
        <property type="entry name" value="PTHR10291"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01255">
        <property type="entry name" value="Prenyltransf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64005">
        <property type="entry name" value="SSF64005"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00055">
        <property type="entry name" value="uppS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01066">
        <property type="entry name" value="UPP_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2 3" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Substrate binding" evidence="2">
        <location>
            <begin position="43"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="2">
        <location>
            <begin position="87"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="2">
        <location>
            <begin position="220"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="active site" evidence="2">
        <location>
            <position position="42"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="2">
        <location>
            <position position="90"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="42"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="59"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="91"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="93"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="214"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="233"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF95569.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="265" mass="31546" checksum="E5BFB585774A2BB1" modified="2011-07-27" version="1">MFLLIKLYNLLSKMGILKIYEKILEKEIDRSRVPKHVGIIMDGNRRTAKELKKPVTYGHYLGAEKVREVLKWCIDLDIKIVTVYAFSMENFRRPKEEVKALMELFKKKFIEVAKDEDIHKYKVRVRAIGRINKLPKDVQDAIKYAEEKTKNYSNYFLNIAIAYGGQQEIVDAVKKIAKKIKNNELDIEDITEETISKHLYTANLPYPNPDLIIRTSGEERISNFLTWQSSYSELYFCETYWPLFRKIDFLRAIRDYQRRERRFGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>F6BE49</accession>
    <name>F6BE49_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Peptidase M50</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Metig_0023</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95585.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798195.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0023"/>
    <dbReference type="EnsemblBacteria" id="AEF95585">
        <property type="protein sequence ID" value="AEF95585"/>
        <property type="gene ID" value="Metig_0023"/>
    </dbReference>
    <dbReference type="GeneID" id="10642858"/>
    <dbReference type="KEGG" id="mig:Metig_0023"/>
    <dbReference type="HOGENOM" id="CLU_042134_1_0_2"/>
    <dbReference type="OMA" id="TIPLWYG"/>
    <dbReference type="OrthoDB" id="94875at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001193">
        <property type="entry name" value="MBTPS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008915">
        <property type="entry name" value="Peptidase_M50"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13325">
        <property type="entry name" value="PTHR13325"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13180">
        <property type="entry name" value="PDZ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02163">
        <property type="entry name" value="Peptidase_M50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="SSF50156"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="6"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="59"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="99"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="140"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="177"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="351"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_M50" evidence="6">
        <location>
            <begin position="111"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="7">
        <location>
            <begin position="207"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF13180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEF95585.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="371" mass="41560" checksum="368A479F5CBCB049" modified="2011-07-27" version="1">MEITSRIILVIALLIWIFLYAIRDSINLKTYFGVFGILRTKVGMRTIEKLGNYEFWKKLAILSIPICVIIGFFTFSSLIDSTIKLLSGELPKEASKPIIFLFGSVIPWIPGIVGLIVGVTVHELAHGIVAYAFRQQIKSTGLLLLLGIPLGAFVELGEEFKNADKKIRGAIASAGPMANIIVFLAVVLITPQLYAIPTELTITKTYASPAMGVLKEGDVLYSINGEKINSLKDFYNMAKNLKPNEEITITVLRGNELKTFNLITSNEGKIGIVVEPSKNLAFVLNCCYWTYWMNLLLGFFNLLPALPLDGYYVWAAFPRVIRDLKLKIKSLETLVEHISNVLEMILNEKNLNTISILIWWIIFGSMIYSFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>F6BE75</accession>
    <name>F6BE75_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Cyclophil_like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1214</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96752.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799351.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96752">
        <property type="protein sequence ID" value="AEF96752"/>
        <property type="gene ID" value="Metig_1214"/>
    </dbReference>
    <dbReference type="GeneID" id="10644074"/>
    <dbReference type="KEGG" id="mig:Metig_1214"/>
    <dbReference type="HOGENOM" id="CLU_144084_1_0_2"/>
    <dbReference type="OMA" id="DEIYFET"/>
    <dbReference type="OrthoDB" id="118310at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029000">
        <property type="entry name" value="Cyclophilin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025658">
        <property type="entry name" value="Cyclophilin_TM1367"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007256">
        <property type="entry name" value="DUF369"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04126">
        <property type="entry name" value="Cyclophil_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006456">
        <property type="entry name" value="UCP006456"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50891">
        <property type="entry name" value="SSF50891"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclophil_like" evidence="1">
        <location>
            <begin position="1"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96752.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="123" mass="13913" checksum="DE675976902A22B6" modified="2011-07-27" version="1">MKIKIKVKDFEVEAELYEDLAPKTVSAIAKALPIKGVVNRWGDEIYFETDVVVNEEENSKEYVELGDIAYWIPGRAICIFFGKTSISDGDKIKPASAVNVIGKVKDWEKFKDVWDGEVIEITK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>F6BE88</accession>
    <name>F6BE88_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Peptidase dimerization domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1228</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96765.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799364.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1228"/>
    <dbReference type="EnsemblBacteria" id="AEF96765">
        <property type="protein sequence ID" value="AEF96765"/>
        <property type="gene ID" value="Metig_1228"/>
    </dbReference>
    <dbReference type="GeneID" id="10644089"/>
    <dbReference type="KEGG" id="mig:Metig_1228"/>
    <dbReference type="HOGENOM" id="CLU_800794_0_0_2"/>
    <dbReference type="OMA" id="VSGHKGM"/>
    <dbReference type="OrthoDB" id="87784at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036264">
        <property type="entry name" value="Bact_exopeptidase_dim_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002933">
        <property type="entry name" value="Peptidase_M20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011650">
        <property type="entry name" value="Peptidase_M20_dimer"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07687">
        <property type="entry name" value="M20_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01546">
        <property type="entry name" value="Peptidase_M20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55031">
        <property type="entry name" value="SSF55031"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="M20_dimer" evidence="1">
        <location>
            <begin position="156"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96765.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="355" mass="40683" checksum="A786D9E4A9BA655D" modified="2011-07-27" version="1">MTPKEFNPIELWEDLSKIRTDNEEGVKKAFKYLSNFFDNLGIKNRIIKGCFVAYRDFEDLQNGVLLNSHIDTVKVLREFEKDDENFYGTGVCDAKAPLVSLVHAFLNNENAVLVVSPDEERDSCGIYNFCHWLKENTKTKKFYCIVGEPTDLKVCIGHKGRFEVVVKAYGKSGHASDPNNGINAIEIIVDVICDLRNFPCSFIEVNGKKYYSSITPTMIKGGIQPNIIPNRAEVILDVRSVEENLMEKIKDYLKEKRYSKYVEVYLNKRKVPYADFYILKNEELLNSISKDFEIDFFNATCEAFYFGNILNADVLIFGAGSLDVIHSPNEFLSIKEFEKGLKMADDLLNRILDFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>F6BE89</accession>
    <name>F6BE89_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Signal recognition particle 54 kDa protein</fullName>
            <shortName evidence="8">SRP54</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">srp54</name>
        <name evidence="10" type="ordered locus">Metig_1229</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Involved in targeting and insertion of nascent membrane proteins into the cytoplasmic membrane. Binds to the hydrophobic signal sequence of the ribosome-nascent chain (RNC) as it emerges from the ribosomes. The SRP-RNC complex is then targeted to the cytoplasmic membrane where it interacts with the SRP receptor FtsY.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Part of the signal recognition particle protein translocation system, which is composed of SRP and FtsY. Archaeal SRP consists of a 7S RNA molecule of 300 nucleotides and two protein subunits: SRP54 and SRP19.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
        <text evidence="8">The SRP-RNC complex is targeted to the cytoplasmic membrane.</text>
    </comment>
    <comment type="domain">
        <text evidence="8">Composed of three domains: the N-terminal N domain, which is responsible for interactions with the ribosome, the central G domain, which binds GTP, and the C-terminal M domain, which binds the RNA and the signal sequence of the RNC.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the GTP-binding SRP family. SRP54 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96766.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799365.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1229"/>
    <dbReference type="EnsemblBacteria" id="AEF96766">
        <property type="protein sequence ID" value="AEF96766"/>
        <property type="gene ID" value="Metig_1229"/>
    </dbReference>
    <dbReference type="GeneID" id="10644090"/>
    <dbReference type="KEGG" id="mig:Metig_1229"/>
    <dbReference type="HOGENOM" id="CLU_009301_6_0_2"/>
    <dbReference type="OMA" id="DTAGRHK"/>
    <dbReference type="OrthoDB" id="25871at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048500">
        <property type="term" value="C:signal recognition particle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008312">
        <property type="term" value="F:7S RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006614">
        <property type="term" value="P:SRP-dependent cotranslational protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00306">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036891">
        <property type="entry name" value="Signal_recog_part_SRP54_M_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013822">
        <property type="entry name" value="Signal_recog_particl_SRP54_hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004125">
        <property type="entry name" value="Signal_recog_particle_SRP54_M"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036225">
        <property type="entry name" value="SRP/SRP_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022941">
        <property type="entry name" value="SRP54"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000897">
        <property type="entry name" value="SRP54_GTPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042101">
        <property type="entry name" value="SRP54_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11564">
        <property type="entry name" value="PTHR11564"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00448">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02881">
        <property type="entry name" value="SRP54_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02978">
        <property type="entry name" value="SRP_SPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00962">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00963">
        <property type="entry name" value="SRP54_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47364">
        <property type="entry name" value="SSF47364"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47446">
        <property type="entry name" value="SSF47446"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00300">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 8" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 8" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 8" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6 8" id="KW-0733">Signal recognition particle</keyword>
    <feature type="domain" description="SRP54" evidence="9">
        <location>
            <begin position="268"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="107"/>
            <end position="114"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="188"/>
            <end position="192"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="247"/>
            <end position="250"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS00300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AEF96766.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="451" mass="50219" checksum="35B0DCC723B8837C" modified="2011-07-27" version="1">MLEKLGESINKALNKIKNATFVDKKLVKEVIKDIQKALIQADVNVKLVLQMSKTIEKRAIEETPPKGFSKKEHIIKIVYEELVKLLGEEPKPLELNPKKQTVILLVGIQGSGKTTTAAKLARYIQKRGLKPALIAADTYRPAAYEQLKQLAEKIHVPLYGDETRTKSPIDIAKEGLEKFKKADVIIIDTAGRHKEEKGLLEEMKMMKEITNPDEIILVIDGTLGQQARNQAKAFKDAVGDIGSIIVTKLDGSAKGGGALSAVAEIKAPIKFIGTGEGIDDLEPFDPKKFISRLLGMGDLDSLLEKADELVDEKTEESIDAILRGKFTLNELYAQLEAINKMGPIKQIMNMIPGFGAGLPKEVVQITEQKLKKYRIIMDSMTKEEKENPEIIKASRIERIARGAGVKPEEVKELLRYYATTKRAIENLKRGKMLKMGGPIGQIMRQLMFKEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEA0</accession>
    <name>F6BEA0_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="9 12">Tetrahydromethanopterin S-methyltransferase subunit C</fullName>
            <ecNumber evidence="8 12">2.1.1.86</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11 12">N5-methyltetrahydromethanopterin--coenzyme M methyltransferase subunit C</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">mtrC</name>
        <name evidence="13" type="ordered locus">Metig_1240</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 12">Part of a complex that catalyzes the formation of methyl-coenzyme M and tetrahydromethanopterin from coenzyme M and methyl-tetrahydromethanopterin. This is an energy-conserving, sodium-ion translocating step.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 12">
            <text>5-methyl-5,6,7,8-tetrahydromethanopterin + coenzyme M + 2 Na(+)(in) = 5,6,7,8-tetrahydromethanopterin + methyl-coenzyme M + 2 Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:53492"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:58103"/>
            <dbReference type="ChEBI" id="CHEBI:58116"/>
            <dbReference type="ChEBI" id="CHEBI:58286"/>
            <dbReference type="ChEBI" id="CHEBI:58319"/>
            <dbReference type="EC" id="2.1.1.86"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="5 12">One-carbon metabolism; methanogenesis from CO(2); methyl-coenzyme M from 5,10-methylene-5,6,7,8-tetrahydromethanopterin: step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7 12">The complex is composed of 8 subunits; MtrA, MtrB, MtrC, MtrD, MtrE, MtrF, MtrG and MtrH.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4 12">Cell membrane</location>
            <topology evidence="4 12">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6 12">Belongs to the MtrC family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.86" evidence="8 12"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96777.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799376.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1240"/>
    <dbReference type="EnsemblBacteria" id="AEF96777">
        <property type="protein sequence ID" value="AEF96777"/>
        <property type="gene ID" value="Metig_1240"/>
    </dbReference>
    <dbReference type="GeneID" id="10644103"/>
    <dbReference type="KEGG" id="mig:Metig_1240"/>
    <dbReference type="HOGENOM" id="CLU_092286_0_0_2"/>
    <dbReference type="OMA" id="HPFNACL"/>
    <dbReference type="OrthoDB" id="61293at2157"/>
    <dbReference type="UniPathway" id="UPA00640">
        <property type="reaction ID" value="UER00698"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030269">
        <property type="term" value="F:tetrahydromethanopterin S-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019386">
        <property type="term" value="P:methanogenesis, from carbon dioxide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01096">
        <property type="entry name" value="MtrC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005865">
        <property type="entry name" value="THM_MeTrfase_su_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04211">
        <property type="entry name" value="MtrC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006530">
        <property type="entry name" value="MtrC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01148">
        <property type="entry name" value="mtrC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="10 12" id="KW-0484">Methanogenesis</keyword>
    <keyword evidence="12 13" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="12" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12 13" id="KW-0808">Transferase</keyword>
    <keyword evidence="12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="42"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="71"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="98"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="140"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="173"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="205"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00007607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00011616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00012514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00015131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00029817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AEF96777.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="264" mass="27022" checksum="9761CA592A198B6C" modified="2011-07-27" version="1">MAHGGGGHAAEVYPENQVLAVGAILAIVGMYVAHFVPSLSMLIGGLLAAGACVAGANTVRKVAAYGLGTGVPSIGMISLGMGTMAALAGVLIPKVFGIPEIVTPILAAVIAIVVGFIIGRLTVNPVGMKIPIMVQSMTKLSLAGALAILGFCVAYAGGFSADVFIDNAVKNGIIALAFIGAGMSILHPFNACLGPNESHERTLTLAVACGLITWLVFAIAKLDLISTIVAAILWAIAYGTYVKMSLRDACCVLYTPELPKKEEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEA7</accession>
    <name>F6BEA7_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CRISPR system single-strand-specific deoxyribonuclease Cas10/Csm1 (subtype III-A)</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Cyclic oligoadenylate synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">Metig_1247</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CRISPR-associated Cas10/Csm1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96784.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1247"/>
    <dbReference type="EnsemblBacteria" id="AEF96784">
        <property type="protein sequence ID" value="AEF96784"/>
        <property type="gene ID" value="Metig_1247"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_1247"/>
    <dbReference type="HOGENOM" id="CLU_017487_0_0_2"/>
    <dbReference type="OMA" id="PSAFYYS"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004527">
        <property type="term" value="F:exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09680">
        <property type="entry name" value="Cas10_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013408">
        <property type="entry name" value="Cas10/Csm1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041062">
        <property type="entry name" value="Csm1_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000160">
        <property type="entry name" value="GGDEF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006674">
        <property type="entry name" value="HD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18211">
        <property type="entry name" value="Csm1_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01966">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02578">
        <property type="entry name" value="cas_TM1811_Csm1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50887">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0051">Antiviral defense</keyword>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="7" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0540">Nuclease</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GGDEF" evidence="11">
        <location>
            <begin position="553"/>
            <end position="719"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00014333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00032922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AEF96784.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="874" mass="103045" checksum="27CA08650765E3AF" modified="2011-07-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEB6</accession>
    <name>F6BEB6_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Phosphoesterase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1256</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96793.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799391.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1256"/>
    <dbReference type="EnsemblBacteria" id="AEF96793">
        <property type="protein sequence ID" value="AEF96793"/>
        <property type="gene ID" value="Metig_1256"/>
    </dbReference>
    <dbReference type="GeneID" id="10644119"/>
    <dbReference type="KEGG" id="mig:Metig_1256"/>
    <dbReference type="HOGENOM" id="CLU_075478_0_1_2"/>
    <dbReference type="OMA" id="GNHDTML"/>
    <dbReference type="OrthoDB" id="105276at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP_ApaH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004376">
        <property type="entry name" value="CHP00024_Pesterase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024173">
        <property type="entry name" value="Pesterase_MJ0037"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30337:SF5">
        <property type="entry name" value="PTHR30337:SF5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000887">
        <property type="entry name" value="Pesterase_MJ0037"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="SSF56300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00024">
        <property type="entry name" value="SbcD_rel_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metallophos" evidence="1">
        <location>
            <begin position="22"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96793.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="237" mass="27374" checksum="9E83B9BFABC1BC2F" modified="2011-07-27" version="1">MEEKIKLRDFYITSDFCLIIGKVGVIADTHLGFDIYINEKGGNFPQIQKDSITERIDNIIDKYKLKTLVINGDIKHNFELSYNEIEFVKEFLGHLKDRIDLILIKGNHDTFISKVAEELGIEIYEHYKIKNYTITHGHANFKEDESFLILGHEHPSIKLRDEVGAILKLPCYLYNKRYIVLPAFNPLSPGNDLVNNYASSPCIKKDYLDSEVYAITELGLLNFGTLRDLREFVKVNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-06-02" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>F6BED3</accession>
    <name>F6BED3_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Haloacid dehalogenase domain protein hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_1273</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96810.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96810">
        <property type="protein sequence ID" value="AEF96810"/>
        <property type="gene ID" value="Metig_1273"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_1273"/>
    <dbReference type="HOGENOM" id="CLU_142246_0_0_2"/>
    <dbReference type="OMA" id="PYIGIGN"/>
    <dbReference type="OrthoDB" id="96759at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="SSF56784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF96810.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="153" mass="17223" checksum="AC96D599416EF3F4" modified="2011-07-27" version="1">MGESVIIIFDLNGTIATDGIIKDSTKKRIKELRKYHEIFILSADTFGTLKDIEEELGIKGIKVDKEKYGSEKIAKNEILKEIREKYKNEKIIAFGNGANDELLLKNADLGICVLGDEGTWTKTLLNSDIVVKNIDDGLDLILKEKRLKATIRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-06-02" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEI2</accession>
    <name>F6BEI2_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcriptional regulator, AbrB family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0082</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95643.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798252.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF95643">
        <property type="protein sequence ID" value="AEF95643"/>
        <property type="gene ID" value="Metig_0082"/>
    </dbReference>
    <dbReference type="GeneID" id="10642917"/>
    <dbReference type="KEGG" id="mig:Metig_0082"/>
    <dbReference type="HOGENOM" id="CLU_3057120_0_0_2"/>
    <dbReference type="OrthoDB" id="130137at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007159">
        <property type="entry name" value="SpoVT-AbrB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037914">
        <property type="entry name" value="SpoVT-AbrB_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89447">
        <property type="entry name" value="SSF89447"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01439">
        <property type="entry name" value="lp_hng_hel_AbrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51740">
        <property type="entry name" value="SPOVT_ABRB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SpoVT-AbrB" evidence="1">
        <location>
            <begin position="6"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51740"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF95643.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="53" mass="6162" checksum="3A6B74974A3EEB23" modified="2011-07-27" version="1">MNISVSFTQKIDKKGKITIPASIRKKFNLETDDLVEVEIKKVFKKDKCYENAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEJ2</accession>
    <name>F6BEJ2_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">DsrE family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_0092</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95653.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798262.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0092"/>
    <dbReference type="EnsemblBacteria" id="AEF95653">
        <property type="protein sequence ID" value="AEF95653"/>
        <property type="gene ID" value="Metig_0092"/>
    </dbReference>
    <dbReference type="GeneID" id="10642927"/>
    <dbReference type="KEGG" id="mig:Metig_0092"/>
    <dbReference type="HOGENOM" id="CLU_2067822_0_0_2"/>
    <dbReference type="OMA" id="KPPYGSE"/>
    <dbReference type="OrthoDB" id="113602at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027396">
        <property type="entry name" value="DsrEFH-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003787">
        <property type="entry name" value="Sulphur_relay_DsrE/F-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02635">
        <property type="entry name" value="DrsE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75169">
        <property type="entry name" value="SSF75169"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF95653.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="118" mass="13342" checksum="E708A469AA1BD52F" modified="2011-07-27" version="1">MVKSLITLILKPPYGSEDSFAGLMFALSQIASGIIEKSDVVLMHDGVYNAKSNQKPETIEMPSIIDVINNLQTFVCKIYCIEEDLKEREIEDIIDGVEIIKSDKLKDILNEYDEIITF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEK6</accession>
    <name>F6BEK6_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Phosphoesterase PA-phosphatase related protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_0106</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95667.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798276.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0106"/>
    <dbReference type="EnsemblBacteria" id="AEF95667">
        <property type="protein sequence ID" value="AEF95667"/>
        <property type="gene ID" value="Metig_0106"/>
    </dbReference>
    <dbReference type="GeneID" id="10642941"/>
    <dbReference type="KEGG" id="mig:Metig_0106"/>
    <dbReference type="HOGENOM" id="CLU_1521855_0_0_2"/>
    <dbReference type="OMA" id="FWKISMH"/>
    <dbReference type="OrthoDB" id="96272at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036938">
        <property type="entry name" value="P_Acid_Pase_2/haloperoxi_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000326">
        <property type="entry name" value="P_Acid_Pase_2/haloperoxidase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01569">
        <property type="entry name" value="PAP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48317">
        <property type="entry name" value="SSF48317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="38"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="69"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="101"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="136"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="162"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="domain" description="acidPPc" evidence="2">
        <location>
            <begin position="23"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF95667.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="184" mass="21247" checksum="FADFA626815421BC" modified="2011-07-27" version="1">MTLEKNTRFVKFAEIVSKAFPFLAIAAFPCIMSDIKDLVTLALIPAIIWILWAKIRNQKWDIPDRNERMIPLIIMAIYGLILVIIEPSFLSKFYLTNILSILIITSFWKISIHCYGLSAIITALIKSNQICGDLPYIIIFIYLGFLIFTMWSRIYLEKHTPLQVIMGTLLGFGQNLIFLYFFAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEL7</accession>
    <name>F6BEL7_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 13">O-phosphoseryl-tRNA(Sec) selenium transferase</fullName>
            <ecNumber evidence="6 13">2.9.1.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10 13">Selenocysteine synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11 13">Selenocysteinyl-tRNA(Sec) synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12 13">Sep-tRNA:Sec-tRNA synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ordered locus">Metig_1277</name>
    </gene>
    <organism evidence="17">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3 13">Converts O-phosphoseryl-tRNA(Sec) to selenocysteinyl-tRNA(Sec) required for selenoprotein biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 13">
            <text>H2O + O-phospho-L-seryl-tRNA(Sec) + selenophosphate = L-selenocysteinyl-tRNA(Sec) + 2 phosphate</text>
            <dbReference type="Rhea" id="RHEA:25041"/>
            <dbReference type="Rhea" id="RHEA-COMP:9743"/>
            <dbReference type="Rhea" id="RHEA-COMP:9947"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16144"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:78551"/>
            <dbReference type="ChEBI" id="CHEBI:78573"/>
            <dbReference type="EC" id="2.9.1.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 13 15">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4 13">Aminoacyl-tRNA biosynthesis; selenocysteinyl-tRNA(Sec) biosynthesis; selenocysteinyl-tRNA(Sec) from L-seryl-tRNA(Sec) (archaeal/eukaryal route): step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5 13">Belongs to the SepSecS family.</text>
    </comment>
    <dbReference type="EC" id="2.9.1.2" evidence="6 13"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96814.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799412.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1277"/>
    <dbReference type="EnsemblBacteria" id="AEF96814">
        <property type="protein sequence ID" value="AEF96814"/>
        <property type="gene ID" value="Metig_1277"/>
    </dbReference>
    <dbReference type="GeneID" id="10644140"/>
    <dbReference type="KEGG" id="mig:Metig_1277"/>
    <dbReference type="HOGENOM" id="CLU_022508_0_0_2"/>
    <dbReference type="OMA" id="HIINGAY"/>
    <dbReference type="OrthoDB" id="16805at2157"/>
    <dbReference type="UniPathway" id="UPA00906">
        <property type="reaction ID" value="UER00898"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098621">
        <property type="term" value="F:phosphoseryl-selenocysteinyl-tRNA selenium transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097056">
        <property type="term" value="P:selenocysteinyl-tRNA(Sec) biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019872">
        <property type="entry name" value="Sec-tRNA_Se_transferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008829">
        <property type="entry name" value="SepSecS/SepCysS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12944">
        <property type="entry name" value="PTHR12944"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05889">
        <property type="entry name" value="SepSecS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017689">
        <property type="entry name" value="SepSecS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03531">
        <property type="entry name" value="selenium_SpcS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="13 15" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 13 15" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="13" id="KW-0711">Selenium</keyword>
    <keyword evidence="13 16" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 13 15" id="KW-0820">tRNA-binding</keyword>
    <feature type="binding site" evidence="14">
        <location>
            <position position="72"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="94"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="95"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="102"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="266"/>
        </location>
        <ligand>
            <name>tRNA</name>
            <dbReference type="ChEBI" id="CHEBI:17843"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="307"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="May act as a substrate filter by repelling compounds with a negatively charged alpha-carboxylate" evidence="15">
        <location>
            <position position="71"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="15">
        <location>
            <position position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00002552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00007037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00021963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00030669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00032048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00032693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRNR" id="PIRNR017689"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR017689-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR017689-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="AEF96814.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="435" mass="48574" checksum="7F928DD8FEF2CD36" modified="2011-07-27" version="1">MLDLNFTGLIPSNMEKRGELTLKDNLAIIEEIFNQRKIPKEGLDDDKIKLLLKILSLMDTDKDPKVIQIGEREARIASKIQNELVGGFCHGIGRSGNLIDAQPKAPGASIMYALTNKILESFLKNLGLKVNAIATPVATGMSLALCLSAARKKYHSNVVIYPYASHKSPIKATSFIGMRMRLVETKLYGDAVRVDVSDIEDAIKKEIKENNNPCVLSTLTFFPPRESDDIVEISKICEEFSIPHIINGAYAIQNNFYIDKLKKAFKHRVDAVVSSSDKNLFTPIGGGIIYSRDREFLREVSLCYPGRACATPVVNILVSLLSLGMNKYLDLMKKQKESKKLLDDLLNELAKKKEEKVLNVKNPISSCITTKKDPVDVAAKLYNLRITGPRGIRKNDKFGTCYLGEYPYDYIVVNSAIGVKNEDIMQVVEKLEKVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEM8</accession>
    <name>F6BEM8_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Acetylglutamate kinase</fullName>
            <ecNumber evidence="7">2.7.2.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">N-acetyl-L-glutamate 5-phosphotransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">NAG kinase</fullName>
            <shortName evidence="7">NAGK</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">argB</name>
        <name evidence="9" type="ordered locus">Metig_1288</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the ATP-dependent phosphorylation of N-acetyl-L-glutamate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>ATP + N-acetyl-L-glutamate = ADP + N-acetyl-L-glutamyl 5-phosphate</text>
            <dbReference type="Rhea" id="RHEA:14629"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:44337"/>
            <dbReference type="ChEBI" id="CHEBI:57936"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.2.8"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="7">Amino-acid biosynthesis; L-arginine biosynthesis; N(2)-acetyl-L-ornithine from L-glutamate: step 2/4.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the acetylglutamate kinase family. ArgB subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.2.8" evidence="7"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96825.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799422.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1288"/>
    <dbReference type="EnsemblBacteria" id="AEF96825">
        <property type="protein sequence ID" value="AEF96825"/>
        <property type="gene ID" value="Metig_1288"/>
    </dbReference>
    <dbReference type="GeneID" id="10644151"/>
    <dbReference type="KEGG" id="mig:Metig_1288"/>
    <dbReference type="HOGENOM" id="CLU_053680_0_0_2"/>
    <dbReference type="OMA" id="EGLYEDW"/>
    <dbReference type="OrthoDB" id="60029at2157"/>
    <dbReference type="UniPathway" id="UPA00068">
        <property type="reaction ID" value="UER00107"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003991">
        <property type="term" value="F:acetylglutamate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042450">
        <property type="term" value="P:arginine biosynthetic process via ornithine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04250">
        <property type="entry name" value="AAK_NAGK-C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1160.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00082">
        <property type="entry name" value="ArgB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036393">
        <property type="entry name" value="AceGlu_kinase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004662">
        <property type="entry name" value="AcgluKinase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037528">
        <property type="entry name" value="ArgB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001048">
        <property type="entry name" value="Asp/Glu/Uridylate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001057">
        <property type="entry name" value="Glu/AcGlu_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041727">
        <property type="entry name" value="NAGK-C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00696">
        <property type="entry name" value="AA_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000728">
        <property type="entry name" value="NAGK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00474">
        <property type="entry name" value="GLU5KINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53633">
        <property type="entry name" value="SSF53633"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00761">
        <property type="entry name" value="argB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="1 7" id="KW-0055">Arginine biosynthesis</keyword>
    <keyword evidence="6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 7 9" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="AA_kinase" evidence="8">
        <location>
            <begin position="26"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="7">
        <location>
            <begin position="66"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="88"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="191"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="7">
        <location>
            <position position="31"/>
        </location>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="7">
        <location>
            <position position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AEF96825.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="297" mass="32500" checksum="72D6A644CD47C246" modified="2011-07-27" version="1">MEEKTNLSKAEILMEALPYIQKFQNKRFVIKYGGHAMIDENAKNWTAKDIVLLRFVGINPVVVHGGGPEINKAMEKMGKKPEFIHGLRVTDEETLDIVMMVLIGKINGEIVSKIGKFGGKAVGLSGKSGHLIKARKKIKYIEKDGERIMVDLGRVGETERINTELIDILLENNYIPVISPIGIDDEGKALNLNADTVAGDIAGAIGAEKLILITDVDGIMEDINDPSTLYKKLTISEVEELMKKGIIKGGMIPKVEAAINALNKGVKSVHIINGKIPHALLLEIFTEEGIGTMIVRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEP5</accession>
    <name>F6BEP5_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 7">6,7-dimethyl-8-ribityllumazine synthase</fullName>
            <shortName evidence="7">DMRL synthase</shortName>
            <shortName evidence="7">LS</shortName>
            <shortName evidence="7">Lumazine synthase</shortName>
            <ecNumber evidence="4 7">2.5.1.78</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">ribH</name>
        <name evidence="8" type="ordered locus">Metig_1305</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the formation of 6,7-dimethyl-8-ribityllumazine by condensation of 5-amino-6-(D-ribitylamino)uracil with 3,4-dihydroxy-2-butanone 4-phosphate. This is the penultimate step in the biosynthesis of riboflavin.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>(2S)-2-hydroxy-3-oxobutyl phosphate + 5-amino-6-(D-ribitylamino)uracil = 6,7-dimethyl-8-(1-D-ribityl)lumazine + H(+) + 2 H2O + phosphate</text>
            <dbReference type="Rhea" id="RHEA:26152"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15934"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58201"/>
            <dbReference type="ChEBI" id="CHEBI:58830"/>
            <dbReference type="EC" id="2.5.1.78"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 7">Cofactor biosynthesis; riboflavin biosynthesis; riboflavin from 2-hydroxy-3-oxobutyl phosphate and 5-amino-6-(D-ribitylamino)uracil: step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Forms an icosahedral capsid composed of 60 subunits, arranged as a dodecamer of pentamers.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 7">Belongs to the DMRL synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.78" evidence="4 7"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96842.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799439.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1305"/>
    <dbReference type="EnsemblBacteria" id="AEF96842">
        <property type="protein sequence ID" value="AEF96842"/>
        <property type="gene ID" value="Metig_1305"/>
    </dbReference>
    <dbReference type="GeneID" id="10644168"/>
    <dbReference type="KEGG" id="mig:Metig_1305"/>
    <dbReference type="HOGENOM" id="CLU_089358_3_1_2"/>
    <dbReference type="OMA" id="SGPGMTR"/>
    <dbReference type="OrthoDB" id="88531at2157"/>
    <dbReference type="UniPathway" id="UPA00275">
        <property type="reaction ID" value="UER00404"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009349">
        <property type="term" value="C:riboflavin synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000906">
        <property type="term" value="F:6,7-dimethyl-8-ribityllumazine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009231">
        <property type="term" value="P:riboflavin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.960">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00178">
        <property type="entry name" value="Lumazine_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034964">
        <property type="entry name" value="LS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002180">
        <property type="entry name" value="LS/RS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036467">
        <property type="entry name" value="LS/RS_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21058">
        <property type="entry name" value="PTHR21058"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00885">
        <property type="entry name" value="DMRL_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52121">
        <property type="entry name" value="SSF52121"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00114">
        <property type="entry name" value="lumazine-synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0686">Riboflavin biosynthesis</keyword>
    <keyword evidence="6 7 8" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="5-amino-6-(D-ribitylamino)uracil binding" evidence="7">
        <location>
            <begin position="43"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="5-amino-6-(D-ribitylamino)uracil binding" evidence="7">
        <location>
            <begin position="67"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="1-deoxy-L-glycero-tetrulose 4-phosphate binding" evidence="7">
        <location>
            <begin position="72"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="7">
        <location>
            <position position="75"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="11"/>
        </location>
        <ligand>
            <name>5-amino-6-(D-ribitylamino)uracil</name>
            <dbReference type="ChEBI" id="CHEBI:15934"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="100"/>
        </location>
        <ligand>
            <name>5-amino-6-(D-ribitylamino)uracil</name>
            <dbReference type="ChEBI" id="CHEBI:15934"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="109"/>
        </location>
        <ligand>
            <name>(2S)-2-hydroxy-3-oxobutyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58830"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEF96842.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="139" mass="15516" checksum="AD119303557EC9EA" modified="2011-07-27" version="1">MVNLAFVIAEFNREITYMMEKVAEEHAEFLGAKVKYKIVVPGTFDMPLAVKRLLEKEDVDAVVTLGCVIEGETEHDEIVVHNAARKIADLALEYNKPVTLGISGPGMTRLQAEDRIDYGKRAVEAAVKMVKRLKALDEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEP7</accession>
    <name>F6BEP7_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_1307</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96844.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799441.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96844">
        <property type="protein sequence ID" value="AEF96844"/>
        <property type="gene ID" value="Metig_1307"/>
    </dbReference>
    <dbReference type="GeneID" id="10644170"/>
    <dbReference type="KEGG" id="mig:Metig_1307"/>
    <dbReference type="HOGENOM" id="CLU_2461784_0_0_2"/>
    <dbReference type="OrthoDB" id="136892at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF96844.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="88" mass="10324" checksum="68C6CB9E42976894" modified="2011-07-27" version="1">MWVKIGDNHIINLNLMGEFWCDFESSCIAIGETPFENGVKRILLLYFEDEEKMMKAYETLIDGLKSNKNYVDISEHIVNIEEAIYEEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEW8</accession>
    <name>F6BEW8_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0144</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95704.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798313.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF95704">
        <property type="protein sequence ID" value="AEF95704"/>
        <property type="gene ID" value="Metig_0144"/>
    </dbReference>
    <dbReference type="GeneID" id="10642979"/>
    <dbReference type="KEGG" id="mig:Metig_0144"/>
    <dbReference type="HOGENOM" id="CLU_992514_0_0_2"/>
    <dbReference type="OMA" id="WEDNITN"/>
    <dbReference type="OrthoDB" id="143161at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="258"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF95704.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="280" mass="31837" checksum="D5FAB4ED0F64161E" modified="2011-07-27" version="1">MLKKIIFLVGTLLLFSVVYGEIWSDTKYPNDNYTENDINEIFNFPYDFEKIDKIEIKIIAFDVDASGKDYVYANNKKIGYLNYGDGITTFNINSSYIDSNGNIKMKVDKNDKMQINKSIMYVHYYNKTSDKNEIYSQIVKIEDKATKTDKSWTFNFPYYNDINIIINNIVIEIEAFGVNMGEDLVYADNEELGRLLDNNGFSTTTFTINYPKAEDILSDGSITIKVDKSKGDNITVNSITLTVYYTPKSSTSTAKAPIPLGVIALSLIGISFLLVRYYGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEZ6</accession>
    <name>F6BEZ6_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="4">DNA helicase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metig_0175</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DNA2/NAM7 helicase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95732.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798341.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0175"/>
    <dbReference type="EnsemblBacteria" id="AEF95732">
        <property type="protein sequence ID" value="AEF95732"/>
        <property type="gene ID" value="Metig_0175"/>
    </dbReference>
    <dbReference type="GeneID" id="10643010"/>
    <dbReference type="KEGG" id="mig:Metig_0175"/>
    <dbReference type="HOGENOM" id="CLU_001666_8_2_2"/>
    <dbReference type="OMA" id="YANDITF"/>
    <dbReference type="OrthoDB" id="9523at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd18808">
        <property type="entry name" value="SF1_C_Upf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045055">
        <property type="entry name" value="DNA2/NAM7-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041679">
        <property type="entry name" value="DNA2/NAM7-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041677">
        <property type="entry name" value="DNA2/NAM7_AAA_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004483">
        <property type="entry name" value="SMUBP-2/Hcs1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10887">
        <property type="entry name" value="PTHR10887"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13086">
        <property type="entry name" value="AAA_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13087">
        <property type="entry name" value="AAA_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00376">
        <property type="entry name" value="TIGR00376"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0347">Helicase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="3">
        <location>
            <begin position="169"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="333"/>
            <end position="360"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEF95732.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="654" mass="75930" checksum="BFEF78F9FC9AD85C" modified="2011-07-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>F6BF03</accession>
    <name>F6BF03_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Putative HTH-type transcriptional regulatory protein Metig_0182</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metig_0182</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95739.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0182"/>
    <dbReference type="EnsemblBacteria" id="AEF95739">
        <property type="protein sequence ID" value="AEF95739"/>
        <property type="gene ID" value="Metig_0182"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0182"/>
    <dbReference type="HOGENOM" id="CLU_075726_0_0_2"/>
    <dbReference type="OMA" id="PGVVYFR"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00584">
        <property type="entry name" value="HTH_type_cro_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020886">
        <property type="entry name" value="Arc_TR_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01381">
        <property type="entry name" value="HTH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00530">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH cro/C1-type" evidence="3">
        <location>
            <begin position="129"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEF95739.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="327" mass="37579" checksum="B54247F0179F9772" modified="2011-07-27" version="1">MMREILLAECINLLRSHNFNVSQPLGRACFDIIANRGYVRLLIKILKNIDSLSEEQSKELLKIANILSAVPIIIGIRTRNAMMEHGVVYDRYNIKAVTFETFEEYLKGSPPVVYAHRGGFFVKIDGEALKRARERLNVSVGELAEASGVSRKTIYKYEQNKANPSVEVAMRIEEYLDVPLAKGIDLFEPVNVQKDNESQKRGPPFPPDEEINIEEYKKQALNILNELGFNLIPTLRAPFDAVAEKHEEKHNILLTNIGEQENEDIRRRAILVREISKLLDGYSLLILEKKERHYKNLAVISMKELEKMDDALDLIEFIKDMLKSDKW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>F6BF10</accession>
    <name>F6BF10_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Metal dependent phosphohydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0189</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95746.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0189"/>
    <dbReference type="EnsemblBacteria" id="AEF95746">
        <property type="protein sequence ID" value="AEF95746"/>
        <property type="gene ID" value="Metig_0189"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0189"/>
    <dbReference type="HOGENOM" id="CLU_068640_0_0_2"/>
    <dbReference type="OMA" id="LHAILCH"/>
    <dbReference type="OrthoDB" id="51434at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006674">
        <property type="entry name" value="HD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039967">
        <property type="entry name" value="MJ1020-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40517">
        <property type="entry name" value="PTHR40517"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01966">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00471">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51831">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HD" evidence="1">
        <location>
            <begin position="45"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF95746.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="251" mass="28256" checksum="F99582AF7E3BD390" modified="2011-07-27" version="1">MEELQNLEGIPKKIYEEISKDAEIKSLLKMSNIMAVKRMGFNDHGKTHAKIVANNAIKMLKILYKNGIKPSFVRDYNGTFEDSLVIVLLSAYLHDIGNAVHRLNHHFHSTYLARPILERILKKYYKNPTPIITETLHAIYSHNEGIMGQTIEAGVVAVADGTDMTEGRSRVPICKGCYDIHSVSAAAIKEVIIKEGTKKPIHIEVILSNEAGIFQIQEILGEKIKWSGIKDYVSVYAKVIKEKPVFEEITL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>F6BF11</accession>
    <name>F6BF11_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_0190</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95747.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798356.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF95747">
        <property type="protein sequence ID" value="AEF95747"/>
        <property type="gene ID" value="Metig_0190"/>
    </dbReference>
    <dbReference type="GeneID" id="10643025"/>
    <dbReference type="KEGG" id="mig:Metig_0190"/>
    <dbReference type="HOGENOM" id="CLU_194236_0_0_2"/>
    <dbReference type="OMA" id="NARYTMD"/>
    <dbReference type="OrthoDB" id="124040at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF95747.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="61" mass="7183" checksum="F08672DE9D556199" modified="2011-07-27" version="1">MDEIDKKAIEMLLKAPFMTEEEMKNTVKILKQMARMKKNKGSIKEILDYWANQAYILSMEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>F6BF13</accession>
    <name>F6BF13_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein archease</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Metig_0192</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Activates the tRNA-splicing ligase complex by facilitating the enzymatic turnover of catalytic subunit RtcB. Acts by promoting the guanylylation of RtcB, a key intermediate step in tRNA ligation. Can also alter the NTP specificity of RtcB such that ATP, dGTP or ITP is used efficiently.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the archease family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95749.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798358.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0192"/>
    <dbReference type="EnsemblBacteria" id="AEF95749">
        <property type="protein sequence ID" value="AEF95749"/>
        <property type="gene ID" value="Metig_0192"/>
    </dbReference>
    <dbReference type="GeneID" id="10643027"/>
    <dbReference type="KEGG" id="mig:Metig_0192"/>
    <dbReference type="HOGENOM" id="CLU_111362_3_0_2"/>
    <dbReference type="OMA" id="WLSELLY"/>
    <dbReference type="OrthoDB" id="125788at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006388">
        <property type="term" value="P:tRNA splicing, via endonucleolytic cleavage and ligation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.55.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01222">
        <property type="entry name" value="Archease_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002804">
        <property type="entry name" value="Archease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022952">
        <property type="entry name" value="Archease_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023572">
        <property type="entry name" value="Archease_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036820">
        <property type="entry name" value="Archease_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12682">
        <property type="entry name" value="PTHR12682"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01951">
        <property type="entry name" value="Archease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69819">
        <property type="entry name" value="SSF69819"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0106">Calcium</keyword>
    <keyword evidence="3 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="Archease" evidence="6">
        <location>
            <begin position="2"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="10"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="138"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="139"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01951"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEF95749.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="139" mass="16228" checksum="C936E52D4C285FD4" modified="2011-07-27" version="1">MFKYFETTADMGIIAEGKTLEEAFSNAAKGLFNLMVDIEKVEKKEKRTIEVSADNLCDLLCDFLTELLILHDSEFLVFSEFDVKIDKEEETYHLKCTAYGEEFDKEKHEPKEEVKAITYHKMEVKQEGDKWVVKFIVDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>F6BF39</accession>
    <name>F6BF39_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">30S ribosomal protein S6e</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">rps6e</name>
        <name evidence="5" type="ordered locus">Metig_1374</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein eS6 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96909.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799505.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1374"/>
    <dbReference type="EnsemblBacteria" id="AEF96909">
        <property type="protein sequence ID" value="AEF96909"/>
        <property type="gene ID" value="Metig_1374"/>
    </dbReference>
    <dbReference type="GeneID" id="10644239"/>
    <dbReference type="KEGG" id="mig:Metig_1374"/>
    <dbReference type="HOGENOM" id="CLU_109671_1_1_2"/>
    <dbReference type="OMA" id="DGFPMRP"/>
    <dbReference type="OrthoDB" id="112720at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00512">
        <property type="entry name" value="Ribosomal_S6e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001377">
        <property type="entry name" value="Ribosomal_S6e"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020924">
        <property type="entry name" value="Ribosomal_S6e_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018282">
        <property type="entry name" value="Ribosomal_S6e_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11502">
        <property type="entry name" value="PTHR11502"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01092">
        <property type="entry name" value="Ribosomal_S6e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01405">
        <property type="entry name" value="Ribosomal_S6e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00578">
        <property type="entry name" value="RIBOSOMAL_S6E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 5" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEF96909.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="128" mass="13873" checksum="1A98C4424FD255E4" modified="2011-07-27" version="1">MPKFKVVVADPKEGKCYQVEVETDALIGKKIGDVIDGKIVGLEGYKLQITGGSDYCGFPMRPDVHGSGRVRILLSKGPGFRPKEKGERRRKTVRGNTISSDIVQVNTKIVEYGDKPVAELLGLGGGEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-06-02" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>F6BF42</accession>
    <name>F6BF42_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Flagella protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_1377</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96912.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799508.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96912">
        <property type="protein sequence ID" value="AEF96912"/>
        <property type="gene ID" value="Metig_1377"/>
    </dbReference>
    <dbReference type="GeneID" id="10644250"/>
    <dbReference type="KEGG" id="mig:Metig_1377"/>
    <dbReference type="HOGENOM" id="CLU_1559494_0_0_2"/>
    <dbReference type="OMA" id="LEYLCER"/>
    <dbReference type="OrthoDB" id="61570at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097588">
        <property type="term" value="P:archaeal or bacterial-type flagellum-dependent cell motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006752">
        <property type="entry name" value="Arch_fla_DE"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04659">
        <property type="entry name" value="Arch_fla_DE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0966">Cell projection</keyword>
    <keyword evidence="3" id="KW-0969">Cilium</keyword>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0282">Flagellum</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Arch_fla_DE" evidence="2">
        <location>
            <begin position="25"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="128"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF96912.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="171" mass="20199" checksum="F1C67A74B6BCD398" modified="2011-07-27" version="1">MKLITKEYYKEPHGINFMFDILEGNYLLTSLNDDAKTMAILTKWIEYLISHVGKSKAEDILIYYNEINWISDKVLMKLLKILEHTNFDKKFDKKDGESLELKSIVNIHIISLYYITYLSGRPLSFDDIDEIKLELIKLKKFLENIKEMTNDLNKNEKNSMFANLEETNSLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F6BF52</accession>
    <name>F6BF52_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Cupin 2 conserved barrel domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1387</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96922.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1387"/>
    <dbReference type="EnsemblBacteria" id="AEF96922">
        <property type="protein sequence ID" value="AEF96922"/>
        <property type="gene ID" value="Metig_1387"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_1387"/>
    <dbReference type="HOGENOM" id="CLU_129810_1_1_2"/>
    <dbReference type="OMA" id="PLKILCC"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013096">
        <property type="entry name" value="Cupin_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07883">
        <property type="entry name" value="Cupin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="SSF51182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cupin_2" evidence="1">
        <location>
            <begin position="45"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96922.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="125" mass="14171" checksum="DD7C7804F8F4069A" modified="2011-07-27" version="1">MIIMKIIKSEYDKIKPYVTKDGSIIRELMHPSVHGDVKQSLAEAIVPVGSKTLLHKHHESEEIYYILEGKGLMTLGNEKFEVKKGDTICIPPETPHKIENIGKVPLKILCCSFPPYSHEDTELME</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>F6BF72</accession>
    <name>F6BF72_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Methyltransferase type 11</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1407</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96942.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96942">
        <property type="protein sequence ID" value="AEF96942"/>
        <property type="gene ID" value="Metig_1407"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_1407"/>
    <dbReference type="HOGENOM" id="CLU_037990_4_0_2"/>
    <dbReference type="OMA" id="WDESSAT"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013216">
        <property type="entry name" value="Methyltransf_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08241">
        <property type="entry name" value="Methyltransf_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransf_11" evidence="1">
        <location>
            <begin position="93"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96942.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="293" mass="34026" checksum="6114FCE9E347D89B" modified="2011-07-27" version="1">MFLIYGVFKDFFPNYTKTCYKTVKYKYQILPIMEIIVINNINCDKMVNNIKEEIRAYWDFRSDSYDNSPGHSGFVDIWKTVLSEIFKRKMKILDVGCGTGFLSLILAELGHEVVGIDLSEGMLNKAREKAKNLGLDIEFMVGDAENLPFEDNTFDAVVNRHLLWTLPNPDKAIMEWARVVKRGGKVVVIDGKWRKNSFNESLRTIFRYLAVAIYEKRNPFQNMGYKEEVAKMLPFNGGTDAENIVKLFKNANLDEIKVNDLSWIKETQKKNLPLLYRLAWGNTHYYLVEGFKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAC0</accession>
    <name>F6BAC0_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Probable phosphoheptose isomerase</fullName>
            <ecNumber evidence="9">5.3.1.28</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Sedoheptulose 7-phosphate isomerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">gmhA</name>
        <name evidence="11" type="ordered locus">Metig_0254</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the isomerization of sedoheptulose 7-phosphate in D-glycero-D-manno-heptose 7-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>2 D-sedoheptulose 7-phosphate = D-glycero-alpha-D-manno-heptose 7-phosphate + D-glycero-beta-D-manno-heptose 7-phosphate</text>
            <dbReference type="Rhea" id="RHEA:27489"/>
            <dbReference type="ChEBI" id="CHEBI:57483"/>
            <dbReference type="ChEBI" id="CHEBI:60203"/>
            <dbReference type="ChEBI" id="CHEBI:60204"/>
            <dbReference type="EC" id="5.3.1.28"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="9">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="9">Carbohydrate biosynthesis; D-glycero-D-manno-heptose 7-phosphate biosynthesis; D-glycero-alpha-D-manno-heptose 7-phosphate and D-glycero-beta-D-manno-heptose 7-phosphate from sedoheptulose 7-phosphate: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="miscellaneous">
        <text evidence="9">The reaction produces a racemic mixture of D-glycero-alpha-D-manno-heptose 7-phosphate and D-glycero-beta-D-manno-heptose 7-phosphate.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the SIS family. GmhA subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.28" evidence="9"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95810.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798419.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0254"/>
    <dbReference type="EnsemblBacteria" id="AEF95810">
        <property type="protein sequence ID" value="AEF95810"/>
        <property type="gene ID" value="Metig_0254"/>
    </dbReference>
    <dbReference type="GeneID" id="10643089"/>
    <dbReference type="KEGG" id="mig:Metig_0254"/>
    <dbReference type="HOGENOM" id="CLU_080999_4_0_2"/>
    <dbReference type="OMA" id="DVHICVP"/>
    <dbReference type="OrthoDB" id="91374at2157"/>
    <dbReference type="UniPathway" id="UPA00041">
        <property type="reaction ID" value="UER00436"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097367">
        <property type="term" value="F:carbohydrate derivative binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008968">
        <property type="term" value="F:D-sedoheptulose 7-phosphate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001061">
        <property type="term" value="P:D-glycero-D-manno-heptose 7-phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd05006">
        <property type="entry name" value="SIS_GmhA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00067">
        <property type="entry name" value="GmhA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035461">
        <property type="entry name" value="GmhA/DiaA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004515">
        <property type="entry name" value="Phosphoheptose_Isoase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001347">
        <property type="entry name" value="SIS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046348">
        <property type="entry name" value="SIS_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13580">
        <property type="entry name" value="SIS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53697">
        <property type="entry name" value="SSF53697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00441">
        <property type="entry name" value="gmhA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51464">
        <property type="entry name" value="SIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="4 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 9 11" id="KW-0413">Isomerase</keyword>
    <keyword evidence="5 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="SIS" evidence="10">
        <location>
            <begin position="30"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="9">
        <location>
            <begin position="45"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="9">
        <location>
            <begin position="87"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="9">
        <location>
            <begin position="113"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="54"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="58"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="118"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="165"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="173"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000348"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AEF95810.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="185" mass="20254" checksum="0D19C0BD27AE1FEF" modified="2011-07-27" version="1">MKEYFEESAKVKLKFIEENEENLRKSIEIIAEVLKNGNKILICGNGGSAADAQHFAAEIVGRFKLERKGLPAIALTTDTSILTAIGNDYGFEMIFERQVEALGKKGDVLVGISTSGNSKNVIRAVNKAKEMGIYTIGLLGKGGGKLKDIVDLALVVPSNDTARIQECHLTIYHVICEEVEKRLFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAC2</accession>
    <name>F6BAC2_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Metig_0256</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SEC61-beta family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95812.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798421.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0256"/>
    <dbReference type="EnsemblBacteria" id="AEF95812">
        <property type="protein sequence ID" value="AEF95812"/>
        <property type="gene ID" value="Metig_0256"/>
    </dbReference>
    <dbReference type="GeneID" id="10643091"/>
    <dbReference type="KEGG" id="mig:Metig_0256"/>
    <dbReference type="HOGENOM" id="CLU_3057121_0_0_2"/>
    <dbReference type="OMA" id="GLIRYMD"/>
    <dbReference type="OrthoDB" id="127915at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016482">
        <property type="entry name" value="SecG/Sec61-beta/Sbh"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03911">
        <property type="entry name" value="Sec61_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0811">Translocation</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="33"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AEF95812.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="53" mass="5957" checksum="9362FE9D88A5CF82" modified="2011-07-27" version="1">MSKREDRGLATSAGLVRYMDESLSKIRIEPEKVIGITLFIIVAEIILTYGVFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAD0</accession>
    <name>F6BAD0_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">TIM-barrel protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0264</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95820.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798429.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0264"/>
    <dbReference type="EnsemblBacteria" id="AEF95820">
        <property type="protein sequence ID" value="AEF95820"/>
        <property type="gene ID" value="Metig_0264"/>
    </dbReference>
    <dbReference type="GeneID" id="10643099"/>
    <dbReference type="KEGG" id="mig:Metig_0264"/>
    <dbReference type="HOGENOM" id="CLU_1118194_0_0_2"/>
    <dbReference type="OMA" id="ELNCHCR"/>
    <dbReference type="OrthoDB" id="72918at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035587">
        <property type="entry name" value="DUS-like_FMN-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005270">
        <property type="entry name" value="tRNA_dU_NifR3-rel"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01207">
        <property type="entry name" value="Dus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00736">
        <property type="entry name" value="nifR3_rel_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dus" evidence="1">
        <location>
            <begin position="89"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF95820.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="260" mass="29805" checksum="0017ED0B857A1248" modified="2011-07-27" version="1">MGKDEKIKKIKNLNKKIVLAPMAGITNGDFCKRFKGLFGIVTIGAYSLDKDSLNASREIVKRGREEFIYNLDDFGDIIRDEIKKARESNALVSVNVRFKNLEESLERLKIISKHADIIELNCHCRQKEITNLGIGQELLKNKELLRSYLIGMADLNVPIFLKVRANFVGLEELIETLNYTRDYFDGIHIDCFNPGKDYADLEVLKEISKNFSDKIIIGNNSVKSIEDAKNMLRYANFVSVARCILRGDIEWIKEFNAMEM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAE7</accession>
    <name>F6BAE7_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 6">30S ribosomal protein S8e</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">rps8e</name>
        <name evidence="8" type="ordered locus">Metig_1425</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="2 6">Part of the 30S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the eukaryotic ribosomal protein eS8 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96960.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799556.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1425"/>
    <dbReference type="EnsemblBacteria" id="AEF96960">
        <property type="protein sequence ID" value="AEF96960"/>
        <property type="gene ID" value="Metig_1425"/>
    </dbReference>
    <dbReference type="GeneID" id="10644298"/>
    <dbReference type="KEGG" id="mig:Metig_1425"/>
    <dbReference type="HOGENOM" id="CLU_080597_2_1_2"/>
    <dbReference type="OMA" id="NPNYVRR"/>
    <dbReference type="OrthoDB" id="116294at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00029">
        <property type="entry name" value="Ribosomal_S8e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020919">
        <property type="entry name" value="Ribosomal_protein_S8e_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001047">
        <property type="entry name" value="Ribosomal_S8e"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022309">
        <property type="entry name" value="Ribosomal_S8e/biogenesis_NSA2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018283">
        <property type="entry name" value="Ribosomal_S8e_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10394">
        <property type="entry name" value="PTHR10394"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01201">
        <property type="entry name" value="Ribosomal_S8e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00307">
        <property type="entry name" value="eS8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01193">
        <property type="entry name" value="RIBOSOMAL_S8E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 6 8" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00029532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEF96960.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="129" mass="14649" checksum="AC5FCE3CD3B40BCF" modified="2011-07-27" version="1">MGVWQGKSRRKPTGGKYKMARKKRKYEMGREPTETLLSEEIRMKIIRCRGGNRKVRLLRTNYANVLDPKTGVCKKVAIKTVVDNQANKHYIRRNIITKGAIIETELGLAKVTSRPGQDGVVNAVLIEQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAI0</accession>
    <name>F6BAI0_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcriptional regulator, ArsR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1458</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96993.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799589.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1458"/>
    <dbReference type="EnsemblBacteria" id="AEF96993">
        <property type="protein sequence ID" value="AEF96993"/>
        <property type="gene ID" value="Metig_1458"/>
    </dbReference>
    <dbReference type="GeneID" id="10644331"/>
    <dbReference type="KEGG" id="mig:Metig_1458"/>
    <dbReference type="HOGENOM" id="CLU_107445_0_0_2"/>
    <dbReference type="OMA" id="KVWIKGE"/>
    <dbReference type="OrthoDB" id="106602at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001845">
        <property type="entry name" value="HTH_ArsR_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026282">
        <property type="entry name" value="MJ1563"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01022">
        <property type="entry name" value="HTH_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006707">
        <property type="entry name" value="MJ1563"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50987">
        <property type="entry name" value="HTH_ARSR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH arsR-type" evidence="1">
        <location>
            <begin position="4"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96993.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="149" mass="17185" checksum="3F50559A8DE96550" modified="2011-07-27" version="1">MEEVKRAVIELFSEIAKIHGLNKSVGGVYAILYLSDKPLCIADIMDELGISKGNVSMALNKLEELGFVRKVWIKGKRRNYYEAMDGFSSYKDIVKKKHDVITKTYEKLKELEQKSEGEEKEFIKRKIKGIERMKKVSEKVLELLNKLEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAI5</accession>
    <name>F6BAI5_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_1463</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96998.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1463"/>
    <dbReference type="EnsemblBacteria" id="AEF96998">
        <property type="protein sequence ID" value="AEF96998"/>
        <property type="gene ID" value="Metig_1463"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_1463"/>
    <dbReference type="HOGENOM" id="CLU_197251_0_0_2"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF96998.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="61" mass="6338" checksum="382D0EF3CCB16E52" modified="2011-07-27" version="1">MEIGILVAAAVAVAAIAAYYYVTNVKTSAKGAGDTANKTIIKINKTVYSEVEGLNDTSTGW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-06-02" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAN2</accession>
    <name>F6BAN2_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_0290</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95846.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798455.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF95846">
        <property type="protein sequence ID" value="AEF95846"/>
        <property type="gene ID" value="Metig_0290"/>
    </dbReference>
    <dbReference type="GeneID" id="10643125"/>
    <dbReference type="KEGG" id="mig:Metig_0290"/>
    <dbReference type="HOGENOM" id="CLU_862240_0_0_2"/>
    <dbReference type="OMA" id="YIIANIT"/>
    <dbReference type="OrthoDB" id="96164at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF95846.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="322" mass="37087" checksum="35FCDDE1B9E5ED79" modified="2011-07-27" version="1">MSNIKKIALVTIFLLFFCGCVETLSVKEVVNNSPYTHFVYLSKDAINEFIKENPNIIKEKGIPEDLLSIITSFMTGIYINKDDQSQIAIKTPLWMLGKLYQNENIIASNGYILINTKTINKPNPKFRDYLPNYNDIVSYDEDRAMSIKIDAYTHIGLVTKKDEYLKKILKGLKSIGKIKVINTSVIEKDGYIIANITCEPISLDINYLKENINLDLKPPITVNVVGWEKINENEYKKGLHFIKVYILPVNKETMKELINEQITNEMGNIEKVKDSKYKDWNIEEYMKNNIKTYIGYKEVEYGTVCIITDSLNNLDDVNVHLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAP5</accession>
    <name>F6BAP5_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">3-phosphoshikimate 1-carboxyvinyltransferase</fullName>
            <ecNumber evidence="7">2.5.1.19</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">5-enolpyruvylshikimate-3-phosphate synthase</fullName>
            <shortName evidence="7">EPSP synthase</shortName>
            <shortName evidence="7">EPSPS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">aroA</name>
        <name evidence="9" type="ordered locus">Metig_0303</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the transfer of the enolpyruvyl moiety of phosphoenolpyruvate (PEP) to the 5-hydroxyl of shikimate-3-phosphate (S3P) to produce enolpyruvyl shikimate-3-phosphate and inorganic phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>3-phosphoshikimate + phosphoenolpyruvate = 5-O-(1-carboxyvinyl)-3-phosphoshikimate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:21256"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57701"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="ChEBI" id="CHEBI:145989"/>
            <dbReference type="EC" id="2.5.1.19"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 6/7.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 7">Belongs to the EPSP synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.19" evidence="7"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95859.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798468.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0303"/>
    <dbReference type="EnsemblBacteria" id="AEF95859">
        <property type="protein sequence ID" value="AEF95859"/>
        <property type="gene ID" value="Metig_0303"/>
    </dbReference>
    <dbReference type="GeneID" id="10643138"/>
    <dbReference type="KEGG" id="mig:Metig_0303"/>
    <dbReference type="HOGENOM" id="CLU_024321_0_0_2"/>
    <dbReference type="OMA" id="YEDHRMA"/>
    <dbReference type="OrthoDB" id="80060at2157"/>
    <dbReference type="UniPathway" id="UPA00053">
        <property type="reaction ID" value="UER00089"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003866">
        <property type="term" value="F:3-phosphoshikimate 1-carboxyvinyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009073">
        <property type="term" value="P:aromatic amino acid family biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:cellular amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009423">
        <property type="term" value="P:chorismate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd01556">
        <property type="entry name" value="EPSP_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.65.10.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00210">
        <property type="entry name" value="EPSP_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001986">
        <property type="entry name" value="Enolpyruvate_Tfrase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036968">
        <property type="entry name" value="Enolpyruvate_Tfrase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006264">
        <property type="entry name" value="EPSP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023193">
        <property type="entry name" value="EPSP_synthase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013792">
        <property type="entry name" value="RNA3'P_cycl/enolpyr_Trfase_a/b"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00275">
        <property type="entry name" value="EPSP_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000505">
        <property type="entry name" value="EPSPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55205">
        <property type="entry name" value="SSF55205"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01356">
        <property type="entry name" value="aroA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00104">
        <property type="entry name" value="EPSP_SYNTHASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00885">
        <property type="entry name" value="EPSP_SYNTHASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="6 7" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="EPSP_synthase" evidence="8">
        <location>
            <begin position="5"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="region of interest" description="Shikimate-3-phosphate binding" evidence="7">
        <location>
            <begin position="20"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Phosphoenolpyruvate" evidence="7">
        <location>
            <begin position="87"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Shikimate-3-phosphate binding" evidence="7">
        <location>
            <begin position="164"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="7">
        <location>
            <position position="307"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="7">
        <location>
            <position position="335"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="25"/>
        </location>
        <ligand>
            <name>3-phosphoshikimate</name>
            <dbReference type="ChEBI" id="CHEBI:145989"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="118"/>
        </location>
        <ligand>
            <name>phosphoenolpyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="192"/>
        </location>
        <ligand>
            <name>3-phosphoshikimate</name>
            <dbReference type="ChEBI" id="CHEBI:145989"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="334"/>
        </location>
        <ligand>
            <name>3-phosphoshikimate</name>
            <dbReference type="ChEBI" id="CHEBI:145989"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="338"/>
        </location>
        <ligand>
            <name>phosphoenolpyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="380"/>
        </location>
        <ligand>
            <name>phosphoenolpyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001901"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AEF95859.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="425" mass="46340" checksum="779A23B9D110C3AC" modified="2011-07-27" version="1">MLIVRKTDRIEGVVNAPPSKSYTHRAVIGASLAEGTSRIKNPLNGADCLSSVHGCRMLGAEIELNEEYWIVKGTSPQTPDNIIDIGNSGTTLRILTGISSQIPKGYAILTGDDSIRRRPMQPLLDALKQLGIEAFSSKGNGTAPIIVKGGKLKGDVVKIRGDISSQFITSLMMTLPFNENDTEIVLTSPLKSRPYLEITMDVLDKFGIKIDETEDGFLVYGNQKYKAIDYTVEGDYSSASYLVAAGVLFNSDITINNLFKDSKQGDKAIIKIVKEMGADVKVKDDKIIIRGEYNLHGIDVDVKDIPDLVPTIAVLGCFAEGKTTIYNGEHVRLKECDRLSACAKELSKMGAKITEKPDGLVIEGVKKLKGAKLNTYHDHRLVMAFTIAGMKAEGETVIEGEEAVRISFPNFVEVMKSLGANIEVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAS4</accession>
    <name>F6BAS4_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DNA double-strand break repair Rad50 ATPase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">rad50</name>
        <name evidence="8" type="ordered locus">Metig_0332</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Part of the Rad50/Mre11 complex, which is involved in the early steps of DNA double-strand break (DSB) repair. The complex may facilitate opening of the processed DNA ends to aid in the recruitment of HerA and NurA. Rad50 controls the balance between DNA end bridging and DNA resection via ATP-dependent structural rearrangements of the Rad50/Mre11 complex.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="5">Binds 1 zinc ion per homodimer.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer. Forms a heterotetramer composed of two Mre11 subunits and two Rad50 subunits.</text>
    </comment>
    <comment type="domain">
        <text evidence="5">The two conserved Cys that bind zinc constitute the zinc-hook, which separates the large intramolecular coiled coil regions. The 2 Cys residues coordinate one molecule of zinc with the help of the 2 Cys residues of the zinc-hook of another Rad50 molecule, thereby forming a V-shaped homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the SMC family. RAD50 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95888.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798497.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0332"/>
    <dbReference type="EnsemblBacteria" id="AEF95888">
        <property type="protein sequence ID" value="AEF95888"/>
        <property type="gene ID" value="Metig_0332"/>
    </dbReference>
    <dbReference type="GeneID" id="10643169"/>
    <dbReference type="KEGG" id="mig:Metig_0332"/>
    <dbReference type="HOGENOM" id="CLU_004785_0_2_2"/>
    <dbReference type="OMA" id="FELPYSH"/>
    <dbReference type="OrthoDB" id="1771at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006302">
        <property type="term" value="P:double-strand break repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00449">
        <property type="entry name" value="RAD50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038729">
        <property type="entry name" value="Rad50/SbcC_AAA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022982">
        <property type="entry name" value="Rad50_ATPase_archaeal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013134">
        <property type="entry name" value="Zn_hook_RAD50"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13476">
        <property type="entry name" value="AAA_23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04423">
        <property type="entry name" value="Rad50_zn_hook"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51131">
        <property type="entry name" value="ZN_HOOK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0227">DNA damage</keyword>
    <keyword evidence="5" id="KW-0234">DNA repair</keyword>
    <keyword evidence="3 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 5 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Zinc-hook" evidence="7">
        <location>
            <begin position="449"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="164"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="648"/>
            <end position="841"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="12"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="32"/>
            <end position="38"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="126"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5 6">
        <location>
            <position position="494"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5 6">
        <location>
            <position position="497"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEF95888.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="999" mass="117907" checksum="22F3B94D37B1882B" modified="2011-07-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-06-02" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAV5</accession>
    <name>F6BAV5_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_1508</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97042.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799636.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF97042">
        <property type="protein sequence ID" value="AEF97042"/>
        <property type="gene ID" value="Metig_1508"/>
    </dbReference>
    <dbReference type="GeneID" id="10644381"/>
    <dbReference type="KEGG" id="mig:Metig_1508"/>
    <dbReference type="HOGENOM" id="CLU_2645917_0_0_2"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF97042.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="76" mass="8936" checksum="0976B04FE3C41A96" modified="2011-07-27" version="1">MVVKDEDIDSIICKCIDALKKCENDFVMYILRLLNQLSPSKKYAEDVINVLDKFVDNYDFEDIISNILEKYNNIIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2020-08-12" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAX2</accession>
    <name>F6BAX2_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_1525</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97059.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF97059">
        <property type="protein sequence ID" value="AEF97059"/>
        <property type="gene ID" value="Metig_1525"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_1525"/>
    <dbReference type="HOGENOM" id="CLU_1700288_0_0_2"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF97059.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="154" mass="18133" checksum="D29AFCD69677B733" modified="2011-07-27" version="1">MLKKMGSIVYMLFILLSEVNSCVELSKTLCIFGIYFWNRYAPLVCLSKNGKAVYYITNNLFMDFEEFQEVRNASWRIEEFHRGVKQCCNIGNFFVRKRLPILGHISLAMRAFFILERVRIDKKITWYEFRRELNRIAVGNAIISLCKEAGLLLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAZ9</accession>
    <name>F6BAZ9_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0305 protein Metig_1552</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_1552</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0305 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97086.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799680.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1552"/>
    <dbReference type="EnsemblBacteria" id="AEF97086">
        <property type="protein sequence ID" value="AEF97086"/>
        <property type="gene ID" value="Metig_1552"/>
    </dbReference>
    <dbReference type="GeneID" id="10644425"/>
    <dbReference type="KEGG" id="mig:Metig_1552"/>
    <dbReference type="HOGENOM" id="CLU_089549_1_0_2"/>
    <dbReference type="OMA" id="YYCPVKN"/>
    <dbReference type="OrthoDB" id="104709at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00763">
        <property type="entry name" value="UPF0305"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019215">
        <property type="entry name" value="DUF2115"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09888">
        <property type="entry name" value="DUF2115"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004959">
        <property type="entry name" value="UCP004959"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="70"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF97086.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="167" mass="19930" checksum="F9DF52290D19FB7A" modified="2011-07-27" version="1">MNSRELFKKLKKEAKEFSIYDMMKARCFMENDAKYLPPQYRNEFLESLGMYFYNVLNEIKNRDKNSVESFEIDEEKLEKLIERIESFKKMNSENEDAFIRLSKIIAPYLIFIAKKPIHPENLVFPGKKKIIKKGNTYYCPVKNKQKNEYSLCEFCVCKDISELDSDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F6BB02</accession>
    <name>F6BB02_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">PP-loop domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1555</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97089.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799683.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1555"/>
    <dbReference type="EnsemblBacteria" id="AEF97089">
        <property type="protein sequence ID" value="AEF97089"/>
        <property type="gene ID" value="Metig_1555"/>
    </dbReference>
    <dbReference type="GeneID" id="10644428"/>
    <dbReference type="KEGG" id="mig:Metig_1555"/>
    <dbReference type="HOGENOM" id="CLU_1014190_0_0_2"/>
    <dbReference type="OMA" id="GYTTNCL"/>
    <dbReference type="OrthoDB" id="25986at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012121">
        <property type="entry name" value="ATPase_PP-loop_MJ1599"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002500">
        <property type="entry name" value="PAPS_reduct"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01507">
        <property type="entry name" value="PAPS_reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036668">
        <property type="entry name" value="ATPase_UCP036668"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PAPS_reduct" evidence="1">
        <location>
            <begin position="62"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF97089.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="287" mass="33735" checksum="72489288704ADB0B" modified="2011-07-27" version="1">MEEVKCEMCLHTSKTRKIYDYEGKKLCKECIVTLKYPRDFEKMKKEVEEFLSKKRINKRYHCIVALSGGKDSVVALYLAKEKFNLNPLCVTVDNKYLAKEAIENCYNVTRHLGVDWIVLQRDYTKLFEEALNKGESPCRKCSEWNMREIWRIAKLMDIDVIITGHELPFGTTAIREMKEGIKMVRLLAPYRLTEEERYNILKKLPWKKPNLGGYTTNCLVLGVALERFYKKYGFSFEFDRISAMVRFGLISREKAMKALECPKVPKEVYEELKRRGLKINFEGDDES</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>F6BB34</accession>
    <name>F6BB34_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 3">Triosephosphate isomerase</fullName>
            <shortName evidence="2">TIM</shortName>
            <shortName evidence="2">TPI</shortName>
            <ecNumber evidence="2 3">5.3.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">Triose-phosphate isomerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2" type="primary">tpiA</name>
        <name evidence="4" type="ordered locus">Metig_0363</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Involved in the gluconeogenesis. Catalyzes stereospecifically the conversion of dihydroxyacetone phosphate (DHAP) to D-glyceraldehyde-3-phosphate (G3P).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 3">
            <text>D-glyceraldehyde 3-phosphate = dihydroxyacetone phosphate</text>
            <dbReference type="Rhea" id="RHEA:18585"/>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="5.3.1.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 3">Carbohydrate biosynthesis; gluconeogenesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 3">Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate from glycerone phosphate: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homotetramer; dimer of dimers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 3">Belongs to the triosephosphate isomerase family.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.1" evidence="2 3"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95919.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798528.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0363"/>
    <dbReference type="EnsemblBacteria" id="AEF95919">
        <property type="protein sequence ID" value="AEF95919"/>
        <property type="gene ID" value="Metig_0363"/>
    </dbReference>
    <dbReference type="GeneID" id="10643200"/>
    <dbReference type="KEGG" id="mig:Metig_0363"/>
    <dbReference type="HOGENOM" id="CLU_104921_0_0_2"/>
    <dbReference type="OMA" id="IPVYAQH"/>
    <dbReference type="OrthoDB" id="60670at2157"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00189"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00138"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004807">
        <property type="term" value="F:triose-phosphate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00311">
        <property type="entry name" value="TIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00147_A">
        <property type="entry name" value="TIM_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035990">
        <property type="entry name" value="TIM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000652">
        <property type="entry name" value="Triosephosphate_isomerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022891">
        <property type="entry name" value="Triosephosphate_isomerase_arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21139">
        <property type="entry name" value="PTHR21139"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00121">
        <property type="entry name" value="TIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51351">
        <property type="entry name" value="SSF51351"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00419">
        <property type="entry name" value="tim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51440">
        <property type="entry name" value="TIM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 3" id="KW-0312">Gluconeogenesis</keyword>
    <keyword evidence="2 3" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="1 2 3" id="KW-0413">Isomerase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Substrate binding" evidence="2">
        <location>
            <begin position="8"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="2">
        <location>
            <begin position="201"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="active site" description="Electrophile" evidence="2">
        <location>
            <position position="92"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="2">
        <location>
            <position position="140"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="145"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="180"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU363013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEF95919.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="221" mass="23466" checksum="1A80F30292720B05" modified="2011-07-27" version="1">MKPVIIINYKTYKESIGKRGLEIAKIAEKVSEESGITIGVAPQFVDLRIVAENVNIPVYAQHIDAITPGSHTGHVLAEAIKDCGVKGTLLNHSERNMLLSDIDKAIEIAKSLELETIVCTNNINVSKAVAALNPTYIAIEPPELIGTGIPVSKANPEIVEGTVKEVRKINKDVKILCGAGISTGSDVKAALELGTDGVLLASGVVKAKDVEKAIRDLISEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>F6BB38</accession>
    <name>F6BB38_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Metal dependent phosphohydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0367</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95923.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798532.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0367"/>
    <dbReference type="EnsemblBacteria" id="AEF95923">
        <property type="protein sequence ID" value="AEF95923"/>
        <property type="gene ID" value="Metig_0367"/>
    </dbReference>
    <dbReference type="GeneID" id="10643204"/>
    <dbReference type="KEGG" id="mig:Metig_0367"/>
    <dbReference type="HOGENOM" id="CLU_026821_3_1_2"/>
    <dbReference type="OMA" id="RRQTPRW"/>
    <dbReference type="OrthoDB" id="11161at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045509">
        <property type="entry name" value="HD_assoc_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006674">
        <property type="entry name" value="HD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006675">
        <property type="entry name" value="HDIG_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01966">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19276">
        <property type="entry name" value="HD_assoc_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00471">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00277">
        <property type="entry name" value="HDIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51831">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HD" evidence="1">
        <location>
            <begin position="56"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF95923.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="463" mass="53981" checksum="E28F586C41D80056" modified="2011-07-27" version="1">MIVSNNKKVIRDPIHKDIPLSHEEIKLVDTIDLQRLRNIKQTGLTYLVYPSANHTRFEHSLGTMYIAGEIAEKLNADVELTRITALLHDIGHPPFSHTLEICGYDHEQVAKAKIKKMEFENYSHKEIIDVLNKKGLEGKIISGDVDADRMDYLLRDSYHTGVAYGLIDLPRIMRSIVTYEEMGKLRIGILEKGIHAVESLLIARHQMYPTVYMHPTSRIADTMLKRAVMYALEDKLFGINDLAYMDDIDLIYTLRNSEGEENKLMKMIDERRLFKKIITYKYTDLTPRERWVLISMDEEDVRNLEKELSEYLGTSVFLDIPDYPKMEEHSVTIIIDDKRYRLDEVSPLAKSLKPSEIRLWDVGVYAPPDKLKEIKEKLEICKRDLLKEFIKDIPVKSTLLEIIEEYEIIKGRGTFLSIAKEHGMTESEFFSELHKLVFCGLIKEKIEKIGGIYRYDYSRLRYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-09-29" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>F6BB50</accession>
    <name>F6BB50_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_0379</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95935.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798544.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF95935">
        <property type="protein sequence ID" value="AEF95935"/>
        <property type="gene ID" value="Metig_0379"/>
    </dbReference>
    <dbReference type="GeneID" id="10643216"/>
    <dbReference type="KEGG" id="mig:Metig_0379"/>
    <dbReference type="HOGENOM" id="CLU_081493_2_0_2"/>
    <dbReference type="OMA" id="DFNILAY"/>
    <dbReference type="OrthoDB" id="86918at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF95935.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="155" mass="17596" checksum="5990F4135B38A98C" modified="2011-07-27" version="1">MGGEDAVIIPLKLLLHKYGKEKVKNLLNTFKCSINTDVEHFLKNSSILFEEINKSRTYLVIKKGTTDILGYFTLTLSILKIVEDVSKKTLKKLDGILKDKTEFPVYLIGQLGKNEMFWNEITGSYLLESAISIIYDAYEIVGGRIVLVETLNNFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>F6BB71</accession>
    <name>F6BB71_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 4">UTP--glucose-1-phosphate uridylyltransferase</fullName>
            <ecNumber evidence="3 4">2.7.7.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">UDP-glucose pyrophosphorylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Metig_0400</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 4">
            <text>alpha-D-glucose 1-phosphate + H(+) + UTP = diphosphate + UDP-alpha-D-glucose</text>
            <dbReference type="Rhea" id="RHEA:19889"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
            <dbReference type="ChEBI" id="CHEBI:58601"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="EC" id="2.7.7.9"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the UDPGP type 2 family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.9" evidence="3 4"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95956.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798565.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0400"/>
    <dbReference type="EnsemblBacteria" id="AEF95956">
        <property type="protein sequence ID" value="AEF95956"/>
        <property type="gene ID" value="Metig_0400"/>
    </dbReference>
    <dbReference type="GeneID" id="10643237"/>
    <dbReference type="KEGG" id="mig:Metig_0400"/>
    <dbReference type="HOGENOM" id="CLU_029499_1_0_2"/>
    <dbReference type="OMA" id="MHYVRQG"/>
    <dbReference type="OrthoDB" id="78828at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003983">
        <property type="term" value="F:UTP:glucose-1-phosphate uridylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009058">
        <property type="term" value="P:biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006011">
        <property type="term" value="P:UDP-glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02541">
        <property type="entry name" value="UGPase_prokaryotic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005771">
        <property type="entry name" value="GalU_uridylyltTrfase_bac/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005835">
        <property type="entry name" value="NTP_transferase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43197">
        <property type="entry name" value="PTHR43197"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00483">
        <property type="entry name" value="NTP_transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01099">
        <property type="entry name" value="galU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="NTP_transferase" evidence="5">
        <location>
            <begin position="4"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU361259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEF95956.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="283" mass="31757" checksum="954A7EADFA85AC90" modified="2011-07-27" version="1">MIKKAIVPVAGFGTRLLPITKAQPKEMLPVVGKPIIQYVVEHLIDAGIKNILMVTGKGKQAIVNHFDKNFELEERLRRDGKISLLNTVREIDNLANIFYVRQKEQKGLGDAILCGEGFVGDEYFIAMVGDTIYSENVVKDLIKVHEKYGCSVIALERVPMEEVYKYGVIKGEEIEDGIYKITDMVEKPPVDKAPSNLIITGAYLLSPKIFECLKRTPPGRGGEIQITDAMKLLLKEEEILGVEIKCRRYDIGDLLGWLKANVELGIENVDGFKEYLKDFTGKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2020-06-17" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>F6BB89</accession>
    <name>F6BB89_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_0418</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95974.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF95974">
        <property type="protein sequence ID" value="AEF95974"/>
        <property type="gene ID" value="Metig_0418"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0418"/>
    <dbReference type="HOGENOM" id="CLU_2985686_0_0_2"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF95974.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="57" mass="6754" checksum="BA8F94E84DB9AD9B" modified="2011-07-27" version="1">MFMSFRYIYIELYKDKKIDGWSLGSSRSEATRTIVVCVVVWKYAADEFLTAVSRFKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBC5</accession>
    <name>F6BBC5_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glycosyl transferase family 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1599</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97132.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1599"/>
    <dbReference type="EnsemblBacteria" id="AEF97132">
        <property type="protein sequence ID" value="AEF97132"/>
        <property type="gene ID" value="Metig_1599"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_1599"/>
    <dbReference type="HOGENOM" id="CLU_025996_22_6_2"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glyco_trans_2-like" evidence="1">
        <location>
            <begin position="8"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF97132.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="67" mass="7628" checksum="3A6DC5DC6A8236CD" modified="2011-07-27" version="1">MSLDNLVSVIIPTYNSEKTIGICLESIKNQTYKNIEIIVVDKFSNDNTVEIAKKYTDKVFVINAKEM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-06-02" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBC8</accession>
    <name>F6BBC8_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1602</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97135.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799728.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF97135">
        <property type="protein sequence ID" value="AEF97135"/>
        <property type="gene ID" value="Metig_1602"/>
    </dbReference>
    <dbReference type="GeneID" id="10644475"/>
    <dbReference type="KEGG" id="mig:Metig_1602"/>
    <dbReference type="HOGENOM" id="CLU_791343_0_0_2"/>
    <dbReference type="OrthoDB" id="137230at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF97135.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="350" mass="40251" checksum="1F046F01ECFE81F4" modified="2011-07-27" version="1">MKIKKRLNPKILNAIILGCCGIALIASALYYSNQNLKVEIELFSANDTHEIYRYDELTIRLKNLESFTINPIFFINNGVDKKSWDIVTGPKSLKSGEEAIYVLRAPDADVAIEANKSFYIEVRDLSTGFSSFSQHYKLSSLRFPGIQNPSFYYWTYDPTKNIHKPFAWDATWWKIEKGEVAVLEKKENAVYLAVSNLTRKSGSWLMSGVQQAVDFPKKLRIVVKPMFSTPISKYPPYAAGIELADGNKRVWIVFTNETDEPILLKRYGELTYALYFVPTKIGKWNNITVNVESIYKEMGWNLPPKILVKREGNLLKIREVNLLAFVACYPGSECKKFEVYYKLIKCEERL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBC9</accession>
    <name>F6BBC9_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PTPS_related domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_1603</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97136.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799729.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1603"/>
    <dbReference type="EnsemblBacteria" id="AEF97136">
        <property type="protein sequence ID" value="AEF97136"/>
        <property type="gene ID" value="Metig_1603"/>
    </dbReference>
    <dbReference type="GeneID" id="10644476"/>
    <dbReference type="KEGG" id="mig:Metig_1603"/>
    <dbReference type="HOGENOM" id="CLU_501191_0_0_2"/>
    <dbReference type="OMA" id="WEYTRIL"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018776">
        <property type="entry name" value="Membrane_prot_PTPS-rel_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10131">
        <property type="entry name" value="PTPS_related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="107"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="158"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="185"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="207"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="230"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="292"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="323"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="356"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="387"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="domain" description="PTPS_related" evidence="2">
        <location>
            <begin position="84"/>
            <end position="475"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF97136.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="543" mass="63373" checksum="61C954DA2F1DF8A3" modified="2011-07-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBH6</accession>
    <name>F6BBH6_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">UDP-glucose 4-epimerase</fullName>
            <ecNumber evidence="2">5.1.3.2</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1651</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EC" id="5.1.3.2" evidence="2"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97183.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799774.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1651"/>
    <dbReference type="EnsemblBacteria" id="AEF97183">
        <property type="protein sequence ID" value="AEF97183"/>
        <property type="gene ID" value="Metig_1651"/>
    </dbReference>
    <dbReference type="GeneID" id="10644524"/>
    <dbReference type="KEGG" id="mig:Metig_1651"/>
    <dbReference type="HOGENOM" id="CLU_007383_1_7_2"/>
    <dbReference type="OMA" id="GEHLICN"/>
    <dbReference type="OrthoDB" id="43299at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003978">
        <property type="term" value="F:UDP-glucose 4-epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0413">Isomerase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Epimerase" evidence="1">
        <location>
            <begin position="3"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF97183.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="305" mass="34676" checksum="3B4CD7783DD7820D" modified="2011-07-27" version="1">MKILVTGGAGFIGSHIVDELIKNGHEVVILDNLVTGNKNNINPKAEFVEGDITNKNLDEKIDFQDIDVVIHQAAQINVRKSVEDPIYDGDVNVLGTLNILENMRRYDVKKIIYASSGGAVYGEPEYLPVDENHPIAPLSPYGLSKYCGEEYIKLYNRLYGIEYCILRYSNVYGERQDPRGEAGVISIFIDRILNNKNPIIFGDGNQTRDFVYVKDVARANLMALDWKNEIVNIGTGKETSVNELFKIIADELNYEDKPIYDKPREGEVRRIYLDIKKAEMLGWKPEVDLKEGIKRVVNWMKQMQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBJ4</accession>
    <name>F6BBJ4_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="3">CBS domain containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_0447</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96003.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798612.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96003">
        <property type="protein sequence ID" value="AEF96003"/>
        <property type="gene ID" value="Metig_0447"/>
    </dbReference>
    <dbReference type="GeneID" id="10643284"/>
    <dbReference type="KEGG" id="mig:Metig_0447"/>
    <dbReference type="HOGENOM" id="CLU_040681_9_3_2"/>
    <dbReference type="OMA" id="GIHHLYV"/>
    <dbReference type="OrthoDB" id="104239at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="SSF54631"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0129">CBS domain</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBS" evidence="2">
        <location>
            <begin position="11"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="2">
        <location>
            <begin position="73"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF96003.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="132" mass="14922" checksum="B1A1CD6E19A73A76" modified="2011-07-27" version="1">MLSKYLVRDIMKRGIVEVSLNDKISDIVKKMAENDISSVVVSDNQAFWGIITDTDILKHYHENLDTLKAEDIMNSKIIAISPEAPLEKAVEVMIENNIHHLYVRSELDDKIVGVISSKDIVKLMGSVQREQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBL3</accession>
    <name>F6BBL3_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CGGC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0466</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96022.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798630.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96022">
        <property type="protein sequence ID" value="AEF96022"/>
        <property type="gene ID" value="Metig_0466"/>
    </dbReference>
    <dbReference type="GeneID" id="10643303"/>
    <dbReference type="KEGG" id="mig:Metig_0466"/>
    <dbReference type="HOGENOM" id="CLU_147304_3_0_2"/>
    <dbReference type="OMA" id="SCFKAIN"/>
    <dbReference type="OrthoDB" id="91651at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014925">
        <property type="entry name" value="CGGC_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08821">
        <property type="entry name" value="CGGC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01078">
        <property type="entry name" value="CGGC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CGGC" evidence="1">
        <location>
            <begin position="2"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM01078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96022.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="128" mass="14126" checksum="9EF36782472090CF" modified="2011-07-27" version="1">MKVAIIACQKMVEMGCPGKEACVSCFKAINEKSGAFERYKDVELVAFTTCGGCPGKRFPMRIKMLKNACNAEAIHIANCSLLEPKCPYINFEEIAKKLMEELEVPIVFGTHTLVKKGEVVCTCGDKKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBL6</accession>
    <name>F6BBL6_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">4Fe-4S ferredoxin iron-sulfur binding domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0469</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96025.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0469"/>
    <dbReference type="EnsemblBacteria" id="AEF96025">
        <property type="protein sequence ID" value="AEF96025"/>
        <property type="gene ID" value="Metig_0469"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0469"/>
    <dbReference type="HOGENOM" id="CLU_050974_0_0_2"/>
    <dbReference type="OMA" id="VYCKVCE"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043256">
        <property type="entry name" value="MvhB-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00037">
        <property type="entry name" value="Fer4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12838">
        <property type="entry name" value="Fer4_7"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005658">
        <property type="entry name" value="FwdF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="21"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="61"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="105"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="144"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="184"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="232"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="264"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="301"/>
            <end position="330"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96025.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="352" mass="38231" checksum="FAA175BF2B43D974" modified="2011-07-27" version="1">MQIKEEYNGTITISREGVEKRVLTWSDHICVGCGICHDICPTKAITMGPLGAIAKGDIDAPKLDIDGNVCVLCGMCACACPFNAIDLKINDKSIKELPQYPKILRNITLSQETCVLCGQCEIVCPQGAIEVERELPPRKNLVLGEISINKDKCVLCGICAEYCPADAIDLIPNDMNSLSPKPIKDIVVDTDACVFCKVCEKACPHDAIEVICYKCPLAKRIEKPKLYTDIKGKTTIDKDLCVTCGWCENICPVNAIKVEKPFEGELIIDEKACNACGACVAICPCNALTFPKPADKAQKVPRLAVNQDLCVLCGACAKSCPVSAITVKRTKINMTETKATAWKKAFEKLLAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBL7</accession>
    <name>F6BBL7_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">4Fe-4S ferredoxin iron-sulfur binding domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0470</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96026.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798634.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0470"/>
    <dbReference type="EnsemblBacteria" id="AEF96026">
        <property type="protein sequence ID" value="AEF96026"/>
        <property type="gene ID" value="Metig_0470"/>
    </dbReference>
    <dbReference type="GeneID" id="10643307"/>
    <dbReference type="KEGG" id="mig:Metig_0470"/>
    <dbReference type="HOGENOM" id="CLU_139698_5_4_2"/>
    <dbReference type="OMA" id="WGGKGPY"/>
    <dbReference type="OrthoDB" id="118147at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13187">
        <property type="entry name" value="Fer4_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="2"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="48"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96026.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="79" mass="8280" checksum="AFB32FA9BFE42D82" modified="2011-07-27" version="1">MYKLVVYPEKCHGCGNCVVSCPVNAKDPNTWGGKGPETDEVVIRVENGVVTIVNGDLCGGCGACIEACPVDAIKLVIEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBP1</accession>
    <name>F6BBP1_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_0494</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96050.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0494"/>
    <dbReference type="EnsemblBacteria" id="AEF96050">
        <property type="protein sequence ID" value="AEF96050"/>
        <property type="gene ID" value="Metig_0494"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0494"/>
    <dbReference type="HOGENOM" id="CLU_168724_0_0_2"/>
    <dbReference type="OMA" id="LEHEVHP"/>
    <dbReference type="OrthoDB" id="120328at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF96050.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="109" mass="12405" checksum="E11822736837E7D2" modified="2011-07-27" version="1">MYVHVCPYCLNPVLCYQKDYLEHEVHPVKVDVENLECSVLLGSKIYKNAQCSNLQGLKIVAGKIASKLKLGGDEKKAFMEKVEELKRQHPRWKDYKILETALNSVLQGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBP5</accession>
    <name>F6BBP5_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0498</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96054.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798662.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0498"/>
    <dbReference type="EnsemblBacteria" id="AEF96054">
        <property type="protein sequence ID" value="AEF96054"/>
        <property type="gene ID" value="Metig_0498"/>
    </dbReference>
    <dbReference type="GeneID" id="10643335"/>
    <dbReference type="KEGG" id="mig:Metig_0498"/>
    <dbReference type="HOGENOM" id="CLU_2177905_0_0_2"/>
    <dbReference type="OMA" id="SMVLKPQ"/>
    <dbReference type="OrthoDB" id="118183at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="29"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="56"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="82"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96054.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="107" mass="11598" checksum="9F68409106DEB802" modified="2011-07-27" version="1">MIQQVIAAILTFLLCFVCSDKVMVIGEKLGLDGTLAWCVTTFLVIISVWKLASRDWGFGDAVATIVISILIMLYYTQDVGKIVLEISKVILGISVGVIIGYLAKGDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBQ0</accession>
    <name>F6BBQ0_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_0503</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96059.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798667.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0503"/>
    <dbReference type="EnsemblBacteria" id="AEF96059">
        <property type="protein sequence ID" value="AEF96059"/>
        <property type="gene ID" value="Metig_0503"/>
    </dbReference>
    <dbReference type="GeneID" id="10643340"/>
    <dbReference type="KEGG" id="mig:Metig_0503"/>
    <dbReference type="HOGENOM" id="CLU_110993_0_0_2"/>
    <dbReference type="OMA" id="LETQNEG"/>
    <dbReference type="OrthoDB" id="121671at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="178"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="76"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF96059.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="207" mass="23871" checksum="9A8092FFBF25A499" modified="2011-07-27" version="1">MIQKIMTILMLVLLSGAYANSIVVYNPETGTYYTCYPKNTTNGKTIYILNVSSATRDDNITIIHKIVIENQTAVMVRAYNALIAELERNISKLQKELDKKNKLLAEYISYKKKNVELQKNITQLQKQITTLKAENELIKQQNEVYKSIITDLINKQSNETKEEYIMAYNEWKKDVSNFKTSVIIGGLVCLIIGVLLIKFKKRYDYPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBS6</accession>
    <name>F6BBS6_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="3">AAA ATPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_1674</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the archaeal ATPase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97206.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799797.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF97206">
        <property type="protein sequence ID" value="AEF97206"/>
        <property type="gene ID" value="Metig_1674"/>
    </dbReference>
    <dbReference type="GeneID" id="10644547"/>
    <dbReference type="KEGG" id="mig:Metig_1674"/>
    <dbReference type="HOGENOM" id="CLU_068608_0_0_2"/>
    <dbReference type="OMA" id="IRRWINI"/>
    <dbReference type="OrthoDB" id="47389at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011579">
        <property type="entry name" value="ATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01637">
        <property type="entry name" value="ATPase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA" evidence="2">
        <location>
            <begin position="21"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF97206.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="338" mass="39886" checksum="56AD3CAC0D3193DC" modified="2011-07-27" version="1">MKFYNREKELNYLKTYCQLEPNSILFVYGPKSSGKSTVMRRVIKELSEDIVFFYYNLRKYATPTKDEFLSIFFEKSDKKYLPNKLEFNLGVFKFGIEKNQDFKNLSLNDVFAKINESINAVIKEGKKPVLIIDELQKLKNIYFNGEKSLLNELFNLFVSLTKMEHLCHVICLTSDTLFIEEIYQNSTLKNASEYYLIDWLNRDCIKDILEEEGFNEEEIDYAINYLSLPYEVSQLINNKKLGLSVEETIRRWINIEKDRLVYLLKSNKERKENLLKVLSKFKEKIKVNIDDFEDEIFEDVKFLIKNEILFYDVVNGVIKPTSIIEWEAIKLGGGKNAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBT0</accession>
    <name>F6BBT0_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="3">DEAD/DEAH box helicase domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_1678</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97210.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799801.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1678"/>
    <dbReference type="EnsemblBacteria" id="AEF97210">
        <property type="protein sequence ID" value="AEF97210"/>
        <property type="gene ID" value="Metig_1678"/>
    </dbReference>
    <dbReference type="GeneID" id="10644551"/>
    <dbReference type="KEGG" id="mig:Metig_1678"/>
    <dbReference type="HOGENOM" id="CLU_010611_0_0_2"/>
    <dbReference type="OMA" id="SRRRCHE"/>
    <dbReference type="OrthoDB" id="3149at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043852">
        <property type="entry name" value="DUF5814"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19131">
        <property type="entry name" value="DUF5814"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0347">Helicase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="1">
        <location>
            <begin position="210"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="2">
        <location>
            <begin position="385"/>
            <end position="567"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF97210.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="801" mass="92953" checksum="FC63811F8B8D079E" modified="2011-07-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBT5</accession>
    <name>F6BBT5_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 7">Methionine aminopeptidase</fullName>
            <shortName evidence="6">MAP</shortName>
            <shortName evidence="6">MetAP</shortName>
            <ecNumber evidence="6 7">3.4.11.18</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Peptidase M</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">map</name>
        <name evidence="9" type="ordered locus">Metig_1683</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6 7">Removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 6 7">
            <text>Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides.</text>
            <dbReference type="EC" id="3.4.11.18"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6 7">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
        <cofactor evidence="6 7">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <cofactor evidence="6 7">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <cofactor evidence="6 7">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
        <text evidence="6 7">Binds 2 divalent metal cations per subunit. Has a high-affinity and a low affinity metal-binding site. The true nature of the physiological cofactor is under debate. The enzyme is active with cobalt, zinc, manganese or divalent iron ions. Most likely, methionine aminopeptidases function as mononuclear Fe(2+)-metalloproteases under physiological conditions, and the catalytically relevant metal-binding site has been assigned to the histidine-containing high-affinity site.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the peptidase M24A family. Methionine aminopeptidase archaeal type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.4.11.18" evidence="6 7"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97215.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799806.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1683"/>
    <dbReference type="EnsemblBacteria" id="AEF97215">
        <property type="protein sequence ID" value="AEF97215"/>
        <property type="gene ID" value="Metig_1683"/>
    </dbReference>
    <dbReference type="GeneID" id="10644556"/>
    <dbReference type="KEGG" id="mig:Metig_1683"/>
    <dbReference type="HOGENOM" id="CLU_015857_7_0_2"/>
    <dbReference type="OMA" id="NEIAAHY"/>
    <dbReference type="OrthoDB" id="82853at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070084">
        <property type="term" value="P:protein initiator methionine removal"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01088">
        <property type="entry name" value="MetAP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01975">
        <property type="entry name" value="MetAP_2_arc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036005">
        <property type="entry name" value="Creatinase/aminopeptidase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028595">
        <property type="entry name" value="MetAP_archaeal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000994">
        <property type="entry name" value="Pept_M24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001714">
        <property type="entry name" value="Pept_M24_MAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002468">
        <property type="entry name" value="Pept_M24A_MAP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018349">
        <property type="entry name" value="Pept_M24A_MAP2_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00557">
        <property type="entry name" value="Peptidase_M24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00599">
        <property type="entry name" value="MAPEPTIDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55920">
        <property type="entry name" value="SSF55920"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00501">
        <property type="entry name" value="met_pdase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01202">
        <property type="entry name" value="MAP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6 7" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="5 6 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 6 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 6 7" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase_M24" evidence="8">
        <location>
            <begin position="8"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="65"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="85"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="96"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="156"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="164"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="189"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AEF97215.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="295" mass="32871" checksum="2D3ADFB1208A0B60" modified="2011-07-27" version="1">MNEETYNKIIEAGKIVSKVREEAIKMIKPGVKLYDVAEFVENRIKELGGEPAFPCNISINNIAAHYTPCHNDDKIFGENDVVKLDLGAHVDGYIADTAITVDLSNSYNDLKKAAEDALETVIKEINPPMNVGEMGRIIEEVINSYGYKPISNLSGHVMYRYVLHSGISIPNVKERTNDVVDVGDLVAIEPFATDGFGMVKDGSVGHIYKFITSKPMRLPTARTLLKKIEKEYPYLPFAERWVVKENPKFKTALKSLIMAGCVYSYPTLVERNNGMVTQAEHTVLITENGVEVVTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBU5</accession>
    <name>F6BBU5_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Methanogenesis marker protein 7</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_1693</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97225.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799815.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1693"/>
    <dbReference type="EnsemblBacteria" id="AEF97225">
        <property type="protein sequence ID" value="AEF97225"/>
        <property type="gene ID" value="Metig_1693"/>
    </dbReference>
    <dbReference type="GeneID" id="10644566"/>
    <dbReference type="KEGG" id="mig:Metig_1693"/>
    <dbReference type="HOGENOM" id="CLU_890295_0_0_2"/>
    <dbReference type="OMA" id="AKPNMIG"/>
    <dbReference type="OrthoDB" id="33494at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026327">
        <property type="entry name" value="Me_CoM_Rdtase_prot-C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011312">
        <property type="entry name" value="UCP019164_menthan"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04609">
        <property type="entry name" value="MCR_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF019164">
        <property type="entry name" value="UCP019164"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03274">
        <property type="entry name" value="methan_mark_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF97225.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="303" mass="34660" checksum="DA361CB9BF9D4357" modified="2011-07-27" version="1">MYEIVRYEGGVYKNNILKEWIEDIGGFVIQEHVMQLDVYMTVALPQSEIEAFKEEAKKYKGKVVETPLAGIEIAVVAPSLSRHHLPHTACDISEYVRRFGAKPNMIGLAHGAGKSISRISEREKKLIEEHDIAIYVMGNFANCIKDKTHLFDVDIPVVVTGGPEELDIPYPYVGNLGRRAHRLRHGEEIRALRKMIDVVTELINERRKELSYDPPIVPPVVVKDEIEKRVEEVFNILSPMPIVTQLDGLRIKMDYDKYKDKIANVKVGNYVLKDIAYITRSMMKNYILVKIKPKSEVEFELGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBX2</accession>
    <name>F6BBX2_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Molybdenum cofactor synthesis domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metig_1720</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; molybdopterin biosynthesis.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97252.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799841.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1720"/>
    <dbReference type="EnsemblBacteria" id="AEF97252">
        <property type="protein sequence ID" value="AEF97252"/>
        <property type="gene ID" value="Metig_1720"/>
    </dbReference>
    <dbReference type="GeneID" id="10644594"/>
    <dbReference type="KEGG" id="mig:Metig_1720"/>
    <dbReference type="HOGENOM" id="CLU_010186_3_0_2"/>
    <dbReference type="OMA" id="IEAKTHS"/>
    <dbReference type="OrthoDB" id="51611at2157"/>
    <dbReference type="UniPathway" id="UPA00344"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006777">
        <property type="term" value="P:Mo-molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032324">
        <property type="term" value="P:molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00887">
        <property type="entry name" value="MoeA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.340.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.980.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036425">
        <property type="entry name" value="MoaB/Mog-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001453">
        <property type="entry name" value="MoaB/Mog_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008284">
        <property type="entry name" value="MoCF_biosynth_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038987">
        <property type="entry name" value="MoeA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005111">
        <property type="entry name" value="MoeA_C_domain_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036688">
        <property type="entry name" value="MoeA_C_domain_IV_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005110">
        <property type="entry name" value="MoeA_linker/N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036135">
        <property type="entry name" value="MoeA_linker/N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024370">
        <property type="entry name" value="PBP_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10192">
        <property type="entry name" value="PTHR10192"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00994">
        <property type="entry name" value="MoCF_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03454">
        <property type="entry name" value="MoeA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03453">
        <property type="entry name" value="MoeA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12727">
        <property type="entry name" value="PBP_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00852">
        <property type="entry name" value="MoCF_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53218">
        <property type="entry name" value="SSF53218"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63867">
        <property type="entry name" value="SSF63867"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63882">
        <property type="entry name" value="SSF63882"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00177">
        <property type="entry name" value="molyb_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01079">
        <property type="entry name" value="MOCF_BIOSYNTHESIS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0501">Molybdenum cofactor biosynthesis</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MoCF_biosynth" evidence="3">
        <location>
            <begin position="185"/>
            <end position="323"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEF97252.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="617" mass="68095" checksum="9D850B7F63B7C413" modified="2011-07-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBZ6</accession>
    <name>F6BBZ6_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Transcriptional regulator TrmB</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_0522</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96077.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798685.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96077">
        <property type="protein sequence ID" value="AEF96077"/>
        <property type="gene ID" value="Metig_0522"/>
    </dbReference>
    <dbReference type="GeneID" id="10643359"/>
    <dbReference type="KEGG" id="mig:Metig_0522"/>
    <dbReference type="HOGENOM" id="CLU_668379_0_0_2"/>
    <dbReference type="OMA" id="MENDFEL"/>
    <dbReference type="OrthoDB" id="62993at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF96077.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="410" mass="48104" checksum="01D80400FBB038A6" modified="2011-07-27" version="1">MNNKIKLNETDKKVLDFILENGESTRQEIIDALGVKSGSLTNSINKLEKLGYIKTEKQGNQTVIIPITPTQPSIHNDNTNIIHLDFSKIKSLTHLRDIINSHLRANNWSIGNYDLVITALIIAKFTQNLRLLIFGSQGIGKTCCIKAVFPDNDGFIEYDLHRKQLKEVVNLREHIRIIEQQYRHTWGAENPATFDQYTIVPLSRIAPSDLVFRFIPIRVLQYKYVPNYRPVVFDISNFKLIEPPLDVYDKLHNIMKHLLYFNNCDESAKSKIRDFKYREILSLYSLTAKEGEFNLNAEQWKIERKYERYLENPFRSVNLRVLQEDEEMWFRASLDMQLMRNSYEVLRFAFSISRSEDAIGETYDFIKEILATWTKVRDERPKRRDRSKVKVRSEAQSYYVDSQGVVHNVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>F6BC05</accession>
    <name>F6BC05_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_0531</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96086.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798694.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0531"/>
    <dbReference type="EnsemblBacteria" id="AEF96086">
        <property type="protein sequence ID" value="AEF96086"/>
        <property type="gene ID" value="Metig_0531"/>
    </dbReference>
    <dbReference type="GeneID" id="10643369"/>
    <dbReference type="KEGG" id="mig:Metig_0531"/>
    <dbReference type="HOGENOM" id="CLU_061502_0_0_2"/>
    <dbReference type="OMA" id="ICPFTTK"/>
    <dbReference type="OrthoDB" id="27038at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd07894">
        <property type="entry name" value="Adenylation_RNA_ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041596">
        <property type="entry name" value="Lig_Pab1020_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021122">
        <property type="entry name" value="RNA_ligase_dom_REL/Rnl2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001072">
        <property type="entry name" value="RNA_ligase_Pab1020"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18330">
        <property type="entry name" value="Lig_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09414">
        <property type="entry name" value="RNA_ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01048">
        <property type="entry name" value="Y414FAMILY"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01209">
        <property type="entry name" value="TIGR01209"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RNA_ligase" evidence="1">
        <location>
            <begin position="86"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="domain" description="Lig_C" evidence="2">
        <location>
            <begin position="257"/>
            <end position="384"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF09414"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF18330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF96086.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="386" mass="45241" checksum="A6C1B75154AAEB31" modified="2011-07-27" version="1">MEIDYALIAERLKLREEDIRRGFKRGIIIREEYKGVPMIAFRKKIKHIERGTALFLNENLDYIMGYPKIRRALVLNPTIKNYFIDKVVVEEKLDGYNVRIAKINNNIVAITRGGHVCPFTTKKAIQILNTKFFDNNPNLMLCGEMIGLNNPYVPHYYEEVDRGWENLGFYIFDIRDRETNEPLSIKEKIEICKKYNLPYVEPIGEFDKEDAHEYIKEIIDRLNAENREGVVLKDPDMAINPIKYTTHKTQCGDLKAAFTFFYDLGVDFMFSRVVREGYQAFEFNENDDALKKRALDIGESILYPMVDTIKKVHSGERVTEDFEIVVDSEEDLKEFLEYLKRLHMQFIIKNMEKIKADDEYKIKVKIGKIYSATNDKVLSHLNGGLW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>F6BC26</accession>
    <name>F6BC26_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">5-formaminoimidazole-4-carboxamide-1-(beta)-D-ribofuranosyl 5'-monophosphate synthetase</fullName>
            <ecNumber evidence="3">6.3.4.23</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">5-aminoimidazole-4-carboxamide-1-beta-D-ribofuranosyl 5'-monophosphate--formate ligase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">purP</name>
        <name evidence="6" type="ordered locus">Metig_0553</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the ATP- and formate-dependent formylation of 5-aminoimidazole-4-carboxamide-1-beta-d-ribofuranosyl 5'-monophosphate (AICAR) to 5-formaminoimidazole-4-carboxamide-1-beta-d-ribofuranosyl 5'-monophosphate (FAICAR) in the absence of folates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + ATP + formate = 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide + ADP + phosphate</text>
            <dbReference type="Rhea" id="RHEA:24836"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58467"/>
            <dbReference type="ChEBI" id="CHEBI:58475"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.4.23"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Purine metabolism; IMP biosynthesis via de novo pathway; 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide (formate route): step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the phosphohexose mutase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.23" evidence="3"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96107.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798714.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0553"/>
    <dbReference type="EnsemblBacteria" id="AEF96107">
        <property type="protein sequence ID" value="AEF96107"/>
        <property type="gene ID" value="Metig_0553"/>
    </dbReference>
    <dbReference type="GeneID" id="10643391"/>
    <dbReference type="KEGG" id="mig:Metig_0553"/>
    <dbReference type="HOGENOM" id="CLU_065084_0_0_2"/>
    <dbReference type="OMA" id="CIHYFYS"/>
    <dbReference type="OrthoDB" id="21169at2157"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00134"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016879">
        <property type="term" value="F:ligase activity, forming carbon-nitrogen bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01163">
        <property type="entry name" value="IMP_biosynth_PurP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023656">
        <property type="entry name" value="IMP_biosynth_PurP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009720">
        <property type="entry name" value="IMP_biosynth_PurP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010672">
        <property type="entry name" value="IMP_biosynth_PurP_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38147">
        <property type="entry name" value="PTHR38147"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06849">
        <property type="entry name" value="DUF1246"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06973">
        <property type="entry name" value="DUF1297"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004602">
        <property type="entry name" value="ATPgrasp_PurP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="SSF52440"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="3 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-grasp" evidence="5">
        <location>
            <begin position="116"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="27"/>
        </location>
        <ligand>
            <name>5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide</name>
            <dbReference type="ChEBI" id="CHEBI:58475"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="94"/>
        </location>
        <ligand>
            <name>5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide</name>
            <dbReference type="ChEBI" id="CHEBI:58475"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="230"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="258"/>
        </location>
        <ligand>
            <name>5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide</name>
            <dbReference type="ChEBI" id="CHEBI:58475"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEF96107.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="361" mass="40907" checksum="2DCE7D26BF13D8C4" modified="2011-07-27" version="1">MISREEMLEIFEGYNKDEITIATLGSHTALHILKGAKLEGFSTAVITVKGRDVPYKRFKVADKYIYIDNFADISKEEVQEKLREMNAIIVPHGSFIAYCGLDNVENNFRVPMFGNRKILRWEAERDLEGKLLRESGLRLPKKYNSPDEIDGPVMVKFPGARGGKGYFVCSSKEEFDAKVKQLKEKGILTDEDIEKAHIEEYVVGTNFCIHYFYSPLKDEVELMGMDRRYESNIDGLVRIPAKDQLEMDLNASYVITGNIPVVIRESLLPQVFEMGDKLVAKAKEIVPPGMIGPFCLQSLCNENLELVVFEMSARIDGGTNSFMNGSAYSFLYHGEPLSMGQRIAREIRMALELDMIDKIIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-06-02" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>F6BC27</accession>
    <name>F6BC27_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_0554</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96108.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798715.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96108">
        <property type="protein sequence ID" value="AEF96108"/>
        <property type="gene ID" value="Metig_0554"/>
    </dbReference>
    <dbReference type="GeneID" id="10643392"/>
    <dbReference type="KEGG" id="mig:Metig_0554"/>
    <dbReference type="HOGENOM" id="CLU_477877_0_0_2"/>
    <dbReference type="OMA" id="EYLCIWI"/>
    <dbReference type="OrthoDB" id="19064at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="SSF48371"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF96108.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="570" mass="68189" checksum="A55B8602D27B7039" modified="2011-07-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>F6BC30</accession>
    <name>F6BC30_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">ABC transporter, ATPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_0557</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96111.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798718.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0557"/>
    <dbReference type="EnsemblBacteria" id="AEF96111">
        <property type="protein sequence ID" value="AEF96111"/>
        <property type="gene ID" value="Metig_0557"/>
    </dbReference>
    <dbReference type="GeneID" id="10643395"/>
    <dbReference type="KEGG" id="mig:Metig_0557"/>
    <dbReference type="HOGENOM" id="CLU_598017_0_0_2"/>
    <dbReference type="OMA" id="MAYQIQR"/>
    <dbReference type="OrthoDB" id="60568at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019195">
        <property type="entry name" value="ABC_ATPase_put"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38149">
        <property type="entry name" value="PTHR38149"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09818">
        <property type="entry name" value="ABC_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF96111.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="449" mass="51292" checksum="430556A6B0CEF2C1" modified="2011-07-27" version="1">MRENKFSLIDEIISRIKTEHLDRKKLTKSNKNKLFYVAKQADGKLKVTLPFVFKREDFLDLNKYGIEGATGHIIERIKDCIRRGDFSILSGNLAKRYEALYEPVTVVNCDLNIGSDLWRADRYNYIKNENLHLLLRMIFDDENAKTIATEIDELVGKLNEFIQKIPYKDLKKETLNIVNQKDLRNRLDKLGLVCFIGDGSKPAREYTRIRRHYRIAGPKKGVNVEFKTPIELNPIEIELFDGNVITGLGIKKREIFIITGRNAQGKSTLLEAIESGQDDHLIGDGREYIITTNKVSKATTGTMQMNGQDISLFFQKLPKGIKGNPKKVYGTASGSMTMAYQIQRALNTKMDLILIDEDNSAVNLLVSGLLSKWFEGVKSLAEIIMNEREKLTSAFIIVTSSLDLLTACGDRAIYLEDHEAKYLDLKKFRRELGKYYLNLGTELYNKKEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>F6BC49</accession>
    <name>F6BC49_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">3-isopropylmalate dehydratase large subunit</fullName>
            <ecNumber evidence="6">4.2.1.33</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Alpha-IPM isomerase</fullName>
            <shortName evidence="6">IPMI</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Isopropylmalate isomerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">leuC</name>
        <name evidence="8" type="ordered locus">Metig_0576</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>(2R,3S)-3-isopropylmalate = (2S)-2-isopropylmalate</text>
            <dbReference type="Rhea" id="RHEA:32287"/>
            <dbReference type="ChEBI" id="CHEBI:1178"/>
            <dbReference type="ChEBI" id="CHEBI:35121"/>
            <dbReference type="EC" id="4.2.1.33"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="6">Binds 1 [4Fe-4S] cluster per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="6">Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 2/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Heterodimer of LeuC and LeuD.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the aconitase/IPM isomerase family. LeuC type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.33" evidence="6"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96130.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798737.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0576"/>
    <dbReference type="EnsemblBacteria" id="AEF96130">
        <property type="protein sequence ID" value="AEF96130"/>
        <property type="gene ID" value="Metig_0576"/>
    </dbReference>
    <dbReference type="GeneID" id="10643414"/>
    <dbReference type="KEGG" id="mig:Metig_0576"/>
    <dbReference type="HOGENOM" id="CLU_006714_3_4_2"/>
    <dbReference type="OMA" id="CNMSIEM"/>
    <dbReference type="OrthoDB" id="15714at2157"/>
    <dbReference type="UniPathway" id="UPA00048">
        <property type="reaction ID" value="UER00071"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003861">
        <property type="term" value="F:3-isopropylmalate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009098">
        <property type="term" value="P:leucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01583">
        <property type="entry name" value="IPMI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.499.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01027">
        <property type="entry name" value="LeuC_type2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015931">
        <property type="entry name" value="Acnase/IPM_dHydase_lsu_aba_1/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001030">
        <property type="entry name" value="Acoase/IPM_deHydtase_lsu_aba"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018136">
        <property type="entry name" value="Aconitase_4Fe-4S_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036008">
        <property type="entry name" value="Aconitase_4Fe-4S_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011826">
        <property type="entry name" value="HAcnase/IPMdehydase_lsu_prok"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006251">
        <property type="entry name" value="Homoacnase/IPMdehydase_lsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033941">
        <property type="entry name" value="IPMI_cat"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00330">
        <property type="entry name" value="Aconitase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00415">
        <property type="entry name" value="ACONITASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53732">
        <property type="entry name" value="SSF53732"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01343">
        <property type="entry name" value="hacA_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02086">
        <property type="entry name" value="IPMI_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00450">
        <property type="entry name" value="ACONITASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01244">
        <property type="entry name" value="ACONITASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 6" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="6" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="3 6" id="KW-0408">Iron</keyword>
    <keyword evidence="4 6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="6" id="KW-0432">Leucine biosynthesis</keyword>
    <keyword evidence="5 6 8" id="KW-0456">Lyase</keyword>
    <keyword evidence="2 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aconitase" evidence="7">
        <location>
            <begin position="8"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="304"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="365"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="368"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEF96130.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="424" mass="45934" checksum="4C0A0D0938E564E5" modified="2011-07-27" version="1">MGMTLAEKILAKASGKKEVSPGDIVMAKIETAMVHDITGPLTVNTLKKEGIKKVWDPEKIVVLFDHQVPADSINAAENHILMRKFVKEQGIKYFYDIREGVCHQVLPEKGHVVPGTVLVGADSHTCTHGAFGAFATGIGSTDMAAVFATGELWFKVPETLYFNITGELKPYVMSKDVILHIIGEVGADGATYKACQFGGETVKNMSIASRMTMTNMAIEMGGKTGLIEPDEKTIQYVKNAMKKHGTEKPFEVIKADEDAEYEEVFEVEVNDLEPMIACPHNVDNVKAVREVAGTPIDQVFIGSCTNGRLEDLRIAIKIIEEHGGIADDVRVIVTPASREVMLKAMEEGIIQKFYKYGCVVTNPSCSACMGALYGILGPGEVGVATSNRNFRGREGSLDSEVYLVSPATAAACAVKGEITDPRDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>F6BC80</accession>
    <name>F6BC80_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="3">S-layer protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_1754</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97286.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799875.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1754"/>
    <dbReference type="EnsemblBacteria" id="AEF97286">
        <property type="protein sequence ID" value="AEF97286"/>
        <property type="gene ID" value="Metig_1754"/>
    </dbReference>
    <dbReference type="GeneID" id="10644628"/>
    <dbReference type="KEGG" id="mig:Metig_1754"/>
    <dbReference type="HOGENOM" id="CLU_016296_0_0_2"/>
    <dbReference type="OMA" id="LMFKEGT"/>
    <dbReference type="OrthoDB" id="37561at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022651">
        <property type="entry name" value="S_layer_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006454">
        <property type="entry name" value="S_layer_MJ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022650">
        <property type="entry name" value="S_layer_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05124">
        <property type="entry name" value="S_layer_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05123">
        <property type="entry name" value="S_layer_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01564">
        <property type="entry name" value="S_layer_MJ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="S_layer_N" evidence="1">
        <location>
            <begin position="6"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="S_layer_N" evidence="1">
        <location>
            <begin position="134"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="domain" description="S_layer_C" evidence="2">
        <location>
            <begin position="303"/>
            <end position="523"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF97286.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="524" mass="56168" checksum="A39F0EC08BBFDFA0" modified="2011-07-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCA8</accession>
    <name>F6BCA8_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Protein FdhD</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">fdhD</name>
        <name evidence="4" type="ordered locus">Metig_1782</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Required for formate dehydrogenase (FDH) activity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the FdhD family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97314.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799903.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1782"/>
    <dbReference type="EnsemblBacteria" id="AEF97314">
        <property type="protein sequence ID" value="AEF97314"/>
        <property type="gene ID" value="Metig_1782"/>
    </dbReference>
    <dbReference type="GeneID" id="10644659"/>
    <dbReference type="KEGG" id="mig:Metig_1782"/>
    <dbReference type="HOGENOM" id="CLU_056887_4_2_2"/>
    <dbReference type="OMA" id="WAALFDF"/>
    <dbReference type="OrthoDB" id="68783at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016783">
        <property type="term" value="F:sulfurtransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006777">
        <property type="term" value="P:Mo-molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00187">
        <property type="entry name" value="FdhD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016193">
        <property type="entry name" value="Cytidine_deaminase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003786">
        <property type="entry name" value="FdhD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30592">
        <property type="entry name" value="PTHR30592"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02634">
        <property type="entry name" value="FdhD-NarQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015626">
        <property type="entry name" value="FdhD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53927">
        <property type="entry name" value="SSF53927"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00129">
        <property type="entry name" value="fdhD_narQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 3" id="KW-0501">Molybdenum cofactor biosynthesis</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Mo-bisPGD binding" evidence="3">
        <location>
            <begin position="211"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEF97314.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="229" mass="25673" checksum="6D24CB009C0D6101" modified="2011-07-27" version="1">MITKVNAYRWEGQATPIEDYVCVEEIYELYINNKFIEELVASPNQIKELGIGHAICEGYVSKNSIINVHLDENKIYVESKEIKGDKDTEKDTQIKINVDTILKIIKTMPTLSKIWELTGGVHWAALFNTSGEVIAFSEDIGRHNAVDKVVGYAILNNINLGNCILASSGRQPYAMVKKLVNANIPIVITKSPPTDKGVKLARENNITLIGFARKNRFTIYSGKDRIVFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCB6</accession>
    <name>F6BCB6_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 16">Riboflavin kinase</fullName>
            <shortName evidence="16">RFK</shortName>
            <ecNumber evidence="4 16">2.7.1.161</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14 16">CTP-dependent riboflavin kinase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15 16">CTP:riboflavin 5'-phosphotransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13 16">Flavokinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">ribK</name>
        <name evidence="18" type="ordered locus">Metig_1790</name>
    </gene>
    <organism evidence="19">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="16">Catalyzes the CTP-dependent phosphorylation of riboflavin (vitamin B2) to form flavin mononucleotide (FMN).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 16">
            <text>CTP + riboflavin = CDP + FMN + H(+)</text>
            <dbReference type="Rhea" id="RHEA:25021"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:57986"/>
            <dbReference type="ChEBI" id="CHEBI:58069"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="EC" id="2.7.1.161"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="16">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="16">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 16">Cofactor biosynthesis; FMN biosynthesis; FMN from riboflavin (CTP route): step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 16">Belongs to the archaeal riboflavin kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.161" evidence="4 16"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97322.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799911.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1790"/>
    <dbReference type="EnsemblBacteria" id="AEF97322">
        <property type="protein sequence ID" value="AEF97322"/>
        <property type="gene ID" value="Metig_1790"/>
    </dbReference>
    <dbReference type="GeneID" id="10644667"/>
    <dbReference type="KEGG" id="mig:Metig_1790"/>
    <dbReference type="HOGENOM" id="CLU_140165_0_0_2"/>
    <dbReference type="OMA" id="PYEGTLN"/>
    <dbReference type="OrthoDB" id="91297at2157"/>
    <dbReference type="UniPathway" id="UPA00276">
        <property type="reaction ID" value="UER00929"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008531">
        <property type="term" value="F:riboflavin kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009398">
        <property type="term" value="P:FMN biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009231">
        <property type="term" value="P:riboflavin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01285">
        <property type="entry name" value="Riboflavin_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039063">
        <property type="entry name" value="RibK_CTP-dep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023470">
        <property type="entry name" value="Riboflavin_kinase_archaeal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023602">
        <property type="entry name" value="Riboflavin_kinase_CTP-dep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023465">
        <property type="entry name" value="Riboflavin_kinase_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40706">
        <property type="entry name" value="PTHR40706"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01982">
        <property type="entry name" value="CTP-dep_RFKase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82114">
        <property type="entry name" value="SSF82114"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 16" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="7 16" id="KW-0288">FMN</keyword>
    <keyword evidence="11 16 18" id="KW-0418">Kinase</keyword>
    <keyword evidence="12 16" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9 16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 16 18" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="CTP-dep_RFKase" evidence="17">
        <location>
            <begin position="7"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <begin position="10"/>
            <end position="15"/>
        </location>
        <ligand>
            <name>CDP</name>
            <dbReference type="ChEBI" id="CHEBI:58069"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="39"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="41"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="97"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="98"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="105"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <begin position="110"/>
            <end position="113"/>
        </location>
        <ligand>
            <name>CDP</name>
            <dbReference type="ChEBI" id="CHEBI:58069"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006428"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00017394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00029789"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00030544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00033116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF01982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="AEF97322.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="134" mass="15424" checksum="A088B363388E8AFE" modified="2011-07-27" version="1">MEVIGKVVSGRGEGRYYVSLPPYKRRFKKILGFIPYPGTLNVKLDEALDIDKLNPIETDDFIYNDKKYFGVKIIPVKISTFKHDLEIEGAIIVPKKTYHPNNIVEIISPVKLRDILSLNDGDWVKIKIKYSCVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCD4</accession>
    <name>F6BCD4_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Archaeoflavoprotein, MJ0208 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0591</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96145.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0591"/>
    <dbReference type="EnsemblBacteria" id="AEF96145">
        <property type="protein sequence ID" value="AEF96145"/>
        <property type="gene ID" value="Metig_0591"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0591"/>
    <dbReference type="HOGENOM" id="CLU_098523_0_0_2"/>
    <dbReference type="OMA" id="IVWCITG"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016645">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-NH group of donors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901285">
        <property type="term" value="P:5,6,7,8-tetrahydromethanopterin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1950">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014073">
        <property type="entry name" value="DmrX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036551">
        <property type="entry name" value="Flavin_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003382">
        <property type="entry name" value="Flavoprotein"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00037">
        <property type="entry name" value="Fer4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02441">
        <property type="entry name" value="Flavoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52507">
        <property type="entry name" value="SSF52507"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02700">
        <property type="entry name" value="flavo_MJ0208"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="176"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="211"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96145.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="279" mass="31448" checksum="43FEEA37D449DADE" modified="2011-07-27" version="1">MFIYVEKILPKNIYILVGVGHLFIFISKYQTHFGGVMKVVWCITGAGHLLRESFEVMKKLKKHGIKITTLVSRAGEEVVKMYGLMEDLYKISNNNYYEELIFEREHPYSAPITGRLSLGKYDYLISTPTTGNTVAKVVNGIADTLVTNAIAQAGKGFVKCIIVPVDYEEGIVTTRLPFSIDREKCTLCKACVEVCKNNAIVVNFDETSSKSFMEIILSKCVGCGKCKEICQYDAVIAGKEIKMRVRKIDAENTRKLKDLEDTIVLKHPYEIIDILLKKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCH4</accession>
    <name>F6BCH4_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">D-3-phosphoglycerate dehydrogenase</fullName>
            <ecNumber evidence="5">1.1.1.95</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Metig_0635</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 5">
            <text>(2R)-3-phosphoglycerate + NAD(+) = 3-phosphooxypyruvate + H(+) + NADH</text>
            <dbReference type="Rhea" id="RHEA:12641"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:18110"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:58272"/>
            <dbReference type="EC" id="1.1.1.95"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 5">Amino-acid biosynthesis; L-serine biosynthesis; L-serine from 3-phospho-D-glycerate: step 1/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 5">Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.95" evidence="5"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96185.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798790.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0635"/>
    <dbReference type="EnsemblBacteria" id="AEF96185">
        <property type="protein sequence ID" value="AEF96185"/>
        <property type="gene ID" value="Metig_0635"/>
    </dbReference>
    <dbReference type="GeneID" id="10643474"/>
    <dbReference type="KEGG" id="mig:Metig_0635"/>
    <dbReference type="HOGENOM" id="CLU_019796_8_1_2"/>
    <dbReference type="OMA" id="NIAGMQV"/>
    <dbReference type="OrthoDB" id="24697at2157"/>
    <dbReference type="UniPathway" id="UPA00135">
        <property type="reaction ID" value="UER00196"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004617">
        <property type="term" value="F:phosphoglycerate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006564">
        <property type="term" value="P:L-serine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002912">
        <property type="entry name" value="ACT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029009">
        <property type="entry name" value="ASB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006139">
        <property type="entry name" value="D-isomer_2_OHA_DH_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029753">
        <property type="entry name" value="D-isomer_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029752">
        <property type="entry name" value="D-isomer_DH_CS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006140">
        <property type="entry name" value="D-isomer_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006236">
        <property type="entry name" value="PGDH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045626">
        <property type="entry name" value="PGDH_ASB_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00389">
        <property type="entry name" value="2-Hacid_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02826">
        <property type="entry name" value="2-Hacid_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01842">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19304">
        <property type="entry name" value="PGDH_inter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143548">
        <property type="entry name" value="SSF143548"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="SSF55021"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01327">
        <property type="entry name" value="PGDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51671">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00065">
        <property type="entry name" value="D_2_HYDROXYACID_DH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00670">
        <property type="entry name" value="D_2_HYDROXYACID_DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00671">
        <property type="entry name" value="D_2_HYDROXYACID_DH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="5" id="KW-0520">NAD</keyword>
    <keyword evidence="4 5 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0718">Serine biosynthesis</keyword>
    <feature type="domain" description="ACT" evidence="6">
        <location>
            <begin position="451"/>
            <end position="523"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005854"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEF96185.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="523" mass="56696" checksum="B1223189C2526192" modified="2011-07-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCL1</accession>
    <name>F6BCL1_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_0672</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96222.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798825.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0672"/>
    <dbReference type="EnsemblBacteria" id="AEF96222">
        <property type="protein sequence ID" value="AEF96222"/>
        <property type="gene ID" value="Metig_0672"/>
    </dbReference>
    <dbReference type="GeneID" id="10643512"/>
    <dbReference type="KEGG" id="mig:Metig_0672"/>
    <dbReference type="HOGENOM" id="CLU_110112_1_0_2"/>
    <dbReference type="OMA" id="KHERSFK"/>
    <dbReference type="OrthoDB" id="117224at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012041">
        <property type="entry name" value="Znf_CPxCG-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42195">
        <property type="entry name" value="PTHR42195"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19769">
        <property type="entry name" value="CPxCG_zf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015877">
        <property type="entry name" value="UCP015877"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF96222.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="205" mass="23797" checksum="56764659F8F7969A" modified="2011-07-27" version="1">MEHFECPVCEELTPHEILKSKESKKHAKYIVKCLECNTIHEVEFSVKLKDVSVIISRYDESERKIIQLPMGEVVKVGEELEVDGERIEVTTIDTDDGKRVSSSKVDDIKTIWAKSLDIPKKLGISINDGRRTYSVYIFVPMDYVFEVGNVYRINNGFFRVKLIKAEKGHVKRDVAKNIKRVYADLIKPTRRYIDLTEYLPDIEEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCL4</accession>
    <name>F6BCL4_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uroporphyrinogen decarboxylase (URO-D)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0675</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96225.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798828.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0675"/>
    <dbReference type="EnsemblBacteria" id="AEF96225">
        <property type="protein sequence ID" value="AEF96225"/>
        <property type="gene ID" value="Metig_0675"/>
    </dbReference>
    <dbReference type="GeneID" id="10643515"/>
    <dbReference type="KEGG" id="mig:Metig_0675"/>
    <dbReference type="HOGENOM" id="CLU_040933_2_0_2"/>
    <dbReference type="OMA" id="CGDINPI"/>
    <dbReference type="OrthoDB" id="21172at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004853">
        <property type="term" value="F:uroporphyrinogen decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006779">
        <property type="term" value="P:porphyrin-containing compound biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038071">
        <property type="entry name" value="UROD/MetE-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000257">
        <property type="entry name" value="Uroporphyrinogen_deCOase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01208">
        <property type="entry name" value="URO-D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51726">
        <property type="entry name" value="SSF51726"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Uroporphyrinogen_deCOase" evidence="1">
        <location>
            <begin position="34"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96225.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="314" mass="35676" checksum="7658BECF08705885" modified="2011-07-27" version="1">MIDIEEEVKNRTLIAPMGGCYIAKLYGCGKKHYFCRNGEIFGKALCKAKEYFGYDIIHLSSDIYVEVEAMGLYSWSCIYKEYGKMNENFDIDSLTINLNSGRVSEYRKAIEYCNSKNNTVFVSVSAPFTLAAIIRGIDRFMIDLIKNKKFAKDLIKKTTEISKIVIEDIKDIAYPFVVDAVASSSLISPEFYREFAFKPVKKVLKLCDFPILHMCGDINPILDDIKKTGSKVISFDDNLEISKAFEVFNDLIVFGNINPSMMYHSNVDWVKTEVKKCMESKTGDYILSTGCSIPVGTPKENLKTLVRYGRCYDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCM1</accession>
    <name>F6BCM1_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transketolase</fullName>
            <ecNumber evidence="2">2.2.1.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0682</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.2.1.1" evidence="2"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96232.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798835.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0682"/>
    <dbReference type="EnsemblBacteria" id="AEF96232">
        <property type="protein sequence ID" value="AEF96232"/>
        <property type="gene ID" value="Metig_0682"/>
    </dbReference>
    <dbReference type="GeneID" id="10643522"/>
    <dbReference type="KEGG" id="mig:Metig_0682"/>
    <dbReference type="HOGENOM" id="CLU_009227_4_1_2"/>
    <dbReference type="OMA" id="MVHAVNS"/>
    <dbReference type="OrthoDB" id="73908at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004802">
        <property type="term" value="F:transketolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006082">
        <property type="term" value="P:organic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901576">
        <property type="term" value="P:organic substance biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044272">
        <property type="term" value="P:sulfur compound biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="CDD" id="cd02012">
        <property type="entry name" value="TPP_TK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005474">
        <property type="entry name" value="Transketolase_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00456">
        <property type="entry name" value="Transketolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="SSF52518"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00801">
        <property type="entry name" value="TRANSKETOLASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="TRANSKETOLASE_1" evidence="1">
        <location>
            <begin position="14"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS00801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96232.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="269" mass="30039" checksum="3BC19FC59F942FFE" modified="2011-07-27" version="1">MEIEELKKIAKRVRYNIVKMVGLAKSGHPGGSLSSTEIIVALYFKIMKHDPKNPKMKDRDRFVLSKGHSCPALYAVLAECGYFDKEELWKLRKLDGLLQGHPSIDIPGIEMSTGSLGQGFSAAVGMALGCKLDKLNNYVYVLLGDGECQEGQVWEAAMAAAHYKLDNLIGFVDRNMLQIDGCTEDVMSLGDLKEKFKAFGWDVFEIDGHDFEQIINTVEKAKKMKNRKPKMIIAYTTKGKGVSFMEDNVGFHGKAPNEEQLEIALKELE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCM8</accession>
    <name>F6BCM8_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Cytochrome c biogenesis protein transmembrane region</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_0689</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96239.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798842.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0689"/>
    <dbReference type="EnsemblBacteria" id="AEF96239">
        <property type="protein sequence ID" value="AEF96239"/>
        <property type="gene ID" value="Metig_0689"/>
    </dbReference>
    <dbReference type="GeneID" id="10643529"/>
    <dbReference type="KEGG" id="mig:Metig_0689"/>
    <dbReference type="HOGENOM" id="CLU_053225_5_0_2"/>
    <dbReference type="OMA" id="TSLTPCM"/>
    <dbReference type="OrthoDB" id="96261at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017004">
        <property type="term" value="P:cytochrome complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003834">
        <property type="entry name" value="Cyt_c_assmbl_TM_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02683">
        <property type="entry name" value="DsbD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="41"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="76"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="124"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="159"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="199"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="domain" description="DsbD" evidence="2">
        <location>
            <begin position="6"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF96239.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="218" mass="23871" checksum="FD5797BF7A1F3105" modified="2011-07-27" version="1">MDLLLIFFAGLFTALGPCVITILPISLAYTFGVSNSKKEGFIVSLFFVLGLSLIFSLLGVISAVFGSTFDLYKLKFIAGILAIAFGFSTLFNYKLSILTDSLNKFRSYLNKAKNKKFQFSSLKYLNAFLFGFFYGVTANTCADPVLITILSYTATKSDILFGFIALFIYSIGFGIPIIILSTLGAEGKSIINKITKPRVINIISGVILIVLGIYVIFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCM9</accession>
    <name>F6BCM9_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Thioredoxin domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0690</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96240.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798843.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0690"/>
    <dbReference type="EnsemblBacteria" id="AEF96240">
        <property type="protein sequence ID" value="AEF96240"/>
        <property type="gene ID" value="Metig_0690"/>
    </dbReference>
    <dbReference type="GeneID" id="10643530"/>
    <dbReference type="KEGG" id="mig:Metig_0690"/>
    <dbReference type="HOGENOM" id="CLU_090389_10_4_2"/>
    <dbReference type="OMA" id="MKHFLNF"/>
    <dbReference type="OrthoDB" id="110723at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="1">
        <location>
            <begin position="11"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96240.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="126" mass="14200" checksum="78E4463C10A4E616" modified="2011-07-27" version="1">MNLKIFLFPILILGLSVPFSGCVEDSSVNNGGIGINEGRSDFNGTCVVEFYADWCIYCKKLEPVLDELEKEGIKVVRINVDENKELASKYGVRYLPTVFYVEDGKIVDKTIGYNPEEIKEKAKQLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCP5</accession>
    <name>F6BCP5_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Thiamine_BP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_0706</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0045 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96256.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798859.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0706"/>
    <dbReference type="EnsemblBacteria" id="AEF96256">
        <property type="protein sequence ID" value="AEF96256"/>
        <property type="gene ID" value="Metig_0706"/>
    </dbReference>
    <dbReference type="GeneID" id="10643546"/>
    <dbReference type="KEGG" id="mig:Metig_0706"/>
    <dbReference type="HOGENOM" id="CLU_137479_3_2_2"/>
    <dbReference type="OMA" id="TPMGTVF"/>
    <dbReference type="OrthoDB" id="126214at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.930">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029756">
        <property type="entry name" value="MTH1187/YkoF-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002767">
        <property type="entry name" value="Thiamine_BP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01910">
        <property type="entry name" value="Thiamine_BP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89957">
        <property type="entry name" value="SSF89957"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00106">
        <property type="entry name" value="TIGR00106"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thiamine_BP" evidence="2">
        <location>
            <begin position="4"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF96256.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="95" mass="10734" checksum="6387EE5B9D16B04D" modified="2011-07-27" version="1">MVVAEVSIVPIGEGPSVSKYVKKAIEVFKKYNLKVEPHAMGTVLEGDLEEILEAFKEAHREVLKDSKRVLSTLKIDERTDKENTIERKLRAIGIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCR1</accession>
    <name>F6BCR1_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Regulatory protein Crp</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0722</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96272.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798875.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0722"/>
    <dbReference type="EnsemblBacteria" id="AEF96272">
        <property type="protein sequence ID" value="AEF96272"/>
        <property type="gene ID" value="Metig_0722"/>
    </dbReference>
    <dbReference type="GeneID" id="10643562"/>
    <dbReference type="KEGG" id="mig:Metig_0722"/>
    <dbReference type="HOGENOM" id="CLU_090237_0_0_2"/>
    <dbReference type="OMA" id="PHIKQKE"/>
    <dbReference type="OrthoDB" id="41689at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012318">
        <property type="entry name" value="HTH_CRP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012015">
        <property type="entry name" value="UCP_HTH_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004955">
        <property type="entry name" value="HTH_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00419">
        <property type="entry name" value="HTH_CRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH crp-type" evidence="1">
        <location>
            <begin position="14"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96272.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="260" mass="29221" checksum="80710C9559B7D270" modified="2011-07-27" version="1">MKKRNITEFQILSEIVRKQPHVKQKEIADALDITIQAVSEHMRNLLKKGYIKSRGRGEYIITDKGMRVLKNWISEFKRYLDDVTQDLYKYKDVWPAIANEDLNEGDEVYLFMRKGLLYASKEIKTDAKGKVLEGGKKGEDVAICDIKGIIDVPRGKVTIIKIPPRIKGGSKNTNYELIKKVLEENEDVAIATMGTVANVVASKMGLHPDIRFAVSEGIVAACNRGCDVLALITGKMTEKVVRVLDKHKIGYTVLDATKED</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCV7</accession>
    <name>F6BCV7_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">50S ribosomal protein L10e</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">rpl10e</name>
        <name evidence="6" type="ordered locus">Metig_0772</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the universal ribosomal protein uL16 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96318.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0772"/>
    <dbReference type="EnsemblBacteria" id="AEF96318">
        <property type="protein sequence ID" value="AEF96318"/>
        <property type="gene ID" value="Metig_0772"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0772"/>
    <dbReference type="HOGENOM" id="CLU_084051_0_2_2"/>
    <dbReference type="OMA" id="HHVIREN"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01433">
        <property type="entry name" value="Ribosomal_L16_L10e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1170.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00448">
        <property type="entry name" value="Ribosomal_L10e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001197">
        <property type="entry name" value="Ribosomal_L10e"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016180">
        <property type="entry name" value="Ribosomal_L10e/L16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036920">
        <property type="entry name" value="Ribosomal_L10e/L16_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022981">
        <property type="entry name" value="Ribosomal_L10e_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018255">
        <property type="entry name" value="Ribosomal_L10e_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11726">
        <property type="entry name" value="PTHR11726"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00252">
        <property type="entry name" value="Ribosomal_L16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005590">
        <property type="entry name" value="Ribosomal_L10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54686">
        <property type="entry name" value="SSF54686"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00279">
        <property type="entry name" value="uL16_euk_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01257">
        <property type="entry name" value="RIBOSOMAL_L10E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 6" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Ribosomal_L16" evidence="5">
        <location>
            <begin position="14"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00252"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEF96318.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="173" mass="19724" checksum="79E7D3137675DE3D" modified="2011-07-27" version="1">MALRPGKCYRGVDKPPYTRKEYIRGVPQPKIVHYIMGDPRRDFPIQMSLVVKEPVQIRHNALEAARVAGNKYLMKKCGRMGYRFQIRVYPHQVLREHKMACGAGADRISDGMRLAFGKPIGTAARVRKNQPVITVWVDPDKVNDAKEALRRCAMKLPVSCKIVIEKGKELIKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCV8</accession>
    <name>F6BCV8_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Phosphoenolpyruvate synthase</fullName>
            <shortName evidence="7">PEP synthase</shortName>
            <ecNumber evidence="7">2.7.9.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Pyruvate, water dikinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">Metig_0773</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3 7">Catalyzes the phosphorylation of pyruvate to phosphoenolpyruvate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>ATP + H2O + pyruvate = AMP + 2 H(+) + phosphate + phosphoenolpyruvate</text>
            <dbReference type="Rhea" id="RHEA:11364"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.7.9.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 7">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4 7">Carbohydrate biosynthesis; gluconeogenesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5 7">Belongs to the PEP-utilizing enzyme family.</text>
    </comment>
    <dbReference type="EC" id="2.7.9.2" evidence="7"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96319.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798922.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0773"/>
    <dbReference type="EnsemblBacteria" id="AEF96319">
        <property type="protein sequence ID" value="AEF96319"/>
        <property type="gene ID" value="Metig_0773"/>
    </dbReference>
    <dbReference type="GeneID" id="10643613"/>
    <dbReference type="KEGG" id="mig:Metig_0773"/>
    <dbReference type="HOGENOM" id="CLU_007308_6_2_2"/>
    <dbReference type="OMA" id="RRFVQMY"/>
    <dbReference type="OrthoDB" id="3139at2157"/>
    <dbReference type="UniPathway" id="UPA00138"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008986">
        <property type="term" value="F:pyruvate, water dikinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006090">
        <property type="term" value="P:pyruvate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008279">
        <property type="entry name" value="PEP-util_enz_mobile_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006319">
        <property type="entry name" value="PEP_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018274">
        <property type="entry name" value="PEP_util_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000121">
        <property type="entry name" value="PEP_util_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023151">
        <property type="entry name" value="PEP_util_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036637">
        <property type="entry name" value="Phosphohistidine_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002192">
        <property type="entry name" value="PPDK_AMP/ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015813">
        <property type="entry name" value="Pyrv/PenolPyrv_Kinase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040442">
        <property type="entry name" value="Pyrv_Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43030">
        <property type="entry name" value="PTHR43030"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00391">
        <property type="entry name" value="PEP-utilizers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02896">
        <property type="entry name" value="PEP-utilizers_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01326">
        <property type="entry name" value="PPDK_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000854">
        <property type="entry name" value="PEP_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51621">
        <property type="entry name" value="SSF51621"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52009">
        <property type="entry name" value="SSF52009"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01418">
        <property type="entry name" value="PEP_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00742">
        <property type="entry name" value="PEP_ENZYMES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00370">
        <property type="entry name" value="PEP_ENZYMES_PHOS_SITE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PPDK_N" evidence="9">
        <location>
            <begin position="17"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="domain" description="PEP-utilizers" evidence="8">
        <location>
            <begin position="365"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="domain" description="PEP-utilizers_C" evidence="10">
        <location>
            <begin position="460"/>
            <end position="750"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001518"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00002988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00007837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR000854"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AEF96319.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="763" mass="84267" checksum="FFFC0376FA9AFA18" modified="2011-07-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2020-06-17" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCW7</accession>
    <name>F6BCW7_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_0782</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96328.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96328">
        <property type="protein sequence ID" value="AEF96328"/>
        <property type="gene ID" value="Metig_0782"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0782"/>
    <dbReference type="HOGENOM" id="CLU_3194505_0_0_2"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF96328.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="45" mass="4958" checksum="C1B6659E5731855C" modified="2011-07-27" version="1">MMPLMKVDTGDLRRRTLLSVKPMGGAGVLPERSDGNLKTVRFLCP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCX1</accession>
    <name>F6BCX1_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Probable inosine/xanthosine triphosphatase</fullName>
            <shortName evidence="2">ITPase/XTPase</shortName>
            <ecNumber evidence="2">3.6.1.73</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">Non-canonical purine NTP phosphatase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="2">Non-standard purine NTP phosphatase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="2">Nucleoside-triphosphate phosphatase</fullName>
            <shortName evidence="2">NTPase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metig_0787</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Phosphatase that hydrolyzes non-canonical purine nucleotides such as XTP and ITP to their respective diphosphate derivatives. Probably excludes non-canonical purines from DNA/RNA precursor pool, thus preventing their incorporation into DNA/RNA and avoiding chromosomal lesions.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>H2O + ITP = H(+) + IDP + phosphate</text>
            <dbReference type="Rhea" id="RHEA:28330"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58280"/>
            <dbReference type="ChEBI" id="CHEBI:61402"/>
            <dbReference type="EC" id="3.6.1.73"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>H2O + XTP = H(+) + phosphate + XDP</text>
            <dbReference type="Rhea" id="RHEA:28406"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:59884"/>
            <dbReference type="ChEBI" id="CHEBI:61314"/>
            <dbReference type="EC" id="3.6.1.73"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="2">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="2">Binds 1 divalent metal cation per subunit; can use either Mg(2+) or Mn(2+).</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the YjjX NTPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.73" evidence="2"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96332.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0787"/>
    <dbReference type="EnsemblBacteria" id="AEF96332">
        <property type="protein sequence ID" value="AEF96332"/>
        <property type="gene ID" value="Metig_0787"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0787"/>
    <dbReference type="HOGENOM" id="CLU_087417_0_1_2"/>
    <dbReference type="OMA" id="ADYWVGI"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0103023">
        <property type="term" value="F:ITPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017111">
        <property type="term" value="F:nucleoside-triphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009117">
        <property type="term" value="P:nucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.950.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00648">
        <property type="entry name" value="Non_canon_purine_NTPase_YjjX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029001">
        <property type="entry name" value="ITPase-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002786">
        <property type="entry name" value="Non_canon_purine_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026533">
        <property type="entry name" value="NTPase/PRRC1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01931">
        <property type="entry name" value="NTPase_I-T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52972">
        <property type="entry name" value="SSF52972"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00258">
        <property type="entry name" value="TIGR00258"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0460">Magnesium</keyword>
    <keyword evidence="1 2" id="KW-0464">Manganese</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0546">Nucleotide metabolism</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NTPase_I-T" evidence="3">
        <location>
            <begin position="70"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="2">
        <location>
            <begin position="71"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="99"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEF96332.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="242" mass="26569" checksum="9A95237BC0612638" modified="2011-07-27" version="1">MSIFDHVGRTPYTNNGENMDFKDKKCEICGKKAETFMFGRFLCLDEKCIEEARRLRGGPAGHKLRVIAVGSTNPVKIRAVEEGMRKILGSVEVVGVDVDSGVSKHPIGFEETYRGALNRAKNALNKQSALYGVGIEAGLIEVDGHYLDIHVCVVFDGLTATVGTSQGFEYPKEVAEAIKKGIEGGIIAEKISGIKDIGKKNGLIGYLTDDNITRLDLCRDAVIMAMIPRMRKNVNLYDLFLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCX2</accession>
    <name>F6BCX2_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Transposase, IS605 OrfB family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Metig_0788</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">In the C-terminal section; belongs to the transposase 35 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96333.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798936.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0788"/>
    <dbReference type="EnsemblBacteria" id="AEF96333">
        <property type="protein sequence ID" value="AEF96333"/>
        <property type="gene ID" value="Metig_0788"/>
    </dbReference>
    <dbReference type="GeneID" id="10643628"/>
    <dbReference type="KEGG" id="mig:Metig_0788"/>
    <dbReference type="HOGENOM" id="CLU_032903_3_4_2"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032196">
        <property type="term" value="P:transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001959">
        <property type="entry name" value="Transposase_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010095">
        <property type="entry name" value="Transposase_IS605_OrfB_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01385">
        <property type="entry name" value="OrfB_IS605"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07282">
        <property type="entry name" value="OrfB_Zn_ribbon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01766">
        <property type="entry name" value="tspaseT_teng_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0815">Transposition</keyword>
    <feature type="domain" description="OrfB_IS605" evidence="5">
        <location>
            <begin position="193"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="domain" description="OrfB_Zn_ribbon" evidence="6">
        <location>
            <begin position="304"/>
            <end position="365"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF07282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEF96333.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="396" mass="46990" checksum="ACFF53041A354623" modified="2011-07-27" version="1">MSNKTKSKENQELSYQVVLSYKVSHNYPLKTFLIECKDKLNECIDMIWNNIEYTKKDKPKLPKSNEFKRNLRNKLLENWSYASHYIDGIIKTAYSILQSWASNYKRGYRTKTKPVVERLFVRVKTTLIKYDKERAEIRITIKPRKEYLTLNIKNEWFFDKVKDLTIGEIILKENEAFLTFKDNLNYSDKDIIVGVDSNLKSLDLFHPIEGWIRIDLTELHRLKEVYDRKIDFLKKLLKKCPLRVLRKINRLFERRRNRVKDFLHKLTTQLSRLFPNAIFVFEDLNKRSMYKNKYFNRRIDRANWNGIIEKISYKAIVILVNPAYTSTTCPICGSRMESQEGQVVCYNCSNSFNRQVVGCFNIFKRGLGVIKRFMGGSGVISERSDENLKTVRFLCP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCX5</accession>
    <name>F6BCX5_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ABC transporter related protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Metig_0791</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96336.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0791"/>
    <dbReference type="EnsemblBacteria" id="AEF96336">
        <property type="protein sequence ID" value="AEF96336"/>
        <property type="gene ID" value="Metig_0791"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0791"/>
    <dbReference type="HOGENOM" id="CLU_000604_86_2_2"/>
    <dbReference type="OMA" id="DPMTRLD"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="40"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="291"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="72"/>
            <end position="79"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="328"/>
            <end position="335"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEF96336.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="589" mass="67137" checksum="28B47BAE402224D7" modified="2011-07-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCX6</accession>
    <name>F6BCX6_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Probable molybdenum cofactor guanylyltransferase</fullName>
            <shortName evidence="4">MoCo guanylyltransferase</shortName>
            <ecNumber evidence="4">2.7.7.77</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">GTP:molybdopterin guanylyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">Mo-MPT guanylyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">Molybdopterin guanylyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">Molybdopterin-guanine dinucleotide synthase</fullName>
            <shortName evidence="4">MGD synthase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">mobA</name>
        <name evidence="6" type="ordered locus">Metig_0792</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Transfers a GMP moiety from GTP to Mo-molybdopterin (Mo-MPT) cofactor (Moco or molybdenum cofactor) to form Mo-molybdopterin guanine dinucleotide (Mo-MGD) cofactor.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>GTP + H(+) + Mo-molybdopterin = diphosphate + Mo-molybdopterin guanine dinucleotide</text>
            <dbReference type="Rhea" id="RHEA:34243"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:71302"/>
            <dbReference type="ChEBI" id="CHEBI:71310"/>
            <dbReference type="EC" id="2.7.7.77"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="4">The N-terminal domain determines nucleotide recognition and specific binding, while the C-terminal domain determines the specific binding to the target protein.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the MobA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.77" evidence="4"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96337.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0792"/>
    <dbReference type="EnsemblBacteria" id="AEF96337">
        <property type="protein sequence ID" value="AEF96337"/>
        <property type="gene ID" value="Metig_0792"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0792"/>
    <dbReference type="HOGENOM" id="CLU_055597_2_0_2"/>
    <dbReference type="OMA" id="HENGYIE"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061603">
        <property type="term" value="F:molybdenum cofactor guanylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006777">
        <property type="term" value="P:Mo-molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02503">
        <property type="entry name" value="MobA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00316">
        <property type="entry name" value="MobA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025877">
        <property type="entry name" value="MobA-like_NTP_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013482">
        <property type="entry name" value="Molybde_CF_guanTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12804">
        <property type="entry name" value="NTP_transf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 4" id="KW-0501">Molybdenum cofactor biosynthesis</keyword>
    <keyword evidence="1 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="NTP_transf_3" evidence="5">
        <location>
            <begin position="5"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="8"/>
            <end position="10"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="20"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="77"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF12804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEF96337.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="213" mass="24708" checksum="ABB0F9DBE6C7DDD1" modified="2011-07-27" version="1">MMISAIVLSGGKARRMNGEKAFRVVNDKYLIEYTADLLNEMNIPFVIVFRNDGEKSDDTKNLEKDFFKKYRQTITWDLEGNKGPLMGILSGMRVLNAKWILVLPCDMPLISRDAINNLIKYIDIAEKEKKNCIIPMHENGYIEPLFSLYMRSSMEVLDRMLNKNSSNKNLSMRNFIEHLSPLYVDAKKIDETGRAFININTLEELKRINSKFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCZ0</accession>
    <name>F6BCZ0_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Diadenylate cyclase</fullName>
            <shortName evidence="6">DAC</shortName>
            <ecNumber evidence="6">2.7.7.85</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Cyclic-di-AMP synthase</fullName>
            <shortName evidence="6">c-di-AMP synthase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">dacZ</name>
        <name evidence="8" type="ordered locus">Metig_0806</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Diadenylate cyclase that catalyzes the condensation of 2 ATP molecules into cyclic di-AMP (c-di-AMP). c-di-AMP is a second messenger for intracellular signal transduction involved in the control of important regulatory processes such as osmoregulation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 6">
            <text>2 ATP = 3',3'-c-di-AMP + 2 diphosphate</text>
            <dbReference type="Rhea" id="RHEA:35655"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:71500"/>
            <dbReference type="EC" id="2.7.7.85"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the adenylate cyclase family. DacZ subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.85" evidence="6"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96351.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798953.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0806"/>
    <dbReference type="EnsemblBacteria" id="AEF96351">
        <property type="protein sequence ID" value="AEF96351"/>
        <property type="gene ID" value="Metig_0806"/>
    </dbReference>
    <dbReference type="GeneID" id="10643647"/>
    <dbReference type="KEGG" id="mig:Metig_0806"/>
    <dbReference type="HOGENOM" id="CLU_063222_2_0_2"/>
    <dbReference type="OMA" id="GHESFIF"/>
    <dbReference type="OrthoDB" id="80301at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004016">
        <property type="term" value="F:adenylate cyclase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106408">
        <property type="term" value="F:diadenylate cyclase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019932">
        <property type="term" value="P:second-messenger-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1380.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1700.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00840">
        <property type="entry name" value="DacZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014499">
        <property type="entry name" value="DAC_DacZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036888">
        <property type="entry name" value="DNA_integrity_DisA_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003390">
        <property type="entry name" value="DNA_integrity_scan_DisA_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015795">
        <property type="entry name" value="Pyrv_Knase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036918">
        <property type="entry name" value="Pyrv_Knase_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02457">
        <property type="entry name" value="DAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02887">
        <property type="entry name" value="PK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF019073">
        <property type="entry name" value="UCP019073"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143597">
        <property type="entry name" value="SSF143597"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52935">
        <property type="entry name" value="SSF52935"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51794">
        <property type="entry name" value="DAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0464">Manganese</keyword>
    <keyword evidence="4 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3 6" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DAC" evidence="7">
        <location>
            <begin position="160"/>
            <end position="315"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000877"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEF96351.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="324" mass="36102" checksum="B7B598BB11E84F8E" modified="2011-07-27" version="1">MNRVECIIKHGIELAYDIDADVLMIFTETGKTYKILKKMLKNCENNKNSGKNRSFKFISLKNLPNIDKLKNKKLKIIVTTPNKDTYNWLLKEEGIIPVFLRYRDDDRYSLVKHGVITSLEKNLLNSDDIVVAIVGIPKTPGGIDTITVVEVNRHTTSLKLYELLKSVDDIERKTIEQVLNIALELGREGREGRPVGTIFVIGDTLNVMNQSQQLILNPFAGHNASIFDEKVKGTIKELSPIDGAFIITEDGRVIAAGRYLESKADNIKLPLGLGARHRAAAAISKNTDAIAITVSESGGIVRVFKDGEIVFEVDPRSKSIAYLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>F6BD63</accession>
    <name>F6BD63_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Adenylyl cyclase CyaB</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0881</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96424.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799026.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0881"/>
    <dbReference type="EnsemblBacteria" id="AEF96424">
        <property type="protein sequence ID" value="AEF96424"/>
        <property type="gene ID" value="Metig_0881"/>
    </dbReference>
    <dbReference type="GeneID" id="10643725"/>
    <dbReference type="KEGG" id="mig:Metig_0881"/>
    <dbReference type="HOGENOM" id="CLU_105244_2_0_2"/>
    <dbReference type="OMA" id="QHPCRDF"/>
    <dbReference type="OrthoDB" id="123503at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd07890">
        <property type="entry name" value="CYTH-like_AC_IV-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008173">
        <property type="entry name" value="Adenylyl_cyclase_CyaB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033469">
        <property type="entry name" value="CYTH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023577">
        <property type="entry name" value="CYTH_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21028">
        <property type="entry name" value="PTHR21028"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01928">
        <property type="entry name" value="CYTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01118">
        <property type="entry name" value="CYTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55154">
        <property type="entry name" value="SSF55154"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00318">
        <property type="entry name" value="cyaB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51707">
        <property type="entry name" value="CYTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CYTH" evidence="1">
        <location>
            <begin position="1"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96424.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="180" mass="21241" checksum="904E6204FA638A35" modified="2011-07-27" version="1">MIEVEIKAKINEDELENIKKTLEELGFEKIEVKEQIDIYFNGIDRDFAKTDEALRIRKENDVWFITYKGPKIDKISKTREEIEVKIEDGYKMEKILEKLGFKKVPPIRKIREIYKKEDIEASIDNVDGVGLYLELEKTIKDEKEKDTALNLLMDTLKSLNISSDRLERKSYLELLHKRGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F6BD67</accession>
    <name>F6BD67_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">AAA_15 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0885</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96428.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799030.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96428">
        <property type="protein sequence ID" value="AEF96428"/>
        <property type="gene ID" value="Metig_0885"/>
    </dbReference>
    <dbReference type="GeneID" id="10643729"/>
    <dbReference type="KEGG" id="mig:Metig_0885"/>
    <dbReference type="HOGENOM" id="CLU_040623_1_0_2"/>
    <dbReference type="OMA" id="VWITTHS"/>
    <dbReference type="OrthoDB" id="38516at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041685">
        <property type="entry name" value="AAA_15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13175">
        <property type="entry name" value="AAA_15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA_15" evidence="1">
        <location>
            <begin position="13"/>
            <end position="402"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96428.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="499" mass="57996" checksum="3CEB7378A3EFA7BC" modified="2011-07-27" version="1">MKNENKENYELLTIKVKDFGVIKEGEVKLKPLTIFFGKNNTGKTYMGYLIWGIFENLTYPPFRKFKIINDNNLNFVISKLKSMITNTTQSTEKIQFNEIKLCMEKDYIKRVFNYEGIDFGELEIVLDKKFEYYVITKNFGFMDEKYVDKILMDFLKEGNIKNKKINKLCIGIFENSPWTASFRLIGKNILIGINNKALKEHDRILGHIIIDMCRILLKNMIKNVIYLPASKSGLILSSSLFIDKVFDSPAFFEIYDNSKNMLDTGEKLAFPEPIKNFMKSYLKPEFYNSDVNQDNQDIASLFEDHIIKGELVYDKDMNKLGYVTKNGKSIPIHCASSSVVETIPILKLVKYSNIVKKGTLLIIEEPEAHLHPDAQRIFARAIVKLINKGVYVLLITHSPYILQQINNCMKLYYLEKIDKEKLKAFLNRHDYKEDEILNPRKVAPYLFVDEVDGVKIKELEIIDNEGISYEAFYHILFSLHNETEELRGLIEGDDGNFEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>F6BD80</accession>
    <name>F6BD80_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">30S ribosomal protein S5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">rps5</name>
        <name evidence="8" type="ordered locus">Metig_0898</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">With S4 and S12 plays an important role in translational accuracy.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Part of the 30S ribosomal subunit. Contacts protein S4.</text>
    </comment>
    <comment type="domain">
        <text evidence="4">The N-terminal domain interacts with the head of the 30S subunit; the C-terminal domain interacts with the body and contacts protein S4. The interaction surface between S4 and S5 is involved in control of translational fidelity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4 6">Belongs to the universal ribosomal protein uS5 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96441.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799043.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0898"/>
    <dbReference type="EnsemblBacteria" id="AEF96441">
        <property type="protein sequence ID" value="AEF96441"/>
        <property type="gene ID" value="Metig_0898"/>
    </dbReference>
    <dbReference type="GeneID" id="10643742"/>
    <dbReference type="KEGG" id="mig:Metig_0898"/>
    <dbReference type="HOGENOM" id="CLU_065898_0_1_2"/>
    <dbReference type="OMA" id="KRGCGSW"/>
    <dbReference type="OrthoDB" id="60954at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01307_A">
        <property type="entry name" value="Ribosomal_S5_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000851">
        <property type="entry name" value="Ribosomal_S5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005324">
        <property type="entry name" value="Ribosomal_S5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005711">
        <property type="entry name" value="Ribosomal_S5_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013810">
        <property type="entry name" value="Ribosomal_S5_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018192">
        <property type="entry name" value="Ribosomal_S5_N_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13718">
        <property type="entry name" value="PTHR13718"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00333">
        <property type="entry name" value="Ribosomal_S5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03719">
        <property type="entry name" value="Ribosomal_S5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01020">
        <property type="entry name" value="uS5_euk_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00585">
        <property type="entry name" value="RIBOSOMAL_S5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50881">
        <property type="entry name" value="S5_DSRBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 5" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="4" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="S5 DRBM" evidence="7">
        <location>
            <begin position="51"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEF96441.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="219" mass="24094" checksum="CB186B22F5440AE0" modified="2011-07-27" version="1">MNRRFDIESWEPKTKVGKMVKEGAITDIDYILDNNLPLLEPEIVDVLLPDLEEQVLDVKLVQRMHKSGRRARFRATVVVGNRNGYVGVGMGKAKEVGPAIRKAIAQAKLSIIKIRRGCGSWECGCGLAHSVPFKSRGKCGSVEVELMPAPRGVGLVAGNVAKAVLGLAGVKDVWTKTFGDTRTTYNFAMATFEALKNLNFVRTLDEHKQKLGIIEGRTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>F6BD83</accession>
    <name>F6BD83_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 5">50S ribosomal protein L32e</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">rpl32e</name>
        <name evidence="6" type="ordered locus">Metig_0901</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the eukaryotic ribosomal protein eL32 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96444.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799046.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0901"/>
    <dbReference type="EnsemblBacteria" id="AEF96444">
        <property type="protein sequence ID" value="AEF96444"/>
        <property type="gene ID" value="Metig_0901"/>
    </dbReference>
    <dbReference type="GeneID" id="10643745"/>
    <dbReference type="KEGG" id="mig:Metig_0901"/>
    <dbReference type="HOGENOM" id="CLU_071479_3_1_2"/>
    <dbReference type="OMA" id="HPSGYEE"/>
    <dbReference type="OrthoDB" id="110802at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00513">
        <property type="entry name" value="Ribosomal_L32_L32e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00810">
        <property type="entry name" value="Ribosomal_L32e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001515">
        <property type="entry name" value="Ribosomal_L32e"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023654">
        <property type="entry name" value="Ribosomal_L32e_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018263">
        <property type="entry name" value="Ribosomal_L32e_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036351">
        <property type="entry name" value="Ribosomal_L32e_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23413">
        <property type="entry name" value="PTHR23413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01655">
        <property type="entry name" value="Ribosomal_L32e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01393">
        <property type="entry name" value="Ribosomal_L32e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52042">
        <property type="entry name" value="SSF52042"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00580">
        <property type="entry name" value="RIBOSOMAL_L32E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 5 6" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00015223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEF96444.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="138" mass="16357" checksum="3D358E28DB5C6410" modified="2011-07-27" version="1">MSEFKRLMRVRFKQKMKKPKFLRQEWFRYKRLGEKWRKPKGRHSGMRLQLKHRPAIVKVGYRSPKLVRGLHPSGLEDVLVHNVKELEQLDPKTQGARIAATVGKRKRIEIIKRARELGIRILNISEEKQKELLGIKGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>F6BD92</accession>
    <name>F6BD92_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ribonuclease P protein component 1</fullName>
            <shortName evidence="6">RNase P component 1</shortName>
            <ecNumber evidence="6">3.1.26.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Rpp29</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">rnp1</name>
        <name evidence="7" type="ordered locus">Metig_0910</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Part of ribonuclease P, a protein complex that generates mature tRNA molecules by cleaving their 5'-ends.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>Endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor.</text>
            <dbReference type="EC" id="3.1.26.5"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="6">Consists of a catalytic RNA component and at least 4-5 protein subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the eukaryotic/archaeal RNase P protein component 1 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.5" evidence="6"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96453.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799055.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0910"/>
    <dbReference type="EnsemblBacteria" id="AEF96453">
        <property type="protein sequence ID" value="AEF96453"/>
        <property type="gene ID" value="Metig_0910"/>
    </dbReference>
    <dbReference type="GeneID" id="10643754"/>
    <dbReference type="KEGG" id="mig:Metig_0910"/>
    <dbReference type="HOGENOM" id="CLU_107020_2_1_2"/>
    <dbReference type="OMA" id="WHELIGL"/>
    <dbReference type="OrthoDB" id="124913at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030677">
        <property type="term" value="C:ribonuclease P complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004526">
        <property type="term" value="F:ribonuclease P activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001682">
        <property type="term" value="P:tRNA 5'-leader removal"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00754">
        <property type="entry name" value="RNase_P_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036980">
        <property type="entry name" value="RNase_P/MRP_Rpp29_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023538">
        <property type="entry name" value="RNP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023534">
        <property type="entry name" value="Rof/RNase_P-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002730">
        <property type="entry name" value="Rpp29/RNP1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01868">
        <property type="entry name" value="RNase_P-MRP_p29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00538">
        <property type="entry name" value="POP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101744">
        <property type="entry name" value="SSF101744"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 6" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="5 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 6" id="KW-0540">Nuclease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0819">tRNA processing</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEF96453.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="95" mass="11029" checksum="44616C134507988C" modified="2011-07-27" version="1">MITPYNILRHELIGLEVEIVKSRNPSMIGIKGRVVDESRNTLVIEKENGKEVVIPKDIAVFRFKLPTCRVDVDGRLLIGRPEDRLKRKIKELYPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDB1</accession>
    <name>F6BDB1_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Hydrogen dehydrogenase</fullName>
            <ecNumber evidence="2">1.12.1.2</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0929</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.12.1.2" evidence="2"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96472.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799074.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0929"/>
    <dbReference type="EnsemblBacteria" id="AEF96472">
        <property type="protein sequence ID" value="AEF96472"/>
        <property type="gene ID" value="Metig_0929"/>
    </dbReference>
    <dbReference type="GeneID" id="10643775"/>
    <dbReference type="KEGG" id="mig:Metig_0929"/>
    <dbReference type="HOGENOM" id="CLU_044556_0_0_2"/>
    <dbReference type="OMA" id="QNNLAMN"/>
    <dbReference type="OrthoDB" id="32760at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008901">
        <property type="term" value="F:ferredoxin hydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047985">
        <property type="term" value="F:hydrogen dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016151">
        <property type="term" value="F:nickel cation binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.645.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001501">
        <property type="entry name" value="Ni-dep_hyd_lsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018194">
        <property type="entry name" value="Ni-dep_hyd_lsu_Ni_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029014">
        <property type="entry name" value="NiFe-Hase_large"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00374">
        <property type="entry name" value="NiFeSe_Hases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56762">
        <property type="entry name" value="SSF56762"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00507">
        <property type="entry name" value="NI_HGENASE_L_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96472.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="419" mass="46255" checksum="BD134666EB523C7E" modified="2011-07-27" version="1">MGKVVIEPLTRLEGHGKVTITLDENGKPKDVKLHITALRGFEQFVVGRPAEEVPRIVPRICGICQTAHHLASVKAVDAAWGVEIPSAAKKVRELMHLGNMIHSHALHFYFLAAPDFVVGPDADPAIRNIVGVIDKAPEVAKKAIALRKFGQALVEATGGKAIHPVTGIPGGVSKPFSEEDRDAFLKEIDTMIEYAKDGVELIKSINEQYMEQIKTLGVIDTWYLGLVNDGKHEFYDGTLRFLSPDGKEKMEFQPSEYLDYIGEHVVSHSYVKYPYNKKVGYPEGLYRVGPLAMLNVCDSMKTPLAEEARKEFLDTFGFPANQSLAYNHARLIELLAACERVKELLNDSEITSEDVKVEVEPKAGNGVGVVYAPRGVLIHNYETDDNGIVVKANMIVATTHNVPTMEKAIQQAAEVIFKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDB7</accession>
    <name>F6BDB7_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_0935</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96478.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799080.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0935"/>
    <dbReference type="EnsemblBacteria" id="AEF96478">
        <property type="protein sequence ID" value="AEF96478"/>
        <property type="gene ID" value="Metig_0935"/>
    </dbReference>
    <dbReference type="GeneID" id="10643781"/>
    <dbReference type="KEGG" id="mig:Metig_0935"/>
    <dbReference type="HOGENOM" id="CLU_1149814_0_0_2"/>
    <dbReference type="OMA" id="AFDTYGG"/>
    <dbReference type="OrthoDB" id="88910at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012021">
        <property type="entry name" value="UCP005278"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005278">
        <property type="entry name" value="UCP005278"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF96478.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="237" mass="25799" checksum="1A095691959EF9F7" modified="2011-07-27" version="1">MYYISAIGVDISSNDVSTNPKVNEKIDYEIKKELPKIGVKAALTNITGDDIVITSFVPENLIEETNKIIIDILKKHAEGFNDLNGISDNPEDAGEGISYAIAKAGSKYGDALIVSFDTYGGEDIVNEMALFVEEIGKKFGYDASSSVSNKPKKIHGVGYVGVSTDDPVVVITFKELKELPKLAGMIFGGLLGFDRVYFVRKGTPTNILPPGVIYTMSAFLNGNVIDLYDGIKRRFNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDE7</accession>
    <name>F6BDE7_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_0965</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96508.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799110.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0965"/>
    <dbReference type="EnsemblBacteria" id="AEF96508">
        <property type="protein sequence ID" value="AEF96508"/>
        <property type="gene ID" value="Metig_0965"/>
    </dbReference>
    <dbReference type="GeneID" id="10643811"/>
    <dbReference type="KEGG" id="mig:Metig_0965"/>
    <dbReference type="HOGENOM" id="CLU_1514623_0_0_2"/>
    <dbReference type="OMA" id="NRSEGWY"/>
    <dbReference type="OrthoDB" id="89695at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF96508.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="177" mass="19943" checksum="3F99C50B070D03C8" modified="2011-07-27" version="1">MIFDSDIVIAVIVLLVGLSFYAASLSEHVHSYTSAVKTNILYDKASHTLLDLVSDGTLESCIILYNNECEDIANNILRNRINFKNYELTIGNITIKEGSPTGNEVVVSSTILLNRTEGWYGIYGNETKLNITQEYFLSESAAMQYLNETYNNSTFKRAFYYFNTSKPIEVKLKVYGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDN9</accession>
    <name>F6BDN9_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Nucleic acid binding OB-fold tRNA/helicase-type</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_1061</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96600.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1061"/>
    <dbReference type="EnsemblBacteria" id="AEF96600">
        <property type="protein sequence ID" value="AEF96600"/>
        <property type="gene ID" value="Metig_1061"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_1061"/>
    <dbReference type="HOGENOM" id="CLU_065085_0_0_2"/>
    <dbReference type="OMA" id="WICPEHG"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0347">Helicase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="56"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="56"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF96600.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="363" mass="41957" checksum="268F9BE26F07B841" modified="2011-07-27" version="1">MVDLEEVRKKIMDKLKISEKELDELIDKKVKEYGGLLNKEAATLLIFKELKELKDEDGGDEEKEEEKKGKEKEEMEEEVELDEIDVDIIPLEDVLERMIEINAVARIERIFDVKEVNGNKVVNILLNDGTRRALLSLWNEDIELIKTKNLKEGDCVLIKNAYAPKIYKDRVHLSIREGDIVKVKADLPPIEELIRMVNEKKLSFERKKIDELKEGEGAEIRGIIVAIHSSEPYFPVCEKCNKKMVLKKGYAICGCGNKVDEESEDLKWIFLCNVTVDDGSGNIKVVLNDNTDAIDFNELKRLVIEGEDLEKYLNEKLLGMDVVVKGIVVFDDYLQSPVFKARYWEETNPIEEFKRKKEQHNKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDS1</accession>
    <name>F6BDS1_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_1093</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96632.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799233.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96632">
        <property type="protein sequence ID" value="AEF96632"/>
        <property type="gene ID" value="Metig_1093"/>
    </dbReference>
    <dbReference type="GeneID" id="10643948"/>
    <dbReference type="KEGG" id="mig:Metig_1093"/>
    <dbReference type="HOGENOM" id="CLU_159088_1_1_2"/>
    <dbReference type="OMA" id="DNNLMYR"/>
    <dbReference type="OrthoDB" id="124865at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019209">
        <property type="entry name" value="DUF2098"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017099">
        <property type="entry name" value="UCP037053"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09871">
        <property type="entry name" value="DUF2098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037053">
        <property type="entry name" value="UCP037053"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF96632.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="92" mass="10509" checksum="297B182BD3A40EA9" modified="2011-07-27" version="1">MIQDINGNEITIGSYVRYINTGTKGHVVDIKIDGNDTWVVLDNDLMYRPNLLEVLEEKKEEKKEELDKDELIKKLIEEGKIEVSNDIDACGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-06-02" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDS4</accession>
    <name>F6BDS4_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Resolvase domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_1096</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96635.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799236.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96635">
        <property type="protein sequence ID" value="AEF96635"/>
        <property type="gene ID" value="Metig_1096"/>
    </dbReference>
    <dbReference type="GeneID" id="10643954"/>
    <dbReference type="KEGG" id="mig:Metig_1096"/>
    <dbReference type="HOGENOM" id="CLU_101656_0_0_2"/>
    <dbReference type="OMA" id="KSVYEYM"/>
    <dbReference type="OrthoDB" id="116373at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013324">
        <property type="entry name" value="RNA_pol_sigma_r3/r4-like"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88659">
        <property type="entry name" value="SSF88659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF96635.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="181" mass="21942" checksum="3E32A257485CBDA5" modified="2011-07-27" version="1">MIEIKFSRFPRWDEIKELEKKAKNNIIIVKFPKSIYNSPKMKYKLEYMKRRLIFVEVDEQKRGRPKKIDESIKNHVVQLLINGKNLSEIARELNFPRTTIFDNIKDALPKIKREKFMKLLYEYKEFLIENGLYTPHVQILFSELEMHIKKEDFENAKKILDEIIKISKSARKIREKRSRKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-09-29" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDY0</accession>
    <name>F6BDY0_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="5">DNA binding domain protein, excisionase family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Metig_1152</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96691.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799292.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96691">
        <property type="protein sequence ID" value="AEF96691"/>
        <property type="gene ID" value="Metig_1152"/>
    </dbReference>
    <dbReference type="GeneID" id="10644010"/>
    <dbReference type="KEGG" id="mig:Metig_1152"/>
    <dbReference type="HOGENOM" id="CLU_082093_0_1_2"/>
    <dbReference type="OMA" id="MVINQEV"/>
    <dbReference type="OrthoDB" id="78802at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000150">
        <property type="term" value="F:DNA strand exchange activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03769">
        <property type="entry name" value="SR_IS607_transposase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1390">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009061">
        <property type="entry name" value="DNA-bd_dom_put_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041657">
        <property type="entry name" value="HTH_17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041718">
        <property type="entry name" value="IS607_transposase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006118">
        <property type="entry name" value="Recombinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006119">
        <property type="entry name" value="Resolv_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036162">
        <property type="entry name" value="Resolvase-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010093">
        <property type="entry name" value="SinI_DNA-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12728">
        <property type="entry name" value="HTH_17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00239">
        <property type="entry name" value="Resolvase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00857">
        <property type="entry name" value="Resolvase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46955">
        <property type="entry name" value="SSF46955"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53041">
        <property type="entry name" value="SSF53041"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01764">
        <property type="entry name" value="excise"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00397">
        <property type="entry name" value="RECOMBINASES_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51736">
        <property type="entry name" value="RECOMBINASES_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0229">DNA integration</keyword>
    <keyword evidence="3" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Resolvase/invertase-type recombinase catalytic" evidence="4">
        <location>
            <begin position="62"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEF96691.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="209" mass="24360" checksum="7E84B77016269FFC" modified="2011-07-27" version="1">MSLERLYTMKEACELLGIHIKTLQRWDREGKIKCVRTVGGKRRVPESEIKRILGIKDKEQRKIIGYARVSSNTQKDDLERQIDAIKSYAKDRGWNIEILKDVGSGLSEKRKNYRKLLKMVINQEVEKVIIAYPDRLTRFGFETLKEFFKSYGTEVIVINKKCKAPQEELVEDLITVISHFAGKLYGRRSHKYKKLIKTVKEIVGENNAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDY8</accession>
    <name>F6BDY8_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_1160</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96699.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799300.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1160"/>
    <dbReference type="EnsemblBacteria" id="AEF96699">
        <property type="protein sequence ID" value="AEF96699"/>
        <property type="gene ID" value="Metig_1160"/>
    </dbReference>
    <dbReference type="GeneID" id="10644020"/>
    <dbReference type="KEGG" id="mig:Metig_1160"/>
    <dbReference type="HOGENOM" id="CLU_110109_0_0_2"/>
    <dbReference type="OMA" id="IQHYPCR"/>
    <dbReference type="OrthoDB" id="56837at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003745">
        <property type="entry name" value="DUF166"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02593">
        <property type="entry name" value="DUF166"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF96699.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="220" mass="25282" checksum="2AC06954FCCC5127" modified="2011-07-27" version="1">MKIGILSEGKYGERAYNVIGKKFNCDFIKVKYSGEFDDIEIDKDVLEKLKDYDLFITYTTNPDLTYELVRQISKINKNAFVIVGAWKGGGFKKQLESFGNAFCPDLMCEIDEDVLKNYIDKFPQLKGFLKYFGRPKVKIKCIDDKIKDIEILREAPCGSTSETLKEFIGKEFNENTLKNIGLRVQHFCRASKIRLFVDNECKKIKAGEIIVQSVKEGINQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>F6BE12</accession>
    <name>F6BE12_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Peptidase M48 Ste24p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Metig_1185</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96723.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799322.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1185"/>
    <dbReference type="EnsemblBacteria" id="AEF96723">
        <property type="protein sequence ID" value="AEF96723"/>
        <property type="gene ID" value="Metig_1185"/>
    </dbReference>
    <dbReference type="GeneID" id="10644045"/>
    <dbReference type="KEGG" id="mig:Metig_1185"/>
    <dbReference type="HOGENOM" id="CLU_038032_0_0_2"/>
    <dbReference type="OMA" id="PINIAFW"/>
    <dbReference type="OrthoDB" id="111469at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001915">
        <property type="entry name" value="Peptidase_M48"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01435">
        <property type="entry name" value="Peptidase_M48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="121"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="165"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="206"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="241"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="283"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="393"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="423"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_M48" evidence="8">
        <location>
            <begin position="317"/>
            <end position="498"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AEF96723.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="525" mass="60395" checksum="06FAC6E4E02030BD" modified="2011-07-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F6BE30</accession>
    <name>F6BE30_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_0004</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95566.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798176.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0004"/>
    <dbReference type="EnsemblBacteria" id="AEF95566">
        <property type="protein sequence ID" value="AEF95566"/>
        <property type="gene ID" value="Metig_0004"/>
    </dbReference>
    <dbReference type="GeneID" id="10642839"/>
    <dbReference type="KEGG" id="mig:Metig_0004"/>
    <dbReference type="HOGENOM" id="CLU_113977_0_0_2"/>
    <dbReference type="OMA" id="FIIEHFD"/>
    <dbReference type="OrthoDB" id="71402at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007162">
        <property type="entry name" value="DUF366"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04017">
        <property type="entry name" value="DUF366"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006503">
        <property type="entry name" value="UCP006503"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="SSF55681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF95566.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="201" mass="22896" checksum="B18E159DA3C8D038" modified="2011-07-27" version="1">MHAEILDVGFMNVVFPKGRIKYTGKEIEPLWAFNMFDVQKDSIVAFRGGMEVKIENMKDLKDVKEEGEFKIPIKGDDCIHFIVEHFDNPDLKMIYLRQRILICIAKDTIESFGVKLDREGDDLYCNGKKLSVCIATRGVSSAKIHLGINVNSKGIPEHVEGIGLEDIGIFDIEEVMRKIAISYAKEMEKIEKDIRKTAPLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-06-02" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>F6BE58</accession>
    <name>F6BE58_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0033</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95594.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798203.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF95594">
        <property type="protein sequence ID" value="AEF95594"/>
        <property type="gene ID" value="Metig_0033"/>
    </dbReference>
    <dbReference type="GeneID" id="10642868"/>
    <dbReference type="KEGG" id="mig:Metig_0033"/>
    <dbReference type="HOGENOM" id="CLU_1582909_0_0_2"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="145"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF95594.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="168" mass="18612" checksum="82F83298E0FBD6FA" modified="2011-07-27" version="1">MNKTIFLILCLFGSIVCIYGESYSDTVSVNSGWTADRISWTHELPSNYTNITSLTLIIEAYDVNFKNVVTINDITVGNLNKTGTNQWGTTTLTLDANTLQQISNAHPSSLRVTVTPYCDWIKINTSTLTMEYVVDNGQGNSSVKAPIPLFSIILSIVFMSSIIYNKIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>F6BE84</accession>
    <name>F6BE84_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">30S ribosomal protein S19P</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">rps19p</name>
        <name evidence="7" type="ordered locus">Metig_1224</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 5">Protein S19 forms a complex with S13 that binds strongly to the 16S ribosomal RNA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 5 6">Belongs to the universal ribosomal protein uS19 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96761.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799360.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1224"/>
    <dbReference type="EnsemblBacteria" id="AEF96761">
        <property type="protein sequence ID" value="AEF96761"/>
        <property type="gene ID" value="Metig_1224"/>
    </dbReference>
    <dbReference type="GeneID" id="10644085"/>
    <dbReference type="KEGG" id="mig:Metig_1224"/>
    <dbReference type="HOGENOM" id="CLU_097347_1_1_2"/>
    <dbReference type="OMA" id="VIRTHCR"/>
    <dbReference type="OrthoDB" id="99236at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.860.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00531">
        <property type="entry name" value="Ribosomal_S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020934">
        <property type="entry name" value="Ribosomal_S15/S19_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002222">
        <property type="entry name" value="Ribosomal_S19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023575">
        <property type="entry name" value="Ribosomal_S19_S15_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005713">
        <property type="entry name" value="Ribosomal_S19A/S15e"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11880">
        <property type="entry name" value="PTHR11880"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00203">
        <property type="entry name" value="Ribosomal_S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002144">
        <property type="entry name" value="Ribosomal_S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00975">
        <property type="entry name" value="RIBOSOMALS19"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54570">
        <property type="entry name" value="SSF54570"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01025">
        <property type="entry name" value="uS19_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00323">
        <property type="entry name" value="RIBOSOMAL_S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 5 6" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0699">rRNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEF96761.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="152" mass="17702" checksum="A8990CDC1228C817" modified="2011-07-27" version="1">MARGRAARKKKKQQVTVRRGEFRYRGYTLEELQQMPLKEFIKLLPARQRRSMLRGLTPQQRKLAMKIKKARRLLKKGKEPRIIRTHCRDFIITPDMVGLTFGVYNGKEFVEVKVTEEMIGHYLGEFSLTRKPVQHGSPGMGATRSSMFVPIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>F6BE85</accession>
    <name>F6BE85_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Hydro-lyase, Fe-S type, tartrate/fumarate subfamily, beta subunit</fullName>
            <ecNumber evidence="4">4.2.1.2</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metig_1225</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-I fumarase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.2" evidence="4"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96762.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799361.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1225"/>
    <dbReference type="EnsemblBacteria" id="AEF96762">
        <property type="protein sequence ID" value="AEF96762"/>
        <property type="gene ID" value="Metig_1225"/>
    </dbReference>
    <dbReference type="GeneID" id="10644086"/>
    <dbReference type="KEGG" id="mig:Metig_1225"/>
    <dbReference type="HOGENOM" id="CLU_098588_0_0_2"/>
    <dbReference type="OMA" id="IYHCGPL"/>
    <dbReference type="OrthoDB" id="88282at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004333">
        <property type="term" value="F:fumarate hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.130.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004647">
        <property type="entry name" value="Fe-S_hydro-lyase_TtdB-typ_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036660">
        <property type="entry name" value="Fe-S_hydroAse_TtdB_cat_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05683">
        <property type="entry name" value="Fumerase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117457">
        <property type="entry name" value="SSF117457"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00723">
        <property type="entry name" value="ttdB_fumA_fumB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0456">Lyase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fumerase_C" evidence="3">
        <location>
            <begin position="5"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF05683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEF96762.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="199" mass="22215" checksum="2BE6E3CEEF112688" modified="2011-07-27" version="1">MASFELKTPISKETISKLKVGDVVYLNGVIYTARDEAHLEIIKYHKKGKELPFNLENGVIYHAGPIMKKENDKWVCVAIGPTTSARMNNTEEEVIKVTNLSAIVGKGGMKKELLKTFKEHKVVYLAAPGGCAALLANCIKEVKNVYFLELGMPEAVWELEVENFGPLIVAMDAHGNSIYEEVNEKVFENLKMMVQSQKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>F6BE96</accession>
    <name>F6BE96_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Methyl-coenzyme M reductase subunit gamma</fullName>
            <ecNumber evidence="8">2.8.4.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Metig_1236</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Component of the methyl-coenzyme M reductase (MCR) I that catalyzes the reductive cleavage of methyl-coenzyme M (CoM-S-CH3 or 2-(methylthio)ethanesulfonate) using coenzyme B (CoB or 7-mercaptoheptanoylthreonine phosphate) as reductant which results in the production of methane and the mixed heterodisulfide of CoB and CoM (CoM-S-S-CoB). This is the final step in methanogenesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>coenzyme B + methyl-coenzyme M = coenzyme M-coenzyme B heterodisulfide + methane</text>
            <dbReference type="Rhea" id="RHEA:12532"/>
            <dbReference type="ChEBI" id="CHEBI:16183"/>
            <dbReference type="ChEBI" id="CHEBI:58286"/>
            <dbReference type="ChEBI" id="CHEBI:58411"/>
            <dbReference type="ChEBI" id="CHEBI:58596"/>
            <dbReference type="EC" id="2.8.4.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="1">
            <dbReference type="Rhea" id="RHEA:12533"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>coenzyme F430</name>
            <dbReference type="ChEBI" id="CHEBI:60540"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4 8">One-carbon metabolism; methyl-coenzyme M reduction; methane from methyl-coenzyme M: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Hexamer of two alpha, two beta, and two gamma chains.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">MCR is a hexamer of two alpha, two beta, and two gamma chains, forming a dimer of heterotrimers.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the methyl-coenzyme M reductase gamma subunit family.</text>
    </comment>
    <dbReference type="EC" id="2.8.4.1" evidence="8"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96773.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799372.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1236"/>
    <dbReference type="EnsemblBacteria" id="AEF96773">
        <property type="protein sequence ID" value="AEF96773"/>
        <property type="gene ID" value="Metig_1236"/>
    </dbReference>
    <dbReference type="GeneID" id="10644099"/>
    <dbReference type="KEGG" id="mig:Metig_1236"/>
    <dbReference type="HOGENOM" id="CLU_1092436_0_0_2"/>
    <dbReference type="OMA" id="IMGHRQP"/>
    <dbReference type="OrthoDB" id="51032at2157"/>
    <dbReference type="UniPathway" id="UPA00646">
        <property type="reaction ID" value="UER00699"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050524">
        <property type="term" value="F:coenzyme-B sulfoethylthiotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015948">
        <property type="term" value="P:methanogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00539">
        <property type="entry name" value="MCR_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.320.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009024">
        <property type="entry name" value="Me_CoM_Rdtase_Fd-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003178">
        <property type="entry name" value="Me_CoM_Rdtase_gsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036994">
        <property type="entry name" value="Me_CoM_Rdtase_gsu_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02240">
        <property type="entry name" value="MCR_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000264">
        <property type="entry name" value="Meth_CoM_rd_gama"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55088">
        <property type="entry name" value="SSF55088"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03259">
        <property type="entry name" value="met_CoM_red_gam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0484">Methanogenesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 9" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000951"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00002461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00008740"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00011155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR000264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AEF96773.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="260" mass="30211" checksum="41F74C3D17432397" modified="2011-07-27" version="1">MAYKPQFYPGETKIAQNRRNHMNPDVELEKLREIPDEDVVKIMGHRQPGEDYKTIHPPLEEMDLPEDYVRDLVEPINGAKEGHRIRYIQFADSMYFAPAQPYDRARTYMWRFRGVDTGTLSGRQVIEMRESDLEALSKNFLIDTAFFDPARCGIRGATVHGHSLRLDENGLMFDALQRYVYDEKTGHVVYVKDQVGRPLDEPVDVGEPLPEEKLREITTIYRKDGIPMREDKELLTVVKRIHRARTLGGFCPTEDVFKEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEB1</accession>
    <name>F6BEB1_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CRISPR system Cms protein Csm5</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">CRISPR type III A-associated protein Csm5</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Metig_1251</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">This subunit might be involved in maturation of a crRNA intermediate to its mature form.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CRISPR-associated Csm5 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96788.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799387.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1251"/>
    <dbReference type="EnsemblBacteria" id="AEF96788">
        <property type="protein sequence ID" value="AEF96788"/>
        <property type="gene ID" value="Metig_1251"/>
    </dbReference>
    <dbReference type="GeneID" id="10644114"/>
    <dbReference type="KEGG" id="mig:Metig_1251"/>
    <dbReference type="HOGENOM" id="CLU_036878_3_0_2"/>
    <dbReference type="OMA" id="PIFIGCG"/>
    <dbReference type="OrthoDB" id="41800at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010173">
        <property type="entry name" value="CRISPR-assoc_Csm5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005537">
        <property type="entry name" value="RAMP_III_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38007">
        <property type="entry name" value="PTHR38007"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03787">
        <property type="entry name" value="RAMPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01899">
        <property type="entry name" value="cas_TM1807_csm5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0051">Antiviral defense</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0694">RNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00031720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEF96788.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="380" mass="45047" checksum="F8B9AFC59003755A" modified="2011-07-27" version="1">MMLKCSLITPIFIGCGEEYTPLDYYIENNIAHIVDIEKALEDLGDLSNIDKISNYIVNKSIGNRVETNAKDILERVGLCAEDYVVYKIKSEIKEDSRTRVKKFIHQNNRKYVPGSSIKGAIRTAYLFNYYDGKISEIFKILDNKKIDDKGKEIERRAIGDIRDDFFKYFKISDSIKLEREEFKFILTKRWHIKKKKLTIPNYLEGMTKGEFTINLKVEEEFFKELNNRLNKNFNPKNNHEKFEIIKELCNNFSKTVVDFEIKRDLPILLKEFYEKLKKDIEKNDALYINLGFEGGFLNKTIYPLLWKNDQNNVLFHKIRQFFLDLYKPKGKINPRNPQYKLYQTWQNAKSYLDFPTTVGVYVRDNRIISPLGWIKLEKVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEG2</accession>
    <name>F6BEG2_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Glutamate--tRNA ligase</fullName>
            <ecNumber evidence="7">6.1.1.17</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Glutamyl-tRNA synthetase</fullName>
            <shortName evidence="7">GluRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">gltX</name>
        <name evidence="11" type="ordered locus">Metig_0062</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu)</text>
            <dbReference type="Rhea" id="RHEA:23540"/>
            <dbReference type="Rhea" id="RHEA-COMP:9663"/>
            <dbReference type="Rhea" id="RHEA-COMP:9680"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78520"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.17"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the class-I aminoacyl-tRNA synthetase family. Glutamate--tRNA ligase type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.17" evidence="7"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95623.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798232.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0062"/>
    <dbReference type="EnsemblBacteria" id="AEF95623">
        <property type="protein sequence ID" value="AEF95623"/>
        <property type="gene ID" value="Metig_0062"/>
    </dbReference>
    <dbReference type="GeneID" id="10642897"/>
    <dbReference type="KEGG" id="mig:Metig_0062"/>
    <dbReference type="HOGENOM" id="CLU_001882_1_3_2"/>
    <dbReference type="OMA" id="MRFAPNP"/>
    <dbReference type="OrthoDB" id="8922at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004818">
        <property type="term" value="F:glutamate-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006424">
        <property type="term" value="P:glutamyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.240.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02076">
        <property type="entry name" value="Glu_tRNA_synth_type2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004526">
        <property type="entry name" value="Glu-tRNA-synth_arc/euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000924">
        <property type="entry name" value="Glu/Gln-tRNA-synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020058">
        <property type="entry name" value="Glu/Gln-tRNA-synth_Ib_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020059">
        <property type="entry name" value="Glu/Gln-tRNA-synth_Ib_codon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020056">
        <property type="entry name" value="Rbsml_L25/Gln-tRNA_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011035">
        <property type="entry name" value="Ribosomal_L25/Gln-tRNA_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00749">
        <property type="entry name" value="tRNA-synt_1c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03950">
        <property type="entry name" value="tRNA-synt_1c_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00987">
        <property type="entry name" value="TRNASYNTHGLU"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50715">
        <property type="entry name" value="SSF50715"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00463">
        <property type="entry name" value="gltX_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7 8" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="4 7 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 7 8" id="KW-0436">Ligase</keyword>
    <keyword evidence="3 7 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 7 8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="tRNA-synt_1c" evidence="9">
        <location>
            <begin position="95"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="domain" description="tRNA-synt_1c_C" evidence="10">
        <location>
            <begin position="400"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'HIGH' region" evidence="7">
        <location>
            <begin position="102"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001818"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF03950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AEF95623.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="557" mass="65020" checksum="7AC9CA9C63F2D084" modified="2011-07-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEI1</accession>
    <name>F6BEI1_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Nitrogen regulatory protein P-II</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_0081</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the P(II) protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95642.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798251.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0081"/>
    <dbReference type="EnsemblBacteria" id="AEF95642">
        <property type="protein sequence ID" value="AEF95642"/>
        <property type="gene ID" value="Metig_0081"/>
    </dbReference>
    <dbReference type="GeneID" id="10642916"/>
    <dbReference type="KEGG" id="mig:Metig_0081"/>
    <dbReference type="HOGENOM" id="CLU_082268_0_0_2"/>
    <dbReference type="OMA" id="DLAPQIV"/>
    <dbReference type="OrthoDB" id="113533at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030234">
        <property type="term" value="F:enzyme regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006808">
        <property type="term" value="P:regulation of nitrogen utilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002187">
        <property type="entry name" value="N-reg_PII"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011322">
        <property type="entry name" value="N-reg_PII-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015867">
        <property type="entry name" value="N-reg_PII/ATP_PRibTrfase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017918">
        <property type="entry name" value="N-reg_PII_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30115">
        <property type="entry name" value="PTHR30115"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00543">
        <property type="entry name" value="P-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039144">
        <property type="entry name" value="GlnB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00340">
        <property type="entry name" value="PIIGLNB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00938">
        <property type="entry name" value="P-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54913">
        <property type="entry name" value="SSF54913"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00638">
        <property type="entry name" value="PII_GLNB_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51343">
        <property type="entry name" value="PII_GLNB_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="modified residue" description="O-UMP-tyrosine" evidence="1">
        <location>
            <position position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR602187-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF95642.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="112" mass="12340" checksum="F3ABAC3A014D157D" modified="2011-07-27" version="1">MKKIEAIIRPSKLEDVKSALFKAGCKGLTVSEVKGRGVQGGIVERYRGREYVVDLLPKVKIEIVVDDANVEKIIDIICENAKTGEFGDGKIFVIPVEEVVRVRTGERGENAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEP9</accession>
    <name>F6BEP9_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0273 protein Metig_1309</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_1309</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0273 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96846.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799443.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1309"/>
    <dbReference type="EnsemblBacteria" id="AEF96846">
        <property type="protein sequence ID" value="AEF96846"/>
        <property type="gene ID" value="Metig_1309"/>
    </dbReference>
    <dbReference type="GeneID" id="10644172"/>
    <dbReference type="KEGG" id="mig:Metig_1309"/>
    <dbReference type="HOGENOM" id="CLU_023669_2_0_2"/>
    <dbReference type="OMA" id="FSQQFLW"/>
    <dbReference type="OrthoDB" id="76011at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01076">
        <property type="entry name" value="UPF0273"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014774">
        <property type="entry name" value="KaiC-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010624">
        <property type="entry name" value="KaiC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022475">
        <property type="entry name" value="UPF0273_KaiC-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06745">
        <property type="entry name" value="ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03877">
        <property type="entry name" value="thermo_KaiC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51146">
        <property type="entry name" value="KAIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="KaiC" evidence="2">
        <location>
            <begin position="2"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="29"/>
            <end position="36"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF96846.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="241" mass="26793" checksum="091720488E59D57B" modified="2011-07-27" version="1">MHRVKTGIPGMDEILHGGIPERNVVLLSGGPGTGKSIFCQQFLFKGIEEYDEPGILIALEEHPVQIRINMEQFGWDVRKHEKEGKFAIVDAFTSGIGSAAKREKYIVRDPNDEMELIDVMKEAIEEIGAKRVGIDSVTTLYLNKPMMARKTVFLLKKVLAGLGCTALFVSQISVGERGFGGPGVEHAVDGIIKLDLDEIDGELKRSLIVWKMRGTSHSMRRHPFEITNKGIIVHSDKVLKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEQ1</accession>
    <name>F6BEQ1_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Radical SAM domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_1311</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96848.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1311"/>
    <dbReference type="EnsemblBacteria" id="AEF96848">
        <property type="protein sequence ID" value="AEF96848"/>
        <property type="gene ID" value="Metig_1311"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_1311"/>
    <dbReference type="HOGENOM" id="CLU_042889_0_0_2"/>
    <dbReference type="OMA" id="AHYFMPL"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031419">
        <property type="term" value="F:cobalamin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.30.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023980">
        <property type="entry name" value="CHP04013_B12-bd/rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006158">
        <property type="entry name" value="Cobalamin-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaB/NifB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023404">
        <property type="entry name" value="rSAM_horseshoe"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02310">
        <property type="entry name" value="B12-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="SSF51366"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR04013">
        <property type="entry name" value="B12_SAM_MJ_1487"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Radical SAM core" evidence="2">
        <location>
            <begin position="147"/>
            <end position="374"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF96848.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="387" mass="44583" checksum="9B18B9D46F2AB0F7" modified="2011-07-27" version="1">MCPISNANDHNLNIGFRLTSKNRYSVSKLYPLIGGKLFKDFNKLISNIEDLDILIYSFMTMQRDNVLEEINKIIQIKKEKELEKPILIAGGPHPSGNPKDAINMGFDYVIVGEGEITLPKLINKLKKEKIEERIIIGERVKNLDEYNEIYPMTPIEITRGCPFNCRFCQTPQIFGKEVRHRSIENIVKLVKNMGDLRFITPNAFCYGSKDGLKPNIEKLERLLRELSKVKGRLFFGTFPSEVRPEFIKKETIELVVEYCDNKYLHFGAQSGSDEMLRYIRRGHTVDDVLNAIDLCVEYGLIPKVDFIFGFPNENEHHRKESIELIKYIIKKNGKVHAHYFMPLPGTYFENSSPTPLDKETLKILGKLAQKGLISGSWGYQYMKNVNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>F6BER0</accession>
    <name>F6BER0_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">30S ribosomal protein S17e</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">rps17e</name>
        <name evidence="5" type="ordered locus">Metig_1320</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein eS17 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96857.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799454.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1320"/>
    <dbReference type="EnsemblBacteria" id="AEF96857">
        <property type="protein sequence ID" value="AEF96857"/>
        <property type="gene ID" value="Metig_1320"/>
    </dbReference>
    <dbReference type="GeneID" id="10644183"/>
    <dbReference type="KEGG" id="mig:Metig_1320"/>
    <dbReference type="HOGENOM" id="CLU_176720_0_1_2"/>
    <dbReference type="OMA" id="TRDFEHN"/>
    <dbReference type="OrthoDB" id="128227at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.60.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00511">
        <property type="entry name" value="Ribosomal_S17e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001210">
        <property type="entry name" value="Ribosomal_S17e"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018273">
        <property type="entry name" value="Ribosomal_S17e_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036401">
        <property type="entry name" value="RPS17e-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10732">
        <property type="entry name" value="PTHR10732"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00833">
        <property type="entry name" value="Ribosomal_S17e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF116820">
        <property type="entry name" value="SSF116820"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00712">
        <property type="entry name" value="RIBOSOMAL_S17E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 5" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEF96857.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="63" mass="7533" checksum="6C0D572B63094916" modified="2011-07-27" version="1">MGRIRQSFIKRIGDELIEKYKDKFTTDFETNKKIVQEVALISTKRLRNRIAGYITHKMKQMSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-06-02" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>F6BES4</accession>
    <name>F6BES4_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_1334</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96871.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799468.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96871">
        <property type="protein sequence ID" value="AEF96871"/>
        <property type="gene ID" value="Metig_1334"/>
    </dbReference>
    <dbReference type="GeneID" id="10644197"/>
    <dbReference type="KEGG" id="mig:Metig_1334"/>
    <dbReference type="HOGENOM" id="CLU_182714_0_0_2"/>
    <dbReference type="OMA" id="KRIDAKM"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="68"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF96871.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="93" mass="11269" checksum="6D6B844E121DAB7A" modified="2011-07-27" version="1">MDDIEEKLELLSKKMVELEKRIDAKMKESEKKVDKKIQEFSKQNSMFLVAMYIKMLNDIAKELKRIKYFLLLLNLVFYLAVFGIFMYFVFNLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>F6BET6</accession>
    <name>F6BET6_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 9">Adenylosuccinate lyase</fullName>
            <shortName evidence="9">ASL</shortName>
            <ecNumber evidence="5 9">4.3.2.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8 9">Adenylosuccinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">Metig_1348</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>(2S)-2-[5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamido]succinate = 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + fumarate</text>
            <dbReference type="Rhea" id="RHEA:23920"/>
            <dbReference type="ChEBI" id="CHEBI:29806"/>
            <dbReference type="ChEBI" id="CHEBI:58443"/>
            <dbReference type="ChEBI" id="CHEBI:58475"/>
            <dbReference type="EC" id="4.3.2.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>N(6)-(1,2-dicarboxyethyl)-AMP = AMP + fumarate</text>
            <dbReference type="Rhea" id="RHEA:16853"/>
            <dbReference type="ChEBI" id="CHEBI:29806"/>
            <dbReference type="ChEBI" id="CHEBI:57567"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="4.3.2.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 9">Purine metabolism; AMP biosynthesis via de novo pathway; AMP from IMP: step 2/2.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 9">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate: step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homotetramer. Residues from neighboring subunits contribute catalytic and substrate-binding residues to each active site.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.3.2.2" evidence="5 9"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96883.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799480.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1348"/>
    <dbReference type="EnsemblBacteria" id="AEF96883">
        <property type="protein sequence ID" value="AEF96883"/>
        <property type="gene ID" value="Metig_1348"/>
    </dbReference>
    <dbReference type="GeneID" id="10644211"/>
    <dbReference type="KEGG" id="mig:Metig_1348"/>
    <dbReference type="HOGENOM" id="CLU_030949_0_1_2"/>
    <dbReference type="OMA" id="ASSCEKI"/>
    <dbReference type="OrthoDB" id="39322at2157"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00132"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00075">
        <property type="reaction ID" value="UER00336"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070626">
        <property type="term" value="F:(S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido) succinate lyase (fumarate-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004018">
        <property type="term" value="F:N6-(1,2-dicarboxyethyl)AMP AMP-lyase (fumarate-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044208">
        <property type="term" value="P:'de novo' AMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.275.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019468">
        <property type="entry name" value="AdenyloSucc_lyase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024083">
        <property type="entry name" value="Fumarase/histidase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020557">
        <property type="entry name" value="Fumarate_lyase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000362">
        <property type="entry name" value="Fumarate_lyase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022761">
        <property type="entry name" value="Fumarate_lyase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008948">
        <property type="entry name" value="L-Aspartase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004769">
        <property type="entry name" value="Pur_lyase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10397">
        <property type="entry name" value="ADSL_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00206">
        <property type="entry name" value="Lyase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00149">
        <property type="entry name" value="FUMRATELYASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00998">
        <property type="entry name" value="ADSL_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48557">
        <property type="entry name" value="SSF48557"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00928">
        <property type="entry name" value="purB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00163">
        <property type="entry name" value="FUMARATE_LYASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9 12" id="KW-0456">Lyase</keyword>
    <keyword evidence="7 9" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ADSL_C" evidence="11">
        <location>
            <begin position="362"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="21"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="112"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00017058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00030717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AEF96883.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="448" mass="50962" checksum="F6BFEEC57125050A" modified="2011-07-27" version="1">MAVHPIDYRYGTEEMKKVWEEENKLQKMLEVEAALAKAEAELGIIPKEAAEEINRKASTKYVKLERVKEIERETKHDVVAMVKAFAEVCEGNAGEYIHFGATSNDIVDTAQSLQFKEAIAILERKLKELRDILLEKAEEHKYTVCIGRTHGQHAIPTTYGMRFALWASEIQRHIERLEQCKERICVSMITGAVGTMAALGEKGLEVHKRVGEILGLNPVLISNQVIQRDRHAEFIALLALIAQTLNKIGVTVRSMQRTEIGELEEEFDPTKQTGSSTMPHKRNPITFEQICGLSRVVKSLALAEFDNIPLWEERDLTNSSAERCIFAEACVLLDHILTLAVKGIKKLRVNIENVEKNLELTKGLIMAERIMIELAKRGMGRQTAHEVVRKCAMKAYDEKRHLKEVLLENEEVMKYLTKEELEELMNPKTYIGLAPQIVDEVIRELKNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEU3</accession>
    <name>F6BEU3_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="4">rRNA (Guanine-N(2)-)-methyltransferase</fullName>
            <ecNumber evidence="4">2.1.1.171</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metig_0118</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.1.1.171" evidence="4"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95679.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798288.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0118"/>
    <dbReference type="EnsemblBacteria" id="AEF95679">
        <property type="protein sequence ID" value="AEF95679"/>
        <property type="gene ID" value="Metig_0118"/>
    </dbReference>
    <dbReference type="GeneID" id="10642953"/>
    <dbReference type="KEGG" id="mig:Metig_0118"/>
    <dbReference type="HOGENOM" id="CLU_032119_0_0_2"/>
    <dbReference type="OMA" id="GFMYKAN"/>
    <dbReference type="OrthoDB" id="47556at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052913">
        <property type="term" value="F:16S rRNA (guanine(966)-N(2))-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006400">
        <property type="term" value="P:tRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002052">
        <property type="entry name" value="DNA_methylase_N6_adenine_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000241">
        <property type="entry name" value="RNA_methylase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004114">
        <property type="entry name" value="THUMP_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02926">
        <property type="entry name" value="THUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01170">
        <property type="entry name" value="UPF0020"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00981">
        <property type="entry name" value="THUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00092">
        <property type="entry name" value="N6_MTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51165">
        <property type="entry name" value="THUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="THUMP" evidence="3">
        <location>
            <begin position="45"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEF95679.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="384" mass="43748" checksum="7FC092036826C4DA" modified="2011-07-27" version="1">MKFKYYATLPPGLEKISSDEIEELGGRIFRIKEGKGRIFFEGDFELIPKVNYLSRTIERTIILLKLEEFENITLDDIYKSVYEIDWTEWIREEQAFAIRPLRAGEHDFTSIDVGRVAGQAVIDAYKNAKGVRLKVNLDNPDVIIRVDVIFNELIIGIDTTGDEGLHRRGYRAYNHPAHLNASIASALVKLSNWKDDEILLDPMCGSGTILVEAAMIKRNIPPGKFRDDFAFIKIFGREALDEIKSQIVENKKMLKLYGVEKFKKHISGAVKNAENVGVVDTIKFIEGDATELEKIDYLKDGVDVVITNPPYGIRIGGKKMVRNLYNNFLNSLKKITHDDSRIIIITAEDKILRNAAINNNYKIKEEFRVMYGGLDTVVFVLKNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEU6</accession>
    <name>F6BEU6_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 9">Threonine synthase</fullName>
            <ecNumber evidence="5 9">4.2.3.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">Metig_0121</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the gamma-elimination of phosphate from L-phosphohomoserine and the beta-addition of water to produce L-threonine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>H2O + O-phospho-L-homoserine = L-threonine + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10840"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57590"/>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
            <dbReference type="EC" id="4.2.3.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 9 10">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 9">Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 5/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 9">Belongs to the threonine synthase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.3.1" evidence="5 9"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95682.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798291.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0121"/>
    <dbReference type="EnsemblBacteria" id="AEF95682">
        <property type="protein sequence ID" value="AEF95682"/>
        <property type="gene ID" value="Metig_0121"/>
    </dbReference>
    <dbReference type="GeneID" id="10642956"/>
    <dbReference type="KEGG" id="mig:Metig_0121"/>
    <dbReference type="HOGENOM" id="CLU_028142_4_1_2"/>
    <dbReference type="OMA" id="MWGFQAS"/>
    <dbReference type="OrthoDB" id="16594at2157"/>
    <dbReference type="UniPathway" id="UPA00050">
        <property type="reaction ID" value="UER00065"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004795">
        <property type="term" value="F:threonine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009088">
        <property type="term" value="P:threonine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="PLP-dep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000634">
        <property type="entry name" value="Ser/Thr_deHydtase_PyrdxlP-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004450">
        <property type="entry name" value="Thr_synthase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026260">
        <property type="entry name" value="Thr_Synthase_bac/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="Trypto_synt_PLP_dependent"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00291">
        <property type="entry name" value="PALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038945">
        <property type="entry name" value="Thr_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="SSF53686"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00260">
        <property type="entry name" value="thrC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00165">
        <property type="entry name" value="DEHYDRATASE_SER_THR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="9 13" id="KW-0456">Lyase</keyword>
    <keyword evidence="8 9 10" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 9" id="KW-0791">Threonine biosynthesis</keyword>
    <feature type="domain" description="PALP" evidence="12">
        <location>
            <begin position="68"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="region of interest" description="Pyridoxal phosphate binding" evidence="10">
        <location>
            <begin position="233"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="132"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="371"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="11">
        <location>
            <position position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00018679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR038945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR038945-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR038945-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AEF95682.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="405" mass="44376" checksum="2764002CC966D806" modified="2011-07-27" version="1">MIQKCIRCGKEYDFDEIIYTCECGGLLEIVYDYEAIKEKVSKETLRKREIGVWRYLEYMPVKDPKKIVTLHEGGTPLYKCDNLAKELGLKELYVKNEGANPTGSFKDRGMTVGVTRANELGVKVVGCASTGNTSASLAAYSARSGKKCIVLLPEGKVALGKLAQAMFYGAKVIQIKGNFDDALEMVRELAEKKLLYLLNSVNPFRLEGQKTIGFEICDQLNWEVPDRVILPVGNAGNISAIWKGFKEFRETGIIDELPKMTGIQAEGAKPIVDAVKKGAKEIIPEEHPETIATAIRIGNPVNAPKALDAIYSSGGTAESVTDEEIIHAQKLLARTEGIFVEPASASSIAGLIKLLDMGVIDRDERIVCITTGHGLKDPDAAIRASEKPIEIECDIKVLEKLLMEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEX0</accession>
    <name>F6BEX0_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="3">AAA ATPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_0146</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the archaeal ATPase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95706.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798315.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF95706">
        <property type="protein sequence ID" value="AEF95706"/>
        <property type="gene ID" value="Metig_0146"/>
    </dbReference>
    <dbReference type="GeneID" id="10642981"/>
    <dbReference type="KEGG" id="mig:Metig_0146"/>
    <dbReference type="HOGENOM" id="CLU_068608_0_0_2"/>
    <dbReference type="OMA" id="WYAIREI"/>
    <dbReference type="OrthoDB" id="40714at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011579">
        <property type="entry name" value="ATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01637">
        <property type="entry name" value="ATPase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA" evidence="2">
        <location>
            <begin position="21"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF95706.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="335" mass="39914" checksum="D8C6FD3DE58F041F" modified="2011-07-27" version="1">MKFFNREKELNYLKTYCQLEPNSILFVYGPKSSGKTTVMLRVIEELSERGDLVFFYYDLRKYATPTKEEFLEIFFEKGDKKYLLNKLEINLKIFKLGVEENFDFNNLSLNDIFAKINESINAVVKDRKKPVLIIDELQKLKNIYFNGGKSLLNELFNLFVHLTKVRHLCHVVCLTSDTLFIEEIYQNSTLENTSKYYLIDWLKKEDIKKILKEEGFNDKEINYCLNYLSLPYEIVDLIENKKLGLSVEETIRQWINIEKDRLVYLLKSNKERKENLLKVLSKFKEKIKVNIDDFEDEIFEDVKFLIKNEILFYDVVNGIIKPTSIKKWYAIKEVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-06-02" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEX2</accession>
    <name>F6BEX2_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_0148</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95708.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798317.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF95708">
        <property type="protein sequence ID" value="AEF95708"/>
        <property type="gene ID" value="Metig_0148"/>
    </dbReference>
    <dbReference type="GeneID" id="10642983"/>
    <dbReference type="KEGG" id="mig:Metig_0148"/>
    <dbReference type="HOGENOM" id="CLU_1987633_0_0_2"/>
    <dbReference type="OrthoDB" id="137603at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF95708.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="125" mass="14981" checksum="2D200EF5C3B84128" modified="2011-07-27" version="1">MISEDEFYEKLLEKKKNEEIKVEVKDIDIFQNYRQIDSPDIVLILKLKLNIFSRDFDIEIPIPIEVEKVGLNSALEDLEKFIKREHFKLSLPMLVISEKGFKTDKKEYPLKTEFKIKQIPYYLIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEY4</accession>
    <name>F6BEY4_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_0162</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95720.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798329.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF95720">
        <property type="protein sequence ID" value="AEF95720"/>
        <property type="gene ID" value="Metig_0162"/>
    </dbReference>
    <dbReference type="GeneID" id="10642997"/>
    <dbReference type="KEGG" id="mig:Metig_0162"/>
    <dbReference type="HOGENOM" id="CLU_778079_0_0_2"/>
    <dbReference type="OrthoDB" id="132567at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF95720.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="355" mass="41792" checksum="D9E16C7AF6025C83" modified="2011-07-27" version="1">MGRIYGRVEGPGDIRRINCIIRDEMLSIESVEQLTELKKRSDYLCTLTYSPFWKKKFGFLTEDLRKIAMEENVATTRTANYIARYYDWDKIYNPWGEGEELERELKGLPEEVIKEVSEGAKLKLSPEILEQIRKMYCQVRKAMVFVKSIDELTKLKEATDFFCTLVRTEDFKERFKDYLDKIYDMMEKENERTVKLANIVSVVNGWDIEYEVWSGEEIALEEMVEEYLQRLLEEEEKADKYIPSEAKYGKGITKWITYYHPLKKREYSKRVYFPGDAFDIKIEGPGEFVNRFGRKVWGVKITYKTRIGSRTIERNGISIKIPETVVTRTKVISLPKEAENVRLVDEKPESAYPVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEY9</accession>
    <name>F6BEY9_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">MEMO1 family protein Metig_0168</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_0168</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the MEMO1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95725.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798334.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0168"/>
    <dbReference type="EnsemblBacteria" id="AEF95725">
        <property type="protein sequence ID" value="AEF95725"/>
        <property type="gene ID" value="Metig_0168"/>
    </dbReference>
    <dbReference type="GeneID" id="10643003"/>
    <dbReference type="KEGG" id="mig:Metig_0168"/>
    <dbReference type="HOGENOM" id="CLU_038085_2_0_2"/>
    <dbReference type="OMA" id="EQEAQYG"/>
    <dbReference type="OrthoDB" id="82745at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd07361">
        <property type="entry name" value="MEMO_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00055">
        <property type="entry name" value="MEMO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002737">
        <property type="entry name" value="MEMO1_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11060">
        <property type="entry name" value="PTHR11060"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01875">
        <property type="entry name" value="Memo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF95725.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="286" mass="31521" checksum="E6E342461A96D34B" modified="2011-07-27" version="1">MIRHPAVAGAFYPADPNELIETIEYCYLHNLGPKELPSEGGIFEKPIGLVCPHAGYIYSGPIAAHSYNALSKRVDVEEEITAIILGPNHTGLGTGVATMKGIWKTPLGDLEIDDEFADRLWKECDIMDLDETSHLHEHSIEVQLPFLQHLSMLNIAKFKFVPISMLLQDYETAADIGYFIAKIAKELNRRVVIIASTDFTHYEPQEVAAKKDAIAIKNILNMDEEGLYSDVVNYNISMCGCGPVMAMIKAIKLLGGKEAKLLAYATSGDITKDYSSVVGYASIIIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-06-02" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>F6BF15</accession>
    <name>F6BF15_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_0194</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95751.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798360.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF95751">
        <property type="protein sequence ID" value="AEF95751"/>
        <property type="gene ID" value="Metig_0194"/>
    </dbReference>
    <dbReference type="GeneID" id="10643029"/>
    <dbReference type="KEGG" id="mig:Metig_0194"/>
    <dbReference type="HOGENOM" id="CLU_1881085_0_0_2"/>
    <dbReference type="OMA" id="HNKLELM"/>
    <dbReference type="OrthoDB" id="119878at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF95751.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="135" mass="16062" checksum="B962ECEDE39AEFFC" modified="2011-07-27" version="1">MPKKVHDKLKIMEDILILGKKGEELTIYNITKKLNQNIKNLKCVDIEKHYIKEQTISDAINELKKLNCLEIKSIEKTKTGKEKKIYKLTENSEKIFEIYKKDYSKIHKILKIMRSDEISDDELDIIIFILKRLLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>F6BF46</accession>
    <name>F6BF46_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Acetyl-CoA decarbonylase/synthase complex subunit alpha</fullName>
            <shortName evidence="6">ACDS complex subunit alpha</shortName>
            <ecNumber evidence="6">1.2.7.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">ACDS complex carbon monoxide dehydrogenase subunit alpha</fullName>
            <shortName evidence="6">ACDS CODH subunit alpha</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">cdhA</name>
        <name evidence="8" type="ordered locus">Metig_1381</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Part of the ACDS complex that catalyzes the reversible cleavage of acetyl-CoA, allowing autotrophic growth from CO(2). The alpha-epsilon subcomponent functions as a carbon monoxide dehydrogenase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>CO + H2O + 2 oxidized [2Fe-2S]-[ferredoxin] = CO2 + 2 H(+) + 2 reduced [2Fe-2S]-[ferredoxin]</text>
            <dbReference type="Rhea" id="RHEA:21040"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:17245"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="EC" id="1.2.7.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="6">Binds 7 [4Fe-4S] clusters per heterotetramer.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>[Ni-4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:47739"/>
        </cofactor>
        <text evidence="6">Binds 2 [Ni-4Fe-4S] clusters per heterotetramer.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Heterotetramer of two alpha and two epsilon subunits. The ACDS complex is made up of alpha, epsilon, beta, gamma and delta subunits with a probable stoichiometry of (alpha(2)epsilon(2))(4)-beta(8)-(gamma(1)delta(1))(8).</text>
    </comment>
    <comment type="domain">
        <text evidence="6">Cluster B is an all-cysteinyl-liganded 4Fe-4S cluster; cluster C is a mixed Ni-Fe-S cluster which is the active site of CO oxidation. Cluster D is also an all-cysteinyl-liganded 4Fe-4S cluster that bridges the two subunits of the CODH dimer. Contains two additional 4Fe-4S clusters, dubbed E and F, that probably transport electrons from ferredoxin to the B cluster.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the Ni-containing carbon monoxide dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.2.7.4" evidence="6"/>
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        <property type="protein sequence ID" value="AEF96916.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1381"/>
    <dbReference type="EnsemblBacteria" id="AEF96916">
        <property type="protein sequence ID" value="AEF96916"/>
        <property type="gene ID" value="Metig_1381"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_1381"/>
    <dbReference type="HOGENOM" id="CLU_361186_0_0_2"/>
    <dbReference type="OMA" id="LCIGHNV"/>
    <dbReference type="OrthoDB" id="1404at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0018492">
        <property type="term" value="F:carbon-monoxide dehydrogenase (acceptor) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043885">
        <property type="term" value="F:carbon-monoxide dehydrogenase (ferredoxin) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
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    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
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    <dbReference type="GO" id="GO:0016151">
        <property type="term" value="F:nickel cation binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006084">
        <property type="term" value="P:acetyl-CoA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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    <dbReference type="CDD" id="cd01916">
        <property type="entry name" value="ACS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2030">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01137">
        <property type="entry name" value="CdhA"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004460">
        <property type="entry name" value="CDHA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004137">
        <property type="entry name" value="HCP/CODH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016099">
        <property type="entry name" value="Prismane-like_a/b-sand"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011254">
        <property type="entry name" value="Prismane-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30109">
        <property type="entry name" value="PTHR30109"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03063">
        <property type="entry name" value="Prismane"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56821">
        <property type="entry name" value="SSF56821"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00314">
        <property type="entry name" value="cdhA"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="4 6" id="KW-0408">Iron</keyword>
    <keyword evidence="5 6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1 6" id="KW-0533">Nickel</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="7">
        <location>
            <begin position="406"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="81"/>
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        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="84"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
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            <label>2</label>
        </ligand>
    </feature>
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        <location>
            <position position="85"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
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        </ligand>
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        <location>
            <position position="87"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
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            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="92"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
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        </ligand>
    </feature>
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        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
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        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="125"/>
        </location>
        <ligand>
            <name>CO</name>
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        </ligand>
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        <location>
            <position position="259"/>
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        <ligand>
            <name>[Ni-4Fe-4S] cluster</name>
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        </ligand>
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        <location>
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        <ligand>
            <name>[Ni-4Fe-4S] cluster</name>
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            <name>[Ni-4Fe-4S] cluster</name>
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            <name>[4Fe-4S] cluster</name>
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        <ligand>
            <name>[4Fe-4S] cluster</name>
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        <location>
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        <ligand>
            <name>[4Fe-4S] cluster</name>
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        </ligand>
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        <location>
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        <ligand>
            <name>[4Fe-4S] cluster</name>
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        <location>
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        <ligand>
            <name>[Ni-4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:47739"/>
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        <location>
            <position position="551"/>
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        <ligand>
            <name>[Ni-4Fe-4S] cluster</name>
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        <location>
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        <ligand>
            <name>[Ni-4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:47739"/>
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    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEF96916.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="782" mass="87108" checksum="84F04FFBB0B98A8D" modified="2011-07-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-06-02" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>F6BF63</accession>
    <name>F6BF63_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_1398</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96933.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96933">
        <property type="protein sequence ID" value="AEF96933"/>
        <property type="gene ID" value="Metig_1398"/>
    </dbReference>
    <dbReference type="GeneID" id="10644271"/>
    <dbReference type="KEGG" id="mig:Metig_1398"/>
    <dbReference type="HOGENOM" id="CLU_1544210_0_0_2"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF96933.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="173" mass="20494" checksum="BE811814B8D2750A" modified="2011-07-27" version="1">MANESILEKIESEKRIFVINNDDEVKRYIIKKFEEVKDLDLSDEVLDSSIVEDEKEMIVGVEVNDKIIVDTLTIALARVYQKIDGKLKEKFNYFLNNFEIELYKKSGFKNAPFFKVYVDGKMVISDRRIKIRFSQMGWVKKGNNGRERKEIGSKRFLQEIHVLEDRIMKYLKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>F6BA66</accession>
    <name>F6BA66_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 12">Probable cobalamin biosynthesis protein CobD</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">cobD</name>
        <name evidence="13" type="ordered locus">Metig_0199</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 12">Converts cobyric acid to cobinamide by the addition of aminopropanol on the F carboxylic group.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4 12">Cofactor biosynthesis; adenosylcobalamin biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3 12">Cell membrane</location>
            <topology evidence="3 12">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 12">Belongs to the CobD/CbiB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95756.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798365.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0199"/>
    <dbReference type="EnsemblBacteria" id="AEF95756">
        <property type="protein sequence ID" value="AEF95756"/>
        <property type="gene ID" value="Metig_0199"/>
    </dbReference>
    <dbReference type="GeneID" id="10643034"/>
    <dbReference type="KEGG" id="mig:Metig_0199"/>
    <dbReference type="HOGENOM" id="CLU_054212_0_0_2"/>
    <dbReference type="OMA" id="NSGYTMA"/>
    <dbReference type="OrthoDB" id="101304at2157"/>
    <dbReference type="UniPathway" id="UPA00148"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015420">
        <property type="term" value="F:ABC-type vitamin B12 transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048472">
        <property type="term" value="F:threonine-phosphate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00024">
        <property type="entry name" value="CobD_CbiB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004485">
        <property type="entry name" value="Cobalamin_biosynth_CobD/CbiB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34308">
        <property type="entry name" value="PTHR34308"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03186">
        <property type="entry name" value="CobD_Cbib"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00380">
        <property type="entry name" value="cobD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 12" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 12" id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword evidence="11 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="53"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="85"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="159"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="289"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00006263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00016185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AEF95756.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="309" mass="34581" checksum="D39970ADD31F2BF9" modified="2011-07-27" version="1">MLNPLILFLVVVIDRIFGEPPEKIHPTVLIGKLIAFLEKTFPSSNSKNKFRDFVFGSLVVFTVIFVVFVISILIEKIINSIENEILKDFVYAVILSTTIGYRSLLEFSKKPIDYLKDGDLENARKYVQCIVSRDASKLDEEHVLSAAIESASENITDSIIAPLFYAVIFGLPGAFVYRAINTMDAMMGYKNSKYSYYGKVAARLDDLANLIPSRLAGILLILTAPLYNGDIKKAIYGFLKEGNKTPSPNSGYTMATLANALNITLEKIDHYKLGNGKITIKKSYKALKAIDIVVFVFLLFYLLLFIILM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>F6BA81</accession>
    <name>F6BA81_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">CRISPR-associated endonuclease Cas1</fullName>
            <ecNumber evidence="9">3.1.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">cas1</name>
        <name evidence="10" type="ordered locus">Metig_0214</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Acts as a dsDNA endonuclease. Involved in the integration of spacer DNA into the CRISPR cassette.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="9">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="9">Homodimer, forms a heterotetramer with a Cas2 homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the CRISPR-associated endonuclease Cas1 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="9"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95771.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798380.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0214"/>
    <dbReference type="EnsemblBacteria" id="AEF95771">
        <property type="protein sequence ID" value="AEF95771"/>
        <property type="gene ID" value="Metig_0214"/>
    </dbReference>
    <dbReference type="GeneID" id="10643049"/>
    <dbReference type="KEGG" id="mig:Metig_0214"/>
    <dbReference type="HOGENOM" id="CLU_052779_2_0_2"/>
    <dbReference type="OMA" id="AYKLIKH"/>
    <dbReference type="OrthoDB" id="70037at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004520">
        <property type="term" value="F:endodeoxyribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043571">
        <property type="term" value="P:maintenance of CRISPR repeat elements"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd09722">
        <property type="entry name" value="Cas1_I-B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.100.10.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01470">
        <property type="entry name" value="Cas1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002729">
        <property type="entry name" value="CRISPR-assoc_Cas1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042206">
        <property type="entry name" value="CRISPR-assoc_Cas1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019858">
        <property type="entry name" value="CRISPR-assoc_Cas1_HMARI/TNEAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042211">
        <property type="entry name" value="CRISPR-assoc_Cas1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43219">
        <property type="entry name" value="PTHR43219"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01867">
        <property type="entry name" value="Cas_Cas1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00287">
        <property type="entry name" value="cas1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03641">
        <property type="entry name" value="cas1_HMARI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0051">Antiviral defense</keyword>
    <keyword evidence="7 9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 9" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="4 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8 9" id="KW-0464">Manganese</keyword>
    <keyword evidence="2 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1 9" id="KW-0540">Nuclease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="9">
        <location>
            <position position="149"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="214"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="229"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AEF95771.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="322" mass="37879" checksum="10325BE4E3668A67" modified="2011-07-27" version="1">MRKKALTLFSDGYLFRKENTLYFENVNGKKPLAIEGIYDIYIYGKVSISSQALHFLAQKGIALHFFNHYGYYDGSFYPRESLHSGDLVVKQAEHYLNPEKRLNLAKLFVIGGAKNIEKNLSKFGVKVKFDDFLEELNDAEKITEVMNIEGRMREEYYKFWDETLPDEFKIIKRTRRPPQNEMNALISFLNSRLYPTIISELYNTQLTPTISYLHEPSERRFSLALDLSEIFKPIIADRIANRLVKQRIIKKEHFRDDLNGVLLNDDGKKIVLRAFNEEMEKSVKHPTLKKNVTKKRLIRLEAYKLIKHLTGQKEYEPLIAWF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>F6BA88</accession>
    <name>F6BA88_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Na+/H+ antiporter MnhB subunit-related protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Metig_0221</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95778.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0221"/>
    <dbReference type="EnsemblBacteria" id="AEF95778">
        <property type="protein sequence ID" value="AEF95778"/>
        <property type="gene ID" value="Metig_0221"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0221"/>
    <dbReference type="HOGENOM" id="CLU_069132_3_0_2"/>
    <dbReference type="OMA" id="TAVLFDW"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007182">
        <property type="entry name" value="MnhB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04039">
        <property type="entry name" value="MnhB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="65"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="117"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="144"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="182"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="221"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="domain" description="MnhB" evidence="8">
        <location>
            <begin position="113"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AEF95778.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="259" mass="28759" checksum="E5101AE7FB6226D4" modified="2011-07-27" version="1">MMMSLKREMAVSLSFIFFGASIIYSLYHMEVVSGVNDIYTHTYIIPNLVTAVLFDWRAFDTLGEALILVTSVLVTGMVFGKGLYNYKFLKDVYHAPESDDYLTLSKWEEFTPIIKTLALPMSIFLMALGIIIILGGHITPGGGFQGGALIAAAYILSIVSFGSKSPLWFKHKFLEKLETFGALMFMIFGLIGMVVSGYYLFNFNELFGFAVFPSPIELKNAGIIPYLNISVGLKVLAGLSTLTFLLTCEKVIIEKIMDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAA2</accession>
    <name>F6BAA2_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Putative asparagine synthetase [glutamine-hydrolyzing]</fullName>
            <ecNumber evidence="4">6.3.5.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Metig_0235</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>ATP + H2O + L-aspartate + L-glutamine = AMP + diphosphate + H(+) + L-asparagine + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:12228"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58048"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.5.4"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="6.3.5.4" evidence="4"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95792.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798401.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0235"/>
    <dbReference type="EnsemblBacteria" id="AEF95792">
        <property type="protein sequence ID" value="AEF95792"/>
        <property type="gene ID" value="Metig_0235"/>
    </dbReference>
    <dbReference type="GeneID" id="10643070"/>
    <dbReference type="KEGG" id="mig:Metig_0235"/>
    <dbReference type="HOGENOM" id="CLU_014658_3_1_2"/>
    <dbReference type="OMA" id="IEHSHQP"/>
    <dbReference type="OrthoDB" id="12911at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004066">
        <property type="term" value="F:asparagine synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006529">
        <property type="term" value="P:asparagine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006541">
        <property type="term" value="P:glutamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01991">
        <property type="entry name" value="Asn_Synthase_B_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00712">
        <property type="entry name" value="AsnB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006426">
        <property type="entry name" value="Asn_synth_AEB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001962">
        <property type="entry name" value="Asn_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033738">
        <property type="entry name" value="AsnB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017932">
        <property type="entry name" value="GATase_2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00733">
        <property type="entry name" value="Asn_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13537">
        <property type="entry name" value="GATase_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001589">
        <property type="entry name" value="Asn_synthetase_glu-h"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="SSF56235"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01536">
        <property type="entry name" value="asn_synth_AEB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51278">
        <property type="entry name" value="GATASE_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="5" id="KW-0061">Asparagine biosynthesis</keyword>
    <keyword evidence="2 4 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 5" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="9" id="KW-0436">Ligase</keyword>
    <keyword evidence="1 4 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutamine amidotransferase type-2" evidence="8">
        <location>
            <begin position="2"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="active site" description="For GATase activity" evidence="5">
        <location>
            <position position="2"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="109"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="301"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="373"/>
            <end position="374"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="site" description="Important for beta-aspartyl-AMP intermediate formation" evidence="7">
        <location>
            <position position="375"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR001589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR001589-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR001589-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR001589-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AEF95792.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="624" mass="73855" checksum="B3DAB9284420AD83" modified="2011-07-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-06-02" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAB7</accession>
    <name>F6BAB7_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_0251</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95807.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798416.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF95807">
        <property type="protein sequence ID" value="AEF95807"/>
        <property type="gene ID" value="Metig_0251"/>
    </dbReference>
    <dbReference type="GeneID" id="10643086"/>
    <dbReference type="KEGG" id="mig:Metig_0251"/>
    <dbReference type="HOGENOM" id="CLU_1891439_0_0_2"/>
    <dbReference type="OrthoDB" id="143678at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="107"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="7"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF95807.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="134" mass="15436" checksum="C9DF44A90E7D5DBD" modified="2011-07-27" version="1">MSSATSVDELLVRIQKLEKEVEKLKEKNDAESKEIAISIAREEVKHQSSIIKNEVRDELRKELATKEDILLVEGKLSGEIKTMEERLEIKMALLEKKIGLTNQKMDYWVKILIILIIISPFIPEVLKNIGLLLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAB8</accession>
    <name>F6BAB8_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">PfkB domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0252</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95808.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798417.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0252"/>
    <dbReference type="EnsemblBacteria" id="AEF95808">
        <property type="protein sequence ID" value="AEF95808"/>
        <property type="gene ID" value="Metig_0252"/>
    </dbReference>
    <dbReference type="GeneID" id="10643087"/>
    <dbReference type="KEGG" id="mig:Metig_0252"/>
    <dbReference type="HOGENOM" id="CLU_021150_0_1_2"/>
    <dbReference type="OMA" id="CEFANAA"/>
    <dbReference type="OrthoDB" id="36919at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016773">
        <property type="term" value="F:phosphotransferase activity, alcohol group as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01172">
        <property type="entry name" value="RfaE_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011611">
        <property type="entry name" value="PfkB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011913">
        <property type="entry name" value="RfaE_dom_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00294">
        <property type="entry name" value="PfkB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="SSF53613"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PfkB" evidence="1">
        <location>
            <begin position="2"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF95808.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="312" mass="35035" checksum="549EE548A07278CC" modified="2011-07-27" version="1">MIVVLGEVMLDKYTYGKVERINPEAPVPVLNAVEEKYTLGGAGNVANNIASLKHDVFLISITNRDEFGKCIEKLCNENGIKYYFVSDGRPTIVKHRFVAIGYNQQLLRVDYEKIYPINEEISNKILDKIKRLNSKSNILIISDYAKGLITKELMEKIKKEFKGKILVDPKPKNIEFYKDVYLIKPNLKETSQIIGREIENKDDELEKIGLELVEEFNSNFVITRSEKGSTLVTLNGEVYHISTKVKEVHDVSGAGDTFIAVLAYALDKGYDLVDAVKLANKASSIVVGKVGTATVSLDELFGKFSENNKMET</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAE8</accession>
    <name>F6BAE8_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Radical SAM domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Metig_1426</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="5">Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96961.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96961">
        <property type="protein sequence ID" value="AEF96961"/>
        <property type="gene ID" value="Metig_1426"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_1426"/>
    <dbReference type="HOGENOM" id="CLU_062674_0_1_2"/>
    <dbReference type="OMA" id="GGEPTPN"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040085">
        <property type="entry name" value="MJ0674-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016431">
        <property type="entry name" value="Pyrv-formate_lyase-activ_prd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004869">
        <property type="entry name" value="PflX_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01099">
        <property type="entry name" value="Uncharacterised_Radical_SAM_Su"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 5" id="KW-0408">Iron</keyword>
    <keyword evidence="4 5" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="6">
        <location>
            <begin position="117"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="135"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="139"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="142"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR004869-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEF96961.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="343" mass="40461" checksum="D8C1499A05DA9C0A" modified="2011-07-27" version="1">MYSIGEKMLLERYLKVSENKLPAKFLISKSIKVDNFAKKDIDELWEMHKNAMDEFKETTNIKDIKITHPNLLDLKIEIAKKIFENCHFCEHKCYVNRKKELGFCKIGESYYSSEFLHYGEEDVLIPSHTIFFCGCNFKCVFCQNWEISQIYFENLPVENYCKKVNRKLMAKIIEEKRAISKNVNFVGGEPTPHLLTILEILRHVNVNIPVVWNSNMYMSEEAMHLLDGVVDLWLSDFKFGNDRCAERLSKIKNYCKVVKRNHLMIKGEDIIIRHLVMPNHLECCTERIFKWIGKHLDAKVNVMFQYRPEYKAILYEDIDRALNHEEMAKALLLAKKYGLDTDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-06-02" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAG4</accession>
    <name>F6BAG4_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1442</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96977.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799573.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96977">
        <property type="protein sequence ID" value="AEF96977"/>
        <property type="gene ID" value="Metig_1442"/>
    </dbReference>
    <dbReference type="GeneID" id="10644315"/>
    <dbReference type="KEGG" id="mig:Metig_1442"/>
    <dbReference type="HOGENOM" id="CLU_2893255_0_0_2"/>
    <dbReference type="OMA" id="TKITAIY"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="35"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96977.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="62" mass="7251" checksum="15B0DAF56F2120F0" modified="2011-07-27" version="1">MDKILKLFIKFLVLFIGFIILDFVVEYYLFDGLAVYLYVSAAMVFVVLMILVIIYTKITAIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAG9</accession>
    <name>F6BAG9_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcriptional regulator, XRE family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1447</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96982.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799578.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1447"/>
    <dbReference type="EnsemblBacteria" id="AEF96982">
        <property type="protein sequence ID" value="AEF96982"/>
        <property type="gene ID" value="Metig_1447"/>
    </dbReference>
    <dbReference type="GeneID" id="10644320"/>
    <dbReference type="KEGG" id="mig:Metig_1447"/>
    <dbReference type="HOGENOM" id="CLU_077869_0_0_2"/>
    <dbReference type="OMA" id="KMAGEIT"/>
    <dbReference type="OrthoDB" id="55424at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028979">
        <property type="entry name" value="Ser_kin/Pase_Hpr-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017271">
        <property type="entry name" value="Tscrpt_reg_HTH_MJ1545_prd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01381">
        <property type="entry name" value="HTH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037724">
        <property type="entry name" value="TF_HTH_MJ1545_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00530">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75138">
        <property type="entry name" value="SSF75138"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH cro/C1-type" evidence="1">
        <location>
            <begin position="22"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96982.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="229" mass="25651" checksum="8E8947304BE7B60B" modified="2011-07-27" version="1">MEKIAIHITGDIVLSENPGKAMKKWRELFNIPQVEVARYLGVSPSVVSDYEVGRRKNPGVSIIKKYVHALIEIDKERGGHTIKALKRILDTSPSIKAILSIKEYENPISIEEFANIIDAKIVYGENLGNVIYGHTVVDSIKAILEMDGQDFLNLYGWTTERALVFTNVSSGRSPMVAIRVSNMKPRVVVFQGVQEIDTLAIKLAEVENIPLLITKLDIKELLKRLSDIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAJ5</accession>
    <name>F6BAJ5_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="4">DNA-cytosine methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metig_1474</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97008.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799604.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1474"/>
    <dbReference type="REBASE" id="36261">
        <property type="entry name" value="M.Mig5ORF1474P"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF97008">
        <property type="protein sequence ID" value="AEF97008"/>
        <property type="gene ID" value="Metig_1474"/>
    </dbReference>
    <dbReference type="GeneID" id="10644347"/>
    <dbReference type="KEGG" id="mig:Metig_1474"/>
    <dbReference type="HOGENOM" id="CLU_006958_2_2_2"/>
    <dbReference type="OMA" id="HPFTPRC"/>
    <dbReference type="OrthoDB" id="58937at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001525">
        <property type="entry name" value="C5_MeTfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00145">
        <property type="entry name" value="DNA_methylase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00105">
        <property type="entry name" value="C5METTRFRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51679">
        <property type="entry name" value="SAM_MT_C5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3 4" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2 3 4" id="KW-0808">Transferase</keyword>
    <feature type="active site" evidence="3">
        <location>
            <position position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEF97008.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="312" mass="36159" checksum="F2888D4D45662433" modified="2011-07-27" version="1">MEEKPNVIDLFCGCGGFSIGFKKHFNILGGIENFKPSAKTYMYNVGAKVWVDDIKRIPPMAFDEFINKEKVDVIIGSPPCEPFTKANIKIKDNPVDRLYKDKIGQLVLYYINYVDYFTKKNDDLIFIMENVPHIKEIKDELKKLFGDIGHKVYFNILRAEDYGNPSKRARMFISNIKLKPKKREKRVVIDALSNIDENAPNHEIQKLSPKKLALISKLKWGESLYKFKGKNKIMENWFRLHPYKLAPTVKGKGRFIHPFEDRLLTVREQARLMGFDDNFVFFGGRNTQYNMIGESVPPTLSRAIAEVIKDNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAN4</accession>
    <name>F6BAN4_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">DNA polymerase beta domain protein region</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_0292</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95848.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798457.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0292"/>
    <dbReference type="EnsemblBacteria" id="AEF95848">
        <property type="protein sequence ID" value="AEF95848"/>
        <property type="gene ID" value="Metig_0292"/>
    </dbReference>
    <dbReference type="GeneID" id="10643127"/>
    <dbReference type="KEGG" id="mig:Metig_0292"/>
    <dbReference type="HOGENOM" id="CLU_072733_0_0_2"/>
    <dbReference type="OMA" id="FTHTYAG"/>
    <dbReference type="OrthoDB" id="40897at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF95848.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="340" mass="40248" checksum="17173C87FAF17737" modified="2011-07-27" version="1">MRVRLRDFVETKEGLFFAVNTYYHPEDRVLCFLRYVNLDLVKDENLNLNSNNIRILNGKKYIKMAETEKAYKFLENYFPEYLFYDEVNDVLMHSVPNERIKRILRPNERLKELLNLDNPNELEEKCIKLADILHDYGIPYKHMGISGSMVLKLSNSNSDIDFVIYGMENHKNAREALKNAFEDKKLSPLSNEFWKKAYEKRIKDNTLTFEEFVWHEKRKFNRGVIDGVMFDLLATREWDEITEKYGEKRYRSLGFVEVEGVVVDATYAFDNPAVYRIECDDDEIKEVVSFTHTYAGQCFEEEVVVVRGKLEEVIYNGESYKRVVVGTSREAFNEYIKLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAP8</accession>
    <name>F6BAP8_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Metig_0306</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95862.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798471.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF95862">
        <property type="protein sequence ID" value="AEF95862"/>
        <property type="gene ID" value="Metig_0306"/>
    </dbReference>
    <dbReference type="GeneID" id="10643141"/>
    <dbReference type="KEGG" id="mig:Metig_0306"/>
    <dbReference type="HOGENOM" id="CLU_134207_1_0_2"/>
    <dbReference type="OMA" id="NGEYIHN"/>
    <dbReference type="OrthoDB" id="117291at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026485">
        <property type="entry name" value="Archaeo_ArtE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026392">
        <property type="entry name" value="Exo/Archaeosortase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019127">
        <property type="entry name" value="Exosortase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09721">
        <property type="entry name" value="Exosortase_EpsH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR04124">
        <property type="entry name" value="archaeo_artE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR04178">
        <property type="entry name" value="exo_archaeo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="7"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="63"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="90"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="129"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AEF95862.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="168" mass="19400" checksum="135B9A65D5D46581" modified="2011-07-27" version="1">MDKWIRTILKFFILTPIVYVIMKPLEDFLIKYVAFHSYLILKMFEPCYLEDSVIHLSNLSIEIIGVCTGVTLMAIYISLVVVTAKNFKEFFIGSLVGLFLIYLGNVLRICLIAFIGTHESYNIQTIHDIISYIDVPITVFASILWLKIQNLMDKSNKISKKIKKIKED</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAR2</accession>
    <name>F6BAR2_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Formylmethanofuran dehydrogenase subunit A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0320</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95876.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798485.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0320"/>
    <dbReference type="EnsemblBacteria" id="AEF95876">
        <property type="protein sequence ID" value="AEF95876"/>
        <property type="gene ID" value="Metig_0320"/>
    </dbReference>
    <dbReference type="GeneID" id="10643156"/>
    <dbReference type="KEGG" id="mig:Metig_0320"/>
    <dbReference type="HOGENOM" id="CLU_035587_0_0_2"/>
    <dbReference type="OMA" id="WAMGLEL"/>
    <dbReference type="OrthoDB" id="8152at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016810">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01304">
        <property type="entry name" value="FMDH_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013108">
        <property type="entry name" value="Amidohydro_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012027">
        <property type="entry name" value="Formylmethanofuran_DH_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011059">
        <property type="entry name" value="Metal-dep_hydrolase_composite"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07969">
        <property type="entry name" value="Amidohydro_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006453">
        <property type="entry name" value="FwdA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51338">
        <property type="entry name" value="SSF51338"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="SSF51556"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03121">
        <property type="entry name" value="one_C_dehyd_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidohydro_3" evidence="1">
        <location>
            <begin position="43"/>
            <end position="517"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF95876.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="581" mass="65113" checksum="468B2EF5CAB4B062" modified="2011-07-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAS8</accession>
    <name>F6BAS8_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Selenocysteine-specific elongation factor</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">SelB translation factor</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Metig_0336</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Translation factor necessary for the incorporation of selenocysteine into proteins. It probably replaces EF-Tu for the insertion of selenocysteine directed by the UGA codon. SelB binds GTP and GDP.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95892.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0336"/>
    <dbReference type="EnsemblBacteria" id="AEF95892">
        <property type="protein sequence ID" value="AEF95892"/>
        <property type="gene ID" value="Metig_0336"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0336"/>
    <dbReference type="HOGENOM" id="CLU_019148_1_0_2"/>
    <dbReference type="OMA" id="FGHIDHG"/>
    <dbReference type="OrthoDB" id="76962at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001514">
        <property type="term" value="P:selenocysteine incorporation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038661">
        <property type="entry name" value="L35A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009001">
        <property type="entry name" value="Transl_elong_EF1A/Init_IF2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004535">
        <property type="entry name" value="Transl_elong_SelB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="SSF50447"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50465">
        <property type="entry name" value="SSF50465"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00475">
        <property type="entry name" value="selB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="6">
        <location>
            <begin position="1"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00015953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00025526"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00031615"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEF95892.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="466" mass="51273" checksum="E8A68C3DB6AF40E1" modified="2011-07-27" version="1">MINVNLGIFGHIDHGKTLLAKQLTEIASTSALDKPPESKRRGITIDIGFSSFTLDNYRITLVDAPGHADLIKAVVGAADIIDLALLVVDAREGPKTQTGEHLLILDLFNIPTIVVMNKIDIASEDEIKNTETFMRAILNSTNNLKNAKIVKISAKEGIGIDELKKTLKETLDNMEIKRDVDNYFKMPIDHAFSIKGVGTVVTGTIHKGKVSVGDEMRTFPINVDVKVKGIQCFKKDVNEAYAGDRVGIALQGVEPKQIFRGCVLTSKDTKLEVVDKIVAKVKISEIFKYNITPKMKVHLNVGMMVVPATVVPFQKMNFEGKEVNVILNEVKGGDECYCAFELEEKVVAEIGDRVLITRLDLPPTTLRICGHGEIVEFKGVKELNIKKIVTREGKVVAKKDKLFVEGLASSKTSAEKLIGEVVYVPSKNIKGVIKGTFGTKGHLVVEFDGDVEKGDTVILRRLRNWG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAT4</accession>
    <name>F6BAT4_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Phosphoribosylaminoimidazole-succinocarboxamide synthase</fullName>
            <ecNumber evidence="8">6.3.2.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">SAICAR synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">purC</name>
        <name evidence="10" type="ordered locus">Metig_0342</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate + ATP + L-aspartate = (2S)-2-[5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamido]succinate + ADP + 2 H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:22628"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58443"/>
            <dbReference type="ChEBI" id="CHEBI:77657"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.2.6"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 8">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the SAICAR synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.2.6" evidence="8"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95898.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798507.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0342"/>
    <dbReference type="EnsemblBacteria" id="AEF95898">
        <property type="protein sequence ID" value="AEF95898"/>
        <property type="gene ID" value="Metig_0342"/>
    </dbReference>
    <dbReference type="GeneID" id="10643179"/>
    <dbReference type="KEGG" id="mig:Metig_0342"/>
    <dbReference type="HOGENOM" id="CLU_061495_2_0_2"/>
    <dbReference type="OMA" id="EFCYKND"/>
    <dbReference type="OrthoDB" id="47738at2157"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00131"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004639">
        <property type="term" value="F:phosphoribosylaminoimidazolesuccinocarboxamide synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01415">
        <property type="entry name" value="SAICAR_synt_PurC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00137">
        <property type="entry name" value="SAICAR_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028923">
        <property type="entry name" value="SAICAR_synt/ADE2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033934">
        <property type="entry name" value="SAICAR_synt_PurC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001636">
        <property type="entry name" value="SAICAR_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018236">
        <property type="entry name" value="SAICAR_synthetase_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01259">
        <property type="entry name" value="SAICAR_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00081">
        <property type="entry name" value="purC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01057">
        <property type="entry name" value="SAICAR_SYNTHETASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 8 10" id="KW-0436">Ligase</keyword>
    <keyword evidence="5 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 8" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SAICAR_synt" evidence="9">
        <location>
            <begin position="12"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AEF95898.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="240" mass="27335" checksum="857BFB0F99BBD088" modified="2011-07-27" version="1">MDVNEIIKNPPIHKGKAKSVYEIDDEKVLIEFRDDITAGNGAKHDVKENKGFLNALISTKLFKVLEENGVPTHFIEYIEPRYMVAKKVEIIPIEVIVRNIAAGSLCRRYPFKEGQELNPPIVQFDYKNDEYGDPMLNDDIAIALGLATKEELEEIKNIALKVNEVLKKFFDEKGIILVDFKIEVGRTKDGKIVVADEISPDTMRLWDKETKDVLDKDVFRKDLGDVIAKYRIVAEKIGCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>F6BB14</accession>
    <name>F6BB14_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ATPase-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1567</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97101.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799695.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF97101">
        <property type="protein sequence ID" value="AEF97101"/>
        <property type="gene ID" value="Metig_1567"/>
    </dbReference>
    <dbReference type="GeneID" id="10644440"/>
    <dbReference type="KEGG" id="mig:Metig_1567"/>
    <dbReference type="HOGENOM" id="CLU_063816_1_0_2"/>
    <dbReference type="OMA" id="FMTTHSQ"/>
    <dbReference type="OrthoDB" id="38516at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13304">
        <property type="entry name" value="AAA_21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATPase_AAA_core" evidence="1">
        <location>
            <begin position="24"/>
            <end position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF97101.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="369" mass="42391" checksum="2A37651B4729EBD8" modified="2011-07-27" version="1">MFKKVIINSFRSINNLEIDDLKKVNLFVGKNNCGKTSVLEGIYLLSNPMSILNLVNIRNFRKMYIDSEVFNNALLTIFNNMDTDIPVKIEGFINSYKKKELTINYIDESKIRYDENIGGFPIGGLHLNYITFEGSNITDEKCYEVSIWERGERLKNKIVIKENNSIESVVPFPVGVRISQIASLTKEQKSVYLSPKMDMNDIAGWLNKLQISKEIGEIIEILQEIEPSIKDLRIGADGIIYCDVGVKKLIPINVMGDGILKLLIILSAIYNAKGGFVFIDEIENGLHYSSLEILWKGIFEASKKFNVQIFATTHSWECIEAFSNVHENDAEEGVDIRLYRIERYENELKAIDYDRETIKEAIKMKWEMR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>F6BB21</accession>
    <name>F6BB21_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Radical SAM domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Metig_1574</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97108.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799702.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1574"/>
    <dbReference type="EnsemblBacteria" id="AEF97108">
        <property type="protein sequence ID" value="AEF97108"/>
        <property type="gene ID" value="Metig_1574"/>
    </dbReference>
    <dbReference type="GeneID" id="10644447"/>
    <dbReference type="KEGG" id="mig:Metig_1574"/>
    <dbReference type="HOGENOM" id="CLU_048071_0_0_2"/>
    <dbReference type="OMA" id="CIFCSVD"/>
    <dbReference type="OrthoDB" id="68339at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaB/NifB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040088">
        <property type="entry name" value="MJ0103-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002792">
        <property type="entry name" value="TRAM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11228:SF35">
        <property type="entry name" value="PTHR11228:SF35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01110">
        <property type="entry name" value="Uncharacterised_Radical_SAM_Su"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50926">
        <property type="entry name" value="TRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="6">
        <location>
            <begin position="109"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="domain" description="TRAM" evidence="5">
        <location>
            <begin position="375"/>
            <end position="438"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEF97108.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="438" mass="50005" checksum="A7F8D13C4E96F103" modified="2011-07-27" version="1">MELNEKNNILDLSSFRMITGVKSKDNKLILEINKNYECEVEIPYNVDIEDDKIIINEHPLKVENIKRGILNLISLSISQNLVNKITNRRTYYIAPPIPLIGHTAFGLIDRGTNIIQVRGLSGCNINCPFCSVDEGKHSKTRKNDYYVDVDYLVEEYKKIVEFKGNKFIEAHLDGQGEPTLYYPLADLVQNLSEINKEGKGIVSMQSNGVGLTYKMIDELEEAGLHRINLSINALDEKMAKILAGRKDYNIEKILDIAEYIKNSKIHLLIAPILLPNVNDEEVKKVIDYAIELDQRIPQNVINPITNKKDPILGVQLCLIYQFGRRVKKMKIWEFKKFYKLLKDYEAQYGAKGIDVKLKLHPSDFGTHRRKKLPCPFKVGEVVNVDVVMDGRIKGEVIGVAKNRIVQIINCKNEHKLIGNNIRVKILRTKDNIIVSTPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>F6BB23</accession>
    <name>F6BB23_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcriptional regulator, ArsR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1576</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97110.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799704.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1576"/>
    <dbReference type="EnsemblBacteria" id="AEF97110">
        <property type="protein sequence ID" value="AEF97110"/>
        <property type="gene ID" value="Metig_1576"/>
    </dbReference>
    <dbReference type="GeneID" id="10644449"/>
    <dbReference type="KEGG" id="mig:Metig_1576"/>
    <dbReference type="HOGENOM" id="CLU_097806_3_5_2"/>
    <dbReference type="OMA" id="MCVCELE"/>
    <dbReference type="OrthoDB" id="111287at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001845">
        <property type="entry name" value="HTH_ArsR_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01022">
        <property type="entry name" value="HTH_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00778">
        <property type="entry name" value="HTHARSR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00418">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50987">
        <property type="entry name" value="HTH_ARSR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH arsR-type" evidence="1">
        <location>
            <begin position="1"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF97110.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="92" mass="10655" checksum="96814C857225A27E" modified="2011-07-27" version="1">MDNGKYEAMAEVFKAFADPTRLMILKLLTENESMCVCKIIDELKKPQPTISHHLNILKKAGIIKAKKEGTWNYYYIVNPKVKELIQMIDEIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>F6BB54</accession>
    <name>F6BB54_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="7">ABC-type transporter, integral membrane subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Metig_0383</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95939.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798548.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0383"/>
    <dbReference type="EnsemblBacteria" id="AEF95939">
        <property type="protein sequence ID" value="AEF95939"/>
        <property type="gene ID" value="Metig_0383"/>
    </dbReference>
    <dbReference type="GeneID" id="10643220"/>
    <dbReference type="KEGG" id="mig:Metig_0383"/>
    <dbReference type="HOGENOM" id="CLU_039929_3_0_2"/>
    <dbReference type="OMA" id="VAGIMWG"/>
    <dbReference type="OrthoDB" id="82041at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001851">
        <property type="entry name" value="ABC_transp_permease"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02653">
        <property type="entry name" value="BPD_transp_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="41"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="65"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="94"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="141"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="187"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="220"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="269"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEF95939.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="294" mass="31305" checksum="FF6917A595E0FF9C" modified="2011-07-27" version="1">MDIAQILVDTVVLGSVYVLVAVGLTMTYGLLKFANFAHAEFITFGAYVGLVAVTMLNLGLVGGGILAFILTGLLGILGYLLVFKPLQKRGANSVELMIASIGVGLIIRHILQQIFGAPMKTYGIVRHIYEIGSVRITNLEILEIICAFAFIIIFHIILHKTKLGKAMRAVSNNPSLAMASGINVDKIILWVWFIGAGAAGLGGMLFAANTRVIPTFGWEILLPVFAVVVLGGIGSVYGTFLAAFIIALAENIGVVLLSELGLSTGYRPAVAFIILILVLLIRPQGLISLKTRVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBA2</accession>
    <name>F6BBA2_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Peroxiredoxin</fullName>
            <ecNumber evidence="5">1.11.1.24</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Thioredoxin peroxidase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">Thioredoxin-dependent peroxiredoxin</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Metig_0431</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>[thioredoxin]-dithiol + a hydroperoxide = [thioredoxin]-disulfide + an alcohol + H2O</text>
            <dbReference type="Rhea" id="RHEA:62620"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:30879"/>
            <dbReference type="ChEBI" id="CHEBI:35924"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="EC" id="1.11.1.24"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodecamer. Pentamer of dimers that assemble into a ring structure.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="miscellaneous">
        <text evidence="5">The active site is a conserved redox-active cysteine residue, the peroxidatic cysteine (C(P)), which makes the nucleophilic attack on the peroxide substrate. The peroxide oxidizes the C(P)-SH to cysteine sulfenic acid (C(P)-SOH), which then reacts with another cysteine residue, the resolving cysteine (C(R)), to form a disulfide bridge. The disulfide is subsequently reduced by an appropriate electron donor to complete the catalytic cycle. Although the primary sequence of this enzyme is similar to those of the 1-Cys Prx6 enzymes, its catalytic properties resemble those of the typical 2-Cys Prxs and C(R) is provided by the other dimeric subunit to form an intersubunit disulfide. The disulfide is subsequently reduced by thioredoxin.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 5">Belongs to the peroxiredoxin family. Prx6 subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.11.1.24" evidence="5"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95987.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0431"/>
    <dbReference type="EnsemblBacteria" id="AEF95987">
        <property type="protein sequence ID" value="AEF95987"/>
        <property type="gene ID" value="Metig_0431"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0431"/>
    <dbReference type="HOGENOM" id="CLU_042529_4_4_2"/>
    <dbReference type="OMA" id="FWYPKDF"/>
    <dbReference type="OrthoDB" id="98508at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008379">
        <property type="term" value="F:thioredoxin peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03016">
        <property type="entry name" value="PRX_1cys"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00401">
        <property type="entry name" value="Peroxiredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000866">
        <property type="entry name" value="AhpC/TSA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024706">
        <property type="entry name" value="Peroxiredoxin_AhpC-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019479">
        <property type="entry name" value="Peroxiredoxin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022915">
        <property type="entry name" value="Peroxiredoxin_TDXH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045020">
        <property type="entry name" value="PRX_1cys"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10417">
        <property type="entry name" value="1-cysPrx_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00578">
        <property type="entry name" value="AhpC-TSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000239">
        <property type="entry name" value="AHPC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0049">Antioxidant</keyword>
    <keyword evidence="1 5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3 5 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5 8" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="5" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="7">
        <location>
            <begin position="15"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="active site" description="Cysteine sulfenic acid (-SOH) intermediate" evidence="5 6">
        <location>
            <position position="57"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="133"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="disulfide bond" description="Interchain (with Cys-223); in linked form" evidence="5">
        <location>
            <position position="57"/>
        </location>
    </feature>
    <feature type="disulfide bond" description="Alternate" evidence="5">
        <location>
            <begin position="217"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="disulfide bond" description="Interchain (with Cys-57); in linked form" evidence="5">
        <location>
            <position position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00025719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR000239-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEF95987.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="228" mass="26220" checksum="D34B08C317AEEE9C" modified="2011-07-27" version="1">MCEETICCIEGGRMPLIGEKFPEVEVKTTHGVIKLPDHYKGKWFVLFSHPADFTPVCTTEFVGFQKRYKEFKELNTELIGLSIDQVFSHLKWIEWIKEKLNVEIEFPVIADDRGILAEQLGMISPYKGNNTVRAVFVVDDKGIIRAIIYYPQEVGRNLDEIVRLVKALQTSDKKGMAMPANWPNNDILGDKVIIPPASTIEEIKQRKEACKKGEIECLDWWLCYKKLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBB3</accession>
    <name>F6BBB3_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_1587</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97120.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799713.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF97120">
        <property type="protein sequence ID" value="AEF97120"/>
        <property type="gene ID" value="Metig_1587"/>
    </dbReference>
    <dbReference type="GeneID" id="10644460"/>
    <dbReference type="KEGG" id="mig:Metig_1587"/>
    <dbReference type="HOGENOM" id="CLU_611953_0_0_2"/>
    <dbReference type="OMA" id="TEWIPFG"/>
    <dbReference type="OrthoDB" id="22718at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="421"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="323"/>
            <end position="350"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF97120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="464" mass="53728" checksum="627D6C429DAA9765" modified="2011-07-27" version="1">MSKTFTHFVKLKNPKVVITEWIPFGKNYMTEFIDRVTLKEYQKKRVKYFTEVVDNISTIEPVFKTSKYYTKVNIIDFEPEMYVKFIAEVNFEKEGIFQEKDVFIYVDYLGRCIYASEIESAGDEEVYISLDNLAFIIPEMIMDTSKMIKSLIASSQRYIIETLYGQYKVYKHVSILTETIFEINENTLAEVRQVIGNVKRIIDIGEGKLIFGEFGMLLMHPNPKKFENFISCYSFIRSVVQSSQDLFFKLNNISTRLERLSNILTGKIDVETITKLRNELSEIDKELAVIEIVSGYLKEISSLMNEEKFLSKFTGEDARLLKMINAKNKLKKLNNRIIEIENAIKSNLELATSLTRLLTTLSDNLQSKISNQLAESVKHQVAIGEAMELLEAGIFGVYALEAIHILLLLSGKEHELMHYKVLGFPIPFWIVLFAVIFGIYIGKKILEYRKRKVLEEYLATHEID</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBB5</accession>
    <name>F6BBB5_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RNA 2',3'-cyclic phosphodiesterase</fullName>
            <shortName evidence="2">RNA 2',3'-CPDase</shortName>
            <ecNumber evidence="2">3.1.4.58</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metig_1589</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Hydrolyzes RNA 2',3'-cyclic phosphodiester to an RNA 2'-phosphomonoester.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>a 3'-end 2',3'-cyclophospho-ribonucleotide-RNA + H2O = a 3'-end 2'-phospho-ribonucleotide-RNA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11828"/>
            <dbReference type="Rhea" id="RHEA-COMP:10464"/>
            <dbReference type="Rhea" id="RHEA-COMP:17353"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:83064"/>
            <dbReference type="ChEBI" id="CHEBI:173113"/>
            <dbReference type="EC" id="3.1.4.58"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the 2H phosphoesterase superfamily. ThpR family.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.58" evidence="2"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97122.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799715.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1589"/>
    <dbReference type="EnsemblBacteria" id="AEF97122">
        <property type="protein sequence ID" value="AEF97122"/>
        <property type="gene ID" value="Metig_1589"/>
    </dbReference>
    <dbReference type="GeneID" id="10644462"/>
    <dbReference type="KEGG" id="mig:Metig_1589"/>
    <dbReference type="HOGENOM" id="CLU_081251_3_4_2"/>
    <dbReference type="OMA" id="HITLRFL"/>
    <dbReference type="OrthoDB" id="102399at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004113">
        <property type="term" value="F:2',3'-cyclic-nucleotide 3'-phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008664">
        <property type="term" value="F:2'-5'-RNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01940">
        <property type="entry name" value="RNA_CPDase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009097">
        <property type="entry name" value="Cyclic_Pdiesterase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014051">
        <property type="entry name" value="Phosphoesterase_HXTX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004175">
        <property type="entry name" value="RNA_CPDase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35561">
        <property type="entry name" value="PTHR35561"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02834">
        <property type="entry name" value="LigT_PEase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55144">
        <property type="entry name" value="SSF55144"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02258">
        <property type="entry name" value="2_5_ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0436">Ligase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="LigT_PEase" evidence="3">
        <location>
            <begin position="7"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="domain" description="LigT_PEase" evidence="3">
        <location>
            <begin position="90"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="HXTX 1" evidence="2">
        <location>
            <begin position="38"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="HXTX 2" evidence="2">
        <location>
            <begin position="116"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="2">
        <location>
            <position position="38"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="2">
        <location>
            <position position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEF97122.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="171" mass="20039" checksum="ACF2A1CCD9480ABC" modified="2011-07-27" version="1">MRCFIAIDLPEEIKDKIEEVKKHFKIKGIKLVEKENLHITIKFLGEIDEEIVEKIKNLDLSISPIKVKIKNIGVFPNENYIRVIWLGATNLVDLFKEVDEKLSEIGFKKEREYVPHVTIGRVKFIENKKALKDKIEKHRKIDIGEFEVKSIKLMKSTLTPNGPIYEVIKEW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-06-02" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBE6</accession>
    <name>F6BBE6_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1620</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97153.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799746.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF97153">
        <property type="protein sequence ID" value="AEF97153"/>
        <property type="gene ID" value="Metig_1620"/>
    </dbReference>
    <dbReference type="GeneID" id="10644493"/>
    <dbReference type="KEGG" id="mig:Metig_1620"/>
    <dbReference type="HOGENOM" id="CLU_188101_0_0_2"/>
    <dbReference type="OMA" id="HKMEDIE"/>
    <dbReference type="OrthoDB" id="140132at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="5"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="30"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="59"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF97153.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="85" mass="9724" checksum="BD454A927EF3BA0A" modified="2011-07-27" version="1">MLYLYLLSIAGLITVLYIISVSIYQLNIMSLSLIFVGIFVGILLLKYKKICHLMEKLEIAFMFLVLIGFAYLGFCLYSVGWLYAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBF9</accession>
    <name>F6BBF9_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 4">Orotate phosphoribosyltransferase</fullName>
            <shortName evidence="4">OPRT</shortName>
            <shortName evidence="4">OPRTase</shortName>
            <ecNumber evidence="2 4">2.4.2.10</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">pyrE</name>
        <name evidence="6" type="ordered locus">Metig_1633</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>diphosphate + orotidine 5'-phosphate = 5-phospho-alpha-D-ribose 1-diphosphate + orotate</text>
            <dbReference type="Rhea" id="RHEA:10380"/>
            <dbReference type="ChEBI" id="CHEBI:30839"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57538"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="EC" id="2.4.2.10"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1 4">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from orotate: step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.10" evidence="2 4"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97166.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799758.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1633"/>
    <dbReference type="EnsemblBacteria" id="AEF97166">
        <property type="protein sequence ID" value="AEF97166"/>
        <property type="gene ID" value="Metig_1633"/>
    </dbReference>
    <dbReference type="GeneID" id="10644506"/>
    <dbReference type="KEGG" id="mig:Metig_1633"/>
    <dbReference type="HOGENOM" id="CLU_074878_2_0_2"/>
    <dbReference type="OMA" id="MKAYQRQ"/>
    <dbReference type="OrthoDB" id="117225at2157"/>
    <dbReference type="UniPathway" id="UPA00070">
        <property type="reaction ID" value="UER00119"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004588">
        <property type="term" value="F:orotate phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044205">
        <property type="term" value="P:'de novo' UMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01208">
        <property type="entry name" value="PyrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023031">
        <property type="entry name" value="OPRT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004467">
        <property type="entry name" value="Or_phspho_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRibTrfase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00156">
        <property type="entry name" value="Pribosyltran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="SSF53271"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00336">
        <property type="entry name" value="pyrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="4" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3 4" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Pribosyltran" evidence="5">
        <location>
            <begin position="41"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="region of interest" description="5-phosphoribose 1-diphosphate binding" evidence="4">
        <location>
            <begin position="117"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="91"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="92"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="95"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="121"/>
        </location>
        <ligand>
            <name>orotate</name>
            <dbReference type="ChEBI" id="CHEBI:30839"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="149"/>
        </location>
        <ligand>
            <name>orotate</name>
            <dbReference type="ChEBI" id="CHEBI:30839"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEF97166.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="178" mass="19664" checksum="123467839361F06F" modified="2011-07-27" version="1">MEELRRELIELLKEVGCIKFGEFILASGKKSNYYIDIKKATTNPKVLKCIAKLIKEYIKNEDVKVAGVELGSVPIATAVSIETDKELLIIRKKPKDYGTKNKIEGELNKGDKVVVVEDVTTTGGSVIKAVNEIRENGGIVEKVFVVVDRLEGAKENLKNIGVELIPLVTIEDLGVKRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-06-02" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBH4</accession>
    <name>F6BBH4_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Class III signal peptide-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_1649</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97181.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799772.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF97181">
        <property type="protein sequence ID" value="AEF97181"/>
        <property type="gene ID" value="Metig_1649"/>
    </dbReference>
    <dbReference type="GeneID" id="10644522"/>
    <dbReference type="KEGG" id="mig:Metig_1649"/>
    <dbReference type="HOGENOM" id="CLU_197251_0_0_2"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007166">
        <property type="entry name" value="Class3_signal_pept_motif"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04021">
        <property type="entry name" value="Class_IIIsignal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF97181.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="70" mass="7585" checksum="578B3818AD91AE8B" modified="2011-07-27" version="1">MKIITSKKAQITMELGILLMVAIVVAVVAAYHYLTTIKTTGKTVNKTVTNVSSTYSEKIKDLANQLGNLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBK6</accession>
    <name>F6BBK6_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">von Willebrand factor type A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0459</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96015.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798624.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0459"/>
    <dbReference type="EnsemblBacteria" id="AEF96015">
        <property type="protein sequence ID" value="AEF96015"/>
        <property type="gene ID" value="Metig_0459"/>
    </dbReference>
    <dbReference type="GeneID" id="10643296"/>
    <dbReference type="KEGG" id="mig:Metig_0459"/>
    <dbReference type="HOGENOM" id="CLU_036888_0_0_2"/>
    <dbReference type="OMA" id="MTQKITW"/>
    <dbReference type="OrthoDB" id="13269at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="SMART" id="SM00327">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="SSF53300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VWFA" evidence="1">
        <location>
            <begin position="293"/>
            <end position="454"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96015.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="454" mass="52515" checksum="5E50BD9D8A6BE793" modified="2011-07-27" version="1">MKNVIVHDAYDKKAFESFLKNNRYLKALLRNYSQFSPLHEKLAEDTFYAFFKYVVEFNEVVEEEFKINKIILESGMKSIEYEKTKLMTELDEVNAGMATNMFLERFFENIKSLKLQNVYKELAERAKNKEKGNSEGISLDSEGENAELDEFVSKIKDVAKNSLKEISEDIEDTINGMNAVSSWGSGKGNKNALNPEERIKLASKILENKNLHKIIKKLGKLKFIAINEYKSKIKHYSGEIYSIKIGDDLKHLLPKELVNFSDEVLYLDFLRKFVDKKLLTYDLDNEVKKNKGPIIVLLDHSGSMFGEREIWGKAVALSIIEIAKREKRKFGYIAFDDDVRFKRIFEDISIDDILELASLYYGGGTNFELPLECAMEMIEEGFDNADVVLITDGYAEVSDEFLKKLDDFKKDKKLKLISIFIETYPTETIKKISDEFVKVFDLTDEEAKKVFRKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBK9</accession>
    <name>F6BBK9_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Cobyrinic acid ac-diamide synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0462</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96018.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798627.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0462"/>
    <dbReference type="EnsemblBacteria" id="AEF96018">
        <property type="protein sequence ID" value="AEF96018"/>
        <property type="gene ID" value="Metig_0462"/>
    </dbReference>
    <dbReference type="GeneID" id="10643299"/>
    <dbReference type="KEGG" id="mig:Metig_0462"/>
    <dbReference type="HOGENOM" id="CLU_067767_0_0_2"/>
    <dbReference type="OrthoDB" id="50469at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002586">
        <property type="entry name" value="CobQ/CobB/MinD/ParA_Nub-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01656">
        <property type="entry name" value="CbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CbiA" evidence="1">
        <location>
            <begin position="3"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96018.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="264" mass="29768" checksum="B350FE08AC3C20CB" modified="2011-07-27" version="1">MIVAITGGKGGTGKSTLSANLFFYFMENYSTALVDCDVETPNLEYLVSEDLKFAKEVFIEIPDIREGISYNYNNVCKEGALLKINDKLIFIEELCSGCKACGIKTNIKFKKKSIGKIYEKKFDGSYLIVGKSNIGITKTSKIVDETKKYALSKNCEINIIDTAAGIHCNVVRALINSDKVLVVTEPTPFGISDAKRIIKIIEKLKIPYKIVLNRYGINNLNVEYDFKIPYDKRIVETYCKGEGFLKYSDLKKDIEEIANWIIWG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBL5</accession>
    <name>F6BBL5_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">4Fe-4S ferredoxin iron-sulfur binding domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0468</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96024.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798632.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0468"/>
    <dbReference type="EnsemblBacteria" id="AEF96024">
        <property type="protein sequence ID" value="AEF96024"/>
        <property type="gene ID" value="Metig_0468"/>
    </dbReference>
    <dbReference type="GeneID" id="10643305"/>
    <dbReference type="KEGG" id="mig:Metig_0468"/>
    <dbReference type="HOGENOM" id="CLU_145181_0_0_2"/>
    <dbReference type="OMA" id="LCASCIF"/>
    <dbReference type="OrthoDB" id="91206at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00037">
        <property type="entry name" value="Fer4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="116"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96024.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="145" mass="16342" checksum="8A7821B78AB6BD28" modified="2011-07-27" version="1">MPEHILSGIKALVAMKMRKEGKLQKEIAEYLNMDRSIVSHYLHGRYPSDRVMKVSEVIVELPVEYGARIIHSLSNDKYIAKKLIKVIYNAKIKIEKDKCLACGACLGCEAISIEGIEVKINENRCVLCGECISKCPMNALKFVEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBM3</accession>
    <name>F6BBM3_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 12">GMP synthase [glutamine-hydrolyzing] subunit B</fullName>
            <ecNumber evidence="5 12">6.3.5.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11 12">GMP synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">guaAB</name>
        <name evidence="15" type="ordered locus">Metig_0476</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 12">Catalyzes the synthesis of GMP from XMP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 12">
            <text>ATP + H2O + L-glutamine + XMP = AMP + diphosphate + GMP + 2 H(+) + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:11680"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57464"/>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.5.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 12">Purine metabolism; GMP biosynthesis; GMP from XMP (L-Gln route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4 12">Heterodimer composed of a glutamine amidotransferase subunit (A) and a GMP-binding subunit (B).</text>
    </comment>
    <dbReference type="EC" id="6.3.5.2" evidence="5 12"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96032.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798640.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0476"/>
    <dbReference type="EnsemblBacteria" id="AEF96032">
        <property type="protein sequence ID" value="AEF96032"/>
        <property type="gene ID" value="Metig_0476"/>
    </dbReference>
    <dbReference type="GeneID" id="10643313"/>
    <dbReference type="KEGG" id="mig:Metig_0476"/>
    <dbReference type="HOGENOM" id="CLU_014340_0_0_2"/>
    <dbReference type="OMA" id="EGGIKSH"/>
    <dbReference type="OrthoDB" id="31932at2157"/>
    <dbReference type="UniPathway" id="UPA00189">
        <property type="reaction ID" value="UER00296"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003922">
        <property type="term" value="F:GMP synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016462">
        <property type="term" value="F:pyrophosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01997">
        <property type="entry name" value="GMP_synthase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00345">
        <property type="entry name" value="GMP_synthase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001674">
        <property type="entry name" value="GMP_synth_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026598">
        <property type="entry name" value="GMP_synthase_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025777">
        <property type="entry name" value="GMPS_ATP_PPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022310">
        <property type="entry name" value="NAD/GMP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00958">
        <property type="entry name" value="GMP_synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02540">
        <property type="entry name" value="NAD_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00884">
        <property type="entry name" value="guaA_Cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51553">
        <property type="entry name" value="GMPS_ATP_PPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 12 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 12 13" id="KW-0332">GMP biosynthesis</keyword>
    <keyword evidence="12 15" id="KW-0436">Ligase</keyword>
    <keyword evidence="7 12 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9 12 13" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GMPS ATP-PPase" evidence="14">
        <location>
            <begin position="2"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="29"/>
            <end position="35"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00030464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="AEF96032.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="310" mass="34791" checksum="A97BF7286C0FD8E5" modified="2011-07-27" version="1">MFDPKKFIEESIKEIREQIGNRKAIIALSGGVDSSVAAVLTHKAIGDRLLAVFVDTGLMRKGEPEEVTKIFRDQLGLNFKCIDAKDRFLNALKGVTDPEEKRKIIGRVFIEVFEEVARENGEEVLVQGTIAPDWIESEGKIKTHHNIALPGGMVLEVVEPLRDLYKDEVRLVAKELGLPDKIVHRQPFPGPGLAVRILGEITEEKLNICREANAIVEEEIEKAGLDKELWQYFAAVLDTKATGVKGDIRDYNWIVALRFVKSIDAMTAHVPELPWDVLKRISKRITSEIPNVARVVLDITDKPPATIEFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBN6</accession>
    <name>F6BBN6_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Heat shock protein Hsp20</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metig_0489</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the small heat shock protein (HSP20) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96045.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798653.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0489"/>
    <dbReference type="EnsemblBacteria" id="AEF96045">
        <property type="protein sequence ID" value="AEF96045"/>
        <property type="gene ID" value="Metig_0489"/>
    </dbReference>
    <dbReference type="GeneID" id="10643326"/>
    <dbReference type="KEGG" id="mig:Metig_0489"/>
    <dbReference type="HOGENOM" id="CLU_046737_12_4_2"/>
    <dbReference type="OMA" id="SGKGFMP"/>
    <dbReference type="OrthoDB" id="100198at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002068">
        <property type="entry name" value="A-crystallin/Hsp20_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008978">
        <property type="entry name" value="HSP20-like_chaperone"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00011">
        <property type="entry name" value="HSP20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49764">
        <property type="entry name" value="SSF49764"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01031">
        <property type="entry name" value="SHSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0346">Stress response</keyword>
    <feature type="domain" description="SHSP" evidence="3">
        <location>
            <begin position="38"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS01031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEF96045.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="149" mass="16558" checksum="47CAB65C1AA6E6A4" modified="2011-07-27" version="1">MFGRDPFAEIEKMMMEMFMTPFTTSRMSYRMGGGTSIEISGKGFMPVAIIEGDEHIKVVAMLPGVNKEDIVLNAVGDTLEIRAKKAPLMITESERVVYSEIPEDEELYRTIKLPAAVKEENASAKFENGLLIVTLPKAEKSVKKGINIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBN7</accession>
    <name>F6BBN7_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">50S ribosomal protein L14e</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">rpl14e</name>
        <name evidence="4" type="ordered locus">Metig_0490</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the eukaryotic ribosomal protein eL14 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96046.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798654.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0490"/>
    <dbReference type="EnsemblBacteria" id="AEF96046">
        <property type="protein sequence ID" value="AEF96046"/>
        <property type="gene ID" value="Metig_0490"/>
    </dbReference>
    <dbReference type="GeneID" id="10643327"/>
    <dbReference type="KEGG" id="mig:Metig_0490"/>
    <dbReference type="HOGENOM" id="CLU_183474_0_0_2"/>
    <dbReference type="OMA" id="RRCNIKH"/>
    <dbReference type="OrthoDB" id="118643at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06088">
        <property type="entry name" value="KOW_RPL14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00721">
        <property type="entry name" value="Ribosomal_L14e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_L2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023651">
        <property type="entry name" value="Ribosomal_L14e"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039660">
        <property type="entry name" value="Ribosomal_protein_L14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041985">
        <property type="entry name" value="RPL14_KOW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11127">
        <property type="entry name" value="PTHR11127"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="SSF50104"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="1 3 4" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEF96046.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="79" mass="8775" checksum="6517AB71F705ACD3" modified="2011-07-27" version="1">MPAIEIGRVCIKTMGREAGKTCVIVDIIDRNFVLIDGEVKRRRCNIKHIEPTDKKVDIEKGASTEEVRLALDAAGLLKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBN8</accession>
    <name>F6BBN8_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Cytidylate kinase</fullName>
            <shortName evidence="5">CK</shortName>
            <ecNumber evidence="5">2.7.4.25</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Cytidine monophosphate kinase</fullName>
            <shortName evidence="5">CMP kinase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">cmk</name>
        <name evidence="6" type="ordered locus">Metig_0491</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 5">
            <text>ATP + CMP = ADP + CDP</text>
            <dbReference type="Rhea" id="RHEA:11600"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58069"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.25"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 5">
            <text>ATP + dCMP = ADP + dCDP</text>
            <dbReference type="Rhea" id="RHEA:25094"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57566"/>
            <dbReference type="ChEBI" id="CHEBI:58593"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.25"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3 5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 5">Belongs to the cytidylate kinase family. Type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.25" evidence="5"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96047.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798655.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0491"/>
    <dbReference type="EnsemblBacteria" id="AEF96047">
        <property type="protein sequence ID" value="AEF96047"/>
        <property type="gene ID" value="Metig_0491"/>
    </dbReference>
    <dbReference type="GeneID" id="10643328"/>
    <dbReference type="KEGG" id="mig:Metig_0491"/>
    <dbReference type="HOGENOM" id="CLU_079959_1_0_2"/>
    <dbReference type="OMA" id="FIFRDMA"/>
    <dbReference type="OrthoDB" id="110333at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036430">
        <property type="term" value="F:CMP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036431">
        <property type="term" value="F:dCMP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006220">
        <property type="term" value="P:pyrimidine nucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02020">
        <property type="entry name" value="CMPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00239">
        <property type="entry name" value="Cytidyl_kinase_type2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011892">
        <property type="entry name" value="Cyt_kin_arch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011994">
        <property type="entry name" value="Cytidylate_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02173">
        <property type="entry name" value="cyt_kin_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="7"/>
            <end position="15"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEF96047.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="178" mass="20499" checksum="B3E9B6EF5197A281" modified="2011-07-27" version="1">MIITIGGLPGTGTTTMAKKIAEKYGLKHVCAGFIFRDMAKEMGMDLSEFSKYAEENEEVDKEIDRRQVEMAKEGNIVLEGRLAAWMLMRNNIKIDLAIWLKAPPMVRAERISIRENEDVEEAMRKMLEREQSEKKRYKEIYNIDIDDLSIYDLVIDTSKWGVDGVFNIICTAIDNLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBN9</accession>
    <name>F6BBN9_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">50S ribosomal protein L34e</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">rpl34e</name>
        <name evidence="5" type="ordered locus">Metig_0492</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein eL34 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96048.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798656.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0492"/>
    <dbReference type="EnsemblBacteria" id="AEF96048">
        <property type="protein sequence ID" value="AEF96048"/>
        <property type="gene ID" value="Metig_0492"/>
    </dbReference>
    <dbReference type="GeneID" id="10643329"/>
    <dbReference type="KEGG" id="mig:Metig_0492"/>
    <dbReference type="HOGENOM" id="CLU_118652_2_0_2"/>
    <dbReference type="OMA" id="SWRRKYV"/>
    <dbReference type="OrthoDB" id="119402at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00349">
        <property type="entry name" value="Ribosomal_L34e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008195">
        <property type="entry name" value="Ribosomal_L34Ae"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10759">
        <property type="entry name" value="PTHR10759"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01199">
        <property type="entry name" value="Ribosomal_L34e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01250">
        <property type="entry name" value="RIBOSOMALL34"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 5" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009875"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEF96048.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="94" mass="10980" checksum="13D2B428E63FE661" modified="2011-07-27" version="1">MPAPRYRSSSYRKVYRRVPGNRTVIHYRRKKPAKHKCAVCGNILNGVPRGRPVEIRKLAKTEKRPERPYGGYLCPKCLRRLMIQKARSMSNITL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2020-06-17" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBP0</accession>
    <name>F6BBP0_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_0493</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96049.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96049">
        <property type="protein sequence ID" value="AEF96049"/>
        <property type="gene ID" value="Metig_0493"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0493"/>
    <dbReference type="HOGENOM" id="CLU_3194503_0_0_2"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF96049.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="45" mass="5314" checksum="7AA3E4A5030DED20" modified="2011-07-27" version="1">MSPKELINNILIHKFKYIYDNFEYKIMKIENNQNNIKINGAGEGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-06-02" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBQ6</accession>
    <name>F6BBQ6_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_0509</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96065.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798673.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96065">
        <property type="protein sequence ID" value="AEF96065"/>
        <property type="gene ID" value="Metig_0509"/>
    </dbReference>
    <dbReference type="GeneID" id="10643346"/>
    <dbReference type="KEGG" id="mig:Metig_0509"/>
    <dbReference type="HOGENOM" id="CLU_1639973_0_0_2"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012334">
        <property type="entry name" value="Pectin_lyas_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="SSF51126"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF96065.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="161" mass="18104" checksum="269C16193680308B" modified="2011-07-27" version="1">MLSYHPTAIKLNNARDVVLRNVIIKNFPVGIVAENSQVSMSRVYLHNNDIGLDVHSSNLTIANSQFSNRGADIVIKGKSRVEIIDTVVRKILNIIGTSSQLDNEALNIGYLAHKILTTTDANEKEHLWSKIKHYIRTTIIKGEYIADWIIRIKGLIEIFGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-06-02" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBT1</accession>
    <name>F6BBT1_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Class III signal peptide-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_1679</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97211.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799802.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF97211">
        <property type="protein sequence ID" value="AEF97211"/>
        <property type="gene ID" value="Metig_1679"/>
    </dbReference>
    <dbReference type="GeneID" id="10644552"/>
    <dbReference type="KEGG" id="mig:Metig_1679"/>
    <dbReference type="HOGENOM" id="CLU_197251_2_0_2"/>
    <dbReference type="OMA" id="NTHNTAM"/>
    <dbReference type="OrthoDB" id="143954at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007166">
        <property type="entry name" value="Class3_signal_pept_motif"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04021">
        <property type="entry name" value="Class_IIIsignal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF97211.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="67" mass="7316" checksum="8BA63E3E04F0DABF" modified="2011-07-27" version="1">MLKKVKKLIHSTRGQISLEFSILMAAIVSAAVIAGYYIIASSKDVGHNNIDAINHTYNVTMKALNKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBT3</accession>
    <name>F6BBT3_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Radical SAM core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Metig_1681</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97213.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799804.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1681"/>
    <dbReference type="EnsemblBacteria" id="AEF97213">
        <property type="protein sequence ID" value="AEF97213"/>
        <property type="gene ID" value="Metig_1681"/>
    </dbReference>
    <dbReference type="GeneID" id="10644554"/>
    <dbReference type="KEGG" id="mig:Metig_1681"/>
    <dbReference type="HOGENOM" id="CLU_060488_0_0_2"/>
    <dbReference type="OMA" id="KRGPHNC"/>
    <dbReference type="OrthoDB" id="29724at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039661">
        <property type="entry name" value="ELP3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaB/NifB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005909">
        <property type="entry name" value="RaSEA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11135">
        <property type="entry name" value="PTHR11135"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004954">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01210">
        <property type="entry name" value="TIGR01210"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="7">
        <location>
            <begin position="42"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEF97213.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="359" mass="41406" checksum="DA5CF95A8B8BEA94" modified="2011-07-27" version="1">MIENYSELFLKKMREKHLKKRKESNPNKPIATWVQDDIFLDKSIGKSLTIILRTVGCRWAYESGGCTMCSYLMDSSPTPISAENLKKQFDYAIEKVKDLDEFSVKIFTSGSFLDEFEVPREAKEYIFERLGKLEEEGKLKEIAIESRPEFITEENLKNIREYIKDINVEIGVGVETFNEEIRNIAIHKGVTTEDIINAINIAKKYNVGIKAYLLIKPLFITEKDAILDAINSANKAFEVGCSRVSFCPATVHKGTLMEYFWSKNQYRPPFLWSIIEILKEVKSKNPDKIVMCDTSGIPTKRGAHNLYGCECNYKIKNAIEKFTLTQDIDVLDVECKCRKYWEGFIEMEEKNIVPLGDKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2020-08-12" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBV3</accession>
    <name>F6BBV3_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_1701</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97233.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF97233">
        <property type="protein sequence ID" value="AEF97233"/>
        <property type="gene ID" value="Metig_1701"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_1701"/>
    <dbReference type="HOGENOM" id="CLU_210692_0_0_2"/>
    <dbReference type="OMA" id="GMDWKEI"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF97233.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="58" mass="7056" checksum="D7902EE6516C697E" modified="2011-07-27" version="1">MATLLEEGICIIKDKEVAEKLLNDLKSKHTDWREIKKRAERFRKKLEEGERWLEKMEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBW4</accession>
    <name>F6BBW4_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SAM_MT_TRM5_TYW2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metig_1712</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97244.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799833.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1712"/>
    <dbReference type="EnsemblBacteria" id="AEF97244">
        <property type="protein sequence ID" value="AEF97244"/>
        <property type="gene ID" value="Metig_1712"/>
    </dbReference>
    <dbReference type="GeneID" id="10644586"/>
    <dbReference type="KEGG" id="mig:Metig_1712"/>
    <dbReference type="HOGENOM" id="CLU_022610_0_1_2"/>
    <dbReference type="OMA" id="HYYDIQH"/>
    <dbReference type="OrthoDB" id="55502at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006400">
        <property type="term" value="P:tRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030382">
        <property type="entry name" value="MeTrfase_TRM5/TYW2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040601">
        <property type="entry name" value="Trm5_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02475">
        <property type="entry name" value="Met_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18093">
        <property type="entry name" value="Trm5_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51684">
        <property type="entry name" value="SAM_MT_TRM5_TYW2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SAM_MT_TRM5_TYW2" evidence="3">
        <location>
            <begin position="98"/>
            <end position="336"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEF97244.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="337" mass="38993" checksum="E177117A54BEAB6D" modified="2011-07-27" version="1">MILCAKVHIKEGEKTRRLLLENKLLNKNYKIVKEGNFLYIPLNEENLKNVNLKDLNPNIEIVEKDLKSKEIKRKPSFREVISKRFRKEIDEGLIALSYDVVGDLVILQISDEIDEKTRKEIGEIAYKLIPCKGVFRRKSEVKGEFRVRELEHLAGENRTLTMHKENGYRLWVDIAKVYFSPRLGGERLRIGKKVGIDDVVVDMFAGVGPFSIACRNARKIYSIDINPHAIELLKKNIKLNKLEHKIIPILSDVREVDVKGNRVIMNLPKYAHEFVDKALEIVEKGGVVHYYTIGEDFDDAIKLFESKCECEVLEKKIVKSYAPKEYVLAIDFKILEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-06-02" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBY2</accession>
    <name>F6BBY2_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_1730</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97262.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF97262">
        <property type="protein sequence ID" value="AEF97262"/>
        <property type="gene ID" value="Metig_1730"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_1730"/>
    <dbReference type="HOGENOM" id="CLU_175324_2_1_2"/>
    <dbReference type="OMA" id="KMAIGWL"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019707">
        <property type="entry name" value="DUF2582"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10771">
        <property type="entry name" value="DUF2582"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF97262.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="67" mass="7765" checksum="814913815225E322" modified="2011-07-27" version="1">MWAKIGETAGRVYHLLEEGEKNLSSLTKILRREGHNTNLVIMAIGWLAREDKINVIKDHGKWIIRLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>F6BC74</accession>
    <name>F6BC74_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="1 7">16S rRNA aminocarboxypropyltransferase</fullName>
            <ecNumber evidence="7">2.5.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Metig_1748</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Aminocarboxypropyltransferase that catalyzes the aminocarboxypropyl transfer on pseudouridine corresponding to position 914 in M.jannaschii 16S rRNA. It constitutes the last step in biosynthesis of the hypermodified N1-methyl-N3-(3-amino-3-carboxypropyl) pseudouridine (m1acp3-Psi).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>an N(1)-methylpseudouridine in 16S/18S rRNA + S-adenosyl-L-methionine = H(+) + N(1)-methyl-N(3)[(3S)-3-amino-3-carboxypropyl]pseudouridine in 16S/18S rRNA + S-methyl-5'-thioadenosine</text>
            <dbReference type="Rhea" id="RHEA:63296"/>
            <dbReference type="Rhea" id="RHEA-COMP:11634"/>
            <dbReference type="Rhea" id="RHEA-COMP:16310"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17509"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74890"/>
            <dbReference type="ChEBI" id="CHEBI:146234"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the TDD superfamily. TSR3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.-" evidence="7"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97280.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1748"/>
    <dbReference type="EnsemblBacteria" id="AEF97280">
        <property type="protein sequence ID" value="AEF97280"/>
        <property type="gene ID" value="Metig_1748"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_1748"/>
    <dbReference type="HOGENOM" id="CLU_035060_4_2_2"/>
    <dbReference type="OMA" id="DCSWESA"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106388">
        <property type="term" value="F:18S rRNA aminocarboxypropyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904047">
        <property type="term" value="F:S-adenosyl-L-methionine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000455">
        <property type="term" value="P:enzyme-directed rRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01116">
        <property type="entry name" value="TSR3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007209">
        <property type="entry name" value="RNaseL-inhib-like_metal-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022968">
        <property type="entry name" value="Tsr3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007177">
        <property type="entry name" value="Tsr3_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20426">
        <property type="entry name" value="PTHR20426"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04034">
        <property type="entry name" value="Ribo_biogen_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04068">
        <property type="entry name" value="RLI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="4 7" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="6 7" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="RLI" evidence="9">
        <location>
            <begin position="2"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="domain" description="Ribo_biogen_C" evidence="8">
        <location>
            <begin position="42"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="18"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="68"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="90"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="109"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00014114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF04068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AEF97280.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="171" mass="19515" checksum="489EDBF14F8948CF" modified="2011-07-27" version="1">MVKLYIYHANQCNPKKCTALKMGKLGKAIILKNPYKIPKNSILLNPYAEKSVSVEDRPIIEKYGVTALDCSWKEAEKIFKKVSTKNQRSLPFLIAANPINYGKPCQLSTLEAFVATLYIANYKNDALSLLNGFKWAETFIKINEELLERYANAKNSKEIVDIQNEYLSKFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F6BC76</accession>
    <name>F6BC76_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="3">ATP-grasp fold domain protein, DUF201-type</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_1750</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97282.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799871.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1750"/>
    <dbReference type="EnsemblBacteria" id="AEF97282">
        <property type="protein sequence ID" value="AEF97282"/>
        <property type="gene ID" value="Metig_1750"/>
    </dbReference>
    <dbReference type="GeneID" id="10644624"/>
    <dbReference type="KEGG" id="mig:Metig_1750"/>
    <dbReference type="HOGENOM" id="CLU_059501_1_1_2"/>
    <dbReference type="OMA" id="DVNPRPT"/>
    <dbReference type="OrthoDB" id="58527at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003806">
        <property type="entry name" value="ATP-grasp_PylC-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005479">
        <property type="entry name" value="CbamoylP_synth_lsu-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024710">
        <property type="entry name" value="MfnD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040803">
        <property type="entry name" value="MfnD_preATP-grasp"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02655">
        <property type="entry name" value="ATP-grasp_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18301">
        <property type="entry name" value="preATP-grasp_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016766">
        <property type="entry name" value="UCP016766_ATPgrasp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00867">
        <property type="entry name" value="CPSASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-grasp" evidence="2">
        <location>
            <begin position="208"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF97282.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="299" mass="33566" checksum="2AA8949CAE1CA02D" modified="2011-07-27" version="1">MIFLYEFAVGGGAIEGIPKDILCEGKAMFDTLLNQFLEFDDVITLIDGRFYEDYKKKRNLHINTIKEGDDLTEKIEDILKLSDMALIIAPEDDGILYNLTKIVEDSGVLNLGSSSDAIKIAGDKYLTYKSIKDVVNTPKTMPLKKYVIKKIDGCGGEHFIYDENYIVQEFVEGEPYSVSLIVGDEIYPLSLNKQYINANGYCGGEINISHPLKEEIFRECIKAVEQIDGLKGYIGVDVIVGDEIYIIEINPRITTSIYGLKTNPSLAKLLVDNALGKELKFKVEEGRKFIKKDGKMSFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>F6BC83</accession>
    <name>F6BC83_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Cobyrinic acid ac-diamide synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1757</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97289.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799878.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1757"/>
    <dbReference type="EnsemblBacteria" id="AEF97289">
        <property type="protein sequence ID" value="AEF97289"/>
        <property type="gene ID" value="Metig_1757"/>
    </dbReference>
    <dbReference type="GeneID" id="10644631"/>
    <dbReference type="KEGG" id="mig:Metig_1757"/>
    <dbReference type="HOGENOM" id="CLU_082962_0_0_2"/>
    <dbReference type="OMA" id="GCVCPAS"/>
    <dbReference type="OrthoDB" id="61135at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002586">
        <property type="entry name" value="CobQ/CobB/MinD/ParA_Nub-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014433">
        <property type="entry name" value="CooC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01656">
        <property type="entry name" value="CbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005647">
        <property type="entry name" value="CooC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CbiA" evidence="1">
        <location>
            <begin position="4"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF97289.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="259" mass="28591" checksum="859D5B1CB27B7DCD" modified="2011-07-27" version="1">MKIAITGKGGVGKTFISSVLARLFEKSGYKVIAVDADPNTTLACALGIDEEITPISKMNDLIEERTGAKPNSYGAVFKLNPKVDDIVDKFAYKKGNIYLLVMGTVEKGGSGCVCPASVLLRRLLQHLILKRDEVVILDMEAGIEHLGRKTTENVDLMIVVIEPSKKSLLTAKRIKKLANDIGVNNLAVIVNKIRNIEDKELIKNLIEREVGLPVIGFIPYDEEVLKSDFLGKPVDLNSKSAKEVEKIFNYILKLKQNQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCA9</accession>
    <name>F6BCA9_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Type II methyltransferase</fullName>
            <ecNumber evidence="7">2.1.1.113</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">N-4 cytosine-specific methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Metig_1783</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>a 2'-deoxycytidine in DNA + S-adenosyl-L-methionine = an N(4)-methyl-2'-deoxycytidine in DNA + H(+) + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:16857"/>
            <dbReference type="Rhea" id="RHEA-COMP:11369"/>
            <dbReference type="Rhea" id="RHEA-COMP:13674"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:85452"/>
            <dbReference type="ChEBI" id="CHEBI:137933"/>
            <dbReference type="EC" id="2.1.1.113"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the N(4)/N(6)-methyltransferase family. N(4) subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.113" evidence="7"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97315.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799904.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="REBASE" id="36265">
        <property type="entry name" value="M.Mig5ORF1783P"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF97315">
        <property type="protein sequence ID" value="AEF97315"/>
        <property type="gene ID" value="Metig_1783"/>
    </dbReference>
    <dbReference type="GeneID" id="10644660"/>
    <dbReference type="KEGG" id="mig:Metig_1783"/>
    <dbReference type="HOGENOM" id="CLU_024927_2_3_2"/>
    <dbReference type="OMA" id="LWNFSGE"/>
    <dbReference type="OrthoDB" id="63009at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008170">
        <property type="term" value="F:N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015667">
        <property type="term" value="F:site-specific DNA-methyltransferase (cytosine-N4-specific) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009307">
        <property type="term" value="P:DNA restriction-modification system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002941">
        <property type="entry name" value="DNA_methylase_N4/N6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017985">
        <property type="entry name" value="MeTrfase_CN4_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001091">
        <property type="entry name" value="RM_Methyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01555">
        <property type="entry name" value="N6_N4_Mtase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00508">
        <property type="entry name" value="S21N4MTFRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00093">
        <property type="entry name" value="N4_MTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7 9" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 7" id="KW-0680">Restriction system</keyword>
    <keyword evidence="5 7" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N6_N4_Mtase" evidence="8">
        <location>
            <begin position="27"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU362026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AEF97315.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="258" mass="29599" checksum="169E6ECEE761EFA8" modified="2011-07-27" version="1">MEVWNKIRGIKVINDDFLNVDLPEESIDLIVTSPPYNVGIDYNQHDDNIPYEEYLNWTKQWLKKALTLLKKDGRLCLNIPLDKNKGGIKPVYADIVKIALDVGFKYQSTIIWNEQNISRRTAWGSWLSASAPYVIAPVETIVVFYKETWKKLSKGKSDITREEFIEWTNGLWTFPGESKKRVGHPAPFPLELPKRCIKLFSYVGDVVLDPFLGSGTTVIAAYKLGRRAIGVEIDRKYFELAIKRIANECCTLEGLLWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCC4</accession>
    <name>F6BCC4_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Putative nickel insertion protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_1798</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the LarC family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97330.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1798"/>
    <dbReference type="EnsemblBacteria" id="AEF97330">
        <property type="protein sequence ID" value="AEF97330"/>
        <property type="gene ID" value="Metig_1798"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_1798"/>
    <dbReference type="HOGENOM" id="CLU_028523_2_1_2"/>
    <dbReference type="OMA" id="EILCKHE"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016151">
        <property type="term" value="F:nickel cation binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01074">
        <property type="entry name" value="LarC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002822">
        <property type="entry name" value="Ni_insertion"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36566">
        <property type="entry name" value="PTHR36566"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01969">
        <property type="entry name" value="DUF111"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00299">
        <property type="entry name" value="TIGR00299"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0456">Lyase</keyword>
    <keyword evidence="1 2" id="KW-0533">Nickel</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF97330.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="379" mass="43071" checksum="E875174D414E6293" modified="2011-07-27" version="1">MIILNPFMGISGDMFLSAMVDFVDEEGLINTIKKVVDVDIEVKKVKKRGIIANKINIIPKEKENLENRNYKWIKKLIKNSSIDDNIKKHALGMFKILAEAEAKVHGIDVEKVHFHEIGQVDTIADIVGAAYCIEKLKNEEFYYTPINVGSGFVNTMHGKMPVPAPATLEILKGFEIFFSEYGELTTPTGATIIKYLNPKLAKSTFNVEKISYGAGDKDFEAPNVLRVIKAKDNFKDEVCLIETNVDDVSPEILGYLYEVLKDKVRDLHFIPCFMKKNRPAYIIRIIANAKDIDEICEILMQETGTIGIRVIPYVHRSIAKREFKTINVFGEDVKVKVSYFNGKIVSKKPEFEDLKKVAKKHNLPLKDVYLEVIKKIKNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCC8</accession>
    <name>F6BCC8_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_1802</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97334.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF97334">
        <property type="protein sequence ID" value="AEF97334"/>
        <property type="gene ID" value="Metig_1802"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_1802"/>
    <dbReference type="HOGENOM" id="CLU_1631659_0_0_2"/>
    <dbReference type="OMA" id="MSMDQEK"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF97334.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="162" mass="19127" checksum="5083C1CEA73F1083" modified="2011-07-27" version="1">MFIEPKVRAAKTPQEFSPKFLTHTTINKIVKEKIGEIRNLKRMRADIINSLKQQNKDGILSNIERLEYILQTRKEAEIIKKIDNYNKLLNKIKKIDELTIKIREKPNEISELLKKSSEEHQKVLENAKISTKIHAELLDIYYEINRLKAKSKELYQLISTKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCE0</accession>
    <name>F6BCE0_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="3">von Willebrand factor type A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_0597</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Mg-chelatase subunits D/I family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96151.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0597"/>
    <dbReference type="EnsemblBacteria" id="AEF96151">
        <property type="protein sequence ID" value="AEF96151"/>
        <property type="gene ID" value="Metig_0597"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0597"/>
    <dbReference type="HOGENOM" id="CLU_016684_2_0_2"/>
    <dbReference type="OMA" id="INMEHAA"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd01451">
        <property type="entry name" value="vWA_Magnesium_chelatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041702">
        <property type="entry name" value="BchD/ChlD_VWA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13519">
        <property type="entry name" value="VWA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00327">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="SSF53300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VWFA" evidence="2">
        <location>
            <begin position="82"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF96151.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="265" mass="30440" checksum="88F2515F93E4AAF2" modified="2011-07-27" version="1">MRDSIYRYSSGRHIKSISKKGRYIKYRIPHGKTTDIAFDATFRVSALYQKERREKFKDKNLALYLTKDDLREKVRERKISSHLLFVVDASGSMGAMRRMEAAKGAVLSLLMDAYQKRNKVGMIAFRKDMAELVLPFTSSVELGEKLLKELPTGGKTPLSKAFLKAYEVFKNEMRKNPNIIPIMIFISDFKPNVAIKKDYIKEVFDICDKIAEEGINVILIDTEPKSFIKIGIGDKIAKRYGFKYYKIDDISVDGILNIVNPLINY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCG7</accession>
    <name>F6BCG7_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 13">V-type ATP synthase alpha chain</fullName>
            <ecNumber evidence="4 13">7.1.2.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12 13">V-ATPase subunit A</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">atpA</name>
        <name evidence="17" type="ordered locus">Metig_0628</name>
    </gene>
    <organism evidence="18">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 13">Produces ATP from ADP in the presence of a proton gradient across the membrane. The archaeal alpha chain is a catalytic subunit.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 13">
            <text>ATP + 4 H(+)(in) + H2O = ADP + 5 H(+)(out) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:57720"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.1.2.2"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the ATPase alpha/beta chains family.</text>
    </comment>
    <dbReference type="EC" id="7.1.2.2" evidence="4 13"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96178.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798783.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0628"/>
    <dbReference type="EnsemblBacteria" id="AEF96178">
        <property type="protein sequence ID" value="AEF96178"/>
        <property type="gene ID" value="Metig_0628"/>
    </dbReference>
    <dbReference type="GeneID" id="10643467"/>
    <dbReference type="KEGG" id="mig:Metig_0628"/>
    <dbReference type="HOGENOM" id="CLU_008162_3_1_2"/>
    <dbReference type="OMA" id="RIVKTFW"/>
    <dbReference type="OrthoDB" id="6736at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033178">
        <property type="term" value="C:proton-transporting two-sector ATPase complex, catalytic domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042777">
        <property type="term" value="P:proton motive force-driven plasma membrane ATP synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1140.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00309">
        <property type="entry name" value="ATP_synth_A_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031686">
        <property type="entry name" value="ATP-synth_a_Xtn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023366">
        <property type="entry name" value="ATP_synth_asu-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005726">
        <property type="entry name" value="ATP_synth_asu_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020003">
        <property type="entry name" value="ATPase_a/bsu_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004100">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036121">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000194">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_nucl-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024034">
        <property type="entry name" value="ATPase_F1/V1_b/a_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022878">
        <property type="entry name" value="V-ATPase_asu"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43607">
        <property type="entry name" value="PTHR43607"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00006">
        <property type="entry name" value="ATP-synt_ab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02874">
        <property type="entry name" value="ATP-synt_ab_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16886">
        <property type="entry name" value="ATP-synt_ab_Xtn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50615">
        <property type="entry name" value="SSF50615"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01043">
        <property type="entry name" value="ATP_syn_A_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00152">
        <property type="entry name" value="ATPASE_ALPHA_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 13" id="KW-0066">ATP synthesis</keyword>
    <keyword evidence="8 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="13" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="10 13" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 13" id="KW-1278">Translocase</keyword>
    <keyword evidence="6 13" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ATP-synt_ab_N" evidence="15">
        <location>
            <begin position="6"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="domain" description="ATP-synt_ab_Xtn" evidence="16">
        <location>
            <begin position="84"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="domain" description="ATP-synt_ab" evidence="14">
        <location>
            <begin position="213"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="233"/>
            <end position="240"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00018003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00031719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF02874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF16886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="AEF96178.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="588" mass="65510" checksum="CCF99DDA72F052B8" modified="2011-07-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCJ5</accession>
    <name>F6BCJ5_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_0656</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96206.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798809.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0656"/>
    <dbReference type="EnsemblBacteria" id="AEF96206">
        <property type="protein sequence ID" value="AEF96206"/>
        <property type="gene ID" value="Metig_0656"/>
    </dbReference>
    <dbReference type="GeneID" id="10643496"/>
    <dbReference type="KEGG" id="mig:Metig_0656"/>
    <dbReference type="HOGENOM" id="CLU_060249_0_0_2"/>
    <dbReference type="OMA" id="HAFTPWT"/>
    <dbReference type="OrthoDB" id="29545at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005287">
        <property type="entry name" value="CHP00375"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016195">
        <property type="entry name" value="Pol/histidinol_Pase-like"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89550">
        <property type="entry name" value="SSF89550"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00375">
        <property type="entry name" value="TIGR00375"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF96206.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="392" mass="45044" checksum="3EEB125E7B2B7C50" modified="2011-07-27" version="1">MIISADLHIHSKYSGGTSKYMDIEHILKYGKLKGLDLIGTGDCLHEKWLNEIKNYKDKNLLLTTEIEDINRVHHLVFLPSISKVEELREILKRYSKNIDDDGRPRVLMDGKELIEVVHDVGGLIGAAHAFTPWTSLYKSFDSVYDCYGKKPDFIELGLSADTDMADMIKELRDIPFLSNSDAHSYHPHRLGREFNQFEVDSLGNLEENFEEIRKAIKNNKIVANYGLDPKLGKYHLTACTKCYLRFKLEDAKRLNFKCPCGGTIKKGVLSRVEELSDGQIIHPKFRPPYYKIIPLAEIISLSTKRGIGTQTVNNLWEKFIEKYKNEINVLINADIEELKKINEDVGNTIELFREGKIYIYPGGGGEYGRIMKTPPKIKWYVMQNTLDAWLNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCQ6</accession>
    <name>F6BCQ6_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0254 protein Metig_0717</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0717</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0254 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96267.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798870.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96267">
        <property type="protein sequence ID" value="AEF96267"/>
        <property type="gene ID" value="Metig_0717"/>
    </dbReference>
    <dbReference type="GeneID" id="10643557"/>
    <dbReference type="KEGG" id="mig:Metig_0717"/>
    <dbReference type="HOGENOM" id="CLU_1451416_0_0_2"/>
    <dbReference type="OMA" id="ECFTHGK"/>
    <dbReference type="OrthoDB" id="81238at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00673">
        <property type="entry name" value="UPF0254"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009625">
        <property type="entry name" value="HcgF"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06787">
        <property type="entry name" value="HcgF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96267.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="176" mass="19985" checksum="72755643EE790273" modified="2011-07-27" version="1">MVDVKISVGTAECFTHAKIGITIHKAASGYKDFEFKEIFDKEELRMMSNVRVLASMFIPSIYAAEKLLNIKLPEPDYDYAYAKAYTEKNDLKVAYLMAKGLKDILNCNIAIGTTAGIGRGGICILTDKNKYLFTTDVYGNLLKHENILERQRNGIEKGLRKFIEILKREYLHTSSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCW2</accession>
    <name>F6BCW2_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Auxin Efflux Carrier</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Metig_0777</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the auxin efflux carrier (TC 2.A.69) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96323.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798926.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0777"/>
    <dbReference type="EnsemblBacteria" id="AEF96323">
        <property type="protein sequence ID" value="AEF96323"/>
        <property type="gene ID" value="Metig_0777"/>
    </dbReference>
    <dbReference type="GeneID" id="10643617"/>
    <dbReference type="KEGG" id="mig:Metig_0777"/>
    <dbReference type="HOGENOM" id="CLU_056175_5_2_2"/>
    <dbReference type="OMA" id="TTFFWTL"/>
    <dbReference type="OrthoDB" id="66562at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004776">
        <property type="entry name" value="Mem_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038770">
        <property type="entry name" value="Na+/solute_symporter_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03547">
        <property type="entry name" value="Mem_trans"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="30"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="61"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="93"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="122"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="157"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="213"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="253"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="279"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEF96323.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="303" mass="33279" checksum="70C0750FCD4E2D89" modified="2011-07-27" version="1">MDVIIIVMNLILIGYLLKYLKLLKETDRIALNNIVVYITMPATIFLTIMTKVSSSDLFEFLKLPFAIIITDLICGILAYFVGKSFLKLNNKSLGGFILVCMLGNTGFLGYPIAYGLYGDDGLARAIFCDMGGVFATMLFGTYVGIKFGKNSGNILKELLRFPPLITGIFTIILVFFGVELDYFPSFIIKTLEYLSSATIPLIMLSLGLSLSPSAAKFGIFYGMLVSLFRFGVSPATAYSLSEVFLISGLEKKVLIIESAMPSALMTLVLSTLYELDVKLVASCCFITTVLSLAVIPIIQYLKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCW6</accession>
    <name>F6BCW6_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="3">CBS domain containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_0781</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96327.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96327">
        <property type="protein sequence ID" value="AEF96327"/>
        <property type="gene ID" value="Metig_0781"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0781"/>
    <dbReference type="HOGENOM" id="CLU_040681_9_3_2"/>
    <dbReference type="OMA" id="TKTDICR"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="SSF54631"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0129">CBS domain</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBS" evidence="2">
        <location>
            <begin position="7"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF96327.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="90" mass="10381" checksum="C47DF34B65B9E9A6" modified="2011-07-27" version="1">MKVKEIMNPDIYKVGENENLYKAFKVMHDRGIRRVFVEKDGKIVGVISYRDLVNVFVSRGLFELIETKVGDIATKNILKIHEDDDIKDAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCY2</accession>
    <name>F6BCY2_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Cation antiporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Metig_0798</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96343.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798946.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0798"/>
    <dbReference type="EnsemblBacteria" id="AEF96343">
        <property type="protein sequence ID" value="AEF96343"/>
        <property type="gene ID" value="Metig_0798"/>
    </dbReference>
    <dbReference type="GeneID" id="10643639"/>
    <dbReference type="KEGG" id="mig:Metig_0798"/>
    <dbReference type="HOGENOM" id="CLU_2257387_0_0_2"/>
    <dbReference type="OMA" id="IIPFEPY"/>
    <dbReference type="OrthoDB" id="107043at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008324">
        <property type="term" value="F:cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002758">
        <property type="entry name" value="Cation_antiport_E"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34584">
        <property type="entry name" value="PTHR34584"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01899">
        <property type="entry name" value="MNHE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEF96343.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="100" mass="11148" checksum="2B95141C383CE8FD" modified="2011-07-27" version="1">MRLFGVIGYIVVLLKAIAEAWVDVVKRCINGDINPQVIEIETSINSLIGQVLLACSITITPGTLTIDLIPEKRILKVATISPRTRDEIIPFEPYIKKIFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F6BD27</accession>
    <name>F6BD27_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 9">Translation initiation factor 2 subunit beta</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7 9">aIF2-beta</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8 9">eIF-2-beta</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">eif2b</name>
        <name evidence="11" type="ordered locus">Metig_0844</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 9">eIF-2 functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 9">Heterotrimer composed of an alpha, a beta and a gamma chain.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the eIF-2-beta/eIF-5 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96388.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798990.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0844"/>
    <dbReference type="EnsemblBacteria" id="AEF96388">
        <property type="protein sequence ID" value="AEF96388"/>
        <property type="gene ID" value="Metig_0844"/>
    </dbReference>
    <dbReference type="GeneID" id="10643685"/>
    <dbReference type="KEGG" id="mig:Metig_0844"/>
    <dbReference type="HOGENOM" id="CLU_026663_3_1_2"/>
    <dbReference type="OMA" id="GKPDTRL"/>
    <dbReference type="OrthoDB" id="96453at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00232">
        <property type="entry name" value="eIF_2_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045196">
        <property type="entry name" value="IF2/IF5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004458">
        <property type="entry name" value="TIF2_bsu_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002735">
        <property type="entry name" value="Transl_init_fac_IF2/IF5_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016189">
        <property type="entry name" value="Transl_init_fac_IF2/IF5_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016190">
        <property type="entry name" value="Transl_init_fac_IF2/IF5_Zn-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23001">
        <property type="entry name" value="PTHR23001"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01873">
        <property type="entry name" value="eIF-5_eIF-2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00653">
        <property type="entry name" value="eIF2B_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100966">
        <property type="entry name" value="SSF100966"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75689">
        <property type="entry name" value="SSF75689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00311">
        <property type="entry name" value="aIF-2beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9 11" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="6 9" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="eIF2B_5" evidence="10">
        <location>
            <begin position="26"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00031466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00032408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AEF96388.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="139" mass="16115" checksum="5A143CA61288C251" modified="2011-07-27" version="1">MSIDYYDYEALLKRAREQLPEDVFKDARFEIPEAEVFVEGSRTIIRNFKEIAKLIDRDVNYFAKYIMKELGTAGDLEGTRLVLQGRFSGYLVNSKIQDFLKEYVLCHECGKPDTKIIKEGRIHMLKCMACGAIRPIKLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>F6BD31</accession>
    <name>F6BD31_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Transcription elongation factor Spt5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">spt5</name>
        <name evidence="6" type="ordered locus">Metig_0848</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Stimulates transcription elongation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Heterodimer composed of Spt4 and Spt5. Interacts with RNA polymerase (RNAP).</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the SPT5 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the archaeal Spt5 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96392.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798994.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0848"/>
    <dbReference type="EnsemblBacteria" id="AEF96392">
        <property type="protein sequence ID" value="AEF96392"/>
        <property type="gene ID" value="Metig_0848"/>
    </dbReference>
    <dbReference type="GeneID" id="10643689"/>
    <dbReference type="KEGG" id="mig:Metig_0848"/>
    <dbReference type="HOGENOM" id="CLU_113589_0_0_2"/>
    <dbReference type="OMA" id="FSEIEHF"/>
    <dbReference type="OrthoDB" id="91936at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.940">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00950">
        <property type="entry name" value="Spt5_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005824">
        <property type="entry name" value="KOW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005100">
        <property type="entry name" value="NGN-domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006645">
        <property type="entry name" value="NGN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036735">
        <property type="entry name" value="NGN_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_L2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011590">
        <property type="entry name" value="Spt5_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00467">
        <property type="entry name" value="KOW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03439">
        <property type="entry name" value="Spt5-NGN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00739">
        <property type="entry name" value="KOW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00738">
        <property type="entry name" value="NGN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="SSF50104"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00405">
        <property type="entry name" value="KOW_elon_Spt5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="NGN" evidence="4">
        <location>
            <begin position="1"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="domain" description="KOW" evidence="5">
        <location>
            <begin position="88"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEF96392.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="146" mass="16196" checksum="A2B34CD92F330F78" modified="2011-07-27" version="1">MIFAVRTTAGQEKNVAEMLASKAERENLDVYSILATENLKGYILVEAENRGVVEELVRGAFKVKGIVPGTTSIDELEHLLTPRKLVEDIEKGDLVELVAGPFKGERARVIRVDKHKEEITIELEQAAVPIPITVNVEHVKIVSKNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>F6BD37</accession>
    <name>F6BD37_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Cobalamin synthesis protein P47K</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0854</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96398.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799000.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0854"/>
    <dbReference type="EnsemblBacteria" id="AEF96398">
        <property type="protein sequence ID" value="AEF96398"/>
        <property type="gene ID" value="Metig_0854"/>
    </dbReference>
    <dbReference type="GeneID" id="10643695"/>
    <dbReference type="KEGG" id="mig:Metig_0854"/>
    <dbReference type="HOGENOM" id="CLU_1193419_0_0_2"/>
    <dbReference type="OMA" id="DMCPDHF"/>
    <dbReference type="OrthoDB" id="56639at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016151">
        <property type="term" value="F:nickel cation binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051604">
        <property type="term" value="P:protein maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003495">
        <property type="entry name" value="CobW/HypB/UreG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004392">
        <property type="entry name" value="Hyd_mat_HypB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30134">
        <property type="entry name" value="PTHR30134"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02492">
        <property type="entry name" value="cobW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="cobW" evidence="1">
        <location>
            <begin position="3"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96398.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="234" mass="25925" checksum="CE93DE109B2B04A8" modified="2011-07-27" version="1">MKIVIVAGTPGAGKTSLLIHTIRHLKDRGCHPAVVKIDCLYTDDDVRYKKLGVPVLVGLSKDMCPDHFAIYNIEEMVEWAKEQNADILIVETAGLCHRCAPYTKNSLGICVIDATCGPNTPRKVGPFLTSADVVAITKGDIVSQAEREVFRERVLEMNPRCRIYDVNGLSGQGSAEIAKEILECKDVENLENEELRHSAPLCICTLCVGETRIAKKFHRGVLRRIDGFMKYVGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>F6BD52</accession>
    <name>F6BD52_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">4Fe-4S ferredoxin iron-sulfur binding domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0869</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96413.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799015.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0869"/>
    <dbReference type="EnsemblBacteria" id="AEF96413">
        <property type="protein sequence ID" value="AEF96413"/>
        <property type="gene ID" value="Metig_0869"/>
    </dbReference>
    <dbReference type="GeneID" id="10643713"/>
    <dbReference type="KEGG" id="mig:Metig_0869"/>
    <dbReference type="HOGENOM" id="CLU_139698_5_5_2"/>
    <dbReference type="OMA" id="CLSCHEI"/>
    <dbReference type="OrthoDB" id="111853at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00037">
        <property type="entry name" value="Fer4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="32"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96413.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="62" mass="6767" checksum="AAA0C723BE443C0A" modified="2011-07-27" version="1">MAVTIDYSKCKGPECAECVNTCPMEVFEIQGDKVVVAKEDECTFCGVCEDVCPTKAIKVTQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>F6BD56</accession>
    <name>F6BD56_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="5">CRISPR-associated protein Cas6</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Metig_0874</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CRISPR-associated protein Cas6/Cse3/CasE family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96417.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799019.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0874"/>
    <dbReference type="EnsemblBacteria" id="AEF96417">
        <property type="protein sequence ID" value="AEF96417"/>
        <property type="gene ID" value="Metig_0874"/>
    </dbReference>
    <dbReference type="GeneID" id="10643718"/>
    <dbReference type="KEGG" id="mig:Metig_0874"/>
    <dbReference type="HOGENOM" id="CLU_089858_1_0_2"/>
    <dbReference type="OMA" id="FGMVKVD"/>
    <dbReference type="OrthoDB" id="59332at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1890">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010156">
        <property type="entry name" value="CRISPR-assoc_prot_Cas6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045747">
        <property type="entry name" value="CRISPR-assoc_prot_Cas6_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36984">
        <property type="entry name" value="PTHR36984"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01881">
        <property type="entry name" value="Cas_Cas6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005054">
        <property type="entry name" value="PF1131"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01877">
        <property type="entry name" value="cas_cas6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0051">Antiviral defense</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Proton acceptor" evidence="4">
        <location>
            <position position="28"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="4">
        <location>
            <position position="43"/>
        </location>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="3">
        <location>
            <position position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR005054-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR005054-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEF96417.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="237" mass="28059" checksum="2577686D7397CCE3" modified="2011-07-27" version="1">MRIELELETEEFTIIPYNHQYYLASALYNKIHSSNPEYAERLHSYQKFKFFTFSLLQIRKRMIKKDGIETIDGKVNLYISSPSVEFLENFISGLLEDGMLKVGDIEFFVKKAKILPTPEKFEVLKTLSPIYLKTLVDTEEGKKTYDLLPNNSKFYENLKKNLKKKYEAFYNKKCDLDFEFEVLKYKPKRMRIKNTYHKCSEMVFRVWGDYELIKFGYDCGFGEKNSMGFGMVQAISL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>F6BD57</accession>
    <name>F6BD57_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Peptidase U62 modulator of DNA gyrase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metig_0875</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96418.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799020.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0875"/>
    <dbReference type="EnsemblBacteria" id="AEF96418">
        <property type="protein sequence ID" value="AEF96418"/>
        <property type="gene ID" value="Metig_0875"/>
    </dbReference>
    <dbReference type="GeneID" id="10643719"/>
    <dbReference type="KEGG" id="mig:Metig_0875"/>
    <dbReference type="HOGENOM" id="CLU_026425_4_2_2"/>
    <dbReference type="OMA" id="LYRKSTF"/>
    <dbReference type="OrthoDB" id="49762at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2290.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045569">
        <property type="entry name" value="Metalloprtase-TldD/E_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045570">
        <property type="entry name" value="Metalloprtase-TldD/E_cen_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002510">
        <property type="entry name" value="Metalloprtase-TldD/E_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035068">
        <property type="entry name" value="TldD/PmbA_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036059">
        <property type="entry name" value="TldD/PmbA_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01523">
        <property type="entry name" value="PmbA_TldD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19289">
        <property type="entry name" value="PmbA_TldD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19290">
        <property type="entry name" value="PmbA_TldD_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111283">
        <property type="entry name" value="SSF111283"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PmbA_TldD" evidence="1">
        <location>
            <begin position="18"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="domain" description="PmbA_TldD_M" evidence="3">
        <location>
            <begin position="106"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="domain" description="PmbA_TldD_C" evidence="2">
        <location>
            <begin position="208"/>
            <end position="412"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF19289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF19290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEF96418.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="416" mass="45904" checksum="C95F605E4488C84B" modified="2011-07-27" version="1">MDIVEKIINIGEKEGFEVEVFISKGISSSIDLDGESVDSVETSKDFGVGVRVIKDGKVGFAYSTKEDVNVVYKAMKNLVEDKFVSFAEKEKFKEPKGIFYKDILKLDEEEMLSDLILMKDIAKDEGVTVTGGGISKSYEYNRIVNSNGVDVEEEFTFYSAYLSVIYENETGYEGLTKTKRFDVENIAKKAVELAKKSVNGKTITYDGDIILSPRALSSLLPYTLIPSFSAENVQRNRSVLKDKLGEQVFGESITIVDDGTLDNANYSSKVDGEGCPTKRTVLVENGVLKSYLYDIKRANIDGKECTGNGMRDYSSLPYISPSNFIIEPVDKLENFDNYVYVNGVIGSHTSNPITGDFAVEIQNAFMVKGGEEIPIKKGLLSGNIFECLKDAVPLNDVEQRGKLISPSIVIKGHIVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>F6BD65</accession>
    <name>F6BD65_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glutamine amidotransferase of anthranilate synthase</fullName>
            <ecNumber evidence="2">4.1.3.27</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0883</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EC" id="4.1.3.27" evidence="2"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96426.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799028.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0883"/>
    <dbReference type="MEROPS" id="C26.959"/>
    <dbReference type="EnsemblBacteria" id="AEF96426">
        <property type="protein sequence ID" value="AEF96426"/>
        <property type="gene ID" value="Metig_0883"/>
    </dbReference>
    <dbReference type="GeneID" id="10643727"/>
    <dbReference type="KEGG" id="mig:Metig_0883"/>
    <dbReference type="HOGENOM" id="CLU_014340_1_2_2"/>
    <dbReference type="OMA" id="HQVVIYR"/>
    <dbReference type="OrthoDB" id="100703at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004049">
        <property type="term" value="F:anthranilate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006541">
        <property type="term" value="P:glutamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01743">
        <property type="entry name" value="GATase1_Anthranilate_Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017926">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006221">
        <property type="entry name" value="TrpG/PapA_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00117">
        <property type="entry name" value="GATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="SSF52317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00566">
        <property type="entry name" value="trpG_papA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="2" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GATase" evidence="1">
        <location>
            <begin position="5"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96426.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="193" mass="21691" checksum="242C6B6B4A24A290" modified="2011-07-27" version="1">MKVLIIDNIDSFVWNLVQYVGMLKHKVKLVDNTITLSEIRKINPDRIIISPGPKTPKEAGNCIKIIQEIDDIPILGVCLGHQCIVEAFGGEVGRAKHIMHGKTSKIEHDGEGIYKDIPNPFYGTRYHSLVAYEVPKELKITAKSLDDGYVMGVRHKKLPIEGVQYHPESVLTHSEEMPFPKLGLKLIKNFIEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-06-02" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>F6BD66</accession>
    <name>F6BD66_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_0884</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96427.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799029.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96427">
        <property type="protein sequence ID" value="AEF96427"/>
        <property type="gene ID" value="Metig_0884"/>
    </dbReference>
    <dbReference type="GeneID" id="10643728"/>
    <dbReference type="KEGG" id="mig:Metig_0884"/>
    <dbReference type="HOGENOM" id="CLU_1318559_0_0_2"/>
    <dbReference type="OMA" id="CECAILV"/>
    <dbReference type="OrthoDB" id="87261at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF96427.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="209" mass="25092" checksum="5FCDD3675E395407" modified="2011-07-27" version="1">METLKNEIRKIFRNDLIHKPERVEIEEKDTYIIVKVKENATEADFKELKIEFPKEIEILPLLIDKDKYYTVFNINGNKKCECAILVKENDSYYLTLVEMKSTLRTFRIKKLIEIKEKFKCSLYVSLIILKLFDIIPKKIYGLIPHLRKETISPTTFDIANMDRDSSEREFLKEWESSELKFEWIYNTKIVLHKREFNEDSINWAEINTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>F6BD72</accession>
    <name>F6BD72_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="3">FeoA family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_0890</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96433.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799035.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0890"/>
    <dbReference type="EnsemblBacteria" id="AEF96433">
        <property type="protein sequence ID" value="AEF96433"/>
        <property type="gene ID" value="Metig_0890"/>
    </dbReference>
    <dbReference type="GeneID" id="10643734"/>
    <dbReference type="KEGG" id="mig:Metig_0890"/>
    <dbReference type="HOGENOM" id="CLU_150646_6_3_2"/>
    <dbReference type="OMA" id="AMKIMVE"/>
    <dbReference type="OrthoDB" id="114771at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046914">
        <property type="term" value="F:transition metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007167">
        <property type="entry name" value="Fe-transptr_FeoA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038157">
        <property type="entry name" value="FeoA_core_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008988">
        <property type="entry name" value="Transcriptional_repressor_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04023">
        <property type="entry name" value="FeoA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00899">
        <property type="entry name" value="FeoA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50037">
        <property type="entry name" value="SSF50037"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FeoA" evidence="2">
        <location>
            <begin position="2"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF96433.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="74" mass="7884" checksum="694A77640EF25BA9" modified="2011-07-27" version="1">MMSLADVEEGKVVVIKDILAGYGLKNRLQSMGLVIGDKVKVLKNSHGPVIVENRGVKIALGRGVAMKIIVGDIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>F6BD93</accession>
    <name>F6BD93_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 5">Protein translation factor SUI1 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Metig_0911</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4 5">Belongs to the SUI1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96454.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799056.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0911"/>
    <dbReference type="EnsemblBacteria" id="AEF96454">
        <property type="protein sequence ID" value="AEF96454"/>
        <property type="gene ID" value="Metig_0911"/>
    </dbReference>
    <dbReference type="GeneID" id="10643755"/>
    <dbReference type="KEGG" id="mig:Metig_0911"/>
    <dbReference type="HOGENOM" id="CLU_082805_6_1_2"/>
    <dbReference type="OMA" id="TIRDSWI"/>
    <dbReference type="OrthoDB" id="118816at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd11567">
        <property type="entry name" value="YciH_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00604">
        <property type="entry name" value="SUI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001950">
        <property type="entry name" value="SUI1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022851">
        <property type="entry name" value="SUI1_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005872">
        <property type="entry name" value="SUI1_arc_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036877">
        <property type="entry name" value="SUI1_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01253">
        <property type="entry name" value="SUI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037511">
        <property type="entry name" value="Transl_init_SUI1_pro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55159">
        <property type="entry name" value="SSF55159"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01158">
        <property type="entry name" value="SUI1_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50296">
        <property type="entry name" value="SUI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4 5" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4 5" id="KW-0810">Translation regulation</keyword>
    <feature type="domain" description="SUI1" evidence="6">
        <location>
            <begin position="27"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR037511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEF96454.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="102" mass="11558" checksum="35199EC012ED39B6" modified="2011-07-27" version="1">MPEICPVCGLPKDLCVCEEIAKEEQKIKVYVTKRRFGKLMTIIEGFDTDLIDIKELAKKLKDICACGGTVKDNAIELQGDHRKKVEEILVKMGFSKDMIDVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>F6BD98</accession>
    <name>F6BD98_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">HVO_2753_ZBP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0916</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96459.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799061.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0916"/>
    <dbReference type="EnsemblBacteria" id="AEF96459">
        <property type="protein sequence ID" value="AEF96459"/>
        <property type="gene ID" value="Metig_0916"/>
    </dbReference>
    <dbReference type="GeneID" id="10643760"/>
    <dbReference type="KEGG" id="mig:Metig_0916"/>
    <dbReference type="HOGENOM" id="CLU_196471_1_0_2"/>
    <dbReference type="OMA" id="KCGFEGP"/>
    <dbReference type="OrthoDB" id="123853at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044720">
        <property type="entry name" value="HVO_2753-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011668">
        <property type="entry name" value="HVO_2753-like_ZBP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40733">
        <property type="entry name" value="PTHR40733"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07754">
        <property type="entry name" value="HVO_2753_ZBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HVO_2753_ZBP" evidence="1">
        <location>
            <begin position="5"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96459.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="54" mass="6153" checksum="2BE8CB5EA4A4F156" modified="2011-07-27" version="1">MKYVCISCNAEITPREHATRFLCPNCGKVEIVRCEKCRKLSNPYKCPECGFEGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDI1</accession>
    <name>F6BDI1_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">AAA_31 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1000</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96542.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799144.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1000"/>
    <dbReference type="EnsemblBacteria" id="AEF96542">
        <property type="protein sequence ID" value="AEF96542"/>
        <property type="gene ID" value="Metig_1000"/>
    </dbReference>
    <dbReference type="GeneID" id="10643847"/>
    <dbReference type="KEGG" id="mig:Metig_1000"/>
    <dbReference type="HOGENOM" id="CLU_037612_0_3_2"/>
    <dbReference type="OMA" id="DIDCIGV"/>
    <dbReference type="OrthoDB" id="72034at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025669">
        <property type="entry name" value="AAA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13614">
        <property type="entry name" value="AAA_31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA_31" evidence="1">
        <location>
            <begin position="5"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96542.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="253" mass="28244" checksum="61B23813C1FE56BF" modified="2011-07-27" version="1">MRLGFYNLQGGTGKTTVAINLAYYLSEKAKTIYIDCDLYAGTSGILFGYENDPHNLNSYLSGNSSLDEVIHTYNNLAIIPSDVSPDAFNADMDFERFAEMVDILEETYDIIIFDLPPNITEGNLLFSSIDKKLINKMIIVGEDSIPGIVNTLKTQELLTAIGIGVTGTIINKYRGIVDFEDILDNVIALLPYDKKVEEQWALNKPVVEIKSKFGRELIYLAEDLAHVYIEKDLAAMRALKLAKELMGLAEDEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDN6</accession>
    <name>F6BDN6_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Biotin/acetyl-CoA-carboxylase ligase</fullName>
            <ecNumber evidence="2">6.3.4.15</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1058</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EC" id="6.3.4.15" evidence="2"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96597.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1058"/>
    <dbReference type="EnsemblBacteria" id="AEF96597">
        <property type="protein sequence ID" value="AEF96597"/>
        <property type="gene ID" value="Metig_1058"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_1058"/>
    <dbReference type="HOGENOM" id="CLU_051096_3_1_2"/>
    <dbReference type="OMA" id="VGDCIHL"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004077">
        <property type="term" value="F:biotin-[acetyl-CoA-carboxylase] ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd16442">
        <property type="entry name" value="BPL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004408">
        <property type="entry name" value="Biotin_CoA_COase_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004143">
        <property type="entry name" value="BPL_LPL_catalytic"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03099">
        <property type="entry name" value="BPL_LplA_LipB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="SSF55681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00121">
        <property type="entry name" value="birA_ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51733">
        <property type="entry name" value="BPL_LPL_CATALYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BPL/LPL catalytic" evidence="1">
        <location>
            <begin position="1"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96597.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="242" mass="27817" checksum="6AF025E189F3B26B" modified="2011-07-27" version="1">MGRFEIIHLNRIDSTNTYAYKLAKEGKRDIVVVADEQTSGRGRLNRVWFSDFGGLYFSIVLESDVDKFPVLNFLAPICMVKTLKNYSNKNYGIKWPNDIIVNNKKICGILSEINVEYGFMVLGIGINVNNKIREEIRDIATSLKEVEGKEFDKMEILKTFLEIFESYLNNLSPENILKEYKENSLTLNEYVKIITPNREIFGHVVDITYEGIVLQTDEKLEIVDVGDCIHLRKVFPSPYGSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDP0</accession>
    <name>F6BDP0_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Formate-dependent phosphoribosylglycinamide formyltransferase</fullName>
            <ecNumber evidence="3">6.3.1.21</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">5'-phosphoribosylglycinamide transformylase 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="3">Formate-dependent GAR transformylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="3">GAR transformylase 2</fullName>
            <shortName evidence="3">GART 2</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="3">Non-folate glycinamide ribonucleotide transformylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="3">Phosphoribosylglycinamide formyltransferase 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">purT</name>
        <name evidence="6" type="ordered locus">Metig_1062</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Involved in the de novo purine biosynthesis. Catalyzes the transfer of formate to 5-phospho-ribosyl-glycinamide (GAR), producing 5-phospho-ribosyl-N-formylglycinamide (FGAR). Formate is provided by PurU via hydrolysis of 10-formyl-tetrahydrofolate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>ATP + formate + N(1)-(5-phospho-beta-D-ribosyl)glycinamide = ADP + H(+) + N(2)-formyl-N(1)-(5-phospho-beta-D-ribosyl)glycinamide + phosphate</text>
            <dbReference type="Rhea" id="RHEA:24829"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:143788"/>
            <dbReference type="ChEBI" id="CHEBI:147286"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.1.21"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Purine metabolism; IMP biosynthesis via de novo pathway; N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide from N(1)-(5-phospho-D-ribosyl)glycinamide (formate route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the PurK/PurT family.</text>
    </comment>
    <dbReference type="EC" id="6.3.1.21" evidence="3"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96601.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799202.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1062"/>
    <dbReference type="EnsemblBacteria" id="AEF96601">
        <property type="protein sequence ID" value="AEF96601"/>
        <property type="gene ID" value="Metig_1062"/>
    </dbReference>
    <dbReference type="GeneID" id="10643916"/>
    <dbReference type="KEGG" id="mig:Metig_1062"/>
    <dbReference type="HOGENOM" id="CLU_011534_1_3_2"/>
    <dbReference type="OMA" id="GMVTMIT"/>
    <dbReference type="OrthoDB" id="57699at2157"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00127"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043815">
        <property type="term" value="F:phosphoribosylglycinamide formyltransferase 2 activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004644">
        <property type="term" value="F:phosphoribosylglycinamide formyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01643">
        <property type="entry name" value="PurT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003135">
        <property type="entry name" value="ATP-grasp_carboxylate-amine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005862">
        <property type="entry name" value="PurT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011054">
        <property type="entry name" value="Rudment_hybrid_motif"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02222">
        <property type="entry name" value="ATP-grasp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51246">
        <property type="entry name" value="SSF51246"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="SSF52440"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01142">
        <property type="entry name" value="purT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="6" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="1 3" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="ATP-grasp" evidence="5">
        <location>
            <begin position="117"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="region of interest" description="5'-phosphoribosylglycinamide binding" evidence="3">
        <location>
            <begin position="20"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="5'-phosphoribosylglycinamide binding" evidence="3">
        <location>
            <begin position="359"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="80"/>
        </location>
        <ligand>
            <name>N(1)-(5-phospho-beta-D-ribosyl)glycinamide</name>
            <dbReference type="ChEBI" id="CHEBI:143788"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="112"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="153"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="158"/>
            <end position="163"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="193"/>
            <end position="196"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="201"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="265"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="277"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="284"/>
        </location>
        <ligand>
            <name>N(1)-(5-phospho-beta-D-ribosyl)glycinamide</name>
            <dbReference type="ChEBI" id="CHEBI:143788"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="352"/>
        </location>
        <ligand>
            <name>N(1)-(5-phospho-beta-D-ribosyl)glycinamide</name>
            <dbReference type="ChEBI" id="CHEBI:143788"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEF96601.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="388" mass="42942" checksum="8EAE920BB38F1286" modified="2011-07-27" version="1">MIGTPLLDGATKILLLGSGELGKEVVIEAQRLGVECIAVDRYQNAPAMQVAHRSYVIDMKDRDALRAIIERENPDYIVPEIEAINTDTLIEMEKEGYTVIPTAKATKITMDREGIRRLAAEELKLKTAKYEFATSLDELKEAVEKIGIPCVVKPIMSSSGKGQSVIKSKEDIEKAWEIAQKGARGLANKVIVEEFINFDYEITLLTARTVEGTKFCEPIGHIQIDGDYHESWQPHPMSEELKKDAQDIAKKITDALGGYGIFGVELFVRGDEVIFSEVSPRPHDTGMVTMVTQEMSEFEIHVRAILGLPVSTKLISCGASHVIKSDIHKWNPKYDISEALKVPNTKIRLFGKPVATVGRRMGVALATADSIEEARKNAEKCAHAVKIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-06-02" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDP5</accession>
    <name>F6BDP5_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1067</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96606.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96606">
        <property type="protein sequence ID" value="AEF96606"/>
        <property type="gene ID" value="Metig_1067"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_1067"/>
    <dbReference type="HOGENOM" id="CLU_2299401_0_0_2"/>
    <dbReference type="OMA" id="GNDMINH"/>
    <dbReference type="OrthoDB" id="131582at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="51"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="81"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96606.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="105" mass="10728" checksum="E52D91610802ADCD" modified="2011-07-27" version="1">MKFLKKEVYKIFGTLVLVSMIGALVAEPVALGDAGLVYYYYYKNKDTGNDMINHGAATAGVGSILFGVGTYALENAAAGSILYYAGAGLAATGVGLVIVGVGLLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDR3</accession>
    <name>F6BDR3_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Acetyl-CoA decarbonylase/synthase complex subunit delta</fullName>
            <shortName evidence="1">ACDS complex subunit delta</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Corrinoid/iron-sulfur component small subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">cdhD</name>
        <name evidence="3" type="ordered locus">Metig_1085</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Part of a complex that catalyzes the reversible cleavage of acetyl-CoA, allowing autotrophic growth from CO(2). Probably maintains the overall quaternary structure of the ACDS complex.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterodimer of delta and gamma chains. The ACDS complex is made up of alpha, epsilon, beta, gamma and delta chains with a probable stoichiometry of (alpha(2)epsilon(2))(4)-beta(8)-(gamma(1)delta(1))(8).</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the CdhD family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96624.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799225.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1085"/>
    <dbReference type="EnsemblBacteria" id="AEF96624">
        <property type="protein sequence ID" value="AEF96624"/>
        <property type="gene ID" value="Metig_1085"/>
    </dbReference>
    <dbReference type="GeneID" id="10643939"/>
    <dbReference type="KEGG" id="mig:Metig_1085"/>
    <dbReference type="HOGENOM" id="CLU_040403_0_0_2"/>
    <dbReference type="OMA" id="VMDPTTC"/>
    <dbReference type="OrthoDB" id="72026at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01135">
        <property type="entry name" value="CdhD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016041">
        <property type="entry name" value="Ac-CoA_synth_d_su_TIM-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004486">
        <property type="entry name" value="CO_DH/Ac-CoA_synth_dsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011005">
        <property type="entry name" value="Dihydropteroate_synth-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03599">
        <property type="entry name" value="CdhD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51717">
        <property type="entry name" value="SSF51717"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00381">
        <property type="entry name" value="cdhD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CdhD" evidence="2">
        <location>
            <begin position="76"/>
            <end position="323"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF96624.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="392" mass="43774" checksum="6CBAFDB384AF5FFC" modified="2011-07-27" version="1">MDLNAIIKIIEKVGRIEIENIEITADELIINIPSAPPVVIPQTPTIKEKLAEEGIIEVPEVDWEKPVETYKGYIREVQFGKSKSEGGRGKVIKIGGQRTLYRFEEPQPNPPVVTFDIFDMPMHGLPAPVKKYFADVMEDPCEWARKCVKEFGADMVTIHHISTDPKIKDTPPREAAKLMEELLQAVDVPFVIGGSGDPKKDPLVLEACAEAAEGERCLLASANLELDYKKVAEAAMKYDHNVLSWTIMDPNMAKDLNRRLISVGLDPNRIVMDPTTCALGYGIEFSINAMTRLRLAGLKGDELVNMPMSSGTTNAIGAREAWMKNPEWGDREYRLPLWEITTGLTMLMSGVDIFMMLHPLSIKVLKEIGKSLITKPGEVKINTNNYEWITAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDT1</accession>
    <name>F6BDT1_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_1103</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96642.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1103"/>
    <dbReference type="EnsemblBacteria" id="AEF96642">
        <property type="protein sequence ID" value="AEF96642"/>
        <property type="gene ID" value="Metig_1103"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_1103"/>
    <dbReference type="HOGENOM" id="CLU_2581363_0_0_2"/>
    <dbReference type="OMA" id="MHYSIIK"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF96642.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="96" mass="11344" checksum="F340EBB70F896D2A" modified="2011-07-27" version="1">MNNKEVFGEFDVVEDDIMHYSIIKPKYKKEEITIDKGSLKTKRKFAFLLEVGDKLLNSREFYANDDVEVVVDYSFSDSKRPKEKIILYIIEDLTKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2020-06-17" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDU4</accession>
    <name>F6BDU4_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1116</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96655.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96655">
        <property type="protein sequence ID" value="AEF96655"/>
        <property type="gene ID" value="Metig_1116"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_1116"/>
    <dbReference type="HOGENOM" id="CLU_3178572_0_0_2"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="24"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96655.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="46" mass="5530" checksum="6D77C01E8E44945E" modified="2011-07-27" version="1">MTKQSFATKIFPNDNYPLINVRDYIDYILFIVVCRIFCTYLTTIDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-06-02" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDU8</accession>
    <name>F6BDU8_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_1120</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96659.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799260.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96659">
        <property type="protein sequence ID" value="AEF96659"/>
        <property type="gene ID" value="Metig_1120"/>
    </dbReference>
    <dbReference type="GeneID" id="10643978"/>
    <dbReference type="KEGG" id="mig:Metig_1120"/>
    <dbReference type="HOGENOM" id="CLU_2534703_0_0_2"/>
    <dbReference type="OMA" id="MTIGAEI"/>
    <dbReference type="OrthoDB" id="143160at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF96659.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="83" mass="7919" checksum="E6AD386CFEEE5D93" modified="2011-07-27" version="1">MLCMMGAVLAEPVVLGNVGYYVGKELAGEAGSVVGGYIGTKAGEYAGESAGIILAELYAEEGAAAGSIAGPVGAIIGGITGAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDV9</accession>
    <name>F6BDV9_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Cyclase family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_1131</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96670.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799271.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1131"/>
    <dbReference type="EnsemblBacteria" id="AEF96670">
        <property type="protein sequence ID" value="AEF96670"/>
        <property type="gene ID" value="Metig_1131"/>
    </dbReference>
    <dbReference type="GeneID" id="10643989"/>
    <dbReference type="KEGG" id="mig:Metig_1131"/>
    <dbReference type="HOGENOM" id="CLU_030671_3_2_2"/>
    <dbReference type="OMA" id="FKFFGFP"/>
    <dbReference type="OrthoDB" id="93116at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004061">
        <property type="term" value="F:arylformamidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019441">
        <property type="term" value="P:tryptophan catabolic process to kynurenine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007325">
        <property type="entry name" value="KFase/CYL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037175">
        <property type="entry name" value="KFase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31118">
        <property type="entry name" value="PTHR31118"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04199">
        <property type="entry name" value="Cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102198">
        <property type="entry name" value="SSF102198"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF96670.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="200" mass="22412" checksum="29E2B909E75BB3B9" modified="2011-07-27" version="1">MGDIKDLLKNKNTIIDLSHEVMDFVYPGDPKFCISTLKFENFMVSEIKMGSHISTHVDYPKHVGLDNDSVVDVIVGEGFCVNIGNLEEFLSKSNYIRNIDVLLIYTSISSLWGREEYFERGINIKDYLNDLLSLGIKAIGVDCASIGDYDVHKSLLLNGILIIENLTNLDILVNKSFFFIGLPLKIKNIDGVPIRAIAIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDW5</accession>
    <name>F6BDW5_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Metal dependent phosphohydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1137</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96676.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799277.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1137"/>
    <dbReference type="EnsemblBacteria" id="AEF96676">
        <property type="protein sequence ID" value="AEF96676"/>
        <property type="gene ID" value="Metig_1137"/>
    </dbReference>
    <dbReference type="GeneID" id="10643995"/>
    <dbReference type="KEGG" id="mig:Metig_1137"/>
    <dbReference type="HOGENOM" id="CLU_073842_1_0_2"/>
    <dbReference type="OMA" id="GRSKTHG"/>
    <dbReference type="OrthoDB" id="88300at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004454">
        <property type="entry name" value="HD-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006674">
        <property type="entry name" value="HD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006675">
        <property type="entry name" value="HDIG_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01966">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00471">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00277">
        <property type="entry name" value="HDIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00295">
        <property type="entry name" value="TIGR00295"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51831">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HD" evidence="1">
        <location>
            <begin position="15"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96676.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="166" mass="18404" checksum="9BA660AF4D44CE7B" modified="2011-07-27" version="1">MDALSLLKELCDKNVVEHCIAVSNYAYEIALQIKNNGHPVDLELVRLGGLLHDIGRSKTHGIEHGIAGAEILRMHGFDERLALIAERHIGAGITKKEAIELGLPPKDYVPVTLEEKIVAHADNLVSGTKRVDIGFVVDKFRRKLGDNHPAIERILRLNDEINSLLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDZ7</accession>
    <name>F6BDZ7_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Radical SAM domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1170</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96708.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799308.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1170"/>
    <dbReference type="EnsemblBacteria" id="AEF96708">
        <property type="protein sequence ID" value="AEF96708"/>
        <property type="gene ID" value="Metig_1170"/>
    </dbReference>
    <dbReference type="GeneID" id="10644030"/>
    <dbReference type="KEGG" id="mig:Metig_1170"/>
    <dbReference type="HOGENOM" id="CLU_533842_0_0_2"/>
    <dbReference type="OMA" id="HTPGKYL"/>
    <dbReference type="OrthoDB" id="19362at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.30.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaB/NifB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023404">
        <property type="entry name" value="rSAM_horseshoe"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Radical SAM core" evidence="1">
        <location>
            <begin position="178"/>
            <end position="440"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96708.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="509" mass="58156" checksum="DD61A47E977B4933" modified="2011-07-27" version="1">MRFLILDGYTDEPAGLGVPPYIGIYPRYIAGVLDKFNQEVHYITIDKLREIRGTFDFNKFDAIIAICGFHTPGKYLTANPATLREIVSLLYNFKGLKILAGPAATKFGSSIEGGTIKDESKLKAFFDIIIEGDAEAVLHDYFLNNCKIENIDTKRLRTYDELREFAIRGAKIVKQHPSYPYIIAEIETYRGCARALSGGCSFCTEPRRFGTPNYRDAKDIIEEIKALYSYGIKYFRIGRQPCIFSYKSKESEKEEVPKPNVEEIEKLFKGIWNVVNPEVLHIDNANPAVIARHEEESREVAKILVRYCTGGNVAAFGVESFDEKVIKKNNLLTTPEDVLKAVEILNEIGGKRSETGLPYLLPGINLLYGLRGETKETFRINYEYLKEIYDRGLMLRRINIRQVVPFFGTELTIKDVEKAKKRKSLFLSFKEKVRKEIDNPMLKRVVPKGTILKNVFVEVKERDDLFFGRQFGSYPILVGIKEKNLKIGEFVDVEVIDYGRRSITAKKLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-06-02" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>F6BE20</accession>
    <name>F6BE20_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">FlpE-related protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_1193</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96731.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799330.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96731">
        <property type="protein sequence ID" value="AEF96731"/>
        <property type="gene ID" value="Metig_1193"/>
    </dbReference>
    <dbReference type="GeneID" id="10644053"/>
    <dbReference type="KEGG" id="mig:Metig_1193"/>
    <dbReference type="HOGENOM" id="CLU_2079438_0_0_2"/>
    <dbReference type="OMA" id="RPKENPK"/>
    <dbReference type="OrthoDB" id="116306at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF96731.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="118" mass="13475" checksum="56CE09537E0D6BE4" modified="2011-07-27" version="1">MNIKISIEKLKLVRPKENPKNANLKLDVNWDITYKKISNNSFGYICNLKMEGEFPTLFVIEGAVEFEESINHIPDDISQSILDSLFQILVNMITITKEIHIEVKDFPLAEIPAQKVAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>F6BE42</accession>
    <name>F6BE42_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="3">NADH dehydrogenase (Quinone)</fullName>
            <ecNumber evidence="3">1.6.99.5</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_0016</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.6.99.5" evidence="3"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95578.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798188.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0016"/>
    <dbReference type="EnsemblBacteria" id="AEF95578">
        <property type="protein sequence ID" value="AEF95578"/>
        <property type="gene ID" value="Metig_0016"/>
    </dbReference>
    <dbReference type="GeneID" id="10642851"/>
    <dbReference type="KEGG" id="mig:Metig_0016"/>
    <dbReference type="HOGENOM" id="CLU_015134_1_2_2"/>
    <dbReference type="OMA" id="CDPCFTC"/>
    <dbReference type="OrthoDB" id="4328at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008901">
        <property type="term" value="F:ferredoxin hydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016151">
        <property type="term" value="F:nickel cation binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016651">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048038">
        <property type="term" value="F:quinone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.645.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001135">
        <property type="entry name" value="NADH_Q_OxRdtase_suD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001501">
        <property type="entry name" value="Ni-dep_hyd_lsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018194">
        <property type="entry name" value="Ni-dep_hyd_lsu_Ni_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029014">
        <property type="entry name" value="NiFe-Hase_large"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00346">
        <property type="entry name" value="Complex1_49kDa"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00374">
        <property type="entry name" value="NiFeSe_Hases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56762">
        <property type="entry name" value="SSF56762"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00507">
        <property type="entry name" value="NI_HGENASE_L_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Complex1_49kDa" evidence="2">
        <location>
            <begin position="118"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="domain" description="Complex1_49kDa" evidence="2">
        <location>
            <begin position="300"/>
            <end position="364"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF95578.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="373" mass="42915" checksum="CB0952F315943369" modified="2011-07-27" version="1">MYEGEIAIGPVHSTMLEPHRLRLFIEDEVVKDAELTIGVNHRGVERLMEGLPVEKACILCEKICGICSHIHLWSAVRVVERGCNIEVPERANYIRVIVEELERLHSHTLLFGHAFEILGHETMAMRAFMLREPIMQTFFDISGSRVHYSCPIIGGIRPRCNIKDSQIPTILERLEKFEEKLKKLMERTLNDPMLVSRMKDIGVLDRKTAAKYHAVGPTARGSGIKSDMRKMGYVPEYEPFEFEEILFDDGDVLSRIAVRFYECFESIKILKQALKALPQLPEKIYNGDYELYEFKPIDCYTEAQRGQQYYSYGLDSEGRVRHAKVRTPTATNLAAMEEILKDYHVTDAELIIASCDPCFTCTDRLIITKEYIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-06-02" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>F6BE66</accession>
    <name>F6BE66_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1205</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96743.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96743">
        <property type="protein sequence ID" value="AEF96743"/>
        <property type="gene ID" value="Metig_1205"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_1205"/>
    <dbReference type="HOGENOM" id="CLU_2766085_0_0_2"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="51"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96743.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="69" mass="8187" checksum="12411E36865A75D7" modified="2011-07-27" version="1">MDALLFISLSFFINMVIYSVSKNRTNKKFKNVVGKIKMNHLYLMYGKKAKYDKILLPILIGIFIILYFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>F6BE87</accession>
    <name>F6BE87_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">NADPH-dependent F420 reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1227</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96764.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799363.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1227"/>
    <dbReference type="EnsemblBacteria" id="AEF96764">
        <property type="protein sequence ID" value="AEF96764"/>
        <property type="gene ID" value="Metig_1227"/>
    </dbReference>
    <dbReference type="GeneID" id="10644088"/>
    <dbReference type="KEGG" id="mig:Metig_1227"/>
    <dbReference type="HOGENOM" id="CLU_076368_1_0_2"/>
    <dbReference type="OMA" id="IDTDVMV"/>
    <dbReference type="OrthoDB" id="83005at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070967">
        <property type="term" value="F:coenzyme F420 binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016651">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006740">
        <property type="term" value="P:NADPH regeneration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010185">
        <property type="entry name" value="NpdG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028939">
        <property type="entry name" value="P5C_Rdtase_cat_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03807">
        <property type="entry name" value="F420_oxidored"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01915">
        <property type="entry name" value="npdG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F420_oxidored" evidence="1">
        <location>
            <begin position="2"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96764.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="221" mass="24107" checksum="1D1FB76890D47D16" modified="2011-07-27" version="1">MKIAILGGTGEQGFGLALRFAKNHEVIIGSRKKERAEDAAEKVKSILKERGIDANVRGLENKDAAKEGDIVILSLPYEYTLSTIKDLKEHLDGKIVVSIGVPLATAIGDKPTRPLPPPQGSVAEMVQEKLKNSKVVSAFQNVCCRALEDLDNPVDCDILVCGDDEEAKKVVMELAEEIEGVRAIDCGELEMSRIIEQITPLLIKLNIKYKLKGTGIRITGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEA5</accession>
    <name>F6BEA5_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Tetrahydromethanopterin S-methyltransferase subunit H</fullName>
            <ecNumber evidence="3">2.1.1.86</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">N5-methyltetrahydromethanopterin--coenzyme M methyltransferase subunit H</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">mtrH</name>
        <name evidence="4" type="ordered locus">Metig_1245</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Part of a complex that catalyzes the formation of methyl-coenzyme M and tetrahydromethanopterin from coenzyme M and methyl-tetrahydromethanopterin. This is an energy-conserving, sodium-ion translocating step. MtrH catalyzes the transfer of the methyl group from methyl-tetrahydromethanopterin to the corrinoid prosthetic group of MtrA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>5-methyl-5,6,7,8-tetrahydromethanopterin + coenzyme M + 2 Na(+)(in) = 5,6,7,8-tetrahydromethanopterin + methyl-coenzyme M + 2 Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:53492"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:58103"/>
            <dbReference type="ChEBI" id="CHEBI:58116"/>
            <dbReference type="ChEBI" id="CHEBI:58286"/>
            <dbReference type="ChEBI" id="CHEBI:58319"/>
            <dbReference type="EC" id="2.1.1.86"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">One-carbon metabolism; methanogenesis from CO(2); methyl-coenzyme M from 5,10-methylene-5,6,7,8-tetrahydromethanopterin: step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">The complex is composed of 8 subunits; MtrA, MtrB, MtrC, MtrD, MtrE, MtrF, MtrG and MtrH.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the MtrH family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.86" evidence="3"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96782.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799381.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1245"/>
    <dbReference type="EnsemblBacteria" id="AEF96782">
        <property type="protein sequence ID" value="AEF96782"/>
        <property type="gene ID" value="Metig_1245"/>
    </dbReference>
    <dbReference type="GeneID" id="10644108"/>
    <dbReference type="KEGG" id="mig:Metig_1245"/>
    <dbReference type="HOGENOM" id="CLU_048697_0_0_2"/>
    <dbReference type="OMA" id="YARHKIV"/>
    <dbReference type="OrthoDB" id="86442at2157"/>
    <dbReference type="UniPathway" id="UPA00640">
        <property type="reaction ID" value="UER00698"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030269">
        <property type="term" value="F:tetrahydromethanopterin S-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019386">
        <property type="term" value="P:methanogenesis, from carbon dioxide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01501">
        <property type="entry name" value="MtrH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011005">
        <property type="entry name" value="Dihydropteroate_synth-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023467">
        <property type="entry name" value="MeTrfase_MtrH/MtxH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028342">
        <property type="entry name" value="MtrH"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02007">
        <property type="entry name" value="MtrH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF500206">
        <property type="entry name" value="MtrH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004960">
        <property type="entry name" value="MtrH_MtxH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51717">
        <property type="entry name" value="SSF51717"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01114">
        <property type="entry name" value="mtrH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0484">Methanogenesis</keyword>
    <keyword evidence="3 4" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="2 3" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEF96782.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="319" mass="34471" checksum="E24DEB7B3A54AC15" modified="2011-07-27" version="1">MFKFEKEQMVVEIAGRKIGGQPGEYPTALAGTIFYARHKIVEDPKKGIFDKQAAEDLINKQAEMEDITGNPALVQVFGGTPEALVNYIDFVAEVWDGPMLLDSTSGEARMAAARRATEAGYANQCIYNSINVSIEDEEFQTLVESDLEASIVLCFDPMDPSVEGKINVLTNGGKTKDVGMLELAEKAGIKYPLIDVAVTPLGNGAGNAVRASFAVKAKFGLPVGSGIHNIPSAWDWLREFRKKLREEGLTQLAKDVHHVCDIGANLVQVMASGDFVLYGPIDNAQLAFPAVAMTDAVIAEAAKDMGINPIDTHPFNKLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEB2</accession>
    <name>F6BEB2_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">CRISPR-associated protein DxTHG motif protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_1252</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96789.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799388.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1252"/>
    <dbReference type="EnsemblBacteria" id="AEF96789">
        <property type="protein sequence ID" value="AEF96789"/>
        <property type="gene ID" value="Metig_1252"/>
    </dbReference>
    <dbReference type="GeneID" id="10644115"/>
    <dbReference type="KEGG" id="mig:Metig_1252"/>
    <dbReference type="HOGENOM" id="CLU_025124_0_0_2"/>
    <dbReference type="OMA" id="FDITHAF"/>
    <dbReference type="OrthoDB" id="20895at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013383">
        <property type="entry name" value="CRISPR-assoc_prot_DxTHG_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011742">
        <property type="entry name" value="CRISPR-assoc_prot_TM1812"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019016">
        <property type="entry name" value="Csx1-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09455">
        <property type="entry name" value="Cas_DxTHG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02221">
        <property type="entry name" value="cas_TM1812"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02549">
        <property type="entry name" value="CRISPR_DxTHG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF96789.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="420" mass="49898" checksum="1E8E7BF5DA8DAC6A" modified="2011-07-27" version="1">MPKKLISFLGAGKYEPCIYKYENKSSTESRFIQKALIELICKDWEEKDEIIILLTEKSEKMNWEGETYKGLKYELEEMDIKPEIKTIKIPDGNTEEEVWEIFNKIFEIINERDEIYIDITHSFRYLPMLATIILDYAKTLKGIKVKKIFYGNVFGEPRIMELQELDRLLDWNKAIDKFLTTGYVEDLYNLSKREYGKLHKENRELHELLKNNTGKLKKYMEYLATVRGDEINNFDYDEFKNEIDEIIKIIKKDSSLAPLIPLIEKIKDKFKDFKNNDKYNCLRAVSLCIEFNLIQQAYTLLQESVITLICKDLDLDYLDKEYRGLVSSALNIVARDLPKNEWKIPGKDEDEKKKNLELLKKIVDHLKTNNLTKLSKLYDKLTQYRNNINHAGFNKDKMEPKKFKNMINEIYRNILNFYSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2020-08-12" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>F6BED0</accession>
    <name>F6BED0_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1270</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96807.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96807">
        <property type="protein sequence ID" value="AEF96807"/>
        <property type="gene ID" value="Metig_1270"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_1270"/>
    <dbReference type="HOGENOM" id="CLU_211860_0_0_2"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="35"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96807.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="53" mass="5935" checksum="284FC84C8B6CB6EA" modified="2011-07-27" version="1">METRLNNINNLNHEVAEDIEQITTDLKRPFRGSKLVEILGYVVGLIVIGSVLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>F6BED2</accession>
    <name>F6BED2_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">DsrE family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_1272</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96809.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799407.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1272"/>
    <dbReference type="EnsemblBacteria" id="AEF96809">
        <property type="protein sequence ID" value="AEF96809"/>
        <property type="gene ID" value="Metig_1272"/>
    </dbReference>
    <dbReference type="GeneID" id="10644135"/>
    <dbReference type="KEGG" id="mig:Metig_1272"/>
    <dbReference type="HOGENOM" id="CLU_151801_2_0_2"/>
    <dbReference type="OMA" id="VKACGPC"/>
    <dbReference type="OrthoDB" id="91267at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027396">
        <property type="entry name" value="DsrEFH-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003787">
        <property type="entry name" value="Sulphur_relay_DsrE/F-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02635">
        <property type="entry name" value="DrsE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75169">
        <property type="entry name" value="SSF75169"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF96809.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="114" mass="12610" checksum="BA883762916D1215" modified="2011-07-27" version="1">MKFTVIITTAPYGKERAYSALRFALTSLLEGIEVNIFLIEDGVYVAKKNQNPAEVPNYLEFLKNCIEMGAKVKACGPCGRARGLSDEDLIEGVEFGTMHDLVAFVKESDKVITF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEE6</accession>
    <name>F6BEE6_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RPA_C domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_0046</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the replication factor A protein 2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95607.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798216.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF95607">
        <property type="protein sequence ID" value="AEF95607"/>
        <property type="gene ID" value="Metig_0046"/>
    </dbReference>
    <dbReference type="GeneID" id="10642881"/>
    <dbReference type="KEGG" id="mig:Metig_0046"/>
    <dbReference type="HOGENOM" id="CLU_1335077_0_0_2"/>
    <dbReference type="OMA" id="RYVAYKI"/>
    <dbReference type="OrthoDB" id="81848at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014892">
        <property type="entry name" value="RPA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08784">
        <property type="entry name" value="RPA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RPA_C" evidence="2">
        <location>
            <begin position="173"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08784"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF95607.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="229" mass="27186" checksum="082183676E3B2C0F" modified="2011-07-27" version="1">MRYVAYKIRPEEFLKNEIKNNALILEGHKIHRVRILGEVKNINIMKIISFEVDGVLVRYFGDKDLDIKEGDLVDVIGRVREHEGTKYIALEIVKKRNDDKEKWRELRNLEIEKTRKYLKYKEKDEKEEIEDELEDELFGTENVDETSEDKDVEEEILESIYGDTENEEVIKNEGIKDKILELIRENEGISLDEICDMLNMDEDEVYEIIQELIDEGEIYEPSPNKFKIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-06-02" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEF4</accession>
    <name>F6BEF4_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_0054</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95615.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798224.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF95615">
        <property type="protein sequence ID" value="AEF95615"/>
        <property type="gene ID" value="Metig_0054"/>
    </dbReference>
    <dbReference type="GeneID" id="10642889"/>
    <dbReference type="KEGG" id="mig:Metig_0054"/>
    <dbReference type="HOGENOM" id="CLU_1736435_0_0_2"/>
    <dbReference type="OMA" id="PKLYVVR"/>
    <dbReference type="OrthoDB" id="84856at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF95615.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="160" mass="18777" checksum="8E0C0EE67F51A860" modified="2011-07-27" version="1">MLRKIKRIFVGNKELPQQIPIKEEEYVIIGEDAPAYEIVGSEQIPLTEEKEKEEDREKIEEKVIEKVIKIPPKILTIRIKTPEDFINVREMIEHDIVIVNLEEIPVEGVIKDFLDFKKYLETLGYKLGRVGEYVILAVKDNVNIDRYSPQTIEINERDEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEI3</accession>
    <name>F6BEI3_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0083</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95644.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798253.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0083"/>
    <dbReference type="EnsemblBacteria" id="AEF95644">
        <property type="protein sequence ID" value="AEF95644"/>
        <property type="gene ID" value="Metig_0083"/>
    </dbReference>
    <dbReference type="GeneID" id="10642918"/>
    <dbReference type="KEGG" id="mig:Metig_0083"/>
    <dbReference type="HOGENOM" id="CLU_945320_0_0_2"/>
    <dbReference type="OMA" id="TIMPANI"/>
    <dbReference type="OrthoDB" id="74953at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005240">
        <property type="entry name" value="DUF389"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20992">
        <property type="entry name" value="PTHR20992"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04087">
        <property type="entry name" value="DUF389"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="113"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="135"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="171"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="203"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="230"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="257"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF95644.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="286" mass="30794" checksum="7676FF02CB8666AD" modified="2011-07-27" version="1">MRYMKIMIPKKFLNIVEEILKKNNAYSISIIEPLKSSIENGIIITCNAEAKDAEKIVLELKKLGLGEKGHGSITIMPANITFSCREEGLASTSLSALELYYKAKSMVKITKNVLIKVVLASIMGTIGIIEHNIPTLIGAMIIAPLVDTVMGSAIGTVLGDRELFIEGMKKELLCAGIVTVCAFVTGLFFVSKDLVLEYLSETSIALSSIVAIIAGISGGMSIASGKEYEIIGVTIDVSILIPALLMGMALATMDLNLIYTTSILLILNIVLLDIGGYIGLKYKMKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEJ5</accession>
    <name>F6BEJ5_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Pyridine nucleotide-disulfide oxidoreductase family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0095</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95656.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798265.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0095"/>
    <dbReference type="EnsemblBacteria" id="AEF95656">
        <property type="protein sequence ID" value="AEF95656"/>
        <property type="gene ID" value="Metig_0095"/>
    </dbReference>
    <dbReference type="GeneID" id="10642930"/>
    <dbReference type="KEGG" id="mig:Metig_0095"/>
    <dbReference type="HOGENOM" id="CLU_094970_1_1_2"/>
    <dbReference type="OMA" id="LQACQMT"/>
    <dbReference type="OrthoDB" id="100378at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032836">
        <property type="entry name" value="DsrE2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027396">
        <property type="entry name" value="DsrEFH-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13686">
        <property type="entry name" value="DrsE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75169">
        <property type="entry name" value="SSF75169"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF95656.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="138" mass="15279" checksum="57F41D463B667DC2" modified="2011-07-27" version="1">MTDKMALVVSEGTFDKAMMAFILGTIGATMGMEVHIFFTFFGLEILKKDAKPKLPGIYRLFGNFGVKMMEKKMKKVNIGSFEEMRQQAIDLGVNLYACSTSMSVMGIKKEDLIEGVEVVGATKFLDIAMDAKIQLFIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEK4</accession>
    <name>F6BEK4_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_0104</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95665.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0104"/>
    <dbReference type="EnsemblBacteria" id="AEF95665">
        <property type="protein sequence ID" value="AEF95665"/>
        <property type="gene ID" value="Metig_0104"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0104"/>
    <dbReference type="HOGENOM" id="CLU_803466_0_0_2"/>
    <dbReference type="OMA" id="LFEHHTR"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014846">
        <property type="entry name" value="DUF1786_pyruvate_format-lyase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08735">
        <property type="entry name" value="DUF1786"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF029129">
        <property type="entry name" value="DUF1786_pyruvate_format-lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="SSF53067"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF95665.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="351" mass="39567" checksum="2089AA726E9B41D0" modified="2011-07-27" version="1">MPYSIYIFSINHLGDKMEVLCIDIGKGTQDILYIDTNKNIENSIKLILPSPTVILAEKIKKMNEDLKIDGVIMGGGPVNKAISEHIKKGFKVEISENAARTIRDDLEEVKKKGIIIKESIENPNVIFRDLDFDMLKNIFTSIGKEFNPDFVCVACQDHGFVKGQSDRITRFKYFENKLKETKDPYKFYFEEKTDYFSRFDAILETLKENNYKGFVMDSKIASICGILNYAKENGIDEFIGLDIGNGHTLGVSIKDGKIMGLFEHHTRLLTPKKLKNIVDKLANGTLTNEEIFNDNGHGAVVLDSINPEEILIAGPNREMFKDYGKYAYPGGDVMITGCVGLYDVLKSIKGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEM0</accession>
    <name>F6BEM0_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ribulose bisphosphate carboxylase</fullName>
            <shortName evidence="1">RuBisCO</shortName>
            <ecNumber evidence="1">4.1.1.39</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rbcL</name>
        <name evidence="4" type="ordered locus">Metig_1280</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the addition of molecular CO(2) and H(2)O to ribulose 1,5-bisphosphate (RuBP), generating two molecules of 3-phosphoglycerate (3-PGA). Functions in an archaeal AMP degradation pathway, together with AMP phosphorylase and R15P isomerase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2 (2R)-3-phosphoglycerate + 2 H(+) = CO2 + D-ribulose 1,5-bisphosphate + H2O</text>
            <dbReference type="Rhea" id="RHEA:23124"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57870"/>
            <dbReference type="ChEBI" id="CHEBI:58272"/>
            <dbReference type="EC" id="4.1.1.39"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>D-ribulose 1,5-bisphosphate + O2 = (2R)-3-phosphoglycerate + 2-phosphoglycolate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:36631"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:57870"/>
            <dbReference type="ChEBI" id="CHEBI:58033"/>
            <dbReference type="ChEBI" id="CHEBI:58272"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="1">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer or homodecamer. In contrast to form I RuBisCO, the form III RuBisCO is composed solely of large subunits.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="1">Because the Archaea possessing a type III RuBisCO are all anaerobic, it is most likely that only the carboxylase activity of RuBisCO, and not the competitive oxygenase activity (by which RuBP reacts with O(2) to form one molecule of 3-phosphoglycerate and one molecule of 2-phosphoglycolate), is biologically relevant in these strains.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the RuBisCO large chain family. Type III subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.39" evidence="1"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96817.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799414.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1280"/>
    <dbReference type="EnsemblBacteria" id="AEF96817">
        <property type="protein sequence ID" value="AEF96817"/>
        <property type="gene ID" value="Metig_1280"/>
    </dbReference>
    <dbReference type="GeneID" id="10644143"/>
    <dbReference type="KEGG" id="mig:Metig_1280"/>
    <dbReference type="HOGENOM" id="CLU_031450_3_1_2"/>
    <dbReference type="OMA" id="IHGHPDG"/>
    <dbReference type="OrthoDB" id="9533at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016984">
        <property type="term" value="F:ribulose-bisphosphate carboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006196">
        <property type="term" value="P:AMP catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015977">
        <property type="term" value="P:carbon fixation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01133">
        <property type="entry name" value="RuBisCO_L_type3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033966">
        <property type="entry name" value="RuBisCO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017712">
        <property type="entry name" value="RuBisCO_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000685">
        <property type="entry name" value="RuBisCO_lsu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036376">
        <property type="entry name" value="RuBisCO_lsu_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017443">
        <property type="entry name" value="RuBisCO_lsu_fd_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036422">
        <property type="entry name" value="RuBisCO_lsu_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42704">
        <property type="entry name" value="PTHR42704"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00016">
        <property type="entry name" value="RuBisCO_large"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02788">
        <property type="entry name" value="RuBisCO_large_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00014">
        <property type="entry name" value="RuBisCO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51649">
        <property type="entry name" value="SSF51649"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54966">
        <property type="entry name" value="SSF54966"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03326">
        <property type="entry name" value="rubisco_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0120">Carbon dioxide fixation</keyword>
    <keyword evidence="1 4" id="KW-0456">Lyase</keyword>
    <keyword evidence="1" id="KW-0460">Magnesium</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RuBisCO_large_N" evidence="3">
        <location>
            <begin position="2"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="domain" description="RuBisCO_large" evidence="2">
        <location>
            <begin position="132"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="1">
        <location>
            <begin position="353"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="1">
        <location>
            <begin position="375"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="153"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="269"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="155"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" description="via carbamate group" evidence="1">
        <location>
            <position position="179"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="181"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="182"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="270"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="302"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="1">
        <location>
            <position position="309"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-carboxylysine" evidence="1">
        <location>
            <position position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02788"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEF96817.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="425" mass="47770" checksum="15C5FA76C0A0A0EA" modified="2011-07-27" version="1">MDYVDLNYTPSENDLLSCIRVKAKNLMKVANEIAAESSIGTWTKVQTMKSDVYEKLRPKVYEIEEIGEEGESKVGIIKIAYPLDAFEIENMPGTLAGIAGNIFGMKIVDGLRILDYRFPEKFVKAYRGPRYGIDGIRKLLKVEERPLLGTIVKPKVGLKTDEFAEVAYNAWIGGCDIVKDDENLVSQSFNKFEDRIFKVLELRDKAEEETGEKKIYMPNITAPYREMIKRAELAEDAGSEYVMLDVVVLGFSAVQQFREEDFKFVIHAHRAMHAAITRSKDWGITMLTLAKIYRLLGVDQLHIGTVVGKMEGERAEVCAIRDEIVLDKVEKDDKNKFFDQDWFGMNNVFPVASGGVYPKLVPEIIEILGKDVIIQAGGGIHGHPEGTVKGAIAMRAAIDAVMEGKSLEEKAEEVKELKVALEHWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEQ3</accession>
    <name>F6BEQ3_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Methyltransferase type 11</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1313</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96850.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799447.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96850">
        <property type="protein sequence ID" value="AEF96850"/>
        <property type="gene ID" value="Metig_1313"/>
    </dbReference>
    <dbReference type="GeneID" id="10644176"/>
    <dbReference type="KEGG" id="mig:Metig_1313"/>
    <dbReference type="HOGENOM" id="CLU_057700_0_0_2"/>
    <dbReference type="OMA" id="LDTCMGL"/>
    <dbReference type="OrthoDB" id="75744at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013216">
        <property type="entry name" value="Methyltransf_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08241">
        <property type="entry name" value="Methyltransf_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransf_11" evidence="1">
        <location>
            <begin position="128"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96850.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="276" mass="31571" checksum="191852532EAD2199" modified="2011-07-27" version="1">MFWITYREAQKILNAKNSVSLNLDLEKTSKTWVVEIVDNKAVFPDGSEIDLSIMEKIAKDESTVYFVRDGEVYKAAISNDGYYKLVSTIPPTIEINGIRMHRTKDTNPYKDTLEKINTIKPKKGEFILDTCMGLGYTAIECAKRGARVITIEKNPNVLEIAKINPYSEELFTNKNIEVRMGNAFDVVKEFEDGTFDAIVHDPPRFSLAGELYSEEFYRELYRILKPGGRLFHYVGNPGKKYRHKDLQRGVMERLRNVGFVDVKRVEEALGVVGKKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEQ9</accession>
    <name>F6BEQ9_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 9">4-hydroxy-tetrahydrodipicolinate synthase</fullName>
            <shortName evidence="9">HTPA synthase</shortName>
            <ecNumber evidence="4 9">4.3.3.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">dapA</name>
        <name evidence="10" type="ordered locus">Metig_1319</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 9">Catalyzes the condensation of (S)-aspartate-beta-semialdehyde [(S)-ASA] and pyruvate to 4-hydroxy-tetrahydrodipicolinate (HTPA).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>L-aspartate 4-semialdehyde + pyruvate = (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + H(+) + H2O</text>
            <dbReference type="Rhea" id="RHEA:34171"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:67139"/>
            <dbReference type="ChEBI" id="CHEBI:537519"/>
            <dbReference type="EC" id="4.3.3.7"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 9">Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; (S)-tetrahydrodipicolinate from L-aspartate: step 3/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Homotetramer; dimer of dimers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the DapA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Was originally thought to be a dihydrodipicolinate synthase (DHDPS), catalyzing the condensation of (S)-aspartate-beta-semialdehyde [(S)-ASA] and pyruvate to dihydrodipicolinate (DHDP). However, it was shown in E.coli that the product of the enzymatic reaction is not dihydrodipicolinate but in fact (4S)-4-hydroxy-2,3,4,5-tetrahydro-(2S)-dipicolinic acid (HTPA), and that the consecutive dehydration reaction leading to DHDP is not spontaneous but catalyzed by DapB.</text>
    </comment>
    <dbReference type="EC" id="4.3.3.7" evidence="4 9"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96856.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799453.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1319"/>
    <dbReference type="EnsemblBacteria" id="AEF96856">
        <property type="protein sequence ID" value="AEF96856"/>
        <property type="gene ID" value="Metig_1319"/>
    </dbReference>
    <dbReference type="GeneID" id="10644182"/>
    <dbReference type="KEGG" id="mig:Metig_1319"/>
    <dbReference type="HOGENOM" id="CLU_049343_7_1_2"/>
    <dbReference type="OMA" id="GMDACVP"/>
    <dbReference type="OrthoDB" id="65776at2157"/>
    <dbReference type="UniPathway" id="UPA00034">
        <property type="reaction ID" value="UER00017"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008675">
        <property type="term" value="F:2-dehydro-3-deoxy-phosphogluconate aldolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008840">
        <property type="term" value="F:4-hydroxy-tetrahydrodipicolinate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019877">
        <property type="term" value="P:diaminopimelate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009089">
        <property type="term" value="P:lysine biosynthetic process via diaminopimelate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00950">
        <property type="entry name" value="DHDPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00418">
        <property type="entry name" value="DapA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005263">
        <property type="entry name" value="DapA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002220">
        <property type="entry name" value="DapA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020625">
        <property type="entry name" value="Schiff_base-form_aldolases_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020624">
        <property type="entry name" value="Schiff_base-form_aldolases_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12128">
        <property type="entry name" value="PTHR12128"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00701">
        <property type="entry name" value="DHDPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001365">
        <property type="entry name" value="DHDPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00146">
        <property type="entry name" value="DHPICSNTHASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01130">
        <property type="entry name" value="DHDPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00674">
        <property type="entry name" value="dapA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00665">
        <property type="entry name" value="DHDPS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00666">
        <property type="entry name" value="DHDPS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 9" id="KW-0220">Diaminopimelate biosynthesis</keyword>
    <keyword evidence="9" id="KW-0456">Lyase</keyword>
    <keyword evidence="7 9" id="KW-0457">Lysine biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 9" id="KW-0704">Schiff base</keyword>
    <feature type="active site" description="Proton donor/acceptor" evidence="9">
        <location>
            <position position="131"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with substrate" evidence="9">
        <location>
            <position position="160"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="43"/>
        </location>
        <ligand>
            <name>pyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="200"/>
        </location>
        <ligand>
            <name>pyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
        </ligand>
    </feature>
    <feature type="site" description="Part of a proton relay during catalysis" evidence="9">
        <location>
            <position position="42"/>
        </location>
    </feature>
    <feature type="site" description="Part of a proton relay during catalysis" evidence="9">
        <location>
            <position position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AEF96856.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="289" mass="31572" checksum="075221B018F02308" modified="2011-07-27" version="1">MRGVFPAIITPFKNGEIDFEGLEKNINFLIENGVSGIVAVGTTGESPTLSHEEHKKVIEKAVEIVDGRVTVIAGAGSNSTAEAVELSQFAEDVGADAVLSITPYYNKPTQEGLKKHFGTIAESINLPIILYNVPSRTAVNLEPSTVKYLAEEYSNITAIKEANPNLSQVSEIIRECNITVLSGNDELTLPIIALGGKGVISVVANIVPKEFVEMVDYALKGEFDKAREIHYKLYPLMKAMFIETNPIPIKTAMNMLGMPSGELRLPLCKMKEENKKKLEEVLKNLGLLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-06-02" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>F6BER9</accession>
    <name>F6BER9_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1329</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96866.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799463.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96866">
        <property type="protein sequence ID" value="AEF96866"/>
        <property type="gene ID" value="Metig_1329"/>
    </dbReference>
    <dbReference type="GeneID" id="10644192"/>
    <dbReference type="KEGG" id="mig:Metig_1329"/>
    <dbReference type="HOGENOM" id="CLU_040995_0_0_2"/>
    <dbReference type="OMA" id="YPKTGHE"/>
    <dbReference type="OrthoDB" id="9944at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="9"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="41"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="70"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96866.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="513" mass="59603" checksum="5369376C299C11FC" modified="2011-07-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>F6BET4</accession>
    <name>F6BET4_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">O-phosphoserine--tRNA(Cys) ligase</fullName>
            <shortName evidence="1">O-phosphoserine--tRNA ligase</shortName>
            <ecNumber evidence="1">6.1.1.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Non-canonical O-phosphoseryl-tRNA(Cys) synthetase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">O-phosphoseryl-tRNA(Cys) synthetase</fullName>
            <shortName evidence="1">SepRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">sepS</name>
        <name evidence="3" type="ordered locus">Metig_1346</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the attachment of O-phosphoserine (Sep) to tRNA(Cys).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + O-phospho-L-serine + tRNA(Cys) = AMP + diphosphate + O-phospho-L-seryl-tRNA(Cys)</text>
            <dbReference type="Rhea" id="RHEA:25678"/>
            <dbReference type="Rhea" id="RHEA-COMP:9661"/>
            <dbReference type="Rhea" id="RHEA-COMP:9719"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57524"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78551"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.27"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homotetramer. Interacts with SepCysS.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-II aminoacyl-tRNA synthetase family. O-phosphoseryl-tRNA(Cys) synthetase subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.27" evidence="1"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96881.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799478.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1346"/>
    <dbReference type="EnsemblBacteria" id="AEF96881">
        <property type="protein sequence ID" value="AEF96881"/>
        <property type="gene ID" value="Metig_1346"/>
    </dbReference>
    <dbReference type="GeneID" id="10644209"/>
    <dbReference type="KEGG" id="mig:Metig_1346"/>
    <dbReference type="HOGENOM" id="CLU_506822_0_0_2"/>
    <dbReference type="OMA" id="RSHMTSG"/>
    <dbReference type="OrthoDB" id="6499at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043816">
        <property type="term" value="F:phosphoserine-tRNA(Cys) ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043039">
        <property type="term" value="P:tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01674">
        <property type="entry name" value="Sep_tRNA_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005246">
        <property type="entry name" value="O-Pseryl-tRNA(Cys)_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002319">
        <property type="entry name" value="Phenylalanyl-tRNA_Synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041590">
        <property type="entry name" value="SepRS_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11538:SF38">
        <property type="entry name" value="PTHR11538:SF38"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18006">
        <property type="entry name" value="SepRS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01409">
        <property type="entry name" value="tRNA-synt_2d"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="SSF55681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00470">
        <property type="entry name" value="sepS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0436">Ligase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="1" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AA_TRNA_LIGASE_II" evidence="2">
        <location>
            <begin position="175"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="1">
        <location>
            <begin position="187"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="1">
        <location>
            <begin position="232"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="1">
        <location>
            <begin position="274"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="318"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF96881.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="538" mass="62118" checksum="37B9962072CD9CFD" modified="2011-07-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEW3</accession>
    <name>F6BEW3_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Teichoic-acid-transporting ATPase</fullName>
            <ecNumber evidence="5">3.6.3.40</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Metig_0139</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.6.3.40" evidence="5"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95699.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0139"/>
    <dbReference type="EnsemblBacteria" id="AEF95699">
        <property type="protein sequence ID" value="AEF95699"/>
        <property type="gene ID" value="Metig_0139"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0139"/>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_2"/>
    <dbReference type="OMA" id="RLICRVA"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="8"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="40"/>
            <end position="47"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEF95699.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="278" mass="32349" checksum="9965A54C141A2782" modified="2011-07-27" version="1">MGRIMKKVKVRNLVKYFGDKKAIDNISFEVYEGEIFGLLGHNGAGKTTTLRIIAGIINDYEGEVEVNGKIGYLPEERGLYKDERVGEVLTFFGELAGMEKEEIKNQIDYWLKKLNIYKYKNQKVKTLSKGNQQKVQFIISVLHNPDIIILDEPFSGLDIFNLNLLKEILFELKEMGKSIILSTHQLEKIERFCDRVLVLKNGKVIHYGRIDEICHKKVAYVEYIKDGKLIKQNLPHEEAILLIKNEEILRNLIKFEVRSSLEDLFLKETFKKLNENQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEX4</accession>
    <name>F6BEX4_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0310 protein Metig_0150</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_0150</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0310 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95710.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798319.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0150"/>
    <dbReference type="EnsemblBacteria" id="AEF95710">
        <property type="protein sequence ID" value="AEF95710"/>
        <property type="gene ID" value="Metig_0150"/>
    </dbReference>
    <dbReference type="GeneID" id="10642985"/>
    <dbReference type="KEGG" id="mig:Metig_0150"/>
    <dbReference type="HOGENOM" id="CLU_148445_0_0_2"/>
    <dbReference type="OMA" id="EKNVWGV"/>
    <dbReference type="OrthoDB" id="92589at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00771">
        <property type="entry name" value="UPF0310"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002740">
        <property type="entry name" value="EVE_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022996">
        <property type="entry name" value="UPF0310"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01878">
        <property type="entry name" value="EVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="SSF88697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EVE" evidence="2">
        <location>
            <begin position="2"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF95710.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="144" mass="17239" checksum="78FFF58FF581548D" modified="2011-07-27" version="1">MTYWLCITNEDNWKIIKDKKIWGVAERHKNTINKVKVGDKLIIYEIQRSGKDYKPPYIRGVYEVVSEVYKDSTKVFKPTPRNPNEKFPWRVKLKEVKVFEPPVNFKDLIPKLKFITNKKKWSGHIMGKAMREIPEEDYRLIVEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>F6BF30</accession>
    <name>F6BF30_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Phenylalanine--tRNA ligase alpha subunit</fullName>
            <ecNumber evidence="7">6.1.1.20</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Phenylalanyl-tRNA synthetase alpha subunit</fullName>
            <shortName evidence="7">PheRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">pheS</name>
        <name evidence="9" type="ordered locus">Metig_1365</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + H(+) + L-phenylalanyl-tRNA(Phe)</text>
            <dbReference type="Rhea" id="RHEA:19413"/>
            <dbReference type="Rhea" id="RHEA-COMP:9668"/>
            <dbReference type="Rhea" id="RHEA-COMP:9699"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58095"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78531"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.20"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="7">Binds 2 magnesium ions per tetramer.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Tetramer of two alpha and two beta subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha subunit type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.20" evidence="7"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96900.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1365"/>
    <dbReference type="EnsemblBacteria" id="AEF96900">
        <property type="protein sequence ID" value="AEF96900"/>
        <property type="gene ID" value="Metig_1365"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_1365"/>
    <dbReference type="HOGENOM" id="CLU_025086_2_2_2"/>
    <dbReference type="OMA" id="QIEGWVM"/>
    <dbReference type="OrthoDB" id="22747at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004826">
        <property type="term" value="F:phenylalanine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006432">
        <property type="term" value="P:phenylalanyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00282">
        <property type="entry name" value="Phe_tRNA_synth_alpha2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004529">
        <property type="entry name" value="Phe-tRNA-synth_IIc_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022917">
        <property type="entry name" value="Phe_tRNA_ligase_alpha_bac/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002319">
        <property type="entry name" value="Phenylalanyl-tRNA_Synthase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01409">
        <property type="entry name" value="tRNA-synt_2d"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="SSF55681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00468">
        <property type="entry name" value="pheS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7 9" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="4 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 7" id="KW-0436">Ligase</keyword>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 7" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AA_TRNA_LIGASE_II" evidence="8">
        <location>
            <begin position="233"/>
            <end position="492"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AEF96900.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="492" mass="57646" checksum="2D4B190EB46C3A39" modified="2011-07-27" version="1">MDEKRLLKVFQETRKEIMDVDELSKHIEKEKIMRTALWLSGKELVDIIENKESIAILTEEGKKVLEKGLPERRIAKYLKENDLTSIPIKDLKNILDKDEINAALGNLKKKGIAKIEKGNIIFESLDYEDEEEILLAKLKENPHLSLYNEKERKIIENLKKRGFIKIDEKVERKIKLTEKGKEYIKNPIEIKEEITQLTREDIIGGRWREAYIRPYDVKIPVEEVYPAKMHPLTRIIREVKEVLISMGFKEIKSPIVETEFWNFDVLFEPQDHPAREMQDTFFLKYPTEGDVPEDLLKKVKSIHEDGTIDGVKVSEGWKYKFDENVSRRTVLRTHTTASSIRYLASLSEEEKEKPHKVFCIDRVFRNEAIDYKHLPEFYQCEGIIMDDNVTFDNLVGILKEFLNRLGFEKVRFRPAYFPFTEPSLEAEVYLEGKGWLELLGAGMFRPEVLEPLGIKKPVLAWGIGFSRLAMLKLGLNDIRDLHKNDLDWLKRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>F6BF41</accession>
    <name>F6BF41_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="3">NurA domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_1376</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96911.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799507.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1376"/>
    <dbReference type="EnsemblBacteria" id="AEF96911">
        <property type="protein sequence ID" value="AEF96911"/>
        <property type="gene ID" value="Metig_1376"/>
    </dbReference>
    <dbReference type="GeneID" id="10644241"/>
    <dbReference type="KEGG" id="mig:Metig_1376"/>
    <dbReference type="HOGENOM" id="CLU_056881_0_0_2"/>
    <dbReference type="OMA" id="YILKKSH"/>
    <dbReference type="OrthoDB" id="57919at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018977">
        <property type="entry name" value="NurA_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09376">
        <property type="entry name" value="NurA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00933">
        <property type="entry name" value="NurA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NurA" evidence="2">
        <location>
            <begin position="42"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="247"/>
            <end position="310"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF96911.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="382" mass="44620" checksum="55B629276D65AD36" modified="2011-07-27" version="1">MDLSIILKKKEIIKKKIEELGNKNLNVNDYWVECDFDNPEDLIFGGGDGSFNNIDYISYSLYMVGSISYLHKVGEKIEKAKSSYDIDIIQPYVSVRDRLRLYMLTMELKTALWTLENKNVDYYLVDGSLYSLLIQTHTYGSKYVDVEEIYEEYSKEIKNSIEEEIKSGNICIASNNIEIEDKNEKIVVEQVEYIITLLKLLKKHKDKLIGISKTSKMNIYFKNALMPDIAVFSKNTKNSGYSTPISLKMLKEEMEKLKEKIDNEDNKNKKDELKREYNYIRGIVHNIEYINRFEHALDNLYLQFVRLENNKGILGISSLKPIDEQVLSSLKEISVNGYPYILKKSHETVEITDDKLEMCAKILNINEPIDRYLLKKQKRMDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-06-02" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>F6BF50</accession>
    <name>F6BF50_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1385</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96920.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96920">
        <property type="protein sequence ID" value="AEF96920"/>
        <property type="gene ID" value="Metig_1385"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_1385"/>
    <dbReference type="HOGENOM" id="CLU_204983_0_0_2"/>
    <dbReference type="OMA" id="IFCAFGR"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032820">
        <property type="entry name" value="ATPase_put"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09527">
        <property type="entry name" value="ATPase_gene1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="33"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96920.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="61" mass="6889" checksum="DF2EA431007E56E3" modified="2011-07-27" version="1">MVMKIRDIALEFVSCIVIGIVVGYIIGAQTNNMIYVVIGLLIGILAGISRFIKFIRNYKQW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F6BF78</accession>
    <name>F6BF78_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ABC-type transporter, periplasmic subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1413</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96948.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1413"/>
    <dbReference type="EnsemblBacteria" id="AEF96948">
        <property type="protein sequence ID" value="AEF96948"/>
        <property type="gene ID" value="Metig_1413"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_1413"/>
    <dbReference type="HOGENOM" id="CLU_017028_7_5_2"/>
    <dbReference type="OMA" id="YIAYNVM"/>
    <dbReference type="OrthoDB" id="6658at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030678">
        <property type="entry name" value="Peptide/Ni-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039424">
        <property type="entry name" value="SBP_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000914">
        <property type="entry name" value="SBP_5_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30290">
        <property type="entry name" value="PTHR30290"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00496">
        <property type="entry name" value="SBP_bac_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002741">
        <property type="entry name" value="MppA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SBP_bac_5" evidence="1">
        <location>
            <begin position="84"/>
            <end position="428"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96948.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="528" mass="60661" checksum="AE1F8409B0AEC69A" modified="2011-07-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-09-29" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>F6BA68</accession>
    <name>F6BA68_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="3">AAA ATPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_0201</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the archaeal ATPase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95758.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798367.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF95758">
        <property type="protein sequence ID" value="AEF95758"/>
        <property type="gene ID" value="Metig_0201"/>
    </dbReference>
    <dbReference type="GeneID" id="10643036"/>
    <dbReference type="KEGG" id="mig:Metig_0201"/>
    <dbReference type="HOGENOM" id="CLU_068608_0_0_2"/>
    <dbReference type="OMA" id="EILEFML"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011579">
        <property type="entry name" value="ATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01637">
        <property type="entry name" value="ATPase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA" evidence="2">
        <location>
            <begin position="21"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF95758.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="356" mass="42223" checksum="D5CD65FC64712395" modified="2011-07-27" version="1">MRFFNREREINEVLHILSREPDDIYFVYGPMNSGKTTLIKHIVEDKLGYEYKVFYINFRTYLISEKREFIEAIFTTKKGDLFDKIKDKNEVLRLITKGAKVLTGVPIPEVEFDKLFDEKINDAFQYLNNMLLTVKKNGKKPILILDELQMIKDVVLNGQKYLLKELFQFLVSLTKEQHLCHVFCLSSDSLFIEYVYNTGDLEGRVKYLLVDDFDKETALKFMDFLSEDILGKKLPDEDKKLIYSYVGGKSKDIKYVVEESSFKNLNEVLEYMLKDKTSKLKKLLVMVKNNRFERIDYEKVVNALRLFKENYEINEYQIDEDTKEFLIERNVLFLNPIEESLKPQSFLIWNAIKRVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>F6BA90</accession>
    <name>F6BA90_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="3">4Fe-4S ferredoxin iron-sulfur binding domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_0223</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95780.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798389.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0223"/>
    <dbReference type="EnsemblBacteria" id="AEF95780">
        <property type="protein sequence ID" value="AEF95780"/>
        <property type="gene ID" value="Metig_0223"/>
    </dbReference>
    <dbReference type="GeneID" id="10643058"/>
    <dbReference type="KEGG" id="mig:Metig_0223"/>
    <dbReference type="HOGENOM" id="CLU_582186_0_0_2"/>
    <dbReference type="OMA" id="CAIHCPR"/>
    <dbReference type="OrthoDB" id="11187at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00037">
        <property type="entry name" value="Fer4"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12800">
        <property type="entry name" value="Fer4_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12838">
        <property type="entry name" value="Fer4_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13187">
        <property type="entry name" value="Fer4_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="12"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="2">
        <location>
            <begin position="34"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="2">
        <location>
            <begin position="66"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="2">
        <location>
            <begin position="96"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="2">
        <location>
            <begin position="171"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="2">
        <location>
            <begin position="202"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="2">
        <location>
            <begin position="220"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="2">
        <location>
            <begin position="268"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="2">
        <location>
            <begin position="318"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="2">
        <location>
            <begin position="354"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="2">
        <location>
            <begin position="383"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="2">
        <location>
            <begin position="422"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="2">
        <location>
            <begin position="447"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="147"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF95780.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="482" mass="53807" checksum="E201F6B7412E97B9" modified="2011-07-27" version="1">MIITILEKCRPEEKCETCPFKTKSKCMEVCPTDAIMLLDNKAFSCITCGTCARECPNNAIRKNEFGGYYVDRKRCTGCGICANVCPIGIIKMVEKEGKRFPMGICIMCDLCVEACPYNARVSGVECIDLKRTAYAEKYATKIFNIIKMIKKEEIEEIEEESKKTKNNKIRVSIKIDKEKCVGCGKCSYLCPRETIIPNKDVDACTSCNICGDVCPNDAIENGTIKEDGNCVLCLRCVNSCPKNALKVENFKVVKIKEDKRIINGKTKGFAVYSLRHCINCGLCVDNCPSGALKKEDDKILYDASICWKCNKCVDVCPQKVRVNKGDYISGGCSLCGICVEMCPKNAISIEKIGWKNIRDENCITCGTCANVCPNDAVTIKINSKEIIFNNNCVMCETCSIHCPRDVIPNTTGYKKVVDRENSFIRTDFAFCTRCGLCTKICPNDAIKDGEIDIEKCEFCSACVNICPTHAIYIYRTWKNKEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-02-10" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAB3</accession>
    <name>F6BAB3_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Integrase catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_0246</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95803.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF95803">
        <property type="protein sequence ID" value="AEF95803"/>
        <property type="gene ID" value="Metig_0246"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0246"/>
    <dbReference type="HOGENOM" id="CLU_2044423_0_0_2"/>
    <dbReference type="OMA" id="ADCFLMF"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00665">
        <property type="entry name" value="rve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="2">
        <location>
            <begin position="167"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF95803.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="291" mass="34204" checksum="8164146D09117B19" modified="2011-07-27" version="1">MVSRNYFASVGNKKRNVNNKNNMKGAKLNDKKIRKIIRWKEKGFETSRIAKKVGVTPRRVQQIWKEYRETGEIPKIKRRGRKPKKISKEEIDLVLKAVEEYKGTSAVHLEKYIENKHNIHIPHNRIYTILKEFDLIQKGKKKKKELDENLRFSLLACYASEKPKRFSASKPNEMWQMDFKIVDVGGVRYNLLLIIDDHSRFILHAGIYEEATTDVVISALEECFEKHGTPKKILTDNGTQFVPARGGISRFQKFLMEREILHIKTSIRHPQTIGKVERINREVEYRLDKFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAF9</accession>
    <name>F6BAF9_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">S_layer_C domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_1437</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96972.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799568.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1437"/>
    <dbReference type="EnsemblBacteria" id="AEF96972">
        <property type="protein sequence ID" value="AEF96972"/>
        <property type="gene ID" value="Metig_1437"/>
    </dbReference>
    <dbReference type="GeneID" id="10644310"/>
    <dbReference type="KEGG" id="mig:Metig_1437"/>
    <dbReference type="HOGENOM" id="CLU_890304_0_0_2"/>
    <dbReference type="OMA" id="NYTINHA"/>
    <dbReference type="OrthoDB" id="4820at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022651">
        <property type="entry name" value="S_layer_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006454">
        <property type="entry name" value="S_layer_MJ"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05124">
        <property type="entry name" value="S_layer_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01564">
        <property type="entry name" value="S_layer_MJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="S_layer_C" evidence="2">
        <location>
            <begin position="186"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="154"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF96972.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="320" mass="35695" checksum="C22589A98006C9CC" modified="2011-07-27" version="1">MKYIYVMLGMMLLLLIPMVAGEVKEIPQGNVEINIIQYNVSFNYTLEEDKNLTILELNTSKEGDIVAYFDNKPLFLYRPSNDSSAKTYNISLLNGTIAIVGVNASNYNKIEETTNCTFVGWNKTLGYAFFLFDNIGESKSGIIKLKYEPQASTTTTTTTSYSSGGGGGSHHITTTEQYPGVAEDIKSEKIKEMVYKAKIIIGSEVDNNLSAKYLKNTTELVNQSLEIKEDCILIGGPVANPVVKKWLWAFPVKITNDYPGKNRGVIEKQMINDHIVILLAGSDRWGTKAAVEYFKTLDDIPDEPIFVEWKDGKAVKIEKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAG2</accession>
    <name>F6BAG2_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_1440</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96975.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799571.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1440"/>
    <dbReference type="EnsemblBacteria" id="AEF96975">
        <property type="protein sequence ID" value="AEF96975"/>
        <property type="gene ID" value="Metig_1440"/>
    </dbReference>
    <dbReference type="GeneID" id="10644313"/>
    <dbReference type="KEGG" id="mig:Metig_1440"/>
    <dbReference type="HOGENOM" id="CLU_119412_2_2_2"/>
    <dbReference type="OMA" id="IYYGTKF"/>
    <dbReference type="OrthoDB" id="120594at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002878">
        <property type="entry name" value="DUF35_OB-fold_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022002">
        <property type="entry name" value="DUF35_Znr_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12172">
        <property type="entry name" value="DUF35_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01796">
        <property type="entry name" value="OB_aCoA_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF35_N" evidence="2">
        <location>
            <begin position="8"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="domain" description="OB_aCoA_assoc" evidence="1">
        <location>
            <begin position="47"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF96975.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="131" mass="15232" checksum="FC234EAD36009D78" modified="2011-07-27" version="1">MVVRTWRHIRERYNLIGVKCKNCGTVYFPSRILCPKCRRKTQMEEIKLSGKGKVYTYSVVHTPPKEFEKIAPYIIAIVELEEGPKVTAQIVDCDKDEIYIGMPVEAVFRRIKEDGEDGIISYGYKFRPVKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAG5</accession>
    <name>F6BAG5_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Propanoyl-CoA C-acyltransferase</fullName>
            <ecNumber evidence="3">2.3.1.176</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_1443</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.3.1.176" evidence="3"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96978.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799574.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1443"/>
    <dbReference type="EnsemblBacteria" id="AEF96978">
        <property type="protein sequence ID" value="AEF96978"/>
        <property type="gene ID" value="Metig_1443"/>
    </dbReference>
    <dbReference type="GeneID" id="10644316"/>
    <dbReference type="KEGG" id="mig:Metig_1443"/>
    <dbReference type="HOGENOM" id="CLU_035425_4_0_2"/>
    <dbReference type="OMA" id="PSLYAMM"/>
    <dbReference type="OrthoDB" id="44046at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033814">
        <property type="term" value="F:propanoyl-CoA C-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050632">
        <property type="term" value="F:propionyl-CoA C2-trimethyltridecanoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002155">
        <property type="entry name" value="Thiolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020617">
        <property type="entry name" value="Thiolase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020616">
        <property type="entry name" value="Thiolase_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02803">
        <property type="entry name" value="Thiolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00108">
        <property type="entry name" value="Thiolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000429">
        <property type="entry name" value="Ac-CoA_Ac_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="SSF53901"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Thiolase_N" evidence="1">
        <location>
            <begin position="4"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="domain" description="Thiolase_C" evidence="2">
        <location>
            <begin position="258"/>
            <end position="358"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF96978.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="392" mass="42200" checksum="554E76D97AAEABE3" modified="2011-07-27" version="1">MRDVAIIGYGQTKFGELWEKSFRELIVEAGIKAINEANVDGKDIDAMYIGNMSGGLFVGQEHIAALIADHAGLNPIPCTRVEAACASGSLALRNAVLSVASGHHDVVLVGGVEKMTDVSDATSAIASASDQEWEAFFGATFPSLYAMMARRYMYEYGLTIEELSMWSVIMHENASKNKYAQFPFKVTLEQVINASPVAEPLTLMHCSPISDGASALIVCEAEKAKEFVNSDDIIYIKASTQASDTMALHDRESMTTLKAAKVASEKAYKMAGISPNKIDVAEVHDCFAINGLILIEDLGFCKKGEAGKIVYEGKIKIDYDEFPTVNPSGGLKAAGHALGATGIRQVGEIYWQLKQDKECKDRQASIKHGYGITANVGGTGGTVCVHILSDKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAG8</accession>
    <name>F6BAG8_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0219 protein Metig_1446</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metig_1446</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the thiolase-like superfamily. UPF0219 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96981.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799577.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1446"/>
    <dbReference type="EnsemblBacteria" id="AEF96981">
        <property type="protein sequence ID" value="AEF96981"/>
        <property type="gene ID" value="Metig_1446"/>
    </dbReference>
    <dbReference type="GeneID" id="10644319"/>
    <dbReference type="KEGG" id="mig:Metig_1446"/>
    <dbReference type="HOGENOM" id="CLU_039592_7_0_2"/>
    <dbReference type="OMA" id="YAKHKGK"/>
    <dbReference type="OrthoDB" id="12601at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016746">
        <property type="term" value="F:acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01409">
        <property type="entry name" value="UPF0219"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013747">
        <property type="entry name" value="ACP_syn_III_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004656">
        <property type="entry name" value="FabH-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013528">
        <property type="entry name" value="HMG_CoA_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08541">
        <property type="entry name" value="ACP_syn_III_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01154">
        <property type="entry name" value="HMG_CoA_synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="SSF53901"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00748">
        <property type="entry name" value="HMG_CoA_syn_Arc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HMG_CoA_synt_N" evidence="2">
        <location>
            <begin position="2"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="domain" description="ACP_syn_III_C" evidence="3">
        <location>
            <begin position="222"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF08541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEF96981.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="348" mass="37579" checksum="CD7CBC33637C6309" modified="2011-07-27" version="1">MKVGIVGYGAYIPKYRIKVEEIARVWGKNPESIKKGLIINEKSVPGPDEDTATIAVAAARNALKRAGIEGKRIGAIYVGSESHPYAVKPTSTIVAEAIEATPDLTAADLEFACKAGTAGIQMCMGLVGSGIIEYGLAIGADTAQGAPGDALEYTAAAGGAAYIIGNKKDELIAIFNGTYSFTTDTPDFWRREGQPYPRHGGRFTGEPAYFKHVLSAARGLMEKMDTTPEDYDYCVFHQPNGKFYIKAAKTLGFKEEQYKNGLLTPYLGNTYSGAVPLGLSNILDNAKEGDRILAVSYGSGAGSDAFDLTVTKRIEEVKDKAPKTMEMLNDKIYIDYATYVKFRGKIKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAH0</accession>
    <name>F6BAH0_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">TraB family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1448</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96983.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799579.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1448"/>
    <dbReference type="EnsemblBacteria" id="AEF96983">
        <property type="protein sequence ID" value="AEF96983"/>
        <property type="gene ID" value="Metig_1448"/>
    </dbReference>
    <dbReference type="GeneID" id="10644321"/>
    <dbReference type="KEGG" id="mig:Metig_1448"/>
    <dbReference type="HOGENOM" id="CLU_032780_1_0_2"/>
    <dbReference type="OMA" id="WMLSYVQ"/>
    <dbReference type="OrthoDB" id="52124at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14726">
        <property type="entry name" value="TraB_PrgY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002816">
        <property type="entry name" value="TraB/PrgY/GumN_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005230">
        <property type="entry name" value="TraB_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046345">
        <property type="entry name" value="TraB_PrgY-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21530">
        <property type="entry name" value="PTHR21530"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01963">
        <property type="entry name" value="TraB_PrgY_gumN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00261">
        <property type="entry name" value="traB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="247"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="280"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="304"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="363"/>
            <end position="382"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96983.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="388" mass="43523" checksum="865BDB9383311589" modified="2011-07-27" version="1">MKHIKINNGFNECNIYLIGTAHVSEESVRKVEEAIININPDVVAVELDRERFLAIMGNNDNMDNIDIKRVIKEGRVGIFLLHMILSYFQKKIGEELGVKPGSEMKKAIEIAIQHQKPISLIDRQINITLTRLLNKMSLKEKINFFLSLFEENGEIDIDNKSINDMVNNADELVLLLKDISPTIYEVLVDERDRYMAKNLYELSKGNEKIVAVVGAGHIRGIINYLKKLEKGEDIDLNELNMTKKSRIWKYIGKIISVAIVLVILYGFYSISSNIDALKKLTLEWVIINGFLSALGVVIARGHVISALVAFLSAPITSLIPFVGAGWIAGLAELKFREIKKSDIIALFHANSVKELLNNNLMRILLVAALANLGSAIGTLYFIPKFLGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAK9</accession>
    <name>F6BAK9_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcriptional regulator, MarR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_1488</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97022.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799617.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1488"/>
    <dbReference type="EnsemblBacteria" id="AEF97022">
        <property type="protein sequence ID" value="AEF97022"/>
        <property type="gene ID" value="Metig_1488"/>
    </dbReference>
    <dbReference type="GeneID" id="10644361"/>
    <dbReference type="KEGG" id="mig:Metig_1488"/>
    <dbReference type="HOGENOM" id="CLU_637154_0_0_2"/>
    <dbReference type="OMA" id="QVYIIVP"/>
    <dbReference type="OrthoDB" id="52593at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="193"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="211"/>
            <end position="360"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF97022.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="430" mass="49561" checksum="48C240917921703A" modified="2011-07-27" version="1">MLFLNTGFGISFEKYEVVCDVDANNNVHETITLLIFNNNSEDIKDITYIVPQTLKNLEVYSDRGVKSYSAKLIEGATEIYIELENPIKKGERGEIKLEFDSNNLVWDKENGEKLLSISVPAVKSKFELIIKLPPGAAVVSPQGFLSITPQGYVVDTDGKRIVIKWEKVLENEKSFTATVAYAIITKPITNNTLTYGFVGVSLLLMISIIFLVITTRKNKLYKNKISEMQNEIENLSKILEKKDEEVKKLNEINNLLNNDLKNVNKNIEAYKKEILEKDNEIEKIKEEYEKCLRKIEELKKELSKIDEIKDELEAYKELSERYKKELETLKDSINKKDEYIKMLENKIKEYESNKRDILMNVLTDEEKEIIDLIAKHGSITQKEIVEITGMTKPKVSRLVSDLEQRGIIKKIKIGRINKLILSEDVLKEND</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAL4</accession>
    <name>F6BAL4_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Transcription factor CBF/NF-Y/histone domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Metig_1493</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Binds and compact DNA (95 to 150 base pairs) to form nucleosome-like structures that contain positive DNA supercoils. Increases the resistance of DNA to thermal denaturation (in vitro).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the archaeal histone HMF family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97027.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007044809.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1493"/>
    <dbReference type="EnsemblBacteria" id="AEF97027">
        <property type="protein sequence ID" value="AEF97027"/>
        <property type="gene ID" value="Metig_1493"/>
    </dbReference>
    <dbReference type="GeneID" id="10644366"/>
    <dbReference type="KEGG" id="mig:Metig_1493"/>
    <dbReference type="HOGENOM" id="CLU_192667_0_0_2"/>
    <dbReference type="OMA" id="ELPVAPC"/>
    <dbReference type="OrthoDB" id="127272at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003958">
        <property type="entry name" value="CBFA_NFYB_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00808">
        <property type="entry name" value="CBFD_NFYB_HMF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="SSF47113"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0158">Chromosome</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBFD_NFYB_HMF" evidence="7">
        <location>
            <begin position="2"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEF97027.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="67" mass="7174" checksum="3D6BCF6167CF8C33" modified="2011-07-27" version="1">MAELPVAPVVRILKKAGAERVSEEAGKFFAEALEEIAMNIAQEAAALAKHAGRKTVKVEDIKMALRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAQ5</accession>
    <name>F6BAQ5_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Histidine triad (HIT) protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_0313</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95869.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798478.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0313"/>
    <dbReference type="EnsemblBacteria" id="AEF95869">
        <property type="protein sequence ID" value="AEF95869"/>
        <property type="gene ID" value="Metig_0313"/>
    </dbReference>
    <dbReference type="GeneID" id="10643148"/>
    <dbReference type="KEGG" id="mig:Metig_0313"/>
    <dbReference type="HOGENOM" id="CLU_056776_3_2_2"/>
    <dbReference type="OMA" id="SDCLFCK"/>
    <dbReference type="OrthoDB" id="93754at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01277">
        <property type="entry name" value="HINT_subgroup"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.428.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039384">
        <property type="entry name" value="HINT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019808">
        <property type="entry name" value="Histidine_triad_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001310">
        <property type="entry name" value="Histidine_triad_HIT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011146">
        <property type="entry name" value="HIT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036265">
        <property type="entry name" value="HIT-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23089">
        <property type="entry name" value="PTHR23089"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01230">
        <property type="entry name" value="HIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00332">
        <property type="entry name" value="HISTRIAD"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54197">
        <property type="entry name" value="SSF54197"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00892">
        <property type="entry name" value="HIT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51084">
        <property type="entry name" value="HIT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HIT" evidence="2">
        <location>
            <begin position="3"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Histidine triad motif" evidence="1">
        <location>
            <begin position="94"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF95869.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="129" mass="14897" checksum="3C8BF0819576080C" modified="2011-07-27" version="1">MCIFCKIVNKEIPAKIVYEDEDTMAFLDINPRNKGHTLVIPKKHYETLDEMPDEELAKLMKAVKKVVEILKPLNFDGYNIINNNKPAAGQEVPHVHFHIIPRYQNDGDVVKFGEVKKIDLDEVEKIIKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAR4</accession>
    <name>F6BAR4_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Formylmethanofuran dehydrogenase subunit B</fullName>
            <ecNumber evidence="2">1.2.99.5</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0322</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.2.99.5" evidence="2"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95878.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0322"/>
    <dbReference type="KEGG" id="mig:Metig_0322"/>
    <dbReference type="HOGENOM" id="CLU_034348_0_0_2"/>
    <dbReference type="OMA" id="VCGASCD"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018493">
        <property type="term" value="F:formylmethanofuran dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015948">
        <property type="term" value="P:methanogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02761">
        <property type="entry name" value="MopB_FmdB-FwdB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016457">
        <property type="entry name" value="Formylmethanofuran_DH_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006656">
        <property type="entry name" value="Mopterin_OxRdtase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00384">
        <property type="entry name" value="Molybdopterin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005646">
        <property type="entry name" value="FwdB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03129">
        <property type="entry name" value="one_C_dehyd_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0711">Selenium</keyword>
    <keyword evidence="2" id="KW-0712">Selenocysteine</keyword>
    <feature type="domain" description="Molybdopterin" evidence="1">
        <location>
            <begin position="51"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="non-standard amino acid" description="Selenocysteine" evidence="2">
        <location>
            <position position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF95878.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="448" mass="50216" checksum="54D9EA776A5371E6" modified="2011-07-27" version="1">MKVFKGITCPVCGMACDDIEVLYDEENQKIIVRNVCREGAPKFQEIVSPHRIREPMIKKNGEFVKVSWEEAINKAAEILANAKRPLLFMGAETSAEAHIVGLHMAEYLGGVADSNSTIUHGPTLMGVQEAGLPGCTAGEVKNRADLIIFWGCNPMHSHPRHMSRYSVFARGYWTERGRKDRKIVVVDPRKTDTAKLADLHVQLKPNTDYEMFSALLTVLRGKRPHHSIEKITGVPIETIEEMVELMKNSKFVKIYGGLGLPASRGGHRNIEMVLNLVKELNKYTKCAIGALRGHCNVNGFNQIASYMYGYPYGLDFARGYPRYNPGEFTFADLLREREIDAILVVAAGIVEHTPKDCAKYLNEIPMVCIDIAPGPTPYASDVVLPGVIDGMECGATFYRFDGLTLYAKPFTKSPFPFTKSNEDTLKQIFNKIKEIKEEKKINKHNQPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAR5</accession>
    <name>F6BAR5_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Molybdenum cofactor synthesis domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Metig_0323</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; molybdopterin biosynthesis.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95879.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798488.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0323"/>
    <dbReference type="EnsemblBacteria" id="AEF95879">
        <property type="protein sequence ID" value="AEF95879"/>
        <property type="gene ID" value="Metig_0323"/>
    </dbReference>
    <dbReference type="GeneID" id="10643159"/>
    <dbReference type="KEGG" id="mig:Metig_0323"/>
    <dbReference type="HOGENOM" id="CLU_010186_7_2_2"/>
    <dbReference type="OMA" id="KMPKVAF"/>
    <dbReference type="OrthoDB" id="51611at2157"/>
    <dbReference type="UniPathway" id="UPA00344"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006777">
        <property type="term" value="P:Mo-molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032324">
        <property type="term" value="P:molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00887">
        <property type="entry name" value="MoeA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.340.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.980.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036425">
        <property type="entry name" value="MoaB/Mog-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001453">
        <property type="entry name" value="MoaB/Mog_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038987">
        <property type="entry name" value="MoeA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005111">
        <property type="entry name" value="MoeA_C_domain_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036688">
        <property type="entry name" value="MoeA_C_domain_IV_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005110">
        <property type="entry name" value="MoeA_linker/N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036135">
        <property type="entry name" value="MoeA_linker/N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10192">
        <property type="entry name" value="PTHR10192"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00994">
        <property type="entry name" value="MoCF_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03454">
        <property type="entry name" value="MoeA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03453">
        <property type="entry name" value="MoeA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00852">
        <property type="entry name" value="MoCF_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53218">
        <property type="entry name" value="SSF53218"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63867">
        <property type="entry name" value="SSF63867"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63882">
        <property type="entry name" value="SSF63882"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00177">
        <property type="entry name" value="molyb_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0501">Molybdenum cofactor biosynthesis</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="276"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="domain" description="MoCF_biosynth" evidence="4">
        <location>
            <begin position="168"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEF95879.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="376" mass="42152" checksum="DDE0A0DC62281519" modified="2011-07-27" version="1">MISVREAEKILSKFYFNKTEYIKIEESYGRVLAEDIISNIDIPRFDRSDIDGYAVAEILEEYILIDEVFAGDDKDLEIKGNECVSVATGGKVPKNTKYVIPVENTIKIGNKIVVINFDRIEGISKKGCDVKKGDIILKKGTLINERNIGVLACLGIEEVKVYKTPKVGIISTGNELFKMTKDINSKTIASIVKKAGCEPIFLGIARDDPNEIKEHIIEASKKCDIIVTSGGVSIGKKDFLPRIVDELGEILFHKVKMRPGMPILFGKIDKKPIFCMPGNIVSCLVCSYVFLLPLLMKMAHKPYRRKIIKAKLSEKIISEPDFLYLWPVKLKNGIAYPTFKGSNMLTSILNADGLSVIKEHELKKDEGDMVDVWIFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-09-29" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAV9</accession>
    <name>F6BAV9_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1512</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97046.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF97046">
        <property type="protein sequence ID" value="AEF97046"/>
        <property type="gene ID" value="Metig_1512"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_1512"/>
    <dbReference type="HOGENOM" id="CLU_1444683_0_0_2"/>
    <dbReference type="OMA" id="ITIVYGI"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="38"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="64"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="96"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="134"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="160"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF97046.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="187" mass="22011" checksum="CAD666EC991C5507" modified="2011-07-27" version="1">MRTVLLFIIGLLFTNIKNVIFILALTILSLILSYFKRFYGLLLYSITLIYLVNQLVLENLFSVNGFYAIIFLILPSFLFLDTLIQRNIQFNEKYPIYLIPLISAILGFYNISTFLYTVLLFYTLYERFRYLDKVLIVKIFFLFITPIIALYIFYLLFLNIFNSGVSQCSVLISISGILLIIYKIKKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAZ8</accession>
    <name>F6BAZ8_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="3">CoA-disulfide reductase</fullName>
            <ecNumber evidence="3">1.8.1.14</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_1551</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.8.1.14" evidence="3"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97085.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799679.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1551"/>
    <dbReference type="EnsemblBacteria" id="AEF97085">
        <property type="protein sequence ID" value="AEF97085"/>
        <property type="gene ID" value="Metig_1551"/>
    </dbReference>
    <dbReference type="GeneID" id="10644424"/>
    <dbReference type="KEGG" id="mig:Metig_1551"/>
    <dbReference type="HOGENOM" id="CLU_003291_1_3_2"/>
    <dbReference type="OMA" id="ACGIPYW"/>
    <dbReference type="OrthoDB" id="40511at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050451">
        <property type="term" value="F:CoA-disulfide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045454">
        <property type="term" value="P:cell redox homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016156">
        <property type="entry name" value="FAD/NAD-linked_Rdtase_dimer_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004099">
        <property type="entry name" value="Pyr_nucl-diS_OxRdtase_dimer"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02852">
        <property type="entry name" value="Pyr_redox_dim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55424">
        <property type="entry name" value="SSF55424"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pyr_redox_2" evidence="2">
        <location>
            <begin position="2"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="domain" description="Pyr_redox_dim" evidence="1">
        <location>
            <begin position="329"/>
            <end position="425"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF97085.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="443" mass="48247" checksum="2175E2921A3E9309" modified="2011-07-27" version="1">MRTVIVGSGAGGLTTASTIRKYDKDMEIVVITMDRHVAYSPCAIPYVIEGEIKSFEDIIMHTPEDYKKERNIDIITKAKVVDVNSKENKVVYEKDGEKFELDYNYLVLATGGTPFVPPIEGKDLEGVFTVRTVEDGMKIDEYAKKSNSVVVAGAGAIGLEMAYAFKKRGLDVTVVEMAPQVLPRALDPDMAEKVKEYLEKEGIKIILNKPVEKIVGKDKVEGVVVDNKVIEADMVIMATGVRPNIELAKKAGCEIGKWAIKVDEKMQTSIPNIYAVGDCVEVVDFVTKQVTMSPFGTTAVRQGKVAAKNIVGIKAEFNPVLNAMVSKIGDLEIGGVGLSALSANQNGIPIVIGRCKALTRARYYPGGKPIEIKMIFDKDKRVVGCQIVGGERVAERIDAMSIAIFKGVKADELANMEFCYAPPVSMVNEPLALAAEDALEKFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>F6BB00</accession>
    <name>F6BB00_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Metig_1553</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97087.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1553"/>
    <dbReference type="EnsemblBacteria" id="AEF97087">
        <property type="protein sequence ID" value="AEF97087"/>
        <property type="gene ID" value="Metig_1553"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_1553"/>
    <dbReference type="HOGENOM" id="CLU_111441_1_0_2"/>
    <dbReference type="OMA" id="RETMICG"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005384">
        <property type="term" value="F:manganese ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030026">
        <property type="term" value="P:cellular manganese ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008217">
        <property type="entry name" value="Ccc1_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006682">
        <property type="entry name" value="CHP00267"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31851">
        <property type="entry name" value="PTHR31851"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01988">
        <property type="entry name" value="VIT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00267">
        <property type="entry name" value="TIGR00267"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="28"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="54"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="114"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="142"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="170"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="65"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEF97087.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="196" mass="21085" checksum="1FDB4B1A73B7CD18" modified="2011-07-27" version="1">MNNDDEQSPLSRVFKKFKFELNETKLRYVVRGIIDGSLSTLGVVIGASSGEPSLIISAGIGGGIANGLSNILGALTAEKASLEKEREMQERSLLKENGYLKKTIFYKQAVHETIICGFVDGISTMLGSMLPVVPFFVFEPRIAVFVAIAITLAILFALGMFIGKISRENLIVAGLKMVIGGVLVALAGFVIERIFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>F6BB08</accession>
    <name>F6BB08_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1561</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97095.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799689.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1561"/>
    <dbReference type="EnsemblBacteria" id="AEF97095">
        <property type="protein sequence ID" value="AEF97095"/>
        <property type="gene ID" value="Metig_1561"/>
    </dbReference>
    <dbReference type="GeneID" id="10644434"/>
    <dbReference type="KEGG" id="mig:Metig_1561"/>
    <dbReference type="HOGENOM" id="CLU_036466_0_0_2"/>
    <dbReference type="OMA" id="IYSINAN"/>
    <dbReference type="OrthoDB" id="20421at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="371"/>
            <end position="389"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF97095.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="400" mass="45879" checksum="0C20E2543AAACB3E" modified="2011-07-27" version="1">MLKKIFFILMIAIFVANTYALTIDNPQYRVDRSTPTDKYPNVIHPGDDVDVWFKITNDYDYNLKDITVTIKPYYPFEIKQVNPTKGVAKISHLNSGESDIVHFRLHVDENACSQDYKICVNVVATEYDKKEIINKINISKIYYLPVYGVANFEVFLDNYSITQSKLKDIKIIIKNMGTGIAKDTSVRLVGNENVDVVGPTQFYLGTIYPKGSVGIFTKIYAKNDIKDGVYPIYVSIFWIGEDGVLYNTTIPINIRVTIKPYNNSIFIYLDGCKYVDGKYEIDIGVANRGSIPLRHCVLTIKGLKLDRNVNYIGDLDEDDYEVVSFTTDKAINKTTEITTLLSYFDDYHNEYKVIKKLVVYPKKVEKSNNLLVYYIFGVIVVLILLYMVLKKIRKKEEFGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>F6BB12</accession>
    <name>F6BB12_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Helix-turn-helix domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1565</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97099.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799693.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1565"/>
    <dbReference type="EnsemblBacteria" id="AEF97099">
        <property type="protein sequence ID" value="AEF97099"/>
        <property type="gene ID" value="Metig_1565"/>
    </dbReference>
    <dbReference type="GeneID" id="10644438"/>
    <dbReference type="KEGG" id="mig:Metig_1565"/>
    <dbReference type="HOGENOM" id="CLU_085376_5_2_2"/>
    <dbReference type="OMA" id="ARESEPH"/>
    <dbReference type="OrthoDB" id="46214at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01381">
        <property type="entry name" value="HTH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00530">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="SSF51182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH cro/C1-type" evidence="1">
        <location>
            <begin position="17"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF97099.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="185" mass="20482" checksum="979949C7F8C7DDEB" modified="2011-07-27" version="1">MCDIMNNNISKIIGEKISKLRKEKRISLSKLAELAKISKSTLSSIESGNANPTISTLWAIADALNVSFGELLPKEFNEVDEFGITIKLIEQSEGKPKIEVYLMKLKPNSVREAKPHKKGVIEKVLVIKGKMVVGSKSSPKCLKVGEEIEFCGDVPHIYKALDEEVSAIVVVIYPPMEIVKIKSLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-06-02" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>F6BB13</accession>
    <name>F6BB13_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_1566</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97100.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799694.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF97100">
        <property type="protein sequence ID" value="AEF97100"/>
        <property type="gene ID" value="Metig_1566"/>
    </dbReference>
    <dbReference type="GeneID" id="10644439"/>
    <dbReference type="KEGG" id="mig:Metig_1566"/>
    <dbReference type="HOGENOM" id="CLU_105713_0_0_2"/>
    <dbReference type="OMA" id="GMLEDLC"/>
    <dbReference type="OrthoDB" id="133413at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF97100.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="228" mass="26594" checksum="C43049C1FA686FEF" modified="2011-07-27" version="1">MEKITKGKILIVEGEDEKRFFKEFLNFLGISDVQILKYDGKNNLKNRLKVWVRDSNFRNVNIFGIIIDADNNSHTSFQSVCDILRNVGLKPPQKMYVFSDGYPRIGVYIMPGNFENGMLEDLCIRTVKNCPLIQCVDNLIECAKNLYNNGSVKRFFESYEYFKQRGFYRNNFSNSNGIIRNIAKAKALAYLSIMPEIVYSVGVGAEKGYWNFESEELNELRRFLEYFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>F6BB28</accession>
    <name>F6BB28_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Carbon monoxide dehydrogenase</fullName>
            <ecNumber evidence="10">1.2.7.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">Metig_0357</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">CODH oxidizes carbon monoxide coupled, via CooF, to the reduction of a hydrogen cation by a hydrogenase (possibly CooH).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>CO + H2O + 2 oxidized [2Fe-2S]-[ferredoxin] = CO2 + 2 H(+) + 2 reduced [2Fe-2S]-[ferredoxin]</text>
            <dbReference type="Rhea" id="RHEA:21040"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:17245"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="EC" id="1.2.7.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>[Ni-Fe-S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:60400"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the Ni-containing carbon monoxide dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.2.7.4" evidence="10"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95913.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798522.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0357"/>
    <dbReference type="EnsemblBacteria" id="AEF95913">
        <property type="protein sequence ID" value="AEF95913"/>
        <property type="gene ID" value="Metig_0357"/>
    </dbReference>
    <dbReference type="GeneID" id="10643194"/>
    <dbReference type="KEGG" id="mig:Metig_0357"/>
    <dbReference type="HOGENOM" id="CLU_030631_0_0_2"/>
    <dbReference type="OMA" id="PEYMEQK"/>
    <dbReference type="OrthoDB" id="5135at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018492">
        <property type="term" value="F:carbon-monoxide dehydrogenase (acceptor) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043885">
        <property type="term" value="F:carbon-monoxide dehydrogenase (ferredoxin) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016151">
        <property type="term" value="F:nickel cation binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006091">
        <property type="term" value="P:generation of precursor metabolites and energy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01915">
        <property type="entry name" value="CODH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2030">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016101">
        <property type="entry name" value="CO_DH_a-bundle"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010047">
        <property type="entry name" value="CODH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004137">
        <property type="entry name" value="HCP/CODH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016099">
        <property type="entry name" value="Prismane-like_a/b-sand"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011254">
        <property type="entry name" value="Prismane-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30109">
        <property type="entry name" value="PTHR30109"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03063">
        <property type="entry name" value="Prismane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005023">
        <property type="entry name" value="CODH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56821">
        <property type="entry name" value="SSF56821"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01702">
        <property type="entry name" value="CO_DH_cata"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 11" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="8 10 11" id="KW-0408">Iron</keyword>
    <keyword evidence="9 10 11" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="7 10 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0533">Nickel</keyword>
    <keyword evidence="10 12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="11">
        <location>
            <position position="37"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="46"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="49"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="54"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="65"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="256"/>
        </location>
        <ligand>
            <name>[Ni-4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:47739"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="292"/>
        </location>
        <ligand>
            <name>[Ni-4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:47739"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="336"/>
        </location>
        <ligand>
            <name>[Ni-4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:47739"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="444"/>
        </location>
        <ligand>
            <name>[Ni-4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:47739"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="475"/>
        </location>
        <ligand>
            <name>[Ni-4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:47739"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="516"/>
        </location>
        <ligand>
            <name>[Ni-4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:47739"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00002452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010689"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR005023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR005023-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AEF95913.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="624" mass="67292" checksum="A0EFD94D3F699559" modified="2011-07-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>F6BB45</accession>
    <name>F6BB45_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Polyamine aminopropyltransferase</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">Putrescine aminopropyltransferase</fullName>
            <shortName evidence="2">PAPT</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="2">Spermidine synthase</fullName>
            <shortName evidence="2">SPDS</shortName>
            <shortName evidence="2">SPDSY</shortName>
            <ecNumber evidence="2">2.5.1.16</ecNumber>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2" type="primary">speE</name>
        <name evidence="7" type="ordered locus">Metig_0374</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the irreversible transfer of a propylamine group from the amino donor S-adenosylmethioninamine (decarboxy-AdoMet) to putrescine (1,4-diaminobutane) to yield spermidine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 5">
            <text>putrescine + S-adenosyl 3-(methylsulfanyl)propylamine = H(+) + S-methyl-5'-thioadenosine + spermidine</text>
            <dbReference type="Rhea" id="RHEA:12721"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17509"/>
            <dbReference type="ChEBI" id="CHEBI:57443"/>
            <dbReference type="ChEBI" id="CHEBI:57834"/>
            <dbReference type="ChEBI" id="CHEBI:326268"/>
            <dbReference type="EC" id="2.5.1.16"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amine and polyamine biosynthesis; spermidine biosynthesis; spermidine from putrescine: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer or homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the spermidine/spermine synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.16" evidence="2"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95930.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798539.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0374"/>
    <dbReference type="EnsemblBacteria" id="AEF95930">
        <property type="protein sequence ID" value="AEF95930"/>
        <property type="gene ID" value="Metig_0374"/>
    </dbReference>
    <dbReference type="GeneID" id="10643211"/>
    <dbReference type="KEGG" id="mig:Metig_0374"/>
    <dbReference type="HOGENOM" id="CLU_048199_0_0_2"/>
    <dbReference type="OMA" id="LWPGQSF"/>
    <dbReference type="OrthoDB" id="77175at2157"/>
    <dbReference type="UniPathway" id="UPA00248">
        <property type="reaction ID" value="UER00314"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004766">
        <property type="term" value="F:spermidine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008295">
        <property type="term" value="P:spermidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.140.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00198">
        <property type="entry name" value="Spermidine_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030374">
        <property type="entry name" value="PABS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030373">
        <property type="entry name" value="PABS_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001045">
        <property type="entry name" value="Spermi_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035246">
        <property type="entry name" value="Spermidine_synt_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037163">
        <property type="entry name" value="Spermidine_synt_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11558">
        <property type="entry name" value="PTHR11558"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17284">
        <property type="entry name" value="Spermine_synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00417">
        <property type="entry name" value="speE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01330">
        <property type="entry name" value="PABS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51006">
        <property type="entry name" value="PABS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0620">Polyamine biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0745">Spermidine biosynthesis</keyword>
    <keyword evidence="1 2 3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PABS" evidence="6">
        <location>
            <begin position="4"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="region of interest" description="S-methyl-5'-thioadenosine binding" evidence="2">
        <location>
            <begin position="139"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Spermidine binding" evidence="2">
        <location>
            <begin position="157"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="2 3">
        <location>
            <position position="157"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="33"/>
        </location>
        <ligand>
            <name>S-methyl-5'-thioadenosine</name>
            <dbReference type="ChEBI" id="CHEBI:17509"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="64"/>
        </location>
        <ligand>
            <name>spermidine</name>
            <dbReference type="ChEBI" id="CHEBI:57834"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="88"/>
        </location>
        <ligand>
            <name>spermidine</name>
            <dbReference type="ChEBI" id="CHEBI:57834"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="108"/>
        </location>
        <ligand>
            <name>S-methyl-5'-thioadenosine</name>
            <dbReference type="ChEBI" id="CHEBI:17509"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="164"/>
        </location>
        <ligand>
            <name>S-methyl-5'-thioadenosine</name>
            <dbReference type="ChEBI" id="CHEBI:17509"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEF95930.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="284" mass="32789" checksum="00DFDB450C2949B8" modified="2011-07-27" version="1">MKCQLWFSEYHTKNFALSLRVKDVLFTGKSKYQEIQILDTYDFGKVLILDNTFQTTERDEFIYHELISHPALFTHPNPKKVLVIGGGDGGTVREVVKHESVERVDFVELDEMVLELCKKYMPSLSCEMDNEKVNIIIGDGIKYVAECDEKYDVIIVDCPDPVGPAVGLFEKEFYKNVFKCLNDDGIMTQQTESPLYNLDLIKKIYTYLKDAGFSIVKPYVCSIPTYPSGFWCFTLASKKYDPLDVDENKIKEKLKNIPTKYYDEEVHKGVFLSSPKFLKDELKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2020-08-12" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>F6BB49</accession>
    <name>F6BB49_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_0378</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95934.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF95934">
        <property type="protein sequence ID" value="AEF95934"/>
        <property type="gene ID" value="Metig_0378"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0378"/>
    <dbReference type="HOGENOM" id="CLU_210692_0_0_2"/>
    <dbReference type="OMA" id="PSKEPFI"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF95934.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="58" mass="6731" checksum="FC2BF2683C812BDD" modified="2011-07-27" version="1">MATLLEEGVCVIKDEKTAKWVLEELTKPSKEPFISENVRVRTRKALKEGIEWAEKMQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>F6BB62</accession>
    <name>F6BB62_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 8 9">Chorismate synthase</fullName>
            <shortName evidence="8">CS</shortName>
            <ecNumber evidence="4 8 9">4.2.3.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">5-enolpyruvylshikimate-3-phosphate phospholyase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">aroC</name>
        <name evidence="10" type="ordered locus">Metig_0391</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the anti-1,4-elimination of the C-3 phosphate and the C-6 proR hydrogen from 5-enolpyruvylshikimate-3-phosphate (EPSP) to yield chorismate, which is the branch point compound that serves as the starting substrate for the three terminal pathways of aromatic amino acid biosynthesis. This reaction introduces a second double bond into the aromatic ring system.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8 9">
            <text>5-O-(1-carboxyvinyl)-3-phosphoshikimate = chorismate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:21020"/>
            <dbReference type="ChEBI" id="CHEBI:29748"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57701"/>
            <dbReference type="EC" id="4.2.3.5"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8 9">
            <name>FMNH2</name>
            <dbReference type="ChEBI" id="CHEBI:57618"/>
        </cofactor>
        <text evidence="8 9">Reduced FMN (FMNH(2)).</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 8 9">Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 7/7.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 8 9">Belongs to the chorismate synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="4.2.3.5" evidence="4 8 9"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95947.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798556.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0391"/>
    <dbReference type="EnsemblBacteria" id="AEF95947">
        <property type="protein sequence ID" value="AEF95947"/>
        <property type="gene ID" value="Metig_0391"/>
    </dbReference>
    <dbReference type="GeneID" id="10643228"/>
    <dbReference type="KEGG" id="mig:Metig_0391"/>
    <dbReference type="HOGENOM" id="CLU_034547_0_2_2"/>
    <dbReference type="OMA" id="MLSINAV"/>
    <dbReference type="OrthoDB" id="50229at2157"/>
    <dbReference type="UniPathway" id="UPA00053">
        <property type="reaction ID" value="UER00090"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004107">
        <property type="term" value="F:chorismate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009073">
        <property type="term" value="P:aromatic amino acid family biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:cellular amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009423">
        <property type="term" value="P:chorismate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd07304">
        <property type="entry name" value="Chorismate_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00300">
        <property type="entry name" value="Chorismate_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000453">
        <property type="entry name" value="Chorismate_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035904">
        <property type="entry name" value="Chorismate_synth_AroC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020541">
        <property type="entry name" value="Chorismate_synthase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21085">
        <property type="entry name" value="PTHR21085"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01264">
        <property type="entry name" value="Chorismate_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001456">
        <property type="entry name" value="Chorismate_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103263">
        <property type="entry name" value="SSF103263"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00033">
        <property type="entry name" value="aroC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00787">
        <property type="entry name" value="CHORISMATE_SYNTHASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00788">
        <property type="entry name" value="CHORISMATE_SYNTHASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8 9" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="6 8 9" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0274">FAD</keyword>
    <keyword evidence="8" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="8" id="KW-0288">FMN</keyword>
    <keyword evidence="7 8 9" id="KW-0456">Lyase</keyword>
    <keyword evidence="8" id="KW-0521">NADP</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="8">
        <location>
            <position position="46"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="123"/>
            <end position="125"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="253"/>
            <end position="254"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="298"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="313"/>
            <end position="317"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="340"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AEF95947.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="376" mass="41484" checksum="7F236BDE97B3E918" modified="2011-07-27" version="1">MNTIGDMFRVTTWGESHGKAVGAVVDGCPANLPLSEEDIQKELNRRRPGYSLFSTPRKEEDKVEILSGIFEGKTTGAPILALVYNKNQRPKDYSKIKDTPRPGHADLTYHLKYRHVDYRGGGRSSGRTTIGNVIGGAIAKKLLDYTHNIKIIGYAIKIGKIEGDFSYYKNEELFNNMDEDSLKNLIERIESNPLRCPSSNEKEMEEYVLNAIENQESVGGVVEIVALNVPIGVGNPIFNKLDGELAKSLMSINAVKGVEIGRGFESAEMFGSEMNDEIYFDDEKNIRLKTNNCGGILGGISCGTPIVIRIAIKPTPSISKKQHTIDLRTLENVDIAIEGRHDPIIVPRVIPVAESMVAITLADLMIKGGFIHPCRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>F6BB84</accession>
    <name>F6BB84_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Lysine 2,3-aminomutase YodO family protein</fullName>
            <ecNumber evidence="7">5.4.3.2</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Metig_0413</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <dbReference type="EC" id="5.4.3.2" evidence="7"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95969.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798578.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0413"/>
    <dbReference type="EnsemblBacteria" id="AEF95969">
        <property type="protein sequence ID" value="AEF95969"/>
        <property type="gene ID" value="Metig_0413"/>
    </dbReference>
    <dbReference type="GeneID" id="10643250"/>
    <dbReference type="KEGG" id="mig:Metig_0413"/>
    <dbReference type="HOGENOM" id="CLU_440512_0_0_2"/>
    <dbReference type="OMA" id="CQRMYDF"/>
    <dbReference type="OrthoDB" id="10285at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050066">
        <property type="term" value="F:lysine 2,3-aminomutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003739">
        <property type="entry name" value="Lys_aminomutase/Glu_NH3_mut"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30538">
        <property type="entry name" value="PTHR30538"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01070">
        <property type="entry name" value="PLP-dependent"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00238">
        <property type="entry name" value="TIGR00238"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="7" id="KW-0413">Isomerase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="6">
        <location>
            <begin position="297"/>
            <end position="511"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEF95969.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="598" mass="70531" checksum="7A177A9A46CDB59D" modified="2011-07-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBA7</accession>
    <name>F6BBA7_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="3">AAA ATPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_1581</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the archaeal ATPase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97114.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799707.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF97114">
        <property type="protein sequence ID" value="AEF97114"/>
        <property type="gene ID" value="Metig_1581"/>
    </dbReference>
    <dbReference type="GeneID" id="10644454"/>
    <dbReference type="KEGG" id="mig:Metig_1581"/>
    <dbReference type="HOGENOM" id="CLU_068608_0_0_2"/>
    <dbReference type="OrthoDB" id="50712at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011579">
        <property type="entry name" value="ATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01637">
        <property type="entry name" value="ATPase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA" evidence="2">
        <location>
            <begin position="20"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF97114.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="345" mass="41032" checksum="3DCE37AB7215332D" modified="2011-07-27" version="1">MFFNREKEIKEILSIIESEPNFIYFLYGPINSGKTALINEVINKLGKDYVVFYFDLREIFISKYDDFIEVLFEEYEEGNILELIKSLIKDSSVIYSFPIPKNTLEKLLDNKKPKNVFKYITNILLEIKNSGKKPIIILDELQKIGDLKINGYLIYELFNYFISLTKHKRLCHVFCLSSDSPFIEKIYSQAILDDRADYILIDDFDKEKAYEFMDFLSERILNRSLSKEEKELIYSYVGGKPVLIIKVMNRLKSDELENVLNYLLKDTRQRLKYFLEDVKEENKELYKEIIKALSLFKNNYEIEDIKINKRIRELLVKKNILFLDPIDGILKPQSFLIWKAIKEFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBE2</accession>
    <name>F6BBE2_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_1616</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97149.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799742.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1616"/>
    <dbReference type="EnsemblBacteria" id="AEF97149">
        <property type="protein sequence ID" value="AEF97149"/>
        <property type="gene ID" value="Metig_1616"/>
    </dbReference>
    <dbReference type="GeneID" id="10644489"/>
    <dbReference type="KEGG" id="mig:Metig_1616"/>
    <dbReference type="HOGENOM" id="CLU_2893256_0_0_2"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF97149.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="62" mass="7067" checksum="34787DB53A4D64AE" modified="2011-07-27" version="1">MNGDSILNIVGNFHGYVPLGDIVFYLQPFKKVASKWDALLTSFAMPLKNGYIGEIKWMKILY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBE9</accession>
    <name>F6BBE9_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">NADH ubiquinone oxidoreductase 20 kDa subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1623</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97156.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799749.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1623"/>
    <dbReference type="EnsemblBacteria" id="AEF97156">
        <property type="protein sequence ID" value="AEF97156"/>
        <property type="gene ID" value="Metig_1623"/>
    </dbReference>
    <dbReference type="GeneID" id="10644496"/>
    <dbReference type="KEGG" id="mig:Metig_1623"/>
    <dbReference type="HOGENOM" id="CLU_055737_7_3_2"/>
    <dbReference type="OMA" id="QYGIYVH"/>
    <dbReference type="OrthoDB" id="100307at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008137">
        <property type="term" value="F:NADH dehydrogenase (ubiquinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048038">
        <property type="term" value="F:quinone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006137">
        <property type="entry name" value="NADH_UbQ_OxRdtase-like_20kDa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006138">
        <property type="entry name" value="NADH_UQ_OxRdtase_20Kd_su"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01058">
        <property type="entry name" value="Oxidored_q6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01150">
        <property type="entry name" value="COMPLEX1_20K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0830">Ubiquinone</keyword>
    <feature type="domain" description="Oxidored_q6" evidence="1">
        <location>
            <begin position="21"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF97156.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="154" mass="16854" checksum="105FC6668E7A4479" modified="2011-07-27" version="1">MLKEFVRKRSIHVCVVNTGGCNGCDIEIVSCLAPRYDIEQYGIFVHNNPREADVLLVTGPVTLPWKERLKEIYEKTPEPKIVVAVGACALSGGIFKEGHVVGGIDKVIPVDAKIPGCPPRPSEIIEAILKVGPDALAMKEKMIKEKIRKGEVII</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBJ6</accession>
    <name>F6BBJ6_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="6">ABC-type transporter, integral membrane subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Metig_0449</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96005.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798614.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0449"/>
    <dbReference type="EnsemblBacteria" id="AEF96005">
        <property type="protein sequence ID" value="AEF96005"/>
        <property type="gene ID" value="Metig_0449"/>
    </dbReference>
    <dbReference type="GeneID" id="10643286"/>
    <dbReference type="KEGG" id="mig:Metig_0449"/>
    <dbReference type="HOGENOM" id="CLU_016047_14_1_2"/>
    <dbReference type="OMA" id="NEYNFVA"/>
    <dbReference type="OrthoDB" id="97465at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="7"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="48"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="84"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="118"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="176"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="225"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="5">
        <location>
            <begin position="46"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEF96005.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="250" mass="27905" checksum="DED5DCFD31276E75" modified="2011-07-27" version="1">MRIFYISLFIISLMLVLFIILPVINMLLNPGDVKGALMDKEVIDSLLVSLYAASTATIIALFFGIPLGYLLARYDFRGKDFVEAIVDLPMAIPHSVVGIMILSFFYNNPIGIFNNFGAYIVDNFWGIVVVMLFVGVPFMVNSARDGFLMVDEELEHVSRTLGASQMRTFFNISLPLIYNNIISGSILTFARGISEVGAILIVAYFPKTMPVLILERFNNFGLSASKPIAVIMILVSIMIFALLRLVRKRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-06-02" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBK5</accession>
    <name>F6BBK5_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0458</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96014.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96014">
        <property type="protein sequence ID" value="AEF96014"/>
        <property type="gene ID" value="Metig_0458"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0458"/>
    <dbReference type="HOGENOM" id="CLU_2766084_0_0_2"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="23"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96014.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="74" mass="8074" checksum="72146C835731090C" modified="2011-07-27" version="1">MMPLMKVDTGDLRRRTLLSVKPMGGAGVPVTGMETDDMFPMNPEGTEADDIQVQRGHLSMIVHVGQTPLLRLAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBP9</accession>
    <name>F6BBP9_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_0502</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96058.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798666.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0502"/>
    <dbReference type="EnsemblBacteria" id="AEF96058">
        <property type="protein sequence ID" value="AEF96058"/>
        <property type="gene ID" value="Metig_0502"/>
    </dbReference>
    <dbReference type="GeneID" id="10643339"/>
    <dbReference type="KEGG" id="mig:Metig_0502"/>
    <dbReference type="HOGENOM" id="CLU_130794_0_0_2"/>
    <dbReference type="OMA" id="KFYFGDI"/>
    <dbReference type="OrthoDB" id="101586at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF96058.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="151" mass="17527" checksum="B72DFBED46A0AE68" modified="2011-07-27" version="1">MKRILFFTFLTVFLISFAILGHQKDDNEIIKEKLLEFGYPAKGYVIINNTIRYSDGSFVVLSTPPKKYPITAIGAYNLAKKYLDEKYNKMLEEHNYYLDVEPGSIREYEKDGNYYWQFKLLFGKKGTKGDFMGYVLVNRKTGSVKIKGLFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBQ4</accession>
    <name>F6BBQ4_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0507</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96063.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798671.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0507"/>
    <dbReference type="EnsemblBacteria" id="AEF96063">
        <property type="protein sequence ID" value="AEF96063"/>
        <property type="gene ID" value="Metig_0507"/>
    </dbReference>
    <dbReference type="GeneID" id="10643344"/>
    <dbReference type="KEGG" id="mig:Metig_0507"/>
    <dbReference type="HOGENOM" id="CLU_1736447_0_0_2"/>
    <dbReference type="OMA" id="STHTICI"/>
    <dbReference type="OrthoDB" id="116343at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="19"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="119"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96063.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="159" mass="18776" checksum="FE0B2A34066B110A" modified="2011-07-27" version="1">MGFLSIFKRDRQENIQNLQQTEIREINDYETKYNELLEEINMLKNDLLAMFRKDAYYLSLSKIAYTGGVEEAEIWIDYHSGRISALTAPLTRLFRIIGEDPSILEQILLEEKNKAINDILSCEKIQEALEEDIKQLREAKDFKEKLEQFKKLIEEGKIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBQ8</accession>
    <name>F6BBQ8_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">UDP-N-acetyl-D-mannosamine dehydrogenase</fullName>
            <ecNumber evidence="3">1.1.1.336</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">UDP-ManNAc 6-dehydrogenase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Metig_1656</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>H2O + 2 NAD(+) + UDP-N-acetyl-alpha-D-mannosamine = 3 H(+) + 2 NADH + UDP-N-acetyl-alpha-D-mannosaminouronate</text>
            <dbReference type="Rhea" id="RHEA:25780"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:68623"/>
            <dbReference type="ChEBI" id="CHEBI:70731"/>
            <dbReference type="EC" id="1.1.1.336"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the UDP-glucose/GDP-mannose dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.336" evidence="3"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97188.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799779.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1656"/>
    <dbReference type="EnsemblBacteria" id="AEF97188">
        <property type="protein sequence ID" value="AEF97188"/>
        <property type="gene ID" value="Metig_1656"/>
    </dbReference>
    <dbReference type="GeneID" id="10644529"/>
    <dbReference type="KEGG" id="mig:Metig_1656"/>
    <dbReference type="HOGENOM" id="CLU_023810_3_2_2"/>
    <dbReference type="OMA" id="GHCLPID"/>
    <dbReference type="OrthoDB" id="41144at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016628">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-CH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0089714">
        <property type="term" value="F:UDP-N-acetyl-D-mannosamine dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000271">
        <property type="term" value="P:polysaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017476">
        <property type="entry name" value="UDP-Glc/GDP-Man"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014027">
        <property type="entry name" value="UDP-Glc/GDP-Man_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036220">
        <property type="entry name" value="UDP-Glc/GDP-Man_DH_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014026">
        <property type="entry name" value="UDP-Glc/GDP-Man_DH_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001732">
        <property type="entry name" value="UDP-Glc/GDP-Man_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028359">
        <property type="entry name" value="UDP_ManNAc/GlcNAc_DH"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00984">
        <property type="entry name" value="UDPG_MGDP_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03720">
        <property type="entry name" value="UDPG_MGDP_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03721">
        <property type="entry name" value="UDPG_MGDP_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF500136">
        <property type="entry name" value="UDP_ManNAc_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000124">
        <property type="entry name" value="UDPglc_GDPman_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00984">
        <property type="entry name" value="UDPG_MGDP_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="SSF48179"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52413">
        <property type="entry name" value="SSF52413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03026">
        <property type="entry name" value="NDP-sugDHase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0520">NAD</keyword>
    <keyword evidence="5 10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UDPG_MGDP_dh_C" evidence="9">
        <location>
            <begin position="318"/>
            <end position="415"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00016796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00030172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR000124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AEF97188.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="439" mass="48990" checksum="23E2AA8F9E9B779D" modified="2011-07-27" version="1">MEVINVEDEVSKEKTKKNKKICVVGLGYIGLPTASMLANHGYKVVGVDIDENKVNAIKNGTLQIEEPGLMTLVKGAINSGNLIVKTEPEEADIFIICVPTPAVENNGTKKCDLSYVWSAARSIKPYLKKGNLVIIESTIPPNTTNKLHEFFGEGIYVAHCPERVLPGRILIELVENDRIIGGVDKKSAELAKEIYQSFVDGNIYLTDATTAEMVKLMENTFRDVNIALANEFAKVCEEIGVNVWEAIELANKHPRVNILKPGPGVGGHCISIDPWFIVEKSKNAKLIKTARELNDKMPEYVCNLILREVEGIENPKIAILGVTYKGNVDDTRESPAKKIVAMLISKNFTVKCYDPHAKVFDYELCDLRSCIENADCIVVLADHNEFKDFNEEEIKNIGSKMRTKKIIDTKNILNHELWEKCGFDVVLLGDGKRFRKSKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBS8</accession>
    <name>F6BBS8_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1676</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97208.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1676"/>
    <dbReference type="EnsemblBacteria" id="AEF97208">
        <property type="protein sequence ID" value="AEF97208"/>
        <property type="gene ID" value="Metig_1676"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_1676"/>
    <dbReference type="HOGENOM" id="CLU_449523_0_0_2"/>
    <dbReference type="OMA" id="YGRPERY"/>
    <dbReference type="OrthoDB" id="18620at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="196"/>
            <end position="325"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF97208.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="574" mass="68706" checksum="0382A9BBACCE148D" modified="2011-07-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-06-02" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBU4</accession>
    <name>F6BBU4_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_1692</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97224.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799814.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF97224">
        <property type="protein sequence ID" value="AEF97224"/>
        <property type="gene ID" value="Metig_1692"/>
    </dbReference>
    <dbReference type="GeneID" id="10644565"/>
    <dbReference type="KEGG" id="mig:Metig_1692"/>
    <dbReference type="HOGENOM" id="CLU_1850644_0_0_2"/>
    <dbReference type="OMA" id="DYIFLFT"/>
    <dbReference type="OrthoDB" id="96760at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF97224.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="138" mass="16081" checksum="A9AC44AF70E2F430" modified="2011-07-27" version="1">MENSNDNQIIEMPIPPGLPQSIIARLLEMCNVKFDLRKDEIHDVKYPVLCGTLEDLKIAKEYLELVTETKLALREIARLARKFKTKVKLYTNDDEYRYILEIIANDIANKDKIEIVEEEPEGECEVIKVLDKEVKVYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBV4</accession>
    <name>F6BBV4_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Probable tRNA sulfurtransferase</fullName>
            <ecNumber evidence="6">2.8.1.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Sulfur carrier protein ThiS sulfurtransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Thiamine biosynthesis protein ThiI</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">tRNA 4-thiouridine synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">thiI</name>
        <name evidence="9" type="ordered locus">Metig_1702</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. This is a step in the synthesis of thiazole, in the thiamine biosynthesis pathway. The sulfur is donated as persulfide by IscS.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>[ThiI sulfur-carrier protein]-S-sulfanyl-L-cysteine + a uridine in tRNA + ATP + H(+) + 2 reduced [2Fe-2S]-[ferredoxin] = [ThiI sulfur-carrier protein]-L-cysteine + a 4-thiouridine in tRNA + AMP + diphosphate + 2 oxidized [2Fe-2S]-[ferredoxin]</text>
            <dbReference type="Rhea" id="RHEA:24176"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="Rhea" id="RHEA-COMP:13337"/>
            <dbReference type="Rhea" id="RHEA-COMP:13338"/>
            <dbReference type="Rhea" id="RHEA-COMP:13339"/>
            <dbReference type="Rhea" id="RHEA-COMP:13340"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:61963"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:136798"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.8.1.4"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>[sulfur-carrier protein ThiS]-C-terminal Gly-Gly-AMP + AH2 + S-sulfanyl-L-cysteinyl-[cysteine desulfurase] = [sulfur-carrier protein ThiS]-C-terminal Gly-NH-CH2-C(O)SH + A + AMP + H(+) + L-cysteinyl-[cysteine desulfurase]</text>
            <dbReference type="Rhea" id="RHEA:43340"/>
            <dbReference type="Rhea" id="RHEA-COMP:12157"/>
            <dbReference type="Rhea" id="RHEA-COMP:12158"/>
            <dbReference type="Rhea" id="RHEA-COMP:12908"/>
            <dbReference type="Rhea" id="RHEA-COMP:12910"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:61963"/>
            <dbReference type="ChEBI" id="CHEBI:90618"/>
            <dbReference type="ChEBI" id="CHEBI:90619"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="6">Cofactor biosynthesis; thiamine diphosphate biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the ThiI family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.8.1.4" evidence="6"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97234.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799824.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1702"/>
    <dbReference type="EnsemblBacteria" id="AEF97234">
        <property type="protein sequence ID" value="AEF97234"/>
        <property type="gene ID" value="Metig_1702"/>
    </dbReference>
    <dbReference type="GeneID" id="10644575"/>
    <dbReference type="KEGG" id="mig:Metig_1702"/>
    <dbReference type="HOGENOM" id="CLU_037952_4_0_2"/>
    <dbReference type="OMA" id="SMPEFCG"/>
    <dbReference type="OrthoDB" id="57901at2157"/>
    <dbReference type="UniPathway" id="UPA00060"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004810">
        <property type="term" value="F:tRNA adenylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140741">
        <property type="term" value="F:tRNA U4 sulfurtransferase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009228">
        <property type="term" value="P:thiamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009229">
        <property type="term" value="P:thiamine diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034227">
        <property type="term" value="P:tRNA thio-modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01712">
        <property type="entry name" value="ThiI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00021">
        <property type="entry name" value="ThiI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020536">
        <property type="entry name" value="ThiI_AANH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004114">
        <property type="entry name" value="THUMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003720">
        <property type="entry name" value="tRNA_STrfase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02568">
        <property type="entry name" value="ThiI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02926">
        <property type="entry name" value="THUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00981">
        <property type="entry name" value="THUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00342">
        <property type="entry name" value="TIGR00342"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51165">
        <property type="entry name" value="THUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5 6" id="KW-0784">Thiamine biosynthesis</keyword>
    <keyword evidence="2 6 9" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="THUMP" evidence="8">
        <location>
            <begin position="60"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="186"/>
            <end position="187"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="268"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="290"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="299"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AEF97234.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="375" mass="42607" checksum="BE01C450F23423A8" modified="2011-07-27" version="1">MEKIIVRYGEIGTKSKQTRKRFEELLAKSIRKLLQKYDIHPDVSILHTRLLVEVNKEDFEKTLELLRKVPGISSFSPCVECKLDIDNIVKTAKRVVKDKLKSIDKDEITFAVKTQRVQKYFPLTSPEINSIVGEKIIEEFGLKVDLKNPDILVEIEVLKDRAFIFTERIRGIGGLPVGTQGNVLVLMSDGIDSPVAAYLMIRRGCNATLLHLKLSEEGLKKTRKLAGILSDYDCDLRFVVKDFRDELKSIKENLKKLDKEEYTCIFCKRTMLKIAEKYAKDLGCDAIVTGDNLGQVASQTLKNLRAISEGIELPILRPLIGMDKNEIIEIAKEIGTFEISTSKEIKCFAVPKYPVTKANIEKIKEIEKELKIRGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBX0</accession>
    <name>F6BBX0_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Small GTP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1718</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97250.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799839.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1718"/>
    <dbReference type="EnsemblBacteria" id="AEF97250">
        <property type="protein sequence ID" value="AEF97250"/>
        <property type="gene ID" value="Metig_1718"/>
    </dbReference>
    <dbReference type="GeneID" id="10644592"/>
    <dbReference type="KEGG" id="mig:Metig_1718"/>
    <dbReference type="HOGENOM" id="CLU_1472124_0_0_2"/>
    <dbReference type="OMA" id="VGGTTKM"/>
    <dbReference type="OrthoDB" id="79086at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="G" evidence="1">
        <location>
            <begin position="7"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF97250.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="186" mass="21218" checksum="A8A408410EBEC188" modified="2011-07-27" version="1">MENKIFKIAILGPENAGKSSIMNALFGKYVSLVSEVGGTTKMPVKRYWGKLKVGREKKQPKFVDVVFVDLGGLYVREKQSPIMVGSVLEQTYKEIDDADMIIHVIDGKEGLSKSFERLHHLLKFRYRKPIIVVINKCDLINGDERRQLKNYVEYRLNNRALLVSALTREGIDDLMEAILKYIKEGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>F6BC44</accession>
    <name>F6BC44_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="7">ABC-type transporter, integral membrane subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Metig_0571</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96125.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798732.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0571"/>
    <dbReference type="EnsemblBacteria" id="AEF96125">
        <property type="protein sequence ID" value="AEF96125"/>
        <property type="gene ID" value="Metig_0571"/>
    </dbReference>
    <dbReference type="GeneID" id="10643409"/>
    <dbReference type="KEGG" id="mig:Metig_0571"/>
    <dbReference type="HOGENOM" id="CLU_039929_1_0_2"/>
    <dbReference type="OMA" id="NMGWFLI"/>
    <dbReference type="OrthoDB" id="74197at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001851">
        <property type="entry name" value="ABC_transp_permease"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02653">
        <property type="entry name" value="BPD_transp_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="59"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="92"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="142"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="191"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="221"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="276"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEF96125.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="304" mass="32299" checksum="79C58786A7784744" modified="2011-07-27" version="1">MAVVEGAITYANLMVLLSLGLTLTYITTGVPNFAQGSFAVVGAYFALMLFRLAGIHPYYAIPLAFIIGGIVGIVTYACILKPLIKKNASVEMLMIATLAWDLILFGVVGAFAETLGSITKKTETQFIFTNLDFDVFGLSGRLVISSLLIVLTLLGLYMLLYKTKFGIALRASMENPSLAEAMGVNVENTRLFSWFLSGALAGMAGAVLPFMQVIVPATGGFIIVSIFAASIVGGLQHIAGALIGGYIVGISESLVTYGLSSIFGTGVLVYSKVVSLVMMIFTLLFAPGGITGTKLWEKLTKLWD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>F6BC66</accession>
    <name>F6BC66_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Oxidoreductase/nitrogenase component 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_1740</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97272.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799861.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1740"/>
    <dbReference type="EnsemblBacteria" id="AEF97272">
        <property type="protein sequence ID" value="AEF97272"/>
        <property type="gene ID" value="Metig_1740"/>
    </dbReference>
    <dbReference type="GeneID" id="10644614"/>
    <dbReference type="KEGG" id="mig:Metig_1740"/>
    <dbReference type="HOGENOM" id="CLU_025876_2_0_2"/>
    <dbReference type="OMA" id="GLNNMID"/>
    <dbReference type="OrthoDB" id="15283at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016163">
        <property type="term" value="F:nitrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009399">
        <property type="term" value="P:nitrogen fixation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000510">
        <property type="entry name" value="Nase/OxRdtase_comp1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000318">
        <property type="entry name" value="Nase_comp1_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00148">
        <property type="entry name" value="Oxidored_nitro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00090">
        <property type="entry name" value="NITROGENASE_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0535">Nitrogen fixation</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Oxidored_nitro" evidence="2">
        <location>
            <begin position="20"/>
            <end position="450"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF97272.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="462" mass="51711" checksum="2AA22558EC658952" modified="2011-07-27" version="1">MGICYVEKQRAGTINPNKTCQPIGAMWATLGVHRGIPFVQGSQGCCTYVRYTFNRHFREPVSIAVASFHEDAAVYGGMNNLVEGLRNLVARYDPDLISVVTTCSSETIGDDVEAFIRAARKKIAKELGEEKAQLPIIPVHCPSYQASHVKGYDNASKAFMEYLAKKDEEKEPHKINIIPGFGVNPGDILEIKRILDMFGLKEGEDYSILFDISETLYQPLREPIKEIPYYPKGGTKVEEFVDAANAKATFALCRHAGGAGALYLQRRFKVPAFYGLPIGLKNTDDFIINIARVTGLEIPDKLLDERGKLVDAIVDTIHYTMDKKVGIFGDPDFVIAVSRFCCEIGMKPVVVNTQTPSKTYKKSMEEIANEHNVDIEVQYSDLWDFEKSVKAKGVDLLIGHPRGGVKIAEDMGIGLVRMGFPIYDRVGYFRWPIVGYMGSLRFFDEIVNTILDTQVPWDQKQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCB8</accession>
    <name>F6BCB8_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Coenzyme F420 hydrogenase, subunit beta</fullName>
            <ecNumber evidence="5">1.12.98.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Metig_1792</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.12.98.1" evidence="5"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97324.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799913.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="SMR" id="F6BCB8"/>
    <dbReference type="STRING" id="880724.Metig_1792"/>
    <dbReference type="EnsemblBacteria" id="AEF97324">
        <property type="protein sequence ID" value="AEF97324"/>
        <property type="gene ID" value="Metig_1792"/>
    </dbReference>
    <dbReference type="GeneID" id="10644669"/>
    <dbReference type="KEGG" id="mig:Metig_1792"/>
    <dbReference type="HOGENOM" id="CLU_037958_0_0_2"/>
    <dbReference type="OMA" id="REYGCEK"/>
    <dbReference type="OrthoDB" id="49654at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050454">
        <property type="term" value="F:coenzyme F420 hydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016151">
        <property type="term" value="F:nickel cation binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007516">
        <property type="entry name" value="Co_F420_Hydgase/DH_bsu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045220">
        <property type="entry name" value="FRHB/FDHB/HCAR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017679">
        <property type="entry name" value="FrhB_archaea"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007525">
        <property type="entry name" value="FrhB_FdhB_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31332">
        <property type="entry name" value="PTHR31332"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04432">
        <property type="entry name" value="FrhB_FdhB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04422">
        <property type="entry name" value="FrhB_FdhB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03289">
        <property type="entry name" value="frhB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0408">Iron</keyword>
    <keyword evidence="2" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FrhB_FdhB_N" evidence="3">
        <location>
            <begin position="10"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="domain" description="FrhB_FdhB_C" evidence="4">
        <location>
            <begin position="95"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF04432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEF97324.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="282" mass="31166" checksum="B3B6CFF9D33E5B3A" modified="2011-07-27" version="1">MDAFGKYKTVVSARATDKKILKKSQDGGIVSAAFIYGLENGLLDGVIVADNAGEFKAVPKVATTPEEVLEAAGTKYTVCPNISVLKSAVREYALEKVGIVGTPCQIRAVRKLMKYPVGFRHTDSKIALTIGIFCMENFPYMGLKTIVEEHCGVKMEDVVKMDIGKGKFWVYTKWGETKAIKLKETHPYEQIACHVCTDYTAELADISTGSVGSPDGWSTVFVRTTKGEEFFNKMVEDGYLEIKPIEEVKPGLGLVEKLALTKKEKNTKEIEHRKELGLPVPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCE6</accession>
    <name>F6BCE6_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Alpha-L-glutamate ligase, RimK family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_0603</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96157.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798763.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0603"/>
    <dbReference type="EnsemblBacteria" id="AEF96157">
        <property type="protein sequence ID" value="AEF96157"/>
        <property type="gene ID" value="Metig_0603"/>
    </dbReference>
    <dbReference type="GeneID" id="10643441"/>
    <dbReference type="KEGG" id="mig:Metig_0603"/>
    <dbReference type="HOGENOM" id="CLU_054353_2_0_2"/>
    <dbReference type="OMA" id="CYMNIAS"/>
    <dbReference type="OrthoDB" id="42812at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013651">
        <property type="entry name" value="ATP-grasp_RimK-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004666">
        <property type="entry name" value="RpS6_RimK/Lys_biosynth_LsyX"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08443">
        <property type="entry name" value="RimK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00768">
        <property type="entry name" value="rimK_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-grasp" evidence="2">
        <location>
            <begin position="103"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF96157.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="291" mass="33126" checksum="F869A9DC7F7E507F" modified="2011-07-27" version="1">MKMGIISEEKDWVVNELKKSMEKNGIKPEIIKPSNIISGIGMGFKLKCNDKNLLDLDCAFVRNIGNEVEMFHRFDFLKYLEHYIPLINPLDGIENAGNKYRCSFLMDLKNIPQPRTVVTENIDEALKWVNAFEDCVVKPLFGHQGKGIMRINGGSLISKLNKLTEFKKKYGAFYIQEFVKNPNNVYRDIRAFVVGDEVVSAMYRVSDNWITNIHQNAKPEECEVNDEIEELAIKAKDALGLVYAGVDLIESRDGLKVLEVNATPSWEGLSKISKVNIADRIIEEVVNLLKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCG9</accession>
    <name>F6BCG9_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 6">V-type ATP synthase subunit D</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5 6">V-ATPase subunit D</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">atpD</name>
        <name evidence="7" type="ordered locus">Metig_0630</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Produces ATP from ADP in the presence of a proton gradient across the membrane.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the V-ATPase D subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96180.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798785.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0630"/>
    <dbReference type="EnsemblBacteria" id="AEF96180">
        <property type="protein sequence ID" value="AEF96180"/>
        <property type="gene ID" value="Metig_0630"/>
    </dbReference>
    <dbReference type="GeneID" id="10643469"/>
    <dbReference type="KEGG" id="mig:Metig_0630"/>
    <dbReference type="HOGENOM" id="CLU_069688_2_1_2"/>
    <dbReference type="OMA" id="TQKRANA"/>
    <dbReference type="OrthoDB" id="93236at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042777">
        <property type="term" value="P:proton motive force-driven plasma membrane ATP synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00271">
        <property type="entry name" value="ATP_synth_D_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002699">
        <property type="entry name" value="V_ATPase_D"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11671">
        <property type="entry name" value="PTHR11671"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01813">
        <property type="entry name" value="ATP-synt_D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00309">
        <property type="entry name" value="V_ATPase_subD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0066">ATP synthesis</keyword>
    <keyword evidence="6" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="4 6" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00020748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00031705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEF96180.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="216" mass="24994" checksum="5359C0D26AD31A21" modified="2011-07-27" version="1">MQQVNPTRMELLKLKNKIKLAQKGHKLLKQKRDALIMEFFDIIEQASGIREKVNKCLEKAYQDLIMAQAVMGILSVKEASFAAKNDKINLDVDTRNIMGVTVPVFEIENVRRNVSERGYSIYNTCAKLDEAAKAFEEALELIVELAEIETSIKLLAEEIITTKRRVNALEYVIIPKLEETKKYISMRLDEMERENFFRLKLIKSRIERRESGEKVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCH2</accession>
    <name>F6BCH2_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Alpha-L-glutamate ligase, RimK family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_0633</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96183.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798788.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0633"/>
    <dbReference type="EnsemblBacteria" id="AEF96183">
        <property type="protein sequence ID" value="AEF96183"/>
        <property type="gene ID" value="Metig_0633"/>
    </dbReference>
    <dbReference type="GeneID" id="10643472"/>
    <dbReference type="KEGG" id="mig:Metig_0633"/>
    <dbReference type="HOGENOM" id="CLU_054353_2_1_2"/>
    <dbReference type="OMA" id="VDKSMTS"/>
    <dbReference type="OrthoDB" id="42812at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013651">
        <property type="entry name" value="ATP-grasp_RimK-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004666">
        <property type="entry name" value="RpS6_RimK/Lys_biosynth_LsyX"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08443">
        <property type="entry name" value="RimK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="SSF52440"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00768">
        <property type="entry name" value="rimK_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-grasp" evidence="2">
        <location>
            <begin position="89"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF96183.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="273" mass="31906" checksum="2DC944EFD812584B" modified="2011-07-27" version="1">MIGLLAHKKTLENELILEAFKKRNLKVDFVDSREIINGIENYHYDLILSRVERDYLFEGIYALKFFESEGLNVINNSETVYTCQNKYLTYLKLKKYMPKSFLTYTNNFEKILNAMEKNKMDFPIVIKPIYGGYGNGVVKVNSREELKNIVELLKFHNREIFIQEFVKYENDVRAFVVGDDIIGVMERIPKNDWRANFSLGADVREFKLDNETEELILKCVEKVGADIVGVDVLVCRDKSYILEMNITPQFRGMMKFVDVPNAIVDFCISRIKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-06-02" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCI8</accession>
    <name>F6BCI8_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transposase IS4 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0649</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96199.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798802.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96199">
        <property type="protein sequence ID" value="AEF96199"/>
        <property type="gene ID" value="Metig_0649"/>
    </dbReference>
    <dbReference type="GeneID" id="10643489"/>
    <dbReference type="KEGG" id="mig:Metig_0649"/>
    <dbReference type="HOGENOM" id="CLU_071602_0_0_2"/>
    <dbReference type="OMA" id="GDSWYSS"/>
    <dbReference type="OrthoDB" id="42868at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:transposition, DNA-mediated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002559">
        <property type="entry name" value="Transposase_11"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01609">
        <property type="entry name" value="DDE_Tnp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DDE_Tnp_1" evidence="1">
        <location>
            <begin position="97"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96199.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="332" mass="39092" checksum="6AABB541C1B131EE" modified="2011-07-27" version="1">MLKKMGSIVYMLFILLSEVNSCVELSKTLRIFGINISHDTINRILWDEGISPQERLFSYVKDFITSDYNIIVIDDFVIDKIYSKSTEFTYYCWSNLHKRVVKGIHIVDCIVTNGKNIIPIDFRVYDKQRDGKTKNDLCREIISSLVEKGLNIRYICFDSWYSSKENLKLIDKFGLFYLCRIKRNRKIKLSKNGEWISIKELGEIPKSGLIVYLRKVGYVKLFCLSKNGKAVYYITNNLFMDFEEFQEVRNASWRIEEFHRGVKQCCNIGNFFVRKRLPILGHISLAMRAFFILERIRIDKKITWYEFRRELNRIAVGNAIISLCKEAGLLLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-09-29" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCK6</accession>
    <name>F6BCK6_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_0667</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96217.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798820.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96217">
        <property type="protein sequence ID" value="AEF96217"/>
        <property type="gene ID" value="Metig_0667"/>
    </dbReference>
    <dbReference type="GeneID" id="10643507"/>
    <dbReference type="KEGG" id="mig:Metig_0667"/>
    <dbReference type="HOGENOM" id="CLU_2565514_0_0_2"/>
    <dbReference type="OMA" id="EVICVEA"/>
    <dbReference type="OrthoDB" id="125376at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF96217.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="94" mass="11039" checksum="396BC98BF9F6BEE9" modified="2011-07-27" version="1">MNKRKFLFLTFEGFTYSQKESIAPDVENIQVIGYSEGKDEYEAFNNFVLRNSWILDTGFNEIICIEVKQAISEGKRFYLEEFRSLSSQKLHTPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCK9</accession>
    <name>F6BCK9_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 5">50S ribosomal protein L15e</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">rpl15e</name>
        <name evidence="7" type="ordered locus">Metig_0670</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the eukaryotic ribosomal protein eL15 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96220.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798823.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0670"/>
    <dbReference type="EnsemblBacteria" id="AEF96220">
        <property type="protein sequence ID" value="AEF96220"/>
        <property type="gene ID" value="Metig_0670"/>
    </dbReference>
    <dbReference type="GeneID" id="10643510"/>
    <dbReference type="KEGG" id="mig:Metig_0670"/>
    <dbReference type="HOGENOM" id="CLU_080796_0_0_2"/>
    <dbReference type="OMA" id="KYMQELY"/>
    <dbReference type="OrthoDB" id="85023at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1120.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00256">
        <property type="entry name" value="Ribosomal_L15e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024794">
        <property type="entry name" value="Rbsml_L15e_core_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000439">
        <property type="entry name" value="Ribosomal_L15e"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020926">
        <property type="entry name" value="Ribosomal_L15e_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020925">
        <property type="entry name" value="Ribosomal_L15e_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012678">
        <property type="entry name" value="Ribosomal_L23/L15e_core_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11847">
        <property type="entry name" value="PTHR11847"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00827">
        <property type="entry name" value="Ribosomal_L15e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01384">
        <property type="entry name" value="Ribosomal_L15e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54189">
        <property type="entry name" value="SSF54189"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01194">
        <property type="entry name" value="RIBOSOMAL_L15E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 5 7" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="165"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00029524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEF96220.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="194" mass="22466" checksum="0DD4F3D62FC6EE78" modified="2011-07-27" version="1">MGIYKYIRDAWKRPKDSYVKQLLWERMQQWRREPAVVRIERPTRLDRARSLGYKPKQGIIVVRVRVRRGGLRKSRPTGSKKPATLGVNKITMGKSIQRIAEERAARKYPNMEVLNSYWVGEDGKYKWYEVILVDPHHPAIKADKNLNWICTGKHKGRVFRGLTSAGKKGRGLRNKGKGAEKVRPSIKAHGRRGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCL6</accession>
    <name>F6BCL6_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uroporphyrinogen decarboxylase (URO-D)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0677</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96227.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798830.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0677"/>
    <dbReference type="EnsemblBacteria" id="AEF96227">
        <property type="protein sequence ID" value="AEF96227"/>
        <property type="gene ID" value="Metig_0677"/>
    </dbReference>
    <dbReference type="GeneID" id="10643517"/>
    <dbReference type="KEGG" id="mig:Metig_0677"/>
    <dbReference type="HOGENOM" id="CLU_040933_2_0_2"/>
    <dbReference type="OMA" id="YERYCAP"/>
    <dbReference type="OrthoDB" id="21172at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004853">
        <property type="term" value="F:uroporphyrinogen decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006779">
        <property type="term" value="P:porphyrin-containing compound biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038071">
        <property type="entry name" value="UROD/MetE-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000257">
        <property type="entry name" value="Uroporphyrinogen_deCOase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01208">
        <property type="entry name" value="URO-D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51726">
        <property type="entry name" value="SSF51726"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Uroporphyrinogen_deCOase" evidence="1">
        <location>
            <begin position="3"/>
            <end position="330"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96227.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="336" mass="36680" checksum="070070586DFEA860" modified="2011-07-27" version="1">MIPKERLLGVITGSGIDRIPAVPLIGNFAGKISGYKVSEIARDGIKLAKAIEYTYKKMNPDLVTILTDLCIEAEAMGCELEYFDNDVPIVKKHVSLEEISIPDPEKDGRMPEFLKCIEYLVEKIGNEVFIGMCVPGPFTIAGNLHGTESMMMDLVMNPDVIKEEIKLATEASKLFINACMDRGAIPLICDPIASGSLISPKFYEEFALNPCKEIVKEIHKRGLPAILHICGDVNPILELMVKTGADFLSVDANVNLKEMKEKVGKNVGIIGNIDPGSTIYLGSKEDVLNECKKAIEYASDNPKGYILASGCEIPLASKEENVKAIVEAAELYGEVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCN7</accession>
    <name>F6BCN7_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0215 protein Metig_0698</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0698</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0215 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96248.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798851.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0698"/>
    <dbReference type="EnsemblBacteria" id="AEF96248">
        <property type="protein sequence ID" value="AEF96248"/>
        <property type="gene ID" value="Metig_0698"/>
    </dbReference>
    <dbReference type="GeneID" id="10643538"/>
    <dbReference type="KEGG" id="mig:Metig_0698"/>
    <dbReference type="HOGENOM" id="CLU_095956_1_0_2"/>
    <dbReference type="OMA" id="LRLAHMI"/>
    <dbReference type="OrthoDB" id="103162at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00582">
        <property type="entry name" value="UPF0215"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002802">
        <property type="entry name" value="DUF99"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39518">
        <property type="entry name" value="PTHR39518"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01949">
        <property type="entry name" value="DUF99"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006380">
        <property type="entry name" value="UCP006380"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96248.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="180" mass="20754" checksum="6890B7D380FB7363" modified="2011-07-27" version="1">MKDEIGVIGFDDAPFYKNDKTALLIATYFRGNRILDGVYFKKIQKDGKDATEKIIEVVKGKHYPKINAIFLYGVTFGGFNIADIFKINEETKKPVIVVIRKNPNRIDMINALRKYFDDWEERAKLLNSFPEPEPLEGIYVQYVGIDREGVKELIKKTRLKSKVPECLRVAHLIGRGFLEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCQ3</accession>
    <name>F6BCQ3_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Nitrogen regulatory protein P-II</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_0714</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the P(II) protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96264.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798867.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0714"/>
    <dbReference type="EnsemblBacteria" id="AEF96264">
        <property type="protein sequence ID" value="AEF96264"/>
        <property type="gene ID" value="Metig_0714"/>
    </dbReference>
    <dbReference type="GeneID" id="10643554"/>
    <dbReference type="KEGG" id="mig:Metig_0714"/>
    <dbReference type="HOGENOM" id="CLU_082268_0_0_2"/>
    <dbReference type="OMA" id="HQIEVNF"/>
    <dbReference type="OrthoDB" id="113533at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030234">
        <property type="term" value="F:enzyme regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006808">
        <property type="term" value="P:regulation of nitrogen utilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002187">
        <property type="entry name" value="N-reg_PII"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011322">
        <property type="entry name" value="N-reg_PII-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015867">
        <property type="entry name" value="N-reg_PII/ATP_PRibTrfase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017918">
        <property type="entry name" value="N-reg_PII_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30115">
        <property type="entry name" value="PTHR30115"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00543">
        <property type="entry name" value="P-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039144">
        <property type="entry name" value="GlnB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00340">
        <property type="entry name" value="PIIGLNB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00938">
        <property type="entry name" value="P-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54913">
        <property type="entry name" value="SSF54913"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00638">
        <property type="entry name" value="PII_GLNB_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51343">
        <property type="entry name" value="PII_GLNB_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="modified residue" description="O-UMP-tyrosine" evidence="1">
        <location>
            <position position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR602187-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF96264.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="112" mass="12599" checksum="54707A0730B914D2" modified="2011-07-27" version="1">MKKIEAIIRPSKLEDVKNALKDAGFIGMTVSEVKGRGVQGGIVERYRGREYTVDLLPKVKIEIVVKDEDVEKVIDIICENAKTGEFGDGKIFVIPVEEVIRVRTKERNEKAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCQ4</accession>
    <name>F6BCQ4_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">tRNA-splicing endonuclease</fullName>
            <ecNumber evidence="1">4.6.1.16</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">tRNA-intron endonuclease</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">endA</name>
        <name evidence="4" type="ordered locus">Metig_0715</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Endonuclease that removes tRNA introns. Cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. Recognizes a pseudosymmetric substrate in which 2 bulged loops of 3 bases are separated by a stem of 4 bp.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homotetramer; although the tetramer contains four active sites, only two participate in the cleavage. Therefore, it should be considered as a dimer of dimers.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the tRNA-intron endonuclease family. Archaeal short subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.6.1.16" evidence="1"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96265.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0715"/>
    <dbReference type="EnsemblBacteria" id="AEF96265">
        <property type="protein sequence ID" value="AEF96265"/>
        <property type="gene ID" value="Metig_0715"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0715"/>
    <dbReference type="HOGENOM" id="CLU_114393_0_0_2"/>
    <dbReference type="OMA" id="KGPGIDH"/>
    <dbReference type="OrthoDB" id="98477at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000213">
        <property type="term" value="F:tRNA-intron endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006388">
        <property type="term" value="P:tRNA splicing, via endonucleolytic cleavage and ligation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1350.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01833">
        <property type="entry name" value="EndA_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011856">
        <property type="entry name" value="tRNA_endonuc-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036167">
        <property type="entry name" value="tRNA_intron_Endo_cat-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006677">
        <property type="entry name" value="tRNA_intron_Endonuc_cat-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006678">
        <property type="entry name" value="tRNA_intron_Endonuc_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036740">
        <property type="entry name" value="tRNA_intron_Endonuc_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006676">
        <property type="entry name" value="tRNA_splic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016442">
        <property type="entry name" value="tRNA_splic_arch_short"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21227">
        <property type="entry name" value="PTHR21227"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01974">
        <property type="entry name" value="tRNA_int_endo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02778">
        <property type="entry name" value="tRNA_int_endo_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005285">
        <property type="entry name" value="tRNA_splic_archaea"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53032">
        <property type="entry name" value="SSF53032"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55267">
        <property type="entry name" value="SSF55267"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00324">
        <property type="entry name" value="endA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-0540">Nuclease</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="tRNA_int_endo_N" evidence="3">
        <location>
            <begin position="8"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="domain" description="tRNA_int_endo" evidence="2">
        <location>
            <begin position="82"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="111"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="121"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEF96265.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="175" mass="20150" checksum="61191D4D865CAAED" modified="2011-07-27" version="1">MAKKQIVGILSDDRVVVFDKNGISRLCSRYYGEKNDDFLSLSLIEALYLTSLGWLVVKDSNGRLLSFEELYEYARNIEESLCIKYLVYKDLRNRGYTVKTGLKYGTDFRLYERGADIDKEHSVYLVKVFSEEKPCNISELTGFVRVAHSVRKKLLIAIVDADGDVVYYDMGFVRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCS2</accession>
    <name>F6BCS2_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">NMD3 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0733</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96283.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798886.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0733"/>
    <dbReference type="EnsemblBacteria" id="AEF96283">
        <property type="protein sequence ID" value="AEF96283"/>
        <property type="gene ID" value="Metig_0733"/>
    </dbReference>
    <dbReference type="GeneID" id="10643573"/>
    <dbReference type="KEGG" id="mig:Metig_0733"/>
    <dbReference type="HOGENOM" id="CLU_065087_0_0_2"/>
    <dbReference type="OMA" id="CHMCGSY"/>
    <dbReference type="OrthoDB" id="105851at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043023">
        <property type="term" value="F:ribosomal large subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039768">
        <property type="entry name" value="Nmd3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007064">
        <property type="entry name" value="Nmd3_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12746">
        <property type="entry name" value="PTHR12746"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04981">
        <property type="entry name" value="NMD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NMD3" evidence="1">
        <location>
            <begin position="5"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96283.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="369" mass="42618" checksum="7C00219FCDBE57B5" modified="2011-07-27" version="1">MKGFCYRCGIEDELIEGLCPVCYAMENPLIDVPDIVEIEVCHMCGSYKRRVWQNPKGNDVYEILDEIAYFATKDNIEKHHPDIDVEIIPHEPRQLPGGKRSRVEIPVTVIATGKLAGEKQEREEKKDIVVHLKMVQCPRCSRYKSNYYVATLQVRAMNRFLTDEEVEELDKFIREEVAKRLEKDRMAFITKFTRLKEGIDYQMGSMGGARNIAQAIKARYGGKITETAKLVGVDKDTGKNQYRITIVVRIPEYKVGDVVEYNEKLHVVTSMNENKLYMLTMDNRRDKISLSWNEVEKHTKLVKKGDECSTSTVISVTKDNIIVMDDETYEVYELDKVMEIKEGDKVKIFKKDNVAYFIGKIEGKKLIFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCS8</accession>
    <name>F6BCS8_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_0741</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96289.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798892.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0741"/>
    <dbReference type="EnsemblBacteria" id="AEF96289">
        <property type="protein sequence ID" value="AEF96289"/>
        <property type="gene ID" value="Metig_0741"/>
    </dbReference>
    <dbReference type="GeneID" id="10643580"/>
    <dbReference type="KEGG" id="mig:Metig_0741"/>
    <dbReference type="HOGENOM" id="CLU_043239_0_0_2"/>
    <dbReference type="OrthoDB" id="8710at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002708">
        <property type="entry name" value="HcyBio"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016426">
        <property type="entry name" value="MA1821-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01837">
        <property type="entry name" value="HcyBio"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004698">
        <property type="entry name" value="UCP004698_CBS_MJ0100"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="SSF54631"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0129">CBS domain</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBS" evidence="2">
        <location>
            <begin position="394"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="2">
        <location>
            <begin position="455"/>
            <end position="511"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF96289.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="512" mass="56238" checksum="0B19335365B53024" modified="2011-07-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>F6BD14</accession>
    <name>F6BD14_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 8">Riboflavin synthase</fullName>
            <ecNumber evidence="4 8">2.5.1.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Metig_0830</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>2 6,7-dimethyl-8-(1-D-ribityl)lumazine + H(+) = 5-amino-6-(D-ribitylamino)uracil + riboflavin</text>
            <dbReference type="Rhea" id="RHEA:20772"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15934"/>
            <dbReference type="ChEBI" id="CHEBI:57986"/>
            <dbReference type="ChEBI" id="CHEBI:58201"/>
            <dbReference type="EC" id="2.5.1.9"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Cofactor biosynthesis; riboflavin biosynthesis; riboflavin from 2-hydroxy-3-oxobutyl phosphate and 5-amino-6-(D-ribitylamino)uracil: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the DMRL synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.9" evidence="4 8"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96375.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798977.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0830"/>
    <dbReference type="EnsemblBacteria" id="AEF96375">
        <property type="protein sequence ID" value="AEF96375"/>
        <property type="gene ID" value="Metig_0830"/>
    </dbReference>
    <dbReference type="GeneID" id="10643671"/>
    <dbReference type="KEGG" id="mig:Metig_0830"/>
    <dbReference type="HOGENOM" id="CLU_1682776_0_0_2"/>
    <dbReference type="OMA" id="QLMTNTH"/>
    <dbReference type="OrthoDB" id="104564at2157"/>
    <dbReference type="UniPathway" id="UPA00275">
        <property type="reaction ID" value="UER00405"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009349">
        <property type="term" value="C:riboflavin synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004746">
        <property type="term" value="F:riboflavin synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009231">
        <property type="term" value="P:riboflavin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd09210">
        <property type="entry name" value="Riboflavin_synthase_archaeal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.960">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002180">
        <property type="entry name" value="LS/RS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036467">
        <property type="entry name" value="LS/RS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006399">
        <property type="entry name" value="Ribfl_synth_arc"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00885">
        <property type="entry name" value="DMRL_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015750">
        <property type="entry name" value="Ribfl_synth_arc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52121">
        <property type="entry name" value="SSF52121"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01506">
        <property type="entry name" value="ribC_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 8" id="KW-0686">Riboflavin biosynthesis</keyword>
    <keyword evidence="7 8 9" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR015750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AEF96375.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="156" mass="17497" checksum="2818E1CEA6A4EF8A" modified="2011-07-27" version="1">MVIKVGIVDTTFARVDMASAAIKKLNELTSKIKIVRYTVPGIKDLPVACKKLIEEEGCEIVMALGMPGKAEKDKVCAHEASQGLMLAQLMTNKHIIEVFVHEDEAKDDKELEWLAKRRAEEHAENVYYLLFKPEYLRKNAGKGLRQGFEDAGPARL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>F6BD60</accession>
    <name>F6BD60_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Probable cyclic pyranopterin monophosphate synthase</fullName>
            <ecNumber evidence="3">4.6.1.17</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Molybdenum cofactor biosynthesis protein C</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">moaC</name>
        <name evidence="5" type="ordered locus">Metig_0878</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the conversion of (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate to cyclic pyranopterin monophosphate (cPMP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>(8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate = cyclic pyranopterin phosphate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:49580"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:59648"/>
            <dbReference type="ChEBI" id="CHEBI:131766"/>
            <dbReference type="EC" id="4.6.1.17"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 3">Cofactor biosynthesis; molybdopterin biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homohexamer; trimer of dimers.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the MoaC family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="4.6.1.17" evidence="3"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96421.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799023.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0878"/>
    <dbReference type="EnsemblBacteria" id="AEF96421">
        <property type="protein sequence ID" value="AEF96421"/>
        <property type="gene ID" value="Metig_0878"/>
    </dbReference>
    <dbReference type="GeneID" id="10643722"/>
    <dbReference type="KEGG" id="mig:Metig_0878"/>
    <dbReference type="HOGENOM" id="CLU_074693_1_2_2"/>
    <dbReference type="OMA" id="IWDMVKS"/>
    <dbReference type="OrthoDB" id="104538at2157"/>
    <dbReference type="UniPathway" id="UPA00344"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061799">
        <property type="term" value="F:cyclic pyranopterin monophosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006777">
        <property type="term" value="P:Mo-molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01419">
        <property type="entry name" value="MoaC_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.640">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01224_A">
        <property type="entry name" value="MoaC_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023045">
        <property type="entry name" value="Mo_CF_biosynth-C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023047">
        <property type="entry name" value="Mo_CF_biosynth-C_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036522">
        <property type="entry name" value="MoaC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002820">
        <property type="entry name" value="Mopterin_CF_biosynth-C_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01967">
        <property type="entry name" value="MoaC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55040">
        <property type="entry name" value="SSF55040"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00581">
        <property type="entry name" value="moaC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0456">Lyase</keyword>
    <keyword evidence="2 3" id="KW-0501">Molybdenum cofactor biosynthesis</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MoaC" evidence="4">
        <location>
            <begin position="12"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="3">
        <location>
            <begin position="108"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="active site" evidence="3">
        <location>
            <position position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEF96421.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="154" mass="17194" checksum="DEF38D227E441CEB" modified="2011-07-27" version="1">MLTHIDEKGVKMVDISKKEDVERICVAEGYIKLKPSTIELIKRNEIVKGDVLTTAQIAAMMGVKNTSNLIPACHPLPITSVRVDFEVFDDKIKATVEVKTTYKTGIEMEALTGVSIALLTIWDMVKSVEKDENGQYPNTEIYGIRVVKKVKNNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>F6BD81</accession>
    <name>F6BD81_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">50S ribosomal protein L18</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">rpl18</name>
        <name evidence="6" type="ordered locus">Metig_0899</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">This is one of the proteins that binds and probably mediates the attachment of the 5S RNA into the large ribosomal subunit, where it forms part of the central protuberance.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Part of the 50S ribosomal subunit. Contacts the 5S and 23S rRNAs.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the universal ribosomal protein uL18 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96442.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799044.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0899"/>
    <dbReference type="EnsemblBacteria" id="AEF96442">
        <property type="protein sequence ID" value="AEF96442"/>
        <property type="gene ID" value="Metig_0899"/>
    </dbReference>
    <dbReference type="GeneID" id="10643743"/>
    <dbReference type="KEGG" id="mig:Metig_0899"/>
    <dbReference type="HOGENOM" id="CLU_056222_2_0_2"/>
    <dbReference type="OMA" id="MAHGPRY"/>
    <dbReference type="OrthoDB" id="108717at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008097">
        <property type="term" value="F:5S rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01337_A">
        <property type="entry name" value="Ribosomal_L18_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005485">
        <property type="entry name" value="Rbsml_L5_euk/L18_arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23410">
        <property type="entry name" value="PTHR23410"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17144">
        <property type="entry name" value="Ribosomal_L5e"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 5 6" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2 5" id="KW-0699">rRNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEF96442.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="196" mass="22375" checksum="BE98723438809A85" modified="2011-07-27" version="1">MARDAKYRVPFRRRREGKTNYRKRLKLLLSGKPRLVARRTLNHIIAQVILYDEKGDRTVVSAHSRELIKLGYKGHCGNVPAAYLTGLLLGRKAISKGYKEAVLDIGLHRATKGAAVFAVLKGALDAGMEIPHGEEILPDESRIRGEHIKIYAELLKSEDEEKYKRQFSKYLEKGLKPEDLPEHFDEIKEKIMSLEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>F6BD84</accession>
    <name>F6BD84_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">50S ribosomal protein L6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">rpl6</name>
        <name evidence="5" type="ordered locus">Metig_0902</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">This protein binds to the 23S rRNA, and is important in its secondary structure. It is located near the subunit interface in the base of the L7/L12 stalk, and near the tRNA binding site of the peptidyltransferase center.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the universal ribosomal protein uL6 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96445.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799047.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0902"/>
    <dbReference type="EnsemblBacteria" id="AEF96445">
        <property type="protein sequence ID" value="AEF96445"/>
        <property type="gene ID" value="Metig_0902"/>
    </dbReference>
    <dbReference type="GeneID" id="10643746"/>
    <dbReference type="KEGG" id="mig:Metig_0902"/>
    <dbReference type="HOGENOM" id="CLU_065464_0_0_2"/>
    <dbReference type="OMA" id="VFQDGIY"/>
    <dbReference type="OrthoDB" id="80629at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.930.12">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01365_A">
        <property type="entry name" value="Ribosomal_L6_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000702">
        <property type="entry name" value="Ribosomal_L6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020040">
        <property type="entry name" value="Ribosomal_L6_a/b-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036789">
        <property type="entry name" value="Ribosomal_L6_a/b-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002359">
        <property type="entry name" value="Ribosomal_L6_CS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019907">
        <property type="entry name" value="Ribosomal_L6P_arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11655">
        <property type="entry name" value="PTHR11655"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00347">
        <property type="entry name" value="Ribosomal_L6"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002162">
        <property type="entry name" value="Ribosomal_L6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56053">
        <property type="entry name" value="SSF56053"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03653">
        <property type="entry name" value="uL6_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00700">
        <property type="entry name" value="RIBOSOMAL_L6_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="1 3 5" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="Ribosomal_L6" evidence="4">
        <location>
            <begin position="13"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="domain" description="Ribosomal_L6" evidence="4">
        <location>
            <begin position="97"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEF96445.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="182" mass="20356" checksum="CE547B95EFEF04CA" modified="2011-07-27" version="1">MPVAAFIREEIEIPENVTVDIVGNEVIVKSGNKELRRVLSYPGVEIKKEDNKVVIECEFPRKKQAAVIGTFKAHIKNMIKGVTEGFTYRLKIRYAHFPMKVTVKGNEVIIDNFLGEKHPRRAKILEGVTVKVSGEDVIVTGIDKEKVGQTAANIEQATKIKGRDPRVFQDGIYIVEKAGKVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDA5</accession>
    <name>F6BDA5_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA repair protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metig_0923</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in DNA damage repair.</text>
    </comment>
    <comment type="domain">
        <text evidence="1">Contains a predicted C4 metal binding domain at the N-terminus, which could be a zinc finger DNA binding domain.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the Nre family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96466.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0923"/>
    <dbReference type="EnsemblBacteria" id="AEF96466">
        <property type="protein sequence ID" value="AEF96466"/>
        <property type="gene ID" value="Metig_0923"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0923"/>
    <dbReference type="HOGENOM" id="CLU_039703_0_0_2"/>
    <dbReference type="OMA" id="PVGVWQI"/>
    <dbReference type="OrthoDB" id="17407at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02096">
        <property type="entry name" value="Nre"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033167">
        <property type="entry name" value="Nre"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006979">
        <property type="entry name" value="Nre_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006978">
        <property type="entry name" value="Nre_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38136">
        <property type="entry name" value="PTHR38136"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04895">
        <property type="entry name" value="Nre_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04894">
        <property type="entry name" value="Nre_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0227">DNA damage</keyword>
    <keyword evidence="1" id="KW-0234">DNA repair</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Nre_N" evidence="2">
        <location>
            <begin position="17"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="domain" description="Nre_C" evidence="3">
        <location>
            <begin position="286"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C4-type" evidence="1">
        <location>
            <begin position="8"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEF96466.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="374" mass="43172" checksum="675FF08674DEFE19" modified="2011-07-27" version="1">MLGDILRCIICKARGYCGRPFCPILQKINKKFKCSDLLENLPNVDMDFFGSNVSFFVGRWGYPNVKISPLITDIPKDVEHLFGYNLAEIIKIRINLLSPSVAANVNKRTTIIEKLQELAMSKEGVDTEVEFYKKPKLSLSFVDNLPPMGPYGELKRISYDNPKIPKIVDKVADDYSFEGVLRIYEKFDEIYASRLFSAGILGRKKKLVPTRWAITAVDDMIGKYLIKKIREYKILEEPLMFETSYLGNEFRIYFLPSAWAFELIEIYNKCVWNPTNKPITVSDYEIKERKTYAKNTGGAYYAARLAVLEKLDEMRRQAKVIVKRVVNEEYSVPLGVWVIREAVRDALRKRPKKLDNVEKIGTISSQKLLDDFIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-06-02" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDB8</accession>
    <name>F6BDB8_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">UPF0056 membrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Metig_0936</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cell membrane</location>
            <topology evidence="7">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the UPF0056 (MarC) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96479.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799081.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96479">
        <property type="protein sequence ID" value="AEF96479"/>
        <property type="gene ID" value="Metig_0936"/>
    </dbReference>
    <dbReference type="GeneID" id="10643782"/>
    <dbReference type="KEGG" id="mig:Metig_0936"/>
    <dbReference type="HOGENOM" id="CLU_079909_1_0_2"/>
    <dbReference type="OMA" id="LFVIMDP"/>
    <dbReference type="OrthoDB" id="88537at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002771">
        <property type="entry name" value="Multi_antbiot-R_MarC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33508">
        <property type="entry name" value="PTHR33508"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01914">
        <property type="entry name" value="MarC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00427">
        <property type="entry name" value="TIGR00427"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="47"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="73"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="113"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="146"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="184"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009784"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU362048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEF96479.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="206" mass="22577" checksum="C77DBB23C90770B5" modified="2011-07-27" version="1">MDYQFALLALTSLFAIINPVGLVPVFMAITAQYTHEERLRIIKKTTIATLIILISFALLGKYILLFFGISIQAFRIAGGILLFTISMDMLHGKISRVRHSKTEQEIASELEDIAIIPLAMPLLAGPGAITSVIVLMTKASSYDDKLAVILAILTCVLSTYAILKSAEKIEDKLSPIGIKIMTRMMGLMLAAISVQMILDGIKDVFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDD1</accession>
    <name>F6BDD1_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Bifunctional NAD(P)H-hydrate repair enzyme</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Nicotinamide nucleotide repair protein</fullName>
        </alternativeName>
        <domain>
            <recommendedName>
                <fullName evidence="11">ADP-dependent (S)-NAD(P)H-hydrate dehydratase</fullName>
                <ecNumber evidence="11">4.2.1.136</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="11">ADP-dependent NAD(P)HX dehydratase</fullName>
            </alternativeName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="11">NAD(P)H-hydrate epimerase</fullName>
                <ecNumber evidence="11">5.1.99.6</ecNumber>
            </recommendedName>
        </domain>
    </protein>
    <gene>
        <name evidence="10" type="primary">nnrE</name>
        <name evidence="9" type="synonym">nnrD</name>
        <name evidence="13" type="ordered locus">Metig_0949</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8 11">Bifunctional enzyme that catalyzes the epimerization of the S- and R-forms of NAD(P)HX and the dehydration of the S-form of NAD(P)HX at the expense of ADP, which is converted to AMP. This allows the repair of both epimers of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration.</text>
    </comment>
    <comment type="function">
        <text evidence="9">Catalyzes the dehydration of the S-form of NAD(P)HX at the expense of ADP, which is converted to AMP. Together with NAD(P)HX epimerase, which catalyzes the epimerization of the S- and R-forms, the enzyme allows the repair of both epimers of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration.</text>
    </comment>
    <comment type="function">
        <text evidence="10">Catalyzes the epimerization of the S- and R-forms of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration. This is a prerequisite for the S-specific NAD(P)H-hydrate dehydratase to allow the repair of both epimers of NAD(P)HX.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 10 11">
            <text>(6R)-NADHX = (6S)-NADHX</text>
            <dbReference type="Rhea" id="RHEA:32215"/>
            <dbReference type="ChEBI" id="CHEBI:64074"/>
            <dbReference type="ChEBI" id="CHEBI:64075"/>
            <dbReference type="EC" id="5.1.99.6"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 10 11">
            <text>(6R)-NADPHX = (6S)-NADPHX</text>
            <dbReference type="Rhea" id="RHEA:32227"/>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
            <dbReference type="ChEBI" id="CHEBI:64077"/>
            <dbReference type="EC" id="5.1.99.6"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3 9 11">
            <text>(6S)-NADHX + ADP = AMP + H(+) + NADH + phosphate</text>
            <dbReference type="Rhea" id="RHEA:32223"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:64074"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="4.2.1.136"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4 9 11">
            <text>(6S)-NADPHX + ADP = AMP + H(+) + NADPH + phosphate</text>
            <dbReference type="Rhea" id="RHEA:32235"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="4.2.1.136"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10 11">
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </cofactor>
        <text evidence="10 11">Binds 1 potassium ion per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="9">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the NnrD/CARKD family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the NnrE/AIBP family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6 11">In the C-terminal section; belongs to the NnrD/CARKD family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5 11">In the N-terminal section; belongs to the NnrE/AIBP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.136" evidence="11"/>
    <dbReference type="EC" id="5.1.99.6" evidence="11"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96492.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799094.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0949"/>
    <dbReference type="EnsemblBacteria" id="AEF96492">
        <property type="protein sequence ID" value="AEF96492"/>
        <property type="gene ID" value="Metig_0949"/>
    </dbReference>
    <dbReference type="GeneID" id="10643795"/>
    <dbReference type="KEGG" id="mig:Metig_0949"/>
    <dbReference type="HOGENOM" id="CLU_024853_4_1_2"/>
    <dbReference type="OMA" id="HNGGDAL"/>
    <dbReference type="OrthoDB" id="41305at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052855">
        <property type="term" value="F:ADP-dependent NAD(P)H-hydrate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052856">
        <property type="term" value="F:NADHX epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052857">
        <property type="term" value="F:NADPHX epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046496">
        <property type="term" value="P:nicotinamide nucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01171">
        <property type="entry name" value="YXKO-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01965">
        <property type="entry name" value="NADHX_dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01966">
        <property type="entry name" value="NADHX_epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000631">
        <property type="entry name" value="CARKD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030677">
        <property type="entry name" value="Nnr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004443">
        <property type="entry name" value="YjeF_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036652">
        <property type="entry name" value="YjeF_N_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01256">
        <property type="entry name" value="Carb_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03853">
        <property type="entry name" value="YjeF_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017184">
        <property type="entry name" value="Nnr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="SSF53613"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64153">
        <property type="entry name" value="SSF64153"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00196">
        <property type="entry name" value="yjeF_cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00197">
        <property type="entry name" value="yjeF_nterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51383">
        <property type="entry name" value="YJEF_C_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51385">
        <property type="entry name" value="YJEF_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 10 11" id="KW-0413">Isomerase</keyword>
    <keyword evidence="9 11" id="KW-0456">Lyase</keyword>
    <keyword evidence="10 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 11" id="KW-0520">NAD</keyword>
    <keyword evidence="9 11" id="KW-0521">NADP</keyword>
    <keyword evidence="9 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10 11" id="KW-0630">Potassium</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="YjeF N-terminal" evidence="12">
        <location>
            <begin position="25"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="region of interest" description="NADPHX" evidence="10">
        <location>
            <begin position="74"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="NADPHX" evidence="10">
        <location>
            <begin position="153"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="75"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="149"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="164"/>
        </location>
        <ligand>
            <name>(6S)-NADPHX</name>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="187"/>
        </location>
        <ligand>
            <name>(6S)-NADPHX</name>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="190"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="262"/>
        </location>
        <ligand>
            <name>(6S)-NADPHX</name>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="327"/>
        </location>
        <ligand>
            <name>(6S)-NADPHX</name>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="376"/>
        </location>
        <ligand>
            <name>(6S)-NADPHX</name>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="426"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="427"/>
        </location>
        <ligand>
            <name>(6S)-NADPHX</name>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00000909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00001241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00006001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00009524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00025153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR017184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AEF96492.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="485" mass="53765" checksum="2804E4B8D745CC0D" modified="2011-07-27" version="1">MDNLELFKILTKKIKEKDIISPKEMEIIDNNCEYLGISRILLMENAGKCVAEEVNKFKVELGEVPIFIFCGTGNNGGDGFAAARHLGNCSVILLGKEENIKTYEARENFKILKNLAEFGDISIKNVTHQNEILEILNEIENLDKAIIVDAMLGTGIRGELREPYKTVVEKLNEIKKKNDNIKIISVDVETGDLNADKVITFHKRKTINKENAIVKPIGIPKEAEYIVGWGDLKALSKRDPNSHKGENGKVLVIGGSKEFFGAPILTALAALKIADLVAVASVKDVVKKITNPELITYKLKGDYIGEKCIDKLIEISKKFDCIVLGNGLGVNEETKAFVNGFLERIGNKKVVIDADAIKVIEYEGFEFRENFIFTPHRREFEYMGINLDNLPSSTIVLKGRYDVIFNKNNVKINKTGNSGMTVGGTGDILCGIIGGLYAKNEAFLSACCGCFINGYAGDLLLKEKGYYYTPIDIIEKIPHVLKLFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDF6</accession>
    <name>F6BDF6_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0974</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96517.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799119.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0974"/>
    <dbReference type="EnsemblBacteria" id="AEF96517">
        <property type="protein sequence ID" value="AEF96517"/>
        <property type="gene ID" value="Metig_0974"/>
    </dbReference>
    <dbReference type="GeneID" id="10643820"/>
    <dbReference type="KEGG" id="mig:Metig_0974"/>
    <dbReference type="HOGENOM" id="CLU_732836_0_0_2"/>
    <dbReference type="OMA" id="FEVYNIY"/>
    <dbReference type="OrthoDB" id="34854at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007509">
        <property type="entry name" value="DUF515"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04415">
        <property type="entry name" value="DUF515"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="30"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96517.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="377" mass="42793" checksum="925198280E94F24D" modified="2011-07-27" version="1">MATGEFTGKIKKLKSKTKKSTKIPTEGLKLVLVVLIFVVIAFAGYNVYITSKSRELLKLENEKQTAIDTLNKLFANYPGDPEKIVFVKKIQHSKSTEEIDKVLDEARAYLQLKNYKDLTIQQIKDMYGNYFSQSLKAQELVRKISGAKSKEEVDRLFKSANIKEDIKNIINKQIDEVLASGDEYYYVVKDNKSEFMSREEIEKYRNIWSLSELKTLYIEPVSQLSKVAIKISAKQCGKIPHKKDIISIYSKDGSFICYGIVNSSYVILPAISYSEDKSASSSVNEYGDSYSSSSTSSISYSLNNLPGILHATVIDRLDYNAIKEKFGKYGEKLNKIEDETQIFDENVDYFLIISIPSDKIPDIIQIDPKDMVIVVKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDJ3</accession>
    <name>F6BDJ3_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1012</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96554.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799156.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1012"/>
    <dbReference type="EnsemblBacteria" id="AEF96554">
        <property type="protein sequence ID" value="AEF96554"/>
        <property type="gene ID" value="Metig_1012"/>
    </dbReference>
    <dbReference type="GeneID" id="10643866"/>
    <dbReference type="KEGG" id="mig:Metig_1012"/>
    <dbReference type="HOGENOM" id="CLU_149108_3_0_2"/>
    <dbReference type="OMA" id="PEITTNM"/>
    <dbReference type="OrthoDB" id="127809at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002849">
        <property type="entry name" value="DUF131"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01998">
        <property type="entry name" value="DUF131"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00304">
        <property type="entry name" value="TIGR00304"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="64"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96554.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="90" mass="10158" checksum="620F3059905ABDC4" modified="2011-07-27" version="1">MKPMLIFLGTILIFIGFFVVTLGMILPSEKTEKNIHEEDKKDIQYSGIVMIGPIPIVFGNSPGLMILSVLILLAMILWVFLFYMSVKIPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDJ4</accession>
    <name>F6BDJ4_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="5">MCM family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Metig_1013</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the MCM family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96555.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799157.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1013"/>
    <dbReference type="EnsemblBacteria" id="AEF96555">
        <property type="protein sequence ID" value="AEF96555"/>
        <property type="gene ID" value="Metig_1013"/>
    </dbReference>
    <dbReference type="GeneID" id="10643867"/>
    <dbReference type="KEGG" id="mig:Metig_1013"/>
    <dbReference type="HOGENOM" id="CLU_000995_7_2_2"/>
    <dbReference type="OMA" id="MHAKARL"/>
    <dbReference type="OrthoDB" id="3067at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032508">
        <property type="term" value="P:DNA duplex unwinding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031327">
        <property type="entry name" value="MCM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018525">
        <property type="entry name" value="MCM_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001208">
        <property type="entry name" value="MCM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041562">
        <property type="entry name" value="MCM_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033762">
        <property type="entry name" value="MCM_OB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11630">
        <property type="entry name" value="PTHR11630"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00493">
        <property type="entry name" value="MCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17855">
        <property type="entry name" value="MCM_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17207">
        <property type="entry name" value="MCM_OB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01657">
        <property type="entry name" value="MCMFAMILY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00350">
        <property type="entry name" value="MCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00847">
        <property type="entry name" value="MCM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50051">
        <property type="entry name" value="MCM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1 2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MCM" evidence="4">
        <location>
            <begin position="277"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="646"/>
            <end position="673"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU004070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEF96555.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="705" mass="80140" checksum="C3272EE3E21BCD94" modified="2011-07-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDJ5</accession>
    <name>F6BDJ5_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">UPF0179 protein Metig_1014</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_1014</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the UPF0179 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96556.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799158.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1014"/>
    <dbReference type="EnsemblBacteria" id="AEF96556">
        <property type="protein sequence ID" value="AEF96556"/>
        <property type="gene ID" value="Metig_1014"/>
    </dbReference>
    <dbReference type="GeneID" id="10643868"/>
    <dbReference type="KEGG" id="mig:Metig_1014"/>
    <dbReference type="HOGENOM" id="CLU_121764_0_0_2"/>
    <dbReference type="OMA" id="CPSHEYC"/>
    <dbReference type="OrthoDB" id="108341at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00498">
        <property type="entry name" value="UPF0179"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005369">
        <property type="entry name" value="UPF0179"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40699">
        <property type="entry name" value="PTHR40699"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03684">
        <property type="entry name" value="UPF0179"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006595">
        <property type="entry name" value="UCP006595"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF96556.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="148" mass="16753" checksum="19A772E08E0C8C4B" modified="2011-07-27" version="1">MKKITLIGSKLAKMGNEFVYMGILEECETCKFKRICHNNLEVGGRYKIVSVRSANHQCKIHEDGVKVVEVVPAEFVIMVEAKKALEGVTLTHTPINCENIFCENYTYCNPEGISEGDKYKILSVINEKIKCEKGLSLRKVVITLERQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDL3</accession>
    <name>F6BDL3_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Deoxyribonuclease/rho motif-related TRAM</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1032</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96574.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1032"/>
    <dbReference type="EnsemblBacteria" id="AEF96574">
        <property type="protein sequence ID" value="AEF96574"/>
        <property type="gene ID" value="Metig_1032"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_1032"/>
    <dbReference type="HOGENOM" id="CLU_182721_0_0_2"/>
    <dbReference type="OMA" id="FMFREES"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002792">
        <property type="entry name" value="TRAM_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01938">
        <property type="entry name" value="TRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50926">
        <property type="entry name" value="TRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TRAM" evidence="1">
        <location>
            <begin position="29"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96574.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="86" mass="9885" checksum="F3DDC330B5A492CE" modified="2011-07-27" version="1">MDIYKLYTWNMVNEMVFNRNKRNMDRNVPVKEGQEYTVMIEDMGRGGDGIARIEGFVVFVPDTKKGDTVSIKITSVKSKFAFAEKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDN7</accession>
    <name>F6BDN7_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Nucleic acid binding OB-fold tRNA/helicase-type</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1059</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96598.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799199.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1059"/>
    <dbReference type="EnsemblBacteria" id="AEF96598">
        <property type="protein sequence ID" value="AEF96598"/>
        <property type="gene ID" value="Metig_1059"/>
    </dbReference>
    <dbReference type="GeneID" id="10643913"/>
    <dbReference type="KEGG" id="mig:Metig_1059"/>
    <dbReference type="HOGENOM" id="CLU_1954710_0_0_2"/>
    <dbReference type="OMA" id="GQWESNY"/>
    <dbReference type="OrthoDB" id="94614at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004365">
        <property type="entry name" value="NA-bd_OB_tRNA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01336">
        <property type="entry name" value="tRNA_anti-codon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0347">Helicase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="OB" evidence="1">
        <location>
            <begin position="41"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96598.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="137" mass="15509" checksum="F2F2E88CCDA2B136" modified="2011-07-27" version="1">MRLTAKRIPLKALDEGNYVVTEGKWESNYLEHPVYGKVSRVAIYGVVISKYENKAKEVCSITVDDFTGDVRVVGFRGMAKYLEKFDIGDIVLVVGKLKKGIRDELYISPEIVRRVSINHLFLNAIENFKVEGDIGEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDP4</accession>
    <name>F6BDP4_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="4">YcfA family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metig_1066</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96605.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96605">
        <property type="protein sequence ID" value="AEF96605"/>
        <property type="gene ID" value="Metig_1066"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_1066"/>
    <dbReference type="HOGENOM" id="CLU_164851_6_2_2"/>
    <dbReference type="OMA" id="MSPKYPV"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.920.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012933">
        <property type="entry name" value="HicA_mRNA_interferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038570">
        <property type="entry name" value="HicA_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07927">
        <property type="entry name" value="HicA_toxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0540">Nuclease</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-1277">Toxin-antitoxin system</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEF96605.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="81" mass="9411" checksum="BF6ED055E07321EF" modified="2011-07-27" version="1">MLPKLPVVGGKDLIKFLKKLGYEVVRQRGSHVRLRKETELGIHNITIPYHEEIARGTLNNILTDISKWNNIPKEELIKRLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDP9</accession>
    <name>F6BDP9_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1071</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96610.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799211.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1071"/>
    <dbReference type="EnsemblBacteria" id="AEF96610">
        <property type="protein sequence ID" value="AEF96610"/>
        <property type="gene ID" value="Metig_1071"/>
    </dbReference>
    <dbReference type="GeneID" id="10643925"/>
    <dbReference type="KEGG" id="mig:Metig_1071"/>
    <dbReference type="HOGENOM" id="CLU_540387_0_0_2"/>
    <dbReference type="OMA" id="QYLEPSK"/>
    <dbReference type="OrthoDB" id="17668at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="468"/>
            <end position="488"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96610.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="497" mass="56580" checksum="936E94500E2FE89A" modified="2011-07-27" version="1">MKKSSFIFLTFLSLLLILNTINAIEFTSFDYKNEYLEPGKTYDLWVVITPEKEINNTIIGIYPYGISKEYIQIIKGKDYVGHLFQSEQGVGHFIIKIKDNAPSKDYKIVAYCNYTENGEQYSENRIFEIPVRGEAIIEIQNPPILKEGTNKIFLRITNKGTGIAENIKITFENGKNIYALSNSYVINYLKPGETKLIRLILYANGEIGELPYTITYQNQYNLLELTDKTETDTSTTSTYKNQKTIEEKGTLTFKIVPNDLISINLKNITCPVGKINNLTILIRNNYKDANFIITIGKYYLGNNQKTIFIKKGETKNVSFKIKINEMGIKEIPIKIYFDENEINKNLTINVIGKAELVLTGVNVEGFGEKIITGDLSNIGTAPAKSVLISIKKTKNIIPKRPYENYFIGTLNPDDYGSFELHCQINGTVNEIPIVITYRDENNNLVTIYKTIKINGDVISLKNNNKEEVNYLVIGIAIVFCFGVVYLIYRGFVRKDEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-09-29" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDQ6</accession>
    <name>F6BDQ6_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="3">AAA ATPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_1078</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the archaeal ATPase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96617.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96617">
        <property type="protein sequence ID" value="AEF96617"/>
        <property type="gene ID" value="Metig_1078"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_1078"/>
    <dbReference type="HOGENOM" id="CLU_068608_0_0_2"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011579">
        <property type="entry name" value="ATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01637">
        <property type="entry name" value="ATPase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA" evidence="2">
        <location>
            <begin position="22"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF96617.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="337" mass="40257" checksum="C353AD0136071D6D" modified="2011-07-27" version="1">MIKFYDREKELNYLKTYCQLIPNSILFIYGPKSSGKSTVMKRVIKDLEDREDLVFFYYNLRKYATTTKEEFLNVFFEKGDKKYLRNTLELNFKIFKFGVDEDYDFKNVSLNDVFRKINEDINAVVEEGKKPILIIDELQKLKSIYFNGNKSLLNELFNLFVSLTKMEHLCHVICLTSDSLFINEVYSNSSLAETSKFYLVDWLNKEDIKKILKEENFNKREINYAISYISLPYEIFDLVENKKLGVSVEETIRNWINVEEERIRYFIRTNLNKKEEILETLSLFEDDLYIKSKYIDDELFSTFKMLIESEILFYDVVNGIIKPTSLKKWYAIKNIIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDQ9</accession>
    <name>F6BDQ9_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">DNA polymerase II small subunit</fullName>
            <shortName evidence="12">Pol II</shortName>
            <ecNumber evidence="12">2.7.7.7</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Exodeoxyribonuclease small subunit</fullName>
            <ecNumber evidence="12">3.1.11.1</ecNumber>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">polB</name>
        <name evidence="15" type="ordered locus">Metig_1081</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11 12">Possesses two activities: a DNA synthesis (polymerase) and an exonucleolytic activity that degrades single-stranded DNA in the 3' to 5' direction. Has a template-primer preference which is characteristic of a replicative DNA polymerase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 12">
            <text>Exonucleolytic cleavage in the 3'- to 5'-direction to yield nucleoside 5'-phosphates.</text>
            <dbReference type="EC" id="3.1.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 12">
            <text>a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) = diphosphate + DNA(n+1)</text>
            <dbReference type="Rhea" id="RHEA:22508"/>
            <dbReference type="Rhea" id="RHEA-COMP:17339"/>
            <dbReference type="Rhea" id="RHEA-COMP:17340"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:173112"/>
            <dbReference type="EC" id="2.7.7.7"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="3 12">Heterodimer of a large subunit and a small subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the DNA polymerase delta/II small subunit family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.7" evidence="12"/>
    <dbReference type="EC" id="3.1.11.1" evidence="12"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96620.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799221.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1081"/>
    <dbReference type="EnsemblBacteria" id="AEF96620">
        <property type="protein sequence ID" value="AEF96620"/>
        <property type="gene ID" value="Metig_1081"/>
    </dbReference>
    <dbReference type="GeneID" id="10643935"/>
    <dbReference type="KEGG" id="mig:Metig_1081"/>
    <dbReference type="HOGENOM" id="CLU_027850_1_0_2"/>
    <dbReference type="OMA" id="HVQAALI"/>
    <dbReference type="OrthoDB" id="24499at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008408">
        <property type="term" value="F:3'-5' exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008852">
        <property type="term" value="F:exodeoxyribonuclease I activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000738">
        <property type="term" value="P:DNA catabolic process, exonucleolytic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006261">
        <property type="term" value="P:DNA-templated DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00325">
        <property type="entry name" value="DNApol_II_A_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP_ApaH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024826">
        <property type="entry name" value="DNA_pol_delta/II_ssu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011149">
        <property type="entry name" value="Pol2_small_arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10416">
        <property type="entry name" value="PTHR10416"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000803">
        <property type="entry name" value="Arc_Pol2_small"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="SSF56300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6 12" id="KW-0235">DNA replication</keyword>
    <keyword evidence="8 12" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7 12 15" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="12" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9 12" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="12" id="KW-0540">Nuclease</keyword>
    <keyword evidence="5 12 15" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12 15" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Metallophos" evidence="14">
        <location>
            <begin position="308"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="86"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00024632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00024817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="AEF96620.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="577" mass="66624" checksum="1F03EEC0B9F12361" modified="2011-07-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDT8</accession>
    <name>F6BDT8_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Glycerol-1-phosphate dehydrogenase [NAD(P)+]</fullName>
            <shortName evidence="11">G1P dehydrogenase</shortName>
            <shortName evidence="11">G1PDH</shortName>
            <ecNumber evidence="11">1.1.1.261</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Enantiomeric glycerophosphate synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">sn-glycerol-1-phosphate dehydrogenase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">egsA</name>
        <name evidence="15" type="ordered locus">Metig_1110</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes the NAD(P)H-dependent reduction of dihydroxyacetonephosphate (DHAP or glycerone phosphate) to glycerol 1-phosphate (G1P). The G1P thus generated is used as the glycerophosphate backbone of phospholipids in the cellular membranes of Archaea.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>NAD(+) + sn-glycerol 1-phosphate = dihydroxyacetone phosphate + H(+) + NADH</text>
            <dbReference type="Rhea" id="RHEA:21412"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
            <dbReference type="ChEBI" id="CHEBI:57685"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.261"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>NADP(+) + sn-glycerol 1-phosphate = dihydroxyacetone phosphate + H(+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:21416"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
            <dbReference type="ChEBI" id="CHEBI:57685"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.261"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11 12">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="11 12">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="11">Membrane lipid metabolism; glycerophospholipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the glycerol-1-phosphate dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.261" evidence="11"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96649.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799250.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1110"/>
    <dbReference type="EnsemblBacteria" id="AEF96649">
        <property type="protein sequence ID" value="AEF96649"/>
        <property type="gene ID" value="Metig_1110"/>
    </dbReference>
    <dbReference type="GeneID" id="10643968"/>
    <dbReference type="KEGG" id="mig:Metig_1110"/>
    <dbReference type="HOGENOM" id="CLU_038362_0_0_2"/>
    <dbReference type="OMA" id="ALHGEQC"/>
    <dbReference type="OrthoDB" id="57545at2157"/>
    <dbReference type="UniPathway" id="UPA00940"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106357">
        <property type="term" value="F:glycerol-1-phosphate dehydrogenase [NAD+] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106358">
        <property type="term" value="F:glycerol-1-phosphate dehydrogenase [NADP+] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006650">
        <property type="term" value="P:glycerophospholipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00497_A">
        <property type="entry name" value="G1P_dehydrogenase_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023002">
        <property type="entry name" value="G1P_dehydrogenase_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032837">
        <property type="entry name" value="G1PDH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016205">
        <property type="entry name" value="Glycerol_DH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43616">
        <property type="entry name" value="PTHR43616"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13685">
        <property type="entry name" value="Fe-ADH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000112">
        <property type="entry name" value="Glycerol_dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 11" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="8 11" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="3 11 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 11 14" id="KW-0520">NAD</keyword>
    <keyword evidence="5 11" id="KW-0521">NADP</keyword>
    <keyword evidence="6 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9 11" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="10 11" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11 12" id="KW-0862">Zinc</keyword>
    <feature type="binding site" evidence="11 14">
        <location>
            <begin position="76"/>
            <end position="80"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11 14">
        <location>
            <begin position="98"/>
            <end position="101"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11 13">
        <location>
            <position position="103"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11 14">
        <location>
            <position position="107"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11 12 13">
        <location>
            <position position="146"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11 12 13">
        <location>
            <position position="224"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="228"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11 12 13">
        <location>
            <position position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR000112-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR000112-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR000112-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="AEF96649.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="333" mass="37281" checksum="6882D65173E44172" modified="2011-07-27" version="1">MIVIPRYILIKENAIYQTTDILKKLNLKNPLVITGKNTKKYCKFDFDIQYYDEIDIDEFEVSKYYKYDSIIGLGGGRAIDTGKLIAYKLGIPFLSIPTTASNDGIASPIVSIRQPSFMTEAPIAIIADINIIKKSPKRLLSAGMGDIVSNITAVLDWKLAHREIGEEYSESSAIFSKTIAKELIDYVLNSNLEEYPKKLVKSLIASGITISIAHSSRPASGSEHLFSHALDHLKKEYDLKINSLHGEQCGIGTIMMSYLHELENEKLKGLSEKIKLSLKKVNAPTTAKELEIDEDLIIEALTIAHKIRNRYTILRHGISKEKAKEIAEKTEVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDT9</accession>
    <name>F6BDT9_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Radical SAM domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Metig_1111</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>2Fe-2S cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49601"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96650.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1111"/>
    <dbReference type="EnsemblBacteria" id="AEF96650">
        <property type="protein sequence ID" value="AEF96650"/>
        <property type="gene ID" value="Metig_1111"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_1111"/>
    <dbReference type="HOGENOM" id="CLU_833177_0_0_2"/>
    <dbReference type="OMA" id="CKFCYMS"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004076">
        <property type="term" value="F:biotin synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009102">
        <property type="term" value="P:biotin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010722">
        <property type="entry name" value="BATS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002684">
        <property type="entry name" value="Biotin_synth/BioAB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaB/NifB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22976">
        <property type="entry name" value="PTHR22976"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00876">
        <property type="entry name" value="BATS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="7" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="8">
        <location>
            <begin position="24"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AEF96650.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="365" mass="41912" checksum="17024EBA76E53928" modified="2011-07-27" version="1">MVMKINSEEILENAIKAFKLTTKHHGNYTSLERALFLGWYCNLKNPCKFCYMSTQKNKIKDPLKARRRLETILAEAILMKRIGWKLEFISGGYGYTSKEINDIVEMVAYVQKSRQYLNVGLVDFDNLNLDVIEGVVGAVETVNEELHDYLCPDKPIEKIKDMLLKAKDLGLKTGITIILGLGEKEEDIEKLLNLIEELELDRITFYSLNPQKGTIFENKTSITTLEYMNWVSSVRLNFPKIKIITGTWVDKLTNIGPLIMSGSNTITKFPLFSMFGKKEGKTVEKEILSTGRELLGTFSDVEALMGKKKLKNSPYKDEDIEISEENLEKINNLQDEINKKVEFYVRKTLNKIAKETPDIEYSHMH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDW2</accession>
    <name>F6BDW2_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Transcriptional regulator, XRE family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Metig_1134</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96673.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799274.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1134"/>
    <dbReference type="EnsemblBacteria" id="AEF96673">
        <property type="protein sequence ID" value="AEF96673"/>
        <property type="gene ID" value="Metig_1134"/>
    </dbReference>
    <dbReference type="GeneID" id="10643992"/>
    <dbReference type="KEGG" id="mig:Metig_1134"/>
    <dbReference type="HOGENOM" id="CLU_005379_0_1_2"/>
    <dbReference type="OMA" id="NCMGTIH"/>
    <dbReference type="OrthoDB" id="9947at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016539">
        <property type="term" value="P:intein-mediated protein splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003586">
        <property type="entry name" value="Hint_dom_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003587">
        <property type="entry name" value="Hint_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036844">
        <property type="entry name" value="Hint_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006142">
        <property type="entry name" value="INTEIN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030934">
        <property type="entry name" value="Intein_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006141">
        <property type="entry name" value="Intein_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001482">
        <property type="entry name" value="T2SS/T4SS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00437">
        <property type="entry name" value="T2SSE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00379">
        <property type="entry name" value="INTEIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00305">
        <property type="entry name" value="HintC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00306">
        <property type="entry name" value="HintN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51294">
        <property type="entry name" value="SSF51294"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01443">
        <property type="entry name" value="intein_Cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01445">
        <property type="entry name" value="intein_Nterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50818">
        <property type="entry name" value="INTEIN_C_TER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50817">
        <property type="entry name" value="INTEIN_N_TER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0068">Autocatalytic cleavage</keyword>
    <keyword evidence="2" id="KW-0651">Protein splicing</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH cro/C1-type" evidence="5">
        <location>
            <begin position="523"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="domain" description="INTEIN_C_TER" evidence="4">
        <location>
            <begin position="627"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="10"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50818"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEF96673.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="799" mass="91547" checksum="E95B7EE6A54662BC" modified="2011-07-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDZ4</accession>
    <name>F6BDZ4_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_1167</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96705.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799305.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1167"/>
    <dbReference type="EnsemblBacteria" id="AEF96705">
        <property type="protein sequence ID" value="AEF96705"/>
        <property type="gene ID" value="Metig_1167"/>
    </dbReference>
    <dbReference type="GeneID" id="10644027"/>
    <dbReference type="KEGG" id="mig:Metig_1167"/>
    <dbReference type="HOGENOM" id="CLU_208223_0_0_2"/>
    <dbReference type="OMA" id="KVIMLQK"/>
    <dbReference type="OrthoDB" id="132044at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="32"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF96705.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="62" mass="7029" checksum="D7E060E2C11C23ED" modified="2011-07-27" version="1">MIPPEILVTLVVISIALNVVLFVKVVMLQRELNAVKKSTQLTKEEVEKLNERLKRLKMGGQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-06-02" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDZ9</accession>
    <name>F6BDZ9_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="3">D-glucuronyl C5-epimerase domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_1172</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96710.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799310.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96710">
        <property type="protein sequence ID" value="AEF96710"/>
        <property type="gene ID" value="Metig_1172"/>
    </dbReference>
    <dbReference type="GeneID" id="10644032"/>
    <dbReference type="KEGG" id="mig:Metig_1172"/>
    <dbReference type="HOGENOM" id="CLU_910928_0_0_2"/>
    <dbReference type="OMA" id="CQAGCLK"/>
    <dbReference type="OrthoDB" id="46709at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047464">
        <property type="term" value="F:heparosan-N-sulfate-glucuronate 5-epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015012">
        <property type="term" value="P:heparan sulfate proteoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010598">
        <property type="entry name" value="C5-epim_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039721">
        <property type="entry name" value="C5-epimerase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13174">
        <property type="entry name" value="PTHR13174"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06662">
        <property type="entry name" value="C5-epim_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="domain" description="C5-epim_C" evidence="2">
        <location>
            <begin position="132"/>
            <end position="309"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF06662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF96710.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="310" mass="36586" checksum="71818095F6A3C4BD" modified="2011-07-27" version="1">MLKNIKRYIVIPIAILSLALSFIFGYFTGISQITYSENPIIQYIENPKPFTVENVNMPVTYYGTINGKYIGYQITPHNVNDEARKCFYKYLKLKNEDQEKAKNCLKRGLFLTEYLISQSEEETVNLNGTNITFIIWRYNFDYPEYNLSKGWAGSLCQAGCLKTLYLAYNTTGDERYLILANKALNAFKVPVERGGLLKIRKRNNKTYYWYPEYASNNPPYVLNGFITSVIWIGEFANKTNNKNANFLYREGVKSIKAFISDYDDNDWSYYDALGHRCNKHYEHLHRLQMLWLYNLTKDEIFLKYYNKWKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>F6BE13</accession>
    <name>F6BE13_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Precorrin-2 C20-methyltransferase</fullName>
            <ecNumber evidence="10">2.1.1.151</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Metig_1186</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; adenosylcobalamin biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7 8">Belongs to the precorrin methyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.151" evidence="10"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96724.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799323.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1186"/>
    <dbReference type="EnsemblBacteria" id="AEF96724">
        <property type="protein sequence ID" value="AEF96724"/>
        <property type="gene ID" value="Metig_1186"/>
    </dbReference>
    <dbReference type="GeneID" id="10644046"/>
    <dbReference type="KEGG" id="mig:Metig_1186"/>
    <dbReference type="HOGENOM" id="CLU_076014_2_0_2"/>
    <dbReference type="OMA" id="MEYPVTT"/>
    <dbReference type="OrthoDB" id="83180at2157"/>
    <dbReference type="UniPathway" id="UPA00148"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043781">
        <property type="term" value="F:cobalt-factor II C20-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030788">
        <property type="term" value="F:precorrin-2 C20-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd11645">
        <property type="entry name" value="Precorrin_2_C20_MT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.950.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1010.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000878">
        <property type="entry name" value="4pyrrol_Mease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035996">
        <property type="entry name" value="4pyrrol_Methylase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014777">
        <property type="entry name" value="4pyrrole_Mease_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014776">
        <property type="entry name" value="4pyrrole_Mease_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012382">
        <property type="entry name" value="CobI/CbiL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006364">
        <property type="entry name" value="CobI/CbiL/CobIJ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003043">
        <property type="entry name" value="Uropor_MeTrfase_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00590">
        <property type="entry name" value="TP_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036427">
        <property type="entry name" value="Precrrn-2_mtase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53790">
        <property type="entry name" value="SSF53790"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01467">
        <property type="entry name" value="cobI_cbiL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00840">
        <property type="entry name" value="SUMT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword evidence="4 8 10" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5 8 10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="TP_methylase" evidence="9">
        <location>
            <begin position="4"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR036427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AEF96724.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="227" mass="25732" checksum="9AC7566C997435DC" modified="2011-07-27" version="1">MVKKVYGIGVGVGDKELLTLKAMNVLEKVNTVFVPISKEGKKSIALEIVKDYINGKKIIELLFPMTKDKEILKKYWNEATEKIMNEDGEVAVLTLGDPTLYSTFSYVWNLLKDNGVEVEIINGISSPFAAAGRLNIPLVEGDEKLCILPQGKDLERYLDEFDTIVVMKTTNLEEKLKELNDKKDKYLIGIVKKATFEDEKIAFGKIDEIDFDEFRDYLSIAIIKKVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>F6BE29</accession>
    <name>F6BE29_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Cell wall binding repeat 2-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_0003</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95565.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798175.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0003"/>
    <dbReference type="EnsemblBacteria" id="AEF95565">
        <property type="protein sequence ID" value="AEF95565"/>
        <property type="gene ID" value="Metig_0003"/>
    </dbReference>
    <dbReference type="GeneID" id="10642838"/>
    <dbReference type="KEGG" id="mig:Metig_0003"/>
    <dbReference type="HOGENOM" id="CLU_054697_2_0_2"/>
    <dbReference type="OrthoDB" id="48337at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="364"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="197"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="370"/>
            <end position="398"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF95565.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="398" mass="45713" checksum="5D40A6B68511508E" modified="2011-07-27" version="1">MVKRLLLLLLALLVPTVSATNVILVSDNQADYLTATNIASLFNDTEVVVAPWGIYNESVVNEILKLNPEQVIIIGGPVAVPDEYVEKLENLGIKVERIAGKDRYETNKEVIEKFKEKLKEKKVVVVHGDVGFLVNISKDTIVILTNGVNLTVDVDELEPKEVEVVEDPVANLTGIAKKLENHGLKVKINVVPEHALEKVLENRIKELKTKIKVLKKQGVDTEDLEDELKDLEEMLKEKNYDDAYKLMLEIKNKEMVKIKLKLHVHGKGNEKLKLELEVKHKKKGKEDEEEIENETEVEHEYENGSKYEYKYEYEYGNGTKYKYEHKYEYEENKYESEEEHEVETSINNTLVNESATIDTTLEIDDHELEESNEVDTHNDIGSNEVEIEHESETENEIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F6BE48</accession>
    <name>F6BE48_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Alpha-L-glutamate ligase, RimK family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_0022</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95584.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798194.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0022"/>
    <dbReference type="EnsemblBacteria" id="AEF95584">
        <property type="protein sequence ID" value="AEF95584"/>
        <property type="gene ID" value="Metig_0022"/>
    </dbReference>
    <dbReference type="GeneID" id="10642857"/>
    <dbReference type="KEGG" id="mig:Metig_0022"/>
    <dbReference type="HOGENOM" id="CLU_975255_0_0_2"/>
    <dbReference type="OMA" id="YEHKVFY"/>
    <dbReference type="OrthoDB" id="42812at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043774">
        <property type="term" value="F:coenzyme F420-2 alpha-glutamyl ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013651">
        <property type="entry name" value="ATP-grasp_RimK-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031039">
        <property type="entry name" value="F420_CofF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004666">
        <property type="entry name" value="RpS6_RimK/Lys_biosynth_LsyX"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08443">
        <property type="entry name" value="RimK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR04443">
        <property type="entry name" value="F420_CofF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00768">
        <property type="entry name" value="rimK_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-grasp" evidence="2">
        <location>
            <begin position="100"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF95584.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="288" mass="32660" checksum="1CE47C39D33B8310" modified="2011-07-27" version="1">MVKITIISAEGGSTIRALKNAIEKLGEKCEVFLLSDDNFLLDSGFNLESDLIHTRCGIGDYLDKLTLYSWQFLNALEAEGHLFINPLKTVYASSDKFKTIKILEKNKIPIPKTALIRDYDDAVKFMEKNNIDFPVVLKNSFSKCGLKTYLAKNHEELKKLTKNAIWESKLIQEFIDFKESGVYKDIRVLVVDGEVVGGYRRVSKDFRTNLHLSQGCVEKLQLNEEIEEIALKCAEVMNGYIMGVDILPKNGEYYVIEVNSAPGTKGFRQIGINADEKIAKCLIKYARR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>F6BE50</accession>
    <name>F6BE50_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Threonine--tRNA ligase</fullName>
            <ecNumber evidence="11">6.1.1.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Threonyl-tRNA synthetase</fullName>
            <shortName evidence="11">ThrRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">thrS</name>
        <name evidence="13" type="ordered locus">Metig_0024</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 11">
            <text>ATP + L-threonine + tRNA(Thr) = AMP + diphosphate + H(+) + L-threonyl-tRNA(Thr)</text>
            <dbReference type="Rhea" id="RHEA:24624"/>
            <dbReference type="Rhea" id="RHEA-COMP:9670"/>
            <dbReference type="Rhea" id="RHEA-COMP:9704"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78534"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="11">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 11">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.3" evidence="11"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95586.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798196.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0024"/>
    <dbReference type="EnsemblBacteria" id="AEF95586">
        <property type="protein sequence ID" value="AEF95586"/>
        <property type="gene ID" value="Metig_0024"/>
    </dbReference>
    <dbReference type="GeneID" id="10642859"/>
    <dbReference type="KEGG" id="mig:Metig_0024"/>
    <dbReference type="HOGENOM" id="CLU_029833_0_0_2"/>
    <dbReference type="OMA" id="IATFQID"/>
    <dbReference type="OrthoDB" id="11656at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002161">
        <property type="term" value="F:aminoacyl-tRNA editing activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004829">
        <property type="term" value="F:threonine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006435">
        <property type="term" value="P:threonyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.800">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.80.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00184">
        <property type="entry name" value="Thr_tRNA_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002314">
        <property type="entry name" value="aa-tRNA-synt_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004154">
        <property type="entry name" value="Anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036621">
        <property type="entry name" value="Anticodon-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023509">
        <property type="entry name" value="DTD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002320">
        <property type="entry name" value="Thr-tRNA-ligase_IIa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015011">
        <property type="entry name" value="Threonyl-tRNA_syn_edit_dom_arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11451">
        <property type="entry name" value="PTHR11451"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03129">
        <property type="entry name" value="HGTP_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00587">
        <property type="entry name" value="tRNA-synt_2b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08915">
        <property type="entry name" value="tRNA-Thr_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01047">
        <property type="entry name" value="TRNASYNTHTHR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="SSF55681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00418">
        <property type="entry name" value="thrS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 11 13" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="8 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 11 13" id="KW-0436">Ligase</keyword>
    <keyword evidence="5 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 11" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3 11" id="KW-0820">tRNA-binding</keyword>
    <keyword evidence="7 11" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="AA_TRNA_LIGASE_II" evidence="12">
        <location>
            <begin position="223"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="291"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="343"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="467"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AEF95586.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="622" mass="72443" checksum="7B43DA28A91DD64C" modified="2011-07-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>F6BE56</accession>
    <name>F6BE56_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Malate dehydrogenase</fullName>
            <ecNumber evidence="8">1.1.1.82</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Metig_0031</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the LDH/MDH superfamily.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.82" evidence="8"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95592.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798202.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="SMR" id="F6BE56"/>
    <dbReference type="STRING" id="880724.Metig_0031"/>
    <dbReference type="EnsemblBacteria" id="AEF95592">
        <property type="protein sequence ID" value="AEF95592"/>
        <property type="gene ID" value="Metig_0031"/>
    </dbReference>
    <dbReference type="GeneID" id="10642866"/>
    <dbReference type="KEGG" id="mig:Metig_0031"/>
    <dbReference type="HOGENOM" id="CLU_045401_2_2_2"/>
    <dbReference type="OMA" id="ASCAEYI"/>
    <dbReference type="OrthoDB" id="69007at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004459">
        <property type="term" value="F:L-lactate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046554">
        <property type="term" value="F:malate dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019752">
        <property type="term" value="P:carboxylic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001557">
        <property type="entry name" value="L-lactate/malate_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018177">
        <property type="entry name" value="L-lactate_DH_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022383">
        <property type="entry name" value="Lactate/malate_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001236">
        <property type="entry name" value="Lactate/malate_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015955">
        <property type="entry name" value="Lactate_DH/Glyco_Ohase_4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02866">
        <property type="entry name" value="Ldh_1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00056">
        <property type="entry name" value="Ldh_1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000102">
        <property type="entry name" value="Lac_mal_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00086">
        <property type="entry name" value="LLDHDRGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56327">
        <property type="entry name" value="SSF56327"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00064">
        <property type="entry name" value="L_LDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="5 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ldh_1_N" evidence="6">
        <location>
            <begin position="1"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="domain" description="Ldh_1_C" evidence="7">
        <location>
            <begin position="148"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="3">
        <location>
            <position position="178"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="92"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="123"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="154"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR000102-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR000102-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEF95592.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="313" mass="34502" checksum="A0B7F469220110F9" modified="2011-07-27" version="1">MKVSVIGASGRIGSSVSFLLAKEPDIKDLIMISREKSLNKLKGLQMDIYDALAGLRSDANIEVHSDKDLSVVDGSDIVIITAGVPRKENMSRLDLAKINAEIVGRYAREIAKICDTKLFIITNPVDVMTYKAFIESNYDRNMVFGLGTHLDSLRFKVAIAKFFGVHIDDVRTRIIGEHGDTMVPLLSATGIGGIPIQMMPNFEKFPYKEVIDDVKNKGSEIIKLNGGSEFGPASAVINIVNCIIHDEKRLLTLSAYLDGEIDGIRDVCIGVPVKVGRNGIEEVVPIKLENDELEAFRHSVKVVKEYCELVKNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>F6BE63</accession>
    <name>F6BE63_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Coenzyme F420 hydrogenase, subunit gamma</fullName>
            <ecNumber evidence="4">1.12.98.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metig_1202</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FrhG family.</text>
    </comment>
    <dbReference type="EC" id="1.12.98.1" evidence="4"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96740.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799339.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1202"/>
    <dbReference type="EnsemblBacteria" id="AEF96740">
        <property type="protein sequence ID" value="AEF96740"/>
        <property type="gene ID" value="Metig_1202"/>
    </dbReference>
    <dbReference type="GeneID" id="10644062"/>
    <dbReference type="KEGG" id="mig:Metig_1202"/>
    <dbReference type="HOGENOM" id="CLU_075477_0_0_2"/>
    <dbReference type="OMA" id="HESFVPI"/>
    <dbReference type="OrthoDB" id="54703at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050454">
        <property type="term" value="F:coenzyme F420 hydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016151">
        <property type="term" value="F:nickel cation binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.700">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017681">
        <property type="entry name" value="Coenz_F420_hydrogenase_gsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006137">
        <property type="entry name" value="NADH_UbQ_OxRdtase-like_20kDa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037024">
        <property type="entry name" value="NiFe_Hase_small_N_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00037">
        <property type="entry name" value="Fer4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01058">
        <property type="entry name" value="Oxidored_q6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03294">
        <property type="entry name" value="FrhG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="3">
        <location>
            <begin position="180"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="3">
        <location>
            <begin position="210"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEF96740.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="241" mass="26139" checksum="706E4B26A983C3A4" modified="2011-07-27" version="1">MVKIAHIHMCGCTGCLISLADTYEQLLDILNKVELVYALTLVDEKTEIKEIDDKIIIERKIPDDIDIALVEGSVCLDDEHSLKEILEVREKSKIVVALGACAATGGVTRFCRGNQMSKPVHSSFVPIGEVIKVDLALPGCPPSTESIVKLIGAALNGDTVYLEPYAELANKTEVCGCDLLYKVVNKSLCMGCGTCAAACPTRAIEMLDGRPNVLKELCIKCGACSVQCPRIRFPKLIEEIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>F6BE70</accession>
    <name>F6BE70_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_1209</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96747.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799346.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1209"/>
    <dbReference type="EnsemblBacteria" id="AEF96747">
        <property type="protein sequence ID" value="AEF96747"/>
        <property type="gene ID" value="Metig_1209"/>
    </dbReference>
    <dbReference type="GeneID" id="10644069"/>
    <dbReference type="KEGG" id="mig:Metig_1209"/>
    <dbReference type="HOGENOM" id="CLU_120866_1_0_2"/>
    <dbReference type="OMA" id="PCPELIY"/>
    <dbReference type="OrthoDB" id="88086at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF96747.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="165" mass="19051" checksum="827BD255918B95B7" modified="2011-07-27" version="1">MKGKKIAIVAHCILNQNSVVNGLERAEGAFNDVVELLLKKNYAIIQLPCPEMLYLGIDRVGKTKEEYDTEEYRELCREILKPIVKYLGEYSKEGFRFILIGIEGSPTCGIFKTVTKEGLIDGRGILMEEFLKMLENEHIDLKKIDFPVNMNEYNEFLKELERLLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEA3</accession>
    <name>F6BEA3_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Tetrahydromethanopterin S-methyltransferase subunit A</fullName>
            <ecNumber evidence="13">2.1.1.86</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">N5-methyltetrahydromethanopterin--coenzyme M methyltransferase subunit A</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">mtrA</name>
        <name evidence="15" type="ordered locus">Metig_1243</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 13">Part of a complex that catalyzes the formation of methyl-coenzyme M and tetrahydromethanopterin from coenzyme M and methyl-tetrahydromethanopterin. This is an energy-conserving, sodium-ion translocating step.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 13">
            <text>5-methyl-5,6,7,8-tetrahydromethanopterin + coenzyme M + 2 Na(+)(in) = 5,6,7,8-tetrahydromethanopterin + methyl-coenzyme M + 2 Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:53492"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:58103"/>
            <dbReference type="ChEBI" id="CHEBI:58116"/>
            <dbReference type="ChEBI" id="CHEBI:58286"/>
            <dbReference type="ChEBI" id="CHEBI:58319"/>
            <dbReference type="EC" id="2.1.1.86"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>5-hydroxybenzimidazolylcob(I)amide</name>
            <dbReference type="ChEBI" id="CHEBI:60494"/>
        </cofactor>
        <text evidence="13">Binds 1 5-hydroxybenzimidazolylcobamide group.</text>
    </comment>
    <comment type="pathway">
        <text evidence="13">One-carbon metabolism; methanogenesis from CO(2); methyl-coenzyme M from 5,10-methylene-5,6,7,8-tetrahydromethanopterin: step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5 13">The complex is composed of 8 subunits; MtrA, MtrB, MtrC, MtrD, MtrE, MtrF, MtrG and MtrH.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3 13">Cell membrane</location>
            <topology evidence="3 13">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Membrane</location>
            <topology evidence="4">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the MtrA family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.86" evidence="13"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96780.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799379.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1243"/>
    <dbReference type="EnsemblBacteria" id="AEF96780">
        <property type="protein sequence ID" value="AEF96780"/>
        <property type="gene ID" value="Metig_1243"/>
    </dbReference>
    <dbReference type="GeneID" id="10644106"/>
    <dbReference type="KEGG" id="mig:Metig_1243"/>
    <dbReference type="HOGENOM" id="CLU_100863_0_0_2"/>
    <dbReference type="OMA" id="ARMKIVS"/>
    <dbReference type="OrthoDB" id="89197at2157"/>
    <dbReference type="UniPathway" id="UPA00640">
        <property type="reaction ID" value="UER00698"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050897">
        <property type="term" value="F:cobalt ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030269">
        <property type="term" value="F:tetrahydromethanopterin S-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019386">
        <property type="term" value="P:methanogenesis, from carbon dioxide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006814">
        <property type="term" value="P:sodium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01093">
        <property type="entry name" value="MtrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013347">
        <property type="entry name" value="MeTrfase_F_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030688">
        <property type="entry name" value="MeTrfase_MtrA/MtxA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005778">
        <property type="entry name" value="MtrA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04208">
        <property type="entry name" value="MtrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09472">
        <property type="entry name" value="MtrF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF500207">
        <property type="entry name" value="MtrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF009452">
        <property type="entry name" value="MtrA_MtxA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01111">
        <property type="entry name" value="mtrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02507">
        <property type="entry name" value="MtrF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="13" id="KW-0170">Cobalt</keyword>
    <keyword evidence="12 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="11 13" id="KW-0484">Methanogenesis</keyword>
    <keyword evidence="7 13 15" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="6 13" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 13 15" id="KW-0808">Transferase</keyword>
    <keyword evidence="9 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="231"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="domain" description="MtrF" evidence="14">
        <location>
            <begin position="187"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="85"/>
        </location>
        <ligand>
            <name>5-hydroxybenzimidazolylcob(I)amide</name>
            <dbReference type="ChEBI" id="CHEBI:60494"/>
            <note>cofactor</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF09472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="AEF96780.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="253" mass="26303" checksum="0390FCA738A0570E" modified="2011-07-27" version="1">MANKKAPAAGWPIVSGEYVVGNPESCVGVVTLGSHGLEQACIDAGAAIAGPCHTENLGIEKVVANYISNPNIRFMVLCGSEVQGHITGQCFKALWENGIGDDGGIIGAKGAIPFLENVGKDAVERFQRQIVEVVDLIDCEDVSKITAAIKECISKDPGAIDEEPMVLDLEGGGAGGEEAEGGFEIEGIPTVVEPDLEACQKLLEKLEYKVGLMTRDIGLASGVSSESVKGFAIGSLLAVVLVGIPIILKFLIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEC1</accession>
    <name>F6BEC1_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ribonuclease J</fullName>
            <shortName evidence="8">RNase J</shortName>
            <ecNumber evidence="8">3.1.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">rnj</name>
        <name evidence="10" type="ordered locus">Metig_1261</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">An RNase that has 5'-3' exonuclease activity. May be involved in RNA degradation.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="8">Binds 2 Zn(2+) ions per subunit. It is not clear if Zn(2+) or Mg(2+) is physiologically important.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the metallo-beta-lactamase superfamily. RNA-metabolizing metallo-beta-lactamase-like family. Archaeal RNase J subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="8"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96798.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1261"/>
    <dbReference type="EnsemblBacteria" id="AEF96798">
        <property type="protein sequence ID" value="AEF96798"/>
        <property type="gene ID" value="Metig_1261"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_1261"/>
    <dbReference type="HOGENOM" id="CLU_008727_4_1_2"/>
    <dbReference type="OMA" id="KNTCVFE"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004534">
        <property type="term" value="F:5'-3' exoribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006401">
        <property type="term" value="P:RNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10710">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01492">
        <property type="entry name" value="RNase_J_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011108">
        <property type="entry name" value="RMMBL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004613">
        <property type="entry name" value="RNase_J"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042173">
        <property type="entry name" value="RNase_J_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030879">
        <property type="entry name" value="RNase_J_arc"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07521">
        <property type="entry name" value="RMMBL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="SSF56281"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00649">
        <property type="entry name" value="MG423"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 8" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="4 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 8" id="KW-0540">Nuclease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 8" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5 8" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Lactamase_B" evidence="9">
        <location>
            <begin position="25"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="8">
        <location>
            <begin position="393"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="91"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="93"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="95"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="96"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="161"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="183"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="419"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AEF96798.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="458" mass="50928" checksum="E2175D5481CB1DDA" modified="2011-07-27" version="1">MPIKHEGCDNLQLEVIAIGGYEEVGRNMTAVNVDGEIVIFDMGIRLDRVMIHEDTDISKMHSLNLIEMGVIPNDTVMKHVEGEVKAIVLTHGHLDHIGAVTKLAHRYNAPIIGTPYTLELVKREILSEKKFDVRNPLVVLNPGETKDLTPNLSLEFIRATHSIPDSVFAALHTPYGTILYANDFKFDNFPVVGERPDYRALKRLGKNGVICMISESTRVDHEGKTPSEIIAANLLKNDLLGADNDGKGVVVTTFSSHIARIKSITEIAVKMDRIPVLLGRSMMKYCGIAQDIGIVEFPEGTRMYGDPSAIDSALRTIAKEGKENYLLIVTGHQGEEGAVLSRMATNKTPYQFEKHDQVVFSADPIPNPINAAQRYMLESRLKLLGVRVFKGAHVSGHAAKEDHRDMIRWINAEHIIPSHGDFNLTAAYTKLAEEEGYRLGEDVHLLRNGQCLSFERII</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEC5</accession>
    <name>F6BEC5_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KAP P-loop domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1265</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96802.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799400.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1265"/>
    <dbReference type="EnsemblBacteria" id="AEF96802">
        <property type="protein sequence ID" value="AEF96802"/>
        <property type="gene ID" value="Metig_1265"/>
    </dbReference>
    <dbReference type="GeneID" id="10644128"/>
    <dbReference type="KEGG" id="mig:Metig_1265"/>
    <dbReference type="HOGENOM" id="CLU_539285_0_0_2"/>
    <dbReference type="OrthoDB" id="87610at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011646">
        <property type="entry name" value="KAP_P-loop"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07693">
        <property type="entry name" value="KAP_NTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="KAP NTPase" evidence="1">
        <location>
            <begin position="40"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96802.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="516" mass="60891" checksum="B0FD2448D0BC1BCE" modified="2011-07-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>F6BED9</accession>
    <name>F6BED9_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 8">Serine hydroxymethyltransferase</fullName>
            <shortName evidence="8">SHMT</shortName>
            <shortName evidence="8">Serine methylase</shortName>
            <ecNumber evidence="8">2.1.2.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">glyA</name>
        <name evidence="11" type="ordered locus">Metig_0039</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the reversible interconversion of serine and glycine with tetrahydromethanopterin (H4MPT) serving as the one-carbon carrier. Also exhibits a pteridine-independent aldolase activity toward beta-hydroxyamino acids, producing glycine and aldehydes, via a retro-aldol mechanism.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>5,10-methylenetetrahydromethanopterin + glycine + H2O = 5,6,7,8-tetrahydromethanopterin + L-serine</text>
            <dbReference type="Rhea" id="RHEA:47104"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:57818"/>
            <dbReference type="ChEBI" id="CHEBI:58103"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 8 9">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2 8">Amino-acid biosynthesis; glycine biosynthesis; glycine from L-serine: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the SHMT family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.1.2.-" evidence="8"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95600.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798209.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0039"/>
    <dbReference type="EnsemblBacteria" id="AEF95600">
        <property type="protein sequence ID" value="AEF95600"/>
        <property type="gene ID" value="Metig_0039"/>
    </dbReference>
    <dbReference type="GeneID" id="10642874"/>
    <dbReference type="KEGG" id="mig:Metig_0039"/>
    <dbReference type="HOGENOM" id="CLU_022477_2_1_2"/>
    <dbReference type="OMA" id="SHPAGLI"/>
    <dbReference type="OrthoDB" id="22518at2157"/>
    <dbReference type="UniPathway" id="UPA00288">
        <property type="reaction ID" value="UER01023"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004372">
        <property type="term" value="F:glycine hydroxymethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019264">
        <property type="term" value="P:glycine biosynthetic process from serine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035999">
        <property type="term" value="P:tetrahydrofolate interconversion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00378">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00051">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001085">
        <property type="entry name" value="Ser_HO-MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019798">
        <property type="entry name" value="Ser_HO-MeTrfase_PLP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039429">
        <property type="entry name" value="SHMT-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11680">
        <property type="entry name" value="PTHR11680"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00464">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000412">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00096">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="5 8" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="7 8 9" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 8 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SHMT" evidence="10">
        <location>
            <begin position="10"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="8">
        <location>
            <begin position="120"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="30"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="50"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="52"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="60"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="171"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="199"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="225"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="363"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="8 9">
        <location>
            <position position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00016846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000412-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AEF95600.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="428" mass="47685" checksum="228529D3DFB7768F" modified="2011-07-27" version="1">MNPSEVPGYVRDVLMKHHNWMRESIKLIASENIASRAVREACASDFAHRYAEGLPGKRLYQGCKYIDMVEELAIELAKDVFNAEHANVQPTSGVVANLAVFFAETKPGDTLMAMDVPNGGHISHWKVSAAGIRGLKIVPHPFDEKEMNIDVDKMIKEIYEHKPRLILFGGSLFLFPHPVKEAVDAAKEIGATVAYDGAHVLGLIAGKQFQDPLREGADYLMGSTHKTFFGPQGGIILTKKEYAEKIDEKVFPGVVSNHHLHHKAGLAIALAEAKEFGEAYAKQVIKNAKALAQALYERGFNVLCEDKGFTESHQVVLDIAGSKDIPFSASELAKMYEDANIILNKNLLPWDDVNKSDDPSGIRLGTQECTRLGMKESEMEEIAEFMKRIAIDKEKPEKVREDVKEFAREYSTVHYSFDGGDAFEYIKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEF9</accession>
    <name>F6BEF9_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">FeS_assembly_P domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0059</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95620.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798229.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0059"/>
    <dbReference type="EnsemblBacteria" id="AEF95620">
        <property type="protein sequence ID" value="AEF95620"/>
        <property type="gene ID" value="Metig_0059"/>
    </dbReference>
    <dbReference type="GeneID" id="10642894"/>
    <dbReference type="KEGG" id="mig:Metig_0059"/>
    <dbReference type="HOGENOM" id="CLU_091588_3_0_2"/>
    <dbReference type="OMA" id="TVADPHM"/>
    <dbReference type="OrthoDB" id="113911at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034904">
        <property type="entry name" value="FSCA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002744">
        <property type="entry name" value="MIP18-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01883">
        <property type="entry name" value="FeS_assembly_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117916">
        <property type="entry name" value="SSF117916"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FeS_assembly_P" evidence="1">
        <location>
            <begin position="4"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF95620.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="94" mass="10302" checksum="2304E2E84DE1EAE1" modified="2011-07-27" version="1">MVSKEDIMNALKKVTDPHMGISIVDMGLISDVEVDDEGNVKFTLTPTNPACMSVLGMAMHAKEVVKGIEGVKSVKVEVKGHMMEKEINEMLNKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEG9</accession>
    <name>F6BEG9_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Alpha-glucan phosphorylase</fullName>
            <ecNumber evidence="2">2.4.1.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0069</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycogen phosphorylase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.1" evidence="2"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95630.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798239.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0069"/>
    <dbReference type="EnsemblBacteria" id="AEF95630">
        <property type="protein sequence ID" value="AEF95630"/>
        <property type="gene ID" value="Metig_0069"/>
    </dbReference>
    <dbReference type="GeneID" id="10642904"/>
    <dbReference type="KEGG" id="mig:Metig_0069"/>
    <dbReference type="HOGENOM" id="CLU_015112_1_0_2"/>
    <dbReference type="OMA" id="LYRHGYF"/>
    <dbReference type="OrthoDB" id="19755at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008184">
        <property type="term" value="F:glycogen phosphorylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102250">
        <property type="term" value="F:linear malto-oligosaccharide phosphorylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102499">
        <property type="term" value="F:SHG alpha-glucan phosphorylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011834">
        <property type="entry name" value="Agluc_phsphrylas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000811">
        <property type="entry name" value="Glyco_trans_35"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00343">
        <property type="entry name" value="Phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02094">
        <property type="entry name" value="more_P_ylases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF95630.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="519" mass="60112" checksum="5EF79375F5CB5256" modified="2011-07-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEI9</accession>
    <name>F6BEI9_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Protein tyrosine phosphatase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0089</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95650.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798259.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0089"/>
    <dbReference type="EnsemblBacteria" id="AEF95650">
        <property type="protein sequence ID" value="AEF95650"/>
        <property type="gene ID" value="Metig_0089"/>
    </dbReference>
    <dbReference type="GeneID" id="10642924"/>
    <dbReference type="KEGG" id="mig:Metig_0089"/>
    <dbReference type="HOGENOM" id="CLU_071415_3_3_2"/>
    <dbReference type="OMA" id="EKIAFVC"/>
    <dbReference type="OrthoDB" id="108917at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023485">
        <property type="entry name" value="Ptyr_pPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036196">
        <property type="entry name" value="Ptyr_pPase_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01451">
        <property type="entry name" value="LMWPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00226">
        <property type="entry name" value="LMWPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52788">
        <property type="entry name" value="SSF52788"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="LMWPc" evidence="1">
        <location>
            <begin position="1"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF95650.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="128" mass="14552" checksum="1FB7B3F91687B2F1" modified="2011-07-27" version="1">MKVLFVCVHNKRRSIMAEAFGKKYGLNAYSAGLEKTDGVDEKVVEVLKEKNLNAKQKPQTIDEVVREVGNFDVVVTMGCLDKCPFVPAKKHISWNIEDPAGKDIEVYRKIRDEIEEKVKELTNSLKDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEJ0</accession>
    <name>F6BEJ0_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">TatD-related deoxyribonuclease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0090</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the metallo-dependent hydrolases superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95651.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF95651">
        <property type="protein sequence ID" value="AEF95651"/>
        <property type="gene ID" value="Metig_0090"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0090"/>
    <dbReference type="HOGENOM" id="CLU_061552_1_0_2"/>
    <dbReference type="OMA" id="HPMGYPK"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018228">
        <property type="entry name" value="DNase_TatD-rel_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001130">
        <property type="entry name" value="TatD-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012022">
        <property type="entry name" value="UCP005295"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42658">
        <property type="entry name" value="PTHR42658"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01026">
        <property type="entry name" value="TatD_DNase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005295">
        <property type="entry name" value="UCP005295_TatD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="SSF51556"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01137">
        <property type="entry name" value="TATD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRNR" id="PIRNR005295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF95651.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="252" mass="29132" checksum="900CD7E3D8FD90AC" modified="2011-07-27" version="1">MMIDAHTHLDVRSFEDLEKMALSGIETIITCSHDPYKMSVPEVYLDHWDRLINLEVKRGKMAGVEVKVALGVHPMGYPKNWEILIKKLPEFLENENVVAIGETGLHYLTEDEKNLLREQLILAKDYNMPIIVHTPEKNKKEALIEILKILDEVKIKDDLVMIDHINKETVDLIDRDVYVGLTVQPSMKISHEEAAEIIKNYDKKFILSSDLGSLKADIYALPRTKLYMKKIGVEEEKIINSIYKNAKEFYRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-06-02" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEJ4</accession>
    <name>F6BEJ4_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Sulfur relay protein TusB/DsrH</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_0094</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95655.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798264.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF95655">
        <property type="protein sequence ID" value="AEF95655"/>
        <property type="gene ID" value="Metig_0094"/>
    </dbReference>
    <dbReference type="GeneID" id="10642929"/>
    <dbReference type="KEGG" id="mig:Metig_0094"/>
    <dbReference type="HOGENOM" id="CLU_2366172_0_0_2"/>
    <dbReference type="OMA" id="SPFECNA"/>
    <dbReference type="OrthoDB" id="108769at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002143">
        <property type="term" value="P:tRNA wobble position uridine thiolation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027396">
        <property type="entry name" value="DsrEFH-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007215">
        <property type="entry name" value="Sulphur_relay_TusB/DsrH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37526">
        <property type="entry name" value="PTHR37526"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04077">
        <property type="entry name" value="DsrH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75169">
        <property type="entry name" value="SSF75169"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03011">
        <property type="entry name" value="sulf_tusB_dsrH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF95655.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="95" mass="10887" checksum="E3DA330E110E92DC" modified="2011-07-27" version="1">MVLFILLKSPFECNAIELMERLRDGNDGVLLAEDAIYYATVSKVRDELLKKGYKIYAMQDDVLARGFDSIENVELIDYETAVDLIMEKYSKVISL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEN6</accession>
    <name>F6BEN6_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Methylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_1296</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96833.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799430.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1296"/>
    <dbReference type="EnsemblBacteria" id="AEF96833">
        <property type="protein sequence ID" value="AEF96833"/>
        <property type="gene ID" value="Metig_1296"/>
    </dbReference>
    <dbReference type="GeneID" id="10644159"/>
    <dbReference type="KEGG" id="mig:Metig_1296"/>
    <dbReference type="HOGENOM" id="CLU_018398_6_2_2"/>
    <dbReference type="OMA" id="EWDDWME"/>
    <dbReference type="OrthoDB" id="72076at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004557">
        <property type="entry name" value="PrmC-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00537">
        <property type="entry name" value="hemK_rel_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF96833.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="202" mass="22805" checksum="A628DE0EA54AB792" modified="2011-07-27" version="1">MKTIKIGDIIIKTHPQVYEPAEDSELLMENLVDVKNKVVLDVGTGTGIQAINAAKKGAKIVVGVDINPYAIQIAKENAILNNLELNKKIFFFESDLFENVEGKFDVILFNAPYLPTSDEERLEGWLNYAFDGGEDGREVLDRFIEEVGDYLKENGIVQILQSSLTGEEKTIKMLNKQGFKAKKTAYKKFPFEELQVITAWRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-06-02" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEN8</accession>
    <name>F6BEN8_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1298</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96835.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799432.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96835">
        <property type="protein sequence ID" value="AEF96835"/>
        <property type="gene ID" value="Metig_1298"/>
    </dbReference>
    <dbReference type="GeneID" id="10644161"/>
    <dbReference type="KEGG" id="mig:Metig_1298"/>
    <dbReference type="HOGENOM" id="CLU_195001_0_0_2"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="49"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96835.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="79" mass="8576" checksum="1543556F5F2A1D78" modified="2011-07-27" version="1">MDKELTEKLAKISSARKKRTLLGAILVSLSLILTQIAILILIGVIDLGIVFAVMLIIVSPLFLAIGLYLILHTPPIVLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-06-02" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEP2</accession>
    <name>F6BEP2_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1302</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96839.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799436.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96839">
        <property type="protein sequence ID" value="AEF96839"/>
        <property type="gene ID" value="Metig_1302"/>
    </dbReference>
    <dbReference type="GeneID" id="10644165"/>
    <dbReference type="KEGG" id="mig:Metig_1302"/>
    <dbReference type="HOGENOM" id="CLU_1187728_0_0_2"/>
    <dbReference type="OMA" id="EIWEPID"/>
    <dbReference type="OrthoDB" id="94042at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="213"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96839.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="246" mass="28256" checksum="D72CF4A46CC5510A" modified="2011-07-27" version="1">MIKIKYLLILLLLSIGVSQSAYADVTKISYYNMSVNVDSNPSHVVNTITIQNLVKYPLVLGIGELRLQKQTPKKLWIIPIPFTESKEPIKVKNLKGYYYYGGTKTDMDTKVKYFDNYTVIYYEIWEPIDPKGNITLVIEYDAEDIVDNGLLFKTVSIPVGCDMEIDNFNINFKSSEYKTYQNPADKNMHIPKNTLFMINAEFSILPFPELPTYGYVLFWLTILTILVLIFAYYEIKGMGGSKKGDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEP8</accession>
    <name>F6BEP8_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative KaiC associated regulatory domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_1308</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96845.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799442.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1308"/>
    <dbReference type="EnsemblBacteria" id="AEF96845">
        <property type="protein sequence ID" value="AEF96845"/>
        <property type="gene ID" value="Metig_1308"/>
    </dbReference>
    <dbReference type="GeneID" id="10644171"/>
    <dbReference type="KEGG" id="mig:Metig_1308"/>
    <dbReference type="HOGENOM" id="CLU_109226_0_0_2"/>
    <dbReference type="OMA" id="TRVTWVD"/>
    <dbReference type="OrthoDB" id="105306at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022285">
        <property type="entry name" value="CHP03879_regulat_dom_put"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013196">
        <property type="entry name" value="HTH_11"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08279">
        <property type="entry name" value="HTH_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03879">
        <property type="entry name" value="near_KaiC_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH_11" evidence="2">
        <location>
            <begin position="57"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="107"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF96845.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="156" mass="17655" checksum="DE72E880A9844BF2" modified="2011-07-27" version="1">MEVPIVPKSRREIHQLETMLLLGTLLQPKVLEIIKDPVEKLTWVDSLAVASGALAREKAGMTVSEIAEELGRTEQTIRKHLKGETKAGEFVKETYELLKKGELKLGDLEAQVELISAEEKIKKLEEEVKELEEQKKTLEENLGKVKDTLKKLLDEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>F6BER2</accession>
    <name>F6BER2_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ribose-5-phosphate isomerase A</fullName>
            <ecNumber evidence="2">5.3.1.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">Phosphoriboisomerase A</fullName>
            <shortName evidence="2">PRI</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2" type="primary">rpiA</name>
        <name evidence="3" type="ordered locus">Metig_1322</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the reversible conversion of ribose-5-phosphate to ribulose 5-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>aldehydo-D-ribose 5-phosphate = D-ribulose 5-phosphate</text>
            <dbReference type="Rhea" id="RHEA:14657"/>
            <dbReference type="ChEBI" id="CHEBI:58121"/>
            <dbReference type="ChEBI" id="CHEBI:58273"/>
            <dbReference type="EC" id="5.3.1.6"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Carbohydrate degradation; pentose phosphate pathway; D-ribose 5-phosphate from D-ribulose 5-phosphate (non-oxidative stage): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ribose 5-phosphate isomerase family.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.6" evidence="2"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96859.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799456.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1322"/>
    <dbReference type="EnsemblBacteria" id="AEF96859">
        <property type="protein sequence ID" value="AEF96859"/>
        <property type="gene ID" value="Metig_1322"/>
    </dbReference>
    <dbReference type="GeneID" id="10644185"/>
    <dbReference type="KEGG" id="mig:Metig_1322"/>
    <dbReference type="HOGENOM" id="CLU_056590_1_1_2"/>
    <dbReference type="OMA" id="YDWDEVN"/>
    <dbReference type="OrthoDB" id="98657at2157"/>
    <dbReference type="UniPathway" id="UPA00115">
        <property type="reaction ID" value="UER00412"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004751">
        <property type="term" value="F:ribose-5-phosphate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009052">
        <property type="term" value="P:pentose-phosphate shunt, non-oxidative branch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01398">
        <property type="entry name" value="RPI_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00170">
        <property type="entry name" value="Rib_5P_isom_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020672">
        <property type="entry name" value="Ribose5P_isomerase_typA_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004788">
        <property type="entry name" value="Ribose5P_isomerase_type_A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11934">
        <property type="entry name" value="PTHR11934"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06026">
        <property type="entry name" value="Rib_5-P_isom_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="SSF100950"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00021">
        <property type="entry name" value="rpiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 2 3" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Substrate binding" evidence="2">
        <location>
            <begin position="26"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="2">
        <location>
            <begin position="81"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="2">
        <location>
            <begin position="95"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="2">
        <location>
            <position position="104"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="122"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF96859.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="231" mass="25072" checksum="A8DC88511B98C1E0" modified="2011-07-27" version="1">MENAKSLVAKEAAKLIEDGMVVGLGSGSTAGLFIMELGKRIQEEELTVFGVPTSFEARMLALQYGVPLVSLDEYDVDIAVDGADEVEKDTLYLIKGGGGCHTQEKIVDYSAKEFVVIVDESKLVNKLGEKYPIPVEVVPFAYSVVIKELINMGGNPTIRLGERKRGPIITDNGNMIIDVSMDIDNAEKLEKELNSIPGVVENGIFTKVDKVLVGTAKGVKTLKKKKKKGKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>F6BER7</accession>
    <name>F6BER7_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 10">Shikimate kinase</fullName>
            <shortName evidence="10">SK</shortName>
            <ecNumber evidence="5 10">2.7.1.71</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">aroK</name>
        <name evidence="12" type="ordered locus">Metig_1327</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>ATP + shikimate = 3-phosphoshikimate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13121"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:36208"/>
            <dbReference type="ChEBI" id="CHEBI:145989"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.71"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 10">Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 5/7.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 10">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 10">Belongs to the GHMP kinase family. Archaeal shikimate kinase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.71" evidence="5 10"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96864.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1327"/>
    <dbReference type="EnsemblBacteria" id="AEF96864">
        <property type="protein sequence ID" value="AEF96864"/>
        <property type="gene ID" value="Metig_1327"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_1327"/>
    <dbReference type="HOGENOM" id="CLU_073768_0_0_2"/>
    <dbReference type="OMA" id="WDVLVWT"/>
    <dbReference type="UniPathway" id="UPA00053">
        <property type="reaction ID" value="UER00088"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004765">
        <property type="term" value="F:shikimate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009073">
        <property type="term" value="P:aromatic amino acid family biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:cellular amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009423">
        <property type="term" value="P:chorismate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.890">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00370">
        <property type="entry name" value="Shik_kinase_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036554">
        <property type="entry name" value="GHMP_kinase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006204">
        <property type="entry name" value="GHMP_kinase_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010189">
        <property type="entry name" value="SK_arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20861:SF3">
        <property type="entry name" value="PTHR20861:SF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00288">
        <property type="entry name" value="GHMP_kinases_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005758">
        <property type="entry name" value="Shikimt_kin_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55060">
        <property type="entry name" value="SSF55060"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01920">
        <property type="entry name" value="Shik_kin_archae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="9 10" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="8 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10 12" id="KW-0418">Kinase</keyword>
    <keyword evidence="7 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 12" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GHMP_kinases_N" evidence="11">
        <location>
            <begin position="81"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="88"/>
            <end position="98"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AEF96864.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="285" mass="30822" checksum="EF5FC4E29F31654C" modified="2011-07-27" version="1">MSMKATAIASASGTIINAIATGKGSAFGINLKVKATVELFDDGKNKIEGIVKDNPKVKPNLIKRCVKNVLDYFGLNYSAKVETETQIPIKSGLSSSSATSNAVVLATFAALGEKIDDELVLDLGIKSSFDEKLTVTGAYDDATASYYGGITITDNIERKILKRDKFKDDLKVLVLIPNLKKNVDVNRMKLLKDYVEVAFNECLNGNYYRALFLNGILYASALNFPTNIAIEAIENNAVTAGLSGTGPSYIALCYEEDVEKVKKTLEKYGRVISSELDYGGAEVIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEW1</accession>
    <name>F6BEW1_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Aminotransferase</fullName>
            <ecNumber evidence="1">2.6.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_0137</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.-" evidence="1"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95697.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798306.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0137"/>
    <dbReference type="EnsemblBacteria" id="AEF95697">
        <property type="protein sequence ID" value="AEF95697"/>
        <property type="gene ID" value="Metig_0137"/>
    </dbReference>
    <dbReference type="GeneID" id="10642972"/>
    <dbReference type="KEGG" id="mig:Metig_0137"/>
    <dbReference type="HOGENOM" id="CLU_017584_4_3_2"/>
    <dbReference type="OMA" id="WIVADEV"/>
    <dbReference type="OrthoDB" id="32104at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009058">
        <property type="term" value="P:biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004838">
        <property type="entry name" value="NHTrfase_class1_PyrdxlP-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00105">
        <property type="entry name" value="AA_TRANSFER_CLASS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotran_1_2" evidence="2">
        <location>
            <begin position="30"/>
            <end position="362"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF95697.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="370" mass="42200" checksum="CE806EDA3AF98138" modified="2011-07-27" version="1">MLSERVINFESFEVMDILAKCEEMERRGEDVIHLEIGEPDFDTPKPIVDACLNALKDGKTHYTDSLGIYDLREKISEEYKGEYGVDVSPNNIIITGGSSLGLFFAISSIIDKGDKVLIQNPSYPCYRNIIKFCGGVPVYCNFEDIESYISKRTKAVIINSPSNPLGEVISREIFEEIYENIPYVISDEIYSGLIYEGKHISAIEFDENLEKTIVVNGFSKLYAMTGWRIGYVISNKEIINAILKLQQNLFIAPTTFVQYGALKVFDEDVKKARDEMIKEFDRRRRLVLKYVKSFGWEVNNPIGAYYVFPNIGMDGREFSTKLLEEKKVATVPGIAFGSAGKNHIRISYANSYEKIKEGMERIEEFIENNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>F6BF28</accession>
    <name>F6BF28_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_1363</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96898.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799495.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1363"/>
    <dbReference type="EnsemblBacteria" id="AEF96898">
        <property type="protein sequence ID" value="AEF96898"/>
        <property type="gene ID" value="Metig_1363"/>
    </dbReference>
    <dbReference type="GeneID" id="10644227"/>
    <dbReference type="KEGG" id="mig:Metig_1363"/>
    <dbReference type="HOGENOM" id="CLU_081185_0_0_2"/>
    <dbReference type="OMA" id="MKYGITE"/>
    <dbReference type="OrthoDB" id="66792at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009573">
        <property type="entry name" value="HcgC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06690">
        <property type="entry name" value="HcgC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF96898.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="259" mass="29836" checksum="31C2D0A8C0CBE519" modified="2011-07-27" version="1">MKYGITERVKTIDTKIKINDIIKHIVEKKAKAIKYFLEGEEFRQTVIFGAYLCGGYIAHSITDYSEEVILVDIQQHLKEFVFNKNVKFMEMNQFKLKMRTGEVNPDLVIDITGIGGISPDFLQKFNPKCLIVEDPKGCYDEYIYKADNTMERATVGEKIGILKTYRKSRISKTSGTMTLTIDTVMDSAREVLDLDGVLYVIPNLRYYEGIIFHEKNIHKFLDEVHMPALTISALEDVEEEANKILENNLSRIKSFVEKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>F6BF60</accession>
    <name>F6BF60_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">5-methylcytosine restriction system component-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_1395</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96930.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799526.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1395"/>
    <dbReference type="REBASE" id="36260">
        <property type="entry name" value="Mig5McrBCP"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96930">
        <property type="protein sequence ID" value="AEF96930"/>
        <property type="gene ID" value="Metig_1395"/>
    </dbReference>
    <dbReference type="GeneID" id="10644268"/>
    <dbReference type="KEGG" id="mig:Metig_1395"/>
    <dbReference type="HOGENOM" id="CLU_048696_1_2_2"/>
    <dbReference type="OMA" id="PWCELIL"/>
    <dbReference type="OrthoDB" id="69722at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019292">
        <property type="entry name" value="McrC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38733">
        <property type="entry name" value="PTHR38733"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10117">
        <property type="entry name" value="McrBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF96930.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="407" mass="49037" checksum="D85B3BA5EABDB48A" modified="2011-07-27" version="1">MNLITFYEYQEKDIEELKNELNLNEKEEIFNLFKKINGGSEDKIFTLYHNKIKANNYVGFASIKDVSIQILPKIFKNSEEKEYLFLNMLNFAFDLKLEEKEVRAINSLSKAFYEIFIYLFAKSLLEEIKRGIYKEYIKIRGEEKFLKGKLLIEKEIRKLPHQRDRFFVEYFLFDENNLLNQIFYYAVATSLRKTKIRTNKKYLSELMLIFDGIELKKINVEDFKKVKFTRLNNRFKKSFNLAKIVLTSLGELRGEDNVGFFIDMNELFERFICNILKRKFSIGYQEEFNLLKETKYLDKIKQKPDFIVYKNGEVKLVLDAKYKEIEENLSPDILRQIYTYAKKYKVPSALIFPKFKNYNNFKTNVDYCKFFDETELYLLVYNLETLKNNEMDEEFLNAIDKLLNKFW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F6BF61</accession>
    <name>F6BF61_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ATPase associated with various cellular activities AAA_5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1396</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96931.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1396"/>
    <dbReference type="REBASE" id="36260">
        <property type="entry name" value="Mig5McrBCP"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96931">
        <property type="protein sequence ID" value="AEF96931"/>
        <property type="gene ID" value="Metig_1396"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_1396"/>
    <dbReference type="HOGENOM" id="CLU_405789_0_0_2"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011704">
        <property type="entry name" value="ATPase_dyneun-rel_AAA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07728">
        <property type="entry name" value="AAA_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA_5" evidence="1">
        <location>
            <begin position="472"/>
            <end position="558"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96931.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="677" mass="78676" checksum="7F2F9410D31A7DF9" modified="2011-07-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-06-02" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>F6BF65</accession>
    <name>F6BF65_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="7">TM2 domain containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Metig_1400</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96935.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799531.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96935">
        <property type="protein sequence ID" value="AEF96935"/>
        <property type="gene ID" value="Metig_1400"/>
    </dbReference>
    <dbReference type="GeneID" id="10644273"/>
    <dbReference type="KEGG" id="mig:Metig_1400"/>
    <dbReference type="HOGENOM" id="CLU_2285050_0_0_2"/>
    <dbReference type="OMA" id="RIVYYEQ"/>
    <dbReference type="OrthoDB" id="113743at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007829">
        <property type="entry name" value="TM2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05154">
        <property type="entry name" value="TM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="36"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="61"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="domain" description="TM2" evidence="6">
        <location>
            <begin position="34"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF05154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEF96935.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="101" mass="11622" checksum="54E10BD4E9A561D4" modified="2011-07-27" version="1">MGGNRVDETEFMQIKEFVKDMDKTQRIVYYEQKKKSVGIAVLLSALIPGAGQMYLGKVGKGIIILLTFWFFLIPYIYGIYVAYKSAKDYNARLYSVIFSEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>F6BA73</accession>
    <name>F6BA73_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Isopropylmalate/isohomocitrate dehydrogenase</fullName>
            <ecNumber evidence="5">1.1.1.83</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Metig_0206</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the isocitrate and isopropylmalate dehydrogenases family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.83" evidence="5"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95763.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798372.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0206"/>
    <dbReference type="EnsemblBacteria" id="AEF95763">
        <property type="protein sequence ID" value="AEF95763"/>
        <property type="gene ID" value="Metig_0206"/>
    </dbReference>
    <dbReference type="GeneID" id="10643041"/>
    <dbReference type="KEGG" id="mig:Metig_0206"/>
    <dbReference type="HOGENOM" id="CLU_031953_0_1_2"/>
    <dbReference type="OMA" id="TCAHKAN"/>
    <dbReference type="OrthoDB" id="33452at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003862">
        <property type="term" value="F:3-isopropylmalate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046553">
        <property type="term" value="F:D-malate dehydrogenase (decarboxylating) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009098">
        <property type="term" value="P:leucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019818">
        <property type="entry name" value="IsoCit/isopropylmalate_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024084">
        <property type="entry name" value="IsoPropMal-DH-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011828">
        <property type="entry name" value="LEU3_arc"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00180">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01329">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02088">
        <property type="entry name" value="LEU3_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00470">
        <property type="entry name" value="IDH_IMDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Iso_dh" evidence="4">
        <location>
            <begin position="4"/>
            <end position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM01329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEF95763.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="334" mass="36381" checksum="2559BCC12692E84A" modified="2011-07-27" version="1">MAHKICVIEGDGIGKEVIPAAIKVLEATGVDFEFIHAEAGDEVFEKTGVALPEETIEKALECDAVFFGAAGETAADVIVKLRQILKTYANVRPVKAYKGIKCLRDDIDYVIVRENTEGLYKGLEAEIDEGVTTATRVITRKACERIFKFAFELARDRKKQGKEGKVTCAHKANVLKITDGLFKKVFYEVAEQYDDIKAEDYYIDAMNMYIITKPQTFDVVVTSNLFGDILSDGAAGTVGGLGLAPSANIGDEHGLFEPVHGSAPDIAGKGIANPTATILSGVLMLRYLKEFEAADKIERALEEVLAEGLTTPDLGGNLTTFQMAEEVAKRVEKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>F6BA75</accession>
    <name>F6BA75_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="3">CRISPR locus-related DNA-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_0208</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95765.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798374.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0208"/>
    <dbReference type="EnsemblBacteria" id="AEF95765">
        <property type="protein sequence ID" value="AEF95765"/>
        <property type="gene ID" value="Metig_0208"/>
    </dbReference>
    <dbReference type="GeneID" id="10643043"/>
    <dbReference type="KEGG" id="mig:Metig_0208"/>
    <dbReference type="HOGENOM" id="CLU_1352125_0_0_2"/>
    <dbReference type="OMA" id="GEHINHI"/>
    <dbReference type="OrthoDB" id="83805at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010163">
        <property type="entry name" value="Csa3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046260">
        <property type="entry name" value="DUF6293"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002831">
        <property type="entry name" value="Tscrpt_reg_TrmB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19810">
        <property type="entry name" value="DUF6293"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01978">
        <property type="entry name" value="TrmB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01884">
        <property type="entry name" value="cas_HTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF6293" evidence="2">
        <location>
            <begin position="3"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="domain" description="TrmB" evidence="1">
        <location>
            <begin position="139"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF19810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF95765.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="200" mass="23034" checksum="2C72F9B744E15C12" modified="2011-07-27" version="1">MTTHIINIGFHVEHIYKPIAEYGAKKVILVYSKDKEEYTKEEDLKKVDDALKKAKELCNMLGIECEEAKINGVEFEKNVEILRNIIKKEGKVIVNITGGRKITSLALLYASLYEFQKVHKIVYVHKKRIIELPKLAHPYNLTKFERKILVSLNEGDKTITDLAKEFNVSLPAIVKYVNSLENKGLIKTEKIGRRRIVKLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2020-06-17" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAA3</accession>
    <name>F6BAA3_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_0236</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95793.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF95793">
        <property type="protein sequence ID" value="AEF95793"/>
        <property type="gene ID" value="Metig_0236"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0236"/>
    <dbReference type="HOGENOM" id="CLU_3227807_0_0_2"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF95793.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="43" mass="5104" checksum="A83B785C7A442321" modified="2011-07-27" version="1">MDKVSFDKLVDIIFRELIPKLVYNRGVSKFAEERSKFEGWLKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAA4</accession>
    <name>F6BAA4_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glycosyl transferase family 9</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0237</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95794.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798403.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0237"/>
    <dbReference type="EnsemblBacteria" id="AEF95794">
        <property type="protein sequence ID" value="AEF95794"/>
        <property type="gene ID" value="Metig_0237"/>
    </dbReference>
    <dbReference type="GeneID" id="10643072"/>
    <dbReference type="KEGG" id="mig:Metig_0237"/>
    <dbReference type="HOGENOM" id="CLU_709057_0_0_2"/>
    <dbReference type="OMA" id="HMAVTQG"/>
    <dbReference type="OrthoDB" id="37526at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03789">
        <property type="entry name" value="GT9_LPS_heptosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002201">
        <property type="entry name" value="Glyco_trans_9"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01075">
        <property type="entry name" value="Glyco_transf_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF95794.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="389" mass="46403" checksum="2ACC62A3A5FCE2F0" modified="2011-07-27" version="1">MDNELKKLKNYFKYFVVKSVEIPFYIKYSLNTKKEIDYQKSFKKIALICTKPMGLGDLIMDTPFIKELRKNFPDSEIHLITDKDIFDKVREIDKIIIVKGNTLKLIKEFYKLKKEKYDLGIVMNRGINQTFYLEVLNPKYKLGYLAGWKILSNFKLKKEGLRFTKNEHYWDMALKILHSLGIEINKEELVEVDFNKDVKQNVEEIYKKLKIDENKKTIIINPFVRWRTRMWDADNYIKLIKEIHKDFNIILYGGKDSLKTVNYIVNKLKNEGIYIENVAGKLGLKEAIYFLKFADLFITSDSGPMHFAFLMKVPTLALFGPVNPLHRLPKNFKKYGIYDYLWYNDFKPLKKFYNYEYEYMDKELEGLKAIPVEAVKSKIYKFFENGRFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-06-02" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAE0</accession>
    <name>F6BAE0_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PK_C domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0274</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95830.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798439.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF95830">
        <property type="protein sequence ID" value="AEF95830"/>
        <property type="gene ID" value="Metig_0274"/>
    </dbReference>
    <dbReference type="GeneID" id="10643109"/>
    <dbReference type="KEGG" id="mig:Metig_0274"/>
    <dbReference type="HOGENOM" id="CLU_095207_1_0_2"/>
    <dbReference type="OMA" id="VTYHQGF"/>
    <dbReference type="OrthoDB" id="104210at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1380.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015074">
        <property type="entry name" value="DUF1867"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015795">
        <property type="entry name" value="Pyrv_Knase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036918">
        <property type="entry name" value="Pyrv_Knase_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02887">
        <property type="entry name" value="PK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016138">
        <property type="entry name" value="UCP016138"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52935">
        <property type="entry name" value="SSF52935"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PK_C" evidence="1">
        <location>
            <begin position="14"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF95830.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="195" mass="21282" checksum="4D6EEA6A7D3AAA93" modified="2011-07-27" version="1">MTVYFDYAGSQNTEQTLKLAVERAKKGDIKSIVVASSTGETAKKLLELLKKEGLNLNVVVVTYHQGFYGDDIKSMDDKTREELEKMGAKVFMGTHALSGVERGISRKLGGYGPVEVIAETLRTFGHGVKVCYEIAVMAADAGLVSVKEDIIAIGGRSRGADTAMVIRPANMNTFFNIEAKEIICMPRNKKKMGEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAR3</accession>
    <name>F6BAR3_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Formylmethanofuran dehydrogenase</fullName>
            <ecNumber evidence="3">1.2.7.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Metig_0321</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>H2O + N-formylmethanofuran + 2 oxidized [2Fe-2S]-[ferredoxin] = CO2 + H(+) + methanofuran + 2 reduced [2Fe-2S]-[ferredoxin]</text>
            <dbReference type="Rhea" id="RHEA:19841"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:57727"/>
            <dbReference type="ChEBI" id="CHEBI:58151"/>
            <dbReference type="EC" id="1.2.7.12"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">One-carbon metabolism; methanogenesis from CO(2); 5,10-methenyl-5,6,7,8-tetrahydromethanopterin from CO(2): step 1/3.</text>
    </comment>
    <dbReference type="EC" id="1.2.7.12" evidence="3"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95877.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798486.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0321"/>
    <dbReference type="EnsemblBacteria" id="AEF95877">
        <property type="protein sequence ID" value="AEF95877"/>
        <property type="gene ID" value="Metig_0321"/>
    </dbReference>
    <dbReference type="GeneID" id="10643157"/>
    <dbReference type="KEGG" id="mig:Metig_0321"/>
    <dbReference type="HOGENOM" id="CLU_661592_0_0_2"/>
    <dbReference type="OMA" id="NMHPEDY"/>
    <dbReference type="OrthoDB" id="29442at2157"/>
    <dbReference type="UniPathway" id="UPA00640">
        <property type="reaction ID" value="UER00692"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018493">
        <property type="term" value="F:formylmethanofuran dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030151">
        <property type="term" value="F:molybdenum ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043546">
        <property type="term" value="F:molybdopterin cofactor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019386">
        <property type="term" value="P:methanogenesis, from carbon dioxide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00980">
        <property type="entry name" value="FwdC/FmdC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009010">
        <property type="entry name" value="Asp_de-COase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012048">
        <property type="entry name" value="Formylmethanofuran_DH_csu/dsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017550">
        <property type="entry name" value="Formylmethanofuran_DH_suC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036485">
        <property type="entry name" value="Glu_synth_asu_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006657">
        <property type="entry name" value="MoPterin_dinucl-bd_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01568">
        <property type="entry name" value="Molydop_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036633">
        <property type="entry name" value="FmdC_D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50692">
        <property type="entry name" value="SSF50692"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69336">
        <property type="entry name" value="SSF69336"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03122">
        <property type="entry name" value="one_C_dehyd_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Molydop_binding" evidence="4">
        <location>
            <begin position="289"/>
            <end position="387"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004830"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEF95877.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="411" mass="44964" checksum="09DED456194958A7" modified="2011-07-27" version="1">MQVILTPKETPDIPIEADVITPDNFANKSKEEIESLMVWQGPNTYPLSEFFDVEVSSDDSEDVTIIIEGDVKRVKYIGYKMTSGKIIINGNVGIQLGSEMKGGEIVVNGNAGNWVGREMKGGLIKINGNAGDYVGSAYRGSWYGMKGGKIIVEGNAGNNVGGGLSGGEIIIKGNVGQFCGIRQSGGFIYIGGNAERAVGVEMSKGDIVVCGRIRFFAPGFEFANEEVDININGMQINGEFLKFVGDYAIKRNPKGKLYALKEKNLGLIEPELYECYESHRYDGGIKALLNTGSTIVQGEIIKGGKKFTEKYVKECAVCYIHPKDYAYLGKPKYVKVFSEDRKSEICLKAIPDDSLQEGTVFIPRSIWANVVISSYTEAMGSPLYKGCFVFVEPVKEAKILSAEEIMKRIYG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAS6</accession>
    <name>F6BAS6_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Coenzyme F420-reducing hydrogenase delta subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_0334</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95890.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798499.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0334"/>
    <dbReference type="EnsemblBacteria" id="AEF95890">
        <property type="protein sequence ID" value="AEF95890"/>
        <property type="gene ID" value="Metig_0334"/>
    </dbReference>
    <dbReference type="GeneID" id="10643171"/>
    <dbReference type="KEGG" id="mig:Metig_0334"/>
    <dbReference type="HOGENOM" id="CLU_099037_0_2_2"/>
    <dbReference type="OMA" id="GCGNLIF"/>
    <dbReference type="OrthoDB" id="122958at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008047">
        <property type="term" value="F:enzyme activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06064">
        <property type="entry name" value="H2MP_F420-Reduc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004411">
        <property type="entry name" value="Pept_A31_F420-red_hyd_d"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023430">
        <property type="entry name" value="Pept_HybD-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000671">
        <property type="entry name" value="Peptidase_A31"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30302">
        <property type="entry name" value="PTHR30302"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01750">
        <property type="entry name" value="HycI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00446">
        <property type="entry name" value="HYDRGNUPTAKE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53163">
        <property type="entry name" value="SSF53163"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00130">
        <property type="entry name" value="frhD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00072">
        <property type="entry name" value="hydrog_prot"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF95890.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="162" mass="17910" checksum="E049604177D76BCA" modified="2011-07-27" version="1">MLPKSLQKEILVFGCGNLLFADDGFGYEVISRLEKMDLPENVEIIDAGTGAPYYLMSIMDEEAKVKKIIIVDVIDFGLEPGTLKKVDVDELPKINKYTFDAHGTPLAPYLIDASKKGIEVVIIGCQAKEISMPEIKIGLSEEVKNAVDKAVEMVLEEIYKSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-09-29" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAT2</accession>
    <name>F6BAT2_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="3">AAA ATPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_0340</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the archaeal ATPase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95896.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF95896">
        <property type="protein sequence ID" value="AEF95896"/>
        <property type="gene ID" value="Metig_0340"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0340"/>
    <dbReference type="HOGENOM" id="CLU_068608_0_0_2"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011579">
        <property type="entry name" value="ATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01637">
        <property type="entry name" value="ATPase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA" evidence="2">
        <location>
            <begin position="53"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF95896.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="397" mass="46682" checksum="C5475815D478D20D" modified="2011-07-27" version="1">MKVVIFTLLILIPFVTDYNFNMLSTLYTNGEIMKFFNREKEIKEISVIIEGEPNLIYFIYGPINSGKTTLINHIINNELKKSNKKYAVFYVNFREYDISSMNNFVEALFEVDESSKSNKMREYIEYFTKGLNDIINISYGIKISEPLLDRLFGEKEKGRLVFKFIRDLFISLNNKGIQPVFILDELQMIKDIVMNGERPLLKSLFQFLVSLTKERHLAHCLCLTSDSLFIEYVYNTGELKDRSDYILVDDFDKEMALRFMDFLAEELLNKPLSEEQKELIYSTVGGKPILIHKVIDKMRYKELNTILDEMIKIEVSKLKKLLIRIKNNKFKGIDYNKVVNALSLFKKEYLIDEYTIDEDTKEFLIKENILFLNPQEGTLKPQSFLVWNAIKKVVYSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F6BAX7</accession>
    <name>F6BAX7_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Polysaccharide biosynthesis protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Metig_1530</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97064.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799658.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1530"/>
    <dbReference type="EnsemblBacteria" id="AEF97064">
        <property type="protein sequence ID" value="AEF97064"/>
        <property type="gene ID" value="Metig_1530"/>
    </dbReference>
    <dbReference type="GeneID" id="10644403"/>
    <dbReference type="KEGG" id="mig:Metig_1530"/>
    <dbReference type="HOGENOM" id="CLU_022017_6_2_2"/>
    <dbReference type="OMA" id="RTQYLIP"/>
    <dbReference type="OrthoDB" id="41033at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029303">
        <property type="entry name" value="Polysacc_synt_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002797">
        <property type="entry name" value="Polysacc_synth"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01943">
        <property type="entry name" value="Polysacc_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14667">
        <property type="entry name" value="Polysacc_synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="43"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="83"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="112"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="144"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="174"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="215"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="251"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="291"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="322"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="357"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="382"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="412"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="439"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="domain" description="Polysacc_synt_C" evidence="7">
        <location>
            <begin position="328"/>
            <end position="460"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF14667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEF97064.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="483" mass="54673" checksum="376D1B44F224A5CC" modified="2011-07-27" version="1">MGVAKRIAKNTGILFVSNIISKFFGFVYTVYMARYLGAEGFGILSFALAFTGMFGIIADFGLQPLTVREVARNPELTGKYLGNIAVIKSILGIITFILIVLTINLMNYPAETVYVVYLVAFYVLIGSFNNMFYSIYQAHEKMEYVGIGNILNSSLMLLGVFTAMYLGFNVEGFAYIYLISGIGVLLYNICVTLWKFVKPKIEIDLKFWKELLKEAWPFALSGFFITVYYWIDSVMLSYMVGNLVVGWYNAAYRIILVFLFIPIIVNAAIYPIMSRYYITSNKSLKIICEKYFKIMLTIGLPLGILITVFAEEVVLSIFGQDYHGTIIALKILIWGLVSIFANAPFVKLFESINRQSLVTKVTGFAMVMNIILNILLIPKFSYIGASITTLVTEFTIAVFVIILGIKLKYVEFANILNILIRLTVCGIFMFVYLKISVLFLGTIISAISSLIVYSLLLYIMVLDKDDKKILKNLFNKKKRWILC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>F6BB17</accession>
    <name>F6BB17_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Methanogen homoaconitase small subunit</fullName>
            <shortName evidence="2">HACN</shortName>
            <ecNumber evidence="2">4.2.1.114</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">Homoaconitate hydratase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2" type="primary">hacB</name>
        <name evidence="4" type="ordered locus">Metig_1570</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Hydro-lyase with broad substrate specificity for cis-unsaturated tricarboxylic acids. Catalyzes both the reversible dehydration of (R)-homocitrate ((R)-2-hydroxybutane-1,2,4-tricarboxylate) to produce cis-homoaconitate ((Z)-but-1-ene-1,2,4-tricarboxylate), and its hydration to homoisocitrate ((1R,2S)-1-hydroxybutane-1,2,4-tricarboxylate). Is also able to hydrate the analogous longer chain substrates cis-homo(2)-aconitate, cis-homo(3)-aconitate. All these reactions are part of the biosynthesis pathway of coenzyme B.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>(2R)-homocitrate = (2R,3S)-homoisocitrate</text>
            <dbReference type="Rhea" id="RHEA:32303"/>
            <dbReference type="ChEBI" id="CHEBI:15404"/>
            <dbReference type="ChEBI" id="CHEBI:58884"/>
            <dbReference type="EC" id="4.2.1.114"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Organic acid metabolism; 2-oxosuberate biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Heterotetramer of 2 HacA and 2 HacB proteins.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the LeuD family. LeuD type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.114" evidence="2"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97104.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799698.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1570"/>
    <dbReference type="EnsemblBacteria" id="AEF97104">
        <property type="protein sequence ID" value="AEF97104"/>
        <property type="gene ID" value="Metig_1570"/>
    </dbReference>
    <dbReference type="GeneID" id="10644443"/>
    <dbReference type="KEGG" id="mig:Metig_1570"/>
    <dbReference type="HOGENOM" id="CLU_081378_1_1_2"/>
    <dbReference type="OMA" id="APFMVGE"/>
    <dbReference type="OrthoDB" id="86621at2157"/>
    <dbReference type="UniPathway" id="UPA00919"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004409">
        <property type="term" value="F:homoaconitate hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019298">
        <property type="term" value="P:coenzyme B biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01577">
        <property type="entry name" value="IPMI_Swivel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.19.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01032">
        <property type="entry name" value="LeuD_type2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015928">
        <property type="entry name" value="Aconitase/3IPM_dehydase_swvl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000573">
        <property type="entry name" value="AconitaseA/IPMdHydase_ssu_swvl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033940">
        <property type="entry name" value="IPMI_Swivel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011827">
        <property type="entry name" value="LeuD_type2/HacB/DmdB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00694">
        <property type="entry name" value="Aconitase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02087">
        <property type="entry name" value="LEUD_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 2 4" id="KW-0456">Lyase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aconitase_C" evidence="3">
        <location>
            <begin position="41"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="YLRT" evidence="2">
        <location>
            <begin position="24"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="site" description="Critical for substrate specificity" evidence="2">
        <location>
            <position position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEF97104.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="169" mass="18577" checksum="7B850561A995762E" modified="2011-07-27" version="1">MKIRGRAHVFGDDVDTDAIIPGPYLRTTDVYELASHCMAGIDEDFPKKVKEGDVIVAGENFGCGSSREQAPIAIKYCGIKAVIAESFARIFYRNAINIGLIPIVCKGITKHVKDGDVIEVDLENEKIIINDKITLNCETPKGIEREILEAGGLTNYIKKQQNLKKVASN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-02-10" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>F6BB61</accession>
    <name>F6BB61_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Integrase catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0390</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95946.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF95946">
        <property type="protein sequence ID" value="AEF95946"/>
        <property type="gene ID" value="Metig_0390"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0390"/>
    <dbReference type="HOGENOM" id="CLU_2044423_0_0_2"/>
    <dbReference type="OMA" id="NLECICL"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00665">
        <property type="entry name" value="rve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="1">
        <location>
            <begin position="135"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF95946.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="298" mass="35431" checksum="2C1F307B473359A9" modified="2011-07-27" version="1">MKGAKLNDKKIRKIIRWKEKGFETSRIAKKVGVTPRRVQQIWKEYKETGEIPKIKRRGRKPKKISKEEIDLVLKAVEEYKGTSAVHLEKYIESKHNIHIPHNRIYTISYIYYTEGIRPHPQEKEEKKKKKPKRFSASKPNEMWQMDFKIVNVDGVRYNLLLIIDDHSRFILHAGIYEESTTDVVISALEECFEKHGTPKKILTDNGTQFVPARGGISRFQKFLMEREILHIKTSIRHPQTIGKVERINREVEYRLDKFNNLKEFVEWHNQIKPHRSLSFKTPYEVYFENTKCNKEVIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>F6BB77</accession>
    <name>F6BB77_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ribonuclease P protein component 4</fullName>
            <shortName evidence="2">RNase P component 4</shortName>
            <ecNumber evidence="2">3.1.26.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">Rpp21</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2" type="primary">rnp4</name>
        <name evidence="3" type="ordered locus">Metig_0406</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Part of ribonuclease P, a protein complex that generates mature tRNA molecules by cleaving their 5'-ends.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>Endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor.</text>
            <dbReference type="EC" id="3.1.26.5"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="2">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Consists of a catalytic RNA component and at least 4-5 protein subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eukaryotic/archaeal RNase P protein component 4 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.5" evidence="2"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95962.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798571.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0406"/>
    <dbReference type="EnsemblBacteria" id="AEF95962">
        <property type="protein sequence ID" value="AEF95962"/>
        <property type="gene ID" value="Metig_0406"/>
    </dbReference>
    <dbReference type="GeneID" id="10643243"/>
    <dbReference type="KEGG" id="mig:Metig_0406"/>
    <dbReference type="HOGENOM" id="CLU_079140_3_1_2"/>
    <dbReference type="OMA" id="HVVITCL"/>
    <dbReference type="OrthoDB" id="128960at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030677">
        <property type="term" value="C:ribonuclease P complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004526">
        <property type="term" value="F:ribonuclease P activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001682">
        <property type="term" value="P:tRNA 5'-leader removal"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00757">
        <property type="entry name" value="RNase_P_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016432">
        <property type="entry name" value="RNP4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007175">
        <property type="entry name" value="Rpr2/Snm1/Rpp21"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04032">
        <property type="entry name" value="Rpr2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004878">
        <property type="entry name" value="RNase_P_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1 2" id="KW-0540">Nuclease</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <feature type="binding site" evidence="2">
        <location>
            <position position="64"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="67"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="93"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="96"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF95962.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="124" mass="15262" checksum="ECE812B7AF1BEC16" modified="2011-07-27" version="1">MKYRRMLKRAKKIALERIEILMNLAEEEAKKGNWKRVKRYVSLARRIAMKVRVRFPKKWKRRICRKCNTLLVYGKNARVRVKSRRYPHVVITCLECGRIYRIPMIREKKEKMQKKRISNNIFRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>F6BB98</accession>
    <name>F6BB98_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_0427</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95983.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798592.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0427"/>
    <dbReference type="EnsemblBacteria" id="AEF95983">
        <property type="protein sequence ID" value="AEF95983"/>
        <property type="gene ID" value="Metig_0427"/>
    </dbReference>
    <dbReference type="GeneID" id="10643264"/>
    <dbReference type="KEGG" id="mig:Metig_0427"/>
    <dbReference type="HOGENOM" id="CLU_2366171_0_0_2"/>
    <dbReference type="OMA" id="TNHPLYE"/>
    <dbReference type="OrthoDB" id="112562at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036825">
        <property type="entry name" value="MTH865-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024093">
        <property type="entry name" value="Uncharacterised_MTH865"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07747">
        <property type="entry name" value="MTH865"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69025">
        <property type="entry name" value="SSF69025"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF95983.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="92" mass="10402" checksum="BC4625060DF6DB57" modified="2011-07-27" version="1">MITTNHPLYEALKDIQDFKLRLVEFFKDKKVFPIKSKVELANALPCGLSLPCGEIEAGELVKLLNEDDFPIKDAEDLAVKLANKCLIEKENK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBA1</accession>
    <name>F6BBA1_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Rubredoxin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0430</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95986.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798595.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0430"/>
    <dbReference type="EnsemblBacteria" id="AEF95986">
        <property type="protein sequence ID" value="AEF95986"/>
        <property type="gene ID" value="Metig_0430"/>
    </dbReference>
    <dbReference type="GeneID" id="10643267"/>
    <dbReference type="KEGG" id="mig:Metig_0430"/>
    <dbReference type="HOGENOM" id="CLU_202417_0_0_2"/>
    <dbReference type="OMA" id="DVTCYTP"/>
    <dbReference type="OrthoDB" id="118471at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024934">
        <property type="entry name" value="Rubredoxin-like_dom"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50903">
        <property type="entry name" value="RUBREDOXIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rubredoxin-like" evidence="1">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF95986.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="59" mass="6793" checksum="E42BD26772877899" modified="2011-07-27" version="1">MTWWKCSNCGYLFEADTIPEKCPNCGEKCTFYDVTCYTPECGCQGYDPKLVARKDPRIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBG8</accession>
    <name>F6BBG8_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Nucleic acid binding OB-fold tRNA/helicase-type</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_1643</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97175.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1643"/>
    <dbReference type="EnsemblBacteria" id="AEF97175">
        <property type="protein sequence ID" value="AEF97175"/>
        <property type="gene ID" value="Metig_1643"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_1643"/>
    <dbReference type="HOGENOM" id="CLU_116581_0_0_2"/>
    <dbReference type="OMA" id="KRDKYRH"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035451">
        <property type="entry name" value="Ada-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004365">
        <property type="entry name" value="NA-bd_OB_tRNA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01336">
        <property type="entry name" value="tRNA_anti-codon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57884">
        <property type="entry name" value="SSF57884"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0347">Helicase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="domain" description="OB" evidence="2">
        <location>
            <begin position="63"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF97175.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="202" mass="23564" checksum="9135D41573D69F14" modified="2011-07-27" version="1">MNDKHVIAFCLIFAVLGLISLNFYTLEPKDVKICEIKDGDYVKITGYIQKLIVKRDEYRHILEVKSLTISDGTGHLNVVAFGKVREELTNYIKDYQPCSIKEEDKVQVIGKISTYNGRYQLILEDIDNFKLIEKLNFEKDIYLSPIPTHIYASKYGKTYHTSEDCPYGKKIKKDNRIYFYSEEDAEELGYKKCKWCSKNANK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBR4</accession>
    <name>F6BBR4_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">50S ribosomal protein L7Ae</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Ribosomal protein L8e</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">rpl7ae</name>
        <name evidence="10" type="ordered locus">Metig_1662</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Multifunctional RNA-binding protein that recognizes the K-turn motif in ribosomal RNA, the RNA component of RNase P, box H/ACA, box C/D and box C'/D' sRNAs.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Part of the 50S ribosomal subunit. Probably part of the RNase P complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the eukaryotic ribosomal protein eL8 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97194.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799785.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1662"/>
    <dbReference type="EnsemblBacteria" id="AEF97194">
        <property type="protein sequence ID" value="AEF97194"/>
        <property type="gene ID" value="Metig_1662"/>
    </dbReference>
    <dbReference type="GeneID" id="10644535"/>
    <dbReference type="KEGG" id="mig:Metig_1662"/>
    <dbReference type="HOGENOM" id="CLU_084513_4_0_2"/>
    <dbReference type="OMA" id="KPFYVRF"/>
    <dbReference type="OrthoDB" id="114541at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004526">
        <property type="term" value="F:ribonuclease P activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001682">
        <property type="term" value="P:tRNA 5'-leader removal"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00326">
        <property type="entry name" value="Ribosomal_L7Ae_Arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029064">
        <property type="entry name" value="L30e-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004038">
        <property type="entry name" value="Ribosomal_L7Ae/L30e/S12e/Gad45"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018492">
        <property type="entry name" value="Ribosomal_L7Ae/L8/Nhp2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022481">
        <property type="entry name" value="Ribosomal_L7Ae_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004037">
        <property type="entry name" value="Ribosomal_L7Ae_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000948">
        <property type="entry name" value="Ribosomal_L7Ae_prok"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01248">
        <property type="entry name" value="Ribosomal_L7Ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00881">
        <property type="entry name" value="L7ARS6FAMILY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00884">
        <property type="entry name" value="RIBOSOMALHS6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55315">
        <property type="entry name" value="SSF55315"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03677">
        <property type="entry name" value="eL8_ribo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01082">
        <property type="entry name" value="RIBOSOMAL_L7AE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 8" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="6 8 10" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="5 8" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4 8" id="KW-0699">rRNA-binding</keyword>
    <keyword evidence="3 8" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="Ribosomal_L7Ae" evidence="9">
        <location>
            <begin position="19"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AEF97194.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="117" mass="12704" checksum="D486519AF079C948" modified="2011-07-27" version="1">MAIYVKFKVPEEIQELTYKVVSNAEKIKKGANEVTKAVEKGIAKLVIIAEDVQPEEIVAHLPPLCEEKGIPYTYVASKQELGKAAGLEVAASSVAIINEGNADELKDLIEKVNALKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBS5</accession>
    <name>F6BBS5_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_1673</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97205.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799796.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF97205">
        <property type="protein sequence ID" value="AEF97205"/>
        <property type="gene ID" value="Metig_1673"/>
    </dbReference>
    <dbReference type="GeneID" id="10644546"/>
    <dbReference type="KEGG" id="mig:Metig_1673"/>
    <dbReference type="HOGENOM" id="CLU_117970_0_0_2"/>
    <dbReference type="OMA" id="GMTDQTI"/>
    <dbReference type="OrthoDB" id="77688at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="191"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="20"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF97205.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="211" mass="24233" checksum="10C09B2F58DADA00" modified="2011-07-27" version="1">MLTKLKNLFSKNKNPLNIPLNKLKESKIKLDIQVKKLEDEISKIDNQIAMLFEKAKSSKSKSEELTIATKIKTLSQRKKQLQNSHALLNKQMRFVDNLLVIKENEEILKSTPLWNTLNSMSPQELENHLINMKINSENIIGTLNNALGITDDMLSSASEEHDEDIADILNTIHAVKEGELDIDDAKEIVSDEKKQEKEDDFERELRKVLKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>F6BBT9</accession>
    <name>F6BBT9_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Lysylphosphatidylglycerol synthetase/UPF0104</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Metig_1687</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0104 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF97219.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799809.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1687"/>
    <dbReference type="EnsemblBacteria" id="AEF97219">
        <property type="protein sequence ID" value="AEF97219"/>
        <property type="gene ID" value="Metig_1687"/>
    </dbReference>
    <dbReference type="GeneID" id="10644560"/>
    <dbReference type="KEGG" id="mig:Metig_1687"/>
    <dbReference type="HOGENOM" id="CLU_048072_1_1_2"/>
    <dbReference type="OMA" id="FINNVTP"/>
    <dbReference type="OrthoDB" id="36454at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022791">
        <property type="entry name" value="L-PG_synthase/AglD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03706">
        <property type="entry name" value="LPG_synthase_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00374">
        <property type="entry name" value="TIGR00374"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="42"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="130"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="155"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="227"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="255"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="286"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="311"/>
            <end position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEF97219.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="345" mass="39757" checksum="D3848C08F63A6BA8" modified="2011-07-27" version="1">MANKSKTKILRNLLFFILGILIIFAIIFYIGIYEIIEVLMKTNPVCFTLAFFIYLFTLVALVIRWKYLIRIIGFNAKFKNLFLLILMGQFINNITPSMKGGSEPFRAYYLSKLENIPYHITFSTVVVERLLDSAVFLLLTSIVILYFLINGIMYAKIFILVWFLILCLTILSIYILMHKRLAFRIVLKITKLISKFSSKTFDEEKINKSIEEFQNSLLFFKGRKKELIVCGILSLLWWILDIFRVYIIFVSISYFAPFFVIAATYLISLLVGILPTLPGGLGTSDTVMIATYSVLNVPSSIAAVGTLLDRFISYWLATLMGAIALKVIRIRINMQNEHENFKQKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>F6BC35</accession>
    <name>F6BC35_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Succinate dehydrogenase and fumarate reductase iron-sulfur protein</fullName>
            <ecNumber evidence="3">1.3.99.1</ecNumber>
            <ecNumber evidence="3">1.8.98.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_0562</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.3.99.1" evidence="3"/>
    <dbReference type="EC" id="1.8.98.1" evidence="3"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96116.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0562"/>
    <dbReference type="EnsemblBacteria" id="AEF96116">
        <property type="protein sequence ID" value="AEF96116"/>
        <property type="gene ID" value="Metig_0562"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0562"/>
    <dbReference type="HOGENOM" id="CLU_023081_2_0_2"/>
    <dbReference type="OMA" id="VYQDACH"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051912">
        <property type="term" value="F:CoB--CoM heterodisulfide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00207">
        <property type="entry name" value="fer2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1060.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036010">
        <property type="entry name" value="2Fe-2S_ferredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001041">
        <property type="entry name" value="2Fe-2S_ferredoxin-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006058">
        <property type="entry name" value="2Fe2S_fd_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004017">
        <property type="entry name" value="Cys_rich_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009051">
        <property type="entry name" value="Helical_ferredxn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004489">
        <property type="entry name" value="Succ_DH/fum_Rdtase_Fe-S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025192">
        <property type="entry name" value="Succ_DH/fum_Rdtase_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02754">
        <property type="entry name" value="CCG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13085">
        <property type="entry name" value="Fer2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13183">
        <property type="entry name" value="Fer4_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54292">
        <property type="entry name" value="SSF54292"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00384">
        <property type="entry name" value="dhsB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00197">
        <property type="entry name" value="2FE2S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51085">
        <property type="entry name" value="2FE2S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="2Fe-2S ferredoxin-type" evidence="1">
        <location>
            <begin position="6"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="2">
        <location>
            <begin position="178"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF96116.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="493" mass="56572" checksum="550E3A3BF9BAE082" modified="2011-07-27" version="1">MEMVKIKVKRFNKDKDGFEEYEVPKGINVLNALEYINKNYHANIYFRSSCRAGQCGSCAMCINGEPKLACKTKVEDGMVIEPLKNFDVIRDLVVDRTPYYKKLANLRNYIERKEYPEDLENIKPDDINKVKKVRGCIDCLSCLSVCPVRKFTDYAGPTFMRQLARFAFDVRDEGDREKIAYFENLYNCTTCAKCVEVCPKEIEIVHHAIEKLRELCFKKGYYLKNHLAVRENVLKYNRSVEKDKTPLLEEVNEEYLVENEKMRVAFFTGCLIDYRLQNVGRDAIKVLNAHGISVIIPKNQVCCGSPFIRTGQTDIAEKLKKKNLEIFNNLDVDCVVTVCAGCGSTLKNDYKEKEFEVMDITEVLNKVGLIKYKPLDATVTYHDPCHLKRGQGVYDEPRKILKSIPNLKFIEMEVPDQCCGAGGGVRSGKPEVAYALGKRKAKMIFDTNVDYLVTVCPFCEYHIRDSLKKYKEENNIKKDIKVMNIVSLLSKVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCE4</accession>
    <name>F6BCE4_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Magnesium chelatase</fullName>
            <ecNumber evidence="1">6.6.1.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_0601</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EC" id="6.6.1.1" evidence="1"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96155.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0601"/>
    <dbReference type="EnsemblBacteria" id="AEF96155">
        <property type="protein sequence ID" value="AEF96155"/>
        <property type="gene ID" value="Metig_0601"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0601"/>
    <dbReference type="HOGENOM" id="CLU_002017_1_2_2"/>
    <dbReference type="OMA" id="YQFQGGL"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016851">
        <property type="term" value="F:magnesium chelatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009058">
        <property type="term" value="P:biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd10150">
        <property type="entry name" value="CobN_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003672">
        <property type="entry name" value="CobN/Mg_chltase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44119">
        <property type="entry name" value="PTHR44119"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02514">
        <property type="entry name" value="CobN-Mg_chel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0436">Ligase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF96155.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="1204" mass="138471" checksum="3B15B4F678839221" modified="2011-07-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2020-12-02" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCG1</accession>
    <name>F6BCG1_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ATP synthase H subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0622</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96172.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96172">
        <property type="protein sequence ID" value="AEF96172"/>
        <property type="gene ID" value="Metig_0622"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0622"/>
    <dbReference type="HOGENOM" id="CLU_177461_1_0_2"/>
    <dbReference type="OMA" id="TIERWKE"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014275">
        <property type="entry name" value="ATPase_A1A0-cplx_hsu"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02926">
        <property type="entry name" value="AhaH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="7"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96172.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="102" mass="11500" checksum="BC9C09B725930A35" modified="2011-07-27" version="1">MIAVDAIKQIKQVEEDAIKEIEDAKKRAEEIKLEAIEKGKELIKKAEEEAQAKVEELIKNAEEDAKKEAEEIISKAKADIKDIVSVAKVKVLSLKLEEIIEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCG2</accession>
    <name>F6BCG2_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">V-type ATP synthase subunit I</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">Metig_0623</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the V-ATPase 116 kDa subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96173.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0623"/>
    <dbReference type="EnsemblBacteria" id="AEF96173">
        <property type="protein sequence ID" value="AEF96173"/>
        <property type="gene ID" value="Metig_0623"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0623"/>
    <dbReference type="HOGENOM" id="CLU_025558_1_0_2"/>
    <dbReference type="OMA" id="MAVNIMA"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033179">
        <property type="term" value="C:proton-transporting V-type ATPase, V0 domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002490">
        <property type="entry name" value="V-ATPase_116kDa_su"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11629">
        <property type="entry name" value="PTHR11629"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01496">
        <property type="entry name" value="V_ATPase_I"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7 9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="381"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="417"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="488"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="523"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="547"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="586"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="616"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="243"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AEF96173.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="680" mass="75423" checksum="40E2FDC56E64942C" modified="2011-07-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCH0</accession>
    <name>F6BCH0_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF22 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0631</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96181.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798786.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96181">
        <property type="protein sequence ID" value="AEF96181"/>
        <property type="gene ID" value="Metig_0631"/>
    </dbReference>
    <dbReference type="GeneID" id="10643470"/>
    <dbReference type="KEGG" id="mig:Metig_0631"/>
    <dbReference type="HOGENOM" id="CLU_152996_1_0_2"/>
    <dbReference type="OMA" id="LLICPYG"/>
    <dbReference type="OrthoDB" id="106591at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002572">
        <property type="entry name" value="DUF22"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01629">
        <property type="entry name" value="DUF22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF22" evidence="1">
        <location>
            <begin position="16"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96181.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="125" mass="14124" checksum="2B162925BE684FE5" modified="2011-07-27" version="1">MFRILGRVGKIEHDIEETKKYHYDFKFKEGGKIVPIVADETVEFKQGDAKSIKIKEIKIPPKHLVLICSYARHRVGHVIAMGEEFPVPVEMERSADRAMFIAGIDGTVSKDDLIGMALLIPIETM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCI1</accession>
    <name>F6BCI1_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">7,8-didemethyl-8-hydroxy-5-deazariboflavin synthase</fullName>
            <ecNumber evidence="3 9">4.3.1.32</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">FO synthase subunit 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">cofG</name>
        <name evidence="11" type="ordered locus">Metig_0642</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the radical-mediated synthesis of 7,8-didemethyl-8-hydroxy-5-deazariboflavin (FO) from 5-amino-5-(4-hydroxybenzyl)-6-(D-ribitylimino)-5,6-dihydrouracil.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>5-amino-5-(4-hydroxybenzyl)-6-(D-ribitylimino)-5,6-dihydrouracil + S-adenosyl-L-methionine = 5'-deoxyadenosine + 7,8-didemethyl-8-hydroxy-5-deazariboflavin + H(+) + L-methionine + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:55204"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:59904"/>
            <dbReference type="ChEBI" id="CHEBI:85936"/>
            <dbReference type="EC" id="4.3.1.32"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="9">Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 9">Cofactor biosynthesis; coenzyme F0 biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">The FO synthase complex consists of two subunits, CofG and CofH.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the radical SAM superfamily. CofG family.</text>
    </comment>
    <dbReference type="EC" id="4.3.1.32" evidence="3 9"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96192.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798796.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0642"/>
    <dbReference type="EnsemblBacteria" id="AEF96192">
        <property type="protein sequence ID" value="AEF96192"/>
        <property type="gene ID" value="Metig_0642"/>
    </dbReference>
    <dbReference type="GeneID" id="10643482"/>
    <dbReference type="KEGG" id="mig:Metig_0642"/>
    <dbReference type="HOGENOM" id="CLU_054174_0_0_2"/>
    <dbReference type="OMA" id="TTACRYT"/>
    <dbReference type="OrthoDB" id="52096at2157"/>
    <dbReference type="UniPathway" id="UPA00072"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016841">
        <property type="term" value="F:ammonia-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016765">
        <property type="term" value="F:transferase activity, transferring alkyl or aryl (other than methyl) groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01611">
        <property type="entry name" value="FO_synth_sub1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019939">
        <property type="entry name" value="CofG_family"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaB/NifB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034405">
        <property type="entry name" value="F420"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43076">
        <property type="entry name" value="PTHR43076"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00294">
        <property type="entry name" value="7_8-didemethyl-8-hydroxy-5-dea"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03550">
        <property type="entry name" value="F420_cofG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="6 9" id="KW-0408">Iron</keyword>
    <keyword evidence="7 9" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="8 9" id="KW-0456">Lyase</keyword>
    <keyword evidence="5 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="10">
        <location>
            <begin position="37"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="51"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="55"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="58"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004712"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AEF96192.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="355" mass="41316" checksum="0D4C7FD7B2AC312E" modified="2011-07-27" version="1">MISREEAIAFLNSNSPKDILEKINLINELEKDREKYITYSKNVFIPLCNWCRNKCGYCIFRSNNPKLMKKEEVREILLKGDELGCREALFTFGERVDEHPKIKEELKKMGYSGILEYLYDLEDWCLSNTNLLPHTNCGILTYEELKMLREVNASMGLMLENVSERLMKTIAHKDSPGKEPKLRIEMIENAGKLKIPFTTGILIGIGETNEEIVDSILKIKEIHEKYGHIQEVIIQNFRTKKGIPMENFEEPSPIKMLKVIILAKLMLDDISIQVPPNLNKETGQLFLLAGIDDWGGVSPLTKDYVNPEAPWPEIEELRRFTEELGFKLKMRLPVYDKYIKKEWLSERIYEKIVKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCJ6</accession>
    <name>F6BCJ6_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="8">MscS Mechanosensitive ion channel</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Metig_0657</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MscS (TC 1.A.23) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96207.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798810.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0657"/>
    <dbReference type="EnsemblBacteria" id="AEF96207">
        <property type="protein sequence ID" value="AEF96207"/>
        <property type="gene ID" value="Metig_0657"/>
    </dbReference>
    <dbReference type="GeneID" id="10643497"/>
    <dbReference type="KEGG" id="mig:Metig_0657"/>
    <dbReference type="HOGENOM" id="CLU_066007_1_0_2"/>
    <dbReference type="OMA" id="IMEIREW"/>
    <dbReference type="OrthoDB" id="57211at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006685">
        <property type="entry name" value="MscS_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011066">
        <property type="entry name" value="MscS_channel_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011014">
        <property type="entry name" value="MscS_channel_TM-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023408">
        <property type="entry name" value="MscS_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00924">
        <property type="entry name" value="MS_channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="SSF50182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82689">
        <property type="entry name" value="SSF82689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82861">
        <property type="entry name" value="SSF82861"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="7"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="45"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="78"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEF96207.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="332" mass="38276" checksum="27C6F346231F78C3" modified="2011-07-27" version="1">MRKIQVFRIKFITKVIILLGILYYLGCKLNFYQYLLKLGDYINQIIILTALILGTLIFLDITLELLRKYFEKVDIREYPIVASVVKYATWFGVILIAISVVYKDVGSLVMSLGLVGAALTLALQKPIMNFVGWLTIVFTHPFKINDRIYIKGIGGGDVYKIGTMYVSLREVDGEPTGRSLNIPNSYILTNPVINFSKGSPYVWDSVKVTITYESNWRKAEELILEACDEIVGEEMRRLAEIWKNKPRIFAKSKIYDKPVIRMRFLDSGIEIKVRYLVNVFEWAAIKTKIVKNILSKLEEVNDVEIAYPHMEVIHRQKSDIVDNPFFKGKKEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-09-29" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCK2</accession>
    <name>F6BCK2_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="3">AAA ATPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_0663</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the archaeal ATPase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96213.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798816.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96213">
        <property type="protein sequence ID" value="AEF96213"/>
        <property type="gene ID" value="Metig_0663"/>
    </dbReference>
    <dbReference type="GeneID" id="10643503"/>
    <dbReference type="KEGG" id="mig:Metig_0663"/>
    <dbReference type="HOGENOM" id="CLU_068608_0_0_2"/>
    <dbReference type="OrthoDB" id="34326at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011579">
        <property type="entry name" value="ATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01637">
        <property type="entry name" value="ATPase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA" evidence="2">
        <location>
            <begin position="23"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF96213.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="339" mass="40581" checksum="4417EB0F39E0E2C9" modified="2011-07-27" version="1">MQIKFYDREKELNYLKTYCQLIPNSILFVYGPKSSGKSTVMKRVIKDLEDREDLVFFYYNLRKYATPTKEEFLNVFFEKGDKKYLRNTLELNFKIFKFGVDEDYDFKNVSLNDVFRKINEDINAVVEDGKKPILIIDELQKLKSIYFNGSKSLLNELFNLFVSLTKMEHLCHVICLTSDSLFINEIYNNSSLAKTSKYYLIDWLEKETIEKILKEEKFNEEEINYAIKYLSLPYEISDLIENKKLGISVEKTIKRWINIERDRIKYTIDTSNFDENDIINILKKFENKIKIDYIDVKREEFEIFKYLIENEILFYDVINGIIKPQSLTTHYAIKELIRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-06-02" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCT6</accession>
    <name>F6BCT6_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_0750</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96297.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798900.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96297">
        <property type="protein sequence ID" value="AEF96297"/>
        <property type="gene ID" value="Metig_0750"/>
    </dbReference>
    <dbReference type="GeneID" id="10643589"/>
    <dbReference type="KEGG" id="mig:Metig_0750"/>
    <dbReference type="HOGENOM" id="CLU_150439_0_0_2"/>
    <dbReference type="OMA" id="IIIELPQ"/>
    <dbReference type="OrthoDB" id="132013at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF96297.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="122" mass="14541" checksum="F6ACA587330DD8EB" modified="2011-07-27" version="1">MPTKIIIELPQTLTKKEAIEILKREALKKKFKKTKLFEKYSKNKDIAFKIAEYVDKYLKRYYKDLKYEILLDYDLDDEVNIIRVFVKGIDLDNQLQIEEKLDKIINSKFENASLHNIVIVEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCV4</accession>
    <name>F6BCV4_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0769</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96315.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96315">
        <property type="protein sequence ID" value="AEF96315"/>
        <property type="gene ID" value="Metig_0769"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0769"/>
    <dbReference type="HOGENOM" id="CLU_1631690_0_0_2"/>
    <dbReference type="OMA" id="MYNEICI"/>
    <dbReference type="OrthoDB" id="80254at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="43"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96315.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="153" mass="18388" checksum="3F28A4EC17406913" modified="2011-07-27" version="1">MEEMDLKKIAELIILKDKDFEEKDKLKELLVKYVKIRDEIGILESILEDFEELDIKLKNLAKDIEITEKLLDKLNKNINISNYNEIKKLFKKFKSIEISLDESSRWDIYHKIETLKKDLEDVERQLEFAILNYAIAKTGNDNYLELMRYLEER</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCV6</accession>
    <name>F6BCV6_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Tryptophan--tRNA ligase</fullName>
            <ecNumber evidence="7">6.1.1.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Tryptophanyl-tRNA synthetase</fullName>
            <shortName evidence="7">TrpRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">trpS</name>
        <name evidence="9" type="ordered locus">Metig_0771</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the attachment of tryptophan to tRNA(Trp).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>ATP + L-tryptophan + tRNA(Trp) = AMP + diphosphate + H(+) + L-tryptophanyl-tRNA(Trp)</text>
            <dbReference type="Rhea" id="RHEA:24080"/>
            <dbReference type="Rhea" id="RHEA-COMP:9671"/>
            <dbReference type="Rhea" id="RHEA-COMP:9705"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57912"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78535"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.2"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 7 8">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.2" evidence="7"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96317.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798920.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0771"/>
    <dbReference type="EnsemblBacteria" id="AEF96317">
        <property type="protein sequence ID" value="AEF96317"/>
        <property type="gene ID" value="Metig_0771"/>
    </dbReference>
    <dbReference type="GeneID" id="10643611"/>
    <dbReference type="KEGG" id="mig:Metig_0771"/>
    <dbReference type="HOGENOM" id="CLU_032621_3_0_2"/>
    <dbReference type="OMA" id="SIYHRFM"/>
    <dbReference type="OrthoDB" id="33997at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004830">
        <property type="term" value="F:tryptophan-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006436">
        <property type="term" value="P:tryptophanyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00806">
        <property type="entry name" value="TrpRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00140_A">
        <property type="entry name" value="Trp_tRNA_synth_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002305">
        <property type="entry name" value="aa-tRNA-synth_Ic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002306">
        <property type="entry name" value="Trp-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020653">
        <property type="entry name" value="Tryptophan-tRNA-ligase_arc"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00579">
        <property type="entry name" value="tRNA-synt_1b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01039">
        <property type="entry name" value="TRNASYNTHTRP"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00233">
        <property type="entry name" value="trpS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7 8" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="4 7 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 7 8" id="KW-0436">Ligase</keyword>
    <keyword evidence="3 7 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 7 8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="short sequence motif" description="'HIGH' region" evidence="7">
        <location>
            <begin position="71"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'KMSKS' region" evidence="7">
        <location>
            <begin position="252"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AEF96317.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="367" mass="42265" checksum="F80B83AD5697513B" modified="2011-07-27" version="1">MITPWDVSDNIDYKKTMENFGIKPINEIIKNIENPHYLMRRGIIFGHRDFERIIDAMKNKKKFAVVSGMMPSGRMHFGHKMIVDQLLYYQDYNAEIHIPIADMEAYAARGMDFETTKKLAVEEYITNYIALGLDPEKINVYLQSKYNKVKDLAYILSKRTNLSEMRAIYGFGGETNIGHLFAPLIQVADILHPQLEDDLKTSRGIPVVVPVGIDQDPHIRLTRDIANRAKEYKFIPPSSTYHRFMTGLLGGKMSSSKPDTAIFLTDDEKSVKKKIMSCKTGGRSTLEEHKKLGGIPEECVVYELYAYHLIKDDKELLEIYEKCKKGELTCGKCKKYCYEKIVEFLKDLHEKREAAKEIAYKIINNQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCX7</accession>
    <name>F6BCX7_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Beta-lactamase domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0793</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96338.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798941.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0793"/>
    <dbReference type="EnsemblBacteria" id="AEF96338">
        <property type="protein sequence ID" value="AEF96338"/>
        <property type="gene ID" value="Metig_0793"/>
    </dbReference>
    <dbReference type="GeneID" id="10643633"/>
    <dbReference type="KEGG" id="mig:Metig_0793"/>
    <dbReference type="HOGENOM" id="CLU_036012_0_0_2"/>
    <dbReference type="OMA" id="GHYDHTD"/>
    <dbReference type="OrthoDB" id="73329at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd07713">
        <property type="entry name" value="DHPS-like_MBL-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041712">
        <property type="entry name" value="DHPS-like_MBL-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="SSF56281"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Lactamase_B" evidence="1">
        <location>
            <begin position="19"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96338.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="259" mass="29912" checksum="42F3B1B672346D87" modified="2011-07-27" version="1">MQIKILIDNIAYKRYLAQHGLSIIIEGKKRILFDTGQNPLTLKKNLEIMGEDDSFDAIVLSHGHYDHADGLFYFINKEDFNTQIIMHPDALLDKYDENKRRYIGINKEIKEFLKNYSNTLFVEEPYKLSKDIIISGYIPRKISCEMEKFYTLMDGEFKEDFVNDDMFLIVDGVVITGCSHSGIVNVIEYAKSLSKIRGVIGGFHLVNASERYINEVKEYLKNQNLEFIMPLHCTGFKAMKILSDLDNFVYGHVGKVIEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCY1</accession>
    <name>F6BCY1_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Prefoldin subunit alpha</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">GimC subunit alpha</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">pfdA</name>
        <name evidence="7" type="ordered locus">Metig_0797</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4 5">Molecular chaperone capable of stabilizing a range of proteins. Seems to fulfill an ATP-independent, HSP70-like function in archaeal de novo protein folding.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 5">Heterohexamer of two alpha and four beta subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the prefoldin alpha subunit family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the prefoldin subunit alpha family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96342.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798945.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0797"/>
    <dbReference type="EnsemblBacteria" id="AEF96342">
        <property type="protein sequence ID" value="AEF96342"/>
        <property type="gene ID" value="Metig_0797"/>
    </dbReference>
    <dbReference type="GeneID" id="10643638"/>
    <dbReference type="KEGG" id="mig:Metig_0797"/>
    <dbReference type="HOGENOM" id="CLU_091867_1_2_2"/>
    <dbReference type="OMA" id="ELQNQFM"/>
    <dbReference type="OrthoDB" id="105878at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016272">
        <property type="term" value="C:prefoldin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00308">
        <property type="entry name" value="PfdA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011599">
        <property type="entry name" value="PFD_alpha_archaea"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009053">
        <property type="entry name" value="Prefoldin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004127">
        <property type="entry name" value="Prefoldin_subunit_alpha"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12674">
        <property type="entry name" value="PTHR12674"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02996">
        <property type="entry name" value="Prefoldin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00293">
        <property type="entry name" value="TIGR00293"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0143">Chaperone</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="6"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="87"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00025077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEF96342.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="143" mass="16431" checksum="8F4076D12488F7A2" modified="2011-07-27" version="1">MNEEEIRQKLAVLETYNQQIQKLQEELVNIGLMKNEINKAIETLEGIKENDEVLFPIGAGAFVKAKVLEKDKVIVGIGANIFVDKNIDEVIKEFKKSVEDIEKAEAKGKEQIENTIKVARKIQKELEKELARIQKEQQEQQQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-09-29" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>F6BCZ4</accession>
    <name>F6BCZ4_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="3">DNA polymerase beta domain protein region</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_0810</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96355.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798957.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96355">
        <property type="protein sequence ID" value="AEF96355"/>
        <property type="gene ID" value="Metig_0810"/>
    </dbReference>
    <dbReference type="GeneID" id="10643651"/>
    <dbReference type="KEGG" id="mig:Metig_0810"/>
    <dbReference type="HOGENOM" id="CLU_130257_10_2_2"/>
    <dbReference type="OMA" id="YNSIHPL"/>
    <dbReference type="OrthoDB" id="108900at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041633">
        <property type="entry name" value="Polbeta"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18765">
        <property type="entry name" value="Polbeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="SSF81301"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Polbeta" evidence="2">
        <location>
            <begin position="9"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF18765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF96355.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="93" mass="10700" checksum="BFB1DADE1C729B78" modified="2011-07-27" version="1">MNINEIKRKILPILLKYNVKKASIFGSYARGEQKETSDIDILVEFGEGKSLLDLVRLKYELEEVLGKKVDILTYNSIHPLLKERILNEAVNIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>F6BD13</accession>
    <name>F6BD13_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0829</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96374.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798976.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0829"/>
    <dbReference type="EnsemblBacteria" id="AEF96374">
        <property type="protein sequence ID" value="AEF96374"/>
        <property type="gene ID" value="Metig_0829"/>
    </dbReference>
    <dbReference type="GeneID" id="10643670"/>
    <dbReference type="KEGG" id="mig:Metig_0829"/>
    <dbReference type="HOGENOM" id="CLU_075000_0_0_2"/>
    <dbReference type="OMA" id="MPVRIYE"/>
    <dbReference type="OrthoDB" id="115684at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10900">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004425">
        <property type="entry name" value="CHP00061"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019151">
        <property type="entry name" value="Proteasome_assmbl_chaperone_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038389">
        <property type="entry name" value="PSMG2_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09754">
        <property type="entry name" value="PAC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00161">
        <property type="entry name" value="TIGR00161"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="199"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96374.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="236" mass="25916" checksum="80398320AD0DEC9E" modified="2011-07-27" version="1">MEYIAKKEIDFKNPLVIEGFPGVGLVGSIAAYQIIKKLELECIGYFDVPEAPPVNLVEEGVVYPPIRVYGREDLIVLFSDVIIPPVIVHKLSDKIVEILTPINPQIVVSLGGLATGKSEKVYGIASSKELLEIFEKYDIPILKFGMVGGVSGSLIVKSNNNKIPAIGLLAETVGIRPDPRGASNLVNVLNKMYGLNVNVEELIEEADKIDEKIKQLAQEHAKLMTKTARSEYPMYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>F6BD41</accession>
    <name>F6BD41_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF43 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0858</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96402.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799004.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0858"/>
    <dbReference type="EnsemblBacteria" id="AEF96402">
        <property type="protein sequence ID" value="AEF96402"/>
        <property type="gene ID" value="Metig_0858"/>
    </dbReference>
    <dbReference type="GeneID" id="10643699"/>
    <dbReference type="KEGG" id="mig:Metig_0858"/>
    <dbReference type="HOGENOM" id="CLU_042160_0_0_2"/>
    <dbReference type="OMA" id="ECGGKKF"/>
    <dbReference type="OrthoDB" id="24160at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016765">
        <property type="term" value="F:transferase activity, transferring alkyl or aryl (other than methyl) groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044260">
        <property type="term" value="P:cellular macromolecule metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043412">
        <property type="term" value="P:macromolecule modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006596">
        <property type="term" value="P:polyamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044238">
        <property type="term" value="P:primary metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014435">
        <property type="entry name" value="BpsA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002723">
        <property type="entry name" value="BpsA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002052">
        <property type="entry name" value="DNA_methylase_N6_adenine_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01861">
        <property type="entry name" value="DUF43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005895">
        <property type="entry name" value="UCP005895_mtase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00092">
        <property type="entry name" value="N6_MTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF43" evidence="1">
        <location>
            <begin position="130"/>
            <end position="379"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96402.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="391" mass="44364" checksum="4F2BE0136EF1A8BD" modified="2011-07-27" version="1">MKIIGKIGKGKKEVEEKDVIKPILLKEVARKADIAEGERAVEDILRCIYRNQPISTKKIGQYVKLPLPIVAKVRTLLERAKILDREEKGAVLTEIGKEFVEKELKLKMKHDLTCPLCNGKGIVLDEMFEEILKKQKEYAKKRPIANTTIDQSYATPETAIYRAALMAQRGDLEGKRVLFVGDDDLTSIPTALTNLCEEVVVVDIDERLLKLIDDVSKKENLNIKTIKHDLRNPLPEKLKNKFDVIFTDPPYTLNGMKLFLSRGIEGLGDEGVAYLAFSHKPIDEWIEVQKTLSEMGFVIYELIPGFNLYEGSEIIGNTTFLARLIGKNLKPIIGDTEKIYTGEVKPTLRYYKCLKCGKIHEVGDKIKRVEDLICECGGQKFSMVKRKKIKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>F6BD62</accession>
    <name>F6BD62_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ACT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0880</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96423.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96423">
        <property type="protein sequence ID" value="AEF96423"/>
        <property type="gene ID" value="Metig_0880"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0880"/>
    <dbReference type="HOGENOM" id="CLU_1792142_0_0_2"/>
    <dbReference type="OMA" id="IWLVIEN"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002912">
        <property type="entry name" value="ACT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030489">
        <property type="entry name" value="TR_Rrf2-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000944">
        <property type="entry name" value="Tscrpt_reg_Rrf2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33221">
        <property type="entry name" value="PTHR33221"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02082">
        <property type="entry name" value="Rrf2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="SSF55021"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51671">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01332">
        <property type="entry name" value="HTH_RRF2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ACT" evidence="1">
        <location>
            <begin position="112"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96423.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="178" mass="20281" checksum="79EF663BFEB93687" modified="2011-07-27" version="1">MKQQTNIITQITIKRNKQIFLKSILTLTTLHSEKMKVSGLTKKIMELLKTCSTTKEIAEKLNMHPKNIDRHIRTLRDLGLVETKKGKKGGIKLTKDGKYLLEKGEINLSIIKVQIVAKDRIGLLAEITSKISEINGNILSTTLEKDNEDVIIWLVIENVDIDELREKLKDTVKKLIIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>F6BD87</accession>
    <name>F6BD87_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">50S ribosomal protein L5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">rpl5</name>
        <name evidence="8" type="ordered locus">Metig_0905</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">This is 1 of the proteins that binds and probably mediates the attachment of the 5S RNA into the large ribosomal subunit, where it forms part of the central protuberance. In the 70S ribosome it contacts protein S13 of the 30S subunit (bridge B1b), connecting the 2 subunits; this bridge is implicated in subunit movement. May contact the P site tRNA; the 5S rRNA and some of its associated proteins might help stabilize positioning of ribosome-bound tRNAs.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Part of the 50S ribosomal subunit; contacts the 5S rRNA and probably tRNA. Forms a bridge to the 30S subunit in the 70S ribosome.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4 5">Belongs to the universal ribosomal protein uL5 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96448.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799050.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0905"/>
    <dbReference type="EnsemblBacteria" id="AEF96448">
        <property type="protein sequence ID" value="AEF96448"/>
        <property type="gene ID" value="Metig_0905"/>
    </dbReference>
    <dbReference type="GeneID" id="10643749"/>
    <dbReference type="KEGG" id="mig:Metig_0905"/>
    <dbReference type="HOGENOM" id="CLU_061015_3_0_2"/>
    <dbReference type="OMA" id="ERMYAFL"/>
    <dbReference type="OrthoDB" id="97230at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1440.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01333_A">
        <property type="entry name" value="Ribosomal_L5_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002132">
        <property type="entry name" value="Ribosomal_L5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022804">
        <property type="entry name" value="Ribosomal_L5_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031309">
        <property type="entry name" value="Ribosomal_L5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020929">
        <property type="entry name" value="Ribosomal_L5_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022803">
        <property type="entry name" value="Ribosomal_L5_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031310">
        <property type="entry name" value="Ribosomal_L5_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11994">
        <property type="entry name" value="PTHR11994"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00281">
        <property type="entry name" value="Ribosomal_L5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00673">
        <property type="entry name" value="Ribosomal_L5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002161">
        <property type="entry name" value="Ribosomal_L5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55282">
        <property type="entry name" value="SSF55282"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00358">
        <property type="entry name" value="RIBOSOMAL_L5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 5" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="4" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0699">rRNA-binding</keyword>
    <keyword evidence="4" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="Ribosomal_L5" evidence="6">
        <location>
            <begin position="10"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="domain" description="Ribosomal_L5_C" evidence="7">
        <location>
            <begin position="67"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEF96448.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="182" mass="20942" checksum="625DEB4CC1053E1D" modified="2011-07-27" version="1">MSYQELWKNNPMLKPRIEKVVVNFGVGEAGDRLTKGAKVMEEITGQKPIRTLAKQTNPAFGIRKKLPIGLKVTLRGKKAEEFLKNAFIAFKAEGKKLYESSFDEYGNFSFGIHEHIDFPGQKYDPMIGIYGMDVCVTLERPGYRVKRRKVKRARIPKRHRVTKEEAIEFIEKHFGVEVVRGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>F6BD97</accession>
    <name>F6BD97_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 7">Elongation factor 1-beta</fullName>
            <shortName evidence="7">EF-1-beta</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6 7">aEF-1beta</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">ef1b</name>
        <name evidence="9" type="ordered locus">Metig_0915</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 7">Promotes the exchange of GDP for GTP in EF-1-alpha/GDP, thus allowing the regeneration of EF-1-alpha/GTP that could then be used to form the ternary complex EF-1-alpha/GTP/AAtRNA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the EF-1-beta/EF-1-delta family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96458.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799060.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0915"/>
    <dbReference type="EnsemblBacteria" id="AEF96458">
        <property type="protein sequence ID" value="AEF96458"/>
        <property type="gene ID" value="Metig_0915"/>
    </dbReference>
    <dbReference type="GeneID" id="10643759"/>
    <dbReference type="KEGG" id="mig:Metig_0915"/>
    <dbReference type="HOGENOM" id="CLU_165896_0_0_2"/>
    <dbReference type="OMA" id="VMPNSPE"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00043">
        <property type="entry name" value="EF1_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036219">
        <property type="entry name" value="eEF-1beta-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014038">
        <property type="entry name" value="EF1B_bsu/dsu_GNE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014717">
        <property type="entry name" value="Transl_elong_EF1B/ribosomal_S6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004542">
        <property type="entry name" value="Transl_elong_EF1B_B_arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39647">
        <property type="entry name" value="PTHR39647"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00736">
        <property type="entry name" value="EF1_GNE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006521">
        <property type="entry name" value="Transl_elong_EF1B_B_arc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00888">
        <property type="entry name" value="EF1_GNE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54984">
        <property type="entry name" value="SSF54984"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00489">
        <property type="entry name" value="aEF-1_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7 9" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="5 7" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EF1_GNE" evidence="8">
        <location>
            <begin position="3"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00032274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AEF96458.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="89" mass="9836" checksum="772529530EAA00C1" modified="2011-07-27" version="1">MAKVLAKIKVMPTSPEVNKEELKEKVKETIEKTDAVCRGVSDEPLAFGLYAVYVVVEMEEKEGGTEPIEEALRNLENVESVEVVEVSLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDD5</accession>
    <name>F6BDD5_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_0953</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96496.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96496">
        <property type="protein sequence ID" value="AEF96496"/>
        <property type="gene ID" value="Metig_0953"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0953"/>
    <dbReference type="HOGENOM" id="CLU_219926_0_0_2"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF96496.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="38" mass="4204" checksum="7D38D64B442AC94D" modified="2011-07-27" version="1">MVMAMIPVNISEDALEFIKKKLKQANADSIVIYFAGFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDF3</accession>
    <name>F6BDF3_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Class III signal peptide-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_0971</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96514.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0971"/>
    <dbReference type="EnsemblBacteria" id="AEF96514">
        <property type="protein sequence ID" value="AEF96514"/>
        <property type="gene ID" value="Metig_0971"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_0971"/>
    <dbReference type="HOGENOM" id="CLU_1912362_0_0_2"/>
    <dbReference type="OMA" id="VGYPGMF"/>
    <dbReference type="OrthoDB" id="116062at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007166">
        <property type="entry name" value="Class3_signal_pept_motif"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04021">
        <property type="entry name" value="Class_IIIsignal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF96514.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="141" mass="15154" checksum="BE27EA2E5BDEEB84" modified="2011-07-27" version="1">MQIKGQISLEFTIIILAFLIAMVITTMGPGLFGMDKSVKTASASLAHAAMSKLKTNIELLAVADEGSSKIVYIKSPPGNWTVDGNNITFIGNDYNVSTICSKRLKLIVNGTEKNSFTVPTMSIIGCKLTRDGDKVVINITQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-05-25" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDH0</accession>
    <name>F6BDH0_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 3">30S ribosomal protein S27e</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">rps27e</name>
        <name evidence="4" type="ordered locus">Metig_0989</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="3">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the eukaryotic ribosomal protein eS27 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96531.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799133.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0989"/>
    <dbReference type="EnsemblBacteria" id="AEF96531">
        <property type="protein sequence ID" value="AEF96531"/>
        <property type="gene ID" value="Metig_0989"/>
    </dbReference>
    <dbReference type="GeneID" id="10643836"/>
    <dbReference type="KEGG" id="mig:Metig_0989"/>
    <dbReference type="HOGENOM" id="CLU_199465_0_0_2"/>
    <dbReference type="OMA" id="DCENEQV"/>
    <dbReference type="OrthoDB" id="129447at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00371">
        <property type="entry name" value="Ribosomal_S27e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000592">
        <property type="entry name" value="Ribosomal_S27"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023407">
        <property type="entry name" value="Ribosomal_S27_Zn-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011332">
        <property type="entry name" value="Ribosomal_zn-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01667">
        <property type="entry name" value="Ribosomal_S27e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57829">
        <property type="entry name" value="SSF57829"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 4" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="zinc finger region" description="C4-type" evidence="3">
        <location>
            <begin position="16"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00029526"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEF96531.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="61" mass="6614" checksum="5A65B2E424B2F7EF" modified="2011-07-27" version="1">MDLIPTPRSKFLKVQCPECNNEQVIFGCPSTVVKCLVCGKVLAEPRASKGLIKAKILEVLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDJ6</accession>
    <name>F6BDJ6_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Xylose isomerase domain-containing protein TIM barrel</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1015</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96557.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799159.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1015"/>
    <dbReference type="EnsemblBacteria" id="AEF96557">
        <property type="protein sequence ID" value="AEF96557"/>
        <property type="gene ID" value="Metig_1015"/>
    </dbReference>
    <dbReference type="GeneID" id="10643869"/>
    <dbReference type="KEGG" id="mig:Metig_1015"/>
    <dbReference type="HOGENOM" id="CLU_068832_0_0_2"/>
    <dbReference type="OMA" id="HIHISGI"/>
    <dbReference type="OrthoDB" id="51470at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00019">
        <property type="entry name" value="AP2Ec"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001719">
        <property type="entry name" value="AP_endonuc_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036237">
        <property type="entry name" value="Xyl_isomerase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013022">
        <property type="entry name" value="Xyl_isomerase-like_TIM-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21445">
        <property type="entry name" value="PTHR21445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01261">
        <property type="entry name" value="AP_endonuc_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00518">
        <property type="entry name" value="AP2Ec"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51658">
        <property type="entry name" value="SSF51658"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0413">Isomerase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AP_endonuc_2" evidence="1">
        <location>
            <begin position="22"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96557.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="270" mass="30760" checksum="85A269E68F208F09" modified="2011-07-27" version="1">MGLKFGTAGIPINAKSNYDAITFLRKVGLNALELEFVRGVNMKEDYAKKLKSHAKEDVVLSAHAPYYINLNAKEEDKIEGSIRRIVNTAKITNVCGDNVVFHPGYYLKKNKEEVYKKIVSNIKRILDIMEENKIKSMLRPETTGKISQFGSIDEIIDLCYELNILPCVDFAHIYARSLGKINDYNSFHNILSKIEDKLGKDAIKNMHIHMSGIDFGKGGERSHLPLKESKFNYKDVLKVLKDFDVKGVVICESPKLEYDALLMKKYYESL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDL8</accession>
    <name>F6BDL8_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glutamate synthase alpha subunit domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1037</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96579.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799181.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1037"/>
    <dbReference type="EnsemblBacteria" id="AEF96579">
        <property type="protein sequence ID" value="AEF96579"/>
        <property type="gene ID" value="Metig_1037"/>
    </dbReference>
    <dbReference type="GeneID" id="10643891"/>
    <dbReference type="KEGG" id="mig:Metig_1037"/>
    <dbReference type="HOGENOM" id="CLU_078510_1_0_2"/>
    <dbReference type="OMA" id="PYIEEFC"/>
    <dbReference type="OrthoDB" id="55773at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00981">
        <property type="entry name" value="arch_gltB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035710">
        <property type="entry name" value="Archaeal_gltB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002489">
        <property type="entry name" value="Glu_synth_asu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036485">
        <property type="entry name" value="Glu_synth_asu_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012061">
        <property type="entry name" value="Glu_synth_lsu_3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01493">
        <property type="entry name" value="GXGXG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006519">
        <property type="entry name" value="GOGAT_dom3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69336">
        <property type="entry name" value="SSF69336"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GXGXG" evidence="1">
        <location>
            <begin position="11"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96579.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="258" mass="28605" checksum="2DF26D8CE77432E7" modified="2011-07-27" version="1">MKEITIDAKGMDYRELNEKIHEALRENPDLEKIVLKNVLGQRFIGNGIQKKNLTIEIYGVPGGDLGMFMNGPKIIVYGNTDHAPGNTLDDGVIVIHGSGGDVTGHSMRGGKIFVRDNVGYRSGIHMKEYKDKVPVLVIGGTAKDFLGEYMAGGIIIVLNIDREGNDLGKIKGRFIGTGIHGGSIYIRGDVDRFQLGVAADIKEFTEEDREKIKPYIEEFCKEFGLSDEIKEKLINSKYTKIAPISKRPFGKLYTPDLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2021-09-29" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDQ3</accession>
    <name>F6BDQ3_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1075</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96614.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799215.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AEF96614">
        <property type="protein sequence ID" value="AEF96614"/>
        <property type="gene ID" value="Metig_1075"/>
    </dbReference>
    <dbReference type="GeneID" id="10643929"/>
    <dbReference type="KEGG" id="mig:Metig_1075"/>
    <dbReference type="HOGENOM" id="CLU_989056_0_0_2"/>
    <dbReference type="OMA" id="PLYWMAN"/>
    <dbReference type="OrthoDB" id="128926at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="59"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="90"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="114"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="154"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="194"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="235"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="258"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96614.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="281" mass="32765" checksum="BE77DCFC9FDBB32B" modified="2011-07-27" version="1">MIDNIKDRELFDIKKVGIISIMVWFLLNVLAIPLSFSMIISLFGKTWLSGNNYFIENEVGYFTFLISSILAVLSVSYYYSKGKVKALSGYLLKITSMLLISKIALYVISVYYPAYISLLGISNGFICYVVFMAMFIKISELYGKKLSFLDRIKIIALSLLIYLAITYPLYWGIYTFITEDYFTYPSDYWHFEKFIFFIYVLGALISVPSSTYIYSKLTNLGDLKKYSLEMLKYSIVFSVITLISFWAFTLYYHHVFSIGSFIVWLIIYSLIMIFKMLDIER</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDQ7</accession>
    <name>F6BDQ7_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metig_1079</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96618.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799219.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1079"/>
    <dbReference type="EnsemblBacteria" id="AEF96618">
        <property type="protein sequence ID" value="AEF96618"/>
        <property type="gene ID" value="Metig_1079"/>
    </dbReference>
    <dbReference type="GeneID" id="10643933"/>
    <dbReference type="KEGG" id="mig:Metig_1079"/>
    <dbReference type="HOGENOM" id="CLU_140136_0_0_2"/>
    <dbReference type="OMA" id="LFKLAFK"/>
    <dbReference type="OrthoDB" id="90556at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018665">
        <property type="entry name" value="DUF2122_RecB-nuclease-rel"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09895">
        <property type="entry name" value="DUF2122"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEF96618.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="145" mass="15791" checksum="B3F65FDB89F8F6ED" modified="2011-07-27" version="1">MFIALHNTYSAKQVEEFARIVFGFGVDTIVITKATGSAAQSGVPVAHKLACRLGKNVLFFEDLDDAIEVLRPEKVILIGNKETCDKEVDFNSIGKNDLVVFCGASTGFTKLELEKGEGVYLTENEVGAIGGVAIFLYKMKELQLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDW1</accession>
    <name>F6BDW1_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Transcription initiation factor IIB</fullName>
            <shortName evidence="8">TFIIB</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">tfb</name>
        <name evidence="12" type="ordered locus">Metig_1133</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Stabilizes TBP binding to an archaeal box-A promoter. Also responsible for recruiting RNA polymerase II to the pre-initiation complex (DNA-TBP-TFIIB).</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the TFIIB family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96672.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799273.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1133"/>
    <dbReference type="EnsemblBacteria" id="AEF96672">
        <property type="protein sequence ID" value="AEF96672"/>
        <property type="gene ID" value="Metig_1133"/>
    </dbReference>
    <dbReference type="GeneID" id="10643991"/>
    <dbReference type="KEGG" id="mig:Metig_1133"/>
    <dbReference type="HOGENOM" id="CLU_043736_0_1_2"/>
    <dbReference type="OMA" id="DHDQRMK"/>
    <dbReference type="OrthoDB" id="35979at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017025">
        <property type="term" value="F:TBP-class protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070897">
        <property type="term" value="P:transcription preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00043">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00383">
        <property type="entry name" value="TF2B_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000812">
        <property type="entry name" value="TFIIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023484">
        <property type="entry name" value="TFIIB_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023486">
        <property type="entry name" value="TFIIB_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013150">
        <property type="entry name" value="TFIIB_cyclin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013137">
        <property type="entry name" value="Znf_TFIIB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11618">
        <property type="entry name" value="PTHR11618"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08271">
        <property type="entry name" value="TF_Zn_Ribbon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00382">
        <property type="entry name" value="TFIIB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00685">
        <property type="entry name" value="TIFACTORIIB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="SSF47954"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00782">
        <property type="entry name" value="TFIIB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51134">
        <property type="entry name" value="ZF_TFIIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="2 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0677">Repeat</keyword>
    <keyword evidence="7 8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6 8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5 8" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="TFIIB-type" evidence="11">
        <location>
            <begin position="43"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="repeat" description="1" evidence="8">
        <location>
            <begin position="160"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="repeat" description="2" evidence="8">
        <location>
            <begin position="254"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="47"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="50"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="66"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="69"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AEF96672.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="343" mass="38514" checksum="39DC79BBC78F56D9" modified="2011-07-27" version="1">MTTKQELKEKITTTENEKSEKKIKTTNNEKIKSDKNVILEKEEELVCPICGSTNVIKDYERAEIVCENCGCVLQQNLFDVGPEWRAFDHEQRVKRSRVGAPMTYTIHDKGLSTVIDWRNKDSYGKDISANKRAQLYRLRKWQRRIRVSDASERNLAFALSELDRIASKLGLPRNVRENAAVLYRGAVEKGLIRGRSIEGVAAAALYAACRRCRVPRTLDEIAEASRVDRKEIGRTYRFLARELGIKLAPTNPIDYVPRFASELGLPGEVESKAIAILQKAAEKGLTSGRGPTGVAAAAIYIASVLLGNRRTQRDVAEVAGVTEVTIRNRYKELTEHLDIDVTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>F6BDY7</accession>
    <name>F6BDY7_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glutamate synthase alpha subunit domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1159</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96698.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799299.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1159"/>
    <dbReference type="EnsemblBacteria" id="AEF96698">
        <property type="protein sequence ID" value="AEF96698"/>
        <property type="gene ID" value="Metig_1159"/>
    </dbReference>
    <dbReference type="GeneID" id="10644017"/>
    <dbReference type="KEGG" id="mig:Metig_1159"/>
    <dbReference type="HOGENOM" id="CLU_867712_0_0_2"/>
    <dbReference type="OMA" id="FTYNFIW"/>
    <dbReference type="OrthoDB" id="67919at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002489">
        <property type="entry name" value="Glu_synth_asu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036485">
        <property type="entry name" value="Glu_synth_asu_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01493">
        <property type="entry name" value="GXGXG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69336">
        <property type="entry name" value="SSF69336"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GXGXG" evidence="1">
        <location>
            <begin position="190"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96698.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="313" mass="34813" checksum="F54111D7DC498263" modified="2011-07-27" version="1">MVFGIFKKLKKKSIEDFLKDKDILTIKLEKPLDCLCDFTYNFIWQSNFDYNQKVVDDITYKDLVEHLKNKGVVYIKGDVGKRFCSSMGADLKYFGGKGGKIDVGTVVIDGNIDTRFGISMVSGTVYVNEKSTIKEPIGNVIEVESDIKGYRKFISITEFVEKRHNEKLLKPNKFRNDELIINDGIVRDTVGARLEKDATIVVNGNVDLSTGILMKKGKVIVNGNAGKNTGAVLRGGCVIVRGNTGDFTAIEMKKGFIIVDGNAGKYLGAKKKGGVIYARDGSPIPPTKKYPLSEEDKKLLNKLGFFGEFYKFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>F6BE36</accession>
    <name>F6BE36_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Phosphoadenosine phosphosulfate reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_0010</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95572.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798182.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0010"/>
    <dbReference type="EnsemblBacteria" id="AEF95572">
        <property type="protein sequence ID" value="AEF95572"/>
        <property type="gene ID" value="Metig_0010"/>
    </dbReference>
    <dbReference type="GeneID" id="10642845"/>
    <dbReference type="KEGG" id="mig:Metig_0010"/>
    <dbReference type="HOGENOM" id="CLU_026622_0_0_2"/>
    <dbReference type="OMA" id="RWCCKVT"/>
    <dbReference type="OrthoDB" id="6490at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01713">
        <property type="entry name" value="PAPS_reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.130.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002500">
        <property type="entry name" value="PAPS_reduct"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002478">
        <property type="entry name" value="PUA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036974">
        <property type="entry name" value="PUA_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004521">
        <property type="entry name" value="Uncharacterised_CHP00451"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01507">
        <property type="entry name" value="PAPS_reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01472">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00359">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="SSF88697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00451">
        <property type="entry name" value="unchar_dom_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PUA" evidence="1">
        <location>
            <begin position="124"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF95572.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="480" mass="55906" checksum="97E0271D28AEB76B" modified="2011-07-27" version="1">MRTILGKMHLKWCHYCNLPVLDKICNICGNEPVEVKLTPPGGARPAFEKEIEFIENLIKEQFGTKEDILKGRIVLMNKLPGNEDMQEIIIDGQVIAVLKYDNGWKIMPRLEGARRIVNAGGYKKIVVIKEDVIPYLLGRSSSVLRPGVTYVSEGIKKEDEVIILKKMNGKDEDVVAVGRAKMDYEEILNAEKGVVVKVRHAEEPRKANELKTSEKNLRELFKDMVKANEDVIRRFEENSVGFMRNTAKRINLPITVAYSGGKDSLTVLLLALKAFDKEEFDVLFTDTGLEFPETIKNVDIVQKTYGIKLIKEESNEFWEKLEEYGPPGRDYRWCSEICKLKPLKRLIDKHYPNGTLTFVGLRKYESFSRAKKPRVQKNPHIPKQINAMPIHNWSAMHVWLYILKNNAPYNKMYEKGFDRVGCYVCPAMELGEIDKVKREYPELWEKWEKFLRKWAEKHGYGEKWIKELWRWKNPNKRKNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>F6BE74</accession>
    <name>F6BE74_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF3343 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1213</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96751.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1213"/>
    <dbReference type="EnsemblBacteria" id="AEF96751">
        <property type="protein sequence ID" value="AEF96751"/>
        <property type="gene ID" value="Metig_1213"/>
    </dbReference>
    <dbReference type="KEGG" id="mig:Metig_1213"/>
    <dbReference type="HOGENOM" id="CLU_117547_0_0_2"/>
    <dbReference type="OMA" id="GCPDVPY"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021778">
        <property type="entry name" value="Se/S_carrier-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11823">
        <property type="entry name" value="DUF3343"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF3343" evidence="1">
        <location>
            <begin position="29"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF11823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96751.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="189" mass="21411" checksum="29171FC5D7E4C35D" modified="2011-07-27" version="1">MRGEIVILKKIKSLLEKGDKKEEKEFEGKGLIIFKSVKDAMKAENILRGYNIKVVAPPHEIREGCDLAIEYDLIDELGIKRELEKNNIEPLKFISLNDYSLKPLELIKIKEVDGFVMVRCGNMKITIDKEGNIVNISGGGCPDVPYLALKLKGRNIKDIKEDETPKSLGYTLCAYTLNKAFEKARELVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEH9</accession>
    <name>F6BEH9_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF190 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metig_0079</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0166 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95640.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798249.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0079"/>
    <dbReference type="EnsemblBacteria" id="AEF95640">
        <property type="protein sequence ID" value="AEF95640"/>
        <property type="gene ID" value="Metig_0079"/>
    </dbReference>
    <dbReference type="GeneID" id="10642914"/>
    <dbReference type="KEGG" id="mig:Metig_0079"/>
    <dbReference type="HOGENOM" id="CLU_146749_2_0_2"/>
    <dbReference type="OMA" id="EHWNTLR"/>
    <dbReference type="OrthoDB" id="122737at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003793">
        <property type="entry name" value="DUF190"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011322">
        <property type="entry name" value="N-reg_PII-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015867">
        <property type="entry name" value="N-reg_PII/ATP_PRibTrfase_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02641">
        <property type="entry name" value="DUF190"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54913">
        <property type="entry name" value="SSF54913"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF190" evidence="2">
        <location>
            <begin position="5"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEF95640.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="104" mass="11899" checksum="56FCE4B7176BFC69" modified="2011-07-27" version="1">MIKAKLLRIYLRENDRHGDEPLYKYIIRILKENNISGATVFKGMCGYGVRGISRVDILRLSMNLPVVIECVDKEENISKVLPKIIDVVGDNGLVYVMDVEVYKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEM7</accession>
    <name>F6BEM7_METIK</name>
    <protein>
        <submittedName>
            <fullName evidence="11">HhH-GPD family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">Metig_1287</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Nth/MutY family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96824.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799421.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1287"/>
    <dbReference type="EnsemblBacteria" id="AEF96824">
        <property type="protein sequence ID" value="AEF96824"/>
        <property type="gene ID" value="Metig_1287"/>
    </dbReference>
    <dbReference type="GeneID" id="10644150"/>
    <dbReference type="KEGG" id="mig:Metig_1287"/>
    <dbReference type="HOGENOM" id="CLU_012862_6_0_2"/>
    <dbReference type="OMA" id="GPQGWWP"/>
    <dbReference type="OrthoDB" id="58645at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016798">
        <property type="term" value="F:hydrolase activity, acting on glycosyl bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00056">
        <property type="entry name" value="ENDO3c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1670.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011257">
        <property type="entry name" value="DNA_glycosylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003651">
        <property type="entry name" value="Endonuclease3_FeS-loop_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004035">
        <property type="entry name" value="Endouclease-III_FeS-bd_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003265">
        <property type="entry name" value="HhH-GPD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023170">
        <property type="entry name" value="HhH_base_excis_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000445">
        <property type="entry name" value="HhH_motif"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10576">
        <property type="entry name" value="EndIII_4Fe-2S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00633">
        <property type="entry name" value="HHH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00730">
        <property type="entry name" value="HhH-GPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00478">
        <property type="entry name" value="ENDO3c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00525">
        <property type="entry name" value="FES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48150">
        <property type="entry name" value="SSF48150"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00764">
        <property type="entry name" value="ENDONUCLEASE_III_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0227">DNA damage</keyword>
    <keyword evidence="8" id="KW-0234">DNA repair</keyword>
    <keyword evidence="9" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="7" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ENDO3c" evidence="10">
        <location>
            <begin position="34"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AEF96824.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="220" mass="25737" checksum="022EF352049F850D" modified="2011-07-27" version="1">MELLKIYHLLLSHFGYQNWWPAETRYEVVIGAVLTQNTSWKNVEKAIENLKKENLIDERKILEIDTEKLKKLIKPAGFYNIKAERLKNITHHIVKNYGSTENLAKLPIKLEDLRKELLNVKGIGKETADSILLYALDRPIFVVDAYTKRIFSRLGVIEGGEEYDEIRHIFEENLPKDLKIYKEYHALIVELGKHYCKKRNPACEKCPLSDLCDYSCLPYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>F6BET9</accession>
    <name>F6BET9_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0212 protein Metig_1351</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metig_1351</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0212 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96886.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799483.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1351"/>
    <dbReference type="EnsemblBacteria" id="AEF96886">
        <property type="protein sequence ID" value="AEF96886"/>
        <property type="gene ID" value="Metig_1351"/>
    </dbReference>
    <dbReference type="GeneID" id="10644214"/>
    <dbReference type="KEGG" id="mig:Metig_1351"/>
    <dbReference type="HOGENOM" id="CLU_138334_0_0_2"/>
    <dbReference type="OMA" id="KVFNAQS"/>
    <dbReference type="OrthoDB" id="101789at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01223">
        <property type="entry name" value="UPF0212"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007564">
        <property type="entry name" value="UPF0212"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42199">
        <property type="entry name" value="PTHR42199"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04475">
        <property type="entry name" value="DUF555"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016934">
        <property type="entry name" value="UCP016934"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEF96886.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="115" mass="12520" checksum="E7600E9739CB8A80" modified="2011-07-27" version="1">MGNYHVTLQAAYVVKNVDDVEDAISVAISQIGRTLNRHGMEYVDIDIGLTICPKCGEPIDCVLVVARTALVGVLLSMRVFNAESTEHAIRIAKATIGKALKDIPLEVVDVVEMRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-01-19" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>F6BEW6</accession>
    <name>F6BEW6_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Cobalt-precorrin-5B C(1)-methyltransferase</fullName>
            <ecNumber evidence="5">2.1.1.195</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Cobalt-precorrin-6A synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">cbiD</name>
        <name evidence="6" type="ordered locus">Metig_0142</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the methylation of C-1 in cobalt-precorrin-5B to form cobalt-precorrin-6A.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>Co-precorrin-5B + S-adenosyl-L-methionine = Co-precorrin-6A + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:26285"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:60063"/>
            <dbReference type="ChEBI" id="CHEBI:60064"/>
            <dbReference type="EC" id="2.1.1.195"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="5">Cofactor biosynthesis; adenosylcobalamin biosynthesis; cob(II)yrinate a,c-diamide from sirohydrochlorin (anaerobic route): step 6/10.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the CbiD family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.195" evidence="5"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF95702.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013798311.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_0142"/>
    <dbReference type="EnsemblBacteria" id="AEF95702">
        <property type="protein sequence ID" value="AEF95702"/>
        <property type="gene ID" value="Metig_0142"/>
    </dbReference>
    <dbReference type="GeneID" id="10642977"/>
    <dbReference type="KEGG" id="mig:Metig_0142"/>
    <dbReference type="HOGENOM" id="CLU_041273_1_0_2"/>
    <dbReference type="OMA" id="YHGKLIK"/>
    <dbReference type="OrthoDB" id="57676at2157"/>
    <dbReference type="UniPathway" id="UPA00148">
        <property type="reaction ID" value="UER00227"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043780">
        <property type="term" value="F:cobalt-precorrin-5B C1-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046140">
        <property type="term" value="P:corrin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00787">
        <property type="entry name" value="CbiD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002748">
        <property type="entry name" value="CbiD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036074">
        <property type="entry name" value="CbiD_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35863">
        <property type="entry name" value="PTHR35863"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01888">
        <property type="entry name" value="CbiD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF026782">
        <property type="entry name" value="CbiD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111342">
        <property type="entry name" value="SSF111342"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00312">
        <property type="entry name" value="cbiD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 5" id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword evidence="2 5" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3 5" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEF95702.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="361" mass="40055" checksum="4371B73B7751F067" modified="2011-07-27" version="1">MIYDFRKKGKLGYTTGSCAAAGAYAGLYFLKNNVRLNYVKIENPNGDILIIPIEKIERIRKNTAKTTVVKYAGEDIDITNGIEIIVEVELIKRREIKIIGGEGIGIVTKDGLQIKKGDYAINPKPREMIKNNLLKLLDEDEGVIVKISAPKGKELAEKTLNPKLGIVGGISILGTTGIVRPMSNEAYRESLVPQIDVALANGYETLIFTPGNIGTKFAKKLLNAEDDQIVEVSNFWGYMLDKAEEKGVKSILVFGHAGKIIKLAAGIFDTHSKVADARNEILTAYASLFIDDKNTLKKILYANTTEEIMEILKEKNVLHGVFNVITKRVVDRLSNRWKINFSCIIIDMKGNILGKYPENLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2022-08-03" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>F6BF40</accession>
    <name>F6BF40_METIK</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Translation initiation factor 2 subunit gamma</fullName>
            <ecNumber evidence="11">3.6.5.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">aIF2-gamma</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">eIF-2-gamma</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">eif2g</name>
        <name evidence="13" type="ordered locus">Metig_1375</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)</name>
        <dbReference type="NCBI Taxonomy" id="880724"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanotorris</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2011-05" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanotorris igneus Kol 5.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Han J."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Peters L."/>
                <person name="Mikhailova N."/>
                <person name="Chertkov O."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Pagani I."/>
                <person name="Sieprawska-Lupa M."/>
                <person name="Whitman W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">DSM 5666 / JCM 11834 / Kol 5</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">eIF-2 functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 11">
            <text>GTP + H2O = GDP + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="11">Heterotrimer composed of an alpha, a beta and a gamma chain.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EIF2G subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.3" evidence="11"/>
    <dbReference type="EMBL" id="CP002737">
        <property type="protein sequence ID" value="AEF96910.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013799506.1">
        <property type="nucleotide sequence ID" value="NC_015562.1"/>
    </dbReference>
    <dbReference type="STRING" id="880724.Metig_1375"/>
    <dbReference type="EnsemblBacteria" id="AEF96910">
        <property type="protein sequence ID" value="AEF96910"/>
        <property type="gene ID" value="Metig_1375"/>
    </dbReference>
    <dbReference type="GeneID" id="10644240"/>
    <dbReference type="KEGG" id="mig:Metig_1375"/>
    <dbReference type="HOGENOM" id="CLU_027154_0_1_2"/>
    <dbReference type="OMA" id="NIGMVGH"/>
    <dbReference type="OrthoDB" id="25592at2157"/>
    <dbReference type="Proteomes" id="UP000009227">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01888">
        <property type="entry name" value="eIF2_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03688">
        <property type="entry name" value="eIF2_gamma_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15490">
        <property type="entry name" value="eIF2_gamma_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00119">
        <property type="entry name" value="eIF_2_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015256">
        <property type="entry name" value="eIF2g_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044127">
        <property type="entry name" value="eIF2g_dom_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044128">
        <property type="entry name" value="eIF2g_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022424">
        <property type="entry name" value="TIF2_gsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009001">
        <property type="entry name" value="Transl_elong_EF1A/Init_IF2_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09173">
        <property type="entry name" value="eIF2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="SSF50447"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50465">
        <property type="entry name" value="SSF50465"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03680">
        <property type="entry name" value="eif2g_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 11" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="6 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 11 13" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="8 11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9 11" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 11" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Tr-type G" evidence="12">
        <location>
            <begin position="6"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="18"/>
            <end position="23"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="18"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="22"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="43"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="45"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="58"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="61"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="73"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="76"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="146"/>
            <end position="149"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="181"/>
            <end position="183"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AEF96910.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000009227"/>
        </source>
    </evidence>
    <sequence length="410" mass="44625" checksum="A2E95647C228B566" modified="2011-07-27" version="1">MATRKQSEVNIGMVGHVDHGKTSLTKALTGVWTDTHSEELKRGISIRLGYADCEIRKCPKCPEPECYTTKEVCPKCGSKTKFLRRVSFVDSPGHETLMATMLSGASLMDGAILVIAANEPCPQPQTKEHLMALDILGVKNIIIVQNKIDLVDEEQAKENYRQIKEFVKGTVAENAPIIPISAHHEANLDVLLKAIQDFIPTPERNPDLPPRMYVARSFDVNKPGTEIKDLKGGVLGGSIIQGVLEVGKEIEIRPGIQVTEGNKTYWKPLRTKIVSLVAGGKSVKKAYPGGLVGVGTELDPSLTKSDYLSGSVLGEPGTLPPVHDRITIKVHLLDRVVGSKEELVIEPLRTNEVLMINVGTATTVGVITSARGDIADIKLKLPICADKGDRVALSRRIGSRWRLIGYGIIQ</sequence>
</entry>
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Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms Distributed under the Creative Commons Attribution (CC BY 4.0) License
</copyright>
</uniprot>